Restriction map for
pFN21AE3633
(Circular) (Six-base) MAPSORT of: pFN21AE3633.seq Check: 2932 from: 1 to: 7171
pFN21AE3633 7171 bp
With 182 enzymes: SgfI *
March 28, 2012 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4981 278
Size: 53 83 186 936 3445 2468
Fragments arranged by size:
3445 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3777 4431 4446 4514 1937
Size: 1840 654 15 68 4594
Fragments arranged by size:
4594 1840 654 68 15
Acc65I G'GTAC_C
Cuts at: 4492 4492
Size: 7171
AclI AA'CG_TT
Cuts at: 1563 5298 5671 1563
Size: 3735 373 3063
Fragments arranged by size:
3735 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3022 3586 3659 3707 5234 6618 6886 1632
Size: 1390 564 73 48 1527 1384 268 1917
Fragments arranged by size:
1917 1527 1390 1384 564 268 73 48
AfeI AGC'GCT
Cuts at: 2852 2852
Size: 7171
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6974
AflIII A'CryG_T
Cuts at: 1203 3180 3182 6070 7047 1203
Size: 1977 2 2888 977 1327
Fragments arranged by size:
2888 1977 1327 977 2
AgeI A'CCGG_T
Cuts at: 6002 6002
Size: 7171
AhdI GACnn_n'nnGTC
Cuts at: 5900 5900
Size: 7171
AleI CACnn'nnGTG
Cuts at: 1166 3672 1166
Size: 2506 4665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2981 1184
Size: 1797 5374
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2210 2242 2210
Size: 32 7139
AlwNI CAG_nnn'CTG
Cuts at: 2449 2530 2713 3124 4387 4914 6486 2449
Size: 81 183 411 1263 527 1572 3134
Fragments arranged by size:
3134 1572 1263 527 411 183 81
ApaLI G'TGCA_C
Cuts at: 5228 6384 5228
Size: 1156 6015
ApoI r'AATT_y
Cuts at: 1493 4480 4706 4858 6967 1493
Size: 2987 226 152 2109 1697
Fragments arranged by size:
2987 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5725 160
Size: 872 4693 1606
Fragments arranged by size:
4693 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
AvaI C'yCGr_G
Cuts at: 1870 1943 2811 2948 3608 3839 4496 1870
Size: 73 868 137 660 231 657 4545
Fragments arranged by size:
4545 868 660 657 231 137 73
BamHI G'GATC_C
Cuts at: 2371 4501 4868 2371
Size: 2130 367 4674
Fragments arranged by size:
4674 2130 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 4136 4492 5947 618
Size: 325 217 627 311 2038 356 1455 1842
Fragments arranged by size:
2038 1842 1455 627 356 325 311 217
BanII G_rGCy'C
Cuts at: 1391 2169 2319 2660 3036 3485 4131 4490 1391
Size: 778 150 341 376 449 646 359 4072
Fragments arranged by size:
4072 778 646 449 376 359 341 150
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2118 928
Size: 971 219 5981
Fragments arranged by size:
5981 971 219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2288 2322 5362 5396 1903
Size: 34 351 34 3040 34 3678
Fragments arranged by size:
3678 3040 351 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5063 6273 5063
Size: 1210 5961
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7171
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3218 3863
Size: 107 122 71 631 605 337 1209 645
Cuts at: 3863 4232 5782 136
Size: 369 1550 1525
Fragments arranged by size:
1550 1525 1209 645 631 605 369 337
122 107 71
BglII A'GATC_T
Cuts at: 7166 7166
Size: 7171
BlpI GC'TnA_GC
Cuts at: 2732 4588 2732
Size: 1856 5315
Bme1580I G_kGCm'C
Cuts at: 1790 2435 2588 5232 6388 1790
Size: 645 153 2644 1156 2573
Fragments arranged by size:
2644 2573 1156 645 153
BmgBI CAC'GTC
Cuts at: 1318 3946 1318
Size: 2628 4543
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 3451 3980
Size: 416 53 83 399 24 897 1137 529
Cuts at: 3980 4362 4902 5860 442
Size: 382 540 958 1753
Fragments arranged by size:
1753 1137 958 897 540 529 416 399
382 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3439 3580 4231 4324 3439
Size: 141 651 93 6286
Fragments arranged by size:
6286 651 141 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 2378 2420 2735 2783 3119 3170 3275
Size: 998 141 42 315 48 336 51 105
Cuts at: 3275 3416 3851 3881 4058 4319 5831 1239
Size: 141 435 30 177 261 1512 2579
Fragments arranged by size:
2579 1512 998 435 336 315 261 177
141 141 105 51 48 42 30
Bpu10I CC'TnA_GC
Cuts at: 2442 2442
Size: 7171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2566 3071 3122 3875 5296 6161 6459 2566
Size: 505 51 753 1421 865 298 3278
Fragments arranged by size:
3278 1421 865 753 505 298 51
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5834 882
Size: 617 4335 2219
Fragments arranged by size:
4335 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2986 3183 4962 493
Size: 711 1782 197 1779 2702
Fragments arranged by size:
2702 1782 1779 711 197
BsaBI GATnn'nnATC
Cuts at: 3465 7165 3465
Size: 3700 3471
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3174 4443 4978 5360
Size: 53 83 186 936 1641 1269 535 382
Cuts at: 5360 5995 275
Size: 635 1451
Fragments arranged by size:
1641 1451 1269 936 635 535 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5604 6002 6276 6423 6834 5604
Size: 398 274 147 411 5941
Fragments arranged by size:
5941 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 2210 2240 2837
Size: 30 165 30 624 30 153 30 597
Cuts at: 2837 2867 1178
Size: 30 5482
Fragments arranged by size:
5482 624 597 165 153 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2234 3044 3746 3770 4139 4169 4172
Size: 991 57 810 702 24 369 30 3
Cuts at: 4172 4262 4265 1186
Size: 90 3 4092
Fragments arranged by size:
4092 991 810 702 369 90 57 30
24 3 3
BseYI C'CCAG_C
Cuts at: 2113 3323 6374 6870 2113
Size: 1210 3051 496 2414
Fragments arranged by size:
3051 2414 1210 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2163 2688 2695 2783 3572 4344 1163
Size: 573 427 525 7 88 789 772 3990
Fragments arranged by size:
3990 789 772 573 525 427 88 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 3961 4440 4621 5382 6410
Size: 275 186 1579 381 479 181 761 1028
Cuts at: 6410 1540
Size: 2301
Fragments arranged by size:
2301 1579 1028 761 479 381 275 186
181
BsiHKAI G_wGCw'C
Cuts at: 2090 2169 2443 3036 3485 4191 5232 5317 6388
Size: 79 274 593 449 706 1041 85 1071
Cuts at: 6388 2090
Size: 2873
Fragments arranged by size:
2873 1071 1041 706 593 449 274 85
79
BsmI GAATG_Cn'
Cuts at: 2287 4682 4775 2287
Size: 2395 93 4683
Fragments arranged by size:
4683 2395 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2169 2319 2435 2443 2588 2660
Size: 399 300 79 150 116 8 145 72
Cuts at: 2660 3036 3485 4131 4191 4490 5232 5317 6388
Size: 376 449 646 60 299 742 85 1071
Cuts at: 6388 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 646 449 399 376 300
299 150 145 116 85 79 72 60
8
BspEI T'CCGG_A
Cuts at: 6834 6834
Size: 7171
BspHI T'CATG_A
Cuts at: 5060 6790 5060
Size: 1730 5441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2388 4386 4407 4526 844
Size: 1544 1998 21 119 3489
Fragments arranged by size:
3489 1998 1544 119 21
BsrBI CCG'CTC
Cuts at: 1394 1823 2767 2887 3564 3613 4162 5058 1394
Size: 429 944 120 677 49 549 896 3507
Fragments arranged by size:
3507 944 896 677 549 429 120 49
BsrDI GCAATG_nn'
Cuts at: 65 1248 5666 5840 65
Size: 1183 4418 174 1396
Fragments arranged by size:
4418 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2090 2104 2619 2634 2643 2954 4439
Size: 110 280 14 515 15 9 311 1485
Cuts at: 4439 5815 6002 1700
Size: 1376 187 2869
Fragments arranged by size:
2869 1485 1376 515 311 280 187 110
15 14 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7171
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7171
BssSI C'ACGA_G
Cuts at: 3128 5231 6243 3128
Size: 2103 1012 4056
Fragments arranged by size:
4056 2103 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3124 4114 4387 794
Size: 2330 990 273 3578
Fragments arranged by size:
3578 2330 990 273
BstBI TT'CG_AA
Cuts at: 5975 5975
Size: 7171
BstEII G'GTnAC_C
Cuts at: 1184 2806 3934 3979 1184
Size: 1622 1128 45 4376
Fragments arranged by size:
4376 1622 1128 45
BstXI CCAn_nnnn'nTGG
Cuts at: 3441 3946 3441
Size: 505 6666
BstYI r'GATC_y
Cuts at: 1972 2371 3618 4501 4868 5253 5270 6722 6808
Size: 399 1247 883 367 385 17 1452 86
Cuts at: 6808 6820 7166 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1247 883 399 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3287 4167 3287
Size: 880 6291
BtgI C'CryG_G
Cuts at: 513 1065 2004 2197 3243 3939 4068 513
Size: 552 939 193 1046 696 129 3616
Fragments arranged by size:
3616 1046 939 696 552 193 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6432
Fragments arranged by size:
6432 683 56
BtsI GCAGTG_nn'
Cuts at: 2686 4687 5481 5501 2686
Size: 2001 794 20 4356
Fragments arranged by size:
4356 2001 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7171
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7136
DraI TTT'AAA
Cuts at: 4475 4475
Size: 7171
DraIII CAC_nnn'GTG
Cuts at: 2806 2806
Size: 7171
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6178 809
Size: 507 4862 1802
Fragments arranged by size:
4862 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3068 3573 3746
Size: 54 1425 50 275 18 1238 505 173
Cuts at: 3746 3806 3954 4365 4437 4618 5507 7136 8
Size: 60 148 411 72 181 889 1629 43
Fragments arranged by size:
1629 1425 1238 889 505 411 275 181
173 148 72 60 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4437 4618 1537
Size: 275 2625 181 4090
Fragments arranged by size:
4090 2625 275 181
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 3041 3551 4268 5101 1353
Size: 801 887 510 717 833 3423
Fragments arranged by size:
3423 887 833 801 717 510
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 3266 3713 4102 5758 6132
Size: 1064 138 453 1224 447 389 1656 374
Cuts at: 6132 6278 387
Size: 146 1280
Fragments arranged by size:
1656 1280 1224 1064 453 447 389 374
146 138
EcoICRI GAG'CTC
Cuts at: 2167 3034 3483 2167
Size: 867 449 5855
Fragments arranged by size:
5855 867 449
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2378 2420 2735 2783 3022 3119
Size: 393 605 141 42 315 48 239 97
Cuts at: 3119 3170 3275 3416 3586 3659 3707 3851 3881
Size: 51 105 141 170 73 48 144 30
Cuts at: 3881 4058 4319 5234 5831 6618 6886 1239
Size: 177 261 915 597 787 268 1524
Fragments arranged by size:
1524 915 787 605 597 393 315 268
261 239 177 170 144 141 141 105
97 73 51 48 48 42 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2801 2801
Size: 7171
EcoO109I rG'GnC_Cy
Cuts at: 3082 3355 3445 3541 3658 3835 3082
Size: 273 90 96 117 177 6418
Fragments arranged by size:
6418 273 177 117 96 90
EcoRI G'AATT_C
Cuts at: 4480 4858 4480
Size: 378 6793
EcoRV GAT'ATC
Cuts at: 3847 3847
Size: 7171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3562 3664 3562
Size: 102 7069
FspI TGC'GCA
Cuts at: 3023 5677 3023
Size: 2654 4517
HaeII r_GCGC'y
Cuts at: 1678 2854 2980 3224 4076 6318 1678
Size: 1176 126 244 852 2242 2531
Fragments arranged by size:
2531 2242 1176 852 244 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2445 2477 2512
Size: 32 135 32 455 32 386 32 35
Cuts at: 2512 2544 5812 5844 5886 5918 1373
Size: 32 3268 32 42 32 2626
Fragments arranged by size:
3268 2626 455 386 135 42 35 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3178 4515 4761 669
Size: 988 281 1240 1337 246 3079
Fragments arranged by size:
3079 1337 1240 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7171
HpaI GTT'AAC
Cuts at: 4761 4761
Size: 7171
KpnI G_GTAC'C
Cuts at: 4496 4496
Size: 7171
MfeI C'AATT_G
Cuts at: 4770 4770
Size: 7171
MluI A'CGCG_T
Cuts at: 7047 7047
Size: 7171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3893 6285 6469 1305
Size: 2588 2392 184 2007
Fragments arranged by size:
2588 2392 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5349
Fragments arranged by size:
5349 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3672 3944 4343 5129 5488 5647 518
Size: 648 2506 272 399 786 359 159 2042
Fragments arranged by size:
2506 2042 786 648 399 359 272 159
MspA1I CmG'CkG
Cuts at: 1606 2139 2662 2973 3124 4029 4587 5264 6412
Size: 533 523 311 151 905 558 677 1148
Cuts at: 6412 6657 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 905 677 558 533 523 311
245 151
NaeI GCC'GGC
Cuts at: 2106 2956 4441 2106
Size: 850 1485 4836
Fragments arranged by size:
4836 1485 850
NcoI C'CATG_G
Cuts at: 513 1065 2004 3243 3939 513
Size: 552 939 1239 696 3745
Fragments arranged by size:
3745 1239 939 696 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7171
NgoMIV G'CCGG_C
Cuts at: 2104 2954 4439 2104
Size: 850 1485 4836
Fragments arranged by size:
4836 1485 850
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7171
NotI GC'GGCC_GC
Cuts at: 4618 4618
Size: 7171
NspI r_CATG'y
Cuts at: 1132 1229 4333 4342 4529 6074 1132
Size: 97 3104 9 187 1545 2229
Fragments arranged by size:
3104 2229 1545 187 97 9
PasI CC'CwG_GG
Cuts at: 1348 3925 1348
Size: 2577 4594
PciI A'CATG_T
Cuts at: 6070 6070
Size: 7171
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2713 1108
Size: 1605 5566
PfoI T'CCnGG_A
Cuts at: 2129 2214 3252 3447 3726 2129
Size: 85 1038 195 279 5574
Fragments arranged by size:
5574 1038 279 195 85
PmeI GTTT'AAAC
Cuts at: 4475 4475
Size: 7171
PmlI CAC'GTG
Cuts at: 2986 3183 2986
Size: 197 6974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2210 2242 5240 5272 6753 6785 2210
Size: 32 2998 32 1481 32 2596
Fragments arranged by size:
2998 2596 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3082 3355 3445 3541 3835 3082
Size: 273 90 96 294 6418
Fragments arranged by size:
6418 294 273 96 90
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7171
PsiI TTA'TAA
Cuts at: 4741 4741
Size: 7171
PstI C_TGCA'G
Cuts at: 830 2244 2256 2655 2922 4107 4383 4392 4404
Size: 1414 12 399 267 1185 276 9 12
Cuts at: 4404 4469 4523 830
Size: 65 54 3478
Fragments arranged by size:
3478 1414 1185 399 276 267 65 54
12 12 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7171
PvuII CAG'CTG
Cuts at: 3124 4029 3124
Size: 905 6266
SacI G_AGCT'C
Cuts at: 2169 3036 3485 2169
Size: 867 449 5855
Fragments arranged by size:
5855 867 449
SalI G'TCGA_C
Cuts at: 1936 4513 1936
Size: 2577 4594
SanDI GG'GwC_CC
Cuts at: 3445 3445
Size: 7171
SapI GCTCTTCn'nnn_
Cuts at: 3041 4268 3041
Size: 1227 5944
SbfI CC_TGCA'GG
Cuts at: 2922 4383 4523 2922
Size: 1461 140 5570
Fragments arranged by size:
5570 1461 140
ScaI AGT'ACT
Cuts at: 3092 5419 7131 3092
Size: 2327 1712 3132
Fragments arranged by size:
3132 2327 1712
SexAI A'CCwGG_T
Cuts at: 2802 2802
Size: 7171
SfcI C'TryA_G
Cuts at: 826 1045 2240 2252 2651 2918 4103 4379 4388
Size: 219 1195 12 399 267 1185 276 9
Cuts at: 4388 4400 4465 4519 5654 6335 6526 826
Size: 12 65 54 1135 681 191 1471
Fragments arranged by size:
1471 1195 1185 1135 681 399 276 267
219 191 65 54 12 12 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 4232 4232
Size: 7171
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7171
SmaI CCC'GGG
Cuts at: 1872 2813 4498 1872
Size: 941 1685 4545
Fragments arranged by size:
4545 1685 941
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2545 3086 3137 3890 5275 6176
Size: 197 926 602 541 51 753 1385 901
Cuts at: 6176 6438 820
Size: 262 1553
Fragments arranged by size:
1553 1385 926 901 753 602 541 262
197 51
SnaBI TAC'GTA
Cuts at: 493 4962 493
Size: 4469 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7171
SphI G_CATG'C
Cuts at: 1132 4529 1132
Size: 3397 3774
SspI AAT'ATT
Cuts at: 5 52 5095 5
Size: 47 5043 2081
Fragments arranged by size:
5043 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7171
StyI C'CwwG_G
Cuts at: 513 1065 2004 3243 3939 4610 513
Size: 552 939 1239 696 671 3074
Fragments arranged by size:
3074 1239 939 696 671 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2200 2526 3395 5198 5215 5368 5553
Size: 1255 620 326 869 1803 17 153 185
Cuts at: 5553 5998 325
Size: 445 1498
Fragments arranged by size:
1803 1498 1255 869 620 445 326 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3090 3988 5417
Size: 274 80 33 51 1443 1113 898 1429
Cuts at: 5417 7129 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1429 1113 898 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2125 2157 7131 7163 2125
Size: 32 4974 32 2133
Fragments arranged by size:
4974 2133 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3443 1316
Size: 2127 5044
XbaI T'CTAG_A
Cuts at: 4507 4507
Size: 7171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2531 3250 4033 2531
Size: 719 783 5669
Fragments arranged by size:
5669 783 719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7171
XmaI C'CCGG_G
Cuts at: 1870 2811 4496 1870
Size: 941 1685 4545
Fragments arranged by size:
4545 1685 941
XmnI GAAnn'nnTTC
Cuts at: 5300 5300
Size: 7171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4979 276
Size: 53 83 186 936 3445 2468
Fragments arranged by size:
3445 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I FseI FspAI KasI NarI NruI
NsiI PacI PspOMI PspXI PsrI RsrII SacII SfoI
SrfI SwaI