Restriction map for
pFN21AE3629
(Circular) (Six-base) MAPSORT of: pFN21AE3629.seq Check: 1564 from: 1 to: 7171
pFN21AE3629 7171 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4981 278
Size: 53 83 186 936 3445 2468
Fragments arranged by size:
3445 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4514 1937
Size: 2577 4594
Acc65I G'GTAC_C
Cuts at: 4492 4492
Size: 7171
AclI AA'CG_TT
Cuts at: 1563 5298 5671 1563
Size: 3735 373 3063
Fragments arranged by size:
3735 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2502 3764 4076 5234 6618 6886 1632
Size: 870 1262 312 1158 1384 268 1917
Fragments arranged by size:
1917 1384 1262 1158 870 312 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6974
AflIII A'CryG_T
Cuts at: 1203 3553 6070 7047 1203
Size: 2350 2517 977 1327
Fragments arranged by size:
2517 2350 1327 977
AgeI A'CCGG_T
Cuts at: 6002 6002
Size: 7171
AhdI GACnn_n'nnGTC
Cuts at: 2183 4057 5900 2183
Size: 1874 1843 3454
Fragments arranged by size:
3454 1874 1843
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7171
AlwNI CAG_nnn'CTG
Cuts at: 2139 3787 4914 6486 2139
Size: 1648 1127 1572 2824
Fragments arranged by size:
2824 1648 1572 1127
ApaI G_GGCC'C
Cuts at: 4286 4286
Size: 7171
ApaLI G'TGCA_C
Cuts at: 5228 6384 5228
Size: 1156 6015
ApoI r'AATT_y
Cuts at: 1493 2080 2091 2286 2432 3371 3708 3742 4480
Size: 587 11 195 146 939 337 34 738
Cuts at: 4480 4706 4858 6967 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 939 738 587 337 226 195
152 146 34 11
AseI AT'TA_AT
Cuts at: 160 1032 2591 4422 5725 160
Size: 872 1559 1831 1303 1606
Fragments arranged by size:
1831 1606 1559 1303 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
AvaI C'yCGr_G
Cuts at: 1870 1943 4496 1870
Size: 73 2553 4545
Fragments arranged by size:
4545 2553 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2916 2949 3551 3584 2916
Size: 33 602 33 6503
Fragments arranged by size:
6503 602 33 33
BamHI G'GATC_C
Cuts at: 4501 4868 4501
Size: 367 6804
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3979 4492 5947 618
Size: 325 217 627 2192 513 1455 1842
Fragments arranged by size:
2192 1842 1455 627 513 325 217
BanII G_rGCy'C
Cuts at: 1391 4113 4286 4490 1391
Size: 2722 173 204 4072
Fragments arranged by size:
4072 2722 204 173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2223 3571 4206 928
Size: 971 324 1348 635 3893
Fragments arranged by size:
3893 1348 971 635 324
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5362 5396 1903
Size: 34 3425 34 3678
Fragments arranged by size:
3678 3425 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2259 3085 3718 5063 6273 2259
Size: 826 633 1345 1210 3157
Fragments arranged by size:
3157 1345 1210 826 633
BclI T'GATC_A
Cuts at: 1548 2302 1548
Size: 754 6417
BfrBI ATG'CAT
Cuts at: 3494 3494
Size: 7171
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5782 136
Size: 107 122 71 631 605 4110 1525
Fragments arranged by size:
4110 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2533 2750 3649 4021 7166 2533
Size: 217 899 372 3145 2538
Fragments arranged by size:
3145 2538 899 372 217
BlpI GC'TnA_GC
Cuts at: 4588 4588
Size: 7171
Bme1580I G_kGCm'C
Cuts at: 1790 4286 5232 6388 1790
Size: 2496 946 1156 2573
Fragments arranged by size:
2573 2496 1156 946
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7171
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4902 5860 442
Size: 416 53 83 399 24 3485 958 1753
Fragments arranged by size:
3485 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2808 4286 5831 1239
Size: 1569 1478 1545 2579
Fragments arranged by size:
2579 1569 1545 1478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5296 6161 6459 5296
Size: 865 298 6008
Fragments arranged by size:
6008 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2361 5834 882
Size: 617 862 3473 2219
Fragments arranged by size:
3473 2219 862 617
BsaAI yAC'GTr
Cuts at: 493 1204 4962 493
Size: 711 3758 2702
Fragments arranged by size:
3758 2702 711
BsaBI GATnn'nnATC
Cuts at: 7165 7165
Size: 7171
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4978 5360 5995 275
Size: 53 83 186 936 3445 382 635 1451
Fragments arranged by size:
3445 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4188 5604 6002 6276 6423 6834 4188
Size: 1416 398 274 147 411 4525
Fragments arranged by size:
4525 1416 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4155 4185 1178
Size: 30 165 30 2752 30 4164
Fragments arranged by size:
4164 2752 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3186 4436 4452 1186
Size: 2000 1250 16 3905
Fragments arranged by size:
3905 2000 1250 16
BseYI C'CCAG_C
Cuts at: 4139 6374 6870 4139
Size: 2235 496 4440
Fragments arranged by size:
4440 2235 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2833 1163
Size: 573 1097 5501
Fragments arranged by size:
5501 1097 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4621 5382 6410 1540
Size: 275 186 2620 761 1028 2301
Fragments arranged by size:
2620 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3451 4113 5232 5317 6388 3451
Size: 662 1119 85 1071 4234
Fragments arranged by size:
4234 1119 1071 662 85
BsmI GAATG_Cn'
Cuts at: 4682 4775 4682
Size: 93 7078
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3451 4113 4286 4490 5232 5317 6388
Size: 399 1661 662 173 204 742 85 1071
Cuts at: 6388 1391
Size: 2174
Fragments arranged by size:
2174 1661 1071 742 662 399 204 173
85
BspEI T'CCGG_A
Cuts at: 4188 6834 4188
Size: 2646 4525
BspHI T'CATG_A
Cuts at: 3601 5060 6790 3601
Size: 1459 1730 3982
Fragments arranged by size:
3982 1730 1459
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2656 4526 844
Size: 1812 1870 3489
Fragments arranged by size:
3489 1870 1812
BsrBI CCG'CTC
Cuts at: 1394 1823 5058 1394
Size: 429 3235 3507
Fragments arranged by size:
3507 3235 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2065 2324 5666 5840 65
Size: 1183 817 259 3342 174 1396
Fragments arranged by size:
3342 1396 1183 817 259 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3795 5815 6002 1700
Size: 110 1985 2020 187 2869
Fragments arranged by size:
2869 2020 1985 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7171
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7171
BssSI C'ACGA_G
Cuts at: 5231 6243 5231
Size: 1012 6159
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7171
BstBI TT'CG_AA
Cuts at: 5975 5975
Size: 7171
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7171
BstYI r'GATC_y
Cuts at: 1972 2533 2750 3649 4006 4021 4501 4868 5253
Size: 561 217 899 357 15 480 367 385
Cuts at: 5253 5270 6722 6808 6820 7166 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 899 561 480 385 367 357
346 217 86 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 2268 2697 2268
Size: 429 6742
BtgI C'CryG_G
Cuts at: 513 1065 2004 4073 513
Size: 552 939 2069 3611
Fragments arranged by size:
3611 2069 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6432
Fragments arranged by size:
6432 683 56
BtsI GCAGTG_nn'
Cuts at: 3967 4257 4290 4687 5481 5501 3967
Size: 290 33 397 794 20 5637
Fragments arranged by size:
5637 794 397 290 33 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7171
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7136
DraI TTT'AAA
Cuts at: 4475 4475
Size: 7171
DraIII CAC_nnn'GTG
Cuts at: 3446 4378 3446
Size: 932 6239
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4024 6178 809
Size: 507 2708 2154 1802
Fragments arranged by size:
2708 2154 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4618 5507 7136
Size: 54 1425 50 275 18 2788 889 1629
Cuts at: 7136 8
Size: 43
Fragments arranged by size:
2788 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4618 1537
Size: 275 2806 4090
Fragments arranged by size:
4090 2806 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2478 3128 3231 3839 5101 1353
Size: 1125 650 103 608 1262 3423
Fragments arranged by size:
3423 1262 1125 650 608 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5758 6132 6278 387
Size: 1064 138 4169 374 146 1280
Fragments arranged by size:
4169 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4111 4111
Size: 7171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2502 2808 3764 4076 4286 5234 5831
Size: 393 870 306 956 312 210 948 597
Cuts at: 5831 6618 6886 1239
Size: 787 268 1524
Fragments arranged by size:
1524 956 948 870 787 597 393 312
306 268 210
EcoO109I rG'GnC_Cy
Cuts at: 4090 4282 4283 4090
Size: 192 1 6978
Fragments arranged by size:
6978 192 1
EcoRI G'AATT_C
Cuts at: 3742 4480 4858 3742
Size: 738 378 6055
Fragments arranged by size:
6055 738 378
EcoRV GAT'ATC
Cuts at: 2704 2704
Size: 7171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2240 3933 4289 2240
Size: 1693 356 5122
Fragments arranged by size:
5122 1693 356
FspI TGC'GCA
Cuts at: 5677 5677
Size: 7171
HaeII r_GCGC'y
Cuts at: 1678 2104 6318 1678
Size: 426 4214 2531
Fragments arranged by size:
4214 2531 426
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2055 2087 2169 2201 2339
Size: 32 135 32 483 32 82 32 138
Cuts at: 2339 2371 2894 2926 2999 3031 3607 3639 3867
Size: 32 523 32 73 32 576 32 228
Cuts at: 3867 3899 4023 4043 4055 4075 4375 4407 5812
Size: 32 124 20 12 20 300 32 1405
Cuts at: 5812 5844 5886 5918 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1405 576 523 483 300 228 138
135 124 82 73 42 32 32 32
32 32 32 32 32 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2815 3187 4183 4515 4761 669
Size: 988 281 877 372 996 332 246 3079
Fragments arranged by size:
3079 996 988 877 372 332 281 246
HindIII A'AGCT_T
Cuts at: 748 2884 748
Size: 2136 5035
HpaI GTT'AAC
Cuts at: 4761 4761
Size: 7171
KpnI G_GTAC'C
Cuts at: 4496 4496
Size: 7171
MfeI C'AATT_G
Cuts at: 3267 4770 3267
Size: 1503 5668
MluI A'CGCG_T
Cuts at: 7047 7047
Size: 7171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3878 6285 6469 1305
Size: 2573 2407 184 2007
Fragments arranged by size:
2573 2407 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5349
Fragments arranged by size:
5349 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3627 4295 5129 5488 5647 518
Size: 648 2461 668 834 359 159 2042
Fragments arranged by size:
2461 2042 834 668 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3059 3787 4358 4587 5264 6412 6657 1606
Size: 1453 728 571 229 677 1148 245 2120
Fragments arranged by size:
2120 1453 1148 728 677 571 245 229
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5680
Fragments arranged by size:
5680 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7171
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7171
NotI GC'GGCC_GC
Cuts at: 4618 4618
Size: 7171
NsiI A_TGCA'T
Cuts at: 3496 3496
Size: 7171
NspI r_CATG'y
Cuts at: 1132 1229 2311 3498 3557 3932 4343 4529 6074
Size: 97 1082 1187 59 375 411 186 1545
Cuts at: 6074 1132
Size: 2229
Fragments arranged by size:
2229 1545 1187 1082 411 375 186 97
59
PasI CC'CwG_GG
Cuts at: 1348 4279 1348
Size: 2931 4240
PciI A'CATG_T
Cuts at: 3553 6070 3553
Size: 2517 4654
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2149 3666 1108
Size: 1041 1517 4613
Fragments arranged by size:
4613 1517 1041
PmeI GTTT'AAAC
Cuts at: 4475 4475
Size: 7171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5240 5272 6753 6785 5240
Size: 32 1481 32 5626
Fragments arranged by size:
5626 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4090 4090
Size: 7171
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7171
PsiI TTA'TAA
Cuts at: 2507 4741 2507
Size: 2234 4937
PspOMI G'GGCC_C
Cuts at: 4282 4282
Size: 7171
PstI C_TGCA'G
Cuts at: 830 3058 4264 4523 830
Size: 2228 1206 259 3478
Fragments arranged by size:
3478 2228 1206 259
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7171
PvuII CAG'CTG
Cuts at: 3059 3787 4358 3059
Size: 728 571 5872
Fragments arranged by size:
5872 728 571
SacI G_AGCT'C
Cuts at: 4113 4113
Size: 7171
SalI G'TCGA_C
Cuts at: 1936 4513 1936
Size: 2577 4594
SapI GCTCTTCn'nnn_
Cuts at: 2478 3231 2478
Size: 753 6418
SbfI CC_TGCA'GG
Cuts at: 4523 4523
Size: 7171
ScaI AGT'ACT
Cuts at: 5419 7131 5419
Size: 1712 5459
SfcI C'TryA_G
Cuts at: 826 1045 3054 3291 4260 4519 5654 6335 6526
Size: 219 2009 237 969 259 1135 681 191
Cuts at: 6526 826
Size: 1471
Fragments arranged by size:
2009 1471 1135 969 681 259 237 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7171
SmaI CCC'GGG
Cuts at: 1872 4498 1872
Size: 2626 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5275 6176 6438 820
Size: 197 926 3332 901 262 1553
Fragments arranged by size:
3332 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4962 493
Size: 4469 2702
SpeI A'CTAG_T
Cuts at: 152 2179 152
Size: 2027 5144
SphI G_CATG'C
Cuts at: 1132 3932 4529 1132
Size: 2800 597 3774
Fragments arranged by size:
3774 2800 597
SspI AAT'ATT
Cuts at: 5 52 5095 5
Size: 47 5043 2081
Fragments arranged by size:
5043 2081 47
StuI AGG'CCT
Cuts at: 1517 4443 1517
Size: 2926 4245
StyI C'CwwG_G
Cuts at: 513 1065 2004 3483 3665 4610 513
Size: 552 939 1479 182 945 3074
Fragments arranged by size:
3074 1479 945 939 552 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2141 4153 5198 5215 5368 5553 5998
Size: 1255 561 2012 1045 17 153 185 445
Cuts at: 5998 325
Size: 1498
Fragments arranged by size:
2012 1498 1255 1045 561 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5417 7129 96
Size: 274 80 33 51 1443 3440 1712 138
Fragments arranged by size:
3440 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3271 3303 7131 7163 3271
Size: 32 3828 32 3279
Fragments arranged by size:
3828 3279 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2895 2938 1316
Size: 1579 43 5549
Fragments arranged by size:
5549 1579 43
XbaI T'CTAG_A
Cuts at: 4408 4507 4408
Size: 99 7072
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7171
XmaI C'CCGG_G
Cuts at: 1870 4496 1870
Size: 2626 4545
XmnI GAAnn'nnTTC
Cuts at: 2826 5300 2826
Size: 2474 4697
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4979 276
Size: 53 83 186 936 3445 2468
Fragments arranged by size:
3445 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI