Restriction map for
pFN21AE3618
(Circular) (Six-base) MAPSORT of: pFN21AE3618.seq Check: 8391 from: 1 to: 5599
pFN21AE3618 5599 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2144 2144
Size: 5599
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3409 278
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2942 1937
Size: 1005 4594
Acc65I G'GTAC_C
Cuts at: 2595 2920 2595
Size: 325 5274
AclI AA'CG_TT
Cuts at: 1563 3726 4099 1563
Size: 2163 373 3063
Fragments arranged by size:
3063 2163 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3662 5046 5314 1632
Size: 2030 1384 268 1917
Fragments arranged by size:
2030 1917 1384 268
AfeI AGC'GCT
Cuts at: 2090 2090
Size: 5599
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5402
AflIII A'CryG_T
Cuts at: 1203 4498 5475 1203
Size: 3295 977 1327
Fragments arranged by size:
3295 1327 977
AgeI A'CCGG_T
Cuts at: 4430 4430
Size: 5599
AhdI GACnn_n'nnGTC
Cuts at: 4328 4328
Size: 5599
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5599
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2897 1184
Size: 1713 3886
AlwNI CAG_nnn'CTG
Cuts at: 2145 2170 2464 2524 2567 3342 4914 2145
Size: 25 294 60 43 775 1572 2830
Fragments arranged by size:
2830 1572 775 294 60 43 25
ApaLI G'TGCA_C
Cuts at: 3656 4812 3656
Size: 1156 4443
ApoI r'AATT_y
Cuts at: 1493 2908 3134 3286 5395 1493
Size: 1415 226 152 2109 1697
Fragments arranged by size:
2109 1697 1415 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4153 160
Size: 872 3121 1606
Fragments arranged by size:
3121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
AvaI C'yCGr_G
Cuts at: 1870 1943 2318 2924 1870
Size: 73 375 606 4545
Fragments arranged by size:
4545 606 375 73
AvrII C'CTAG_G
Cuts at: 2122 2122
Size: 5599
BamHI G'GATC_C
Cuts at: 2929 3296 2929
Size: 367 5232
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2213 2311 2595 2920 4375
Size: 325 217 627 426 98 284 325 1455
Cuts at: 4375 618
Size: 1842
Fragments arranged by size:
1842 1455 627 426 325 325 284 217
98
BanII G_rGCy'C
Cuts at: 1391 2261 2531 2703 2866 2918 1391
Size: 870 270 172 163 52 4072
Fragments arranged by size:
4072 870 270 172 163 52
BbeI G_GCGC'C
Cuts at: 2217 2217
Size: 5599
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2562 928
Size: 971 663 3965
Fragments arranged by size:
3965 971 663
BbvCI CC'TCA_GC
Cuts at: 2307 2307
Size: 5599
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3790 3824 1903
Size: 34 1853 34 3678
Fragments arranged by size:
3678 1853 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3491 4701 3491
Size: 1210 4389
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5599
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2570 2681 4210
Size: 107 122 71 631 605 898 111 1529
Cuts at: 4210 136
Size: 1525
Fragments arranged by size:
1529 1525 898 631 605 122 111 107
71
BglII A'GATC_T
Cuts at: 5594 5594
Size: 5599
BlpI GC'TnA_GC
Cuts at: 3016 3016
Size: 5599
Bme1580I G_kGCm'C
Cuts at: 1790 2644 2833 2852 3660 4816 1790
Size: 854 189 19 808 1156 2573
Fragments arranged by size:
2573 1156 854 808 189 19
BmgBI CAC'GTC
Cuts at: 1318 2757 1318
Size: 1439 4160
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2068 3330 4288
Size: 416 53 83 399 24 651 1262 958
Cuts at: 4288 442
Size: 1753
Fragments arranged by size:
1753 1262 958 651 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5599
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 5599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4259 1239
Size: 3020 2579
Bpu10I CC'TnA_GC
Cuts at: 2307 2601 2307
Size: 294 5305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2717 3724 4589 4887 2717
Size: 1007 865 298 3429
Fragments arranged by size:
3429 1007 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4262 882
Size: 617 2763 2219
Fragments arranged by size:
2763 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2155 3390 493
Size: 711 951 1235 2702
Fragments arranged by size:
2702 1235 951 711
BsaBI GATnn'nnATC
Cuts at: 5593 5593
Size: 5599
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2214 3406 3788 4423
Size: 53 83 186 936 681 1192 382 635
Cuts at: 4423 275
Size: 1451
Fragments arranged by size:
1451 1192 936 681 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2469 2551 4032 4430 4704 4851 5262 2469
Size: 82 1481 398 274 147 411 2806
Fragments arranged by size:
2806 1481 411 398 274 147 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2741 2771 1178
Size: 30 165 30 1338 30 4006
Fragments arranged by size:
4006 1338 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2219 2222 2279 2762 1186
Size: 1024 9 3 57 483 4023
Fragments arranged by size:
4023 1024 483 57 9 3
BseYI C'CCAG_C
Cuts at: 2450 2517 2524 4802 5298 2450
Size: 67 7 2278 496 2751
Fragments arranged by size:
2751 2278 496 67 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2018 2522 1163
Size: 573 282 504 4240
Fragments arranged by size:
4240 573 504 282
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3049 3810 4838 1540
Size: 275 186 1048 761 1028 2301
Fragments arranged by size:
2301 1048 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3660 3745 4816 3660
Size: 85 1071 4443
Fragments arranged by size:
4443 1071 85
BsmI GAATG_Cn'
Cuts at: 3110 3203 3110
Size: 93 5506
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2261 2531 2644 2703 2833 2852 2866
Size: 399 471 270 113 59 130 19 14
Cuts at: 2866 2918 3660 3745 4816 1391
Size: 52 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 471 399 270 130 113
85 59 52 19 14
BspEI T'CCGG_A
Cuts at: 5262 5262
Size: 5599
BspHI T'CATG_A
Cuts at: 3488 5218 3488
Size: 1730 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2144 2954 844
Size: 1300 810 3489
Fragments arranged by size:
3489 1300 810
BsrBI CCG'CTC
Cuts at: 1394 1823 2103 3486 1394
Size: 429 280 1383 3507
Fragments arranged by size:
3507 1383 429 280
BsrDI GCAATG_nn'
Cuts at: 65 1248 4094 4268 65
Size: 1183 2846 174 1396
Fragments arranged by size:
2846 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2066 2336 4243 4430 1700
Size: 110 256 270 1907 187 2869
Fragments arranged by size:
2869 1907 270 256 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5599
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5599
BssSI C'ACGA_G
Cuts at: 3659 4671 3659
Size: 1012 4587
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2766 794
Size: 1972 3627
BstBI TT'CG_AA
Cuts at: 4403 4403
Size: 5599
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5599
BstXI CCAn_nnnn'nTGG
Cuts at: 2159 2719 2159
Size: 560 5039
BstYI r'GATC_y
Cuts at: 1972 2166 2929 3296 3681 3698 5150 5236 5248
Size: 194 763 367 385 17 1452 86 12
Cuts at: 5248 5594 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 763 385 367 346 194 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2038 2540 513
Size: 552 939 34 502 3572
Fragments arranged by size:
3572 939 552 502 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4860
Fragments arranged by size:
4860 683 56
BtsI GCAGTG_nn'
Cuts at: 3115 3909 3929 3115
Size: 794 20 4785
Fragments arranged by size:
4785 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5564
DraI TTT'AAA
Cuts at: 2903 2903
Size: 5599
DraIII CAC_nnn'GTG
Cuts at: 2500 2500
Size: 5599
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4606 809
Size: 507 3290 1802
Fragments arranged by size:
3290 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2294 2806 3046
Size: 54 1425 50 275 18 464 512 240
Cuts at: 3046 3935 5564 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 512 464 275 240 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3046 1537
Size: 275 1234 4090
Fragments arranged by size:
4090 1234 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2196 2476 3529 1353
Size: 843 280 1053 3423
Fragments arranged by size:
3423 1053 843 280
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4186 4560 4706 387
Size: 1064 138 2597 374 146 1280
Fragments arranged by size:
2597 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3662 4259 5046 5314 1239
Size: 393 2030 597 787 268 1524
Fragments arranged by size:
2030 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2121 2192 2230 2121
Size: 71 38 5490
Fragments arranged by size:
5490 71 38
EcoO109I rG'GnC_Cy
Cuts at: 2009 2237 2392 2536 2009
Size: 228 155 144 5072
Fragments arranged by size:
5072 228 155 144
EcoRI G'AATT_C
Cuts at: 2908 3286 2908
Size: 378 5221
FspI TGC'GCA
Cuts at: 2885 4105 2885
Size: 1220 4379
FspAI rTGC'GCAy
Cuts at: 2885 2885
Size: 5599
HaeII r_GCGC'y
Cuts at: 1678 2092 2217 4746 1678
Size: 414 125 2529 2531
Fragments arranged by size:
2531 2529 414 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2475 2507 2615 2647 2853
Size: 32 135 32 903 32 108 32 206
Cuts at: 2853 2885 4240 4272 4314 4346 1373
Size: 32 1355 32 42 32 2626
Fragments arranged by size:
2626 1355 903 206 135 108 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2943 3189 669
Size: 988 281 1005 246 3079
Fragments arranged by size:
3079 1005 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5599
HpaI GTT'AAC
Cuts at: 3189 3189
Size: 5599
KasI G'GCGC_C
Cuts at: 2213 2213
Size: 5599
KpnI G_GTAC'C
Cuts at: 2599 2924 2599
Size: 325 5274
MfeI C'AATT_G
Cuts at: 3198 3198
Size: 5599
MluI A'CGCG_T
Cuts at: 5475 5475
Size: 5599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4713 4897 1305
Size: 3408 184 2007
Fragments arranged by size:
3408 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3777
Fragments arranged by size:
3777 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2157 3557 3916 4075 518
Size: 648 991 1400 359 159 2042
Fragments arranged by size:
2042 1400 991 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2179 2299 2454 2521 2731 2845 3015 3692
Size: 573 120 155 67 210 114 170 677
Cuts at: 3692 4840 5085 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 573 245 210 170 155
120 114 67
NaeI GCC'GGC
Cuts at: 2338 2338
Size: 5599
NarI GG'CG_CC
Cuts at: 2214 2214
Size: 5599
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4108
Fragments arranged by size:
4108 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5599
NgoMIV G'CCGG_C
Cuts at: 2336 2336
Size: 5599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5599
NotI GC'GGCC_GC
Cuts at: 3046 3046
Size: 5599
NspI r_CATG'y
Cuts at: 1132 1229 2438 2957 4502 1132
Size: 97 1209 519 1545 2229
Fragments arranged by size:
2229 1545 1209 519 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5599
PciI A'CATG_T
Cuts at: 4498 4498
Size: 5599
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2524 1108
Size: 1416 4183
PmeI GTTT'AAAC
Cuts at: 2903 2903
Size: 5599
PmlI CAC'GTG
Cuts at: 2155 2155
Size: 5599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 5181 5213 3668
Size: 32 1481 32 4054
Fragments arranged by size:
4054 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2009 2536 2009
Size: 527 5072
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5599
PsiI TTA'TAA
Cuts at: 3169 3169
Size: 5599
PstI C_TGCA'G
Cuts at: 830 2141 2400 2745 2844 2861 2951 830
Size: 1311 259 345 99 17 90 3478
Fragments arranged by size:
3478 1311 345 259 99 90 17
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5599
PvuII CAG'CTG
Cuts at: 2179 2454 2521 2731 2845 2179
Size: 275 67 210 114 4933
Fragments arranged by size:
4933 275 210 114 67
SalI G'TCGA_C
Cuts at: 1936 2941 1936
Size: 1005 4594
SanDI GG'GwC_CC
Cuts at: 2536 2536
Size: 5599
SbfI CC_TGCA'GG
Cuts at: 2951 2951
Size: 5599
ScaI AGT'ACT
Cuts at: 3847 5559 3847
Size: 1712 3887
SexAI A'CCwGG_T
Cuts at: 2811 2811
Size: 5599
SfcI C'TryA_G
Cuts at: 826 1045 2137 2396 2741 2840 2857 2947 4082
Size: 219 1092 259 345 99 17 90 1135
Cuts at: 4082 4763 4954 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1092 681 345 259 219 191
99 90 17
SfoI GGC'GCC
Cuts at: 2215 2215
Size: 5599
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5599
SmaI CCC'GGG
Cuts at: 1872 2926 1872
Size: 1054 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2696 3703 4604 4866 820
Size: 197 926 753 1007 901 262 1553
Fragments arranged by size:
1553 1007 926 901 753 262 197
SnaBI TAC'GTA
Cuts at: 493 3390 493
Size: 2897 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5599
SphI G_CATG'C
Cuts at: 1132 2438 2957 1132
Size: 1306 519 3774
Fragments arranged by size:
3774 1306 519
SspI AAT'ATT
Cuts at: 5 52 3523 5
Size: 47 3471 2081
Fragments arranged by size:
3471 2081 47
StuI AGG'CCT
Cuts at: 1517 2127 1517
Size: 610 4989
StyI C'CwwG_G
Cuts at: 513 1065 2004 2122 3038 513
Size: 552 939 118 916 3074
Fragments arranged by size:
3074 939 916 552 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2629 3626 3643 3796 3981 4426 325
Size: 1255 1049 997 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1049 997 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3845 5557 96
Size: 274 80 33 51 1443 1868 1712 138
Fragments arranged by size:
1868 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2741 2773 5559 5591 2741
Size: 32 2786 32 2749
Fragments arranged by size:
2786 2749 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2489 1316
Size: 1173 4426
XbaI T'CTAG_A
Cuts at: 2935 2935
Size: 5599
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5599
XmaI C'CCGG_G
Cuts at: 1870 2924 1870
Size: 1054 4545
XmnI GAAnn'nnTTC
Cuts at: 3728 3728
Size: 5599
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3407 276
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I EcoICRI EcoRV FalI FseI NruI NsiI PacI
PfoI PspOMI PspXI PsrI RsrII SacI SacII SapI
SfiI SrfI SwaI XcmI