Restriction map for
pFN21AE3615
(Circular) (Six-base) MAPSORT of: pFN21AE3615.seq Check: 2671 from: 1 to: 5725
pFN21AE3615 5725 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3068 1937
Size: 1131 4594
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3024 3852 4225 1563
Size: 1461 828 373 3063
Fragments arranged by size:
3063 1461 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3788 5172 5440 1632
Size: 2156 1384 268 1917
Fragments arranged by size:
2156 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 2361 2498 4624 5601 1203
Size: 1158 137 2126 977 1327
Fragments arranged by size:
2126 1327 1158 977 137
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 2658 4454 2658
Size: 1796 3929
AleI CACnn'nnGTG
Cuts at: 1166 2556 2828 1166
Size: 1390 272 4063
Fragments arranged by size:
4063 1390 272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5725
AlwNI CAG_nnn'CTG
Cuts at: 2785 3468 5040 2785
Size: 683 1572 3470
Fragments arranged by size:
3470 1572 683
ApaLI G'TGCA_C
Cuts at: 2273 3782 4938 2273
Size: 1509 1156 3060
Fragments arranged by size:
3060 1509 1156
ApoI r'AATT_y
Cuts at: 1493 3034 3260 3412 5521 1493
Size: 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 2485 3050 1870
Size: 73 542 565 4545
Fragments arranged by size:
4545 565 542 73
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3046 4501 618
Size: 325 217 627 1259 1455 1842
Fragments arranged by size:
1842 1455 1259 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2738 3044 1391
Size: 1347 306 4072
Fragments arranged by size:
4072 1347 306
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2540 928
Size: 971 641 4113
Fragments arranged by size:
4113 971 641
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3916 3950 1903
Size: 34 1979 34 3678
Fragments arranged by size:
3678 1979 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3617 4827 3617
Size: 1210 4515
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4336 136
Size: 107 122 71 631 605 2664 1525
Fragments arranged by size:
2664 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5720 5720
Size: 5725
BlpI GC'TnA_GC
Cuts at: 3142 3142
Size: 5725
Bme1580I G_kGCm'C
Cuts at: 1790 2277 3786 4942 1790
Size: 487 1509 1156 2573
Fragments arranged by size:
2573 1509 1156 487
BmgBI CAC'GTC
Cuts at: 1318 2826 1318
Size: 1508 4217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2200 2532 3456
Size: 416 53 83 399 24 783 332 924
Cuts at: 3456 4414 442
Size: 958 1753
Fragments arranged by size:
1753 958 924 783 416 399 332 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4385 1239
Size: 3146 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 3850 4715 5013 2030
Size: 1820 865 298 2742
Fragments arranged by size:
2742 1820 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4388 882
Size: 617 2889 2219
Fragments arranged by size:
2889 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2499 3516 493
Size: 711 1295 1017 2702
Fragments arranged by size:
2702 1295 1017 711
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3532 3914 4549 275
Size: 53 83 186 936 1999 382 635 1451
Fragments arranged by size:
1999 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4158 4556 4830 4977 5388 4158
Size: 398 274 147 411 4495
Fragments arranged by size:
4495 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5500
Fragments arranged by size:
5500 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2284 1186
Size: 1098 4627
BseYI C'CCAG_C
Cuts at: 4928 5424 4928
Size: 496 5229
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2713 1163
Size: 573 977 4175
Fragments arranged by size:
4175 977 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3175 3936 4964 1540
Size: 275 186 1174 761 1028 2301
Fragments arranged by size:
2301 1174 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 3786 3871 4942 2277
Size: 1509 85 1071 3060
Fragments arranged by size:
3060 1509 1071 85
BsmI GAATG_Cn'
Cuts at: 3236 3329 3236
Size: 93 5632
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2738 3044 3786 3871 4942 1391
Size: 399 487 461 306 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 487 461 399 306 85
BspEI T'CCGG_A
Cuts at: 5388 5388
Size: 5725
BspHI T'CATG_A
Cuts at: 2304 3614 5344 2304
Size: 1310 1730 2685
Fragments arranged by size:
2685 1730 1310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2663 2775 3080 844
Size: 1819 112 305 3489
Fragments arranged by size:
3489 1819 305 112
BsrBI CCG'CTC
Cuts at: 1394 1823 3612 1394
Size: 429 1789 3507
Fragments arranged by size:
3507 1789 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2404 2893 4220 4394 65
Size: 1183 1156 489 1327 174 1396
Fragments arranged by size:
1396 1327 1183 1156 489 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4369 4556 1700
Size: 110 2559 187 2869
Fragments arranged by size:
2869 2559 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 2678 2801 3785 4797 2678
Size: 123 984 1012 3606
Fragments arranged by size:
3606 1012 984 123
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2814 794
Size: 2020 3705
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5725
BstYI r'GATC_y
Cuts at: 1972 3055 3422 3807 3824 5276 5362 5374 5720
Size: 1083 367 385 17 1452 86 12 346
Cuts at: 5720 1972
Size: 1977
Fragments arranged by size:
1977 1452 1083 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2829 513
Size: 552 939 825 3409
Fragments arranged by size:
3409 939 825 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 2285 3241 4035 4055 2285
Size: 956 794 20 3955
Fragments arranged by size:
3955 956 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5690
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DraIII CAC_nnn'GTG
Cuts at: 2499 2499
Size: 5725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4732 809
Size: 507 3416 1802
Fragments arranged by size:
3416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2169 2674 2848
Size: 54 1425 50 275 18 339 505 174
Cuts at: 2848 2932 2985 3172 4061 5690 8
Size: 84 53 187 889 1629 43
Fragments arranged by size:
1629 1425 889 505 339 275 187 174
84 54 53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3172 1537
Size: 275 1360 4090
Fragments arranged by size:
4090 1360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2603 3655 1353
Size: 1250 1052 3423
Fragments arranged by size:
3423 1250 1052
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2164 4312 4686 4832 387
Size: 1064 138 575 2148 374 146 1280
Fragments arranged by size:
2148 1280 1064 575 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3788 4385 5172 5440 1239
Size: 393 2156 597 787 268 1524
Fragments arranged by size:
2156 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2610 2652 2610
Size: 42 5683
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 4872 1678
Size: 3194 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2644 2676 2709
Size: 32 135 32 518 32 522 32 33
Cuts at: 2709 2741 4366 4398 4440 4472 1373
Size: 32 1625 32 42 32 2626
Fragments arranged by size:
2626 1625 522 518 135 42 33 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3069 3315 669
Size: 988 281 1131 246 3079
Fragments arranged by size:
3079 1131 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2664 4839 5023 1305
Size: 1359 2175 184 2007
Fragments arranged by size:
2175 2007 1359 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2171 2676 2934 2987 10
Size: 54 1425 343 339 505 258 53 2748
Fragments arranged by size:
2748 1425 505 343 339 258 54 53
MslI CAynn'nnrTG
Cuts at: 518 1166 2556 2828 3683 4042 4201 518
Size: 648 1390 272 855 359 159 2042
Fragments arranged by size:
2042 1390 855 648 359 272 159
MspA1I CmG'CkG
Cuts at: 1606 2969 3141 3818 4966 5211 1606
Size: 1363 172 677 1148 245 2120
Fragments arranged by size:
2120 1363 1148 677 245 172
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4234
Fragments arranged by size:
4234 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 2346 2365 3083 4628 1132
Size: 97 1117 19 718 1545 2229
Fragments arranged by size:
2229 1545 1117 718 97 19
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 2361 4624 2361
Size: 2263 3462
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2190 2634 1108
Size: 1082 444 4199
Fragments arranged by size:
4199 1082 444
PfoI T'CCnGG_A
Cuts at: 2457 2457
Size: 5725
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PmlI CAC'GTG
Cuts at: 2499 2499
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2652 2652
Size: 5725
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PstI C_TGCA'G
Cuts at: 830 2819 3077 830
Size: 1989 258 3478
Fragments arranged by size:
3478 1989 258
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
PvuII CAG'CTG
Cuts at: 2969 2969
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SbfI CC_TGCA'GG
Cuts at: 3077 3077
Size: 5725
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SfcI C'TryA_G
Cuts at: 826 1045 2815 3073 4208 4889 5080 826
Size: 219 1770 258 1135 681 191 1471
Fragments arranged by size:
1770 1471 1135 681 258 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 3052 1872
Size: 1180 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 3829 4730 4992 820
Size: 197 926 102 1784 901 262 1553
Fragments arranged by size:
1784 1553 926 901 262 197 102
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 3083 1132
Size: 1951 3774
SspI AAT'ATT
Cuts at: 5 52 2473 3649 5
Size: 47 2421 1176 2081
Fragments arranged by size:
2421 2081 1176 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5725
StyI C'CwwG_G
Cuts at: 513 1065 2004 3164 513
Size: 552 939 1160 3074
Fragments arranged by size:
3074 1160 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3752 3769 3922 4107 4552 325
Size: 1255 2172 17 153 185 445 1498
Fragments arranged by size:
2172 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3971 5683 96
Size: 274 80 33 51 1443 1994 1712 138
Fragments arranged by size:
1994 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2062 2094 2348 2380 2483 2515 5685 5717 2062
Size: 32 254 32 103 32 3170 32 2070
Fragments arranged by size:
3170 2070 254 103 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5725
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 3050 1870
Size: 1180 4545
XmnI GAAnn'nnTTC
Cuts at: 3854 3854
Size: 5725
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI