Restriction map for
pFN21AE3608
(Circular) (Six-base) MAPSORT of: pFN21AE3608.seq Check: 7852 from: 1 to: 8701
pFN21AE3608 8701 bp
With 182 enzymes: SgfI *
March 28, 2012 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3180 3180
Size: 8701
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4617 6511 278
Size: 53 83 186 936 3081 1894 2468
Fragments arranged by size:
3081 2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 5505 5580 5709 6044 1937
Size: 1113 2455 75 129 335 4594
Fragments arranged by size:
4594 2455 1113 335 129 75
Acc65I G'GTAC_C
Cuts at: 6022 6022
Size: 8701
AclI AA'CG_TT
Cuts at: 1563 6828 7201 1563
Size: 5265 373 3063
Fragments arranged by size:
5265 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 2876 3452 3791 3960 4701 6764 8148
Size: 767 477 576 339 169 741 2063 1384
Cuts at: 8148 8416 1632
Size: 268 1917
Fragments arranged by size:
2063 1917 1384 767 741 576 477 339
268 169
AflII C'TTAA_G
Cuts at: 820 1017 2909 820
Size: 197 1892 6612
Fragments arranged by size:
6612 1892 197
AflIII A'CryG_T
Cuts at: 1203 2807 5718 7600 8577 1203
Size: 1604 2911 1882 977 1327
Fragments arranged by size:
2911 1882 1604 1327 977
AgeI A'CCGG_T
Cuts at: 7532 7532
Size: 8701
AhdI GACnn_n'nnGTC
Cuts at: 4474 4816 7430 4474
Size: 342 2614 5745
Fragments arranged by size:
5745 2614 342
AleI CACnn'nnGTG
Cuts at: 1166 4601 4661 5361 1166
Size: 3435 60 700 4506
Fragments arranged by size:
4506 3435 700 60
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2172 3438 5696 1184
Size: 988 1266 2258 4189
Fragments arranged by size:
4189 2258 1266 988
AlwNI CAG_nnn'CTG
Cuts at: 2345 2854 3127 3310 3493 3788 3808 6444 8016
Size: 509 273 183 183 295 20 2636 1572
Cuts at: 8016 2345
Size: 3030
Fragments arranged by size:
3030 2636 1572 509 295 273 183 183
20
ApaI G_GGCC'C
Cuts at: 3565 3678 4167 5017 5437 3565
Size: 113 489 850 420 6829
Fragments arranged by size:
6829 850 489 420 113
ApaLI G'TGCA_C
Cuts at: 5615 6758 7914 5615
Size: 1143 1156 6402
Fragments arranged by size:
6402 1156 1143
ApoI r'AATT_y
Cuts at: 1493 3922 5453 6010 6236 6388 8497 1493
Size: 2429 1531 557 226 152 2109 1697
Fragments arranged by size:
2429 2109 1697 1531 557 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7255 160
Size: 872 6223 1606
Fragments arranged by size:
6223 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8701
AvaI C'yCGr_G
Cuts at: 1870 1943 2287 3152 3564 3900 5010 5584 6026
Size: 73 344 865 412 336 1110 574 442
Cuts at: 6026 1870
Size: 4545
Fragments arranged by size:
4545 1110 865 574 442 412 344 336
73
BamHI G'GATC_C
Cuts at: 6031 6398 6031
Size: 367 8334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3219 4006 4145 4272 4655
Size: 325 217 627 1432 787 139 127 383
Cuts at: 4655 6022 7477 618
Size: 1367 1455 1842
Fragments arranged by size:
1842 1455 1432 1367 787 627 383 325
217 139 127
BanII G_rGCy'C
Cuts at: 1391 2285 2695 2728 3565 3651 3678 3726 4167
Size: 894 410 33 837 86 27 48 441
Cuts at: 4167 4853 5017 5211 5414 5437 5884 6020 1391
Size: 686 164 194 203 23 447 136 4072
Fragments arranged by size:
4072 894 837 686 447 441 410 203
194 164 136 86 48 33 27 23
BbeI G_GCGC'C
Cuts at: 3223 3223
Size: 8701
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5857 928
Size: 971 3958 3772
Fragments arranged by size:
3958 3772 971
BbvCI CC'TCA_GC
Cuts at: 2499 2499
Size: 8701
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4930 4964 6892 6926 1903
Size: 34 2993 34 1928 34 3678
Fragments arranged by size:
3678 2993 1928 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4573 6593 7803 4573
Size: 2020 1210 5471
Fragments arranged by size:
5471 2020 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8701
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4162 4396 7312
Size: 107 122 71 631 605 2490 234 2916
Cuts at: 7312 136
Size: 1525
Fragments arranged by size:
2916 2490 1525 631 605 234 122 107
71
BglII A'GATC_T
Cuts at: 8696 8696
Size: 8701
BlpI GC'TnA_GC
Cuts at: 2532 4173 6118 2532
Size: 1641 1945 5115
Fragments arranged by size:
5115 1945 1641
Bme1580I G_kGCm'C
Cuts at: 1790 3565 3678 3901 4148 4167 4275 4943 5017
Size: 1775 113 223 247 19 108 668 74
Cuts at: 5017 5111 5358 5437 5619 6762 7918 1790
Size: 94 247 79 182 1143 1156 2573
Fragments arranged by size:
2573 1775 1156 1143 668 247 247 223
182 113 108 94 79 74 19
BmgBI CAC'GTC
Cuts at: 1318 4738 4969 1318
Size: 3420 231 5050
Fragments arranged by size:
5050 3420 231
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3796 3888 6007
Size: 416 53 83 399 24 2379 92 2119
Cuts at: 6007 6432 7390 442
Size: 425 958 1753
Fragments arranged by size:
2379 2119 1753 958 425 416 399 92
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8701
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3040 5851 3040
Size: 2811 5890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2577 3062 3063 3314 3383 3392 4143 4316
Size: 1338 485 1 251 69 9 751 173
Cuts at: 4316 4911 5222 5536 7361 1239
Size: 595 311 314 1825 2579
Fragments arranged by size:
2579 1825 1338 751 595 485 314 311
251 173 69 9 1
Bpu10I CC'TnA_GC
Cuts at: 2499 2828 3645 2499
Size: 329 817 7555
Fragments arranged by size:
7555 817 329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2747 3416 5390 5519 5876 6826 7691 7989 2747
Size: 669 1974 129 357 950 865 298 3459
Fragments arranged by size:
3459 1974 950 865 669 357 298 129
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2661 3141 5018 7364 882
Size: 617 1162 480 1877 2346 2219
Fragments arranged by size:
2346 2219 1877 1162 617 480
BsaAI yAC'GTr
Cuts at: 493 1204 5721 6492 493
Size: 711 4517 771 2702
Fragments arranged by size:
4517 2702 771 711
BsaBI GATnn'nnATC
Cuts at: 8695 8695
Size: 8701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3220 4614 6508 6890
Size: 53 83 186 936 1687 1394 1894 382
Cuts at: 6890 7525 275
Size: 635 1451
Fragments arranged by size:
1894 1687 1451 1394 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5037 5274 7134 7532 7806 7953 8364 5037
Size: 237 1860 398 274 147 411 5374
Fragments arranged by size:
5374 1860 411 398 274 237 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5774 5804 1178
Size: 30 165 30 4371 30 4075
Fragments arranged by size:
4371 4075 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 2969 2999 3215 3227 3245 3335 3338
Size: 1018 765 30 216 12 18 90 3
Cuts at: 3338 3389 3690 3918 4234 4309 4502 4529 4634
Size: 51 301 228 316 75 193 27 105
Cuts at: 4634 4700 5534 1186
Size: 66 834 4353
Fragments arranged by size:
4353 1018 834 765 316 301 228 216
193 105 90 75 66 51 30 27
18 12 3
BseYI C'CCAG_C
Cuts at: 2014 2069 3844 3953 3967 4233 4635 4748 5808
Size: 55 1775 109 14 266 402 113 1060
Cuts at: 5808 7904 8400 2014
Size: 2096 496 2315
Fragments arranged by size:
2315 2096 1775 1060 496 402 266 113
109 55 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3161 5097 5273 1163
Size: 573 1425 1936 176 4591
Fragments arranged by size:
4591 1936 1425 573 176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6151 6912 7940 1540
Size: 275 186 4150 761 1028 2301
Fragments arranged by size:
4150 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2533 4347 4853 5414 5619 5884 6762 6847 7918
Size: 1814 506 561 205 265 878 85 1071
Cuts at: 7918 2533
Size: 3316
Fragments arranged by size:
3316 1814 1071 878 561 506 265 205
85
BsiWI C'GTAC_G
Cuts at: 5721 5721
Size: 8701
BsmI GAATG_Cn'
Cuts at: 2500 6212 6305 2500
Size: 3712 93 4896
Fragments arranged by size:
4896 3712 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3401 5837 3401
Size: 2436 6265
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2285 2533 2695 2728 3565 3651 3678
Size: 399 495 248 162 33 837 86 27
Cuts at: 3678 3726 3901 4148 4167 4275 4347 4853 4943
Size: 48 175 247 19 108 72 506 90
Cuts at: 4943 5017 5111 5211 5358 5414 5437 5619 5884
Size: 74 94 100 147 56 23 182 265
Cuts at: 5884 6020 6762 6847 7918 1391
Size: 136 742 85 1071 2174
Fragments arranged by size:
2174 1071 837 742 506 495 399 265
248 247 182 175 162 147 136 108
100 94 90 86 85 74 72 56
48 33 27 23 19
BspEI T'CCGG_A
Cuts at: 5037 5274 8364 5037
Size: 237 3090 5374
Fragments arranged by size:
5374 3090 237
BspHI T'CATG_A
Cuts at: 3030 5967 5991 6590 8320 3030
Size: 2937 24 599 1730 3411
Fragments arranged by size:
3411 2937 1730 599 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2884 3180 4704 4737 5106 5223 6056 844
Size: 2040 296 1524 33 369 117 833 3489
Fragments arranged by size:
3489 2040 1524 833 369 296 117 33
BsrBI CCG'CTC
Cuts at: 1394 1823 2890 3217 3250 3328 4141 4300 4383
Size: 429 1067 327 33 78 813 159 83
Cuts at: 4383 4426 4690 5380 5835 5908 5940 6588 1394
Size: 43 264 690 455 73 32 648 3507
Fragments arranged by size:
3507 1067 813 690 648 455 429 327
264 159 83 78 73 43 33 32
BsrDI GCAATG_nn'
Cuts at: 65 1248 2107 4720 7196 7370 65
Size: 1183 859 2613 2476 174 1396
Fragments arranged by size:
2613 2476 1396 1183 859 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3715 4395 7345 7532 1700
Size: 110 1905 680 2950 187 2869
Fragments arranged by size:
2950 2869 1905 680 187 110
BsrGI T'GTAC_A
Cuts at: 96 2121 96
Size: 2025 6676
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8701
BssSI C'ACGA_G
Cuts at: 4821 6761 7773 4821
Size: 1940 1012 5749
Fragments arranged by size:
5749 1940 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2479 2719 3127 3310 3493 3884 4085 794
Size: 1685 240 408 183 183 391 201 5410
Fragments arranged by size:
5410 1685 408 391 240 201 183 183
BstBI TT'CG_AA
Cuts at: 7505 7505
Size: 8701
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8701
BstXI CCAn_nnnn'nTGG
Cuts at: 2680 4823 2680
Size: 2143 6558
BstYI r'GATC_y
Cuts at: 1972 2073 2412 4870 4878 5034 5067 5829 6031
Size: 101 339 2458 8 156 33 762 202
Cuts at: 6031 6398 6783 6800 8252 8338 8350 8696 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2458 1977 1452 762 385 367 346 339
202 156 101 86 33 17 12 8
Bsu36I CC'TnA_GG
Cuts at: 2417 4214 2417
Size: 1797 6904
BtgI C'CryG_G
Cuts at: 513 1065 2004 2547 2841 2883 3001 4464 4599
Size: 552 939 543 294 42 118 1463 135
Cuts at: 4599 4659 5331 5415 513
Size: 60 672 84 3799
Fragments arranged by size:
3799 1463 939 672 552 543 294 135
118 84 60 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2233 2587 4587 5383 487
Size: 683 56 1007 354 2000 796 3805
Fragments arranged by size:
3805 2000 1007 796 683 354 56
BtsI GCAGTG_nn'
Cuts at: 3882 4032 4507 4903 5759 6217 7011 7031 3882
Size: 150 475 396 856 458 794 20 5552
Fragments arranged by size:
5552 856 794 475 458 396 150 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8666
DraI TTT'AAA
Cuts at: 3111 6005 3111
Size: 2894 5807
DraIII CAC_nnn'GTG
Cuts at: 2449 2449
Size: 8701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5705 7708 809
Size: 507 4389 2003 1802
Fragments arranged by size:
4389 2003 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3615 4067 4388
Size: 54 1425 50 275 18 1785 452 321
Cuts at: 4388 6148 7037 8666 8
Size: 1760 889 1629 43
Fragments arranged by size:
1785 1760 1629 1425 889 452 321 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6148 1537
Size: 275 4336 4090
Fragments arranged by size:
4336 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 2273 2735 3570 4677 6631 1353
Size: 837 83 462 835 1107 1954 3423
Fragments arranged by size:
3423 1954 1107 837 835 462 83
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2041 2742 3353 3905 3968 4096
Size: 1064 138 452 701 611 552 63 128
Cuts at: 4096 7288 7662 7808 387
Size: 3192 374 146 1280
Fragments arranged by size:
3192 1280 1064 701 611 552 452 374
146 138 128 63
EcoICRI GAG'CTC
Cuts at: 4851 5412 5882 4851
Size: 561 470 7670
Fragments arranged by size:
7670 561 470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 2577 2876 3062 3063 3314 3383
Size: 393 767 178 299 186 1 251 69
Cuts at: 3383 3392 3452 3791 3960 4143 4316 4701 4911
Size: 9 60 339 169 183 173 385 210
Cuts at: 4911 5222 5536 6764 7361 8148 8416 1239
Size: 311 314 1228 597 787 268 1524
Fragments arranged by size:
1524 1228 787 767 597 393 385 339
314 311 299 268 251 210 186 183
178 173 169 69 60 9 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2381 4280 2381
Size: 1899 6802
EcoO109I rG'GnC_Cy
Cuts at: 2062 3641 3674 3675 3683 3763 3944 3994 4017
Size: 1579 33 1 8 80 181 50 23
Cuts at: 4017 4062 4163 4164 4441 4632 4837 5434 2062
Size: 45 101 1 277 191 205 597 5329
Fragments arranged by size:
5329 1579 597 277 205 191 181 101
80 50 45 33 23 8 1 1
EcoRI G'AATT_C
Cuts at: 3922 6010 6388 3922
Size: 2088 378 6235
Fragments arranged by size:
6235 2088 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2881 2881
Size: 8701
FseI GG_CCGG'CC
Cuts at: 4399 4399
Size: 8701
FspI TGC'GCA
Cuts at: 7207 7207
Size: 8701
HaeII r_GCGC'y
Cuts at: 1678 3223 7848 1678
Size: 1545 4625 2531
Fragments arranged by size:
4625 2531 1545
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2256 2288 2639 2671 3894
Size: 32 135 32 684 32 351 32 1223
Cuts at: 3894 3897 3920 3926 3929 3952 4367 4399 4460
Size: 3 23 6 3 23 415 32 61
Cuts at: 4460 4492 4802 4834 5567 5599 7342 7374 7416
Size: 32 310 32 733 32 1743 32 42
Cuts at: 7416 7448 1373
Size: 32 2626
Fragments arranged by size:
2626 1743 1223 733 684 415 351 310
135 61 42 32 32 32 32 32
32 32 32 32 32 23 23 6
3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 4990 5095 5506 5581 5716
Size: 988 281 1113 1939 105 411 75 135
Cuts at: 5716 6045 6291 669
Size: 329 246 3079
Fragments arranged by size:
3079 1939 1113 988 411 329 281 246
135 105 75
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8701
HpaI GTT'AAC
Cuts at: 6291 6291
Size: 8701
KasI G'GCGC_C
Cuts at: 3219 3219
Size: 8701
KpnI G_GTAC'C
Cuts at: 6026 6026
Size: 8701
MfeI C'AATT_G
Cuts at: 6300 6300
Size: 8701
MluI A'CGCG_T
Cuts at: 8577 8577
Size: 8701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5817 7815 7999 1305
Size: 4512 1998 184 2007
Fragments arranged by size:
4512 2007 1998 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3617 10
Size: 54 1425 343 1785 5094
Fragments arranged by size:
5094 1785 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2436 2678 3035 4601 4661 5361 5612
Size: 648 1270 242 357 1566 60 700 251
Cuts at: 5612 5678 6659 7018 7177 518
Size: 66 981 359 159 2042
Fragments arranged by size:
2042 1566 1270 981 700 648 359 357
251 242 159 66 60
MspA1I CmG'CkG
Cuts at: 1606 2152 2536 2976 3127 3184 3190 3241 3259
Size: 546 384 440 151 57 6 51 18
Cuts at: 3259 3286 3370 3382 3409 3545 4957 5530 6117
Size: 27 84 12 27 136 1412 573 587
Cuts at: 6117 6794 7942 8187 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1412 1148 677 587 573 546 440
384 245 151 136 84 57 51 27
27 18 12 6
NaeI GCC'GGC
Cuts at: 4397 4397
Size: 8701
NarI GG'CG_CC
Cuts at: 3220 3220
Size: 8701
NcoI C'CATG_G
Cuts at: 513 1065 2004 3001 4464 4599 4659 513
Size: 552 939 997 1463 135 60 4555
Fragments arranged by size:
4555 1463 997 939 552 135 60
NdeI CA'TA_TG
Cuts at: 387 3778 387
Size: 3391 5310
NgoMIV G'CCGG_C
Cuts at: 4395 4395
Size: 8701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8701
NotI GC'GGCC_GC
Cuts at: 6148 6148
Size: 8701
NspI r_CATG'y
Cuts at: 1132 1229 2138 2221 2713 2811 3304 3425 4706
Size: 97 909 83 492 98 493 121 1281
Cuts at: 4706 6059 7604 1132
Size: 1353 1545 2229
Fragments arranged by size:
2229 1545 1353 1281 909 493 492 121
98 97 83
PasI CC'CwG_GG
Cuts at: 1348 3671 4352 4559 1348
Size: 2323 681 207 5490
Fragments arranged by size:
5490 2323 681 207
PciI A'CATG_T
Cuts at: 2807 7600 2807
Size: 4793 3908
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2775 5191 5572 5803 1108
Size: 1667 2416 381 231 4006
Fragments arranged by size:
4006 2416 1667 381 231
PfoI T'CCnGG_A
Cuts at: 2871 2930 2871
Size: 59 8642
PmeI GTTT'AAAC
Cuts at: 6005 6005
Size: 8701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4963 4995 6770 6802 8283 8315 4963
Size: 32 1775 32 1481 32 5349
Fragments arranged by size:
5349 1775 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2062 3683 3763 3944 4017 4062 4632 2062
Size: 1621 80 181 73 45 570 6131
Fragments arranged by size:
6131 1621 570 181 80 73 45
PshAI GACnn'nnGTC
Cuts at: 1613 2808 1613
Size: 1195 7506
PsiI TTA'TAA
Cuts at: 6271 6271
Size: 8701
PspOMI G'GGCC_C
Cuts at: 3561 3674 4163 5013 5433 3561
Size: 113 489 850 420 6829
Fragments arranged by size:
6829 850 489 420 113
PstI C_TGCA'G
Cuts at: 830 2408 3132 3270 3414 3444 3813 4962 5754
Size: 1578 724 138 144 30 369 1149 792
Cuts at: 5754 6053 830
Size: 299 3478
Fragments arranged by size:
3478 1578 1149 792 724 369 299 144
138 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8701
PvuII CAG'CTG
Cuts at: 2152 2536 3127 3370 3409 4957 2152
Size: 384 591 243 39 1548 5896
Fragments arranged by size:
5896 1548 591 384 243 39
SacI G_AGCT'C
Cuts at: 4853 5414 5884 4853
Size: 561 470 7670
Fragments arranged by size:
7670 561 470
SalI G'TCGA_C
Cuts at: 1936 3049 5504 5579 6043 1936
Size: 1113 2455 75 464 4594
Fragments arranged by size:
4594 2455 1113 464 75
SanDI GG'GwC_CC
Cuts at: 2062 3763 4062 4632 2062
Size: 1701 299 570 6131
Fragments arranged by size:
6131 1701 570 299
SapI GCTCTTCn'nnn_
Cuts at: 2273 2273
Size: 8701
SbfI CC_TGCA'GG
Cuts at: 3813 6053 3813
Size: 2240 6461
ScaI AGT'ACT
Cuts at: 2231 6949 8661 2231
Size: 4718 1712 2271
Fragments arranged by size:
4718 2271 1712
SexAI A'CCwGG_T
Cuts at: 2349 2349
Size: 8701
SfcI C'TryA_G
Cuts at: 826 1045 2404 3128 3266 3410 3440 3809 4958
Size: 219 1359 724 138 144 30 369 1149
Cuts at: 4958 5750 6049 7184 7865 8056 826
Size: 792 299 1135 681 191 1471
Fragments arranged by size:
1471 1359 1149 1135 792 724 681 369
299 219 191 144 138 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 4396 4396
Size: 8701
SfoI GGC'GCC
Cuts at: 3221 3221
Size: 8701
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8701
SmaI CCC'GGG
Cuts at: 1872 2289 5012 6028 1872
Size: 417 2723 1016 4545
Fragments arranged by size:
4545 2723 1016 417
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2762 2909 3431 5405 5534 5891
Size: 197 926 819 147 522 1974 129 357
Cuts at: 5891 6805 7706 7968 820
Size: 914 901 262 1553
Fragments arranged by size:
1974 1553 926 914 901 819 522 357
262 197 147 129
SnaBI TAC'GTA
Cuts at: 493 6492 493
Size: 5999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8701
SphI G_CATG'C
Cuts at: 1132 3425 4706 6059 1132
Size: 2293 1281 1353 3774
Fragments arranged by size:
3774 2293 1353 1281
SspI AAT'ATT
Cuts at: 5 52 6625 5
Size: 47 6573 2081
Fragments arranged by size:
6573 2081 47
StuI AGG'CCT
Cuts at: 1517 3665 1517
Size: 2148 6553
StyI C'CwwG_G
Cuts at: 513 1065 2004 3001 3612 4002 4464 4599 4659
Size: 552 939 997 611 390 462 135 60
Cuts at: 4659 6140 513
Size: 1481 3074
Fragments arranged by size:
3074 1481 997 939 611 552 462 390
135 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2854 2940 3895 4015 4774 4919 5151
Size: 1255 1274 86 955 120 759 145 232
Cuts at: 5151 6728 6745 6898 7083 7528 325
Size: 1577 17 153 185 445 1498
Fragments arranged by size:
1577 1498 1274 1255 955 759 445 232
185 153 145 120 86 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2121 2229 2970
Size: 274 80 33 51 1443 144 108 741
Cuts at: 2970 5043 6947 8659 96
Size: 2073 1904 1712 138
Fragments arranged by size:
2073 1904 1712 1443 741 274 144 138
108 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3747 3779 8661 8693 3747
Size: 32 4882 32 3755
Fragments arranged by size:
4882 3755 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4250 4589 4739 5861 1316
Size: 2934 339 150 1122 4156
Fragments arranged by size:
4156 2934 1122 339 150
XbaI T'CTAG_A
Cuts at: 6037 6037
Size: 8701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 3829 4462 4489 5800 2167
Size: 1662 633 27 1311 5068
Fragments arranged by size:
5068 1662 1311 633 27
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8701
XmaI C'CCGG_G
Cuts at: 1870 2287 5010 6026 1870
Size: 417 2723 1016 4545
Fragments arranged by size:
4545 2723 1016 417
XmnI GAAnn'nnTTC
Cuts at: 2564 5054 6830 2564
Size: 2490 1776 4435
Fragments arranged by size:
4435 2490 1776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4615 6509 276
Size: 53 83 186 936 3081 1894 2468
Fragments arranged by size:
3081 2468 1894 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BstZ17I EcoRV
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SrfI SwaI