Restriction map for
pFN21AE3595
(Circular) (Six-base) MAPSORT of: pFN21AE3595.seq Check: 9891 from: 1 to: 5767
pFN21AE3595 5767 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2669 2669
Size: 5767
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3110 1937
Size: 1173 4594
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3830 5214 5482 1632
Size: 2198 1384 268 1917
Fragments arranged by size:
2198 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 4666 5643 1203
Size: 3463 977 1327
Fragments arranged by size:
3463 1327 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2097 1184
Size: 913 4854
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2347 2379 2347
Size: 32 5735
AlwNI CAG_nnn'CTG
Cuts at: 2498 3510 5082 2498
Size: 1012 1572 3183
Fragments arranged by size:
3183 1572 1012
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 2587 2774 3002 3076 3302 3454 5563 1493
Size: 1094 187 228 74 226 152 2109 1697
Fragments arranged by size:
2109 1697 1094 228 226 187 152 74
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 3092 1870
Size: 73 1149 4545
Fragments arranged by size:
4545 1149 73
BamHI G'GATC_C
Cuts at: 2637 3097 3464 2637
Size: 460 367 4940
Fragments arranged by size:
4940 460 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3088 4543 618
Size: 325 217 627 1301 1455 1842
Fragments arranged by size:
1842 1455 1301 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2373 3086 1391
Size: 982 713 4072
Fragments arranged by size:
4072 982 713
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4796
BbvCI CC'TCA_GC
Cuts at: 2025 2025
Size: 5767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2414 2448 3958 3992 1903
Size: 34 477 34 1510 34 3678
Fragments arranged by size:
3678 1510 477 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BfrBI ATG'CAT
Cuts at: 2734 2734
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4378 136
Size: 107 122 71 631 605 2706 1525
Fragments arranged by size:
2706 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5762 5762
Size: 5767
BlpI GC'TnA_GC
Cuts at: 3184 3184
Size: 5767
Bme1580I G_kGCm'C
Cuts at: 1790 3828 4984 1790
Size: 2038 1156 2573
Fragments arranged by size:
2573 2038 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2285 3498 4456
Size: 416 53 83 399 24 868 1213 958
Cuts at: 4456 442
Size: 1753
Fragments arranged by size:
1753 1213 958 868 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 4427 1239
Size: 986 2202 2579
Fragments arranged by size:
2579 2202 986
Bpu10I CC'TnA_GC
Cuts at: 2025 2367 2025
Size: 342 5425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3032 3892 4757 5055 3032
Size: 860 865 298 3744
Fragments arranged by size:
3744 865 860 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4430 882
Size: 617 2931 2219
Fragments arranged by size:
2931 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 2165 5761 2165
Size: 3596 2171
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3574 3956 4591 275
Size: 53 83 186 936 2041 382 635 1451
Fragments arranged by size:
2041 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4200 4598 4872 5019 5430 4200
Size: 398 274 147 411 4537
Fragments arranged by size:
4537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2347 2377 1178
Size: 30 165 30 944 30 4568
Fragments arranged by size:
4568 944 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 1186
Size: 1177 4590
BseYI C'CCAG_C
Cuts at: 2119 2126 4970 5466 2119
Size: 7 2844 496 2420
Fragments arranged by size:
2844 2420 496 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2697 1163
Size: 573 961 4233
Fragments arranged by size:
4233 961 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3217 3978 5006 1540
Size: 275 186 1216 761 1028 2301
Fragments arranged by size:
2301 1216 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2147 2373 2529 3828 3913 4984 2147
Size: 226 156 1299 85 1071 2930
Fragments arranged by size:
2930 1299 1071 226 156 85
BsmI GAATG_Cn'
Cuts at: 2204 3278 3371 2204
Size: 1074 93 4600
Fragments arranged by size:
4600 1074 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2147 2373 2529 3086 3828 3913 4984
Size: 399 357 226 156 557 742 85 1071
Cuts at: 4984 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 557 399 357 226 156
85
BspEI T'CCGG_A
Cuts at: 5430 5430
Size: 5767
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2669 3122 844
Size: 1825 453 3489
Fragments arranged by size:
3489 1825 453
BsrBI CCG'CTC
Cuts at: 1394 1823 3654 1394
Size: 429 1831 3507
Fragments arranged by size:
3507 1831 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4262 4436 65
Size: 1183 3014 174 1396
Fragments arranged by size:
3014 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4411 4598 1700
Size: 110 2601 187 2869
Fragments arranged by size:
2869 2601 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5767
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 2372 3827 4839 2372
Size: 1455 1012 3300
Fragments arranged by size:
3300 1455 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2524 794
Size: 1730 4037
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2618 2637 3097 3464 3849 3866 5318 5404
Size: 646 19 460 367 385 17 1452 86
Cuts at: 5404 5416 5762 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 646 460 385 367 346 86
19 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4276
Fragments arranged by size:
4276 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5028
Fragments arranged by size:
5028 683 56
BtsI GCAGTG_nn'
Cuts at: 2641 3283 4077 4097 2641
Size: 642 794 20 4311
Fragments arranged by size:
4311 794 642 20
ClaI AT'CG_AT
Cuts at: 1554 2283 1554
Size: 729 5038
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 3214 4103
Size: 54 1425 50 275 18 210 1174 889
Cuts at: 4103 5732 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1174 889 275 210 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 1537
Size: 275 1402 4090
Fragments arranged by size:
4090 1402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3068 3697 1353
Size: 1715 629 3423
Fragments arranged by size:
3423 1715 629
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4354 4728 4874 387
Size: 1064 138 2765 374 146 1280
Fragments arranged by size:
2765 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2371 2371
Size: 5767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 3830 4427 5214 5482 1239
Size: 393 593 1605 597 787 268 1524
Fragments arranged by size:
1605 1524 787 597 593 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2825 2825
Size: 5767
EcoO109I rG'GnC_Cy
Cuts at: 2818 2818
Size: 5767
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 5767
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 4914 1678
Size: 3236 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2476 2523 2555 4408
Size: 32 135 32 872 32 47 32 1853
Cuts at: 4408 4440 4482 4514 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1853 872 135 47 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4881 5065 1305
Size: 3576 184 2007
Fragments arranged by size:
3576 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2042 10
Size: 54 1425 343 210 3735
Fragments arranged by size:
3735 1425 343 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2178 3725 4084 4243 518
Size: 648 1012 1547 359 159 2042
Fragments arranged by size:
2042 1547 1012 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3183 3860 5008 5253 1606
Size: 1577 677 1148 245 2120
Fragments arranged by size:
2120 1577 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4276
Fragments arranged by size:
4276 939 552
NdeI CA'TA_TG
Cuts at: 387 2762 387
Size: 2375 3392
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NsiI A_TGCA'T
Cuts at: 2736 2736
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 3125 4670 1132
Size: 97 1896 1545 2229
Fragments arranged by size:
2229 1896 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5767
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2347 2379 3836 3868 5349 5381 2347
Size: 32 1457 32 1481 32 2733
Fragments arranged by size:
2733 1481 1457 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2818 2818
Size: 5767
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PstI C_TGCA'G
Cuts at: 830 2076 2241 2437 3119 830
Size: 1246 165 196 682 3478
Fragments arranged by size:
3478 1246 682 196 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
SacI G_AGCT'C
Cuts at: 2373 2373
Size: 5767
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 2072 2237 2433 2962 2982 3115 4250
Size: 219 1027 165 196 529 20 133 1135
Cuts at: 4250 4931 5122 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1027 681 529 219 196 191
165 133 20
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 3094 1872
Size: 1222 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3047 3871 4772 5034 820
Size: 197 926 1104 824 901 262 1553
Fragments arranged by size:
1553 1104 926 901 824 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 3206 513
Size: 552 939 48 1154 3074
Fragments arranged by size:
3074 1154 939 552 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2111 3794 3811 3964 4149 4594 325
Size: 1255 531 1683 17 153 185 445 1498
Fragments arranged by size:
1683 1498 1255 531 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4013 5725 96
Size: 274 80 33 51 1443 2036 1712 138
Fragments arranged by size:
2036 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2389 2421 5727 5759 2389
Size: 32 3306 32 2397
Fragments arranged by size:
3306 2397 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2272 2272
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 3092 1870
Size: 1222 4545
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI