Restriction map for
pFN21AE3592
(Circular) (Six-base) MAPSORT of: pFN21AE3592.seq Check: 547 from: 1 to: 5449
pFN21AE3592 5449 bp
With 182 enzymes: SgfI *
August 8, 2011 18:07 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3259 278
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2792 1937
Size: 855 4594
Acc65I G'GTAC_C
Cuts at: 2770 2770
Size: 5449
AclI AA'CG_TT
Cuts at: 1563 3576 3949 1563
Size: 2013 373 3063
Fragments arranged by size:
3063 2013 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2362 2596 3512 4896 5164 1632
Size: 730 234 916 1384 268 1917
Fragments arranged by size:
1917 1384 916 730 268 234
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5252
AflIII A'CryG_T
Cuts at: 1203 4348 5325 1203
Size: 3145 977 1327
Fragments arranged by size:
3145 1327 977
AgeI A'CCGG_T
Cuts at: 2217 4280 2217
Size: 2063 3386
AhdI GACnn_n'nnGTC
Cuts at: 4178 4178
Size: 5449
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5449
AlwNI CAG_nnn'CTG
Cuts at: 2714 3192 4764 2714
Size: 478 1572 3399
Fragments arranged by size:
3399 1572 478
ApaLI G'TGCA_C
Cuts at: 2333 3506 4662 2333
Size: 1173 1156 3120
Fragments arranged by size:
3120 1173 1156
ApoI r'AATT_y
Cuts at: 1493 2758 2984 3136 5245 1493
Size: 1265 226 152 2109 1697
Fragments arranged by size:
2109 1697 1265 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4003 160
Size: 872 2971 1606
Fragments arranged by size:
2971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2075 2774 1870
Size: 73 120 12 699 4545
Fragments arranged by size:
4545 699 120 73 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2303 2336 2303
Size: 33 5416
BamHI G'GATC_C
Cuts at: 2779 3146 2779
Size: 367 5082
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2770 4225 618
Size: 325 217 627 221 762 1455 1842
Fragments arranged by size:
1842 1455 762 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2768 1391
Size: 1377 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2088 2588 928
Size: 971 189 500 3789
Fragments arranged by size:
3789 971 500 189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2450 2484 3640 3674 1903
Size: 34 513 34 1156 34 3678
Fragments arranged by size:
3678 1156 513 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3341 4551 3341
Size: 1210 4239
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5449
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2728 4060 136
Size: 107 122 71 631 605 1056 1332 1525
Fragments arranged by size:
1525 1332 1056 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5444 5444
Size: 5449
BlpI GC'TnA_GC
Cuts at: 2866 2866
Size: 5449
Bme1580I G_kGCm'C
Cuts at: 1790 2195 2265 2337 3510 4666 1790
Size: 405 70 72 1173 1156 2573
Fragments arranged by size:
2573 1173 1156 405 72 70
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5449
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3180 4138 442
Size: 416 53 83 399 24 1763 958 1753
Fragments arranged by size:
1763 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2589 2637 4109 1239
Size: 1350 48 1472 2579
Fragments arranged by size:
2579 1472 1350 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2365 3574 4439 4737 2365
Size: 1209 865 298 3077
Fragments arranged by size:
3077 1209 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4112 882
Size: 617 2613 2219
Fragments arranged by size:
2613 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3240 493
Size: 711 2036 2702
Fragments arranged by size:
2702 2036 711
BsaBI GATnn'nnATC
Cuts at: 5443 5443
Size: 5449
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3256 3638 4273 275
Size: 53 83 186 936 1723 382 635 1451
Fragments arranged by size:
1723 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2217 3882 4280 4554 4701 5112 2217
Size: 1665 398 274 147 411 2554
Fragments arranged by size:
2554 1665 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2191 2221 2481 2511 1178
Size: 30 165 30 788 30 260 30 4116
Fragments arranged by size:
4116 788 260 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2212 2640 1186
Size: 1026 428 3995
Fragments arranged by size:
3995 1026 428
BseYI C'CCAG_C
Cuts at: 2673 4652 5148 2673
Size: 1979 496 2974
Fragments arranged by size:
2974 1979 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 2192 1163
Size: 573 414 42 4420
Fragments arranged by size:
4420 573 414 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2899 3660 4688 1540
Size: 275 186 898 761 1028 2301
Fragments arranged by size:
2301 1028 898 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 3510 3595 4666 2337
Size: 1173 85 1071 3120
Fragments arranged by size:
3120 1173 1071 85
BsmI GAATG_Cn'
Cuts at: 2960 3053 2960
Size: 93 5356
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 2265 2337 2768 3510 3595 4666
Size: 399 405 70 72 431 742 85 1071
Cuts at: 4666 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 431 405 399 85 72
70
BspEI T'CCGG_A
Cuts at: 5112 5112
Size: 5449
BspHI T'CATG_A
Cuts at: 3338 5068 3338
Size: 1730 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2804 844
Size: 1960 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3336 1394
Size: 429 1513 3507
Fragments arranged by size:
3507 1513 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2313 3944 4118 65
Size: 1183 1065 1631 174 1396
Fragments arranged by size:
1631 1396 1183 1065 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2217 4093 4280 1700
Size: 110 407 1876 187 2869
Fragments arranged by size:
2869 1876 407 187 110
BsrGI T'GTAC_A
Cuts at: 96 2397 96
Size: 2301 3148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5449
BssSI C'ACGA_G
Cuts at: 2194 3509 4521 2194
Size: 1315 1012 3122
Fragments arranged by size:
3122 1315 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5449
BstBI TT'CG_AA
Cuts at: 4253 4253
Size: 5449
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5449
BstYI r'GATC_y
Cuts at: 1972 2779 3146 3531 3548 5000 5086 5098 5444
Size: 807 367 385 17 1452 86 12 346
Cuts at: 5444 1972
Size: 1977
Fragments arranged by size:
1977 1452 807 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2013 2013
Size: 5449
BtgI C'CryG_G
Cuts at: 513 1065 2004 2361 2493 513
Size: 552 939 357 132 3469
Fragments arranged by size:
3469 939 552 357 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4710
Fragments arranged by size:
4710 683 56
BtsI GCAGTG_nn'
Cuts at: 2965 3759 3779 2965
Size: 794 20 4635
Fragments arranged by size:
4635 794 20
ClaI AT'CG_AT
Cuts at: 1554 2586 1554
Size: 1032 4417
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5414
DraI TTT'AAA
Cuts at: 2753 2753
Size: 5449
DraIII CAC_nnn'GTG
Cuts at: 2188 2188
Size: 5449
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4456 809
Size: 507 3140 1802
Fragments arranged by size:
3140 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2896 3785 5414
Size: 54 1425 50 275 18 1066 889 1629
Cuts at: 5414 8
Size: 43
Fragments arranged by size:
1629 1425 1066 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2896 1537
Size: 275 1084 4090
Fragments arranged by size:
4090 1084 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2459 3379 1353
Size: 1106 920 3423
Fragments arranged by size:
3423 1106 920
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4036 4410 4556 387
Size: 1064 138 2447 374 146 1280
Fragments arranged by size:
2447 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2362 2589 2596 2637 3512 4109 4896
Size: 393 730 227 7 41 875 597 787
Cuts at: 4896 5164 1239
Size: 268 1524
Fragments arranged by size:
1524 875 787 730 597 393 268 227
41 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2652 2652
Size: 5449
EcoO109I rG'GnC_Cy
Cuts at: 2669 2669
Size: 5449
EcoRI G'AATT_C
Cuts at: 2758 3136 2758
Size: 378 5071
EcoRV GAT'ATC
Cuts at: 2370 2370
Size: 5449
FspI TGC'GCA
Cuts at: 3955 3955
Size: 5449
HaeII r_GCGC'y
Cuts at: 1678 4596 1678
Size: 2918 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2191 2223 2481 2513 4090
Size: 32 135 32 619 32 258 32 1577
Cuts at: 4090 4122 4164 4196 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1577 619 258 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2793 3039 669
Size: 988 281 855 246 3079
Fragments arranged by size:
3079 988 855 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5449
HpaI GTT'AAC
Cuts at: 3039 3039
Size: 5449
KpnI G_GTAC'C
Cuts at: 2774 2774
Size: 5449
MfeI C'AATT_G
Cuts at: 3048 3048
Size: 5449
MluI A'CGCG_T
Cuts at: 5325 5325
Size: 5449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4563 4747 1305
Size: 3258 184 2007
Fragments arranged by size:
3258 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3627
Fragments arranged by size:
3627 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 3407 3766 3925 518
Size: 648 1194 1047 359 159 2042
Fragments arranged by size:
2042 1194 1047 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2865 3542 4690 4935 1606
Size: 1259 677 1148 245 2120
Fragments arranged by size:
2120 1259 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2361 513
Size: 552 939 357 3601
Fragments arranged by size:
3601 939 552 357
NdeI CA'TA_TG
Cuts at: 387 2637 387
Size: 2250 3199
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5449
NotI GC'GGCC_GC
Cuts at: 2896 2896
Size: 5449
NspI r_CATG'y
Cuts at: 1132 1229 2807 4352 1132
Size: 97 1578 1545 2229
Fragments arranged by size:
2229 1578 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5449
PciI A'CATG_T
Cuts at: 4348 4348
Size: 5449
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2714 1108
Size: 1606 3843
PfoI T'CCnGG_A
Cuts at: 2653 2653
Size: 5449
PmeI GTTT'AAAC
Cuts at: 2753 2753
Size: 5449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 5031 5063 3518
Size: 32 1481 32 3904
Fragments arranged by size:
3904 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2669 2669
Size: 5449
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5449
PsiI TTA'TAA
Cuts at: 3019 3019
Size: 5449
PstI C_TGCA'G
Cuts at: 830 2801 830
Size: 1971 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5449
SalI G'TCGA_C
Cuts at: 1936 2791 1936
Size: 855 4594
SbfI CC_TGCA'GG
Cuts at: 2801 2801
Size: 5449
ScaI AGT'ACT
Cuts at: 3697 5409 3697
Size: 1712 3737
SfcI C'TryA_G
Cuts at: 826 1045 2797 3932 4613 4804 826
Size: 219 1752 1135 681 191 1471
Fragments arranged by size:
1752 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5449
SmaI CCC'GGG
Cuts at: 1872 2065 2776 1872
Size: 193 711 4545
Fragments arranged by size:
4545 711 193
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2344 3553 4454 4716 820
Size: 197 926 401 1209 901 262 1553
Fragments arranged by size:
1553 1209 926 901 401 262 197
SnaBI TAC'GTA
Cuts at: 493 3240 493
Size: 2747 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5449
SphI G_CATG'C
Cuts at: 1132 2807 1132
Size: 1675 3774
SspI AAT'ATT
Cuts at: 5 52 3373 5
Size: 47 3321 2081
Fragments arranged by size:
3321 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5449
StyI C'CwwG_G
Cuts at: 513 1065 2004 2361 2888 513
Size: 552 939 357 527 3074
Fragments arranged by size:
3074 939 552 527 357
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3476 3493 3646 3831 4276 325
Size: 1255 1896 17 153 185 445 1498
Fragments arranged by size:
1896 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2397 3695 5407
Size: 274 80 33 51 1443 420 1298 1712
Cuts at: 5407 96
Size: 138
Fragments arranged by size:
1712 1443 1298 420 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5409 5441 5409
Size: 32 5417
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5449
XbaI T'CTAG_A
Cuts at: 2785 2785
Size: 5449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 2185
Size: 5449
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5449
XmaI C'CCGG_G
Cuts at: 1870 2063 2774 1870
Size: 193 711 4545
Fragments arranged by size:
4545 711 193
XmnI GAAnn'nnTTC
Cuts at: 3578 3578
Size: 5449
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3257 276
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI