Restriction map for
pFN21AE3586
(Circular) (Six-base) MAPSORT of: pFN21AE3586.seq Check: 2315 from: 1 to: 5320
pFN21AE3586 5320 bp
With 182 enzymes: SgfI *
March 28, 2012 13:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3130 278
Size: 53 83 186 936 1594 2468
Fragments arranged by size:
2468 1594 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2663 1937
Size: 726 4594
Acc65I G'GTAC_C
Cuts at: 2641 2641
Size: 5320
AclI AA'CG_TT
Cuts at: 1563 3447 3820 1563
Size: 1884 373 3063
Fragments arranged by size:
3063 1884 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3383 4767 5035 1632
Size: 1751 1384 268 1917
Fragments arranged by size:
1917 1751 1384 268
AfeI AGC'GCT
Cuts at: 2333 2333
Size: 5320
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5123
AflIII A'CryG_T
Cuts at: 1203 2122 4219 5196 1203
Size: 919 2097 977 1327
Fragments arranged by size:
2097 1327 977 919
AgeI A'CCGG_T
Cuts at: 4151 4151
Size: 5320
AhdI GACnn_n'nnGTC
Cuts at: 4049 4049
Size: 5320
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5320
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5320
AlwNI CAG_nnn'CTG
Cuts at: 2189 3063 4635 2189
Size: 874 1572 2874
Fragments arranged by size:
2874 1572 874
ApaI G_GGCC'C
Cuts at: 2023 2023
Size: 5320
ApaLI G'TGCA_C
Cuts at: 3377 4533 3377
Size: 1156 4164
ApoI r'AATT_y
Cuts at: 1493 2629 2855 3007 5116 1493
Size: 1136 226 152 2109 1697
Fragments arranged by size:
2109 1697 1136 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3874 160
Size: 872 2842 1606
Fragments arranged by size:
2842 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5320
AvaI C'yCGr_G
Cuts at: 1870 1943 2645 1870
Size: 73 702 4545
Fragments arranged by size:
4545 702 73
BamHI G'GATC_C
Cuts at: 2650 3017 2650
Size: 367 4953
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2641 4096 618
Size: 325 217 627 854 1455 1842
Fragments arranged by size:
1842 1455 854 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2023 2639 1391
Size: 632 616 4072
Fragments arranged by size:
4072 632 616
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2065 2421 928
Size: 971 166 356 3827
Fragments arranged by size:
3827 971 356 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3511 3545 1903
Size: 34 1574 34 3678
Fragments arranged by size:
3678 1574 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3212 4422 3212
Size: 1210 4110
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5320
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3931 136
Size: 107 122 71 631 605 2259 1525
Fragments arranged by size:
2259 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5315 5315
Size: 5320
BlpI GC'TnA_GC
Cuts at: 2737 2737
Size: 5320
Bme1580I G_kGCm'C
Cuts at: 1790 2023 3381 4537 1790
Size: 233 1358 1156 2573
Fragments arranged by size:
2573 1358 1156 233
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5320
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2245 2283 3051
Size: 416 53 83 399 24 828 38 768
Cuts at: 3051 4009 442
Size: 958 1753
Fragments arranged by size:
1753 958 828 768 416 399 83 53
38 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3980 1239
Size: 2741 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2446 3445 4310 4608 2446
Size: 999 865 298 3158
Fragments arranged by size:
3158 999 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3983 882
Size: 617 2484 2219
Fragments arranged by size:
2484 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2143 3111 493
Size: 711 939 968 2702
Fragments arranged by size:
2702 968 939 711
BsaBI GATnn'nnATC
Cuts at: 5314 5314
Size: 5320
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3127 3509 4144 275
Size: 53 83 186 936 1594 382 635 1451
Fragments arranged by size:
1594 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2100 3753 4151 4425 4572 4983 2100
Size: 1653 398 274 147 411 2437
Fragments arranged by size:
2437 1653 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2300 2330 2375 2405 1178
Size: 30 165 30 897 30 45 30 4093
Fragments arranged by size:
4093 897 165 45 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 1186
Size: 1057 4263
BseYI C'CCAG_C
Cuts at: 4523 5019 4523
Size: 496 4824
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 1163
Size: 573 411 4336
Fragments arranged by size:
4336 573 411
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2770 3531 4559 1540
Size: 275 186 769 761 1028 2301
Fragments arranged by size:
2301 1028 769 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3381 3466 4537 3381
Size: 85 1071 4164
Fragments arranged by size:
4164 1071 85
BsmI GAATG_Cn'
Cuts at: 2831 2924 2831
Size: 93 5227
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2639 3381 3466 4537 1391
Size: 399 233 616 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 616 399 233 85
BspEI T'CCGG_A
Cuts at: 2100 4983 2100
Size: 2883 2437
BspHI T'CATG_A
Cuts at: 3209 4939 3209
Size: 1730 3590
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2675 844
Size: 1831 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3207 1394
Size: 429 1384 3507
Fragments arranged by size:
3507 1384 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3815 3989 65
Size: 1183 2567 174 1396
Fragments arranged by size:
2567 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2283 3964 4151 1700
Size: 110 473 1681 187 2869
Fragments arranged by size:
2869 1681 473 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5320
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5320
BssSI C'ACGA_G
Cuts at: 3380 4392 3380
Size: 1012 4308
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2189 794
Size: 1395 3925
BstBI TT'CG_AA
Cuts at: 4124 4124
Size: 5320
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5320
BstXI CCAn_nnnn'nTGG
Cuts at: 2577 2577
Size: 5320
BstYI r'GATC_y
Cuts at: 1972 2650 3017 3402 3419 4871 4957 4969 5315
Size: 678 367 385 17 1452 86 12 346
Cuts at: 5315 1972
Size: 1977
Fragments arranged by size:
1977 1452 678 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2469 2469
Size: 5320
BtgI C'CryG_G
Cuts at: 513 1065 2004 2193 513
Size: 552 939 189 3640
Fragments arranged by size:
3640 939 552 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2524 487
Size: 683 56 1298 3283
Fragments arranged by size:
3283 1298 683 56
BtsI GCAGTG_nn'
Cuts at: 2282 2836 3630 3650 2282
Size: 554 794 20 3952
Fragments arranged by size:
3952 794 554 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5285
DraI TTT'AAA
Cuts at: 2624 2624
Size: 5320
DraIII CAC_nnn'GTG
Cuts at: 2179 2179
Size: 5320
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4327 809
Size: 507 3011 1802
Fragments arranged by size:
3011 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2767 3656
Size: 54 1425 50 275 18 219 718 889
Cuts at: 3656 5285 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 718 275 219 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2767 1537
Size: 275 955 4090
Fragments arranged by size:
4090 955 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2489 2570 3250 1353
Size: 1136 81 680 3423
Fragments arranged by size:
3423 1136 680 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3907 4281 4427 387
Size: 1064 138 2318 374 146 1280
Fragments arranged by size:
2318 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3383 3980 4767 5035 1239
Size: 393 1751 597 787 268 1524
Fragments arranged by size:
1751 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2354 2354
Size: 5320
EcoO109I rG'GnC_Cy
Cuts at: 2020 2020
Size: 5320
EcoRI G'AATT_C
Cuts at: 2629 3007 2629
Size: 378 4942
FspI TGC'GCA
Cuts at: 3826 3826
Size: 5320
HaeII r_GCGC'y
Cuts at: 1678 2335 4467 1678
Size: 657 2132 2531
Fragments arranged by size:
2531 2132 657
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 3961 3993 4035
Size: 32 135 32 728 32 1629 32 42
Cuts at: 4035 4067 1373
Size: 32 2626
Fragments arranged by size:
2626 1629 728 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2664 2910 669
Size: 988 281 726 246 3079
Fragments arranged by size:
3079 988 726 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5320
HpaI GTT'AAC
Cuts at: 2910 2910
Size: 5320
KpnI G_GTAC'C
Cuts at: 2645 2645
Size: 5320
MfeI C'AATT_G
Cuts at: 2919 2919
Size: 5320
MluI A'CGCG_T
Cuts at: 5196 5196
Size: 5320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4434 4618 1305
Size: 3129 184 2007
Fragments arranged by size:
3129 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3498
Fragments arranged by size:
3498 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 2409 3278 3637 3796 518
Size: 648 957 286 869 359 159 2042
Fragments arranged by size:
2042 957 869 648 359 286 159
MspA1I CmG'CkG
Cuts at: 1606 2054 2131 2239 2271 2421 2736 3413 4561
Size: 448 77 108 32 150 315 677 1148
Cuts at: 4561 4806 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 448 315 245 150 108
77 32
NcoI C'CATG_G
Cuts at: 513 1065 2004 2193 513
Size: 552 939 189 3640
Fragments arranged by size:
3640 939 552 189
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5320
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5320
NotI GC'GGCC_GC
Cuts at: 2767 2767
Size: 5320
NspI r_CATG'y
Cuts at: 1132 1229 2126 2678 4223 1132
Size: 97 897 552 1545 2229
Fragments arranged by size:
2229 1545 897 552 97
PasI CC'CwG_GG
Cuts at: 1348 2350 1348
Size: 1002 4318
PciI A'CATG_T
Cuts at: 2122 4219 2122
Size: 2097 3223
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5320
PmeI GTTT'AAAC
Cuts at: 2624 2624
Size: 5320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3389 3421 4902 4934 3389
Size: 32 1481 32 3775
Fragments arranged by size:
3775 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5320
PsiI TTA'TAA
Cuts at: 2890 2890
Size: 5320
PspOMI G'GGCC_C
Cuts at: 2019 2019
Size: 5320
PstI C_TGCA'G
Cuts at: 830 2045 2672 830
Size: 1215 627 3478
Fragments arranged by size:
3478 1215 627
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5320
PvuII CAG'CTG
Cuts at: 2131 2239 2131
Size: 108 5212
SalI G'TCGA_C
Cuts at: 1936 2662 1936
Size: 726 4594
SbfI CC_TGCA'GG
Cuts at: 2672 2672
Size: 5320
ScaI AGT'ACT
Cuts at: 3568 5280 3568
Size: 1712 3608
SexAI A'CCwGG_T
Cuts at: 2214 2214
Size: 5320
SfcI C'TryA_G
Cuts at: 826 1045 2041 2668 3803 4484 4675 826
Size: 219 996 627 1135 681 191 1471
Fragments arranged by size:
1471 1135 996 681 627 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5320
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5320
SmaI CCC'GGG
Cuts at: 1872 2647 1872
Size: 775 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2425 3424 4325 4587 820
Size: 197 926 482 999 901 262 1553
Fragments arranged by size:
1553 999 926 901 482 262 197
SnaBI TAC'GTA
Cuts at: 493 3111 493
Size: 2618 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5320
SphI G_CATG'C
Cuts at: 1132 2678 1132
Size: 1546 3774
SspI AAT'ATT
Cuts at: 5 52 3244 5
Size: 47 3192 2081
Fragments arranged by size:
3192 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5320
StyI C'CwwG_G
Cuts at: 513 1065 2004 2193 2256 2759 513
Size: 552 939 189 63 503 3074
Fragments arranged by size:
3074 939 552 503 189 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3347 3364 3517 3702 4147 325
Size: 1255 1767 17 153 185 445 1498
Fragments arranged by size:
1767 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 3566 5278
Size: 274 80 33 51 1443 225 1364 1712
Cuts at: 5278 96
Size: 138
Fragments arranged by size:
1712 1443 1364 274 225 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2449 2481 2550 2582 5280 5312 2449
Size: 32 69 32 2698 32 2457
Fragments arranged by size:
2698 2457 69 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2264 1316
Size: 948 4372
XbaI T'CTAG_A
Cuts at: 2656 2656
Size: 5320
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5320
XmaI C'CCGG_G
Cuts at: 1870 2645 1870
Size: 775 4545
XmnI GAAnn'nnTTC
Cuts at: 3449 3449
Size: 5320
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3128 276
Size: 53 83 186 936 1594 2468
Fragments arranged by size:
2468 1594 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI