Restriction map for
pFN21AE3584
(Circular) (Six-base) MAPSORT of: pFN21AE3584.seq Check: 9766 from: 1 to: 6004
pFN21AE3584 6004 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2604 2604
Size: 6004
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3347 1937
Size: 1410 4594
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2094 2256 2423 4067 5451 5719 1632
Size: 462 162 167 1644 1384 268 1917
Fragments arranged by size:
1917 1644 1384 462 268 167 162
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 2148 2424 2520 3285 4903 5880 1203
Size: 945 276 96 765 1618 977 1327
Fragments arranged by size:
1618 1327 977 945 765 276 96
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2167 3039 1184
Size: 983 872 4149
Fragments arranged by size:
4149 983 872
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2411 2443 2411
Size: 32 5972
AlwNI CAG_nnn'CTG
Cuts at: 3103 3747 5319 3103
Size: 644 1572 3788
Fragments arranged by size:
3788 1572 644
ApaLI G'TGCA_C
Cuts at: 4061 5217 4061
Size: 1156 4848
ApoI r'AATT_y
Cuts at: 1493 2338 3313 3539 3691 5800 1493
Size: 845 975 226 152 2109 1697
Fragments arranged by size:
2109 1697 975 845 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 3329 1870
Size: 73 1386 4545
Fragments arranged by size:
4545 1386 73
BamHI G'GATC_C
Cuts at: 2387 3334 3701 2387
Size: 947 367 4690
Fragments arranged by size:
4690 947 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2136 3030 3325 4780
Size: 325 217 627 264 85 894 295 1455
Cuts at: 4780 618
Size: 1842
Fragments arranged by size:
1842 1455 894 627 325 295 264 217
85
BanII G_rGCy'C
Cuts at: 1391 3118 3323 1391
Size: 1727 205 4072
Fragments arranged by size:
4072 1727 205
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2244 2727 928
Size: 971 345 483 4205
Fragments arranged by size:
4205 971 483 345
BbvCI CC'TCA_GC
Cuts at: 3087 3087
Size: 6004
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2207 2241 4195 4229 1903
Size: 34 270 34 1954 34 3678
Fragments arranged by size:
3678 1954 270 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3261 3896 5106 3261
Size: 635 1210 4159
Fragments arranged by size:
4159 1210 635
BclI T'GATC_A
Cuts at: 1548 2262 1548
Size: 714 5290
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4615 136
Size: 107 122 71 631 605 2943 1525
Fragments arranged by size:
2943 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2482 2737 5999 2482
Size: 255 3262 2487
Fragments arranged by size:
3262 2487 255
BlpI GC'TnA_GC
Cuts at: 3421 3421
Size: 6004
Bme1580I G_kGCm'C
Cuts at: 1790 3132 4065 5221 1790
Size: 1342 933 1156 2573
Fragments arranged by size:
2573 1342 1156 933
BmgBI CAC'GTC
Cuts at: 1318 2731 2803 1318
Size: 1413 72 4519
Fragments arranged by size:
4519 1413 72
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2101 3735 4693
Size: 416 53 83 399 24 684 1634 958
Cuts at: 4693 442
Size: 1753
Fragments arranged by size:
1753 1634 958 684 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3137
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2235 2337 2880 2999 4664 1239
Size: 996 102 543 119 1665 2579
Fragments arranged by size:
2579 1665 996 543 119 102
Bpu10I CC'TnA_GC
Cuts at: 3087 3087
Size: 6004
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4129 4994 5292 4129
Size: 865 298 4841
Fragments arranged by size:
4841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2501 2744 4667 882
Size: 617 1002 243 1923 2219
Fragments arranged by size:
2219 1923 1002 617 243
BsaAI yAC'GTr
Cuts at: 493 1204 2425 2685 3195 3223 3286 3795 493
Size: 711 1221 260 510 28 63 509 2702
Fragments arranged by size:
2702 1221 711 510 509 260 63 28
BsaBI GATnn'nnATC
Cuts at: 2289 5998 2289
Size: 3709 2295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3811 4193 4828 275
Size: 53 83 186 936 2278 382 635 1451
Fragments arranged by size:
2278 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3162 4437 4835 5109 5256 5667 3162
Size: 1275 398 274 147 411 3499
Fragments arranged by size:
3499 1275 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2310 2340 3170 3200 1178
Size: 30 165 30 907 30 830 30 3982
Fragments arranged by size:
3982 907 830 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2996 3017 1186
Size: 1810 21 4173
Fragments arranged by size:
4173 1810 21
BseYI C'CCAG_C
Cuts at: 2921 5207 5703 2921
Size: 2286 496 3222
Fragments arranged by size:
3222 2286 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2741 3140 1163
Size: 573 1005 399 4027
Fragments arranged by size:
4027 1005 573 399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4065 4150 5221 4065
Size: 85 1071 4848
Fragments arranged by size:
4848 1071 85
BsiWI C'GTAC_G
Cuts at: 3195 3195
Size: 6004
BsmI GAATG_Cn'
Cuts at: 3515 3608 3515
Size: 93 5911
BsmBI CGTCTCn'nnnn_
Cuts at: 3067 3067
Size: 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3118 3132 3323 4065 4150 5221 1391
Size: 399 1328 14 191 742 85 1071 2174
Fragments arranged by size:
2174 1328 1071 742 399 191 85 14
BspEI T'CCGG_A
Cuts at: 3162 5667 3162
Size: 2505 3499
BspHI T'CATG_A
Cuts at: 2946 3893 5623 2946
Size: 947 1730 3327
Fragments arranged by size:
3327 1730 947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2099 2604 3359 844
Size: 1255 505 755 3489
Fragments arranged by size:
3489 1255 755 505
BsrBI CCG'CTC
Cuts at: 1394 1823 3121 3891 1394
Size: 429 1298 770 3507
Fragments arranged by size:
3507 1298 770 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2185 2223 4499 4673 65
Size: 1183 937 38 2276 174 1396
Fragments arranged by size:
2276 1396 1183 937 174 38
BsrFI r'CCGG_y
Cuts at: 1700 1810 4648 4835 1700
Size: 110 2838 187 2869
Fragments arranged by size:
2869 2838 187 110
BsrGI T'GTAC_A
Cuts at: 96 2523 96
Size: 2427 3577
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2627 794
Size: 1833 4171
BstBI TT'CG_AA
Cuts at: 4808 4808
Size: 6004
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstXI CCAn_nnnn'nTGG
Cuts at: 2689 2977 2689
Size: 288 5716
BstYI r'GATC_y
Cuts at: 1972 2387 2450 2482 2737 3006 3334 3701 4086
Size: 415 63 32 255 269 328 367 385
Cuts at: 4086 4103 5555 5641 5653 5999 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 415 385 367 346 328 269
255 86 63 32 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2446 3276 513
Size: 552 939 442 830 3241
Fragments arranged by size:
3241 939 830 552 442
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2376 487
Size: 683 56 1150 4115
Fragments arranged by size:
4115 1150 683 56
BtsI GCAGTG_nn'
Cuts at: 2379 3169 3520 4314 4334 2379
Size: 790 351 794 20 4049
Fragments arranged by size:
4049 794 790 351 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5969
DraI TTT'AAA
Cuts at: 3308 3308
Size: 6004
DraIII CAC_nnn'GTG
Cuts at: 2095 2095
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 809
Size: 507 3695 1802
Fragments arranged by size:
3695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3451 4340 5969
Size: 54 1425 50 275 18 1621 889 1629
Cuts at: 5969 8
Size: 43
Fragments arranged by size:
1629 1621 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2399 2669 3934 1353
Size: 1046 270 1265 3423
Fragments arranged by size:
3423 1265 1046 270
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4591 4965 5111 387
Size: 1064 138 3002 374 146 1280
Fragments arranged by size:
3002 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2235 2256 2337 2423 2880 2999
Size: 393 462 141 21 81 86 457 119
Cuts at: 2999 4067 4664 5451 5719 1239
Size: 1068 597 787 268 1524
Fragments arranged by size:
1524 1068 787 597 462 457 393 268
141 119 86 81 21
EcoO109I rG'GnC_Cy
Cuts at: 2497 3148 2497
Size: 651 5353
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 5151 1678
Size: 3473 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2435 2467 2564 2596 2663
Size: 32 135 32 863 32 97 32 67
Cuts at: 2663 2695 4645 4677 4719 4751 1373
Size: 32 1950 32 42 32 2626
Fragments arranged by size:
2626 1950 863 135 97 67 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2710 3232 3348 3594 669
Size: 988 281 772 522 116 246 3079
Fragments arranged by size:
3079 988 772 522 281 246 116
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 3603 3603
Size: 6004
MluI A'CGCG_T
Cuts at: 2148 5880 2148
Size: 3732 2272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5118 5302 1305
Size: 3813 184 2007
Fragments arranged by size:
3813 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4182
Fragments arranged by size:
4182 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2178 2840 2945 3021 3962 4321 4480
Size: 648 1012 662 105 76 941 359 159
Cuts at: 4480 518
Size: 2042
Fragments arranged by size:
2042 1012 941 662 648 359 159 105
76
MspA1I CmG'CkG
Cuts at: 1606 3013 3135 3420 4097 5245 5490 1606
Size: 1407 122 285 677 1148 245 2120
Fragments arranged by size:
2120 1407 1148 677 285 245 122
NcoI C'CATG_G
Cuts at: 513 1065 2004 2446 3276 513
Size: 552 939 442 830 3241
Fragments arranged by size:
3241 939 830 552 442
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 2524 3362 4907 1132
Size: 97 1295 838 1545 2229
Fragments arranged by size:
2229 1545 1295 838 97
PasI CC'CwG_GG
Cuts at: 1348 2041 1348
Size: 693 5311
PciI A'CATG_T
Cuts at: 2520 4903 2520
Size: 2383 3621
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5102
PfoI T'CCnGG_A
Cuts at: 2251 2251
Size: 6004
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PmlI CAC'GTG
Cuts at: 2425 3286 2425
Size: 861 5143
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2497 2497
Size: 6004
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PstI C_TGCA'G
Cuts at: 830 3356 830
Size: 2526 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
PvuII CAG'CTG
Cuts at: 3135 3135
Size: 6004
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 4252 5964 4252
Size: 1712 4292
SexAI A'CCwGG_T
Cuts at: 2772 2772
Size: 6004
SfcI C'TryA_G
Cuts at: 826 1045 2061 3352 4487 5168 5359 826
Size: 219 1016 1291 1135 681 191 1471
Fragments arranged by size:
1471 1291 1135 1016 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 3331 1872
Size: 1459 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4108 5009 5271 820
Size: 197 926 2165 901 262 1553
Fragments arranged by size:
2165 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 3362 1132
Size: 2230 3774
SspI AAT'ATT
Cuts at: 5 52 3928 5
Size: 47 3876 2081
Fragments arranged by size:
3876 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6004
StyI C'CwwG_G
Cuts at: 513 1065 2004 2446 3276 3292 3443 513
Size: 552 939 442 830 16 151 3074
Fragments arranged by size:
3074 939 830 552 442 151 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2083 4031 4048 4201 4386 4831 325
Size: 1255 503 1948 17 153 185 445 1498
Fragments arranged by size:
1948 1498 1255 503 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2523 4250 5962
Size: 274 80 33 51 1443 546 1727 1712
Cuts at: 5962 96
Size: 138
Fragments arranged by size:
1727 1712 1443 546 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2325 2357 3272 3304 5964 5996 2325
Size: 32 915 32 2660 32 2333
Fragments arranged by size:
2660 2333 915 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6004
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2453 3290 2453
Size: 837 5167
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 3329 1870
Size: 1459 4545
XmnI GAAnn'nnTTC
Cuts at: 2484 4133 2484
Size: 1649 4355
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI