Restriction map for
pFN21AE3583
(Circular) (Six-base) MAPSORT of: pFN21AE3583.seq Check: 1969 from: 1 to: 5959
pFN21AE3583 5959 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2788 2788
Size: 5959
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4022 5406 5674 1632
Size: 2390 1384 268 1917
Fragments arranged by size:
2390 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2430 2477 1184
Size: 1246 47 4666
Fragments arranged by size:
4666 1246 47
AlwNI CAG_nnn'CTG
Cuts at: 3122 3702 5274 3122
Size: 580 1572 3807
Fragments arranged by size:
3807 1572 580
ApaI G_GGCC'C
Cuts at: 2099 2735 2099
Size: 636 5323
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2216 2999 3268 3494 3646 5755 1493
Size: 723 783 269 226 152 2109 1697
Fragments arranged by size:
2109 1697 783 723 269 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2601 3284 1870
Size: 73 658 683 4545
Fragments arranged by size:
4545 683 658 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2702 2735 2702
Size: 33 5926
BamHI G'GATC_C
Cuts at: 2865 2956 3289 3656 2865
Size: 91 333 367 5168
Fragments arranged by size:
5168 367 333 91
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3280 4735 618
Size: 325 217 627 1493 1455 1842
Fragments arranged by size:
1842 1493 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2099 2318 2553 2574 2702 2735 3278 1391
Size: 708 219 235 21 128 33 543 4072
Fragments arranged by size:
4072 708 543 235 219 128 33 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2645 3093 928
Size: 971 746 448 3794
Fragments arranged by size:
3794 971 746 448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2789 2823 4150 4184 1903
Size: 34 852 34 1327 34 3678
Fragments arranged by size:
3678 1327 852 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 2902 1548
Size: 1354 4605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2393 2628 2980
Size: 107 122 71 631 605 721 235 352
Cuts at: 2980 4570 136
Size: 1590 1525
Fragments arranged by size:
1590 1525 721 631 605 352 235 122
107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 2099 2735 4020 5176 1790
Size: 309 636 1285 1156 2573
Fragments arranged by size:
2573 1285 1156 636 309
BmgBI CAC'GTC
Cuts at: 1318 2428 1318
Size: 1110 4849
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3690 4648 442
Size: 416 53 83 399 24 2273 958 1753
Fragments arranged by size:
2273 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2627 2627
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2177 2186 2303 2894 4619 1239
Size: 896 42 9 117 591 1725 2579
Fragments arranged by size:
2579 1725 896 591 117 42 9
Bpu10I CC'TnA_GC
Cuts at: 2037 2100 2351 2037
Size: 63 251 5645
Fragments arranged by size:
5645 251 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 4084 4949 5247 2360
Size: 1724 865 298 3072
Fragments arranged by size:
3072 1724 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4622 882
Size: 617 3123 2219
Fragments arranged by size:
3123 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 2018 2588 5953 2018
Size: 570 3365 2024
Fragments arranged by size:
3365 2024 570
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2607 3766 4148 4783
Size: 53 83 186 936 1074 1159 382 635
Cuts at: 4783 275
Size: 1451
Fragments arranged by size:
1451 1159 1074 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2108 2138 3233 3263 1178
Size: 30 165 30 705 30 1095 30 3874
Fragments arranged by size:
3874 1095 705 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2249 3086 1186
Size: 838 225 837 4059
Fragments arranged by size:
4059 838 837 225
BseYI C'CCAG_C
Cuts at: 2050 5162 5658 2050
Size: 3112 496 2351
Fragments arranged by size:
3112 2351 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2192 1163
Size: 573 456 4930
Fragments arranged by size:
4930 573 456
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2365 3409 4170 5198 1540
Size: 275 186 364 1044 761 1028 2301
Fragments arranged by size:
2301 1044 1028 761 364 275 186
BsiHKAI G_wGCw'C
Cuts at: 2357 2553 2574 4020 4105 5176 2357
Size: 196 21 1446 85 1071 3140
Fragments arranged by size:
3140 1446 1071 196 85 21
BsmI GAATG_Cn'
Cuts at: 2681 2779 3470 3563 2681
Size: 98 691 93 5077
Fragments arranged by size:
5077 691 98 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 2318 2357 2553 2574 2702 2735
Size: 399 309 219 39 196 21 128 33
Cuts at: 2735 3278 4020 4105 5176 1391
Size: 543 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 543 399 309 219 196
128 85 39 33 21
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 2457 3848 5578 2457
Size: 1391 1730 2838
Fragments arranged by size:
2838 1730 1391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2788 3314 844
Size: 1944 526 3489
Fragments arranged by size:
3489 1944 526
BsrBI CCG'CTC
Cuts at: 1394 1823 2557 3846 1394
Size: 429 734 1289 3507
Fragments arranged by size:
3507 1289 734 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2419 4454 4628 65
Size: 1183 1171 2035 174 1396
Fragments arranged by size:
2035 1396 1183 1171 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2078 4603 4790 1700
Size: 110 268 2525 187 2869
Fragments arranged by size:
2869 2525 268 187 110
BsrGI T'GTAC_A
Cuts at: 96 2124 2710 96
Size: 2028 586 3345
Fragments arranged by size:
3345 2028 586
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2533 794
Size: 1739 4220
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2046 2865 2956 3289 3656 4041 4058 5510
Size: 74 819 91 333 367 385 17 1452
Cuts at: 5510 5596 5608 5954 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 819 385 367 346 333 91
86 74 17 12
Bsu36I CC'TnA_GG
Cuts at: 2106 2106
Size: 5959
BtgI C'CryG_G
Cuts at: 513 1065 2004 2544 513
Size: 552 939 540 3928
Fragments arranged by size:
3928 939 552 540
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2797 487
Size: 683 56 1571 3649
Fragments arranged by size:
3649 1571 683 56
BtsI GCAGTG_nn'
Cuts at: 3475 4269 4289 3475
Size: 794 20 5145
Fragments arranged by size:
5145 794 20
ClaI AT'CG_AT
Cuts at: 1554 2361 2592 1554
Size: 807 231 4921
Fragments arranged by size:
4921 807 231
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2640 2675 529
Size: 35 2076 35 3813
Fragments arranged by size:
3813 2076 35 35
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2083 4966 809
Size: 507 767 2883 1802
Fragments arranged by size:
2883 1802 767 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2763 2931 2972
Size: 54 1425 50 275 18 933 168 41
Cuts at: 2972 3101 3189 3406 4295 5924 8
Size: 129 88 217 889 1629 43
Fragments arranged by size:
1629 1425 933 889 275 217 168 129
88 54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3020 3889 1353
Size: 1667 869 3423
Fragments arranged by size:
3423 1667 869
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2747 4546 4920 5066 387
Size: 1064 138 1158 1799 374 146 1280
Fragments arranged by size:
1799 1280 1158 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2551 2572 2551
Size: 21 5938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2177 2186 2303 2894 4022 4619
Size: 393 503 42 9 117 591 1128 597
Cuts at: 4619 5406 5674 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1128 787 597 591 503 393 268
117 42 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2210 2765 3068 3191 2210
Size: 555 303 123 4978
Fragments arranged by size:
4978 555 303 123
EcoO109I rG'GnC_Cy
Cuts at: 2096 2110 2119 2731 2732 2096
Size: 14 9 612 1 5323
Fragments arranged by size:
5323 612 14 9 1
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FspI TGC'GCA
Cuts at: 2345 4465 2345
Size: 2120 3839
FspAI rTGC'GCAy
Cuts at: 2345 2345
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 5106 1678
Size: 3428 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3116 3148 4600 4632 4674
Size: 32 135 32 1544 32 1452 32 42
Cuts at: 4674 4706 1373
Size: 32 2626
Fragments arranged by size:
2626 1544 1452 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3079 3303 3549 669
Size: 988 281 1141 224 246 3079
Fragments arranged by size:
3079 1141 988 281 246 224
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2355 5073 5257 1305
Size: 1050 2718 184 2007
Fragments arranged by size:
2718 2007 1050 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2765 2933 3191 10
Size: 54 1425 343 933 168 258 2778
Fragments arranged by size:
2778 1425 933 343 258 168 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 2412 2459 3917 4276 4435 518
Size: 648 858 388 47 1458 359 159 2042
Fragments arranged by size:
2042 1458 858 648 388 359 159 47
MspA1I CmG'CkG
Cuts at: 1606 2263 2678 3375 4052 5200 5445 1606
Size: 657 415 697 677 1148 245 2120
Fragments arranged by size:
2120 1148 697 677 657 415 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4468
Fragments arranged by size:
4468 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3317 4862 1132
Size: 97 2088 1545 2229
Fragments arranged by size:
2229 2088 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PfoI T'CCnGG_A
Cuts at: 2163 2745 2163
Size: 582 5377
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2144 2176 4028 4060 5541 5573 2144
Size: 32 1852 32 1481 32 2530
Fragments arranged by size:
2530 1852 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2110 2119 2110
Size: 9 5950
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PspOMI G'GGCC_C
Cuts at: 2095 2731 2095
Size: 636 5323
PstI C_TGCA'G
Cuts at: 830 2148 3311 830
Size: 1318 1163 3478
Fragments arranged by size:
3478 1318 1163
PvuI CG_AT'CG
Cuts at: 2001 2365 2001
Size: 364 5595
PvuII CAG'CTG
Cuts at: 2678 2678
Size: 5959
SacI G_AGCT'C
Cuts at: 2553 2574 2553
Size: 21 5938
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2144 3307 4442 5123 5314 826
Size: 219 1099 1163 1135 681 191 1471
Fragments arranged by size:
1471 1163 1135 1099 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2375 4063 4964 5226 820
Size: 197 926 432 1688 901 262 1553
Fragments arranged by size:
1688 1553 926 901 432 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2004 2766 3192 3398 513
Size: 552 939 762 426 206 3074
Fragments arranged by size:
3074 939 762 552 426 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2714 3986 4003 4156 4341 4786 325
Size: 1255 1134 1272 17 153 185 445 1498
Fragments arranged by size:
1498 1272 1255 1134 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 2710 4205
Size: 274 80 33 51 1443 147 586 1495
Cuts at: 4205 5917 96
Size: 1712 138
Fragments arranged by size:
1712 1495 1443 586 274 147 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2941 2973 5919 5951 2941
Size: 32 2946 32 2949
Fragments arranged by size:
2949 2946 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 2389
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI