Restriction map for
pFN21AE3581
(Circular) (Six-base) MAPSORT of: pFN21AE3581.seq Check: 612 from: 1 to: 7948
pFN21AE3581 7948 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2527 3388 2527
Size: 861 7087
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3803 5096 5758 278
Size: 53 83 186 936 2267 1293 662 2468
Fragments arranged by size:
2468 2267 1293 936 662 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3426 4658 5291 1937
Size: 1489 1232 633 4594
Fragments arranged by size:
4594 1489 1232 633
Acc65I G'GTAC_C
Cuts at: 5269 5269
Size: 7948
AclI AA'CG_TT
Cuts at: 1563 6075 6448 1563
Size: 4512 373 3063
Fragments arranged by size:
4512 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2560 2849 2939 3044 3182 4577 6011 7395
Size: 928 289 90 105 138 1395 1434 1384
Cuts at: 7395 7663 1632
Size: 268 1917
Fragments arranged by size:
1917 1434 1395 1384 928 289 268 138
105 90
AfeI AGC'GCT
Cuts at: 2665 2762 2665
Size: 97 7851
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7751
AflIII A'CryG_T
Cuts at: 1203 2139 4578 6847 7824 1203
Size: 936 2439 2269 977 1327
Fragments arranged by size:
2439 2269 1327 977 936
AgeI A'CCGG_T
Cuts at: 6779 6779
Size: 7948
AhdI GACnn_n'nnGTC
Cuts at: 2227 6677 2227
Size: 4450 3498
AleI CACnn'nnGTG
Cuts at: 1166 2238 2333 2681 1166
Size: 1072 95 348 6433
Fragments arranged by size:
6433 1072 348 95
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7948
AlwNI CAG_nnn'CTG
Cuts at: 2169 2481 3160 3775 4848 5691 7263 2169
Size: 312 679 615 1073 843 1572 2854
Fragments arranged by size:
2854 1572 1073 843 679 615 312
ApaLI G'TGCA_C
Cuts at: 2696 6005 7161 2696
Size: 3309 1156 3483
Fragments arranged by size:
3483 3309 1156
ApoI r'AATT_y
Cuts at: 1493 5257 5483 5635 7744 1493
Size: 3764 226 152 2109 1697
Fragments arranged by size:
3764 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6502 160
Size: 872 5470 1606
Fragments arranged by size:
5470 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7948
AvaI C'yCGr_G
Cuts at: 1870 1943 5102 5273 1870
Size: 73 3159 171 4545
Fragments arranged by size:
4545 3159 171 73
BamHI G'GATC_C
Cuts at: 5278 5645 5278
Size: 367 7581
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2505 5269 6724 618
Size: 325 217 627 239 479 2764 1455 1842
Fragments arranged by size:
2764 1842 1455 627 479 325 239 217
BanII G_rGCy'C
Cuts at: 1391 2127 2160 2568 2994 3185 3207 3701 4052
Size: 736 33 408 426 191 22 494 351
Cuts at: 4052 4790 5267 1391
Size: 738 477 4072
Fragments arranged by size:
4072 738 736 494 477 426 408 351
191 33 22
BbeI G_GCGC'C
Cuts at: 2030 2030
Size: 7948
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2331 2817 4921 5115 5217 928
Size: 971 432 486 2104 194 102 3659
Fragments arranged by size:
3659 2104 971 486 432 194 102
BbvCI CC'TCA_GC
Cuts at: 4650 4650
Size: 7948
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3934 3968 6139 6173 1903
Size: 34 50 34 1913 34 2171 34 3678
Fragments arranged by size:
3678 2171 1913 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5840 7050 5840
Size: 1210 6738
BclI T'GATC_A
Cuts at: 1548 2886 1548
Size: 1338 6610
BfrBI ATG'CAT
Cuts at: 3128 4804 3128
Size: 1676 6272
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4292 6559 136
Size: 107 122 71 631 605 2620 2267 1525
Fragments arranged by size:
2620 2267 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7943 7943
Size: 7948
BlpI GC'TnA_GC
Cuts at: 4059 5365 4059
Size: 1306 6642
Bme1580I G_kGCm'C
Cuts at: 1790 2147 2700 3342 6009 7165 1790
Size: 357 553 642 2667 1156 2573
Fragments arranged by size:
2667 2573 1156 642 553 357
BmgBI CAC'GTC
Cuts at: 1318 3363 4354 4437 4581 1318
Size: 2045 991 83 144 4685
Fragments arranged by size:
4685 2045 991 144 83
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4723 5679 6637
Size: 416 53 83 399 24 3306 956 958
Cuts at: 6637 442
Size: 1753
Fragments arranged by size:
3306 1753 958 956 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7948
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3769 3769
Size: 7948
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 3002 3008 4661 4706 4795 6608 1239
Size: 1130 633 6 1653 45 89 1813 2579
Fragments arranged by size:
2579 1813 1653 1130 633 89 45 6
Bpu10I CC'TnA_GC
Cuts at: 3459 4650 4784 5183 3459
Size: 1191 134 399 6224
Fragments arranged by size:
6224 1191 399 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6073 6938 7236 6073
Size: 865 298 6785
Fragments arranged by size:
6785 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2237 2291 2732 3784 6611 882
Size: 617 738 54 441 1052 2827 2219
Fragments arranged by size:
2827 2219 1052 738 617 441 54
BsaAI yAC'GTr
Cuts at: 493 1204 4974 5739 493
Size: 711 3770 765 2702
Fragments arranged by size:
3770 2702 765 711
BsaBI GATnn'nnATC
Cuts at: 4805 7942 4805
Size: 3137 4811
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 2814 3287 3800
Size: 53 83 186 936 494 787 473 513
Cuts at: 3800 4661 5093 5755 6137 6772 275
Size: 861 432 662 382 635 1451
Fragments arranged by size:
1451 936 861 787 662 635 513 494
473 432 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5206 6381 6779 7053 7200 7611 5206
Size: 1175 398 274 147 411 5543
Fragments arranged by size:
5543 1175 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2171 2201 2366 2396 2411
Size: 30 165 30 768 30 165 30 15
Cuts at: 2411 2441 1178
Size: 30 6685
Fragments arranged by size:
6685 768 165 165 30 30 30 30
30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2117 2309 2419 3503 3851 3893 4447
Size: 840 91 192 110 1084 348 42 554
Cuts at: 4447 4658 4667 1186
Size: 211 9 4467
Fragments arranged by size:
4467 1084 840 554 348 211 192 110
91 42 9
BseYI C'CCAG_C
Cuts at: 2174 2567 2996 3633 3647 3788 4016 4704 7151
Size: 393 429 637 14 141 228 688 2447
Cuts at: 7151 7647 2174
Size: 496 2475
Fragments arranged by size:
2475 2447 688 637 496 429 393 228
141 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2402 2606 1163
Size: 573 666 204 6505
Fragments arranged by size:
6505 666 573 204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2196 4339 5398 6159 7187 1540
Size: 275 186 195 2143 1059 761 1028 2301
Fragments arranged by size:
2301 2143 1059 1028 761 275 195 186
BsiHKAI G_wGCw'C
Cuts at: 2160 2700 2994 3185 3207 6009 6094 7165 2160
Size: 540 294 191 22 2802 85 1071 2943
Fragments arranged by size:
2943 2802 1071 540 294 191 85 22
BsmI GAATG_Cn'
Cuts at: 4534 5459 5552 4534
Size: 925 93 6930
Fragments arranged by size:
6930 925 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2702 3278 2702
Size: 576 7372
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2147 2160 2568 2700 2994 3185
Size: 399 337 20 13 408 132 294 191
Cuts at: 3185 3207 3342 3701 4052 4790 5267 6009 6094
Size: 22 135 359 351 738 477 742 85
Cuts at: 6094 7165 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 738 477 408 399 359
351 337 294 191 135 132 85 22
20 13
BspEI T'CCGG_A
Cuts at: 5206 7611 5206
Size: 2405 5543
BspHI T'CATG_A
Cuts at: 4188 5837 7567 4188
Size: 1649 1730 4569
Fragments arranged by size:
4569 1730 1649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2424 2527 3388 5236 5303 844
Size: 1580 103 861 1848 67 3489
Fragments arranged by size:
3489 1848 1580 861 103 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4798 5835 1394
Size: 429 2975 1037 3507
Fragments arranged by size:
3507 2975 1037 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6443 6617 65
Size: 1183 5195 174 1396
Fragments arranged by size:
5195 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2595 4063 6592 6779 1700
Size: 110 785 1468 2529 187 2869
Fragments arranged by size:
2869 2529 1468 785 187 110
BsrGI T'GTAC_A
Cuts at: 96 2367 96
Size: 2271 5677
BssHII G'CGCG_C
Cuts at: 1925 2035 1925
Size: 110 7838
BssSI C'ACGA_G
Cuts at: 6008 7020 6008
Size: 1012 6936
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5139 794
Size: 4345 3603
BstBI TT'CG_AA
Cuts at: 4876 6752 4876
Size: 1876 6072
BstEII G'GTnAC_C
Cuts at: 1184 3244 1184
Size: 2060 5888
BstXI CCAn_nnnn'nTGG
Cuts at: 3113 3970 3113
Size: 857 7091
BstYI r'GATC_y
Cuts at: 1972 3156 5278 5645 6030 6047 7499 7585 7597
Size: 1184 2122 367 385 17 1452 86 12
Cuts at: 7597 7943 1972
Size: 346 1977
Fragments arranged by size:
2122 1977 1452 1184 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2517 3831 4025 4464 513
Size: 552 1452 1314 194 439 3997
Fragments arranged by size:
3997 1452 1314 552 439 194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3999 487
Size: 683 56 2773 4436
Fragments arranged by size:
4436 2773 683 56
BtsI GCAGTG_nn'
Cuts at: 3207 5464 6258 6278 3207
Size: 2257 794 20 4877
Fragments arranged by size:
4877 2257 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7948
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7913
DraI TTT'AAA
Cuts at: 5252 5252
Size: 7948
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6955 809
Size: 507 5639 1802
Fragments arranged by size:
5639 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2193 2845
Size: 54 1425 50 275 18 300 63 652
Cuts at: 2845 3103 3229 4122 4293 4854 4918 5395 6284
Size: 258 126 893 171 561 64 477 889
Cuts at: 6284 7913 8
Size: 1629 43
Fragments arranged by size:
1629 1425 893 889 652 561 477 300
275 258 171 126 64 63 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2193 5395 1537
Size: 275 381 3202 4090
Fragments arranged by size:
4090 3202 381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2286 2579 3480 3511 3537 3704 4385
Size: 699 234 293 901 31 26 167 681
Cuts at: 4385 4667 5878 1353
Size: 282 1211 3423
Fragments arranged by size:
3423 1211 901 699 681 293 282 234
167 31 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4292 6535 6909 7055 387
Size: 1064 138 2703 2243 374 146 1280
Fragments arranged by size:
2703 2243 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2158 2992 3183 3205 2158
Size: 834 191 22 6901
Fragments arranged by size:
6901 834 191 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 2560 2849 2939 3002 3008 3044
Size: 393 737 191 289 90 63 6 36
Cuts at: 3044 3182 4577 4661 4706 4795 6011 6608 7395
Size: 138 1395 84 45 89 1216 597 787
Cuts at: 7395 7663 1239
Size: 268 1524
Fragments arranged by size:
1524 1395 1216 787 737 597 393 289
268 191 138 90 89 84 63 45
36 6
EcoNI CCTnn'n_nnAGG
Cuts at: 4205 4205
Size: 7948
EcoO109I rG'GnC_Cy
Cuts at: 2166 2166
Size: 7948
EcoRI G'AATT_C
Cuts at: 5257 5635 5257
Size: 378 7570
EcoRV GAT'ATC
Cuts at: 3568 3568
Size: 7948
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3838 5234 3838
Size: 1396 6552
FspI TGC'GCA
Cuts at: 6454 6454
Size: 7948
HaeII r_GCGC'y
Cuts at: 1678 2030 2517 2667 2764 4176 7095 1678
Size: 352 487 150 97 1412 2919 2531
Fragments arranged by size:
2919 2531 1412 487 352 150 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 4934 4966 5035
Size: 32 135 32 641 32 2689 32 69
Cuts at: 5035 5067 5110 5142 6589 6621 6663 6695 1373
Size: 32 43 32 1447 32 42 32 2626
Fragments arranged by size:
2689 2626 1447 641 135 69 43 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2691 3427 4162 4357 4659 5292
Size: 988 281 753 736 735 195 302 633
Cuts at: 5292 5538 669
Size: 246 3079
Fragments arranged by size:
3079 988 753 736 735 633 302 281
246 195
HindIII A'AGCT_T
Cuts at: 748 2973 3905 748
Size: 2225 932 4791
Fragments arranged by size:
4791 2225 932
HpaI GTT'AAC
Cuts at: 4162 5538 4162
Size: 1376 6572
KasI G'GCGC_C
Cuts at: 2026 2026
Size: 7948
KpnI G_GTAC'C
Cuts at: 5273 5273
Size: 7948
MfeI C'AATT_G
Cuts at: 5547 5547
Size: 7948
MluI A'CGCG_T
Cuts at: 7824 7824
Size: 7948
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2319 2409 2424 3096 7062 7246 1305
Size: 1014 90 15 672 3966 184 2007
Fragments arranged by size:
3966 2007 1014 672 184 90 15
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2847 3231 4295 4856 4920
Size: 54 1425 343 1015 384 1064 561 64
Cuts at: 4920 10
Size: 3038
Fragments arranged by size:
3038 1425 1064 1015 561 384 343 64
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2238 2333 2681 3111 3968 4247 5906
Size: 648 1072 95 348 430 857 279 1659
Cuts at: 5906 6265 6424 518
Size: 359 159 2042
Fragments arranged by size:
2042 1659 1072 857 648 430 359 348
279 159 95
MspA1I CmG'CkG
Cuts at: 1606 2481 2490 2590 2649 4027 4289 5364 6041
Size: 875 9 100 59 1378 262 1075 677
Cuts at: 6041 7189 7434 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1378 1148 1075 875 677 262 245
100 59 9
NarI GG'CG_CC
Cuts at: 2027 2027
Size: 7948
NcoI C'CATG_G
Cuts at: 513 1065 2517 3831 513
Size: 552 1452 1314 4630
Fragments arranged by size:
4630 1452 1314 552
NdeI CA'TA_TG
Cuts at: 387 2883 387
Size: 2496 5452
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7948
NotI GC'GGCC_GC
Cuts at: 5395 5395
Size: 7948
NsiI A_TGCA'T
Cuts at: 3130 4806 3130
Size: 1676 6272
NspI r_CATG'y
Cuts at: 1132 1229 2143 2557 2839 4252 5306 6851 1132
Size: 97 914 414 282 1413 1054 1545 2229
Fragments arranged by size:
2229 1545 1413 1054 914 414 282 97
PasI CC'CwG_GG
Cuts at: 1348 3694 1348
Size: 2346 5602
PciI A'CATG_T
Cuts at: 2139 6847 2139
Size: 4708 3240
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2218 2763 3220 3775 4852 1108
Size: 1110 545 457 555 1077 4204
Fragments arranged by size:
4204 1110 1077 555 545 457
PfoI T'CCnGG_A
Cuts at: 2559 2559
Size: 7948
PmeI GTTT'AAAC
Cuts at: 5252 5252
Size: 7948
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6017 6049 7530 7562 6017
Size: 32 1481 32 6403
Fragments arranged by size:
6403 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3290 3776 1613
Size: 1677 486 5785
Fragments arranged by size:
5785 1677 486
PsiI TTA'TAA
Cuts at: 5518 5518
Size: 7948
PstI C_TGCA'G
Cuts at: 830 2535 2663 2868 3201 4182 4314 5300 830
Size: 1705 128 205 333 981 132 986 3478
Fragments arranged by size:
3478 1705 986 981 333 205 132 128
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7948
PvuII CAG'CTG
Cuts at: 2481 2590 2481
Size: 109 7839
SacI G_AGCT'C
Cuts at: 2160 2994 3185 3207 2160
Size: 834 191 22 6901
Fragments arranged by size:
6901 834 191 22
SacII CC_GC'GG
Cuts at: 4028 4028
Size: 7948
SalI G'TCGA_C
Cuts at: 1936 3425 4657 5290 1936
Size: 1489 1232 633 4594
Fragments arranged by size:
4594 1489 1232 633
SbfI CC_TGCA'GG
Cuts at: 2868 3201 5300 2868
Size: 333 2099 5516
Fragments arranged by size:
5516 2099 333
ScaI AGT'ACT
Cuts at: 6196 7908 6196
Size: 1712 6236
SfcI C'TryA_G
Cuts at: 826 1045 2531 2659 2864 3197 4178 4310 5296
Size: 219 1486 128 205 333 981 132 986
Cuts at: 5296 6431 7112 7303 826
Size: 1135 681 191 1471
Fragments arranged by size:
1486 1471 1135 986 981 681 333 219
205 191 132 128
SfiI GGCCn_nnn'nGGCC
Cuts at: 4292 4292
Size: 7948
SfoI GGC'GCC
Cuts at: 2028 2028
Size: 7948
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7948
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7948
SmaI CCC'GGG
Cuts at: 1872 5275 1872
Size: 3403 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6052 6953 7215 820
Size: 197 926 4109 901 262 1553
Fragments arranged by size:
4109 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5739 493
Size: 5246 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7948
SphI G_CATG'C
Cuts at: 1132 2557 4252 5306 1132
Size: 1425 1695 1054 3774
Fragments arranged by size:
3774 1695 1425 1054
SspI AAT'ATT
Cuts at: 5 52 5872 5
Size: 47 5820 2081
Fragments arranged by size:
5820 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7948
StyI C'CwwG_G
Cuts at: 513 1065 2301 2517 3552 3579 3831 4410 5387
Size: 552 1236 216 1035 27 252 579 977
Cuts at: 5387 513
Size: 3074
Fragments arranged by size:
3074 1236 1035 977 579 552 252 216
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2268 2756 3802 4023 4447 5975 5992
Size: 1255 688 488 1046 221 424 1528 17
Cuts at: 5992 6145 6330 6775 325
Size: 153 185 445 1498
Fragments arranged by size:
1528 1498 1255 1046 688 488 445 424
221 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 2367 2838
Size: 274 80 33 51 1443 159 231 471
Cuts at: 2838 4767 6194 7906 96
Size: 1929 1427 1712 138
Fragments arranged by size:
1929 1712 1443 1427 471 274 231 159
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2411 2443 7908 7940 2411
Size: 32 5465 32 2419
Fragments arranged by size:
5465 2419 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2335 1316
Size: 1019 6929
XbaI T'CTAG_A
Cuts at: 5284 5284
Size: 7948
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2254 3838 2254
Size: 1584 6364
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7948
XmaI C'CCGG_G
Cuts at: 1870 5273 1870
Size: 3403 4545
XmnI GAAnn'nnTTC
Cuts at: 6077 6077
Size: 7948
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3801 5094 5756 276
Size: 53 83 186 936 2267 1293 662 2468
Fragments arranged by size:
2468 2267 1293 936 662 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BsiWI BstZ17I Bsu36I
DraIII FseI FspAI NaeI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SexAI
SrfI SwaI