Restriction map for
pFN21AE3580
(Circular) (Six-base) MAPSORT of: pFN21AE3580.seq Check: 9255 from: 1 to: 7702
pFN21AE3580 7702 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2155 3499 4874 2155
Size: 1344 1375 4983
Fragments arranged by size:
4983 1375 1344
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3807 5512 278
Size: 53 83 186 936 2271 1705 2468
Fragments arranged by size:
2468 2271 1705 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5045 1937
Size: 3108 4594
Acc65I G'GTAC_C
Cuts at: 2753 5023 2753
Size: 2270 5432
AclI AA'CG_TT
Cuts at: 1563 5829 6202 1563
Size: 4266 373 3063
Fragments arranged by size:
4266 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3280 4478 5765 7149 7417 1632
Size: 1648 1198 1287 1384 268 1917
Fragments arranged by size:
1917 1648 1384 1287 1198 268
AfeI AGC'GCT
Cuts at: 4511 4511
Size: 7702
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7505
AflIII A'CryG_T
Cuts at: 1203 6601 7578 1203
Size: 5398 977 1327
Fragments arranged by size:
5398 1327 977
AgeI A'CCGG_T
Cuts at: 6533 6533
Size: 7702
AhdI GACnn_n'nnGTC
Cuts at: 6431 6431
Size: 7702
AleI CACnn'nnGTG
Cuts at: 1166 2243 2762 3680 3896 1166
Size: 1077 519 918 216 4972
Fragments arranged by size:
4972 1077 918 519 216
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3567 3755 4074 4772 1184
Size: 2383 188 319 698 4114
Fragments arranged by size:
4114 2383 698 319 188
AlwNI CAG_nnn'CTG
Cuts at: 3278 4279 4330 4953 5445 7017 3278
Size: 1001 51 623 492 1572 3963
Fragments arranged by size:
3963 1572 1001 623 492 51
ApaI G_GGCC'C
Cuts at: 3412 3784 4320 4553 4617 3412
Size: 372 536 233 64 6497
Fragments arranged by size:
6497 536 372 233 64
ApaLI G'TGCA_C
Cuts at: 5759 6915 5759
Size: 1156 6546
ApoI r'AATT_y
Cuts at: 1493 5011 5237 5389 7498 1493
Size: 3518 226 152 2109 1697
Fragments arranged by size:
3518 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6256 160
Size: 872 5224 1606
Fragments arranged by size:
5224 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7702
AvaI C'yCGr_G
Cuts at: 1870 1943 2118 2584 3092 3326 3363 3433 3474
Size: 73 175 466 508 234 37 70 41
Cuts at: 3474 3668 3999 4348 4546 4565 4616 5027 1870
Size: 194 331 349 198 19 51 411 4545
Fragments arranged by size:
4545 508 466 411 349 331 234 198
194 175 73 70 51 41 37 19
BamHI G'GATC_C
Cuts at: 2589 5032 5399 2589
Size: 2443 367 4892
Fragments arranged by size:
4892 2443 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2391 2753 3017 3498 3699
Size: 325 217 627 604 362 264 481 201
Cuts at: 3699 3898 4186 4415 4601 4731 5023 6478 618
Size: 199 288 229 186 130 292 1455 1842
Fragments arranged by size:
1842 1455 627 604 481 362 325 292
288 264 229 217 201 199 186 130
BanII G_rGCy'C
Cuts at: 1391 2514 2654 3412 3481 3784 4320 4454 4553
Size: 1123 140 758 69 303 536 134 99
Cuts at: 4553 4617 5021 1391
Size: 64 404 4072
Fragments arranged by size:
4072 1123 758 536 404 303 140 134
99 69 64
BbeI G_GCGC'C
Cuts at: 3502 4419 4605 3502
Size: 917 186 6599
Fragments arranged by size:
6599 917 186
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6731
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2275 2309 2608 2642 3317 3351 5893
Size: 34 338 34 299 34 675 34 2542
Cuts at: 5893 5927 1903
Size: 34 3678
Fragments arranged by size:
3678 2542 675 338 299 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5594 6804 5594
Size: 1210 6492
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7702
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2093 2262 2976
Size: 107 122 71 631 605 421 169 714
Cuts at: 2976 3854 4359 4767 6313 136
Size: 878 505 408 1546 1525
Fragments arranged by size:
1546 1525 878 714 631 605 505 421
408 169 122 107 71
BglII A'GATC_T
Cuts at: 4992 7697 4992
Size: 2705 4997
BlpI GC'TnA_GC
Cuts at: 4946 5119 4946
Size: 173 7529
Bme1580I G_kGCm'C
Cuts at: 1790 2240 2315 3020 3412 3784 3863 4320 4553
Size: 450 75 705 392 372 79 457 233
Cuts at: 4553 4617 5763 6919 1790
Size: 64 1146 1156 2573
Fragments arranged by size:
2573 1156 1146 705 457 450 392 372
233 79 75 64
BmgBI CAC'GTC
Cuts at: 1318 2742 3049 3133 3349 4117 4423 1318
Size: 1424 307 84 216 768 306 4597
Fragments arranged by size:
4597 1424 768 307 306 216 84
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2650 5433 6391
Size: 416 53 83 399 24 1233 2783 958
Cuts at: 6391 442
Size: 1753
Fragments arranged by size:
2783 1753 1233 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3980 1056
Size: 2924 4778
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 7702
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3788 3885 4430 6362 1239
Size: 2549 97 545 1932 2579
Fragments arranged by size:
2579 2549 1932 545 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2783 3947 4469 5827 6692 6990 2063
Size: 720 1164 522 1358 865 298 2775
Fragments arranged by size:
2775 1358 1164 865 720 522 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2531 6365 882
Size: 617 1032 3834 2219
Fragments arranged by size:
3834 2219 1032 617
BsaAI yAC'GTr
Cuts at: 493 1204 3745 4177 4255 4597 5493 493
Size: 711 2541 432 78 342 896 2702
Fragments arranged by size:
2702 2541 896 711 432 342 78
BsaBI GATnn'nnATC
Cuts at: 4094 7696 4094
Size: 3602 4100
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2385 3499 3711 3804
Size: 53 83 186 936 852 1114 212 93
Cuts at: 3804 3819 4020 4416 4602 5509 5891 6526 275
Size: 15 201 396 186 907 382 635 1451
Fragments arranged by size:
1451 1114 936 907 852 635 396 382
212 201 186 186 93 83 53 15
BsaWI w'CCGG_w
Cuts at: 4131 6135 6533 6807 6954 7365 4131
Size: 2004 398 274 147 411 4468
Fragments arranged by size:
4468 2004 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2607 2637 3941 3971 1178
Size: 30 165 30 1204 30 1304 30 4909
Fragments arranged by size:
4909 1304 1204 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3344 4247 4712 4838 1186
Size: 2158 903 465 126 4050
Fragments arranged by size:
4050 2158 903 465 126
BseYI C'CCAG_C
Cuts at: 2018 2703 3179 3460 3695 4205 4506 6905 7401
Size: 685 476 281 235 510 301 2399 496
Cuts at: 7401 2018
Size: 2319
Fragments arranged by size:
2399 2319 685 510 496 476 301 281
235
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2183 2318 2464 3077 3626 3725 3773
Size: 573 447 135 146 613 549 99 48
Cuts at: 3773 3824 3932 4231 4235 4577 1163
Size: 51 108 299 4 342 4288
Fragments arranged by size:
4288 613 573 549 447 342 299 146
135 108 99 51 48 4
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2108 2284 2530 4392 4447 4984
Size: 275 186 107 176 246 1862 55 537
Cuts at: 4984 5152 5913 6941 1540
Size: 168 761 1028 2301
Fragments arranged by size:
2301 1862 1028 761 537 275 246 186
176 168 107 55
BsiHKAI G_wGCw'C
Cuts at: 2514 3432 3687 3734 4485 4643 5763 5848 6919
Size: 918 255 47 751 158 1120 85 1071
Cuts at: 6919 2514
Size: 3297
Fragments arranged by size:
3297 1120 1071 918 751 255 158 85
47
BsmI GAATG_Cn'
Cuts at: 5213 5306 5213
Size: 93 7609
BsmBI CGTCTCn'nnnn_
Cuts at: 3065 3246 3065
Size: 181 7521
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 2315 2514 2654 3020 3412 3432
Size: 399 450 75 199 140 366 392 20
Cuts at: 3432 3481 3687 3734 3784 3863 4320 4454 4485
Size: 49 206 47 50 79 457 134 31
Cuts at: 4485 4553 4617 4643 5021 5763 5848 6919 1391
Size: 68 64 26 378 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 457 450 399 392 378
366 206 199 140 134 85 79 75
68 64 50 49 47 31 26 20
BspEI T'CCGG_A
Cuts at: 7365 7365
Size: 7702
BspHI T'CATG_A
Cuts at: 5591 7321 5591
Size: 1730 5972
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2155 2364 3270 3499 3621 3819 4599 4874
Size: 1311 209 906 229 122 198 780 275
Cuts at: 4874 5057 844
Size: 183 3489
Fragments arranged by size:
3489 1311 906 780 275 229 209 198
183 122
BsrBI CCG'CTC
Cuts at: 1394 1823 2543 3539 4522 5589 1394
Size: 429 720 996 983 1067 3507
Fragments arranged by size:
3507 1067 996 983 720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6197 6371 65
Size: 1183 4949 174 1396
Fragments arranged by size:
4949 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2333 2863 3321 3869 4842 6346 6533
Size: 110 523 530 458 548 973 1504 187
Cuts at: 6533 1700
Size: 2869
Fragments arranged by size:
2869 1504 973 548 530 523 458 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7702
BssHII G'CGCG_C
Cuts at: 1925 2346 3358 4704 4706 1925
Size: 421 1012 1346 2 4921
Fragments arranged by size:
4921 1346 1012 421 2
BssSI C'ACGA_G
Cuts at: 3068 4898 5762 6774 3068
Size: 1830 864 1012 3996
Fragments arranged by size:
3996 1830 1012 864
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2356 2431 2487 4731 4866 794
Size: 1562 75 56 2244 135 3630
Fragments arranged by size:
3630 2244 1562 135 75 56
BstBI TT'CG_AA
Cuts at: 6506 6506
Size: 7702
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7702
BstYI r'GATC_y
Cuts at: 1972 2589 4089 4992 5032 5399 5784 5801 7253
Size: 617 1500 903 40 367 385 17 1452
Cuts at: 7253 7339 7351 7697 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1500 1452 903 617 385 367 346
86 40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2260 2698 2971 3040 4441 4915
Size: 552 939 256 438 273 69 1401 474
Cuts at: 4915 513
Size: 3300
Fragments arranged by size:
3300 1401 939 552 474 438 273 256
69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6963
Fragments arranged by size:
6963 683 56
BtsI GCAGTG_nn'
Cuts at: 2306 2492 3638 3737 4219 5218 6012 6032 2306
Size: 186 1146 99 482 999 794 20 3976
Fragments arranged by size:
3976 1146 999 794 482 186 99 20
ClaI AT'CG_AT
Cuts at: 1554 3909 1554
Size: 2355 5347
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7667
DraI TTT'AAA
Cuts at: 5006 5006
Size: 7702
DraIII CAC_nnn'GTG
Cuts at: 2487 4692 2487
Size: 2205 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2945 6709 809
Size: 507 1629 3764 1802
Fragments arranged by size:
3764 1802 1629 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2105 2254 2730
Size: 54 1425 50 275 18 275 149 476
Cuts at: 2730 3351 3747 4464 4912 5149 6038 7667 8
Size: 621 396 717 448 237 889 1629 43
Fragments arranged by size:
1629 1425 889 717 621 476 448 396
275 275 237 149 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2105 5149 1537
Size: 275 293 3044 4090
Fragments arranged by size:
4090 3044 293 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3410 5632 1353
Size: 2057 2222 3423
Fragments arranged by size:
3423 2222 2057
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2882 3011 3722 3835 4883 6289
Size: 1064 138 1293 129 711 113 1048 1406
Cuts at: 6289 6663 6809 387
Size: 374 146 1280
Fragments arranged by size:
1406 1293 1280 1064 1048 711 374 146
138 129 113
EcoICRI GAG'CTC
Cuts at: 2512 2512
Size: 7702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3280 3788 3885 4430 4478 5765 6362
Size: 393 1648 508 97 545 48 1287 597
Cuts at: 6362 7149 7417 1239
Size: 787 268 1524
Fragments arranged by size:
1648 1524 1287 787 597 545 508 393
268 97 48
EcoNI CCTnn'n_nnAGG
Cuts at: 3563 3893 3563
Size: 330 7372
EcoO109I rG'GnC_Cy
Cuts at: 2115 2578 2633 3781 3886 4309 4316 4317 4489
Size: 463 55 1148 105 423 7 1 172
Cuts at: 4489 4550 4569 4613 2115
Size: 61 19 44 5204
Fragments arranged by size:
5204 1148 463 423 172 105 61 55
44 19 7 1
EcoRI G'AATT_C
Cuts at: 5011 5389 5011
Size: 378 7324
FspI TGC'GCA
Cuts at: 4127 6208 4127
Size: 2081 5621
HaeII r_GCGC'y
Cuts at: 1678 3101 3461 3502 4049 4262 4409 4419 4513
Size: 1423 360 41 547 213 147 10 94
Cuts at: 4513 4605 4655 4788 4953 6849 1678
Size: 92 50 133 165 1896 2531
Fragments arranged by size:
2531 1896 1423 547 360 213 165 147
133 94 92 50 41 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3809 3841 3982 4014 6343
Size: 32 135 32 2237 32 141 32 2329
Cuts at: 6343 6375 6417 6449 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2329 2237 141 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3808 4219 5046 5292 669
Size: 988 281 1870 411 827 246 3079
Fragments arranged by size:
3079 1870 988 827 411 281 246
HindIII A'AGCT_T
Cuts at: 748 3401 748
Size: 2653 5049
HpaI GTT'AAC
Cuts at: 5292 5292
Size: 7702
KasI G'GCGC_C
Cuts at: 3498 4415 4601 3498
Size: 917 186 6599
Fragments arranged by size:
6599 917 186
KpnI G_GTAC'C
Cuts at: 2757 5027 2757
Size: 2270 5432
MfeI C'AATT_G
Cuts at: 5301 5301
Size: 7702
MluI A'CGCG_T
Cuts at: 7578 7578
Size: 7702
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1991 4427 6816 7000 1305
Size: 686 2436 2389 184 2007
Fragments arranged by size:
2436 2389 2007 686 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2732 10
Size: 54 1425 343 900 4980
Fragments arranged by size:
4980 1425 900 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2243 2762 3680 3896 5660 6019
Size: 648 1011 66 519 918 216 1764 359
Cuts at: 6019 6178 518
Size: 159 2042
Fragments arranged by size:
2042 1764 1011 918 648 519 359 216
159 66
MspA1I CmG'CkG
Cuts at: 1606 2819 3042 3286 4012 4110 4150 4276 4327
Size: 1213 223 244 726 98 40 126 51
Cuts at: 4327 4443 4456 4625 4816 5118 5795 6943 7188
Size: 116 13 169 191 302 677 1148 245
Cuts at: 7188 1606
Size: 2120
Fragments arranged by size:
2120 1213 1148 726 677 302 245 244
223 191 169 126 116 98 51 40
13
NaeI GCC'GGC
Cuts at: 2335 2865 3871 2335
Size: 530 1006 6166
Fragments arranged by size:
6166 1006 530
NarI GG'CG_CC
Cuts at: 3499 4416 4602 3499
Size: 917 186 6599
Fragments arranged by size:
6599 917 186
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6211
Fragments arranged by size:
6211 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7702
NgoMIV G'CCGG_C
Cuts at: 2333 2863 3869 2333
Size: 530 1006 6166
Fragments arranged by size:
6166 1006 530
NheI G'CTAG_C
Cuts at: 1052 3976 1052
Size: 2924 4778
NotI GC'GGCC_GC
Cuts at: 5149 5149
Size: 7702
NspI r_CATG'y
Cuts at: 1132 1229 2176 2497 4926 5060 6605 1132
Size: 97 947 321 2429 134 1545 2229
Fragments arranged by size:
2429 2229 1545 947 321 134 97
PasI CC'CwG_GG
Cuts at: 1348 3217 3655 4313 1348
Size: 1869 438 658 4737
Fragments arranged by size:
4737 1869 658 438
PciI A'CATG_T
Cuts at: 6601 6601
Size: 7702
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 4819 1108
Size: 902 2809 3991
Fragments arranged by size:
3991 2809 902
PfoI T'CCnGG_A
Cuts at: 3765 4098 4515 3765
Size: 333 417 6952
Fragments arranged by size:
6952 417 333
PmeI GTTT'AAAC
Cuts at: 5006 5006
Size: 7702
PmlI CAC'GTG
Cuts at: 3745 4177 4255 4597 3745
Size: 432 78 342 6850
Fragments arranged by size:
6850 432 342 78
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 5771 5803 7284 7316 3362
Size: 32 2377 32 1481 32 3748
Fragments arranged by size:
3748 2377 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2115 3886 4309 4569 2115
Size: 1771 423 260 5248
Fragments arranged by size:
5248 1771 423 260
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7702
PsiI TTA'TAA
Cuts at: 5272 5272
Size: 7702
PspOMI G'GGCC_C
Cuts at: 3408 3780 4316 4549 4613 3408
Size: 372 536 233 64 6497
Fragments arranged by size:
6497 536 372 233 64
PstI C_TGCA'G
Cuts at: 830 2067 2877 3177 4143 4266 4833 5054 830
Size: 1237 810 300 966 123 567 221 3478
Fragments arranged by size:
3478 1237 966 810 567 300 221 123
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7702
PvuII CAG'CTG
Cuts at: 4012 4276 4327 4816 4012
Size: 264 51 489 6898
Fragments arranged by size:
6898 489 264 51
RsrII CG'GwC_CG
Cuts at: 3037 3037
Size: 7702
SacI G_AGCT'C
Cuts at: 2514 2514
Size: 7702
SacII CC_GC'GG
Cuts at: 3043 4444 3043
Size: 1401 6301
SalI G'TCGA_C
Cuts at: 1936 5044 1936
Size: 3108 4594
SanDI GG'GwC_CC
Cuts at: 4309 4569 4309
Size: 260 7442
SbfI CC_TGCA'GG
Cuts at: 5054 5054
Size: 7702
ScaI AGT'ACT
Cuts at: 5950 7662 5950
Size: 1712 5990
SfcI C'TryA_G
Cuts at: 826 1045 2063 2873 3173 4139 4262 4340 4829
Size: 219 1018 810 300 966 123 78 489
Cuts at: 4829 5050 6185 6866 7057 826
Size: 221 1135 681 191 1471
Fragments arranged by size:
1471 1135 1018 966 810 681 489 300
221 219 191 123 78
SfoI GGC'GCC
Cuts at: 3500 4417 4603 3500
Size: 917 186 6599
Fragments arranged by size:
6599 917 186
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7702
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7702
SmaI CCC'GGG
Cuts at: 1872 3094 3365 3435 3476 4548 5029 1872
Size: 1222 271 70 41 1072 481 4545
Fragments arranged by size:
4545 1222 1072 481 271 70 41
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 2798 3962 4484 5806 6707
Size: 197 926 135 720 1164 522 1322 901
Cuts at: 6707 6969 820
Size: 262 1553
Fragments arranged by size:
1553 1322 1164 926 901 720 522 262
197 135
SnaBI TAC'GTA
Cuts at: 493 5493 493
Size: 5000 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7702
SphI G_CATG'C
Cuts at: 1132 2176 4926 5060 1132
Size: 1044 2750 134 3774
Fragments arranged by size:
3774 2750 1044 134
SrfI GCCC'GGGC
Cuts at: 3476 3476
Size: 7702
SspI AAT'ATT
Cuts at: 5 52 5626 5
Size: 47 5574 2081
Fragments arranged by size:
5574 2081 47
StuI AGG'CCT
Cuts at: 1517 4346 1517
Size: 2829 4873
StyI C'CwwG_G
Cuts at: 513 1065 2004 2849 3526 5141 513
Size: 552 939 845 677 1615 3074
Fragments arranged by size:
3074 1615 939 845 677 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3112 3687 3740 4197 5729 5746 5899
Size: 1255 1532 575 53 457 1532 17 153
Cuts at: 5899 6084 6529 325
Size: 185 445 1498
Fragments arranged by size:
1532 1532 1498 1255 575 457 445 185
153 53 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5948 7660 96
Size: 274 80 33 51 1443 3971 1712 138
Fragments arranged by size:
3971 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2186 2218 2440 2472 2551 2583 2607 2639 2786
Size: 32 222 32 79 32 24 32 147
Cuts at: 2786 2818 3250 3282 3429 3461 3941 3973 4193
Size: 32 432 32 147 32 480 32 220
Cuts at: 4193 4225 7662 7694 2186
Size: 32 3437 32 2194
Fragments arranged by size:
3437 2194 480 432 222 220 147 147
79 32 32 32 32 32 32 32
32 32 32 24
Tth111I GACn'n_nGTC
Cuts at: 1316 2921 3134 1316
Size: 1605 213 5884
Fragments arranged by size:
5884 1605 213
XbaI T'CTAG_A
Cuts at: 5038 5038
Size: 7702
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3346 3604 3346
Size: 258 7444
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7702
XmaI C'CCGG_G
Cuts at: 1870 3092 3363 3433 3474 4546 5027 1870
Size: 1222 271 70 41 1072 481 4545
Fragments arranged by size:
4545 1222 1072 481 271 70 41
XmnI GAAnn'nnTTC
Cuts at: 5831 5831
Size: 7702
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3805 5510 276
Size: 53 83 186 936 2271 1705 2468
Fragments arranged by size:
2468 2271 1705 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI
NsiI PacI PspXI PsrI SapI SexAI SfiI SwaI