Restriction map for
pFN21AE3567
(Circular) (Six-base) MAPSORT of: pFN21AE3567.seq Check: 8579 from: 1 to: 8026
pFN21AE3567 8026 bp
With 182 enzymes: SgfI *
March 28, 2012 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2544 2544
Size: 8026
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5836 278
Size: 53 83 186 936 4300 2468
Fragments arranged by size:
4300 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2133 5369 1937
Size: 196 3236 4594
Fragments arranged by size:
4594 3236 196
Acc65I G'GTAC_C
Cuts at: 5347 5347
Size: 8026
AclI AA'CG_TT
Cuts at: 1563 2699 2730 4695 6153 6526 1563
Size: 1136 31 1965 1458 373 3063
Fragments arranged by size:
3063 1965 1458 1136 373 31
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2199 3188 5152 5176 6089 7473 7741 1632
Size: 567 989 1964 24 913 1384 268 1917
Fragments arranged by size:
1964 1917 1384 989 913 567 268 24
AflII C'TTAA_G
Cuts at: 820 1017 4255 820
Size: 197 3238 4591
Fragments arranged by size:
4591 3238 197
AflIII A'CryG_T
Cuts at: 1203 6925 7902 1203
Size: 5722 977 1327
Fragments arranged by size:
5722 1327 977
AgeI A'CCGG_T
Cuts at: 6857 6857
Size: 8026
AhdI GACnn_n'nnGTC
Cuts at: 2755 6755 2755
Size: 4000 4026
AleI CACnn'nnGTG
Cuts at: 1166 2972 3305 4745 1166
Size: 1806 333 1440 4447
Fragments arranged by size:
4447 1806 1440 333
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4959 1184
Size: 3775 4251
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4551 4583 4551
Size: 32 7994
AlwNI CAG_nnn'CTG
Cuts at: 4687 5769 7341 4687
Size: 1082 1572 5372
Fragments arranged by size:
5372 1572 1082
ApaI G_GGCC'C
Cuts at: 3248 3248
Size: 8026
ApaLI G'TGCA_C
Cuts at: 4791 6083 7239 4791
Size: 1292 1156 5578
Fragments arranged by size:
5578 1292 1156
ApoI r'AATT_y
Cuts at: 1493 2279 2636 2827 3983 4321 4527 5335 5561
Size: 786 357 191 1156 338 206 808 226
Cuts at: 5561 5713 7822 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1156 808 786 357 338 226
206 191 152
AseI AT'TA_AT
Cuts at: 160 1032 6580 160
Size: 872 5548 1606
Fragments arranged by size:
5548 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8026
AvaI C'yCGr_G
Cuts at: 1870 1943 3781 4276 5351 1870
Size: 73 1838 495 1075 4545
Fragments arranged by size:
4545 1838 1075 495 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2400 2433 2400
Size: 33 7993
BamHI G'GATC_C
Cuts at: 4888 5356 5723 4888
Size: 468 367 7191
Fragments arranged by size:
7191 468 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2328 2532 3377 4285
Size: 325 217 627 242 299 204 845 908
Cuts at: 4285 5347 6802 618
Size: 1062 1455 1842
Fragments arranged by size:
1842 1455 1062 908 845 627 325 299
242 217 204
BanII G_rGCy'C
Cuts at: 1391 3248 3788 4092 4524 4930 5345 1391
Size: 1857 540 304 432 406 415 4072
Fragments arranged by size:
4072 1857 540 432 415 406 304
BbeI G_GCGC'C
Cuts at: 2033 2033
Size: 8026
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2451 3159 3999 928
Size: 971 552 708 840 4955
Fragments arranged by size:
4955 971 840 708 552
BbvCI CC'TCA_GC
Cuts at: 4707 4707
Size: 8026
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6217 6251 1903
Size: 34 50 34 4196 34 3678
Fragments arranged by size:
4196 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5918 7128 5918
Size: 1210 6816
BclI T'GATC_A
Cuts at: 1548 3876 1548
Size: 2328 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2111 6637 136
Size: 107 122 71 631 605 439 4526 1525
Fragments arranged by size:
4526 1525 631 605 439 122 107 71
BglII A'GATC_T
Cuts at: 3348 3639 8021 3348
Size: 291 4382 3353
Fragments arranged by size:
4382 3353 291
BlpI GC'TnA_GC
Cuts at: 3290 5443 3290
Size: 2153 5873
Bme1580I G_kGCm'C
Cuts at: 1790 2535 3248 4290 4795 6087 7243 1790
Size: 745 713 1042 505 1292 1156 2573
Fragments arranged by size:
2573 1292 1156 1042 745 713 505
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8026
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5757 6715 442
Size: 416 53 83 399 24 4340 958 1753
Fragments arranged by size:
4340 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8026
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4195 4195
Size: 8026
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2412 3421 3905 4297 4741 4807 4944
Size: 1046 127 1009 484 392 444 66 137
Cuts at: 4944 6686 1239
Size: 1742 2579
Fragments arranged by size:
2579 1742 1046 1009 484 444 392 137
127 66
Bpu10I CC'TnA_GC
Cuts at: 2115 4707 2115
Size: 2592 5434
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3497 6151 7016 7314 3497
Size: 2654 865 298 4209
Fragments arranged by size:
4209 2654 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2322 3693 4081 4583 6689 882
Size: 617 823 1371 388 502 2106 2219
Fragments arranged by size:
2219 2106 1371 823 617 502 388
BsaAI yAC'GTr
Cuts at: 493 1204 5817 493
Size: 711 4613 2702
Fragments arranged by size:
4613 2702 711
BsaBI GATnn'nnATC
Cuts at: 8020 8020
Size: 8026
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 5066 5833 6215
Size: 53 83 186 936 497 3036 767 382
Cuts at: 6215 6850 275
Size: 635 1451
Fragments arranged by size:
3036 1451 936 767 635 497 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2602 6459 6857 7131 7278 7689 2602
Size: 3857 398 274 147 411 2939
Fragments arranged by size:
3857 2939 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2358 2388 2488 2518 4551
Size: 30 165 30 955 30 100 30 2033
Cuts at: 4551 4581 1178
Size: 30 4623
Fragments arranged by size:
4623 2033 955 165 100 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 4163 4166 4169 4184 4524 4527 4601
Size: 1096 1881 3 3 15 340 3 74
Cuts at: 4601 4885 5101 5171 1186
Size: 284 216 70 4041
Fragments arranged by size:
4041 1881 1096 340 284 216 74 70
15 3 3 3
BseYI C'CCAG_C
Cuts at: 2673 7229 7725 2673
Size: 4556 496 2974
Fragments arranged by size:
4556 2974 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3346 4180 1163
Size: 573 1610 834 5009
Fragments arranged by size:
5009 1610 834 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5476 6237 7265 1540
Size: 275 186 3475 761 1028 2301
Fragments arranged by size:
3475 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2774 3724 4795 4930 6087 6172 7243 2774
Size: 950 1071 135 1157 85 1071 3557
Fragments arranged by size:
3557 1157 1071 1071 950 135 85
BsmI GAATG_Cn'
Cuts at: 5537 5630 5537
Size: 93 7933
BsmBI CGTCTCn'nnnn_
Cuts at: 2491 3328 2491
Size: 837 7189
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2535 2774 3248 3724 3788 4092 4290
Size: 399 745 239 474 476 64 304 198
Cuts at: 4290 4524 4795 4930 5345 6087 6172 7243 1391
Size: 234 271 135 415 742 85 1071 2174
Fragments arranged by size:
2174 1071 745 742 476 474 415 399
304 271 239 234 198 135 85 64
BspEI T'CCGG_A
Cuts at: 2602 7689 2602
Size: 5087 2939
BspHI T'CATG_A
Cuts at: 3190 4033 4930 5915 7645 3190
Size: 843 897 985 1730 3571
Fragments arranged by size:
3571 1730 985 897 843
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2544 5381 844
Size: 1700 2837 3489
Fragments arranged by size:
3489 2837 1700
BsrBI CCG'CTC
Cuts at: 1394 1823 3275 5913 1394
Size: 429 1452 2638 3507
Fragments arranged by size:
3507 2638 1452 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2752 2787 6521 6695 65
Size: 1183 1504 35 3734 174 1396
Fragments arranged by size:
3734 1504 1396 1183 174 35
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 6670 6857 1700
Size: 110 198 4662 187 2869
Fragments arranged by size:
4662 2869 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8026
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8026
BssSI C'ACGA_G
Cuts at: 2981 6086 7098 2981
Size: 3105 1012 3909
Fragments arranged by size:
3909 3105 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2242 3101 3523 794
Size: 1448 859 422 5297
Fragments arranged by size:
5297 1448 859 422
BstBI TT'CG_AA
Cuts at: 6830 6830
Size: 8026
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8026
BstXI CCAn_nnnn'nTGG
Cuts at: 4922 5217 4922
Size: 295 7731
BstYI r'GATC_y
Cuts at: 1972 3348 3469 3639 4205 4546 4888 5213 5356
Size: 1376 121 170 566 341 342 325 143
Cuts at: 5356 5723 6108 6125 7577 7663 7675 8021 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1376 566 385 367 346 342
341 325 170 143 121 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3683 3683
Size: 8026
BtgI C'CryG_G
Cuts at: 513 1065 3789 513
Size: 552 2724 4750
Fragments arranged by size:
4750 2724 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2041 487
Size: 683 56 815 6472
Fragments arranged by size:
6472 815 683 56
BtsI GCAGTG_nn'
Cuts at: 2799 4477 5542 6336 6356 2799
Size: 1678 1065 794 20 4469
Fragments arranged by size:
4469 1678 1065 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8026
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2050 2085 529
Size: 35 1486 35 6470
Fragments arranged by size:
6470 1486 35 35
DraI TTT'AAA
Cuts at: 2665 2826 5330 2665
Size: 161 2504 5361
Fragments arranged by size:
5361 2504 161
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7033 809
Size: 507 5717 1802
Fragments arranged by size:
5717 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2857 5473 6362
Size: 54 1425 50 275 18 1027 2616 889
Cuts at: 6362 7991 8
Size: 1629 43
Fragments arranged by size:
2616 1629 1425 1027 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5473 1537
Size: 275 3661 4090
Fragments arranged by size:
4090 3661 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 2539 4140 4161 4577 5119 5956 1353
Size: 833 353 1601 21 416 542 837 3423
Fragments arranged by size:
3423 1601 837 833 542 416 353 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6613 6987 7133 387
Size: 1064 138 5024 374 146 1280
Fragments arranged by size:
5024 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4928 4928
Size: 8026
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2285 2412 3188 3421 3905 4297
Size: 393 567 86 127 776 233 484 392
Cuts at: 4297 4741 4807 4944 5152 5176 6089 6686 7473
Size: 444 66 137 208 24 913 597 787
Cuts at: 7473 7741 1239
Size: 268 1524
Fragments arranged by size:
1524 913 787 776 597 567 484 444
393 392 268 233 208 137 127 86
66 24
EcoO109I rG'GnC_Cy
Cuts at: 2475 3244 2475
Size: 769 7257
EcoRI G'AATT_C
Cuts at: 2279 5335 5713 2279
Size: 3056 378 4592
Fragments arranged by size:
4592 3056 378
EcoRV GAT'ATC
Cuts at: 4050 4050
Size: 8026
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3658 4224 4503 4865 3658
Size: 566 279 362 6819
Fragments arranged by size:
6819 566 362 279
FspI TGC'GCA
Cuts at: 6532 6532
Size: 8026
HaeII r_GCGC'y
Cuts at: 1678 2033 7173 1678
Size: 355 5140 2531
Fragments arranged by size:
5140 2531 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2488 2520 2741 2773 3432
Size: 32 135 32 916 32 221 32 659
Cuts at: 3432 3464 4034 4066 4484 4516 4873 4880 4905
Size: 32 570 32 418 32 357 7 25
Cuts at: 4905 4912 6667 6699 6741 6773 1373
Size: 7 1755 32 42 32 2626
Fragments arranged by size:
2626 1755 916 659 570 418 357 221
135 42 32 32 32 32 32 32
32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3556 5064 5370 5616 669
Size: 988 281 1618 1508 306 246 3079
Fragments arranged by size:
3079 1618 1508 988 306 281 246
HindIII A'AGCT_T
Cuts at: 748 4258 748
Size: 3510 4516
HpaI GTT'AAC
Cuts at: 5616 5616
Size: 8026
KasI G'GCGC_C
Cuts at: 2029 2029
Size: 8026
KpnI G_GTAC'C
Cuts at: 5351 5351
Size: 8026
MfeI C'AATT_G
Cuts at: 5625 5625
Size: 8026
MluI A'CGCG_T
Cuts at: 7902 7902
Size: 8026
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4210 7140 7324 1305
Size: 2905 2930 184 2007
Fragments arranged by size:
2930 2905 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2859 10
Size: 54 1425 343 1027 5177
Fragments arranged by size:
5177 1425 1027 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2326 2792 2972 3086 3305 3620 4038
Size: 648 1160 466 180 114 219 315 418
Cuts at: 4038 4745 5984 6343 6502 518
Size: 707 1239 359 159 2042
Fragments arranged by size:
2042 1239 1160 707 648 466 418 359
315 219 180 159 114
MspA1I CmG'CkG
Cuts at: 1606 5442 6119 7267 7512 1606
Size: 3836 677 1148 245 2120
Fragments arranged by size:
3836 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2030 2030
Size: 8026
NcoI C'CATG_G
Cuts at: 513 1065 3789 513
Size: 552 2724 4750
Fragments arranged by size:
4750 2724 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8026
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8026
NotI GC'GGCC_GC
Cuts at: 5473 5473
Size: 8026
NspI r_CATG'y
Cuts at: 1132 1229 5384 6929 1132
Size: 97 4155 1545 2229
Fragments arranged by size:
4155 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8026
PciI A'CATG_T
Cuts at: 6925 6925
Size: 8026
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3224 4556 4687 1108
Size: 2116 1332 131 4447
Fragments arranged by size:
4447 2116 1332 131
PfoI T'CCnGG_A
Cuts at: 2046 2046
Size: 8026
PmeI GTTT'AAAC
Cuts at: 5330 5330
Size: 8026
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4551 4583 4912 4944 6095 6127 7608 7640 4551
Size: 32 329 32 1151 32 1481 32 4937
Fragments arranged by size:
4937 1481 1151 329 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2475 2475
Size: 8026
PshAI GACnn'nnGTC
Cuts at: 1613 4431 1613
Size: 2818 5208
PsiI TTA'TAA
Cuts at: 5596 5596
Size: 8026
PspOMI G'GGCC_C
Cuts at: 3244 3244
Size: 8026
PstI C_TGCA'G
Cuts at: 830 3033 4948 5076 5378 830
Size: 2203 1915 128 302 3478
Fragments arranged by size:
3478 2203 1915 302 128
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8026
SacI G_AGCT'C
Cuts at: 4930 4930
Size: 8026
SalI G'TCGA_C
Cuts at: 1936 5368 1936
Size: 3432 4594
SanDI GG'GwC_CC
Cuts at: 2475 2475
Size: 8026
SbfI CC_TGCA'GG
Cuts at: 5378 5378
Size: 8026
ScaI AGT'ACT
Cuts at: 2376 3061 6274 7986 2376
Size: 685 3213 1712 2416
Fragments arranged by size:
3213 2416 1712 685
SexAI A'CCwGG_T
Cuts at: 2906 3545 4998 2906
Size: 639 1453 5934
Fragments arranged by size:
5934 1453 639
SfcI C'TryA_G
Cuts at: 826 1045 3029 4944 5072 5374 6509 7190 7381
Size: 219 1984 1915 128 302 1135 681 191
Cuts at: 7381 826
Size: 1471
Fragments arranged by size:
1984 1915 1471 1135 681 302 219 191
128
SfoI GGC'GCC
Cuts at: 2031 2031
Size: 8026
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8026
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8026
SmaI CCC'GGG
Cuts at: 1872 5353 1872
Size: 3481 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3512 4255 6130 7031 7293 820
Size: 197 926 1569 743 1875 901 262 1553
Fragments arranged by size:
1875 1569 1553 926 901 743 262 197
SnaBI TAC'GTA
Cuts at: 493 5817 493
Size: 5324 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8026
SphI G_CATG'C
Cuts at: 1132 5384 1132
Size: 4252 3774
SspI AAT'ATT
Cuts at: 5 52 3533 5950 5
Size: 47 3481 2417 2081
Fragments arranged by size:
3481 2417 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8026
StyI C'CwwG_G
Cuts at: 513 1065 3237 3393 3789 4092 4436 5465 513
Size: 552 2172 156 396 303 344 1029 3074
Fragments arranged by size:
3074 2172 1029 552 396 344 303 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6053 6070 6223 6408 6853 325
Size: 1255 4473 17 153 185 445 1498
Fragments arranged by size:
4473 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2374 3059 3279
Size: 274 80 33 51 1443 397 685 220
Cuts at: 3279 3432 6272 7984 96
Size: 153 2840 1712 138
Fragments arranged by size:
2840 1712 1443 685 397 274 220 153
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7986 8018 7986
Size: 32 7994
Tth111I GACn'n_nGTC
Cuts at: 1316 2450 2502 1316
Size: 1134 52 6840
Fragments arranged by size:
6840 1134 52
XbaI T'CTAG_A
Cuts at: 5362 5362
Size: 8026
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2107 2107
Size: 8026
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8026
XmaI C'CCGG_G
Cuts at: 1870 5351 1870
Size: 3481 4545
XmnI GAAnn'nnTTC
Cuts at: 3479 3534 3568 3996 4695 6155 3479
Size: 55 34 428 699 1460 5350
Fragments arranged by size:
5350 1460 699 428 55 34
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5834 276
Size: 53 83 186 936 4300 2468
Fragments arranged by size:
4300 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BsiWI BstZ17I DraIII EcoNI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacII SapI SfiI SrfI
SwaI