Restriction map for
pFN21AE3562
(Circular) (Six-base) MAPSORT of: pFN21AE3562.seq Check: 8696 from: 1 to: 6055
pFN21AE3562 6055 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2054 2054
Size: 6055
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2674 2984 3398 1937
Size: 737 310 414 4594
Fragments arranged by size:
4594 737 414 310
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2723 2842 4118 5502 5770 1632
Size: 1091 119 1276 1384 268 1917
Fragments arranged by size:
1917 1384 1276 1091 268 119
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5858
AflIII A'CryG_T
Cuts at: 1203 2955 4954 5931 1203
Size: 1752 1999 977 1327
Fragments arranged by size:
1999 1752 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 4784 4784
Size: 6055
AleI CACnn'nnGTG
Cuts at: 1166 2834 1166
Size: 1668 4387
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6055
AlwNI CAG_nnn'CTG
Cuts at: 2078 3798 5370 2078
Size: 1720 1572 2763
Fragments arranged by size:
2763 1720 1572
ApaLI G'TGCA_C
Cuts at: 4112 5268 4112
Size: 1156 4899
ApoI r'AATT_y
Cuts at: 1493 2521 3364 3590 3742 5851 1493
Size: 1028 843 226 152 2109 1697
Fragments arranged by size:
2109 1697 1028 843 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4609 160
Size: 872 3577 1606
Fragments arranged by size:
3577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 3380 1870
Size: 73 1437 4545
Fragments arranged by size:
4545 1437 73
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3376 4831 618
Size: 325 217 627 1589 1455 1842
Fragments arranged by size:
1842 1589 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2043 3004 3374 1391
Size: 652 961 370 4072
Fragments arranged by size:
4072 961 652 370
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2922 928
Size: 971 1023 4061
Fragments arranged by size:
4061 1023 971
BbvCI CC'TCA_GC
Cuts at: 2793 3011 2793
Size: 218 5837
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4246 4280 1903
Size: 34 2309 34 3678
Fragments arranged by size:
3678 2309 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 2538 1548
Size: 990 5065
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4666 136
Size: 107 122 71 631 605 2994 1525
Fragments arranged by size:
2994 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6050 6050
Size: 6055
BlpI GC'TnA_GC
Cuts at: 3472 3472
Size: 6055
Bme1580I G_kGCm'C
Cuts at: 1790 2100 4116 5272 1790
Size: 310 2016 1156 2573
Fragments arranged by size:
2573 2016 1156 310
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2841 3040 3786
Size: 416 53 83 399 24 1424 199 746
Cuts at: 3786 4744 442
Size: 958 1753
Fragments arranged by size:
1753 1424 958 746 416 399 199 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4715 1239
Size: 3476 2579
Bpu10I CC'TnA_GC
Cuts at: 2793 3011 2793
Size: 218 5837
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2624 4180 5045 5343 2624
Size: 1556 865 298 3336
Fragments arranged by size:
3336 1556 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2480 4718 882
Size: 617 981 2238 2219
Fragments arranged by size:
2238 2219 981 617
BsaAI yAC'GTr
Cuts at: 493 1204 2596 3846 493
Size: 711 1392 1250 2702
Fragments arranged by size:
2702 1392 1250 711
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3862 4244 4879 275
Size: 53 83 186 936 2329 382 635 1451
Fragments arranged by size:
2329 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4488 4886 5160 5307 5718 4488
Size: 398 274 147 411 4825
Fragments arranged by size:
4825 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2131 2161 1178
Size: 30 165 30 728 30 5072
Fragments arranged by size:
5072 728 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6055
BseYI C'CCAG_C
Cuts at: 5258 5754 5258
Size: 496 5559
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2041 2188 2956 1163
Size: 573 305 147 768 4262
Fragments arranged by size:
4262 768 573 305 147
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3505 4266 5294 1540
Size: 275 186 1504 761 1028 2301
Fragments arranged by size:
2301 1504 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 3004 4116 4201 5272 2043
Size: 961 1112 85 1071 2826
Fragments arranged by size:
2826 1112 1071 961 85
BsmI GAATG_Cn'
Cuts at: 3566 3659 3566
Size: 93 5962
BsmBI CGTCTCn'nnnn_
Cuts at: 2745 2745
Size: 6055
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2100 3004 3374 4116 4201 5272
Size: 399 253 57 904 370 742 85 1071
Cuts at: 5272 1391
Size: 2174
Fragments arranged by size:
2174 1071 904 742 399 370 253 85
57
BspEI T'CCGG_A
Cuts at: 5718 5718
Size: 6055
BspHI T'CATG_A
Cuts at: 3944 5674 3944
Size: 1730 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2054 2742 3410 844
Size: 1210 688 668 3489
Fragments arranged by size:
3489 1210 688 668
BsrBI CCG'CTC
Cuts at: 1394 1823 3942 1394
Size: 429 2119 3507
Fragments arranged by size:
3507 2119 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4550 4724 65
Size: 1183 3302 174 1396
Fragments arranged by size:
3302 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4699 4886 1700
Size: 110 2889 187 2869
Fragments arranged by size:
2889 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 4115 5127 4115
Size: 1012 5043
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6055
BstBI TT'CG_AA
Cuts at: 2913 4859 2913
Size: 1946 4109
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6055
BstYI r'GATC_y
Cuts at: 1972 3006 3347 3385 3752 4137 4154 5606 5692
Size: 1034 341 38 367 385 17 1452 86
Cuts at: 5692 5704 6050 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1034 385 367 346 341 86
38 17 12
BstZ17I GTA'TAC
Cuts at: 2675 2985 2675
Size: 310 5745
Bsu36I CC'TnA_GG
Cuts at: 2556 2739 2921 2556
Size: 183 182 5690
Fragments arranged by size:
5690 183 182
BtgI C'CryG_G
Cuts at: 513 1065 2004 3343 513
Size: 552 939 1339 3225
Fragments arranged by size:
3225 1339 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5316
Fragments arranged by size:
5316 683 56
BtsI GCAGTG_nn'
Cuts at: 2030 2939 3571 4365 4385 2030
Size: 909 632 794 20 3700
Fragments arranged by size:
3700 909 794 632 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DraIII CAC_nnn'GTG
Cuts at: 2025 2025
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2784 3502 4391
Size: 54 1425 50 275 18 954 718 889
Cuts at: 4391 6020 8
Size: 1629 43
Fragments arranged by size:
1629 1425 954 889 718 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3502 1537
Size: 275 1690 4090
Fragments arranged by size:
4090 1690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2577 3176 3985 1353
Size: 1224 599 809 3423
Fragments arranged by size:
3423 1224 809 599
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4642 5016 5162 387
Size: 1064 138 3053 374 146 1280
Fragments arranged by size:
3053 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2041 3002 2041
Size: 961 5094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2723 2842 4118 4715 5502 5770 1239
Size: 393 1091 119 1276 597 787 268 1524
Fragments arranged by size:
1524 1276 1091 787 597 393 268 119
EcoNI CCTnn'n_nnAGG
Cuts at: 2738 2738
Size: 6055
EcoO109I rG'GnC_Cy
Cuts at: 3210 3210
Size: 6055
EcoRI G'AATT_C
Cuts at: 3364 3742 3364
Size: 378 5677
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 5202 1678
Size: 3524 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2229 2261 2853 2885 2991
Size: 32 135 32 657 32 592 32 106
Cuts at: 2991 3023 4696 4728 4770 4802 1373
Size: 32 1673 32 42 32 2626
Fragments arranged by size:
2626 1673 657 592 135 106 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3399 3645 669
Size: 988 281 1461 246 3079
Fragments arranged by size:
3079 1461 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2903 748
Size: 2155 3900
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2993 5169 5353 1305
Size: 1688 2176 184 2007
Fragments arranged by size:
2176 2007 1688 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2786 10
Size: 54 1425 343 954 3279
Fragments arranged by size:
3279 1425 954 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2834 4013 4372 4531 518
Size: 648 1668 1179 359 159 2042
Fragments arranged by size:
2042 1668 1179 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3471 4148 5296 5541 1606
Size: 1865 677 1148 245 2120
Fragments arranged by size:
2120 1865 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4564
Fragments arranged by size:
4564 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 2369 2959 3109 3413 4958 1132
Size: 97 1140 590 150 304 1545 2229
Fragments arranged by size:
2229 1545 1140 590 304 150 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6055
PciI A'CATG_T
Cuts at: 2955 4954 2955
Size: 1999 4056
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6055
PfoI T'CCnGG_A
Cuts at: 3234 3234
Size: 6055
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6055
PsiI TTA'TAA
Cuts at: 2519 3625 2519
Size: 1106 4949
PstI C_TGCA'G
Cuts at: 830 3407 830
Size: 2577 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
SacI G_AGCT'C
Cuts at: 2043 3004 2043
Size: 961 5094
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SapI GCTCTTCn'nnn_
Cuts at: 2577 2577
Size: 6055
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SfcI C'TryA_G
Cuts at: 826 1045 2281 2410 3403 4538 5219 5410 826
Size: 219 1236 129 993 1135 681 191 1471
Fragments arranged by size:
1471 1236 1135 993 681 219 191 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 3382 1872
Size: 1510 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2639 4159 5060 5322 820
Size: 197 926 696 1520 901 262 1553
Fragments arranged by size:
1553 1520 926 901 696 262 197
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 2369 3413 1132
Size: 1237 1044 3774
Fragments arranged by size:
3774 1237 1044
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6055
StyI C'CwwG_G
Cuts at: 513 1065 2004 2360 2874 3206 3214 3494 513
Size: 552 939 356 514 332 8 280 3074
Fragments arranged by size:
3074 939 552 514 356 332 280 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4082 4099 4252 4437 4882 325
Size: 1255 2502 17 153 185 445 1498
Fragments arranged by size:
2502 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2190 2975 4301
Size: 274 80 33 51 1443 213 785 1326
Cuts at: 4301 6013 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1326 785 274 213 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6015 6047 6015
Size: 32 6023
Tth111I GACn'n_nGTC
Cuts at: 1316 2078 1316
Size: 762 5293
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 3380 1870
Size: 1510 4545
XmnI GAAnn'nnTTC
Cuts at: 4184 4184
Size: 6055
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI