Restriction map for
pFN21AE3555
(Circular) (Six-base) MAPSORT of: pFN21AE3555.seq Check: 6064 from: 1 to: 20326
pFN21AE3555 20326 bp
With 182 enzymes: SgfI *
April 17, 2013 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6057 7976 8405 11922 16197 6057
Size: 1919 429 3517 4275 10186
Fragments arranged by size:
10186 4275 3517 1919 429
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8318 9039 11472 11655
Size: 53 83 186 936 6782 721 2433 183
Cuts at: 11655 18136 278
Size: 6481 2468
Fragments arranged by size:
6782 6481 2468 2433 936 721 186 183
83 53
AccI GT'mk_AC
Cuts at: 1937 7545 7677 9093 9699 14214 16689 17150 17669
Size: 5608 132 1416 606 4515 2475 461 519
Cuts at: 17669 1937
Size: 4594
Fragments arranged by size:
5608 4594 4515 2475 1416 606 519 461
132
Acc65I G'GTAC_C
Cuts at: 3799 7266 9323 14982 17647 3799
Size: 3467 2057 5659 2665 6478
Fragments arranged by size:
6478 5659 3467 2665 2057
AclI AA'CG_TT
Cuts at: 1563 4452 18453 18826 1563
Size: 2889 14001 373 3063
Fragments arranged by size:
14001 3063 2889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 2727 2997 3033 3601 4211 4764 5030
Size: 557 538 270 36 568 610 553 266
Cuts at: 5030 5170 5507 6103 6347 6442 6581 7816 8322
Size: 140 337 596 244 95 139 1235 506
Cuts at: 8322 10307 10421 10595 10948 11888 13280 13649 14517
Size: 1985 114 174 353 940 1392 369 868
Cuts at: 14517 15095 15477 15887 18389 19773 20041 1632
Size: 578 382 410 2502 1384 268 1917
Fragments arranged by size:
2502 1985 1917 1392 1384 1235 940 868
610 596 578 568 557 553 538 506
410 382 369 353 337 270 268 266
244 174 140 139 114 95 36
AfeI AGC'GCT
Cuts at: 8343 9932 17456 8343
Size: 1589 7524 11213
Fragments arranged by size:
11213 7524 1589
AflII C'TTAA_G
Cuts at: 820 1017 4589 5966 7228 14311 14618 820
Size: 197 3572 1377 1262 7083 307 6528
Fragments arranged by size:
7083 6528 3572 1377 1262 307 197
AflIII A'CryG_T
Cuts at: 1203 3793 8351 8353 8547 12720 15710 17190 19225
Size: 2590 4558 2 194 4173 2990 1480 2035
Cuts at: 19225 20202 1203
Size: 977 1327
Fragments arranged by size:
4558 4173 2990 2590 2035 1480 1327 977
194 2
AgeI A'CCGG_T
Cuts at: 2666 19157 2666
Size: 16491 3835
AhdI GACnn_n'nnGTC
Cuts at: 4545 14221 19055 4545
Size: 9676 4834 5816
Fragments arranged by size:
9676 5816 4834
AleI CACnn'nnGTG
Cuts at: 1166 3727 6818 11991 12043 16940 1166
Size: 2561 3091 5173 52 4897 4552
Fragments arranged by size:
5173 4897 4552 3091 2561 52
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4149 6272 7048 10008 10956 11127 13615 13625
Size: 2965 2123 776 2960 948 171 2488 10
Cuts at: 13625 1184
Size: 7885
Fragments arranged by size:
7885 2965 2960 2488 2123 948 776 171
10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2102 2134 2102
Size: 32 20294
AlwNI CAG_nnn'CTG
Cuts at: 2105 2167 2355 3056 6265 6517 7570 7924 8459
Size: 62 188 701 3209 252 1053 354 535
Cuts at: 8459 9064 9538 9805 11167 13843 14073 14950 15805
Size: 605 474 267 1362 2676 230 877 855
Cuts at: 15805 17248 17275 17597 18069 19641 2105
Size: 1443 27 322 472 1572 2790
Fragments arranged by size:
3209 2790 2676 1572 1443 1362 1053 877
855 701 605 535 474 472 354 322
267 252 230 188 62 27
ApaI G_GGCC'C
Cuts at: 2186 10370 10669 2186
Size: 8184 299 11843
Fragments arranged by size:
11843 8184 299
ApaLI G'TGCA_C
Cuts at: 5977 7750 9356 13445 14048 18383 19539 5977
Size: 1773 1606 4089 603 4335 1156 6764
Fragments arranged by size:
6764 4335 4089 1773 1606 1156 603
ApoI r'AATT_y
Cuts at: 1493 3224 5075 6142 8199 9296 10850 11997 14129
Size: 1731 1851 1067 2057 1097 1554 1147 2132
Cuts at: 14129 15209 15991 17520 17635 17861 18013 20122 1493
Size: 1080 782 1529 115 226 152 2109 1697
Fragments arranged by size:
2132 2109 2057 1851 1731 1697 1554 1529
1147 1097 1080 1067 782 226 152 115
AseI AT'TA_AT
Cuts at: 160 1032 18880 160
Size: 872 17848 1606
Fragments arranged by size:
17848 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 20326
AvaI C'yCGr_G
Cuts at: 1870 1943 2197 2225 2455 2762 3127 4511 7165
Size: 73 254 28 230 307 365 1384 2654
Cuts at: 7165 7468 7871 8824 9721 11015 11721 12267 13418
Size: 303 403 953 897 1294 706 546 1151
Cuts at: 13418 13829 15041 15621 17651 1870
Size: 411 1212 580 2030 4545
Fragments arranged by size:
4545 2654 2030 1384 1294 1212 1151 953
897 706 580 546 411 403 365 307
303 254 230 73 28
AvrII C'CTAG_G
Cuts at: 5117 13807 5117
Size: 8690 11636
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 8340 8373 15502 15535 8340
Size: 33 7129 33 13131
Fragments arranged by size:
13131 7129 33 33
BamHI G'GATC_C
Cuts at: 9150 9243 14697 15835 17125 17656 18023 9150
Size: 93 5454 1138 1290 531 367 11453
Fragments arranged by size:
11453 5454 1290 1138 531 367 93
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3799 4082 4784 6652 7266
Size: 325 217 627 2012 283 702 1868 614
Cuts at: 7266 7365 7864 8114 8579 9079 9323 11089 14826
Size: 99 499 250 465 500 244 1766 3737
Cuts at: 14826 14982 17647 19102 618
Size: 156 2665 1455 1842
Fragments arranged by size:
3737 2665 2012 1868 1842 1766 1455 702
627 614 500 499 465 325 283 250
244 217 156 99
BanII G_rGCy'C
Cuts at: 1391 2186 2204 2235 2727 2763 3128 3470 5018
Size: 795 18 31 492 36 365 342 1548
Cuts at: 5018 5648 5784 6204 6716 7152 7296 7661 8336
Size: 630 136 420 512 436 144 365 675
Cuts at: 8336 9189 9677 10370 10669 10734 11577 12028 13689
Size: 853 488 693 299 65 843 451 1661
Cuts at: 13689 13759 15396 17336 17645 1391
Size: 70 1637 1940 309 4072
Fragments arranged by size:
4072 1940 1661 1637 1548 853 843 795
693 675 630 512 492 488 451 436
420 365 365 342 309 299 144 136
70 65 36 31 18
BbeI G_GCGC'C
Cuts at: 4086 8118 4086
Size: 4032 16294
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2885 3021 3196 4702 5176 6569 6832
Size: 971 986 136 175 1506 474 1393 263
Cuts at: 6832 7776 7840 10252 10560 10583 10605 10620 11647
Size: 944 64 2412 308 23 22 15 1027
Cuts at: 11647 12456 13852 13871 13907 14564 15465 15730 928
Size: 809 1396 19 36 657 901 265 5524
Fragments arranged by size:
5524 2412 1506 1396 1393 1027 986 971
944 901 809 657 474 308 265 263
175 136 64 36 23 22 19 15
BbvCI CC'TCA_GC
Cuts at: 2803 5561 7337 9543 12181 13970 16337 16442 17354
Size: 2758 1776 2206 2638 1789 2367 105 912
Cuts at: 17354 2803
Size: 5775
Fragments arranged by size:
5775 2758 2638 2367 2206 1789 1776 912
105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2951 2985 4331 4365 7459 7493 14702
Size: 34 1014 34 1346 34 3094 34 7209
Cuts at: 14702 14736 18517 18551 1903
Size: 34 3781 34 3678
Fragments arranged by size:
7209 3781 3678 3094 1346 1014 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3999 5051 12757 14816 18218 19428 3999
Size: 1052 7706 2059 3402 1210 4897
Fragments arranged by size:
7706 4897 3402 2059 1210 1052
BclI T'GATC_A
Cuts at: 1548 5100 9514 9846 11055 17176 1548
Size: 3552 4414 332 1209 6121 4698
Fragments arranged by size:
6121 4698 4414 3552 1209 332
BfrBI ATG'CAT
Cuts at: 4053 4998 10608 4053
Size: 945 5610 13771
Fragments arranged by size:
13771 5610 945
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2436 2813 7538
Size: 107 122 71 631 605 764 377 4725
Cuts at: 7538 7892 13595 14663 15428 16626 18937 136
Size: 354 5703 1068 765 1198 2311 1525
Fragments arranged by size:
5703 4725 2311 1525 1198 1068 765 764
631 605 377 354 122 107 71
BglII A'GATC_T
Cuts at: 8866 10384 11277 12991 20321 8866
Size: 1518 893 1714 7330 8871
Fragments arranged by size:
8871 7330 1714 1518 893
BlpI GC'TnA_GC
Cuts at: 2832 3122 4256 5017 6860 7703 11813 14852 16916
Size: 290 1134 761 1843 843 4110 3039 2064
Cuts at: 16916 17346 17743 2832
Size: 430 397 5415
Fragments arranged by size:
5415 4110 3039 2064 1843 1134 843 761
430 397 290
Bme1580I G_kGCm'C
Cuts at: 1790 2186 2462 5981 7671 7754 8440 9084 9360
Size: 396 276 3519 1690 83 686 644 276
Cuts at: 9360 10189 10370 10669 12860 13449 14052 16302 18387
Size: 829 181 299 2191 589 603 2250 2085
Cuts at: 18387 19543 1790
Size: 1156 2573
Fragments arranged by size:
3519 2573 2250 2191 2085 1690 1156 829
686 644 603 589 396 299 276 276
181 83
BmgBI CAC'GTC
Cuts at: 1318 5182 6912 8176 9925 13858 13897 15703 1318
Size: 3864 1730 1264 1749 3933 39 1806 5941
Fragments arranged by size:
5941 3933 3864 1806 1749 1730 1264 39
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3661 3842 7813
Size: 416 53 83 399 24 2244 181 3971
Cuts at: 7813 8356 9024 10672 13629 13753 15065 15725 16042
Size: 543 668 1648 2957 124 1312 660 317
Cuts at: 16042 16163 18057 19015 442
Size: 121 1894 958 1753
Fragments arranged by size:
3971 2957 2244 1894 1753 1648 1312 958
668 660 543 416 399 317 181 124
121 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 20326
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2097 4195 16457 17502 17504 2097
Size: 2098 12262 1045 2 4919
Fragments arranged by size:
12262 4919 2098 1045 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2498 3509 4159 4190 4840 5342 6209 8582
Size: 1259 1011 650 31 650 502 867 2373
Cuts at: 8582 9056 9373 11531 11564 11951 11953 12827 12830
Size: 474 317 2158 33 387 2 874 3
Cuts at: 12830 13181 13703 14121 14557 14558 14616 15515 15810
Size: 351 522 418 436 1 58 899 295
Cuts at: 15810 15896 16091 16244 16883 18986 1239
Size: 86 195 153 639 2103 2579
Fragments arranged by size:
2579 2373 2158 2103 1259 1011 899 874
867 650 650 639 522 502 474 436
418 387 351 317 295 195 153 86
58 33 31 3 2 1
Bpu10I CC'TnA_GC
Cuts at: 2803 3469 3989 5561 5642 7139 7151 7337 9302
Size: 666 520 1572 81 1497 12 186 1965
Cuts at: 9302 9543 12181 12423 13970 16337 16442 17354 2803
Size: 241 2638 242 1547 2367 105 912 5775
Fragments arranged by size:
5775 2638 2367 1965 1572 1547 1497 912
666 520 242 241 186 105 81 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3005 4430 5831 9146 14573 15456 16553 16907 17139
Size: 1425 1401 3315 5427 883 1097 354 232
Cuts at: 17139 18451 19316 19614 3005
Size: 1312 865 298 3717
Fragments arranged by size:
5427 3717 3315 1425 1401 1312 1097 883
865 354 298 232
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2939 7397 7452 12642 15908 16835 18989
Size: 617 1440 4458 55 5190 3266 927 2154
Cuts at: 18989 882
Size: 2219
Fragments arranged by size:
5190 4458 3266 2219 2154 1440 927 617
55
BsaAI yAC'GTr
Cuts at: 493 1204 3796 8008 8354 10709 11152 11389 13801
Size: 711 2592 4212 346 2355 443 237 2412
Cuts at: 13801 14047 17191 18117 493
Size: 246 3144 926 2702
Fragments arranged by size:
4212 3144 2702 2592 2412 2355 926 711
443 346 246 237
BsaBI GATnn'nnATC
Cuts at: 15812 20320 15812
Size: 4508 15818
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2129 4083 8115 8315
Size: 53 83 186 936 596 1954 4032 200
Cuts at: 8315 9036 10356 11469 11652 11676 13406 17385 18133
Size: 721 1320 1113 183 24 1730 3979 748
Cuts at: 18133 18515 19150 275
Size: 382 635 1451
Fragments arranged by size:
4032 3979 1954 1730 1451 1320 1113 936
748 721 635 596 382 200 186 183
83 53 24
BsaWI w'CCGG_w
Cuts at: 2666 4278 8886 9234 9585 12339 18759 19157 19431
Size: 1612 4608 348 351 2754 6420 398 274
Cuts at: 19431 19578 19989 2666
Size: 147 411 3003
Fragments arranged by size:
6420 4608 3003 2754 1612 411 398 351
348 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2102 2132 2477 2507 2714
Size: 30 165 30 699 30 345 30 207
Cuts at: 2714 2744 2899 2929 3129 3159 4508 4538 5429
Size: 30 155 30 200 30 1349 30 891
Cuts at: 5429 5459 5630 5660 6332 6362 8165 8195 8787
Size: 30 171 30 672 30 1803 30 592
Cuts at: 8787 8817 11837 11867 12024 12054 13154 13184 14759
Size: 30 3020 30 157 30 1100 30 1575
Cuts at: 14759 14789 1178
Size: 30 6715
Fragments arranged by size:
6715 3020 1803 1575 1349 1100 891 699
672 592 345 207 200 171 165 157
155 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 2904 3150 3228 3231 3868 4529 5599
Size: 1057 661 246 78 3 637 661 1070
Cuts at: 5599 6743 6767 7291 7634 7745 8173 8213 8344
Size: 1144 24 524 343 111 428 40 131
Cuts at: 8344 8458 9614 9668 10750 11585 11774 12476 12530
Size: 114 1156 54 1082 835 189 702 54
Cuts at: 12530 12683 13178 13233 13361 14279 16537 16844 17342
Size: 153 495 55 128 918 2258 307 498
Cuts at: 17342 17465 1186
Size: 123 4047
Fragments arranged by size:
4047 2258 1156 1144 1082 1070 1057 918
835 702 661 661 637 524 498 495
428 343 307 246 189 153 131 128
123 114 111 78 55 54 54 40
24 3
BseYI C'CCAG_C
Cuts at: 2418 4078 4876 4947 5666 6291 6446 6890 9670
Size: 1660 798 71 719 625 155 444 2780
Cuts at: 9670 11247 11827 12168 12427 13263 15418 15679 15895
Size: 1577 580 341 259 836 2155 261 216
Cuts at: 15895 17015 19529 20025 2418
Size: 1120 2514 496 2719
Fragments arranged by size:
2780 2719 2514 2155 1660 1577 1120 836
798 719 625 580 496 444 341 261
259 216 155 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3069 3248 4082 6035 6431 6677 6866
Size: 573 1333 179 834 1953 396 246 189
Cuts at: 6866 6872 7195 7582 8210 9922 10450 10568 11900
Size: 6 323 387 628 1712 528 118 1332
Cuts at: 11900 12644 12649 13109 13369 13585 14900 16868 17267
Size: 744 5 460 260 216 1315 1968 399
Cuts at: 17267 17387 1163
Size: 120 4102
Fragments arranged by size:
4102 1968 1953 1712 1333 1332 1315 834
744 628 573 528 460 399 396 387
323 260 246 216 189 179 120 118
6 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9663 14902 17776 18537 19565 1540
Size: 275 186 7662 5239 2874 761 1028 2301
Fragments arranged by size:
7662 5239 2874 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2727 4344 5018 5648 5981 6204 7050 7098 7145
Size: 1617 674 630 333 223 846 48 47
Cuts at: 7145 7152 7296 7754 7812 8163 8336 9189 9360
Size: 7 144 458 58 351 173 853 171
Cuts at: 9360 9966 10734 11030 11577 12693 13449 13689 13707
Size: 606 768 296 547 1116 756 240 18
Cuts at: 13707 13800 13806 13857 13997 14052 17114 18387 18472
Size: 93 6 51 140 55 3062 1273 85
Cuts at: 18472 19543 2727
Size: 1071 3510
Fragments arranged by size:
3510 3062 1617 1273 1116 1071 853 846
768 756 674 630 606 547 458 351
333 296 240 223 173 171 144 140
93 85 58 55 51 48 47 18
7 6
BsmI GAATG_Cn'
Cuts at: 6464 8597 9997 13949 14486 15227 17837 17930 6464
Size: 2133 1400 3952 537 741 2610 93 8860
Fragments arranged by size:
8860 3952 2610 2133 1400 741 537 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2957 9027 9650 10522 12933 15693 16529 2957
Size: 6070 623 872 2411 2760 836 6754
Fragments arranged by size:
6754 6070 2760 2411 872 836 623
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2186 2204 2235 2462 2727 2763 3128
Size: 399 396 18 31 227 265 36 365
Cuts at: 3128 3470 4344 5018 5648 5784 5981 6204 6716
Size: 342 874 674 630 136 197 223 512
Cuts at: 6716 7050 7098 7145 7152 7296 7661 7671 7754
Size: 334 48 47 7 144 365 10 83
Cuts at: 7754 7812 8163 8336 8440 9084 9189 9360 9677
Size: 58 351 173 104 644 105 171 317
Cuts at: 9677 9966 10189 10370 10669 10734 11030 11577 12028
Size: 289 223 181 299 65 296 547 451
Cuts at: 12028 12693 12860 13449 13689 13707 13759 13800 13806
Size: 665 167 589 240 18 52 41 6
Cuts at: 13806 13857 13997 14052 15396 16302 17114 17336 17645
Size: 51 140 55 1344 906 812 222 309
Cuts at: 17645 18387 18472 19543 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1344 1071 906 874 812 742 674
665 644 630 589 547 512 451 399
396 365 365 351 342 334 317 309
299 296 289 265 240 227 223 223
222 197 181 173 171 167 144 140
136 105 104 85 83 65 58 55
52 51 48 47 41 36 31 18
18 10 7 6
BspEI T'CCGG_A
Cuts at: 8886 12339 19989 8886
Size: 3453 7650 9223
Fragments arranged by size:
9223 7650 3453
BspHI T'CATG_A
Cuts at: 3286 3534 6468 8766 9039 9879 10263 14434 18215
Size: 248 2934 2298 273 840 384 4171 3781
Cuts at: 18215 19945 3286
Size: 1730 3667
Fragments arranged by size:
4171 3781 3667 2934 2298 1730 840 384
273 248
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6057 6076 6525 6888 7471 7886 7976 8405
Size: 5213 19 449 363 583 415 90 429
Cuts at: 8405 9318 10396 10512 11325 11689 11922 13242 13965
Size: 913 1078 116 813 364 233 1320 723
Cuts at: 13965 14688 14790 16197 17681 844
Size: 723 102 1407 1484 3489
Fragments arranged by size:
5213 3489 1484 1407 1320 1078 913 813
723 723 583 449 429 415 364 363
233 116 102 90 19
BsrBI CCG'CTC
Cuts at: 1394 1823 4166 14397 16649 16783 18213 1394
Size: 429 2343 10231 2252 134 1430 3507
Fragments arranged by size:
10231 3507 2343 2252 1430 429 134
BsrDI GCAATG_nn'
Cuts at: 65 1248 3803 10938 12174 13599 15197 16210 17229
Size: 1183 2555 7135 1236 1425 1598 1013 1019
Cuts at: 17229 18821 18995 65
Size: 1592 174 1396
Fragments arranged by size:
7135 2555 1598 1592 1425 1396 1236 1183
1019 1013 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2666 4395 7950 11505 15668 18970 19157
Size: 110 856 1729 3555 3555 4163 3302 187
Cuts at: 19157 1700
Size: 2869
Fragments arranged by size:
4163 3555 3555 3302 2869 1729 856 187
110
BsrGI T'GTAC_A
Cuts at: 96 11475 14514 15150 16944 96
Size: 11379 3039 636 1794 3478
Fragments arranged by size:
11379 3478 3039 1794 636
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 20326
BssSI C'ACGA_G
Cuts at: 6725 6735 9959 11132 15269 18386 19398 6725
Size: 10 3224 1173 4137 3117 1012 7653
Fragments arranged by size:
7653 4137 3224 3117 1173 1012 10
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2064 2822 3315 4252 4944 10591 11167 14056
Size: 1270 758 493 937 692 5647 576 2889
Cuts at: 14056 14950 15585 16318 794
Size: 894 635 733 4802
Fragments arranged by size:
5647 4802 2889 1270 937 894 758 733
692 635 576 493
BstBI TT'CG_AA
Cuts at: 19130 19130
Size: 20326
BstEII G'GTnAC_C
Cuts at: 1184 5285 5896 8758 8839 13846 16163 1184
Size: 4101 611 2862 81 5007 2317 5347
Fragments arranged by size:
5347 5007 4101 2862 2317 611 81
BstXI CCAn_nnnn'nTGG
Cuts at: 2426 2476 3280 4001 6550 9346 10240 12355 13271
Size: 50 804 721 2549 2796 894 2115 916
Cuts at: 13271 13681 14005 15895 15925 2426
Size: 410 324 1890 30 6827
Fragments arranged by size:
6827 2796 2549 2115 1890 916 894 804
721 410 324 50 30
BstYI r'GATC_y
Cuts at: 1972 5878 8467 8866 9150 9243 10039 10192 10384
Size: 3906 2589 399 284 93 796 153 192
Cuts at: 10384 11163 11277 12344 12991 14697 15155 15228 15835
Size: 779 114 1067 647 1706 458 73 607
Cuts at: 15835 16381 16585 16605 17125 17326 17656 18023 18408
Size: 546 204 20 520 201 330 367 385
Cuts at: 18408 18425 19877 19963 19975 20321 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
3906 2589 1977 1706 1452 1067 796 779
647 607 546 520 458 399 385 367
346 330 284 204 201 192 153 114
93 86 73 20 17 12
BstZ17I GTA'TAC
Cuts at: 9700 17151 9700
Size: 7451 12875
Bsu36I CC'TnA_GG
Cuts at: 2388 4149 5745 7217 8088 8441 9145 9917 10955
Size: 1761 1596 1472 871 353 704 772 1038
Cuts at: 10955 13512 14066 14742 2388
Size: 2557 554 676 7972
Fragments arranged by size:
7972 2557 1761 1596 1472 1038 871 772
704 676 554 353
BtgI C'CryG_G
Cuts at: 513 1065 2004 2381 4121 4848 5124 5982 6615
Size: 552 939 377 1740 727 276 858 633
Cuts at: 6615 6921 7452 8844 11802 12301 12522 12633 13270
Size: 306 531 1392 2958 499 221 111 637
Cuts at: 13270 13439 15426 15540 16695 16731 513
Size: 169 1987 114 1155 36 4108
Fragments arranged by size:
4108 2958 1987 1740 1392 1155 939 858
727 637 633 552 531 499 377 306
276 221 169 114 111 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 9986 10108 10213 12482 15012 15249
Size: 683 56 8760 122 105 2269 2530 237
Cuts at: 15249 17076 487
Size: 1827 3737
Fragments arranged by size:
8760 3737 2530 2269 1827 683 237 122
105 56
BtsI GCAGTG_nn'
Cuts at: 2929 4099 4926 6853 7810 8614 8995 10481 11156
Size: 1170 827 1927 957 804 381 1486 675
Cuts at: 11156 12583 14050 14780 17842 18636 18656 2929
Size: 1427 1467 730 3062 794 20 4599
Fragments arranged by size:
4599 3062 1927 1486 1467 1427 1170 957
827 804 794 730 675 381 20
ClaI AT'CG_AT
Cuts at: 1554 11771 12330 1554
Size: 10217 559 9550
Fragments arranged by size:
10217 9550 559
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2491 2526 529
Size: 35 1927 35 18329
Fragments arranged by size:
18329 1927 35 35
DraI TTT'AAA
Cuts at: 5148 12774 15990 17630 5148
Size: 7626 3216 1640 7844
Fragments arranged by size:
7844 7626 3216 1640
DraIII CAC_nnn'GTG
Cuts at: 10417 14833 16270 10417
Size: 4416 1437 14473
Fragments arranged by size:
14473 4416 1437
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10772 19333 809
Size: 507 9456 8561 1802
Fragments arranged by size:
9456 8561 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2437 2556 4977
Size: 54 1425 50 275 18 607 119 2421
Cuts at: 4977 6612 6618 6664 7411 7486 7510 9529 9660
Size: 1635 6 46 747 75 24 2019 131
Cuts at: 9660 10065 11004 11377 12454 14760 14899 15027 15342
Size: 405 939 373 1077 2306 139 128 315
Cuts at: 15342 15526 15670 15885 16146 17773 18662 20291 8
Size: 184 144 215 261 1627 889 1629 43
Fragments arranged by size:
2421 2306 2019 1635 1629 1627 1425 1077
939 889 747 607 405 373 315 275
261 215 184 144 139 131 128 119
75 54 50 46 43 24 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 9660 14899 17773 1537
Size: 275 7848 5239 2874 4090
Fragments arranged by size:
7848 5239 4090 2874 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3174 3758 4280 5714 7061 7284 8006 8341
Size: 1821 584 522 1434 1347 223 722 335
Cuts at: 8341 8708 8885 10304 10394 11454 11552 11621 12271
Size: 367 177 1419 90 1060 98 69 650
Cuts at: 12271 12780 13419 13793 14297 14528 14595 17547 18256
Size: 509 639 374 504 231 67 2952 709
Cuts at: 18256 1353
Size: 3423
Fragments arranged by size:
3423 2952 1821 1434 1419 1347 1060 722
709 650 639 584 522 509 504 374
367 335 231 223 177 98 90 69
67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6529 10565 11432 12461 12966 14241
Size: 1064 138 4940 4036 867 1029 505 1275
Cuts at: 14241 14690 17450 18913 19287 19433 387
Size: 449 2760 1463 374 146 1280
Fragments arranged by size:
4940 4036 2760 1463 1280 1275 1064 1029
867 505 449 374 146 138
EcoICRI GAG'CTC
Cuts at: 2725 5016 5646 6202 7150 7294 8334 9187 10732
Size: 2291 630 556 948 144 1040 853 1545
Cuts at: 10732 11575 13687 2725
Size: 843 2112 9364
Fragments arranged by size:
9364 2291 2112 1545 1040 948 853 843
630 556 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2498 2727 2997 3033 3509 3601
Size: 393 557 309 229 270 36 476 92
Cuts at: 3601 4159 4190 4211 4764 4840 5030 5170 5342
Size: 558 31 21 553 76 190 140 172
Cuts at: 5342 5507 6103 6209 6347 6442 6581 7816 8322
Size: 165 596 106 138 95 139 1235 506
Cuts at: 8322 8582 9056 9373 10307 10421 10595 10948 11531
Size: 260 474 317 934 114 174 353 583
Cuts at: 11531 11564 11888 11951 11953 12827 12830 13181 13280
Size: 33 324 63 2 874 3 351 99
Cuts at: 13280 13649 13703 14121 14517 14557 14558 14616 15095
Size: 369 54 418 396 40 1 58 479
Cuts at: 15095 15477 15515 15810 15887 15896 16091 16244 16883
Size: 382 38 295 77 9 195 153 639
Cuts at: 16883 18389 18986 19773 20041 1239
Size: 1506 597 787 268 1524
Fragments arranged by size:
1524 1506 1235 934 874 787 639 597
596 583 558 557 553 506 479 476
474 418 396 393 382 369 353 351
324 317 309 295 270 268 260 229
195 190 174 172 165 153 140 139
138 114 106 99 95 92 77 76
63 58 54 40 38 36 33 31
21 9 3 2 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2654 3572 5116 6053 6275 9056 10403 10775 13387
Size: 918 1544 937 222 2781 1347 372 2612
Cuts at: 13387 13730 14600 14684 14745 16322 16375 2654
Size: 343 870 84 61 1577 53 6605
Fragments arranged by size:
6605 2781 2612 1577 1544 1347 937 918
870 372 343 222 84 61 53
EcoO109I rG'GnC_Cy
Cuts at: 2182 2799 3565 4719 7233 7909 8152 9049 9061
Size: 617 766 1154 2514 676 243 897 12
Cuts at: 9061 10366 10501 10666 11406 11717 14020 14070 14884
Size: 1305 135 165 740 311 2303 50 814
Cuts at: 14884 15302 15871 16627 16786 2182
Size: 418 569 756 159 5722
Fragments arranged by size:
5722 2514 2303 1305 1154 897 814 766
756 740 676 617 569 418 311 243
165 159 135 50 12
EcoRI G'AATT_C
Cuts at: 10850 14129 17635 18013 10850
Size: 3279 3506 378 13163
Fragments arranged by size:
13163 3506 3279 378
EcoRV GAT'ATC
Cuts at: 11530 12924 15247 11530
Size: 1394 2323 16609
Fragments arranged by size:
16609 2323 1394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4739 5200 5695 6097 10660 10870 14326 14446 4739
Size: 461 495 402 4563 210 3456 120 10619
Fragments arranged by size:
10619 4563 3456 495 461 402 210 120
FspI TGC'GCA
Cuts at: 8139 13931 15944 18832 8139
Size: 5792 2013 2888 9633
Fragments arranged by size:
9633 5792 2888 2013
FspAI rTGC'GCAy
Cuts at: 13931 13931
Size: 20326
HaeII r_GCGC'y
Cuts at: 1678 4086 8118 8345 8876 9934 10746 10840 11820
Size: 2408 4032 227 531 1058 812 94 980
Cuts at: 11820 11938 12168 12633 13418 14311 17458 19473 1678
Size: 118 230 465 785 893 3147 2015 2531
Fragments arranged by size:
4032 3147 2531 2408 2015 1058 980 893
812 785 531 465 230 227 118 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 3947 3979 4508
Size: 32 135 32 905 32 1438 32 529
Cuts at: 4508 4531 4540 4563 5870 5902 5994 6026 6332
Size: 23 9 23 1307 32 92 32 306
Cuts at: 6332 6364 6869 6901 8071 8103 8165 8197 10029
Size: 32 505 32 1170 32 62 32 1832
Cuts at: 10029 10061 11102 11134 11701 11733 11753 11785 11837
Size: 32 1041 32 567 32 20 32 52
Cuts at: 11837 11869 12336 12368 13918 13950 14117 14149 14207
Size: 32 467 32 1550 32 167 32 58
Cuts at: 14207 14239 14717 14749 16287 16319 18967 18999 19041
Size: 32 478 32 1538 32 2648 32 42
Cuts at: 19041 19073 1373
Size: 32 2626
Fragments arranged by size:
2648 2626 1832 1550 1538 1438 1307 1170
1041 905 567 529 505 478 467 306
167 135 92 62 58 52 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 23 23
20 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3433 5271 9094 13341 15123 16011
Size: 988 281 1495 1838 3823 4247 1782 888
Cuts at: 16011 17670 17916 669
Size: 1659 246 3079
Fragments arranged by size:
4247 3823 3079 1838 1782 1659 1495 988
888 281 246
HindIII A'AGCT_T
Cuts at: 748 10986 748
Size: 10238 10088
HpaI GTT'AAC
Cuts at: 15123 17916 15123
Size: 2793 17533
KasI G'GCGC_C
Cuts at: 4082 8114 4082
Size: 4032 16294
KpnI G_GTAC'C
Cuts at: 3803 7270 9327 14986 17651 3803
Size: 3467 2057 5659 2665 6478
Fragments arranged by size:
6478 5659 3467 2665 2057
MfeI C'AATT_G
Cuts at: 17925 17925
Size: 20326
MluI A'CGCG_T
Cuts at: 12720 20202 12720
Size: 7482 12844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5293 7972 8883 9423 10683 13146 13523 13748
Size: 3988 2679 911 540 1260 2463 377 225
Cuts at: 13748 15470 16833 19440 19624 1305
Size: 1722 1363 2607 184 2007
Fragments arranged by size:
3988 2679 2607 2463 2007 1722 1363 1260
911 540 377 225 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2439 2558 4979 6614 6666
Size: 54 1425 343 607 119 2421 1635 52
Cuts at: 6666 7413 7512 9531 10067 11006 14762 15029 15344
Size: 747 99 2019 536 939 3756 267 315
Cuts at: 15344 15887 16148 10
Size: 543 261 4188
Fragments arranged by size:
4188 3756 2421 2019 1635 1425 939 747
607 543 536 343 315 267 261 119
99 54 52
MslI CAynn'nnrTG
Cuts at: 518 1166 3727 4131 5591 6377 6641 6818 8945
Size: 648 2561 404 1460 786 264 177 2127
Cuts at: 8945 9224 10073 10700 10886 11991 12043 12330 12353
Size: 279 849 627 186 1105 52 287 23
Cuts at: 12353 13065 13085 13334 13607 15923 16940 18284 18643
Size: 712 20 249 273 2316 1017 1344 359
Cuts at: 18643 18802 518
Size: 159 2042
Fragments arranged by size:
2561 2316 2127 2042 1460 1344 1105 1017
849 786 712 648 627 404 359 287
279 273 264 249 186 177 159 52
23 20
MspA1I CmG'CkG
Cuts at: 1606 3053 3438 3593 3727 4123 4850 5126 5734
Size: 1447 385 155 134 396 727 276 608
Cuts at: 5734 6359 7062 7129 7300 7644 7702 9626 9694
Size: 625 703 67 171 344 58 1924 68
Cuts at: 9694 9988 10591 11053 11843 12400 13140 13681 13719
Size: 294 603 462 790 557 740 541 38
Cuts at: 13719 14141 14863 15694 16052 16474 16876 17047 17117
Size: 422 722 831 358 422 402 171 70
Cuts at: 17117 17140 17272 17571 17594 17742 18419 19567 19812
Size: 23 132 299 23 148 677 1148 245
Cuts at: 19812 1606
Size: 2120
Fragments arranged by size:
2120 1924 1447 1148 831 790 740 727
722 703 677 625 608 603 557 541
462 422 422 402 396 385 358 344
299 294 276 245 171 171 155 148
134 132 70 68 67 58 38 23
23
NarI GG'CG_CC
Cuts at: 4083 8115 4083
Size: 4032 16294
NcoI C'CATG_G
Cuts at: 513 1065 2004 2381 6921 8844 12522 13270 15426
Size: 552 939 377 4540 1923 3678 748 2156
Cuts at: 15426 513
Size: 5413
Fragments arranged by size:
5413 4540 3678 2156 1923 939 748 552
377
NdeI CA'TA_TG
Cuts at: 387 9519 387
Size: 9132 11194
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 20326
NotI GC'GGCC_GC
Cuts at: 17773 17773
Size: 20326
NsiI A_TGCA'T
Cuts at: 4055 5000 10610 4055
Size: 945 5610 13771
Fragments arranged by size:
13771 5610 945
NspI r_CATG'y
Cuts at: 1132 1229 2030 4771 7552 12386 12643 13711 14180
Size: 97 801 2741 2781 4834 257 1068 469
Cuts at: 14180 14521 15437 15714 16763 17137 17503 17684 19229
Size: 341 916 277 1049 374 366 181 1545
Cuts at: 19229 1132
Size: 2229
Fragments arranged by size:
4834 2781 2741 2229 1545 1068 1049 916
801 469 374 366 341 277 257 181
97
PasI CC'CwG_GG
Cuts at: 1348 7654 7663 12981 1348
Size: 6306 9 5318 8693
Fragments arranged by size:
8693 6306 5318 9
PciI A'CATG_T
Cuts at: 15710 19225 15710
Size: 3515 16811
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2539 5662 7420 9538 9805 10990 13555 15679
Size: 1431 3123 1758 2118 267 1185 2565 2124
Cuts at: 15679 16308 1108
Size: 629 5126
Fragments arranged by size:
5126 3123 2565 2124 2118 1758 1431 1185
629 267
PfoI T'CCnGG_A
Cuts at: 2249 2592 3099 4194 4368 4402 4485 6225 6297
Size: 343 507 1095 174 34 83 1740 72
Cuts at: 6297 8130 8301 10776 12591 12846 2249
Size: 1833 171 2475 1815 255 9729
Fragments arranged by size:
9729 2475 1833 1815 1740 1095 507 343
255 174 171 83 72 34
PmeI GTTT'AAAC
Cuts at: 17630 17630
Size: 20326
PmlI CAC'GTG
Cuts at: 3796 8354 10709 13801 14047 17191 3796
Size: 4558 2355 3092 246 3144 6931
Fragments arranged by size:
6931 4558 3144 3092 2355 246
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2102 2134 3377 3409 9215 9247 18395 18427 19908
Size: 32 1243 32 5806 32 9148 32 1481
Cuts at: 19908 19940 2102
Size: 32 2488
Fragments arranged by size:
9148 5806 2488 1481 1243 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 3565 4719 7909 8152 9049 10501 11406 11717 14020
Size: 1154 3190 243 897 1452 905 311 2303
Cuts at: 14020 15871 16786 3565
Size: 1851 915 7105
Fragments arranged by size:
7105 3190 2303 1851 1452 1154 915 905
897 311 243
PshAI GACnn'nnGTC
Cuts at: 1613 3282 11397 13875 14387 1613
Size: 1669 8115 2478 512 7552
Fragments arranged by size:
8115 7552 2478 1669 512
PsiI TTA'TAA
Cuts at: 3369 5333 12200 17896 3369
Size: 1964 6867 5696 5799
Fragments arranged by size:
6867 5799 5696 1964
PspOMI G'GGCC_C
Cuts at: 2182 10366 10665 2182
Size: 8184 299 11843
Fragments arranged by size:
11843 8184 299
PspXI vC'TCGA_Gb
Cuts at: 7871 15041 7871
Size: 7170 13156
PstI C_TGCA'G
Cuts at: 830 2060 2089 2529 2778 3630 4574 4634 4669
Size: 1230 29 440 249 852 944 60 35
Cuts at: 4669 6261 6848 7037 7482 8402 8486 8973 9376
Size: 1592 587 189 445 920 84 487 403
Cuts at: 9376 10509 10903 11163 11172 11258 11322 11700 12054
Size: 1133 394 260 9 86 64 378 354
Cuts at: 12054 12381 13368 13482 14725 14787 15358 15855 16235
Size: 327 987 114 1243 62 571 497 380
Cuts at: 16235 16323 16574 17052 17678 830
Size: 88 251 478 626 3478
Fragments arranged by size:
3478 1592 1243 1230 1133 987 944 920
852 626 587 571 497 487 478 445
440 403 394 380 378 354 327 260
251 249 189 114 88 86 84 64
62 60 35 29 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 20326
PvuII CAG'CTG
Cuts at: 3053 3727 7062 7129 7702 9626 9694 9988 11053
Size: 674 3335 67 573 1924 68 294 1065
Cuts at: 11053 12400 13140 14141 16052 16474 17047 17117 17140
Size: 1347 740 1001 1911 422 573 70 23
Cuts at: 17140 17272 17571 17594 3053
Size: 132 299 23 5785
Fragments arranged by size:
5785 3335 1924 1911 1347 1065 1001 740
674 573 573 422 299 294 132 70
68 67 23 23
SacI G_AGCT'C
Cuts at: 2727 5018 5648 6204 7152 7296 8336 9189 10734
Size: 2291 630 556 948 144 1040 853 1545
Cuts at: 10734 11577 13689 2727
Size: 843 2112 9364
Fragments arranged by size:
9364 2291 2112 1545 1040 948 853 843
630 556 144
SacII CC_GC'GG
Cuts at: 4124 4851 5127 4124
Size: 727 276 19323
Fragments arranged by size:
19323 727 276
SalI G'TCGA_C
Cuts at: 1936 9092 17668 1936
Size: 7156 8576 4594
Fragments arranged by size:
8576 7156 4594
SanDI GG'GwC_CC
Cuts at: 4719 7909 11406 11717 4719
Size: 3190 3497 311 13328
Fragments arranged by size:
13328 3497 3190 311
SapI GCTCTTCn'nnn_
Cuts at: 5714 7284 8341 11552 14528 5714
Size: 1570 1057 3211 2976 11512
Fragments arranged by size:
11512 3211 2976 1570 1057
SbfI CC_TGCA'GG
Cuts at: 2529 8402 17678 2529
Size: 5873 9276 5177
Fragments arranged by size:
9276 5873 5177
ScaI AGT'ACT
Cuts at: 3488 6743 11360 11864 12071 18574 20286 3488
Size: 3255 4617 504 207 6503 1712 3528
Fragments arranged by size:
6503 4617 3528 3255 1712 504 207
SexAI A'CCwGG_T
Cuts at: 3567 4029 4311 6978 10413 14217 14838 14985 16821
Size: 462 282 2667 3435 3804 621 147 1836
Cuts at: 16821 3567
Size: 7072
Fragments arranged by size:
7072 3804 3435 2667 1836 621 462 282
147
SfcI C'TryA_G
Cuts at: 826 1045 2056 2085 2525 2774 2826 3626 4570
Size: 219 1011 29 440 249 52 800 944
Cuts at: 4570 4630 4665 6257 6844 7033 7478 7678 8398
Size: 60 35 1592 587 189 445 200 720
Cuts at: 8398 8482 8969 9372 9637 10505 10873 10899 11159
Size: 84 487 403 265 868 368 26 260
Cuts at: 11159 11168 11254 11318 11696 12050 12377 13364 13478
Size: 9 86 64 378 354 327 987 114
Cuts at: 13478 13652 14721 14783 15354 15851 16231 16319 16570
Size: 174 1069 62 571 497 380 88 251
Cuts at: 16570 17048 17056 17674 18809 19490 19681 826
Size: 478 8 618 1135 681 191 1471
Fragments arranged by size:
1592 1471 1135 1069 1011 987 944 868
800 720 681 618 587 571 497 487
478 445 440 403 380 378 368 354
327 265 260 251 249 219 200 191
189 174 114 88 86 84 64 62
60 52 35 29 26 9 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 2436 2436
Size: 20326
SfoI GGC'GCC
Cuts at: 4084 8116 4084
Size: 4032 16294
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 20326
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 20326
SmaI CCC'GGG
Cuts at: 1872 2199 2457 2764 3129 8826 11723 12269 17653
Size: 327 258 307 365 5697 2897 546 5384
Cuts at: 17653 1872
Size: 4545
Fragments arranged by size:
5697 5384 4545 2897 546 365 327 307
258
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3020 4409 4589 5810 5966 7228
Size: 197 926 1077 1389 180 1221 156 1262
Cuts at: 7228 7871 9125 14311 14552 14618 15041 15471 16532
Size: 643 1254 5186 241 66 423 430 1061
Cuts at: 16532 16922 17154 18430 19331 19593 820
Size: 390 232 1276 901 262 1553
Fragments arranged by size:
5186 1553 1389 1276 1262 1254 1221 1077
1061 926 901 643 430 423 390 262
241 232 197 180 156 66
SnaBI TAC'GTA
Cuts at: 493 18117 493
Size: 17624 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 20326
SphI G_CATG'C
Cuts at: 1132 12643 14180 15437 17684 1132
Size: 11511 1537 1257 2247 3774
Fragments arranged by size:
11511 3774 2247 1537 1257
SspI AAT'ATT
Cuts at: 5 52 14462 18250 5
Size: 47 14410 3788 2081
Fragments arranged by size:
14410 3788 2081 47
StuI AGG'CCT
Cuts at: 1517 2649 3873 4520 6869 8404 8696 14666 15080
Size: 1132 1224 647 2349 1535 292 5970 414
Cuts at: 15080 17201 1517
Size: 2121 4642
Fragments arranged by size:
5970 4642 2349 2121 1535 1224 1132 647
414 292
StyI C'CwwG_G
Cuts at: 513 1065 2004 2034 2381 2955 4000 4714 5117
Size: 552 939 30 347 574 1045 714 403
Cuts at: 5117 6072 6921 7408 8844 10746 10789 11229 12522
Size: 955 849 487 1436 1902 43 440 1293
Cuts at: 12522 12889 13270 13807 14763 14925 15426 16710 17765
Size: 367 381 537 956 162 501 1284 1055
Cuts at: 17765 513
Size: 3074
Fragments arranged by size:
3074 1902 1436 1293 1284 1055 1045 956
955 939 849 714 574 552 537 501
487 440 403 381 367 347 162 43
30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3680 3749 6033 6670 7430 8194 8928
Size: 1255 2100 69 2284 637 760 764 734
Cuts at: 8928 10576 10797 11261 11387 11575 13432 13865 14905
Size: 1648 221 464 126 188 1857 433 1040
Cuts at: 14905 14979 15732 17011 17162 18353 18370 18523 18708
Size: 74 753 1279 151 1191 17 153 185
Cuts at: 18708 19153 325
Size: 445 1498
Fragments arranged by size:
2284 2100 1857 1648 1498 1279 1255 1191
1040 764 760 753 734 637 464 445
433 221 188 185 153 151 126 74
69 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3486 3744 6741
Size: 274 80 33 51 1443 1509 258 2997
Cuts at: 6741 11241 11358 11475 11862 12069 13185 14424 14514
Size: 4500 117 117 387 207 1116 1239 90
Cuts at: 14514 15150 16944 18572 20284 96
Size: 636 1794 1628 1712 138
Fragments arranged by size:
4500 2997 1794 1712 1628 1509 1443 1239
1116 636 387 274 258 207 138 117
117 90 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3129 3161 3920 3952 5630 5662 6517 6549 8190
Size: 32 759 32 1678 32 855 32 1641
Cuts at: 8190 8222 8787 8819 10403 10435 10935 10951 10967
Size: 32 565 32 1584 32 500 16 16
Cuts at: 10967 10983 11148 11180 11604 11636 12707 12739 13154
Size: 16 165 32 424 32 1071 32 415
Cuts at: 13154 13186 14759 14791 20286 20318 3129
Size: 32 1573 32 5495 32 3137
Fragments arranged by size:
5495 3137 1678 1641 1584 1573 1071 855
759 565 500 424 415 165 32 32
32 32 32 32 32 32 32 32
32 32 32 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 1316 7924 10784 14024 14933 1316
Size: 6608 2860 3240 909 6709
Fragments arranged by size:
6709 6608 3240 2860 909
XbaI T'CTAG_A
Cuts at: 16078 17662 16078
Size: 1584 18742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2473 5663 6965 10345 11149 13219 13556 15596 2473
Size: 3190 1302 3380 804 2070 337 2040 7203
Fragments arranged by size:
7203 3380 3190 2070 2040 1302 804 337
XhoI C'TCGA_G
Cuts at: 1943 7871 15041 1943
Size: 5928 7170 7228
Fragments arranged by size:
7228 7170 5928
XmaI C'CCGG_G
Cuts at: 1870 2197 2455 2762 3127 8824 11721 12267 17651
Size: 327 258 307 365 5697 2897 546 5384
Cuts at: 17651 1870
Size: 4545
Fragments arranged by size:
5697 5384 4545 2897 546 365 327 307
258
XmnI GAAnn'nnTTC
Cuts at: 3656 4693 9944 10259 10924 13293 13313 14486 16394
Size: 1037 5251 315 665 2369 20 1173 1908
Cuts at: 16394 17523 18455 3656
Size: 1129 932 5527
Fragments arranged by size:
5527 5251 2369 1908 1173 1129 1037 932
665 315 20
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8316 9037 11470 11653
Size: 53 83 186 936 6782 721 2433 183
Cuts at: 11653 18134 276
Size: 6481 2468
Fragments arranged by size:
6782 6481 2468 2433 936 721 186 183
83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI BsiWI FseI NaeI NgoMIV NruI PacI PsrI
RsrII SrfI SwaI