Restriction map for
pFN21AE3553
(Circular) (Six-base) MAPSORT of: pFN21AE3553.seq Check: 3849 from: 1 to: 10603
pFN21AE3553 10603 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2377 5775 6859 2377
Size: 3398 1084 6121
Fragments arranged by size:
6121 3398 1084
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8413 278
Size: 53 83 186 936 6877 2468
Fragments arranged by size:
6877 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2976 3297 7719 7946 1937
Size: 1039 321 4422 227 4594
Fragments arranged by size:
4594 4422 1039 321 227
Acc65I G'GTAC_C
Cuts at: 3003 6872 7924 3003
Size: 3869 1052 5682
Fragments arranged by size:
5682 3869 1052
AclI AA'CG_TT
Cuts at: 1563 8730 9103 1563
Size: 7167 373 3063
Fragments arranged by size:
7167 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2268 3338 3596 4130 5938 6473 6627 6981
Size: 636 1070 258 534 1808 535 154 354
Cuts at: 6981 8666 10050 10318 1632
Size: 1685 1384 268 1917
Fragments arranged by size:
1917 1808 1685 1384 1070 636 535 534
354 268 258 154
AflII C'TTAA_G
Cuts at: 820 1017 5992 6595 820
Size: 197 4975 603 4828
Fragments arranged by size:
4975 4828 603 197
AflIII A'CryG_T
Cuts at: 1203 2979 7338 9502 10479 1203
Size: 1776 4359 2164 977 1327
Fragments arranged by size:
4359 2164 1776 1327 977
AgeI A'CCGG_T
Cuts at: 9434 9434
Size: 10603
AhdI GACnn_n'nnGTC
Cuts at: 9332 9332
Size: 10603
AleI CACnn'nnGTG
Cuts at: 1166 2585 1166
Size: 1419 9184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6168 1184
Size: 4984 5619
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2085 2117 2085
Size: 32 10571
AlwNI CAG_nnn'CTG
Cuts at: 2110 2392 5505 5660 6451 6664 6851 8346 9918
Size: 282 3113 155 791 213 187 1495 1572
Cuts at: 9918 2110
Size: 2795
Fragments arranged by size:
3113 2795 1572 1495 791 282 213 187
155
ApaI G_GGCC'C
Cuts at: 7846 7846
Size: 10603
ApaLI G'TGCA_C
Cuts at: 2831 3450 4557 8660 9816 2831
Size: 619 1107 4103 1156 3618
Fragments arranged by size:
4103 3618 1156 1107 619
ApoI r'AATT_y
Cuts at: 1493 3239 3440 3879 3892 3943 3962 5145 5978
Size: 1746 201 439 13 51 19 1183 833
Cuts at: 5978 6248 6299 6668 7912 8138 8290 10399 1493
Size: 270 51 369 1244 226 152 2109 1697
Fragments arranged by size:
2109 1746 1697 1244 1183 833 439 369
270 226 201 152 51 51 19 13
AseI AT'TA_AT
Cuts at: 160 1032 5456 6498 9157 160
Size: 872 4424 1042 2659 1606
Fragments arranged by size:
4424 2659 1606 1042 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10603
AvaI C'yCGr_G
Cuts at: 1870 1943 7134 7928 1870
Size: 73 5191 794 4545
Fragments arranged by size:
5191 4545 794 73
AvrII C'CTAG_G
Cuts at: 2492 5167 2492
Size: 2675 7928
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6761 6794 6864 6897 7407 7440 6761
Size: 33 70 33 510 33 9924
Fragments arranged by size:
9924 510 70 33 33 33
BamHI G'GATC_C
Cuts at: 2515 7933 8300 2515
Size: 5418 367 4818
Fragments arranged by size:
5418 4818 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2488 3003 6872 7636 7648
Size: 325 217 627 701 515 3869 764 12
Cuts at: 7648 7737 7924 9379 618
Size: 89 187 1455 1842
Fragments arranged by size:
3869 1842 1455 764 701 627 515 325
217 187 89 12
BanII G_rGCy'C
Cuts at: 1391 2544 3579 4301 5394 6866 7463 7846 7922
Size: 1153 1035 722 1093 1472 597 383 76
Cuts at: 7922 1391
Size: 4072
Fragments arranged by size:
4072 1472 1153 1093 1035 722 597 383
76
BbeI G_GCGC'C
Cuts at: 7741 7741
Size: 10603
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2256 2529 6461 928
Size: 971 357 273 3932 5070
Fragments arranged by size:
5070 3932 971 357 273
BbvCI CC'TCA_GC
Cuts at: 4664 4695 4664
Size: 31 10572
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4378 4412 8794 8828 1903
Size: 34 2441 34 4382 34 3678
Fragments arranged by size:
4382 3678 2441 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2004 3163 3610 3850 3859 4574 6762 7435 8495
Size: 1159 447 240 9 715 2188 673 1060
Cuts at: 8495 9705 2004
Size: 1210 2902
Fragments arranged by size:
2902 2188 1210 1159 1060 715 673 447
240 9
BclI T'GATC_A
Cuts at: 1548 4548 1548
Size: 3000 7603
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3686 7441 9214
Size: 107 122 71 631 605 2014 3755 1773
Cuts at: 9214 136
Size: 1525
Fragments arranged by size:
3755 2014 1773 1525 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4033 5010 5242 10598 4033
Size: 977 232 5356 4038
Fragments arranged by size:
5356 4038 977 232
BlpI GC'TnA_GC
Cuts at: 4708 8020 4708
Size: 3312 7291
Bme1580I G_kGCm'C
Cuts at: 1790 2835 3454 4021 4561 7653 7846 8664 9820
Size: 1045 619 567 540 3092 193 818 1156
Cuts at: 9820 1790
Size: 2573
Fragments arranged by size:
3092 2573 1156 1045 818 619 567 540
193
BmgBI CAC'GTC
Cuts at: 1318 2428 7581 7779 1318
Size: 1110 5153 198 4142
Fragments arranged by size:
5153 4142 1110 198
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2299 2467 2483
Size: 416 53 83 399 24 882 168 16
Cuts at: 2483 4749 5296 8334 9292 442
Size: 2266 547 3038 958 1753
Fragments arranged by size:
3038 2266 1753 958 882 547 416 399
168 83 53 24 16
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10603
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3385 7065 7516 7716 3385
Size: 3680 451 200 6272
Fragments arranged by size:
6272 3680 451 200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2678 4284 4640 7327 7470 7853 9263
Size: 893 546 1606 356 2687 143 383 1410
Cuts at: 9263 1239
Size: 2579
Fragments arranged by size:
2687 2579 1606 1410 893 546 383 356
143
Bpu10I CC'TnA_GC
Cuts at: 4568 4664 4695 5879 6763 4568
Size: 96 31 1184 884 8408
Fragments arranged by size:
8408 1184 884 96 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2740 6668 6687 7018 7060 8728 9593 9891 2740
Size: 3928 19 331 42 1668 865 298 3452
Fragments arranged by size:
3928 3452 1668 865 331 298 42 19
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6107 6706 7159 7897 9266 882
Size: 617 4608 599 453 738 1369 2219
Fragments arranged by size:
4608 2219 1369 738 617 599 453
BsaAI yAC'GTr
Cuts at: 493 1204 4060 4562 8394 493
Size: 711 2856 502 3832 2702
Fragments arranged by size:
3832 2856 2702 711 502
BsaBI GATnn'nnATC
Cuts at: 5631 10597 5631
Size: 4966 5637
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7738 8410 8792 9427
Size: 53 83 186 936 6205 672 382 635
Cuts at: 9427 275
Size: 1451
Fragments arranged by size:
6205 1451 936 672 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2415 2518 2761 4065 6205 9036 9434 9708 9855
Size: 103 243 1304 2140 2831 398 274 147
Cuts at: 9855 10266 2415
Size: 411 2752
Fragments arranged by size:
2831 2752 2140 1304 411 398 274 243
147 103
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2085 2115 4500 4530 5474
Size: 30 165 30 682 30 2385 30 944
Cuts at: 5474 5504 5582 5612 5996 6026 6311 6341 7468
Size: 30 78 30 384 30 285 30 1127
Cuts at: 7468 7498 7748 7778 1178
Size: 30 250 30 4003
Fragments arranged by size:
4003 2385 1127 944 682 384 285 250
165 78 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2666 4205 5606 5663 6925 7688 1186
Size: 1480 1539 1401 57 1262 763 4101
Fragments arranged by size:
4101 1539 1480 1401 1262 763 57
BseYI C'CCAG_C
Cuts at: 3169 4013 5235 6910 9806 10302 3169
Size: 844 1222 1675 2896 496 3470
Fragments arranged by size:
3470 2896 1675 1222 844 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2609 2934 5530 5753 6362 7254 7487
Size: 573 873 325 2596 223 609 892 233
Cuts at: 7487 7594 1163
Size: 107 4172
Fragments arranged by size:
4172 2596 892 873 609 573 325 233
223 107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2416 2851 8053 8814 9842 1540
Size: 275 186 415 435 5202 761 1028 2301
Fragments arranged by size:
5202 2301 1028 761 435 415 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 2544 2835 3454 3579 4050 4301 4561 5178
Size: 519 291 619 125 471 251 260 617
Cuts at: 5178 5394 5766 8664 8749 9820 2025
Size: 216 372 2898 85 1071 2808
Fragments arranged by size:
2898 2808 1071 619 617 519 471 372
291 260 251 216 125 85
BsmI GAATG_Cn'
Cuts at: 2881 3043 4206 6063 6572 8114 8207 2881
Size: 162 1163 1857 509 1542 93 5277
Fragments arranged by size:
5277 1857 1542 1163 509 162 93
BsmBI CGTCTCn'nnnn_
Cuts at: 7227 7227
Size: 10603
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2544 2835 3454 3579 4021 4050
Size: 399 235 519 291 619 125 442 29
Cuts at: 4050 4301 4561 5178 5394 5766 6866 7463 7653
Size: 251 260 617 216 372 1100 597 190
Cuts at: 7653 7846 7922 8664 8749 9820 1391
Size: 193 76 742 85 1071 2174
Fragments arranged by size:
2174 1100 1071 742 619 617 597 519
442 399 372 291 260 251 235 216
193 190 125 85 76 29
BspEI T'CCGG_A
Cuts at: 2518 6205 10266 2518
Size: 3687 4061 2855
Fragments arranged by size:
4061 3687 2855
BspHI T'CATG_A
Cuts at: 2358 3571 5256 5272 8492 10222 2358
Size: 1213 1685 16 3220 1730 2739
Fragments arranged by size:
3220 2739 1730 1685 1213 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2377 2943 3059 5775 6859 7958 844
Size: 1533 566 116 2716 1084 1099 3489
Fragments arranged by size:
3489 2716 1533 1099 1084 566 116
BsrBI CCG'CTC
Cuts at: 1394 1823 4084 5425 7726 8490 1394
Size: 429 2261 1341 2301 764 3507
Fragments arranged by size:
3507 2301 2261 1341 764 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2470 2514 2770 4719 9098 9272 65
Size: 1183 1222 44 256 1949 4379 174 1396
Fragments arranged by size:
4379 1949 1396 1222 1183 256 174 44
BsrFI r'CCGG_y
Cuts at: 1700 1810 2343 2694 7569 7633 7645 9247 9434
Size: 110 533 351 4875 64 12 1602 187
Cuts at: 9434 1700
Size: 2869
Fragments arranged by size:
4875 2869 1602 533 351 187 110 64
12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10603
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10603
BssSI C'ACGA_G
Cuts at: 2024 6828 8663 9675 2024
Size: 4804 1835 1012 2952
Fragments arranged by size:
4804 2952 1835 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6349 7213 794
Size: 5555 864 4184
Fragments arranged by size:
5555 4184 864
BstBI TT'CG_AA
Cuts at: 9407 9407
Size: 10603
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10603
BstXI CCAn_nnnn'nTGG
Cuts at: 3274 3433 3905 4651 4905 7810 7831 3274
Size: 159 472 746 254 2905 21 6046
Fragments arranged by size:
6046 2905 746 472 254 159 21
BstYI r'GATC_y
Cuts at: 1972 2106 2515 4033 5010 5242 7933 8300 8685
Size: 134 409 1518 977 232 2691 367 385
Cuts at: 8685 8702 10154 10240 10252 10598 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2691 1977 1518 1452 977 409 385 367
346 232 134 86 17 12
BstZ17I GTA'TAC
Cuts at: 2977 2977
Size: 10603
Bsu36I CC'TnA_GG
Cuts at: 2378 5714 7591 2378
Size: 3336 1877 5390
Fragments arranged by size:
5390 3336 1877
BtgI C'CryG_G
Cuts at: 513 1065 4449 7865 513
Size: 552 3384 3416 3251
Fragments arranged by size:
3416 3384 3251 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2911 6067 487
Size: 683 56 1685 3156 5023
Fragments arranged by size:
5023 3156 1685 683 56
BtsI GCAGTG_nn'
Cuts at: 2806 4439 7180 7258 7555 8119 8913 8933 2806
Size: 1633 2741 78 297 564 794 20 4476
Fragments arranged by size:
4476 2741 1633 794 564 297 78 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10603
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 7176 7211 529
Size: 35 6612 35 3921
Fragments arranged by size:
6612 3921 35 35
DraI TTT'AAA
Cuts at: 3976 5314 7907 3976
Size: 1338 2593 6672
Fragments arranged by size:
6672 2593 1338
DraIII CAC_nnn'GTG
Cuts at: 7253 7253
Size: 10603
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9610 809
Size: 507 8294 1802
Fragments arranged by size:
8294 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2696 3264 3307
Size: 54 1425 50 275 18 866 568 43
Cuts at: 3307 4641 6678 8050 8939 10568 8
Size: 1334 2037 1372 889 1629 43
Fragments arranged by size:
2037 1629 1425 1372 1334 889 866 568
275 54 50 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8050 1537
Size: 275 6238 4090
Fragments arranged by size:
6238 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2342 2618 3251 3561 4182 5399 8533 1353
Size: 989 276 633 310 621 1217 3134 3423
Fragments arranged by size:
3423 3134 1217 989 633 621 310 276
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5314 7692 9190 9564 9710 387
Size: 1064 138 3725 2378 1498 374 146 1280
Fragments arranged by size:
3725 2378 1498 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2542 3577 4299 5392 2542
Size: 1035 722 1093 7753
Fragments arranged by size:
7753 1093 1035 722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2268 2678 3338 3596 4130 4284
Size: 393 500 136 410 660 258 534 154
Cuts at: 4284 4640 5938 6473 6627 6981 7327 7470 7853
Size: 356 1298 535 154 354 346 143 383
Cuts at: 7853 8666 9263 10050 10318 1239
Size: 813 597 787 268 1524
Fragments arranged by size:
1524 1298 813 787 660 597 535 534
500 410 393 383 356 354 346 268
258 154 154 143 136
EcoNI CCTnn'n_nnAGG
Cuts at: 2648 2648
Size: 10603
EcoO109I rG'GnC_Cy
Cuts at: 4543 6184 7239 7842 7875 4543
Size: 1641 1055 603 33 7271
Fragments arranged by size:
7271 1641 1055 603 33
EcoRI G'AATT_C
Cuts at: 6299 7912 8290 6299
Size: 1613 378 8612
Fragments arranged by size:
8612 1613 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3718 5002 6778 3718
Size: 1284 1776 7543
Fragments arranged by size:
7543 1776 1284
FspI TGC'GCA
Cuts at: 7685 9109 7685
Size: 1424 9179
HaeII r_GCGC'y
Cuts at: 1678 2437 7741 9750 1678
Size: 759 5304 2009 2531
Fragments arranged by size:
5304 2531 2009 759
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2545 2577 3314 3346 4023
Size: 32 135 32 973 32 737 32 677
Cuts at: 4023 4055 6311 6343 7546 7578 9244 9276 9318
Size: 32 2256 32 1203 32 1666 32 42
Cuts at: 9318 9350 1373
Size: 32 2626
Fragments arranged by size:
2626 2256 1666 1203 973 737 677 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6736 6919 7947 8193 669
Size: 988 281 4798 183 1028 246 3079
Fragments arranged by size:
4798 3079 1028 988 281 246 183
HindIII A'AGCT_T
Cuts at: 748 5180 6220 6951 7036 748
Size: 4432 1040 731 85 4315
Fragments arranged by size:
4432 4315 1040 731 85
HpaI GTT'AAC
Cuts at: 8193 8193
Size: 10603
KasI G'GCGC_C
Cuts at: 7737 7737
Size: 10603
KpnI G_GTAC'C
Cuts at: 3007 6876 7928 3007
Size: 3869 1052 5682
Fragments arranged by size:
5682 3869 1052
MfeI C'AATT_G
Cuts at: 6420 8202 6420
Size: 1782 8821
MluI A'CGCG_T
Cuts at: 10479 10479
Size: 10603
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2664 5049 5941 6073 7211 7323 7542 7563
Size: 1359 2385 892 132 1138 112 219 21
Cuts at: 7563 7761 9717 9901 1305
Size: 198 1956 184 2007
Fragments arranged by size:
2385 2007 1956 1359 1138 892 219 198
184 132 112 21
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3266 3309 4643 10
Size: 54 1425 343 1434 43 1334 5970
Fragments arranged by size:
5970 1434 1425 1334 343 54 43
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 2585 3431 3591 4043 4404 5513
Size: 648 1101 318 846 160 452 361 1109
Cuts at: 5513 6086 8561 8920 9079 518
Size: 573 2475 359 159 2042
Fragments arranged by size:
2475 2042 1109 1101 846 648 573 452
361 359 318 160 159
MspA1I CmG'CkG
Cuts at: 1606 4200 6451 6914 7305 7867 8019 8696 9844
Size: 2594 2251 463 391 562 152 677 1148
Cuts at: 9844 10089 1606
Size: 245 2120
Fragments arranged by size:
2594 2251 2120 1148 677 562 463 391
245 152
NarI GG'CG_CC
Cuts at: 7738 7738
Size: 10603
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 10051
NdeI CA'TA_TG
Cuts at: 387 2071 387
Size: 1684 8919
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10603
NotI GC'GGCC_GC
Cuts at: 8050 8050
Size: 10603
NspI r_CATG'y
Cuts at: 1132 1229 2983 4403 5125 5158 7015 7342 7961
Size: 97 1754 1420 722 33 1857 327 619
Cuts at: 7961 9506 1132
Size: 1545 2229
Fragments arranged by size:
2229 1857 1754 1545 1420 722 619 327
97 33
PasI CC'CwG_GG
Cuts at: 1348 4375 1348
Size: 3027 7576
PciI A'CATG_T
Cuts at: 2979 7338 9502 2979
Size: 4359 2164 4080
Fragments arranged by size:
4359 4080 2164
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 10603
PfoI T'CCnGG_A
Cuts at: 2109 2370 4098 4344 4365 4728 2109
Size: 261 1728 246 21 363 7984
Fragments arranged by size:
7984 1728 363 261 246 21
PmeI GTTT'AAAC
Cuts at: 7907 7907
Size: 10603
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2049 2081 2085 2117 6186 6218 8672 8704 10185
Size: 32 4 32 4069 32 2454 32 1481
Cuts at: 10185 10217 2049
Size: 32 2435
Fragments arranged by size:
4069 2454 2435 1481 32 32 32 32
32 4
PpuMI rG'GwC_Cy
Cuts at: 4543 6184 4543
Size: 1641 8962
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10603
PsiI TTA'TAA
Cuts at: 3048 5007 5048 8173 3048
Size: 1959 41 3125 5478
Fragments arranged by size:
5478 3125 1959 41
PspOMI G'GGCC_C
Cuts at: 7842 7842
Size: 10603
PstI C_TGCA'G
Cuts at: 830 3855 4419 4434 4854 5067 6192 6541 7955
Size: 3025 564 15 420 213 1125 349 1414
Cuts at: 7955 830
Size: 3478
Fragments arranged by size:
3478 3025 1414 1125 564 420 349 213
15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10603
PvuII CAG'CTG
Cuts at: 6451 6914 7305 6451
Size: 463 391 9749
Fragments arranged by size:
9749 463 391
SacI G_AGCT'C
Cuts at: 2544 3579 4301 5394 2544
Size: 1035 722 1093 7753
Fragments arranged by size:
7753 1093 1035 722
SacII CC_GC'GG
Cuts at: 7868 7868
Size: 10603
SalI G'TCGA_C
Cuts at: 1936 7945 1936
Size: 6009 4594
SapI GCTCTTCn'nnn_
Cuts at: 2618 3251 5399 2618
Size: 633 2148 7822
Fragments arranged by size:
7822 2148 633
SbfI CC_TGCA'GG
Cuts at: 4419 7955 4419
Size: 3536 7067
ScaI AGT'ACT
Cuts at: 8851 10563 8851
Size: 1712 8891
SexAI A'CCwGG_T
Cuts at: 2484 2484
Size: 10603
SfcI C'TryA_G
Cuts at: 826 1045 2881 3851 4415 4430 4850 5063 5869
Size: 219 1836 970 564 15 420 213 806
Cuts at: 5869 6188 6537 7543 7951 9086 9767 9958 826
Size: 319 349 1006 408 1135 681 191 1471
Fragments arranged by size:
1836 1471 1135 1006 970 806 681 564
420 408 349 319 219 213 191 15
SfoI GGC'GCC
Cuts at: 7739 7739
Size: 10603
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10603
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10603
SmaI CCC'GGG
Cuts at: 1872 7930 1872
Size: 6058 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2719 5992 6595 6647 6702 7033
Size: 197 926 776 3273 603 52 55 331
Cuts at: 7033 7039 8707 9608 9870 820
Size: 6 1668 901 262 1553
Fragments arranged by size:
3273 1668 1553 926 901 776 603 331
262 197 55 52 6
SnaBI TAC'GTA
Cuts at: 493 8394 493
Size: 7901 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10603
SphI G_CATG'C
Cuts at: 1132 7961 1132
Size: 6829 3774
SspI AAT'ATT
Cuts at: 5 52 3128 3184 4875 6496 6973 8527 5
Size: 47 3076 56 1691 1621 477 1554 2081
Fragments arranged by size:
3076 2081 1691 1621 1554 477 56 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 10603
StyI C'CwwG_G
Cuts at: 513 1065 2492 3648 3661 5167 8042 513
Size: 552 1427 1156 13 1506 2875 3074
Fragments arranged by size:
3074 2875 1506 1427 1156 552 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2423 2837 3798 6276 6304 8630 8647
Size: 1255 843 414 961 2478 28 2326 17
Cuts at: 8647 8800 8985 9430 325
Size: 153 185 445 1498
Fragments arranged by size:
2478 2326 1498 1255 961 843 445 414
185 153 28 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4314 8849 10561
Size: 274 80 33 51 1443 2337 4535 1712
Cuts at: 10561 96
Size: 138
Fragments arranged by size:
4535 2337 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5996 6028 7115 7147 7468 7500 7748 7780 10563
Size: 32 1087 32 321 32 248 32 2783
Cuts at: 10563 10595 5996
Size: 32 6004
Fragments arranged by size:
6004 2783 1087 321 248 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10603
XbaI T'CTAG_A
Cuts at: 7939 7939
Size: 10603
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4399 4736 6815 7835 4399
Size: 337 2079 1020 7167
Fragments arranged by size:
7167 2079 1020 337
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10603
XmaI C'CCGG_G
Cuts at: 1870 7928 1870
Size: 6058 4545
XmnI GAAnn'nnTTC
Cuts at: 6068 6299 8732 6068
Size: 231 2433 7939
Fragments arranged by size:
7939 2433 231
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8411 276
Size: 53 83 186 936 6877 2468
Fragments arranged by size:
6877 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BfrBI BsiWI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SanDI SfiI SrfI SwaI