Restriction map for
pFN21AE3544
(Circular) (Six-base) MAPSORT of: pFN21AE3544.seq Check: 4819 from: 1 to: 5941
pFN21AE3544 5941 bp
With 182 enzymes: SgfI *
December 8, 2014 13:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 2558 4004 5388 5656 1632
Size: 635 291 1446 1384 268 1917
Fragments arranged by size:
1917 1446 1384 635 291 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5941
AlwNI CAG_nnn'CTG
Cuts at: 3008 3684 5256 3008
Size: 676 1572 3693
Fragments arranged by size:
3693 1572 676
ApaI G_GGCC'C
Cuts at: 2211 2260 2774 2211
Size: 49 514 5378
Fragments arranged by size:
5378 514 49
ApaLI G'TGCA_C
Cuts at: 2351 3998 5154 2351
Size: 1647 1156 3138
Fragments arranged by size:
3138 1647 1156
ApoI r'AATT_y
Cuts at: 1493 3155 3250 3476 3628 5737 1493
Size: 1662 95 226 152 2109 1697
Fragments arranged by size:
2109 1697 1662 226 152 95
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2135 2767 2894 2960 3062 3083 3266
Size: 73 192 632 127 66 102 21 183
Cuts at: 3266 1870
Size: 4545
Fragments arranged by size:
4545 632 192 183 127 102 73 66
21
BamHI G'GATC_C
Cuts at: 2064 3271 3638 2064
Size: 1207 367 4367
Fragments arranged by size:
4367 1207 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2570 2693 2986 3019 3047
Size: 325 217 627 783 123 293 33 28
Cuts at: 3047 3078 3203 3262 4717 618
Size: 31 125 59 1455 1842
Fragments arranged by size:
1842 1455 783 627 325 293 217 125
123 59 33 31 28
BanII G_rGCy'C
Cuts at: 1391 2136 2211 2260 2511 2774 2909 3042 3063
Size: 745 75 49 251 263 135 133 21
Cuts at: 3063 3260 1391
Size: 197 4072
Fragments arranged by size:
4072 745 263 251 197 135 133 75
49 21
BbeI G_GCGC'C
Cuts at: 2574 2697 3082 3207 2574
Size: 123 385 125 5308
Fragments arranged by size:
5308 385 125 123
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2026 928
Size: 971 127 4843
Fragments arranged by size:
4843 971 127
BbvCI CC'TCA_GC
Cuts at: 2754 2761 2754
Size: 7 5934
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4132 4166 1903
Size: 34 2195 34 3678
Fragments arranged by size:
3678 2195 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3017 4552 136
Size: 107 122 71 631 605 1345 1535 1525
Fragments arranged by size:
1535 1525 1345 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2211 2260 2355 2774 2989 4002 5158 1790
Size: 421 49 95 419 215 1013 1156 2573
Fragments arranged by size:
2573 1156 1013 421 419 215 95 49
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3672 4630 442
Size: 416 53 83 399 24 2255 958 1753
Fragments arranged by size:
2255 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2323 4601 1239
Size: 1084 2278 2579
Fragments arranged by size:
2579 2278 1084
Bpu10I CC'TnA_GC
Cuts at: 2754 2761 3036 2754
Size: 7 275 5659
Fragments arranged by size:
5659 275 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2345 4066 4931 5229 2345
Size: 1721 865 298 3057
Fragments arranged by size:
3057 1721 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2891 4604 882
Size: 617 1392 1713 2219
Fragments arranged by size:
2219 1713 1392 617
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2571 2694 2811 3079
Size: 53 83 186 936 1038 123 117 268
Cuts at: 3079 3204 3748 4130 4765 275
Size: 125 544 382 635 1451
Fragments arranged by size:
1451 1038 936 635 544 382 268 186
125 123 117 83 53
BsaWI w'CCGG_w
Cuts at: 4374 4772 5046 5193 5604 4374
Size: 398 274 147 411 4711
Fragments arranged by size:
4711 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5716
Fragments arranged by size:
5716 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2311 2818 2879 3043 1186
Size: 967 158 507 61 164 4084
Fragments arranged by size:
4084 967 507 164 158 61
BseYI C'CCAG_C
Cuts at: 2918 3068 3191 3209 5144 5640 2918
Size: 150 123 18 1935 496 3219
Fragments arranged by size:
3219 1935 496 150 123 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 1163
Size: 573 582 4786
Fragments arranged by size:
4786 582 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2043 3391 4152 5180 1540
Size: 275 186 42 1348 761 1028 2301
Fragments arranged by size:
2301 1348 1028 761 275 186 42
BsiHKAI G_wGCw'C
Cuts at: 2355 2943 4002 4087 5158 2355
Size: 588 1059 85 1071 3138
Fragments arranged by size:
3138 1071 1059 588 85
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
BsmBI CGTCTCn'nnnn_
Cuts at: 2462 2462
Size: 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2211 2260 2355 2511 2774 2909
Size: 399 346 75 49 95 156 263 135
Cuts at: 2909 2943 2989 3042 3063 3260 4002 4087 5158
Size: 34 46 53 21 197 742 85 1071
Cuts at: 5158 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 346 263 197 156
135 95 85 75 53 49 46 34
21
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3296 844
Size: 2452 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 3828 1394
Size: 429 206 1799 3507
Fragments arranged by size:
3507 1799 429 206
BsrDI GCAATG_nn'
Cuts at: 65 1248 2163 4436 4610 65
Size: 1183 915 2273 174 1396
Fragments arranged by size:
2273 1396 1183 915 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2355 3011 3044 4585 4772 1700
Size: 110 545 656 33 1541 187 2869
Fragments arranged by size:
2869 1541 656 545 187 110 33
BsrGI T'GTAC_A
Cuts at: 96 2706 96
Size: 2610 3331
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 2175 4745 2175
Size: 2570 3371
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstYI r'GATC_y
Cuts at: 1972 2064 2673 3271 3638 4023 4040 5492 5578
Size: 92 609 598 367 385 17 1452 86
Cuts at: 5578 5590 5936 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 609 598 385 367 346 92
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2994 3168 2994
Size: 174 5767
BtgI C'CryG_G
Cuts at: 513 1065 2004 2501 3027 513
Size: 552 939 497 526 3427
Fragments arranged by size:
3427 939 552 526 497
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5202
Fragments arranged by size:
5202 683 56
BtsI GCAGTG_nn'
Cuts at: 2025 3457 4251 4271 2025
Size: 1432 794 20 3695
Fragments arranged by size:
3695 1432 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2787 3388
Size: 54 1425 50 275 18 210 747 601
Cuts at: 3388 4277 5906 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 747 601 275 210 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2040 3388 1537
Size: 275 228 1348 4090
Fragments arranged by size:
4090 1348 275 228
EarI CTCTTCn'nnn_
Cuts at: 1353 2210 3871 1353
Size: 857 1661 3423
Fragments arranged by size:
3423 1661 857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2077 2363 2666 2706 2902 4528
Size: 1064 138 488 286 303 40 196 1626
Cuts at: 4528 4902 5048 387
Size: 374 146 1280
Fragments arranged by size:
1626 1280 1064 488 374 303 286 196
146 138 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2323 2558 4004 4601 5388 5656
Size: 393 635 56 235 1446 597 787 268
Cuts at: 5656 1239
Size: 1524
Fragments arranged by size:
1524 1446 787 635 597 393 268 235
56
EcoNI CCTnn'n_nnAGG
Cuts at: 2246 2246
Size: 5941
EcoO109I rG'GnC_Cy
Cuts at: 2222 2269 2771 2222
Size: 47 502 5392
Fragments arranged by size:
5392 502 47
EcoRI G'AATT_C
Cuts at: 3155 3250 3628 3155
Size: 95 378 5468
Fragments arranged by size:
5468 378 95
FspI TGC'GCA
Cuts at: 2477 4447 2477
Size: 1970 3971
FspAI rTGC'GCAy
Cuts at: 2477 2477
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2313 2559 2574 2594 2697 3082 3207 5088
Size: 635 246 15 20 103 385 125 1881
Cuts at: 5088 1678
Size: 2531
Fragments arranged by size:
2531 1881 635 385 246 125 103 20
15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4582 4614 4656 4688 1373
Size: 32 135 32 3010 32 42 32 2626
Fragments arranged by size:
3010 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3285 3531 669
Size: 988 281 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KasI G'GCGC_C
Cuts at: 2570 2693 3078 3203 2570
Size: 123 385 125 5308
Fragments arranged by size:
5308 385 125 123
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5055 5239 1305
Size: 3750 184 2007
Fragments arranged by size:
3750 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4119
Fragments arranged by size:
4119 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3899 4258 4417 518
Size: 648 2733 359 159 2042
Fragments arranged by size:
2733 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2379 2503 2922 3005 3209 3357 4034 5182
Size: 773 124 419 83 204 148 677 1148
Cuts at: 5182 5427 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 773 677 419 245 204 148
124 83
NaeI GCC'GGC
Cuts at: 3013 3013
Size: 5941
NarI GG'CG_CC
Cuts at: 2571 2694 3079 3204 2571
Size: 123 385 125 5308
Fragments arranged by size:
5308 385 125 123
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4450
Fragments arranged by size:
4450 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NgoMIV G'CCGG_C
Cuts at: 3011 3011
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 3299 4844 1132
Size: 97 2070 1545 2229
Fragments arranged by size:
2229 2070 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2774 1348
Size: 1426 4515
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5941
PfoI T'CCnGG_A
Cuts at: 2148 2148
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2222 2222
Size: 5941
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 2207 2256 2770 2207
Size: 49 514 5378
Fragments arranged by size:
5378 514 49
PstI C_TGCA'G
Cuts at: 830 2020 3293 830
Size: 1190 1273 3478
Fragments arranged by size:
3478 1273 1190
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2922 3005 2922
Size: 83 5858
SacII CC_GC'GG
Cuts at: 2504 2504
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SanDI GG'GwC_CC
Cuts at: 2222 2222
Size: 5941
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SexAI A'CCwGG_T
Cuts at: 2088 2088
Size: 5941
SfcI C'TryA_G
Cuts at: 826 1045 2016 3289 4424 5105 5296 826
Size: 219 971 1273 1135 681 191 1471
Fragments arranged by size:
1471 1273 1135 971 681 219 191
SfoI GGC'GCC
Cuts at: 2572 2695 3080 3205 2572
Size: 123 385 125 5308
Fragments arranged by size:
5308 385 125 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 3044 1700
Size: 1344 4597
SmaI CCC'GGG
Cuts at: 1872 2769 3268 1872
Size: 897 499 4545
Fragments arranged by size:
4545 897 499
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2360 4045 4946 5208 820
Size: 197 926 417 1685 901 262 1553
Fragments arranged by size:
1685 1553 926 901 417 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 2759 1517
Size: 1242 4699
StyI C'CwwG_G
Cuts at: 513 1065 2004 3380 513
Size: 552 939 1376 3074
Fragments arranged by size:
3074 1376 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3968 3985 4138 4323 4768 325
Size: 1255 2388 17 153 185 445 1498
Fragments arranged by size:
2388 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 2706 4187
Size: 274 80 33 51 1443 141 588 1481
Cuts at: 4187 5899 96
Size: 1712 138
Fragments arranged by size:
1712 1481 1443 588 274 141 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2375 2407 5901 5933 2375
Size: 32 3494 32 2383
Fragments arranged by size:
3494 2383 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2767 3266 1870
Size: 897 499 4545
Fragments arranged by size:
4545 897 499
XmnI GAAnn'nnTTC
Cuts at: 4070 4070
Size: 5941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SapI SfiI SrfI SwaI XcmI