Restriction map for
pFN21AE3541
(Circular) (Six-base) MAPSORT of: pFN21AE3541.seq Check: 6316 from: 1 to: 5590
pFN21AE3541 5590 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3400 278
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2388 2622 2933 1937
Size: 451 234 311 4594
Fragments arranged by size:
4594 451 311 234
Acc65I G'GTAC_C
Cuts at: 2363 2911 2363
Size: 548 5042
AclI AA'CG_TT
Cuts at: 1563 3717 4090 1563
Size: 2154 373 3063
Fragments arranged by size:
3063 2154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3653 5037 5305 1632
Size: 2021 1384 268 1917
Fragments arranged by size:
2021 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5393
AflIII A'CryG_T
Cuts at: 1203 4489 5466 1203
Size: 3286 977 1327
Fragments arranged by size:
3286 1327 977
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 5590
AleI CACnn'nnGTG
Cuts at: 1166 2531 2702 1166
Size: 1365 171 4054
Fragments arranged by size:
4054 1365 171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5590
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2306 2338 2306
Size: 32 5558
AlwNI CAG_nnn'CTG
Cuts at: 3333 4905 3333
Size: 1572 4018
ApaLI G'TGCA_C
Cuts at: 3647 4803 3647
Size: 1156 4434
ApoI r'AATT_y
Cuts at: 1493 2899 3125 3277 5386 1493
Size: 1406 226 152 2109 1697
Fragments arranged by size:
2109 1697 1406 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4144 160
Size: 872 3112 1606
Fragments arranged by size:
3112 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2915 1870
Size: 73 972 4545
Fragments arranged by size:
4545 972 73
AvrII C'CTAG_G
Cuts at: 2434 2434
Size: 5590
BamHI G'GATC_C
Cuts at: 2920 3287 2920
Size: 367 5223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2354 2363 2774 2794 2911
Size: 325 217 627 567 9 411 20 117
Cuts at: 2911 4366 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 567 411 325 217 117
20 9
BanII G_rGCy'C
Cuts at: 1391 2250 2909 1391
Size: 859 659 4072
Fragments arranged by size:
4072 859 659
BbeI G_GCGC'C
Cuts at: 2358 2778 2358
Size: 420 5170
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4619
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3781 3815 1903
Size: 34 1844 34 3678
Fragments arranged by size:
3678 1844 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2867 3482 4692 2867
Size: 615 1210 3765
Fragments arranged by size:
3765 1210 615
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5590
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2138 2439 4201
Size: 107 122 71 631 605 466 301 1762
Cuts at: 4201 136
Size: 1525
Fragments arranged by size:
1762 1525 631 605 466 301 122 107
71
BglII A'GATC_T
Cuts at: 5585 5585
Size: 5590
BlpI GC'TnA_GC
Cuts at: 2552 3007 2552
Size: 455 5135
Bme1580I G_kGCm'C
Cuts at: 1790 3651 4807 1790
Size: 1861 1156 2573
Fragments arranged by size:
2573 1861 1156
BmgBI CAC'GTC
Cuts at: 1318 2587 1318
Size: 1269 4321
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3321 4279 442
Size: 416 53 83 399 24 1904 958 1753
Fragments arranged by size:
1904 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2264 4250 1239
Size: 1025 1986 2579
Fragments arranged by size:
2579 1986 1025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3715 4580 4878 3715
Size: 865 298 4427
Fragments arranged by size:
4427 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 2369 4253 882
Size: 617 504 366 1884 2219
Fragments arranged by size:
2219 1884 617 504 366
BsaAI yAC'GTr
Cuts at: 493 1204 2223 3381 493
Size: 711 1019 1158 2702
Fragments arranged by size:
2702 1158 1019 711
BsaBI GATnn'nnATC
Cuts at: 5584 5584
Size: 5590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2355 2425 2775 3397
Size: 53 83 186 936 822 70 350 622
Cuts at: 3397 3779 4414 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 822 635 622 382 350 186
83 70 53
BsaWI w'CCGG_w
Cuts at: 4023 4421 4695 4842 5253 4023
Size: 398 274 147 411 4360
Fragments arranged by size:
4360 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5365
Fragments arranged by size:
5365 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 1186
Size: 1009 4581
BseYI C'CCAG_C
Cuts at: 2146 2668 2867 4793 5289 2146
Size: 522 199 1926 496 2447
Fragments arranged by size:
2447 1926 522 496 199
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2142 1163
Size: 573 406 4611
Fragments arranged by size:
4611 573 406
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2209 2469 3040 3801 4829 1540
Size: 275 186 208 260 571 761 1028 2301
Fragments arranged by size:
2301 1028 761 571 275 260 208 186
BsiHKAI G_wGCw'C
Cuts at: 3651 3736 4807 3651
Size: 85 1071 4434
Fragments arranged by size:
4434 1071 85
BsmI GAATG_Cn'
Cuts at: 3101 3194 3101
Size: 93 5497
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2909 3651 3736 4807 1391
Size: 399 460 659 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 659 460 399 85
BspEI T'CCGG_A
Cuts at: 5253 5253
Size: 5590
BspHI T'CATG_A
Cuts at: 3479 5209 3479
Size: 1730 3860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2021 2375 2945 844
Size: 1177 354 570 3489
Fragments arranged by size:
3489 1177 570 354
BsrBI CCG'CTC
Cuts at: 1394 1823 2551 3477 1394
Size: 429 728 926 3507
Fragments arranged by size:
3507 926 728 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2242 4085 4259 65
Size: 1183 994 1843 174 1396
Fragments arranged by size:
1843 1396 1183 994 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 2445 2556 4234 4421 1700
Size: 110 208 427 111 1678 187 2869
Fragments arranged by size:
2869 1678 427 208 187 111 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5590
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5590
BssSI C'ACGA_G
Cuts at: 3650 4662 3650
Size: 1012 4578
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2769 794
Size: 1975 3615
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5590
BstXI CCAn_nnnn'nTGG
Cuts at: 2704 2837 2867 2704
Size: 133 30 5427
Fragments arranged by size:
5427 133 30
BstYI r'GATC_y
Cuts at: 1972 2920 3287 3672 3689 5141 5227 5239 5585
Size: 948 367 385 17 1452 86 12 346
Cuts at: 5585 1972
Size: 1977
Fragments arranged by size:
1977 1452 948 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2287 2293 2358 2419 2875 513
Size: 552 939 283 6 65 61 456 3228
Fragments arranged by size:
3228 939 552 456 283 65 61 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4851
Fragments arranged by size:
4851 683 56
BtsI GCAGTG_nn'
Cuts at: 2159 2639 2767 3106 3900 3920 2159
Size: 480 128 339 794 20 3829
Fragments arranged by size:
3829 794 480 339 128 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5555
DraI TTT'AAA
Cuts at: 2894 2894
Size: 5590
DraIII CAC_nnn'GTG
Cuts at: 2333 2333
Size: 5590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4597 809
Size: 507 3281 1802
Fragments arranged by size:
3281 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2290 2658 2712
Size: 54 1425 50 275 18 460 368 54
Cuts at: 2712 3037 3926 5555 8
Size: 325 889 1629 43
Fragments arranged by size:
1629 1425 889 460 368 325 275 54
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3037 1537
Size: 275 1225 4090
Fragments arranged by size:
4090 1225 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 3520 1353
Size: 692 1475 3423
Fragments arranged by size:
3423 1475 692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2489 4177 4551 4697 387
Size: 1064 138 900 1688 374 146 1280
Fragments arranged by size:
1688 1280 1064 900 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 3653 4250 5037 5305 1239
Size: 393 632 1389 597 787 268 1524
Fragments arranged by size:
1524 1389 787 632 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2081 2653 2081
Size: 572 5018
EcoRI G'AATT_C
Cuts at: 2899 3277 2899
Size: 378 5212
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 5590
FspI TGC'GCA
Cuts at: 4096 4096
Size: 5590
HaeII r_GCGC'y
Cuts at: 1678 2358 2606 2691 2778 4737 1678
Size: 680 248 85 87 1959 2531
Fragments arranged by size:
2531 1959 680 248 87 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373
Size: 32 135 32 2659 32 42 32 2626
Fragments arranged by size:
2659 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2934 3180 669
Size: 988 281 996 246 3079
Fragments arranged by size:
3079 996 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5590
HpaI GTT'AAC
Cuts at: 3180 3180
Size: 5590
KasI G'GCGC_C
Cuts at: 2354 2774 2354
Size: 420 5170
KpnI G_GTAC'C
Cuts at: 2367 2915 2367
Size: 548 5042
MfeI C'AATT_G
Cuts at: 3189 3189
Size: 5590
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4704 4888 1305
Size: 3399 184 2007
Fragments arranged by size:
3399 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2660 10
Size: 54 1425 343 828 2940
Fragments arranged by size:
2940 1425 828 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2309 2531 2702 3548 3907 4066 518
Size: 648 1143 222 171 846 359 159 2042
Fragments arranged by size:
2042 1143 846 648 359 222 171 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2190 2295 2360 2808 2814 3006 3683
Size: 426 158 105 65 448 6 192 677
Cuts at: 3683 4831 5076 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 448 426 245 192 158
105 65 6
NaeI GCC'GGC
Cuts at: 2447 2558 2447
Size: 111 5479
NarI GG'CG_CC
Cuts at: 2355 2775 2355
Size: 420 5170
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4099
Fragments arranged by size:
4099 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5590
NgoMIV G'CCGG_C
Cuts at: 2445 2556 2445
Size: 111 5479
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5590
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NspI r_CATG'y
Cuts at: 1132 1229 2598 2767 2948 4493 1132
Size: 97 1369 169 181 1545 2229
Fragments arranged by size:
2229 1545 1369 181 169 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5590
PciI A'CATG_T
Cuts at: 4489 4489
Size: 5590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2171 1108
Size: 1063 4527
PfoI T'CCnGG_A
Cuts at: 2118 2829 2118
Size: 711 4879
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 5172 5204 3659
Size: 32 1481 32 4045
Fragments arranged by size:
4045 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2081 2081
Size: 5590
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5590
PsiI TTA'TAA
Cuts at: 3160 3160
Size: 5590
PstI C_TGCA'G
Cuts at: 830 2271 2322 2342 2813 2819 2942 830
Size: 1441 51 20 471 6 123 3478
Fragments arranged by size:
3478 1441 471 123 51 20 6
PvuI CG_AT'CG
Cuts at: 2001 2469 2001
Size: 468 5122
PvuII CAG'CTG
Cuts at: 2814 2814
Size: 5590
RsrII CG'GwC_CG
Cuts at: 2546 2546
Size: 5590
SacII CC_GC'GG
Cuts at: 2296 2361 2296
Size: 65 5525
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SanDI GG'GwC_CC
Cuts at: 2081 2081
Size: 5590
SbfI CC_TGCA'GG
Cuts at: 2942 2942
Size: 5590
ScaI AGT'ACT
Cuts at: 3838 5550 3838
Size: 1712 3878
SfcI C'TryA_G
Cuts at: 826 1045 2267 2318 2338 2809 2815 2938 4073
Size: 219 1222 51 20 471 6 123 1135
Cuts at: 4073 4754 4945 826
Size: 681 191 1471
Fragments arranged by size:
1471 1222 1135 681 471 219 191 123
51 20 6
SfoI GGC'GCC
Cuts at: 2356 2776 2356
Size: 420 5170
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2917 1872
Size: 1045 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3694 4595 4857 820
Size: 197 926 1751 901 262 1553
Fragments arranged by size:
1751 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3381 493
Size: 2888 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2598 2767 2948 1132
Size: 1466 169 181 3774
Fragments arranged by size:
3774 1466 181 169
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5590
StyI C'CwwG_G
Cuts at: 513 1065 2004 2434 3029 513
Size: 552 939 430 595 3074
Fragments arranged by size:
3074 939 595 552 430
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2163 2492 3617 3634 3787 3972 4417
Size: 1255 583 329 1125 17 153 185 445
Cuts at: 4417 325
Size: 1498
Fragments arranged by size:
1498 1255 1125 583 445 329 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3836 5548 96
Size: 274 80 33 51 1443 1859 1712 138
Fragments arranged by size:
1859 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2272 2304 2709 2741 5550 5582 2272
Size: 32 405 32 2809 32 2280
Fragments arranged by size:
2809 2280 405 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5590
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2172 2172
Size: 5590
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5590
XmaI C'CCGG_G
Cuts at: 1870 2915 1870
Size: 1045 4545
XmnI GAAnn'nnTTC
Cuts at: 3719 3719
Size: 5590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3398 276
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI
PsrI SacI SapI SexAI SfiI SrfI SwaI