Restriction map for
pFN21AE3537
(Circular) (Six-base) MAPSORT of: pFN21AE3537.seq Check: 5961 from: 1 to: 6223
pFN21AE3537 6223 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2215 2215
Size: 6223
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4033 278
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 3566 1937
Size: 1002 627 4594
Fragments arranged by size:
4594 1002 627
Acc65I G'GTAC_C
Cuts at: 3544 3544
Size: 6223
AclI AA'CG_TT
Cuts at: 1563 4350 4723 1563
Size: 2787 373 3063
Fragments arranged by size:
3063 2787 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4286 5670 5938 1632
Size: 2654 1384 268 1917
Fragments arranged by size:
2654 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6026
AflIII A'CryG_T
Cuts at: 1203 5122 6099 1203
Size: 3919 977 1327
Fragments arranged by size:
3919 1327 977
AgeI A'CCGG_T
Cuts at: 5054 5054
Size: 6223
AhdI GACnn_n'nnGTC
Cuts at: 4952 4952
Size: 6223
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6223
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6223
AlwNI CAG_nnn'CTG
Cuts at: 2662 3966 5538 2662
Size: 1304 1572 3347
Fragments arranged by size:
3347 1572 1304
ApaLI G'TGCA_C
Cuts at: 2015 4280 5436 2015
Size: 2265 1156 2802
Fragments arranged by size:
2802 2265 1156
ApoI r'AATT_y
Cuts at: 1493 2075 2344 3532 3758 3910 6019 1493
Size: 582 269 1188 226 152 2109 1697
Fragments arranged by size:
2109 1697 1188 582 269 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2874 4777 160
Size: 872 1842 1903 1606
Fragments arranged by size:
1903 1842 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
AvaI C'yCGr_G
Cuts at: 1870 1943 2700 2984 3387 3548 1870
Size: 73 757 284 403 161 4545
Fragments arranged by size:
4545 757 403 284 161 73
AvrII C'CTAG_G
Cuts at: 2455 2455
Size: 6223
BamHI G'GATC_C
Cuts at: 3215 3553 3920 3215
Size: 338 367 5518
Fragments arranged by size:
5518 367 338
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2638 3435 3544 4999 618
Size: 325 217 627 851 797 109 1455 1842
Fragments arranged by size:
1842 1455 851 797 627 325 217 109
BanII G_rGCy'C
Cuts at: 1391 2622 2685 3000 3045 3466 3542 1391
Size: 1231 63 315 45 421 76 4072
Fragments arranged by size:
4072 1231 421 315 76 63 45
BbeI G_GCGC'C
Cuts at: 3439 3439
Size: 6223
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2202 2558 928
Size: 971 303 356 4593
Fragments arranged by size:
4593 971 356 303
BbvCI CC'TCA_GC
Cuts at: 3480 3480
Size: 6223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4414 4448 1903
Size: 34 2477 34 3678
Fragments arranged by size:
3678 2477 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4115 5325 4115
Size: 1210 5013
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6223
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4834 136
Size: 107 122 71 631 605 3162 1525
Fragments arranged by size:
3162 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6218 6218
Size: 6223
BlpI GC'TnA_GC
Cuts at: 3068 3640 3068
Size: 572 5651
Bme1580I G_kGCm'C
Cuts at: 1790 2019 2643 3174 4284 5440 1790
Size: 229 624 531 1110 1156 2573
Fragments arranged by size:
2573 1156 1110 624 531 229
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6223
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3136 3247 3404
Size: 416 53 83 399 24 1719 111 157
Cuts at: 3404 3954 4912 442
Size: 550 958 1753
Fragments arranged by size:
1753 1719 958 550 416 399 157 111
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3480 3480
Size: 6223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2005 2204 3097 4883 1239
Size: 766 199 893 1786 2579
Fragments arranged by size:
2579 1786 893 766 199
Bpu10I CC'TnA_GC
Cuts at: 3480 3480
Size: 6223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2741 3450 4348 5213 5511 2741
Size: 709 898 865 298 3453
Fragments arranged by size:
3453 898 865 709 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4886 882
Size: 617 3387 2219
Fragments arranged by size:
3387 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2635 4014 493
Size: 711 1431 1379 2702
Fragments arranged by size:
2702 1431 1379 711
BsaBI GATnn'nnATC
Cuts at: 6217 6217
Size: 6223
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3436 4030 4412 5047
Size: 53 83 186 936 1903 594 382 635
Cuts at: 5047 275
Size: 1451
Fragments arranged by size:
1903 1451 936 635 594 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2340 4656 5054 5328 5475 5886 2340
Size: 2316 398 274 147 411 2677
Fragments arranged by size:
2677 2316 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5998
Fragments arranged by size:
5998 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3419 1186
Size: 2233 3990
BseYI C'CCAG_C
Cuts at: 2798 3306 5426 5922 2798
Size: 508 2120 496 3099
Fragments arranged by size:
3099 2120 508 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2591 1163
Size: 573 855 4795
Fragments arranged by size:
4795 855 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3673 4434 5462 1540
Size: 275 186 1672 761 1028 2301
Fragments arranged by size:
2301 1672 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2019 2685 3000 3466 4284 4369 5440 2019
Size: 666 315 466 818 85 1071 2802
Fragments arranged by size:
2802 1071 818 666 466 315 85
BsmI GAATG_Cn'
Cuts at: 3221 3734 3827 3221
Size: 513 93 5617
Fragments arranged by size:
5617 513 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2622 2643 2685 3000 3045 3174
Size: 399 229 603 21 42 315 45 129
Cuts at: 3174 3466 3542 4284 4369 5440 1391
Size: 292 76 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 603 399 315 292 229
129 85 76 45 42 21
BspEI T'CCGG_A
Cuts at: 5886 5886
Size: 6223
BspHI T'CATG_A
Cuts at: 2049 4112 5842 2049
Size: 2063 1730 2430
Fragments arranged by size:
2430 2063 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2215 2613 3578 844
Size: 1371 398 965 3489
Fragments arranged by size:
3489 1371 965 398
BsrBI CCG'CTC
Cuts at: 1394 1823 4110 1394
Size: 429 2287 3507
Fragments arranged by size:
3507 2287 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4718 4892 65
Size: 1183 3470 174 1396
Fragments arranged by size:
3470 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4867 5054 1700
Size: 110 3057 187 2869
Fragments arranged by size:
3057 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6223
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6223
BssSI C'ACGA_G
Cuts at: 2035 4283 5295 2035
Size: 2248 1012 2963
Fragments arranged by size:
2963 2248 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6223
BstBI TT'CG_AA
Cuts at: 5027 5027
Size: 6223
BstEII G'GTnAC_C
Cuts at: 1184 2227 2812 1184
Size: 1043 585 4595
Fragments arranged by size:
4595 1043 585
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2287
Size: 6223
BstYI r'GATC_y
Cuts at: 1972 3215 3553 3920 4305 4322 5774 5860 5872
Size: 1243 338 367 385 17 1452 86 12
Cuts at: 5872 6218 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1243 385 367 346 338 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3116 3166 3116
Size: 50 6173
BtgI C'CryG_G
Cuts at: 513 1065 2067 2955 513
Size: 552 1002 888 3781
Fragments arranged by size:
3781 1002 888 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5484
Fragments arranged by size:
5484 683 56
BtsI GCAGTG_nn'
Cuts at: 3739 4533 4553 3739
Size: 794 20 5409
Fragments arranged by size:
5409 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6223
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6188
DraI TTT'AAA
Cuts at: 3527 3527
Size: 6223
DraIII CAC_nnn'GTG
Cuts at: 2731 2731
Size: 6223
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3514 5230 809
Size: 507 2198 1716 1802
Fragments arranged by size:
2198 1802 1716 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2673 3021 3670
Size: 54 1425 50 275 18 843 348 649
Cuts at: 3670 4559 6188 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 843 649 348 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3670 1537
Size: 275 1858 4090
Fragments arranged by size:
4090 1858 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2369 2690 3094 4153 1353
Size: 1016 321 404 1059 3423
Fragments arranged by size:
3423 1059 1016 404 321
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2322 4810 5184 5330 387
Size: 1064 138 733 2488 374 146 1280
Fragments arranged by size:
2488 1280 1064 733 374 146 138
EcoICRI GAG'CTC
Cuts at: 2683 2998 3464 2683
Size: 315 466 5442
Fragments arranged by size:
5442 466 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2005 2204 3097 4286 4883 5670 5938
Size: 393 373 199 893 1189 597 787 268
Cuts at: 5938 1239
Size: 1524
Fragments arranged by size:
1524 1189 893 787 597 393 373 268
199
EcoNI CCTnn'n_nnAGG
Cuts at: 2141 2222 2141
Size: 81 6142
EcoRI G'AATT_C
Cuts at: 3532 3910 3532
Size: 378 5845
EcoRV GAT'ATC
Cuts at: 2191 2413 2191
Size: 222 6001
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2153 2153
Size: 6223
FspI TGC'GCA
Cuts at: 2863 2972 4729 2863
Size: 109 1757 4357
Fragments arranged by size:
4357 1757 109
FspAI rTGC'GCAy
Cuts at: 2972 2972
Size: 6223
HaeII r_GCGC'y
Cuts at: 1678 3439 5370 1678
Size: 1761 1931 2531
Fragments arranged by size:
2531 1931 1761
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3496 3528 4864 4896 4938
Size: 32 135 32 1924 32 1336 32 42
Cuts at: 4938 4970 1373
Size: 32 2626
Fragments arranged by size:
2626 1924 1336 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2521 2940 3567 3813 669
Size: 988 281 583 419 627 246 3079
Fragments arranged by size:
3079 988 627 583 419 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6223
HpaI GTT'AAC
Cuts at: 3813 3813
Size: 6223
KasI G'GCGC_C
Cuts at: 3435 3435
Size: 6223
KpnI G_GTAC'C
Cuts at: 3548 3548
Size: 6223
MfeI C'AATT_G
Cuts at: 2779 3822 2779
Size: 1043 5180
MluI A'CGCG_T
Cuts at: 6099 6099
Size: 6223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2466 5337 5521 1305
Size: 1161 2871 184 2007
Fragments arranged by size:
2871 2007 1161 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2675 3023 10
Size: 54 1425 343 843 348 3210
Fragments arranged by size:
3210 1425 843 348 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 4181 4540 4699 518
Size: 648 1119 1896 359 159 2042
Fragments arranged by size:
2042 1896 1119 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2418 3639 4316 5464 5709 1606
Size: 812 1221 677 1148 245 2120
Fragments arranged by size:
2120 1221 1148 812 677 245
NarI GG'CG_CC
Cuts at: 3436 3436
Size: 6223
NcoI C'CATG_G
Cuts at: 513 1065 2067 2955 513
Size: 552 1002 888 3781
Fragments arranged by size:
3781 1002 888 552
NdeI CA'TA_TG
Cuts at: 387 2095 387
Size: 1708 4515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6223
NotI GC'GGCC_GC
Cuts at: 3670 3670
Size: 6223
NspI r_CATG'y
Cuts at: 1132 1229 2548 3581 5126 1132
Size: 97 1319 1033 1545 2229
Fragments arranged by size:
2229 1545 1319 1033 97
PasI CC'CwG_GG
Cuts at: 1348 3045 3265 1348
Size: 1697 220 4306
Fragments arranged by size:
4306 1697 220
PciI A'CATG_T
Cuts at: 5122 5122
Size: 6223
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2163 2402 2628 2651 1108
Size: 1055 239 226 23 4680
Fragments arranged by size:
4680 1055 239 226 23
PmeI GTTT'AAAC
Cuts at: 3527 3527
Size: 6223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4292 4324 5805 5837 4292
Size: 32 1481 32 4678
Fragments arranged by size:
4678 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6223
PsiI TTA'TAA
Cuts at: 3793 3793
Size: 6223
PstI C_TGCA'G
Cuts at: 830 3575 830
Size: 2745 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6223
SacI G_AGCT'C
Cuts at: 2685 3000 3466 2685
Size: 315 466 5442
Fragments arranged by size:
5442 466 315
SalI G'TCGA_C
Cuts at: 1936 2938 3565 1936
Size: 1002 627 4594
Fragments arranged by size:
4594 1002 627
SapI GCTCTTCn'nnn_
Cuts at: 2369 2690 3094 2369
Size: 321 404 5498
Fragments arranged by size:
5498 404 321
SbfI CC_TGCA'GG
Cuts at: 3575 3575
Size: 6223
ScaI AGT'ACT
Cuts at: 3093 4471 6183 3093
Size: 1378 1712 3133
Fragments arranged by size:
3133 1712 1378
SfcI C'TryA_G
Cuts at: 826 1045 2748 3571 4706 5387 5578 826
Size: 219 1703 823 1135 681 191 1471
Fragments arranged by size:
1703 1471 1135 823 681 219 191
SfoI GGC'GCC
Cuts at: 3437 3437
Size: 6223
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6223
SmaI CCC'GGG
Cuts at: 1872 3550 1872
Size: 1678 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2756 3387 3465 4327 5228 5490
Size: 197 926 813 631 78 862 901 262
Cuts at: 5490 820
Size: 1553
Fragments arranged by size:
1553 926 901 862 813 631 262 197
78
SnaBI TAC'GTA
Cuts at: 493 4014 493
Size: 3521 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6223
SphI G_CATG'C
Cuts at: 1132 3581 1132
Size: 2449 3774
SspI AAT'ATT
Cuts at: 5 52 4147 5
Size: 47 4095 2081
Fragments arranged by size:
4095 2081 47
StuI AGG'CCT
Cuts at: 1517 2714 3478 1517
Size: 1197 764 4262
Fragments arranged by size:
4262 1197 764
StyI C'CwwG_G
Cuts at: 513 1065 2067 2116 2142 2157 2455 2955 3662
Size: 552 1002 49 26 15 298 500 707
Cuts at: 3662 513
Size: 3074
Fragments arranged by size:
3074 1002 707 552 500 298 49 26
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2170 4250 4267 4420 4605 5050 325
Size: 1255 590 2080 17 153 185 445 1498
Fragments arranged by size:
2080 1498 1255 590 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2535 3091 3383
Size: 274 80 33 51 1443 558 556 292
Cuts at: 3383 4469 6181 96
Size: 1086 1712 138
Fragments arranged by size:
1712 1443 1086 558 556 292 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6183 6215 6183
Size: 32 6191
Tth111I GACn'n_nGTC
Cuts at: 1316 3253 1316
Size: 1937 4286
XbaI T'CTAG_A
Cuts at: 3559 3559
Size: 6223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2160 2160
Size: 6223
XhoI C'TCGA_G
Cuts at: 1943 3387 1943
Size: 1444 4779
XmaI C'CCGG_G
Cuts at: 1870 3548 1870
Size: 1678 4545
XmnI GAAnn'nnTTC
Cuts at: 4352 4352
Size: 6223
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4031 276
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BfrBI BsiWI BsmBI
BstZ17I EcoO109I FseI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SrfI SwaI