Restriction map for
pFN21AE3534
(Circular) (Six-base) MAPSORT of: pFN21AE3534.seq Check: 7023 from: 1 to: 6628
pFN21AE3534 6628 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2506 2506
Size: 6628
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4438 278
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2213 3081 3971 1937
Size: 276 868 890 4594
Fragments arranged by size:
4594 890 868 276
Acc65I G'GTAC_C
Cuts at: 3949 3949
Size: 6628
AclI AA'CG_TT
Cuts at: 1563 2982 4755 5128 1563
Size: 1419 1773 373 3063
Fragments arranged by size:
3063 1773 1419 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3118 3641 4691 6075 6343 1632
Size: 1486 523 1050 1384 268 1917
Fragments arranged by size:
1917 1486 1384 1050 523 268
AfeI AGC'GCT
Cuts at: 2160 2160
Size: 6628
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6431
AflIII A'CryG_T
Cuts at: 1203 5527 6504 1203
Size: 4324 977 1327
Fragments arranged by size:
4324 1327 977
AgeI A'CCGG_T
Cuts at: 5459 5459
Size: 6628
AhdI GACnn_n'nnGTC
Cuts at: 3862 5357 3862
Size: 1495 5133
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6628
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3030 3798 1184
Size: 1846 768 4014
Fragments arranged by size:
4014 1846 768
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2759 2791 2759
Size: 32 6596
AlwNI CAG_nnn'CTG
Cuts at: 2176 4371 5943 2176
Size: 2195 1572 2861
Fragments arranged by size:
2861 2195 1572
ApaI G_GGCC'C
Cuts at: 2310 2494 3397 3625 2310
Size: 184 903 228 5313
Fragments arranged by size:
5313 903 228 184
ApaLI G'TGCA_C
Cuts at: 2452 4685 5841 2452
Size: 2233 1156 3239
Fragments arranged by size:
3239 2233 1156
ApoI r'AATT_y
Cuts at: 1493 3937 4163 4315 6424 1493
Size: 2444 226 152 2109 1697
Fragments arranged by size:
2444 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2363 2363
Size: 6628
AseI AT'TA_AT
Cuts at: 160 1032 5182 160
Size: 872 4150 1606
Fragments arranged by size:
4150 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
AvaI C'yCGr_G
Cuts at: 1870 1943 3090 3204 3369 3953 1870
Size: 73 1147 114 165 584 4545
Fragments arranged by size:
4545 1147 584 165 114 73
BamHI G'GATC_C
Cuts at: 3415 3958 4325 3415
Size: 543 367 5718
Fragments arranged by size:
5718 543 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2055 2371 2407 2664 3949
Size: 325 217 627 268 316 36 257 1285
Cuts at: 3949 5404 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1285 627 325 316 268 257
217 36
BanII G_rGCy'C
Cuts at: 1391 2016 2310 2494 3097 3397 3625 3947 1391
Size: 625 294 184 603 300 228 322 4072
Fragments arranged by size:
4072 625 603 322 300 294 228 184
BbeI G_GCGC'C
Cuts at: 2059 2668 2059
Size: 609 6019
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5657
BbvCI CC'TCA_GC
Cuts at: 2162 2162
Size: 6628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4819 4853 1903
Size: 34 2882 34 3678
Fragments arranged by size:
3678 2882 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2155 4520 5730 2155
Size: 2365 1210 3053
Fragments arranged by size:
3053 2365 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6628
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2038 5239 136
Size: 107 122 71 631 605 366 3201 1525
Fragments arranged by size:
3201 1525 631 605 366 122 107 71
BglII A'GATC_T
Cuts at: 6623 6623
Size: 6628
BlpI GC'TnA_GC
Cuts at: 4045 4045
Size: 6628
Bme1580I G_kGCm'C
Cuts at: 1790 2310 2376 2412 2456 2494 3397 3565 3625
Size: 520 66 36 44 38 903 168 60
Cuts at: 3625 4689 5845 1790
Size: 1064 1156 2573
Fragments arranged by size:
2573 1156 1064 903 520 168 66 60
44 38 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6628
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3619 4359 5317
Size: 416 53 83 399 24 2202 740 958
Cuts at: 5317 442
Size: 1753
Fragments arranged by size:
2202 1753 958 740 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2000 2342 3204 3621 5288 1239
Size: 761 342 862 417 1667 2579
Fragments arranged by size:
2579 1667 862 761 417 342
Bpu10I CC'TnA_GC
Cuts at: 2162 2162
Size: 6628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2125 4753 5618 5916 2125
Size: 2628 865 298 2837
Fragments arranged by size:
2837 2628 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2429 2816 5291 882
Size: 617 930 387 2475 2219
Fragments arranged by size:
2475 2219 930 617 387
BsaAI yAC'GTr
Cuts at: 493 1204 3270 4419 493
Size: 711 2066 1149 2702
Fragments arranged by size:
2702 2066 1149 711
BsaBI GATnn'nnATC
Cuts at: 6622 6622
Size: 6628
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2056 2504 2665 2793
Size: 53 83 186 936 523 448 161 128
Cuts at: 2793 4435 4817 5452 275
Size: 1642 382 635 1451
Fragments arranged by size:
1642 1451 936 635 523 448 382 186
161 128 83 53
BsaWI w'CCGG_w
Cuts at: 5061 5459 5733 5880 6291 5061
Size: 398 274 147 411 5398
Fragments arranged by size:
5398 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2759 2789 3594 3624 1178
Size: 30 165 30 1356 30 805 30 4182
Fragments arranged by size:
4182 1356 805 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2780 2888 1186
Size: 1594 108 4926
Fragments arranged by size:
4926 1594 108
BseYI C'CCAG_C
Cuts at: 2070 2591 3396 3591 3771 5831 6327 2070
Size: 521 805 195 180 2060 496 2371
Fragments arranged by size:
2371 2060 805 521 496 195 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2046 2792 3236 3344 1163
Size: 573 310 746 444 108 4447
Fragments arranged by size:
4447 746 573 444 310 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2051 2220 2265 2475 2842 4078
Size: 275 186 50 169 45 210 367 1236
Cuts at: 4078 4839 5867 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1236 1028 761 367 275 210 186
169 50 45
BsiHKAI G_wGCw'C
Cuts at: 2456 4689 4774 5845 2456
Size: 2233 85 1071 3239
Fragments arranged by size:
3239 2233 1071 85
BsmI GAATG_Cn'
Cuts at: 3233 3400 4139 4232 3233
Size: 167 739 93 5629
Fragments arranged by size:
5629 739 167 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2900 2900
Size: 6628
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2310 2376 2412 2456 2494 3097
Size: 399 226 294 66 36 44 38 603
Cuts at: 3097 3397 3565 3625 3947 4689 4774 5845 1391
Size: 300 168 60 322 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 603 399 322 300 294
226 168 85 66 60 44 38 36
BspEI T'CCGG_A
Cuts at: 6291 6291
Size: 6628
BspHI T'CATG_A
Cuts at: 4517 6247 4517
Size: 1730 4898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2506 3843 3983 844
Size: 1662 1337 140 3489
Fragments arranged by size:
3489 1662 1337 140
BsrBI CCG'CTC
Cuts at: 1394 1823 2609 2798 4515 1394
Size: 429 786 189 1717 3507
Fragments arranged by size:
3507 1717 786 429 189
BsrDI GCAATG_nn'
Cuts at: 65 1248 3339 5123 5297 65
Size: 1183 2091 1784 174 1396
Fragments arranged by size:
2091 1784 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2058 2419 2539 2598 2661 2879 3157
Size: 110 248 361 120 59 63 218 278
Cuts at: 3157 5272 5459 1700
Size: 2115 187 2869
Fragments arranged by size:
2869 2115 361 278 248 218 187 120
110 63 59
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6628
BssHII G'CGCG_C
Cuts at: 1925 2339 2363 2393 2844 1925
Size: 414 24 30 451 5709
Fragments arranged by size:
5709 451 414 30 24
BssSI C'ACGA_G
Cuts at: 4688 5700 4688
Size: 1012 5616
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2176 3172 794
Size: 1382 996 4250
Fragments arranged by size:
4250 1382 996
BstBI TT'CG_AA
Cuts at: 5432 5432
Size: 6628
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6628
BstXI CCAn_nnnn'nTGG
Cuts at: 3591 3757 3591
Size: 166 6462
BstYI r'GATC_y
Cuts at: 1972 3415 3958 4325 4710 4727 6179 6265 6277
Size: 1443 543 367 385 17 1452 86 12
Cuts at: 6277 6623 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1443 543 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3082 3082
Size: 6628
BtgI C'CryG_G
Cuts at: 513 1065 2004 3786 513
Size: 552 939 1782 3355
Fragments arranged by size:
3355 1782 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2617 487
Size: 683 56 1391 4498
Fragments arranged by size:
4498 1391 683 56
BtsI GCAGTG_nn'
Cuts at: 3327 4144 4938 4958 3327
Size: 817 794 20 4997
Fragments arranged by size:
4997 817 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6628
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6593
DraI TTT'AAA
Cuts at: 3932 3932
Size: 6628
DraIII CAC_nnn'GTG
Cuts at: 3569 3569
Size: 6628
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5635 809
Size: 507 4319 1802
Fragments arranged by size:
4319 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2048 2217 2262
Size: 54 1425 50 275 18 218 169 45
Cuts at: 2262 2296 2472 2571 2779 2839 3495 4075 4964
Size: 34 176 99 208 60 656 580 889
Cuts at: 4964 6593 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 656 580 275 218 208
176 169 99 60 54 50 45 43
34 18
EagI C'GGCC_G
Cuts at: 1537 1812 2048 2217 2262 2472 2839 4075 1537
Size: 275 236 169 45 210 367 1236 4090
Fragments arranged by size:
4090 1236 367 275 236 210 169 45
EarI CTCTTCn'nnn_
Cuts at: 1353 4558 1353
Size: 3205 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5215 5589 5735 387
Size: 1064 138 3626 374 146 1280
Fragments arranged by size:
3626 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2000 2342 3118 3204 3621 3641 4691
Size: 393 368 342 776 86 417 20 1050
Cuts at: 4691 5288 6075 6343 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1050 787 776 597 417 393 368
342 268 86 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2402 2873 2402
Size: 471 6157
EcoO109I rG'GnC_Cy
Cuts at: 2306 2490 2491 3393 3621 3832 2306
Size: 184 1 902 228 211 5102
Fragments arranged by size:
5102 902 228 211 184 1
EcoRI G'AATT_C
Cuts at: 3937 4315 3937
Size: 378 6250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3447 3447
Size: 6628
FspI TGC'GCA
Cuts at: 2759 5134 2759
Size: 2375 4253
HaeII r_GCGC'y
Cuts at: 1678 2059 2162 2668 5775 1678
Size: 381 103 506 3107 2531
Fragments arranged by size:
3107 2531 506 381 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3848 3880 5269 5301 5343
Size: 32 135 32 2276 32 1389 32 42
Cuts at: 5343 5375 1373
Size: 32 2626
Fragments arranged by size:
2626 2276 1389 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2214 3630 3972 4218 669
Size: 988 281 276 1416 342 246 3079
Fragments arranged by size:
3079 1416 988 342 281 276 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6628
HpaI GTT'AAC
Cuts at: 4218 4218
Size: 6628
KasI G'GCGC_C
Cuts at: 2055 2664 2055
Size: 609 6019
KpnI G_GTAC'C
Cuts at: 3953 3953
Size: 6628
MfeI C'AATT_G
Cuts at: 4227 4227
Size: 6628
MluI A'CGCG_T
Cuts at: 6504 6504
Size: 6628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3003 5742 5926 1305
Size: 1698 2739 184 2007
Fragments arranged by size:
2739 2007 1698 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4806
Fragments arranged by size:
4806 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3908 4586 4945 5104 518
Size: 648 2742 678 359 159 2042
Fragments arranged by size:
2742 2042 678 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2082 2131 2557 2738 3055 3862 4044 4721
Size: 476 49 426 181 317 807 182 677
Cuts at: 4721 5869 6114 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 807 677 476 426 317 245
182 181 49
NaeI GCC'GGC
Cuts at: 2060 2541 2600 2881 2060
Size: 481 59 281 5807
Fragments arranged by size:
5807 481 281 59
NarI GG'CG_CC
Cuts at: 2056 2665 2056
Size: 609 6019
NcoI C'CATG_G
Cuts at: 513 1065 2004 3786 513
Size: 552 939 1782 3355
Fragments arranged by size:
3355 1782 939 552
NdeI CA'TA_TG
Cuts at: 387 3651 387
Size: 3264 3364
NgoMIV G'CCGG_C
Cuts at: 2058 2539 2598 2879 2058
Size: 481 59 281 5807
Fragments arranged by size:
5807 481 281 59
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6628
NotI GC'GGCC_GC
Cuts at: 4075 4075
Size: 6628
NspI r_CATG'y
Cuts at: 1132 1229 3986 5531 1132
Size: 97 2757 1545 2229
Fragments arranged by size:
2757 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6628
PciI A'CATG_T
Cuts at: 5527 5527
Size: 6628
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2788 1108
Size: 1680 4948
PfoI T'CCnGG_A
Cuts at: 2111 2268 2111
Size: 157 6471
PmeI GTTT'AAAC
Cuts at: 3932 3932
Size: 6628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2759 2791 4697 4729 6210 6242 2759
Size: 32 1906 32 1481 32 3145
Fragments arranged by size:
3145 1906 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3832 3832
Size: 6628
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6628
PsiI TTA'TAA
Cuts at: 4198 4198
Size: 6628
PspOMI G'GGCC_C
Cuts at: 2306 2490 3393 3621 2306
Size: 184 903 228 5313
Fragments arranged by size:
5313 903 228 184
PstI C_TGCA'G
Cuts at: 830 2352 3054 3168 3980 830
Size: 1522 702 114 812 3478
Fragments arranged by size:
3478 1522 812 702 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6628
PvuII CAG'CTG
Cuts at: 2131 3055 3862 2131
Size: 924 807 4897
Fragments arranged by size:
4897 924 807
RsrII CG'GwC_CG
Cuts at: 2422 2422
Size: 6628
SalI G'TCGA_C
Cuts at: 1936 2212 3970 1936
Size: 276 1758 4594
Fragments arranged by size:
4594 1758 276
SbfI CC_TGCA'GG
Cuts at: 3980 3980
Size: 6628
ScaI AGT'ACT
Cuts at: 4876 6588 4876
Size: 1712 4916
SfcI C'TryA_G
Cuts at: 826 1045 2348 3050 3164 3976 5111 5792 5983
Size: 219 1303 702 114 812 1135 681 191
Cuts at: 5983 826
Size: 1471
Fragments arranged by size:
1471 1303 1135 812 702 681 219 191
114
SfoI GGC'GCC
Cuts at: 2057 2666 2057
Size: 609 6019
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
SgrAI Cr'CCGG_yG
Cuts at: 1700 2058 1700
Size: 358 6270
SmaI CCC'GGG
Cuts at: 1872 3955 1872
Size: 2083 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2104 4732 5633 5895 820
Size: 197 926 161 2628 901 262 1553
Fragments arranged by size:
2628 1553 926 901 262 197 161
SnaBI TAC'GTA
Cuts at: 493 4419 493
Size: 3926 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6628
SphI G_CATG'C
Cuts at: 1132 3986 1132
Size: 2854 3774
SspI AAT'ATT
Cuts at: 5 52 4552 5
Size: 47 4500 2081
Fragments arranged by size:
4500 2081 47
StuI AGG'CCT
Cuts at: 1517 2327 3041 1517
Size: 810 714 5104
Fragments arranged by size:
5104 810 714
StyI C'CwwG_G
Cuts at: 513 1065 2004 3786 3867 4067 513
Size: 552 939 1782 81 200 3074
Fragments arranged by size:
3074 1782 939 552 200 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2077 3671 4655 4672 4825 5010 5455
Size: 1255 497 1594 984 17 153 185 445
Cuts at: 5455 325
Size: 1498
Fragments arranged by size:
1594 1498 1255 984 497 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4874 6586 96
Size: 274 80 33 51 1443 2897 1712 138
Fragments arranged by size:
2897 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6588 6620 6588
Size: 32 6596
Tth111I GACn'n_nGTC
Cuts at: 1316 2356 2503 1316
Size: 1040 147 5441
Fragments arranged by size:
5441 1040 147
XbaI T'CTAG_A
Cuts at: 3964 3964
Size: 6628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3313 3313
Size: 6628
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6628
XmaI C'CCGG_G
Cuts at: 1870 3953 1870
Size: 2083 4545
XmnI GAAnn'nnTTC
Cuts at: 4757 4757
Size: 6628
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4436 276
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BfrBI BplI BsiWI Bsu36I EcoICRI EcoRV
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI