Restriction map for
pFN21AE3529
(Circular) (Six-base) MAPSORT of: pFN21AE3529.seq Check: 9277 from: 1 to: 5392
pFN21AE3529 5392 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3202 278
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2351 2735 1937
Size: 414 384 4594
Fragments arranged by size:
4594 414 384
Acc65I G'GTAC_C
Cuts at: 2495 2713 2495
Size: 218 5174
AclI AA'CG_TT
Cuts at: 1563 3519 3892 1563
Size: 1956 373 3063
Fragments arranged by size:
3063 1956 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2584 3455 4839 5107 1632
Size: 952 871 1384 268 1917
Fragments arranged by size:
1917 1384 952 871 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5195
AflIII A'CryG_T
Cuts at: 1203 4291 5268 1203
Size: 3088 977 1327
Fragments arranged by size:
3088 1327 977
AgeI A'CCGG_T
Cuts at: 4223 4223
Size: 5392
AhdI GACnn_n'nnGTC
Cuts at: 4121 4121
Size: 5392
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5392
AlwNI CAG_nnn'CTG
Cuts at: 2155 3135 4707 2155
Size: 980 1572 2840
Fragments arranged by size:
2840 1572 980
ApaLI G'TGCA_C
Cuts at: 3449 4605 3449
Size: 1156 4236
ApoI r'AATT_y
Cuts at: 1493 2701 2927 3079 5188 1493
Size: 1208 226 152 2109 1697
Fragments arranged by size:
2109 1697 1208 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3946 160
Size: 872 2914 1606
Fragments arranged by size:
2914 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
AvaI C'yCGr_G
Cuts at: 1870 1943 2591 2717 1870
Size: 73 648 126 4545
Fragments arranged by size:
4545 648 126 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2487 2520 2487
Size: 33 5359
BamHI G'GATC_C
Cuts at: 2722 3089 2722
Size: 367 5025
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2495 2713 4168 618
Size: 325 217 627 708 218 1455 1842
Fragments arranged by size:
1842 1455 708 627 325 218 217
BanII G_rGCy'C
Cuts at: 1391 2592 2711 1391
Size: 1201 119 4072
Fragments arranged by size:
4072 1201 119
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2019 928
Size: 971 120 4301
Fragments arranged by size:
4301 971 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2635 2669 3583 3617 1903
Size: 34 698 34 914 34 3678
Fragments arranged by size:
3678 914 698 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3284 4494 3284
Size: 1210 4182
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5392
BfrBI ATG'CAT
Cuts at: 2263 2388 2263
Size: 125 5267
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4003 136
Size: 107 122 71 631 605 2331 1525
Fragments arranged by size:
2331 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5387 5387
Size: 5392
BlpI GC'TnA_GC
Cuts at: 2809 2809
Size: 5392
Bme1580I G_kGCm'C
Cuts at: 1790 3453 4609 1790
Size: 1663 1156 2573
Fragments arranged by size:
2573 1663 1156
BmgBI CAC'GTC
Cuts at: 1318 2626 1318
Size: 1308 4084
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3123 4081 442
Size: 416 53 83 399 24 1706 958 1753
Fragments arranged by size:
1753 1706 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5392
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2695 2695
Size: 5392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4052 1239
Size: 2813 2579
Bpu10I CC'TnA_GC
Cuts at: 2339 2339
Size: 5392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3517 4382 4680 3517
Size: 865 298 4229
Fragments arranged by size:
4229 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2651 4055 882
Size: 617 1152 1404 2219
Fragments arranged by size:
2219 1404 1152 617
BsaAI yAC'GTr
Cuts at: 493 1204 2163 3183 493
Size: 711 959 1020 2702
Fragments arranged by size:
2702 1020 959 711
BsaBI GATnn'nnATC
Cuts at: 5386 5386
Size: 5392
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3199 3581 4216 275
Size: 53 83 186 936 1666 382 635 1451
Fragments arranged by size:
1666 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3825 4223 4497 4644 5055 3825
Size: 398 274 147 411 4162
Fragments arranged by size:
4162 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5167
Fragments arranged by size:
5167 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2633 2684 1186
Size: 1447 51 3894
Fragments arranged by size:
3894 1447 51
BseYI C'CCAG_C
Cuts at: 4595 5091 4595
Size: 496 4896
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4819
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2842 3603 4631 1540
Size: 275 186 841 761 1028 2301
Fragments arranged by size:
2301 1028 841 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2345 2592 3453 3538 4609 2345
Size: 247 861 85 1071 3128
Fragments arranged by size:
3128 1071 861 247 85
BsiWI C'GTAC_G
Cuts at: 2163 2163
Size: 5392
BsmI GAATG_Cn'
Cuts at: 2903 2996 2903
Size: 93 5299
BsmBI CGTCTCn'nnnn_
Cuts at: 2485 2485
Size: 5392
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2345 2592 2711 3453 3538 4609 1391
Size: 399 555 247 119 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 555 399 247 119 85
BspEI T'CCGG_A
Cuts at: 5055 5055
Size: 5392
BspHI T'CATG_A
Cuts at: 3281 5011 3281
Size: 1730 3662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2747 844
Size: 1903 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2248 3279 1394
Size: 429 425 1031 3507
Fragments arranged by size:
3507 1031 429 425
BsrDI GCAATG_nn'
Cuts at: 65 1248 3887 4061 65
Size: 1183 2639 174 1396
Fragments arranged by size:
2639 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4036 4223 1700
Size: 110 2226 187 2869
Fragments arranged by size:
2869 2226 187 110
BsrGI T'GTAC_A
Cuts at: 96 2178 2311 96
Size: 2082 133 3177
Fragments arranged by size:
3177 2082 133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5392
BssSI C'ACGA_G
Cuts at: 3452 4464 3452
Size: 1012 4380
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5392
BstBI TT'CG_AA
Cuts at: 4196 4196
Size: 5392
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5392
BstXI CCAn_nnnn'nTGG
Cuts at: 2116 2218 2116
Size: 102 5290
BstYI r'GATC_y
Cuts at: 1972 2379 2722 3089 3474 3491 4943 5029 5041
Size: 407 343 367 385 17 1452 86 12
Cuts at: 5041 5387 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 407 385 367 346 343 86
17 12
BstZ17I GTA'TAC
Cuts at: 2352 2352
Size: 5392
BtgI C'CryG_G
Cuts at: 513 1065 2004 2193 513
Size: 552 939 189 3712
Fragments arranged by size:
3712 939 552 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4653
Fragments arranged by size:
4653 683 56
BtsI GCAGTG_nn'
Cuts at: 2103 2908 3702 3722 2103
Size: 805 794 20 3773
Fragments arranged by size:
3773 805 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5357
DraI TTT'AAA
Cuts at: 2696 2696
Size: 5392
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4399 809
Size: 507 3083 1802
Fragments arranged by size:
3083 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2397 2839 3728
Size: 54 1425 50 275 18 567 442 889
Cuts at: 3728 5357 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 567 442 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2839 1537
Size: 275 1027 4090
Fragments arranged by size:
4090 1027 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2519 2610 3322 1353
Size: 1166 91 712 3423
Fragments arranged by size:
3423 1166 712 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3979 4353 4499 387
Size: 1064 138 2390 374 146 1280
Fragments arranged by size:
2390 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2590 2590
Size: 5392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2584 3455 4052 4839 5107 1239
Size: 393 952 871 597 787 268 1524
Fragments arranged by size:
1524 952 871 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2701 3079 2701
Size: 378 5014
FspI TGC'GCA
Cuts at: 3898 3898
Size: 5392
HaeII r_GCGC'y
Cuts at: 1678 4539 1678
Size: 2861 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2648 2680 4033 4065 4107
Size: 32 135 32 1076 32 1353 32 42
Cuts at: 4107 4139 1373
Size: 32 2626
Fragments arranged by size:
2626 1353 1076 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2736 2982 669
Size: 988 281 798 246 3079
Fragments arranged by size:
3079 988 798 281 246
HindIII A'AGCT_T
Cuts at: 748 2437 748
Size: 1689 3703
HpaI GTT'AAC
Cuts at: 2982 2982
Size: 5392
KpnI G_GTAC'C
Cuts at: 2499 2717 2499
Size: 218 5174
MfeI C'AATT_G
Cuts at: 2991 2991
Size: 5392
MluI A'CGCG_T
Cuts at: 5268 5268
Size: 5392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2016 4506 4690 1305
Size: 711 2490 184 2007
Fragments arranged by size:
2490 2007 711 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2399 10
Size: 54 1425 343 567 3003
Fragments arranged by size:
3003 1425 567 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 2300 2393 2423 3350 3709 3868
Size: 648 1050 84 93 30 927 359 159
Cuts at: 3868 518
Size: 2042
Fragments arranged by size:
2042 1050 927 648 359 159 93 84
30
MspA1I CmG'CkG
Cuts at: 1606 2155 2808 3485 4633 4878 1606
Size: 549 653 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 653 549 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3901
Fragments arranged by size:
3901 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5392
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5392
NotI GC'GGCC_GC
Cuts at: 2839 2839
Size: 5392
NsiI A_TGCA'T
Cuts at: 2265 2390 2265
Size: 125 5267
NspI r_CATG'y
Cuts at: 1132 1229 2263 2267 2750 4295 1132
Size: 97 1034 4 483 1545 2229
Fragments arranged by size:
2229 1545 1034 483 97 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5392
PciI A'CATG_T
Cuts at: 4291 4291
Size: 5392
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5392
PmeI GTTT'AAAC
Cuts at: 2696 2696
Size: 5392
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3461 3493 4974 5006 3461
Size: 32 1481 32 3847
Fragments arranged by size:
3847 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5392
PsiI TTA'TAA
Cuts at: 2962 2962
Size: 5392
PstI C_TGCA'G
Cuts at: 830 2744 830
Size: 1914 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5392
PvuII CAG'CTG
Cuts at: 2155 2155
Size: 5392
SacI G_AGCT'C
Cuts at: 2592 2592
Size: 5392
SalI G'TCGA_C
Cuts at: 1936 2734 1936
Size: 798 4594
SapI GCTCTTCn'nnn_
Cuts at: 2519 2519
Size: 5392
SbfI CC_TGCA'GG
Cuts at: 2744 2744
Size: 5392
ScaI AGT'ACT
Cuts at: 3640 5352 3640
Size: 1712 3680
SexAI A'CCwGG_T
Cuts at: 2345 2345
Size: 5392
SfcI C'TryA_G
Cuts at: 826 1045 2329 2740 3875 4556 4747 826
Size: 219 1284 411 1135 681 191 1471
Fragments arranged by size:
1471 1284 1135 681 411 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5392
SmaI CCC'GGG
Cuts at: 1872 2719 1872
Size: 847 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2591 3496 4397 4659 820
Size: 197 926 648 905 901 262 1553
Fragments arranged by size:
1553 926 905 901 648 262 197
SnaBI TAC'GTA
Cuts at: 493 3183 493
Size: 2690 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5392
SphI G_CATG'C
Cuts at: 1132 2263 2267 2750 1132
Size: 1131 4 483 3774
Fragments arranged by size:
3774 1131 483 4
SspI AAT'ATT
Cuts at: 5 52 3316 5
Size: 47 3264 2081
Fragments arranged by size:
3264 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5392
StyI C'CwwG_G
Cuts at: 513 1065 2004 2041 2831 513
Size: 552 939 37 790 3074
Fragments arranged by size:
3074 939 790 552 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3419 3436 3589 3774 4219 325
Size: 1255 1839 17 153 185 445 1498
Fragments arranged by size:
1839 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 2311 3638
Size: 274 80 33 51 1443 201 133 1327
Cuts at: 3638 5350 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1327 274 201 138 133 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5352 5384 5352
Size: 32 5360
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5392
XbaI T'CTAG_A
Cuts at: 2728 2728
Size: 5392
XhoI C'TCGA_G
Cuts at: 1943 2591 1943
Size: 648 4744
XmaI C'CCGG_G
Cuts at: 1870 2717 1870
Size: 847 4545
XmnI GAAnn'nnTTC
Cuts at: 2513 3521 2513
Size: 1008 4384
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3200 276
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI
SfoI SrfI SwaI XcmI