Restriction map for
pFN21AE3528
(Circular) (Six-base) MAPSORT of: pFN21AE3528.seq Check: 3971 from: 1 to: 7441
pFN21AE3528 7441 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2226 2226
Size: 7441
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5251 278
Size: 53 83 186 936 3715 2468
Fragments arranged by size:
3715 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4316 4784 1937
Size: 2379 468 4594
Fragments arranged by size:
4594 2379 468
Acc65I G'GTAC_C
Cuts at: 4762 4762
Size: 7441
AclI AA'CG_TT
Cuts at: 1563 5568 5941 1563
Size: 4005 373 3063
Fragments arranged by size:
4005 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2402 2576 4061 4229 5504 6888 7156 1632
Size: 770 174 1485 168 1275 1384 268 1917
Fragments arranged by size:
1917 1485 1384 1275 770 268 174 168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7244
AflIII A'CryG_T
Cuts at: 1203 2241 2470 6340 7317 1203
Size: 1038 229 3870 977 1327
Fragments arranged by size:
3870 1327 1038 977 229
AgeI A'CCGG_T
Cuts at: 6272 6272
Size: 7441
AhdI GACnn_n'nnGTC
Cuts at: 6170 6170
Size: 7441
AleI CACnn'nnGTG
Cuts at: 1166 3257 1166
Size: 2091 5350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2393 3135 1184
Size: 1209 742 5490
Fragments arranged by size:
5490 1209 742
AlwNI CAG_nnn'CTG
Cuts at: 2851 3947 5184 6756 2851
Size: 1096 1237 1572 3536
Fragments arranged by size:
3536 1572 1237 1096
ApaLI G'TGCA_C
Cuts at: 3666 5498 6654 3666
Size: 1832 1156 4453
Fragments arranged by size:
4453 1832 1156
ApoI r'AATT_y
Cuts at: 1493 2037 2141 2151 3217 3415 4238 4513 4750
Size: 544 104 10 1066 198 823 275 237
Cuts at: 4750 4976 5128 7237 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1066 823 544 275 237 226
198 152 104 10
AseI AT'TA_AT
Cuts at: 160 1032 5995 160
Size: 872 4963 1606
Fragments arranged by size:
4963 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7441
AvaI C'yCGr_G
Cuts at: 1870 1943 2675 4766 1870
Size: 73 732 2091 4545
Fragments arranged by size:
4545 2091 732 73
BamHI G'GATC_C
Cuts at: 4456 4771 5138 4456
Size: 315 367 6759
Fragments arranged by size:
6759 367 315
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2824 4057 4252 4762 6217
Size: 325 217 627 1037 1233 195 510 1455
Cuts at: 6217 618
Size: 1842
Fragments arranged by size:
1842 1455 1233 1037 627 510 325 217
195
BanII G_rGCy'C
Cuts at: 1391 4508 4760 1391
Size: 3117 252 4072
Fragments arranged by size:
4072 3117 252
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2057 3168 928
Size: 971 158 1111 5201
Fragments arranged by size:
5201 1111 971 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5632 5666 1903
Size: 34 3695 34 3678
Fragments arranged by size:
3695 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5333 6543 5333
Size: 1210 6231
BclI T'GATC_A
Cuts at: 1548 4011 1548
Size: 2463 4978
BfrBI ATG'CAT
Cuts at: 3235 3235
Size: 7441
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6052 136
Size: 107 122 71 631 605 4380 1525
Fragments arranged by size:
4380 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3424 7436 3424
Size: 4012 3429
BlpI GC'TnA_GC
Cuts at: 2162 4858 2162
Size: 2696 4745
Bme1580I G_kGCm'C
Cuts at: 1790 3670 4363 5502 6658 1790
Size: 1880 693 1139 1156 2573
Fragments arranged by size:
2573 1880 1156 1139 693
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7441
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2064 3204 4373
Size: 416 53 83 399 24 647 1140 1169
Cuts at: 4373 5172 6130 442
Size: 799 958 1753
Fragments arranged by size:
1753 1169 1140 958 799 647 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3499 4190 4245 4448 6101 1239
Size: 2260 691 55 203 1653 2579
Fragments arranged by size:
2579 2260 1653 691 203 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2934 3326 3676 4170 5566 6431 6729 2934
Size: 392 350 494 1396 865 298 3646
Fragments arranged by size:
3646 1396 865 494 392 350 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3858 4295 4595 6104 882
Size: 617 2359 437 300 1509 2219
Fragments arranged by size:
2359 2219 1509 617 437 300
BsaAI yAC'GTr
Cuts at: 493 1204 5232 493
Size: 711 4028 2702
Fragments arranged by size:
4028 2702 711
BsaBI GATnn'nnATC
Cuts at: 7435 7435
Size: 7441
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5248 5630 6265 275
Size: 53 83 186 936 3715 382 635 1451
Fragments arranged by size:
3715 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5874 6272 6546 6693 7104 5874
Size: 398 274 147 411 6211
Fragments arranged by size:
6211 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7216
Fragments arranged by size:
7216 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4235 4685 1186
Size: 3049 450 3942
Fragments arranged by size:
3942 3049 450
BseYI C'CCAG_C
Cuts at: 3003 6644 7140 3003
Size: 3641 496 3304
Fragments arranged by size:
3641 3304 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3362 3867 1163
Size: 573 1626 505 4737
Fragments arranged by size:
4737 1626 573 505
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4891 5652 6680 1540
Size: 275 186 2890 761 1028 2301
Fragments arranged by size:
2890 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2790 3670 4508 5502 5587 6658 2790
Size: 880 838 994 85 1071 3573
Fragments arranged by size:
3573 1071 994 880 838 85
BsmI GAATG_Cn'
Cuts at: 2899 3808 4952 5045 2899
Size: 909 1144 93 5295
Fragments arranged by size:
5295 1144 909 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2790 3670 4363 4508 4760 5502 5587
Size: 399 1000 880 693 145 252 742 85
Cuts at: 5587 6658 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1000 880 742 693 399 252
145 85
BspEI T'CCGG_A
Cuts at: 7104 7104
Size: 7441
BspHI T'CATG_A
Cuts at: 3124 5330 7060 3124
Size: 2206 1730 3505
Fragments arranged by size:
3505 2206 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2226 3384 4796 844
Size: 1382 1158 1412 3489
Fragments arranged by size:
3489 1412 1382 1158
BsrBI CCG'CTC
Cuts at: 1394 1823 5328 1394
Size: 429 3505 3507
Fragments arranged by size:
3507 3505 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3634 5936 6110 65
Size: 1183 2386 2302 174 1396
Fragments arranged by size:
2386 2302 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6085 6272 1700
Size: 110 4275 187 2869
Fragments arranged by size:
4275 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2533 96
Size: 2437 5004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7441
BssSI C'ACGA_G
Cuts at: 5501 6513 5501
Size: 1012 6429
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7441
BstBI TT'CG_AA
Cuts at: 6245 6245
Size: 7441
BstEII G'GTnAC_C
Cuts at: 1184 2798 1184
Size: 1614 5827
BstXI CCAn_nnnn'nTGG
Cuts at: 2054 2054
Size: 7441
BstYI r'GATC_y
Cuts at: 1972 3424 4181 4456 4771 5138 5523 5540 6992
Size: 1452 757 275 315 367 385 17 1452
Cuts at: 6992 7078 7090 7436 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1452 757 385 367 346 315
275 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4731 4731
Size: 7441
BtgI C'CryG_G
Cuts at: 513 1065 2960 513
Size: 552 1895 4994
Fragments arranged by size:
4994 1895 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4548 487
Size: 683 56 3322 3380
Fragments arranged by size:
3380 3322 683 56
BtsI GCAGTG_nn'
Cuts at: 4217 4957 5751 5771 4217
Size: 740 794 20 5887
Fragments arranged by size:
5887 794 740 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7441
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3384 3419 529
Size: 35 2820 35 4551
Fragments arranged by size:
4551 2820 35 35
DraI TTT'AAA
Cuts at: 3436 4745 3436
Size: 1309 6132
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6448 809
Size: 507 5132 1802
Fragments arranged by size:
5132 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4888 5777 7406
Size: 54 1425 50 275 18 3058 889 1629
Cuts at: 7406 8
Size: 43
Fragments arranged by size:
3058 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4888 1537
Size: 275 3076 4090
Fragments arranged by size:
4090 3076 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3438 3473 3654 3984 4293 5371 1353
Size: 2085 35 181 330 309 1078 3423
Fragments arranged by size:
3423 2085 1078 330 309 181 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6028 6402 6548 387
Size: 1064 138 4439 374 146 1280
Fragments arranged by size:
4439 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4506 4506
Size: 7441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2402 2576 3499 4061 4190 4229 4245
Size: 393 770 174 923 562 129 39 16
Cuts at: 4245 4448 5504 6101 6888 7156 1239
Size: 203 1056 597 787 268 1524
Fragments arranged by size:
1524 1056 923 787 770 597 562 393
268 203 174 129 39 16
EcoO109I rG'GnC_Cy
Cuts at: 3520 3520
Size: 7441
EcoRI G'AATT_C
Cuts at: 3415 4750 5128 3415
Size: 1335 378 5728
Fragments arranged by size:
5728 1335 378
EcoRV GAT'ATC
Cuts at: 2010 2010
Size: 7441
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3464 3633 3464
Size: 169 7272
FspI TGC'GCA
Cuts at: 5947 5947
Size: 7441
HaeII r_GCGC'y
Cuts at: 1678 6588 1678
Size: 4910 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 4294 4326 4441
Size: 32 135 32 677 32 2013 32 115
Cuts at: 4441 4473 6082 6114 6156 6188 1373
Size: 32 1609 32 42 32 2626
Fragments arranged by size:
2626 2013 1609 677 135 115 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4785 5031 669
Size: 988 281 2847 246 3079
Fragments arranged by size:
3079 2847 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3321 748
Size: 2573 4868
HpaI GTT'AAC
Cuts at: 5031 5031
Size: 7441
KpnI G_GTAC'C
Cuts at: 4766 4766
Size: 7441
MfeI C'AATT_G
Cuts at: 5040 5040
Size: 7441
MluI A'CGCG_T
Cuts at: 7317 7317
Size: 7441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6555 6739 1305
Size: 5250 184 2007
Fragments arranged by size:
5250 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5619
Fragments arranged by size:
5619 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3257 5399 5758 5917 518
Size: 648 2091 2142 359 159 2042
Fragments arranged by size:
2142 2091 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2161 3007 3154 4857 5534 6682 6927 1606
Size: 555 846 147 1703 677 1148 245 2120
Fragments arranged by size:
2120 1703 1148 846 677 555 245 147
NcoI C'CATG_G
Cuts at: 513 1065 2960 513
Size: 552 1895 4994
Fragments arranged by size:
4994 1895 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7441
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7441
NotI GC'GGCC_GC
Cuts at: 4888 4888
Size: 7441
NsiI A_TGCA'T
Cuts at: 3237 3237
Size: 7441
NspI r_CATG'y
Cuts at: 1132 1229 2474 3112 3239 4654 4799 6344 1132
Size: 97 1245 638 127 1415 145 1545 2229
Fragments arranged by size:
2229 1545 1415 1245 638 145 127 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7441
PciI A'CATG_T
Cuts at: 2470 6340 2470
Size: 3870 3571
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7441
PfoI T'CCnGG_A
Cuts at: 2298 3690 2298
Size: 1392 6049
PmeI GTTT'AAAC
Cuts at: 4745 4745
Size: 7441
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5510 5542 7023 7055 5510
Size: 32 1481 32 5896
Fragments arranged by size:
5896 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3520 3520
Size: 7441
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7441
PsiI TTA'TAA
Cuts at: 2147 5011 2147
Size: 2864 4577
PstI C_TGCA'G
Cuts at: 830 2856 4793 830
Size: 2026 1937 3478
Fragments arranged by size:
3478 2026 1937
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7441
PvuII CAG'CTG
Cuts at: 2161 3007 3154 2161
Size: 846 147 6448
Fragments arranged by size:
6448 846 147
SacI G_AGCT'C
Cuts at: 4508 4508
Size: 7441
SalI G'TCGA_C
Cuts at: 1936 4783 1936
Size: 2847 4594
SapI GCTCTTCn'nnn_
Cuts at: 3438 3438
Size: 7441
SbfI CC_TGCA'GG
Cuts at: 4793 4793
Size: 7441
ScaI AGT'ACT
Cuts at: 5689 7401 5689
Size: 1712 5729
SfcI C'TryA_G
Cuts at: 826 1045 2269 2852 3028 3544 4317 4471 4789
Size: 219 1224 583 176 516 773 154 318
Cuts at: 4789 5924 6605 6796 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1224 1135 773 681 583 516 318
219 191 176 154
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7441
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7441
SmaI CCC'GGG
Cuts at: 1872 4768 1872
Size: 2896 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2949 3341 3655 4185 5545 6446
Size: 197 926 1006 392 314 530 1360 901
Cuts at: 6446 6708 820
Size: 262 1553
Fragments arranged by size:
1553 1360 1006 926 901 530 392 314
262 197
SnaBI TAC'GTA
Cuts at: 493 5232 493
Size: 4739 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7441
SphI G_CATG'C
Cuts at: 1132 4799 1132
Size: 3667 3774
SspI AAT'ATT
Cuts at: 5 52 3096 5365 5
Size: 47 3044 2269 2081
Fragments arranged by size:
3044 2269 2081 47
StuI AGG'CCT
Cuts at: 1517 4469 4559 1517
Size: 2952 90 4399
Fragments arranged by size:
4399 2952 90
StyI C'CwwG_G
Cuts at: 513 1065 2960 4354 4425 4880 513
Size: 552 1895 1394 71 455 3074
Fragments arranged by size:
3074 1895 1394 552 455 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5468 5485 5638 5823 6268 325
Size: 1255 3888 17 153 185 445 1498
Fragments arranged by size:
3888 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2533 3032 5687
Size: 274 80 33 51 1443 556 499 2655
Cuts at: 5687 7399 96
Size: 1712 138
Fragments arranged by size:
2655 1712 1443 556 499 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3190 3222 7401 7433 3190
Size: 32 4179 32 3198
Fragments arranged by size:
4179 3198 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2211 4124 1316
Size: 895 1913 4633
Fragments arranged by size:
4633 1913 895
XbaI T'CTAG_A
Cuts at: 4777 4777
Size: 7441
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3772 3830 3772
Size: 58 7383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7441
XmaI C'CCGG_G
Cuts at: 1870 4766 1870
Size: 2896 4545
XmnI GAAnn'nnTTC
Cuts at: 2294 2970 3023 4242 5570 2294
Size: 676 53 1219 1328 4165
Fragments arranged by size:
4165 1328 1219 676 53
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5249 276
Size: 53 83 186 936 3715 2468
Fragments arranged by size:
3715 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI