Restriction map for
pFN21AE3527
(Circular) (Six-base) MAPSORT of: pFN21AE3527.seq Check: 3625 from: 1 to: 5992
pFN21AE3527 5992 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3802 278
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3335 1937
Size: 1398 4594
Acc65I G'GTAC_C
Cuts at: 3313 3313
Size: 5992
AclI AA'CG_TT
Cuts at: 1563 4119 4492 1563
Size: 2556 373 3063
Fragments arranged by size:
3063 2556 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4055 5439 5707 1632
Size: 2423 1384 268 1917
Fragments arranged by size:
2423 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5795
AflIII A'CryG_T
Cuts at: 1203 2516 4891 5868 1203
Size: 1313 2375 977 1327
Fragments arranged by size:
2375 1327 1313 977
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 4721 4721
Size: 5992
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2514 1184
Size: 1330 4662
AlwNI CAG_nnn'CTG
Cuts at: 2132 3735 5307 2132
Size: 1603 1572 2817
Fragments arranged by size:
2817 1603 1572
ApaLI G'TGCA_C
Cuts at: 4049 5205 4049
Size: 1156 4836
ApoI r'AATT_y
Cuts at: 1493 3301 3527 3679 5788 1493
Size: 1808 226 152 2109 1697
Fragments arranged by size:
2109 1808 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4546 160
Size: 872 3514 1606
Fragments arranged by size:
3514 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2084 2615 3317 1870
Size: 73 73 68 531 702 4545
Fragments arranged by size:
4545 702 531 73 73 68
BamHI G'GATC_C
Cuts at: 3028 3322 3689 3028
Size: 294 367 5331
Fragments arranged by size:
5331 367 294
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 2238 2485 3138 3313
Size: 325 217 627 293 158 247 653 175
Cuts at: 3313 4768 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 653 627 325 293 247 217
175 158
BanII G_rGCy'C
Cuts at: 1391 2430 2721 3311 1391
Size: 1039 291 590 4072
Fragments arranged by size:
4072 1039 590 291
BbeI G_GCGC'C
Cuts at: 2084 2242 2489 2084
Size: 158 247 5587
Fragments arranged by size:
5587 247 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2519 3100 928
Size: 971 620 581 3820
Fragments arranged by size:
3820 971 620 581
BbvCI CC'TCA_GC
Cuts at: 3279 3279
Size: 5992
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2158 2192 2383 2417 3057 3091 4183
Size: 34 221 34 191 34 640 34 1092
Cuts at: 4183 4217 1903
Size: 34 3678
Fragments arranged by size:
3678 1092 640 221 191 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3884 5094 3884
Size: 1210 4782
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5992
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2483 4603 136
Size: 107 122 71 631 605 811 2120 1525
Fragments arranged by size:
2120 1525 811 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3021 5987 3021
Size: 2966 3026
BlpI GC'TnA_GC
Cuts at: 3409 3409
Size: 5992
Bme1580I G_kGCm'C
Cuts at: 1790 2818 4053 5209 1790
Size: 1028 1235 1156 2573
Fragments arranged by size:
2573 1235 1156 1028
BmgBI CAC'GTC
Cuts at: 1318 2622 1318
Size: 1304 4688
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2686 2812 3723
Size: 416 53 83 399 24 1269 126 911
Cuts at: 3723 4681 442
Size: 958 1753
Fragments arranged by size:
1753 1269 958 911 416 399 126 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5992
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3187 3187
Size: 5992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2109 3133 3207 4652 1239
Size: 870 1024 74 1445 2579
Fragments arranged by size:
2579 1445 1024 870 74
Bpu10I CC'TnA_GC
Cuts at: 3279 3279
Size: 5992
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4117 4982 5280 4117
Size: 865 298 4829
Fragments arranged by size:
4829 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2006 2895 4655 882
Size: 617 507 889 1760 2219
Fragments arranged by size:
2219 1760 889 617 507
BsaAI yAC'GTr
Cuts at: 493 1204 3783 493
Size: 711 2579 2702
Fragments arranged by size:
2702 2579 711
BsaBI GATnn'nnATC
Cuts at: 5986 5986
Size: 5992
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2081 2239 2486 3799
Size: 53 83 186 936 548 158 247 1313
Cuts at: 3799 4181 4816 275
Size: 382 635 1451
Fragments arranged by size:
1451 1313 936 635 548 382 247 186
158 83 53
BsaWI w'CCGG_w
Cuts at: 4425 4823 5097 5244 5655 4425
Size: 398 274 147 411 4762
Fragments arranged by size:
4762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2107 2137 1178
Size: 30 165 30 704 30 5033
Fragments arranged by size:
5033 704 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2422 3219 1186
Size: 1236 797 3959
Fragments arranged by size:
3959 1236 797
BseYI C'CCAG_C
Cuts at: 2584 5195 5691 2584
Size: 2611 496 2885
Fragments arranged by size:
2885 2611 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2029 2377 3212 1163
Size: 573 293 348 835 3943
Fragments arranged by size:
3943 835 573 348 293
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2331 2838 3442 4203 5231 1540
Size: 275 186 330 507 604 761 1028 2301
Fragments arranged by size:
2301 1028 761 604 507 330 275 186
BsiHKAI G_wGCw'C
Cuts at: 2370 2562 2946 4053 4138 5209 2370
Size: 192 384 1107 85 1071 3153
Fragments arranged by size:
3153 1107 1071 384 192 85
BsmI GAATG_Cn'
Cuts at: 2981 3503 3596 2981
Size: 522 93 5377
Fragments arranged by size:
5377 522 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2834 2834
Size: 5992
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2370 2430 2562 2721 2818 2946 3311
Size: 399 580 60 132 159 97 128 365
Cuts at: 3311 4053 4138 5209 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 580 399 365 159 132
128 97 85 60
BspEI T'CCGG_A
Cuts at: 5655 5655
Size: 5992
BspHI T'CATG_A
Cuts at: 3881 5611 3881
Size: 1730 4262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3051 3347 844
Size: 2207 296 3489
Fragments arranged by size:
3489 2207 296
BsrBI CCG'CTC
Cuts at: 1394 1823 2467 2896 3210 3879 1394
Size: 429 644 429 314 669 3507
Fragments arranged by size:
3507 669 644 429 429 314
BsrDI GCAATG_nn'
Cuts at: 65 1248 4487 4661 65
Size: 1183 3239 174 1396
Fragments arranged by size:
3239 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2235 2578 3118 4636 4823 1700
Size: 110 425 343 540 1518 187 2869
Fragments arranged by size:
2869 1518 540 425 343 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5992
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5992
BssSI C'ACGA_G
Cuts at: 2219 4052 5064 2219
Size: 1833 1012 3147
Fragments arranged by size:
3147 1833 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5992
BstBI TT'CG_AA
Cuts at: 3067 4796 3067
Size: 1729 4263
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5992
BstXI CCAn_nnnn'nTGG
Cuts at: 2455 2455
Size: 5992
BstYI r'GATC_y
Cuts at: 1972 3021 3028 3057 3322 3689 4074 4091 5543
Size: 1049 7 29 265 367 385 17 1452
Cuts at: 5543 5629 5641 5987 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1049 385 367 346 265 86
29 17 12 7
BtgI C'CryG_G
Cuts at: 513 1065 2157 2454 2796 513
Size: 552 1092 297 342 3709
Fragments arranged by size:
3709 1092 552 342 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2428 487
Size: 683 56 1202 4051
Fragments arranged by size:
4051 1202 683 56
BtsI GCAGTG_nn'
Cuts at: 2944 3200 3212 3237 3508 4302 4322 2944
Size: 256 12 25 271 794 20 4614
Fragments arranged by size:
4614 794 271 256 25 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5957
DraI TTT'AAA
Cuts at: 3296 3296
Size: 5992
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4999 809
Size: 507 3683 1802
Fragments arranged by size:
3683 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2457 2475
Size: 54 1425 50 275 18 378 249 18
Cuts at: 2475 2553 2754 2844 3439 4328 5957 8
Size: 78 201 90 595 889 1629 43
Fragments arranged by size:
1629 1425 889 595 378 275 249 201
90 78 54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3439 1537
Size: 275 1627 4090
Fragments arranged by size:
4090 1627 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2671 3922 1353
Size: 1318 1251 3423
Fragments arranged by size:
3423 1318 1251
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2083 4579 4953 5099 387
Size: 1064 138 494 2496 374 146 1280
Fragments arranged by size:
2496 1280 1064 494 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 3133 3207 4055 4652 5439 5707
Size: 393 477 1024 74 848 597 787 268
Cuts at: 5707 1239
Size: 1524
Fragments arranged by size:
1524 1024 848 787 597 477 393 268
74
EcoO109I rG'GnC_Cy
Cuts at: 2767 2767
Size: 5992
EcoRI G'AATT_C
Cuts at: 3301 3679 3301
Size: 378 5614
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2281 2281
Size: 5992
FspI TGC'GCA
Cuts at: 4498 4498
Size: 5992
HaeII r_GCGC'y
Cuts at: 1678 2084 2242 2294 2454 2474 2489 2729 5139
Size: 406 158 52 160 20 15 240 2410
Cuts at: 5139 1678
Size: 2531
Fragments arranged by size:
2531 2410 406 240 160 158 52 20
15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 2911 2943 3013
Size: 32 135 32 560 32 747 32 70
Cuts at: 3013 3045 3139 3171 3194 3226 4633 4665 4707
Size: 32 94 32 23 32 1407 32 42
Cuts at: 4707 4739 1373
Size: 32 2626
Fragments arranged by size:
2626 1407 747 560 135 94 70 42
32 32 32 32 32 32 32 32
32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 3336 3582 669
Size: 988 281 1398 246 3079
Fragments arranged by size:
3079 1398 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5992
HpaI GTT'AAC
Cuts at: 3582 3582
Size: 5992
KasI G'GCGC_C
Cuts at: 2080 2238 2485 2080
Size: 158 247 5587
Fragments arranged by size:
5587 247 158
KpnI G_GTAC'C
Cuts at: 3317 3317
Size: 5992
MfeI C'AATT_G
Cuts at: 3591 3591
Size: 5992
MluI A'CGCG_T
Cuts at: 2516 5868 2516
Size: 3352 2640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5106 5290 1305
Size: 3801 184 2007
Fragments arranged by size:
3801 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 2477 2756 10
Size: 54 1425 343 378 267 279 3246
Fragments arranged by size:
3246 1425 378 343 279 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 2339 2496 2870 3950 4309 4468
Size: 648 906 267 157 374 1080 359 159
Cuts at: 4468 518
Size: 2042
Fragments arranged by size:
2042 1080 906 648 374 359 267 159
157
MspA1I CmG'CkG
Cuts at: 1606 2781 3408 4085 5233 5478 1606
Size: 1175 627 677 1148 245 2120
Fragments arranged by size:
2120 1175 1148 677 627 245
NaeI GCC'GGC
Cuts at: 3120 3120
Size: 5992
NarI GG'CG_CC
Cuts at: 2081 2239 2486 2081
Size: 158 247 5587
Fragments arranged by size:
5587 247 158
NcoI C'CATG_G
Cuts at: 513 1065 2454 513
Size: 552 1389 4051
Fragments arranged by size:
4051 1389 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5992
NgoMIV G'CCGG_C
Cuts at: 3118 3118
Size: 5992
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5992
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 2077 3350 4895 1132
Size: 97 848 1273 1545 2229
Fragments arranged by size:
2229 1545 1273 848 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5992
PciI A'CATG_T
Cuts at: 4891 4891
Size: 5992
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2132 3025 1108
Size: 1024 893 4075
Fragments arranged by size:
4075 1024 893
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4061 4093 5574 5606 4061
Size: 32 1481 32 4447
Fragments arranged by size:
4447 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5992
PsiI TTA'TAA
Cuts at: 3562 3562
Size: 5992
PstI C_TGCA'G
Cuts at: 830 3344 830
Size: 2514 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5992
SalI G'TCGA_C
Cuts at: 1936 3334 1936
Size: 1398 4594
SapI GCTCTTCn'nnn_
Cuts at: 2671 2671
Size: 5992
SbfI CC_TGCA'GG
Cuts at: 3344 3344
Size: 5992
ScaI AGT'ACT
Cuts at: 4240 5952 4240
Size: 1712 4280
SexAI A'CCwGG_T
Cuts at: 2301 2301
Size: 5992
SfcI C'TryA_G
Cuts at: 826 1045 3340 4475 5156 5347 826
Size: 219 2295 1135 681 191 1471
Fragments arranged by size:
2295 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2082 2240 2487 2082
Size: 158 247 5587
Fragments arranged by size:
5587 247 158
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 2018 2617 3319 1872
Size: 146 599 702 4545
Fragments arranged by size:
4545 702 599 146
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4096 4997 5259 820
Size: 197 926 2153 901 262 1553
Fragments arranged by size:
2153 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3783 493
Size: 3290 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 3350 1132
Size: 2218 3774
SspI AAT'ATT
Cuts at: 5 52 3916 5
Size: 47 3864 2081
Fragments arranged by size:
3864 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5992
StyI C'CwwG_G
Cuts at: 513 1065 2454 2790 3032 3288 3431 513
Size: 552 1389 336 242 256 143 3074
Fragments arranged by size:
3074 1389 552 336 256 242 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2852 2869 3194 4019 4036 4189 4374
Size: 1255 1272 17 325 825 17 153 185
Cuts at: 4374 4819 325
Size: 445 1498
Fragments arranged by size:
1498 1272 1255 825 445 325 185 153
17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2598 4238 5950
Size: 274 80 33 51 1443 621 1640 1712
Cuts at: 5950 96
Size: 138
Fragments arranged by size:
1712 1640 1443 621 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2107 2139 5952 5984 2107
Size: 32 3813 32 2115
Fragments arranged by size:
3813 2115 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2303 1316
Size: 987 5005
XbaI T'CTAG_A
Cuts at: 3328 3328
Size: 5992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2767 2854 2767
Size: 87 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5992
XmaI C'CCGG_G
Cuts at: 1870 2016 2615 3317 1870
Size: 146 599 702 4545
Fragments arranged by size:
4545 702 599 146
XmnI GAAnn'nnTTC
Cuts at: 2264 4121 2264
Size: 1857 4135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3800 276
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI
SrfI SwaI