Restriction map for
pFN21AE3526
(Circular) (Six-base) MAPSORT of: pFN21AE3526.seq Check: 8639 from: 1 to: 7813
pFN21AE3526 7813 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3808 3808
Size: 7813
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2159 3579 3939 5623
Size: 53 83 186 936 623 1420 360 1684
Cuts at: 5623 278
Size: 2468
Fragments arranged by size:
2468 1684 1420 936 623 360 186 83
53
AccI GT'mk_AC
Cuts at: 1937 5156 1937
Size: 3219 4594
Acc65I G'GTAC_C
Cuts at: 2562 5134 2562
Size: 2572 5241
AclI AA'CG_TT
Cuts at: 1563 5940 6313 1563
Size: 4377 373 3063
Fragments arranged by size:
4377 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 2297 3827 4595 4991 5876 7260 7528
Size: 404 261 1530 768 396 885 1384 268
Cuts at: 7528 1632
Size: 1917
Fragments arranged by size:
1917 1530 1384 885 768 404 396 268
261
AfeI AGC'GCT
Cuts at: 2964 3401 2964
Size: 437 7376
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7616
AflIII A'CryG_T
Cuts at: 1203 3351 6712 7689 1203
Size: 2148 3361 977 1327
Fragments arranged by size:
3361 2148 1327 977
AgeI A'CCGG_T
Cuts at: 6644 6644
Size: 7813
AhdI GACnn_n'nnGTC
Cuts at: 6542 6542
Size: 7813
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7813
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3978 4377 1184
Size: 2794 399 4620
Fragments arranged by size:
4620 2794 399
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3689 3721 3689
Size: 32 7781
AlwNI CAG_nnn'CTG
Cuts at: 3313 4184 4849 5556 7128 3313
Size: 871 665 707 1572 3998
Fragments arranged by size:
3998 1572 871 707 665
ApaI G_GGCC'C
Cuts at: 2603 3932 4520 4604 4694 2603
Size: 1329 588 84 90 5722
Fragments arranged by size:
5722 1329 588 90 84
ApaLI G'TGCA_C
Cuts at: 2111 2771 4589 5870 7026 2111
Size: 660 1818 1281 1156 2898
Fragments arranged by size:
2898 1818 1281 1156 660
ApoI r'AATT_y
Cuts at: 1493 3329 5122 5348 5500 7609 1493
Size: 1836 1793 226 152 2109 1697
Fragments arranged by size:
2109 1836 1793 1697 226 152
AscI GG'CGCG_CC
Cuts at: 4077 4077
Size: 7813
AseI AT'TA_AT
Cuts at: 160 1032 6367 160
Size: 872 5335 1606
Fragments arranged by size:
5335 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7813
AvaI C'yCGr_G
Cuts at: 1870 1943 2720 3425 3811 3993 5138 1870
Size: 73 777 705 386 182 1145 4545
Fragments arranged by size:
4545 1145 777 705 386 182 73
AvrII C'CTAG_G
Cuts at: 2944 2944
Size: 7813
BamHI G'GATC_C
Cuts at: 2995 3564 5143 5510 2995
Size: 569 1579 367 5298
Fragments arranged by size:
5298 1579 569 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2562 4064 4102 4345 5134
Size: 325 217 627 775 1502 38 243 789
Cuts at: 5134 6589 618
Size: 1455 1842
Fragments arranged by size:
1842 1502 1455 789 775 627 325 243
217 38
BanII G_rGCy'C
Cuts at: 1391 2603 2727 3483 3650 3932 4208 4520 4604
Size: 1212 124 756 167 282 276 312 84
Cuts at: 4604 4619 4694 4737 5132 1391
Size: 15 75 43 395 4072
Fragments arranged by size:
4072 1212 756 395 312 282 276 167
124 84 75 43 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3088 4683 928
Size: 971 1189 1595 4058
Fragments arranged by size:
4058 1595 1189 971
BbvCI CC'TCA_GC
Cuts at: 2078 2078
Size: 7813
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6004 6038 1903
Size: 34 50 34 3983 34 3678
Fragments arranged by size:
3983 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3550 5705 6915 3550
Size: 2155 1210 4448
Fragments arranged by size:
4448 2155 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7813
BfrBI ATG'CAT
Cuts at: 5110 5110
Size: 7813
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2534 2891 4087
Size: 107 122 71 631 605 862 357 1196
Cuts at: 4087 6424 136
Size: 2337 1525
Fragments arranged by size:
2337 1525 1196 862 631 605 357 122
107 71
BglII A'GATC_T
Cuts at: 3414 7808 3414
Size: 4394 3419
BlpI GC'TnA_GC
Cuts at: 2045 2196 2679 3969 4451 4736 5230 2045
Size: 151 483 1290 482 285 494 4628
Fragments arranged by size:
4628 1290 494 483 482 285 151
Bme1580I G_kGCm'C
Cuts at: 1790 2115 2603 2775 3932 4520 4593 4604 4694
Size: 325 488 172 1157 588 73 11 90
Cuts at: 4694 5086 5874 7030 1790
Size: 392 788 1156 2573
Fragments arranged by size:
2573 1157 1156 788 588 488 392 325
172 90 73 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7813
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3557 3998 4515
Size: 416 53 83 399 24 2140 441 517
Cuts at: 4515 5544 6502 442
Size: 1029 958 1753
Fragments arranged by size:
2140 1753 1029 958 517 441 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7813
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2044 3451 3934 2044
Size: 1407 483 5923
Fragments arranged by size:
5923 1407 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3431 3469 3545 3869 4820 4877 4895 6473
Size: 2192 38 76 324 951 57 18 1578
Cuts at: 6473 1239
Size: 2579
Fragments arranged by size:
2579 2192 1578 951 324 76 57 38
18
Bpu10I CC'TnA_GC
Cuts at: 2078 4202 4718 2078
Size: 2124 516 5173
Fragments arranged by size:
5173 2124 516
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2363 2894 3029 4462 5938 6803 7101 2363
Size: 531 135 1433 1476 865 298 3075
Fragments arranged by size:
3075 1476 1433 865 531 298 135
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2459 3422 4805 6476 882
Size: 617 960 963 1383 1671 2219
Fragments arranged by size:
2219 1671 1383 963 960 617
BsaAI yAC'GTr
Cuts at: 493 1204 5604 493
Size: 711 4400 2702
Fragments arranged by size:
4400 2702 711
BsaBI GATnn'nnATC
Cuts at: 2618 7807 2618
Size: 5189 2624
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2156 2576 2940 3576
Size: 53 83 186 936 623 420 364 636
Cuts at: 3576 3891 3936 4436 4493 5620 6002 6637 275
Size: 315 45 500 57 1127 382 635 1451
Fragments arranged by size:
1451 1127 936 636 635 623 500 420
382 364 315 186 83 57 53 45
BsaWI w'CCGG_w
Cuts at: 3707 6246 6644 6918 7065 7476 3707
Size: 2539 398 274 147 411 4044
Fragments arranged by size:
4044 2539 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2546 2576 3620 3650 1178
Size: 30 165 30 1143 30 1044 30 5341
Fragments arranged by size:
5341 1143 1044 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2765 2915 4238 4286 4390 4817 4847 4892
Size: 1579 150 1323 48 104 427 30 45
Cuts at: 4892 4901 1186
Size: 9 4098
Fragments arranged by size:
4098 1579 1323 427 150 104 48 45
30 9
BseYI C'CCAG_C
Cuts at: 2051 2999 3214 3671 4092 4246 5091 7016 7512
Size: 948 215 457 421 154 845 1925 496
Cuts at: 7512 2051
Size: 2352
Fragments arranged by size:
2352 1925 948 845 496 457 421 215
154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2733 3163 3476 1163
Size: 573 997 430 313 5500
Fragments arranged by size:
5500 997 573 430 313
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3015 3157 4432 5263 6024 7052
Size: 275 186 1014 142 1275 831 761 1028
Cuts at: 7052 1540
Size: 2301
Fragments arranged by size:
2301 1275 1028 1014 831 761 275 186
142
BsiHKAI G_wGCw'C
Cuts at: 2115 2271 2355 2409 2685 2775 2789 2859 3011
Size: 156 84 54 276 90 14 70 152
Cuts at: 3011 3033 3483 3596 4208 4457 4593 4737 5874
Size: 22 450 113 612 249 136 144 1137
Cuts at: 5874 5959 7030 2115
Size: 85 1071 2898
Fragments arranged by size:
2898 1137 1071 612 450 276 249 156
152 144 136 113 90 85 84 70
54 22 14
BsmI GAATG_Cn'
Cuts at: 5324 5417 5324
Size: 93 7720
BsmBI CGTCTCn'nnnn_
Cuts at: 3741 4829 3741
Size: 1088 6725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2271 2355 2409 2603 2685 2727
Size: 399 325 156 84 54 194 82 42
Cuts at: 2727 2775 2789 2859 3011 3033 3483 3596 3650
Size: 48 14 70 152 22 450 113 54
Cuts at: 3650 3932 4208 4457 4520 4593 4604 4619 4694
Size: 282 276 249 63 73 11 15 75
Cuts at: 4694 4737 5086 5132 5874 5959 7030 1391
Size: 43 349 46 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 450 399 349 325 282
276 249 194 156 152 113 85 84
82 75 73 70 63 54 54 48
46 43 42 22 15 14 11
BspEI T'CCGG_A
Cuts at: 7476 7476
Size: 7813
BspHI T'CATG_A
Cuts at: 3360 5076 5702 7432 3360
Size: 1716 626 1730 3741
Fragments arranged by size:
3741 1730 1716 626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2313 2530 2940 3252 3808 4455 4935 5168
Size: 1469 217 410 312 556 647 480 233
Cuts at: 5168 844
Size: 3489
Fragments arranged by size:
3489 1469 647 556 480 410 312 233
217
BsrBI CCG'CTC
Cuts at: 1394 1823 2200 2231 3175 4108 4891 5700 1394
Size: 429 377 31 944 933 783 809 3507
Fragments arranged by size:
3507 944 933 809 783 429 377 31
BsrDI GCAATG_nn'
Cuts at: 65 1248 5043 6308 6482 65
Size: 1183 3795 1265 174 1396
Fragments arranged by size:
3795 1396 1265 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2184 2256 3446 3988 4086 6457 6644
Size: 110 374 72 1190 542 98 2371 187
Cuts at: 6644 1700
Size: 2869
Fragments arranged by size:
2869 2371 1190 542 374 187 110 98
72
BsrGI T'GTAC_A
Cuts at: 96 2664 96
Size: 2568 5245
BssHII G'CGCG_C
Cuts at: 1925 2627 4077 1925
Size: 702 1450 5661
Fragments arranged by size:
5661 1450 702
BssSI C'ACGA_G
Cuts at: 2033 3127 5873 6885 2033
Size: 1094 2746 1012 2961
Fragments arranged by size:
2961 2746 1094 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2779 3499 794
Size: 1985 720 5108
Fragments arranged by size:
5108 1985 720
BstBI TT'CG_AA
Cuts at: 6617 6617
Size: 7813
BstEII G'GTnAC_C
Cuts at: 1184 3301 3729 1184
Size: 2117 428 5268
Fragments arranged by size:
5268 2117 428
BstXI CCAn_nnnn'nTGG
Cuts at: 3631 4627 4798 3631
Size: 996 171 6646
Fragments arranged by size:
6646 996 171
BstYI r'GATC_y
Cuts at: 1972 2995 3414 3564 5143 5510 5895 5912 7364
Size: 1023 419 150 1579 367 385 17 1452
Cuts at: 7364 7450 7462 7808 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1579 1452 1023 419 385 367 346
150 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3569 4532 3569
Size: 963 6850
BtgI C'CryG_G
Cuts at: 513 1065 2367 2655 3101 3873 4026 4162 4266
Size: 552 1302 288 446 772 153 136 104
Cuts at: 4266 4341 4349 4426 4580 4956 513
Size: 75 8 77 154 376 3370
Fragments arranged by size:
3370 1302 772 552 446 376 288 154
153 136 104 77 75 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3277 487
Size: 683 56 2051 5023
Fragments arranged by size:
5023 2051 683 56
BtsI GCAGTG_nn'
Cuts at: 2062 3270 3497 4260 4593 5329 6123 6143 2062
Size: 1208 227 763 333 736 794 20 3732
Fragments arranged by size:
3732 1208 794 763 736 333 227 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7813
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3090 3125 529
Size: 35 2526 35 5217
Fragments arranged by size:
5217 2526 35 35
DraI TTT'AAA
Cuts at: 5117 5117
Size: 7813
DraIII CAC_nnn'GTG
Cuts at: 4163 4163
Size: 7813
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6820 809
Size: 507 5504 1802
Fragments arranged by size:
5504 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2128 2535 2883
Size: 54 1425 50 275 18 298 407 348
Cuts at: 2883 3012 3154 3315 3849 3978 3986 4023 4429
Size: 129 142 161 534 129 8 37 406
Cuts at: 4429 5260 6149 7778 8
Size: 831 889 1629 43
Fragments arranged by size:
1629 1425 889 831 534 407 406 348
298 275 161 142 129 129 54 50
43 37 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 3012 3154 4429 5260 1537
Size: 275 1200 142 1275 831 4090
Fragments arranged by size:
4090 1275 1200 831 275 142
EarI CTCTTCn'nnn_
Cuts at: 1353 2085 3200 3242 4552 5743 1353
Size: 732 1115 42 1310 1191 3423
Fragments arranged by size:
3423 1310 1191 1115 732 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4383 6400 6774 6920 387
Size: 1064 138 2794 2017 374 146 1280
Fragments arranged by size:
2794 2017 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3481 4206 4735 3481
Size: 725 529 6559
Fragments arranged by size:
6559 725 529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2297 3431 3469 3545 3827 3869
Size: 393 404 261 1134 38 76 282 42
Cuts at: 3869 4595 4820 4877 4895 4991 5876 6473 7260
Size: 726 225 57 18 96 885 597 787
Cuts at: 7260 7528 1239
Size: 268 1524
Fragments arranged by size:
1524 1134 885 787 726 597 404 393
282 268 261 225 96 76 57 42
38 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2129 3130 3248 2129
Size: 1001 118 6694
Fragments arranged by size:
6694 1001 118
EcoO109I rG'GnC_Cy
Cuts at: 2302 2599 2600 3551 3777 3929 4517 4600 4601
Size: 297 1 951 226 152 588 83 1
Cuts at: 4601 4684 4753 2302
Size: 83 69 5362
Fragments arranged by size:
5362 951 588 297 226 152 83 83
69 1 1
EcoRI G'AATT_C
Cuts at: 5122 5500 5122
Size: 378 7435
FseI GG_CCGG'CC
Cuts at: 3992 4090 3992
Size: 98 7715
FspI TGC'GCA
Cuts at: 2438 6319 2438
Size: 3881 3932
HaeII r_GCGC'y
Cuts at: 1678 2072 2966 3189 3403 6960 1678
Size: 394 894 223 214 3557 2531
Fragments arranged by size:
3557 2531 894 394 223 214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2598 2630 2744 2776 3620
Size: 32 135 32 1026 32 114 32 844
Cuts at: 3620 3652 4166 4198 6454 6486 6528 6560 1373
Size: 32 514 32 2256 32 42 32 2626
Fragments arranged by size:
2626 2256 1026 844 514 135 114 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5157 5403 669
Size: 988 281 3219 246 3079
Fragments arranged by size:
3219 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7813
HpaI GTT'AAC
Cuts at: 5403 5403
Size: 7813
KpnI G_GTAC'C
Cuts at: 2566 5138 2566
Size: 2572 5241
MfeI C'AATT_G
Cuts at: 5412 5412
Size: 7813
MluI A'CGCG_T
Cuts at: 7689 7689
Size: 7813
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3444 3764 5034 6927 7111 1305
Size: 2139 320 1270 1893 184 2007
Fragments arranged by size:
2139 2007 1893 1270 320 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2130 2885 3317 3851 4025
Size: 54 1425 343 298 755 432 534 174
Cuts at: 4025 10
Size: 3798
Fragments arranged by size:
3798 1425 755 534 432 343 298 174
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2660 4925 5771 6130 6289 518
Size: 648 1494 2265 846 359 159 2042
Fragments arranged by size:
2265 2042 1494 846 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2195 2841 3017 3523 3671 3962 3973 4096
Size: 589 646 176 506 148 291 11 123
Cuts at: 4096 4153 4268 4428 5095 5229 5906 7054 7299
Size: 57 115 160 667 134 677 1148 245
Cuts at: 7299 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 667 646 589 506 291
245 176 160 148 134 123 115 57
11
NaeI GCC'GGC
Cuts at: 2258 3448 3990 4088 2258
Size: 1190 542 98 5983
Fragments arranged by size:
5983 1190 542 98
NcoI C'CATG_G
Cuts at: 513 1065 2655 3873 513
Size: 552 1590 1218 4453
Fragments arranged by size:
4453 1590 1218 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7813
NgoMIV G'CCGG_C
Cuts at: 2256 3446 3988 4086 2256
Size: 1190 542 98 5983
Fragments arranged by size:
5983 1190 542 98
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7813
NotI GC'GGCC_GC
Cuts at: 3154 5260 3154
Size: 2106 5707
NsiI A_TGCA'T
Cuts at: 5112 5112
Size: 7813
NspI r_CATG'y
Cuts at: 1132 1229 3355 3397 5114 5171 6716 1132
Size: 97 2126 42 1717 57 1545 2229
Fragments arranged by size:
2229 2126 1717 1545 97 57 42
PasI CC'CwG_GG
Cuts at: 1348 3617 1348
Size: 2269 5544
PciI A'CATG_T
Cuts at: 3351 6712 3351
Size: 3361 4452
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2701 3313 4099 1108
Size: 1593 612 786 4822
Fragments arranged by size:
4822 1593 786 612
PfoI T'CCnGG_A
Cuts at: 2868 3885 2868
Size: 1017 6796
PmeI GTTT'AAAC
Cuts at: 5117 5117
Size: 7813
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4802 4834 5882 5914 7395 7427 4802
Size: 32 1048 32 1481 32 5188
Fragments arranged by size:
5188 1481 1048 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2302 3551 3777 4753 2302
Size: 1249 226 976 5362
Fragments arranged by size:
5362 1249 976 226
PshAI GACnn'nnGTC
Cuts at: 1613 4312 1613
Size: 2699 5114
PsiI TTA'TAA
Cuts at: 5383 5383
Size: 7813
PspOMI G'GGCC_C
Cuts at: 2599 3928 4516 4600 4690 2599
Size: 1329 588 84 90 5722
Fragments arranged by size:
5722 1329 588 90 84
PspXI vC'TCGA_Gb
Cuts at: 2720 3811 2720
Size: 1091 6722
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2657 2689 4112 4144 2657
Size: 32 1423 32 6326
Fragments arranged by size:
6326 1423 32 32
PstI C_TGCA'G
Cuts at: 830 2194 2784 2937 3252 3681 3967 4464 4770
Size: 1364 590 153 315 429 286 497 306
Cuts at: 4770 5165 830
Size: 395 3478
Fragments arranged by size:
3478 1364 590 497 429 395 315 306
286 153
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7813
PvuII CAG'CTG
Cuts at: 2195 3523 3671 3973 4096 5095 2195
Size: 1328 148 302 123 999 4913
Fragments arranged by size:
4913 1328 999 302 148 123
SacI G_AGCT'C
Cuts at: 3483 4208 4737 3483
Size: 725 529 6559
Fragments arranged by size:
6559 725 529
SacII CC_GC'GG
Cuts at: 4269 4429 4269
Size: 160 7653
SalI G'TCGA_C
Cuts at: 1936 5155 1936
Size: 3219 4594
SanDI GG'GwC_CC
Cuts at: 3551 3551
Size: 7813
SbfI CC_TGCA'GG
Cuts at: 2937 5165 2937
Size: 2228 5585
ScaI AGT'ACT
Cuts at: 6061 7773 6061
Size: 1712 6101
SfcI C'TryA_G
Cuts at: 826 1045 2190 2780 2933 3248 3677 3963 4460
Size: 219 1145 590 153 315 429 286 497
Cuts at: 4460 4766 5161 6296 6977 7168 826
Size: 306 395 1135 681 191 1471
Fragments arranged by size:
1471 1145 1135 681 590 497 429 395
315 306 286 219 191 153
SfiI GGCCn_nnn'nGGCC
Cuts at: 2891 2891
Size: 7813
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7813
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7813
SmaI CCC'GGG
Cuts at: 1872 3995 5140 1872
Size: 2123 1145 4545
Fragments arranged by size:
4545 2123 1145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2378 2720 2909 3044 3811 4477
Size: 197 926 435 342 189 135 767 666
Cuts at: 4477 5917 6818 7080 820
Size: 1440 901 262 1553
Fragments arranged by size:
1553 1440 926 901 767 666 435 342
262 197 189 135
SnaBI TAC'GTA
Cuts at: 493 5604 493
Size: 5111 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7813
SphI G_CATG'C
Cuts at: 1132 5171 1132
Size: 4039 3774
SrfI GCCC'GGGC
Cuts at: 3995 3995
Size: 7813
SspI AAT'ATT
Cuts at: 5 52 5737 5
Size: 47 5685 2081
Fragments arranged by size:
5685 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7813
StyI C'CwwG_G
Cuts at: 513 1065 2125 2298 2586 2655 2745 2889 2944
Size: 552 1060 173 288 69 90 144 55
Cuts at: 2944 3297 3873 4168 4554 5252 513
Size: 353 576 295 386 698 3074
Fragments arranged by size:
3074 1060 698 576 552 386 353 295
288 173 144 90 69 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2275 2483 3320 4501 5840 5857 6010
Size: 1255 695 208 837 1181 1339 17 153
Cuts at: 6010 6195 6640 325
Size: 185 445 1498
Fragments arranged by size:
1498 1339 1255 1181 837 695 445 208
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2664 6059 7771
Size: 274 80 33 51 1443 687 3395 1712
Cuts at: 7771 96
Size: 138
Fragments arranged by size:
3395 1712 1443 687 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7773 7805 7773
Size: 32 7781
Tth111I GACn'n_nGTC
Cuts at: 1316 2155 1316
Size: 839 6974
XbaI T'CTAG_A
Cuts at: 5149 5149
Size: 7813
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2059 2653 2698 2752 4139 4795 2059
Size: 594 45 54 1387 656 5077
Fragments arranged by size:
5077 1387 656 594 54 45
XhoI C'TCGA_G
Cuts at: 1943 2720 3811 1943
Size: 777 1091 5945
Fragments arranged by size:
5945 1091 777
XmaI C'CCGG_G
Cuts at: 1870 3993 5138 1870
Size: 2123 1145 4545
Fragments arranged by size:
4545 2123 1145
XmnI GAAnn'nnTTC
Cuts at: 2804 3368 5942 2804
Size: 564 2574 4675
Fragments arranged by size:
4675 2574 564
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2157 3577 3937 5621
Size: 53 83 186 936 623 1420 360 1684
Cuts at: 5621 276
Size: 2468
Fragments arranged by size:
2468 1684 1420 936 623 360 186 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
BaeI BbeI BsiWI BstZ17I EcoRV FalI FspAI KasI
NarI NruI PacI PmlI RsrII SapI SexAI SfoI
SwaI