Restriction map for
pFN21AE3524
(Circular) (Six-base) MAPSORT of: pFN21AE3524.seq Check: 6081 from: 1 to: 6415
pFN21AE3524 6415 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2460 3031 2460
Size: 571 5844
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4225 278
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 3758 1937
Size: 1149 672 4594
Fragments arranged by size:
4594 1149 672
Acc65I G'GTAC_C
Cuts at: 3736 3736
Size: 6415
AclI AA'CG_TT
Cuts at: 1563 4542 4915 1563
Size: 2979 373 3063
Fragments arranged by size:
3063 2979 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2174 2744 3008 3349 4478 5862 6130 1632
Size: 542 570 264 341 1129 1384 268 1917
Fragments arranged by size:
1917 1384 1129 570 542 341 268 264
AfeI AGC'GCT
Cuts at: 3247 3247
Size: 6415
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6218
AflIII A'CryG_T
Cuts at: 1203 2190 2595 2694 2823 5314 6291 1203
Size: 987 405 99 129 2491 977 1327
Fragments arranged by size:
2491 1327 987 977 405 129 99
AgeI A'CCGG_T
Cuts at: 5246 5246
Size: 6415
AhdI GACnn_n'nnGTC
Cuts at: 2122 5144 2122
Size: 3022 3393
AleI CACnn'nnGTG
Cuts at: 1166 2559 2942 1166
Size: 1393 383 4639
Fragments arranged by size:
4639 1393 383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6415
AlwNI CAG_nnn'CTG
Cuts at: 2440 2653 2782 4158 5730 2440
Size: 213 129 1376 1572 3125
Fragments arranged by size:
3125 1572 1376 213 129
ApaI G_GGCC'C
Cuts at: 2220 2220
Size: 6415
ApaLI G'TGCA_C
Cuts at: 2810 2945 4472 5628 2810
Size: 135 1527 1156 3597
Fragments arranged by size:
3597 1527 1156 135
ApoI r'AATT_y
Cuts at: 1493 3724 3950 4102 6211 1493
Size: 2231 226 152 2109 1697
Fragments arranged by size:
2231 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4969 160
Size: 872 3937 1606
Fragments arranged by size:
3937 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
AvaI C'yCGr_G
Cuts at: 1870 1943 3740 1870
Size: 73 1797 4545
Fragments arranged by size:
4545 1797 73
BamHI G'GATC_C
Cuts at: 3413 3745 4112 3413
Size: 332 367 5716
Fragments arranged by size:
5716 367 332
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3565 3736 5191 618
Size: 325 217 627 1778 171 1455 1842
Fragments arranged by size:
1842 1778 1455 627 325 217 171
BanII G_rGCy'C
Cuts at: 1391 2220 2361 3734 1391
Size: 829 141 1373 4072
Fragments arranged by size:
4072 1373 829 141
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2161 2798 3143 3355 928
Size: 971 262 637 345 212 3988
Fragments arranged by size:
3988 971 637 345 262 212
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4606 4640 1903
Size: 34 2669 34 3678
Fragments arranged by size:
3678 2669 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2534 4307 5517 2534
Size: 1773 1210 3432
Fragments arranged by size:
3432 1773 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6415
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5026 136
Size: 107 122 71 631 605 3354 1525
Fragments arranged by size:
3354 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6410 6410
Size: 6415
BlpI GC'TnA_GC
Cuts at: 3832 3832
Size: 6415
Bme1580I G_kGCm'C
Cuts at: 1790 2220 2814 2949 4476 5632 1790
Size: 430 594 135 1527 1156 2573
Fragments arranged by size:
2573 1527 1156 594 430 135
BmgBI CAC'GTC
Cuts at: 1318 2824 3361 1318
Size: 1506 537 4372
Fragments arranged by size:
4372 1506 537
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2655 4146 5104
Size: 416 53 83 399 24 1238 1491 958
Cuts at: 5104 442
Size: 1753
Fragments arranged by size:
1753 1491 1238 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3056 3091 3243 3244 3498 3513 5075 1239
Size: 1817 35 152 1 254 15 1562 2579
Fragments arranged by size:
2579 1817 1562 254 152 35 15 1
Bpu10I CC'TnA_GC
Cuts at: 3443 3443
Size: 6415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4540 5405 5703 4540
Size: 865 298 5252
Fragments arranged by size:
5252 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2315 5078 882
Size: 617 816 2763 2219
Fragments arranged by size:
2763 2219 816 617
BsaAI yAC'GTr
Cuts at: 493 1204 2809 4206 493
Size: 711 1605 1397 2702
Fragments arranged by size:
2702 1605 1397 711
BsaBI GATnn'nnATC
Cuts at: 6409 6409
Size: 6415
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2229 4222 4604 5239
Size: 53 83 186 936 696 1993 382 635
Cuts at: 5239 275
Size: 1451
Fragments arranged by size:
1993 1451 936 696 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4848 5246 5520 5667 6078 4848
Size: 398 274 147 411 5185
Fragments arranged by size:
5185 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2835 2865 3029 3059 3217
Size: 30 165 30 1432 30 164 30 158
Cuts at: 3217 3247 1178
Size: 30 4346
Fragments arranged by size:
4346 1432 165 164 158 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3246 1186
Size: 2060 4355
BseYI C'CCAG_C
Cuts at: 2242 2913 5618 6114 2242
Size: 671 2705 496 2543
Fragments arranged by size:
2705 2543 671 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2426 2945 1163
Size: 573 690 519 4633
Fragments arranged by size:
4633 690 573 519
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3627 3865 4626 5654 1540
Size: 275 186 1626 238 761 1028 2301
Fragments arranged by size:
2301 1626 1028 761 275 238 186
BsiHKAI G_wGCw'C
Cuts at: 2361 2814 2949 3449 4476 4561 5632 2361
Size: 453 135 500 1027 85 1071 3144
Fragments arranged by size:
3144 1071 1027 500 453 135 85
BsmI GAATG_Cn'
Cuts at: 2029 3209 3473 3926 4019 2029
Size: 1180 264 453 93 4425
Fragments arranged by size:
4425 1180 453 264 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2181 3516 2181
Size: 1335 5080
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2361 2814 2949 3449 3734 4476
Size: 399 430 141 453 135 500 285 742
Cuts at: 4476 4561 5632 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 500 453 430 399 285
141 135 85
BspEI T'CCGG_A
Cuts at: 6078 6078
Size: 6415
BspHI T'CATG_A
Cuts at: 4304 6034 4304
Size: 1730 4685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2460 2540 3031 3770 844
Size: 1616 80 491 739 3489
Fragments arranged by size:
3489 1616 739 491 80
BsrBI CCG'CTC
Cuts at: 1394 1823 2958 4302 1394
Size: 429 1135 1344 3507
Fragments arranged by size:
3507 1344 1135 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4910 5084 65
Size: 1183 3662 174 1396
Fragments arranged by size:
3662 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2889 3388 3538 5059 5246 1700
Size: 110 1079 499 150 1521 187 2869
Fragments arranged by size:
2869 1521 1079 499 187 150 110
BsrGI T'GTAC_A
Cuts at: 96 2685 3297 96
Size: 2589 612 3214
Fragments arranged by size:
3214 2589 612
BssHII G'CGCG_C
Cuts at: 1925 2161 1925
Size: 236 6179
BssSI C'ACGA_G
Cuts at: 2143 3596 4475 5487 2143
Size: 1453 879 1012 3071
Fragments arranged by size:
3071 1453 1012 879
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2257 2587 3248 794
Size: 1463 330 661 3961
Fragments arranged by size:
3961 1463 661 330
BstBI TT'CG_AA
Cuts at: 2491 5219 2491
Size: 2728 3687
BstEII G'GTnAC_C
Cuts at: 1184 2749 2872 1184
Size: 1565 123 4727
Fragments arranged by size:
4727 1565 123
BstXI CCAn_nnnn'nTGG
Cuts at: 2242 2561 3650 2242
Size: 319 1089 5007
Fragments arranged by size:
5007 1089 319
BstYI r'GATC_y
Cuts at: 1972 3413 3745 4112 4497 4514 5966 6052 6064
Size: 1441 332 367 385 17 1452 86 12
Cuts at: 6064 6410 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1441 385 367 346 332 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2640 2940 3643 513
Size: 552 1575 300 703 3285
Fragments arranged by size:
3285 1575 703 552 300
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5676
Fragments arranged by size:
5676 683 56
BtsI GCAGTG_nn'
Cuts at: 2554 2566 2971 3048 3292 3449 3546 3931 4725
Size: 12 405 77 244 157 97 385 794
Cuts at: 4725 4745 2554
Size: 20 4224
Fragments arranged by size:
4224 794 405 385 244 157 97 77
20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6415
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6380
DraI TTT'AAA
Cuts at: 3719 3719
Size: 6415
DraIII CAC_nnn'GTG
Cuts at: 2663 2663
Size: 6415
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3082 3359 5422 809
Size: 507 1766 277 2063 1802
Fragments arranged by size:
2063 1802 1766 507 277
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2097 2637
Size: 54 1425 50 275 18 198 69 540
Cuts at: 2637 3208 3390 3624 3862 4751 6380 8
Size: 571 182 234 238 889 1629 43
Fragments arranged by size:
1629 1425 889 571 540 275 238 234
198 182 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3624 3862 1537
Size: 275 1812 238 4090
Fragments arranged by size:
4090 1812 275 238
EarI CTCTTCn'nnn_
Cuts at: 1353 4345 1353
Size: 2992 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2123 5002 5376 5522 387
Size: 1064 138 534 2879 374 146 1280
Fragments arranged by size:
2879 1280 1064 534 374 146 138
EcoICRI GAG'CTC
Cuts at: 2359 2359
Size: 6415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2744 3008 3056 3091 3243 3244
Size: 393 542 570 264 48 35 152 1
Cuts at: 3244 3349 3498 3513 4478 5075 5862 6130 1239
Size: 105 149 15 965 597 787 268 1524
Fragments arranged by size:
1524 965 787 597 570 542 393 268
264 152 149 105 48 35 15 1
EcoNI CCTnn'n_nnAGG
Cuts at: 3038 3038
Size: 6415
EcoO109I rG'GnC_Cy
Cuts at: 2217 2779 2217
Size: 562 5853
EcoRI G'AATT_C
Cuts at: 3724 4102 3724
Size: 378 6037
FspI TGC'GCA
Cuts at: 3620 4921 3620
Size: 1301 5114
HaeII r_GCGC'y
Cuts at: 1678 3249 5562 1678
Size: 1571 2313 2531
Fragments arranged by size:
2531 2313 1571
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2140 2231 2263 2532
Size: 32 135 32 536 32 91 32 269
Cuts at: 2532 2564 3029 3061 3217 3249 5056 5088 5130
Size: 32 465 32 156 32 1807 32 42
Cuts at: 5130 5162 1373
Size: 32 2626
Fragments arranged by size:
2626 1807 536 465 269 156 135 91
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3759 4005 669
Size: 988 281 1821 246 3079
Fragments arranged by size:
3079 1821 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6415
HpaI GTT'AAC
Cuts at: 4005 4005
Size: 6415
KpnI G_GTAC'C
Cuts at: 3740 3740
Size: 6415
MfeI C'AATT_G
Cuts at: 4014 4014
Size: 6415
MluI A'CGCG_T
Cuts at: 2190 6291 2190
Size: 4101 2314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5529 5713 1305
Size: 4224 184 2007
Fragments arranged by size:
4224 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4593
Fragments arranged by size:
4593 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2559 2699 2942 3648 4373 4732 4891
Size: 648 1393 140 243 706 725 359 159
Cuts at: 4891 518
Size: 2042
Fragments arranged by size:
2042 1393 725 706 648 359 243 159
140
MspA1I CmG'CkG
Cuts at: 1606 2089 2113 2417 2440 2538 3028 3462 3831
Size: 483 24 304 23 98 490 434 369
Cuts at: 3831 4508 5656 5901 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 490 483 434 369 304
245 98 24 23
NaeI GCC'GGC
Cuts at: 3390 3390
Size: 6415
NcoI C'CATG_G
Cuts at: 513 1065 3643 513
Size: 552 2578 3285
Fragments arranged by size:
3285 2578 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6415
NgoMIV G'CCGG_C
Cuts at: 3388 3388
Size: 6415
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6415
NotI GC'GGCC_GC
Cuts at: 3862 3862
Size: 6415
NspI r_CATG'y
Cuts at: 1132 1229 2599 2698 3773 5318 1132
Size: 97 1370 99 1075 1545 2229
Fragments arranged by size:
2229 1545 1370 1075 99 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6415
PciI A'CATG_T
Cuts at: 2595 2694 5314 2595
Size: 99 2620 3696
Fragments arranged by size:
3696 2620 99
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2626 1108
Size: 1518 4897
PfoI T'CCnGG_A
Cuts at: 2736 3068 2736
Size: 332 6083
PmeI GTTT'AAAC
Cuts at: 3719 3719
Size: 6415
PmlI CAC'GTG
Cuts at: 2809 2809
Size: 6415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2465 2497 4484 4516 5997 6029 2465
Size: 32 1987 32 1481 32 2851
Fragments arranged by size:
2851 1987 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2779 2779
Size: 6415
PshAI GACnn'nnGTC
Cuts at: 1613 2417 1613
Size: 804 5611
PsiI TTA'TAA
Cuts at: 3985 3985
Size: 6415
PspOMI G'GGCC_C
Cuts at: 2216 2216
Size: 6415
PstI C_TGCA'G
Cuts at: 830 2262 2537 2561 3270 3767 830
Size: 1432 275 24 709 497 3478
Fragments arranged by size:
3478 1432 709 497 275 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6415
PvuII CAG'CTG
Cuts at: 2113 3028 2113
Size: 915 5500
SacI G_AGCT'C
Cuts at: 2361 2361
Size: 6415
SalI G'TCGA_C
Cuts at: 1936 3757 1936
Size: 1821 4594
SbfI CC_TGCA'GG
Cuts at: 3767 3767
Size: 6415
ScaI AGT'ACT
Cuts at: 2885 4663 6375 2885
Size: 1778 1712 2925
Fragments arranged by size:
2925 1778 1712
SfcI C'TryA_G
Cuts at: 826 1045 2258 2533 2557 3266 3310 3763 4898
Size: 219 1213 275 24 709 44 453 1135
Cuts at: 4898 5579 5770 826
Size: 681 191 1471
Fragments arranged by size:
1471 1213 1135 709 681 453 275 219
191 44 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
SgrAI Cr'CCGG_yG
Cuts at: 1700 3538 1700
Size: 1838 4577
SmaI CCC'GGG
Cuts at: 1872 3742 1872
Size: 1870 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4519 5420 5682 820
Size: 197 926 2576 901 262 1553
Fragments arranged by size:
2576 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4206 493
Size: 3713 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6415
SphI G_CATG'C
Cuts at: 1132 3773 1132
Size: 2641 3774
SspI AAT'ATT
Cuts at: 5 52 4339 5
Size: 47 4287 2081
Fragments arranged by size:
4287 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6415
StyI C'CwwG_G
Cuts at: 513 1065 2235 3409 3643 3854 513
Size: 552 1170 1174 234 211 3074
Fragments arranged by size:
3074 1174 1170 552 234 211
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2674 2768 2905 2911 3642 4442 4459
Size: 1255 1094 94 137 6 731 800 17
Cuts at: 4459 4612 4797 5242 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1094 800 731 445 185 153
137 94 17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2685 2883 2995
Size: 274 80 33 51 1443 708 198 112
Cuts at: 2995 3297 4661 6373 96
Size: 302 1364 1712 138
Fragments arranged by size:
1712 1443 1364 708 302 274 198 138
112 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2835 2867 3423 3455 6375 6407 2835
Size: 32 556 32 2920 32 2843
Fragments arranged by size:
2920 2843 556 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2228 1316
Size: 912 5503
XbaI T'CTAG_A
Cuts at: 3751 3751
Size: 6415
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6415
XmaI C'CCGG_G
Cuts at: 1870 3740 1870
Size: 1870 4545
XmnI GAAnn'nnTTC
Cuts at: 4544 4544
Size: 6415
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4223 276
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI