Restriction map for
pFN21AE3523
(Circular) (Six-base) MAPSORT of: pFN21AE3523.seq Check: 1944 from: 1 to: 5752
pFN21AE3523 5752 bp
With 182 enzymes: SgfI *
November 18, 2016 17:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3095 1937
Size: 1158 4594
Acc65I G'GTAC_C
Cuts at: 2643 3073 2643
Size: 430 5322
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2330 2607 2946 2952 3815 5199 5467 1632
Size: 698 277 339 6 863 1384 268 1917
Fragments arranged by size:
1917 1384 863 698 339 277 268 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 2506 4651 5628 1203
Size: 1303 2145 977 1327
Fragments arranged by size:
2145 1327 1303 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AlwNI CAG_nnn'CTG
Cuts at: 3495 5067 3495
Size: 1572 4180
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 3061 3287 3439 5548 1493
Size: 1568 226 152 2109 1697
Fragments arranged by size:
2109 1697 1568 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 2589 3077 1870
Size: 73 646 488 4545
Fragments arranged by size:
4545 646 488 73
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2643 3073 4528 618
Size: 325 217 627 856 430 1455 1842
Fragments arranged by size:
1842 1455 856 627 430 325 217
BanII G_rGCy'C
Cuts at: 1391 2565 3071 1391
Size: 1174 506 4072
Fragments arranged by size:
4072 1174 506
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2940 928
Size: 971 1041 3740
Fragments arranged by size:
3740 1041 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2812 3644 4854 2812
Size: 832 1210 3710
Fragments arranged by size:
3710 1210 832
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4363 136
Size: 107 122 71 631 605 2691 1525
Fragments arranged by size:
2691 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2047 3813 4969 1790
Size: 257 1766 1156 2573
Fragments arranged by size:
2573 1766 1156 257
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2404 3483 4441
Size: 416 53 83 399 24 987 1079 958
Cuts at: 4441 442
Size: 1753
Fragments arranged by size:
1753 1079 987 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2801 4412 1239
Size: 1562 1611 2579
Fragments arranged by size:
2579 1611 1562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3877 4742 5040 3877
Size: 865 298 4589
Fragments arranged by size:
4589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2964 4415 882
Size: 617 1465 1451 2219
Fragments arranged by size:
2219 1465 1451 617
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 5746 5746
Size: 5752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3559 3941 4576 275
Size: 53 83 186 936 2026 382 635 1451
Fragments arranged by size:
2026 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4185 4583 4857 5004 5415 4185
Size: 398 274 147 411 4522
Fragments arranged by size:
4522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2065 2095 2927 2957 2963
Size: 30 165 30 662 30 832 30 6
Cuts at: 2963 2993 1178
Size: 30 3937
Fragments arranged by size:
3937 832 662 165 30 30 30 30
30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5752
BseYI C'CCAG_C
Cuts at: 2176 2606 2665 2800 2911 4955 5451 2176
Size: 430 59 135 111 2044 496 2477
Fragments arranged by size:
2477 2044 496 430 135 111 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2727 1163
Size: 573 991 4188
Fragments arranged by size:
4188 991 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2340 2565 3813 3898 4969 2340
Size: 225 1248 85 1071 3123
Fragments arranged by size:
3123 1248 1071 225 85
BsmI GAATG_Cn'
Cuts at: 2266 3263 3356 2266
Size: 997 93 4662
Fragments arranged by size:
4662 997 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2983 2983
Size: 5752
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2047 2340 2565 3071 3813 3898 4969
Size: 399 257 293 225 506 742 85 1071
Cuts at: 4969 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 506 399 293 257 225
85
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 3001 3641 5371 3001
Size: 640 1730 3382
Fragments arranged by size:
3382 1730 640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3107 844
Size: 2263 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 1394
Size: 429 1816 3507
Fragments arranged by size:
3507 1816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4247 4421 65
Size: 1183 2999 174 1396
Fragments arranged by size:
2999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2646 4396 4583 1700
Size: 110 836 1750 187 2869
Fragments arranged by size:
2869 1750 836 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5752
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstYI r'GATC_y
Cuts at: 1972 2101 3082 3449 3834 3851 5303 5389 5401
Size: 129 981 367 385 17 1452 86 12
Cuts at: 5401 5747 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 981 385 367 346 129 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2069 513
Size: 552 1004 4196
Fragments arranged by size:
4196 1004 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 2870 3268 4062 4082 2870
Size: 398 794 20 4540
Fragments arranged by size:
4540 794 398 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 2244 3056 2244
Size: 812 4940
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2825 3199 4088
Size: 54 1425 50 275 18 995 374 889
Cuts at: 4088 5717 8
Size: 1629 43
Fragments arranged by size:
1629 1425 995 889 374 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2091 2123 2626 3682 1353
Size: 738 32 503 1056 3423
Fragments arranged by size:
3423 1056 738 503 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2971 4339 4713 4859 387
Size: 1064 138 1382 1368 374 146 1280
Fragments arranged by size:
1382 1368 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2563 2563
Size: 5752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2607 2801 2946 2952 3815 4412
Size: 393 698 277 194 145 6 863 597
Cuts at: 4412 5199 5467 1239
Size: 787 268 1524
Fragments arranged by size:
1524 863 787 698 597 393 277 268
194 145 6
EcoO109I rG'GnC_Cy
Cuts at: 2482 2521 2482
Size: 39 5713
EcoRI G'AATT_C
Cuts at: 3061 3439 3061
Size: 378 5374
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 3018 4899 1678
Size: 1340 1881 2531
Fragments arranged by size:
2531 1881 1340
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2927 2959 2961 2963 2993
Size: 32 135 32 1355 32 2 2 30
Cuts at: 2993 2995 4393 4425 4467 4499 1373
Size: 2 1398 32 42 32 2626
Fragments arranged by size:
2626 1398 1355 135 42 32 32 32
32 32 30 2 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2741 3096 3342 669
Size: 988 281 803 355 246 3079
Fragments arranged by size:
3079 988 803 355 281 246
HindIII A'AGCT_T
Cuts at: 748 2298 748
Size: 1550 4202
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 2647 3077 2647
Size: 430 5322
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2787 4866 5050 1305
Size: 1482 2079 184 2007
Fragments arranged by size:
2079 2007 1482 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3930
Fragments arranged by size:
3930 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 3710 4069 4228 518
Size: 648 1089 1455 359 159 2042
Fragments arranged by size:
2042 1455 1089 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2779 3031 3168 3845 4993 5238 1606
Size: 1173 252 137 677 1148 245 2120
Fragments arranged by size:
2120 1173 1148 677 252 245 137
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5200
NdeI CA'TA_TG
Cuts at: 387 2699 387
Size: 2312 3440
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 2327 2395 3110 4655 1132
Size: 97 1098 68 715 1545 2229
Fragments arranged by size:
2229 1545 1098 715 97 68
PasI CC'CwG_GG
Cuts at: 1348 2022 1348
Size: 674 5078
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5752
PfoI T'CCnGG_A
Cuts at: 2428 2428
Size: 5752
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2521 2521
Size: 5752
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PstI C_TGCA'G
Cuts at: 830 3104 830
Size: 2274 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
PvuII CAG'CTG
Cuts at: 2779 3031 2779
Size: 252 5500
SacI G_AGCT'C
Cuts at: 2565 2565
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SapI GCTCTTCn'nnn_
Cuts at: 2123 2123
Size: 5752
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SfcI C'TryA_G
Cuts at: 826 1045 2811 3100 4235 4916 5107 826
Size: 219 1766 289 1135 681 191 1471
Fragments arranged by size:
1766 1471 1135 681 289 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 2591 3079 1872
Size: 719 488 4545
Fragments arranged by size:
4545 719 488
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3856 4757 5019 820
Size: 197 926 1913 901 262 1553
Fragments arranged by size:
1913 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 2233 152
Size: 2081 3671
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SrfI GCCC'GGGC
Cuts at: 2591 2591
Size: 5752
SspI AAT'ATT
Cuts at: 5 52 2332 2681 3676 5
Size: 47 2280 349 995 2081
Fragments arranged by size:
2280 2081 995 349 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5752
StyI C'CwwG_G
Cuts at: 513 1065 3191 513
Size: 552 2126 3074
Fragments arranged by size:
3074 2126 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 3779 3796 3949 4134 4579 325
Size: 1255 588 1611 17 153 185 445 1498
Fragments arranged by size:
1611 1498 1255 588 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3998 5710 96
Size: 274 80 33 51 1443 2021 1712 138
Fragments arranged by size:
2021 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2065 2097 2220 2252 5712 5744 2065
Size: 32 123 32 3460 32 2073
Fragments arranged by size:
3460 2073 123 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2975 1316
Size: 1659 4093
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 2589 3077 1870
Size: 719 488 4545
Fragments arranged by size:
4545 719 488
XmnI GAAnn'nnTTC
Cuts at: 3881 3881
Size: 5752
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SwaI
XcmI