Restriction map for
pFN21AE3521
(Circular) (Six-base) MAPSORT of: pFN21AE3521.seq Check: 4823 from: 1 to: 6070
pFN21AE3521 6070 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3880 278
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2440 2607 3413 1937
Size: 503 167 806 4594
Fragments arranged by size:
4594 806 503 167
Acc65I G'GTAC_C
Cuts at: 3391 3391
Size: 6070
AclI AA'CG_TT
Cuts at: 1563 4197 4570 1563
Size: 2634 373 3063
Fragments arranged by size:
3063 2634 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3338 4133 5517 5785 1632
Size: 1706 795 1384 268 1917
Fragments arranged by size:
1917 1706 1384 795 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5873
AflIII A'CryG_T
Cuts at: 1203 4969 5946 1203
Size: 3766 977 1327
Fragments arranged by size:
3766 1327 977
AgeI A'CCGG_T
Cuts at: 4901 4901
Size: 6070
AhdI GACnn_n'nnGTC
Cuts at: 4799 4799
Size: 6070
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6070
AlwNI CAG_nnn'CTG
Cuts at: 3813 5385 3813
Size: 1572 4498
ApaLI G'TGCA_C
Cuts at: 3037 4127 5283 3037
Size: 1090 1156 3824
Fragments arranged by size:
3824 1156 1090
ApoI r'AATT_y
Cuts at: 1493 3379 3605 3757 5866 1493
Size: 1886 226 152 2109 1697
Fragments arranged by size:
2109 1886 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4624 160
Size: 872 3592 1606
Fragments arranged by size:
3592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
AvaI C'yCGr_G
Cuts at: 1870 1943 2241 3395 1870
Size: 73 298 1154 4545
Fragments arranged by size:
4545 1154 298 73
AvrII C'CTAG_G
Cuts at: 2038 2038
Size: 6070
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2711 2744 2711
Size: 33 6037
BamHI G'GATC_C
Cuts at: 3400 3767 3400
Size: 367 5703
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2678 3391 4846 618
Size: 325 217 627 255 636 713 1455 1842
Fragments arranged by size:
1842 1455 713 636 627 325 255 217
BanII G_rGCy'C
Cuts at: 1391 2099 2286 2345 3389 1391
Size: 708 187 59 1044 4072
Fragments arranged by size:
4072 1044 708 187 59
BbeI G_GCGC'C
Cuts at: 2046 2046
Size: 6070
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5099
BbvCI CC'TCA_GC
Cuts at: 3340 3340
Size: 6070
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4261 4295 1903
Size: 34 50 34 2240 34 3678
Fragments arranged by size:
3678 2240 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2313 3962 5172 2313
Size: 1649 1210 3211
Fragments arranged by size:
3211 1649 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6070
BfrBI ATG'CAT
Cuts at: 2990 2990
Size: 6070
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4681 136
Size: 107 122 71 631 605 3009 1525
Fragments arranged by size:
3009 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6065 6065
Size: 6070
BlpI GC'TnA_GC
Cuts at: 3487 3487
Size: 6070
Bme1580I G_kGCm'C
Cuts at: 1790 3041 4131 5287 1790
Size: 1251 1090 1156 2573
Fragments arranged by size:
2573 1251 1156 1090
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3801 4759 442
Size: 416 53 83 399 24 2384 958 1753
Fragments arranged by size:
2384 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6070
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 6070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2375 2417 2628 4730 1239
Size: 1136 42 211 2102 2579
Fragments arranged by size:
2579 2102 1136 211 42
Bpu10I CC'TnA_GC
Cuts at: 3340 3340
Size: 6070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2435 4195 5060 5358 2435
Size: 1760 865 298 3147
Fragments arranged by size:
3147 1760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3227 4733 882
Size: 617 1728 1506 2219
Fragments arranged by size:
2219 1728 1506 617
BsaAI yAC'GTr
Cuts at: 493 1204 2550 3861 493
Size: 711 1346 1311 2702
Fragments arranged by size:
2702 1346 1311 711
BsaBI GATnn'nnATC
Cuts at: 6064 6064
Size: 6070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 3877 4259 4894
Size: 53 83 186 936 510 1834 382 635
Cuts at: 4894 275
Size: 1451
Fragments arranged by size:
1834 1451 936 635 510 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4503 4901 5175 5322 5733 4503
Size: 398 274 147 411 4840
Fragments arranged by size:
4840 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2165 2195 1178
Size: 30 165 30 762 30 5053
Fragments arranged by size:
5053 762 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2098 2102 2170 1186
Size: 912 4 68 5086
Fragments arranged by size:
5086 912 68 4
BseYI C'CCAG_C
Cuts at: 5273 5769 5273
Size: 496 5574
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2528 1163
Size: 573 792 4705
Fragments arranged by size:
4705 792 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3520 4281 5309 1540
Size: 275 186 1519 761 1028 2301
Fragments arranged by size:
2301 1519 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2099 3041 4131 4216 5287 2099
Size: 942 1090 85 1071 2882
Fragments arranged by size:
2882 1090 1071 942 85
BsmI GAATG_Cn'
Cuts at: 2847 2990 3581 3674 2847
Size: 143 591 93 5243
Fragments arranged by size:
5243 591 143 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2148 2148
Size: 6070
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 2286 2345 3041 3389 4131 4216
Size: 399 309 187 59 696 348 742 85
Cuts at: 4216 5287 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 696 399 348 309 187
85 59
BspEI T'CCGG_A
Cuts at: 5733 5733
Size: 6070
BspHI T'CATG_A
Cuts at: 2322 3959 5689 2322
Size: 1637 1730 2703
Fragments arranged by size:
2703 1730 1637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3425 844
Size: 2581 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3957 1394
Size: 429 2134 3507
Fragments arranged by size:
3507 2134 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2563 4565 4739 65
Size: 1183 1315 2002 174 1396
Fragments arranged by size:
2002 1396 1315 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2473 4714 4901 1700
Size: 110 663 2241 187 2869
Fragments arranged by size:
2869 2241 663 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6070
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6070
BssSI C'ACGA_G
Cuts at: 4130 5142 4130
Size: 1012 5058
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6070
BstBI TT'CG_AA
Cuts at: 4874 4874
Size: 6070
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6070
BstXI CCAn_nnnn'nTGG
Cuts at: 3074 3074
Size: 6070
BstYI r'GATC_y
Cuts at: 1972 3069 3400 3767 4152 4169 5621 5707 5719
Size: 1097 331 367 385 17 1452 86 12
Cuts at: 5719 6065 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1097 385 367 346 331 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2627 513
Size: 552 1562 3956
Fragments arranged by size:
3956 1562 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5331
Fragments arranged by size:
5331 683 56
BtsI GCAGTG_nn'
Cuts at: 2405 3586 4380 4400 2405
Size: 1181 794 20 4075
Fragments arranged by size:
4075 1181 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6035
DraI TTT'AAA
Cuts at: 3374 3374
Size: 6070
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5077 809
Size: 507 3761 1802
Fragments arranged by size:
3761 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 2402 2859
Size: 54 1425 50 275 18 432 140 457
Cuts at: 2859 3057 3517 4406 6035 8
Size: 198 460 889 1629 43
Fragments arranged by size:
1629 1425 889 460 457 432 275 198
140 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3517 1537
Size: 275 1705 4090
Fragments arranged by size:
4090 1705 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 3099 3200 3357 4000 1353
Size: 734 1012 101 157 643 3423
Fragments arranged by size:
3423 1012 734 643 157 101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2199 2210 3094 4657 5031 5177
Size: 1064 138 610 11 884 1563 374 146
Cuts at: 5177 387
Size: 1280
Fragments arranged by size:
1563 1280 1064 884 610 374 146 138
11
EcoICRI GAG'CTC
Cuts at: 2097 2097
Size: 6070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2375 2417 2628 3338 4133 4730 5517
Size: 393 743 42 211 710 795 597 787
Cuts at: 5517 5785 1239
Size: 268 1524
Fragments arranged by size:
1524 795 787 743 710 597 393 268
211 42
EcoRI G'AATT_C
Cuts at: 3379 3757 3379
Size: 378 5692
FspI TGC'GCA
Cuts at: 4576 4576
Size: 6070
HaeII r_GCGC'y
Cuts at: 1678 2046 5217 1678
Size: 368 3171 2531
Fragments arranged by size:
3171 2531 368
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2123 2155 2433 2447 2465
Size: 32 135 32 551 32 278 14 18
Cuts at: 2465 2479 2894 2926 3009 3041 4711 4743 4785
Size: 14 415 32 83 32 1670 32 42
Cuts at: 4785 4817 1373
Size: 32 2626
Fragments arranged by size:
2626 1670 551 415 278 135 83 42
32 32 32 32 32 32 32 18
14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2720 3414 3660 669
Size: 988 281 782 694 246 3079
Fragments arranged by size:
3079 988 782 694 281 246
HindIII A'AGCT_T
Cuts at: 748 3363 748
Size: 2615 3455
HpaI GTT'AAC
Cuts at: 3660 3660
Size: 6070
KasI G'GCGC_C
Cuts at: 2042 2042
Size: 6070
KpnI G_GTAC'C
Cuts at: 3395 3395
Size: 6070
MfeI C'AATT_G
Cuts at: 3669 3669
Size: 6070
MluI A'CGCG_T
Cuts at: 5946 5946
Size: 6070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5184 5368 1305
Size: 3879 184 2007
Fragments arranged by size:
3879 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2404 10
Size: 54 1425 343 572 3676
Fragments arranged by size:
3676 1425 572 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4028 4387 4546 518
Size: 648 2862 359 159 2042
Fragments arranged by size:
2862 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 3335 3486 4163 5311 5556 1606
Size: 408 1321 151 677 1148 245 2120
Fragments arranged by size:
2120 1321 1148 677 408 245 151
NarI GG'CG_CC
Cuts at: 2043 2043
Size: 6070
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5518
NdeI CA'TA_TG
Cuts at: 387 2811 3153 387
Size: 2424 342 3304
Fragments arranged by size:
3304 2424 342
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6070
NotI GC'GGCC_GC
Cuts at: 3517 3517
Size: 6070
NsiI A_TGCA'T
Cuts at: 2992 2992
Size: 6070
NspI r_CATG'y
Cuts at: 1132 1229 3092 3428 4973 1132
Size: 97 1863 336 1545 2229
Fragments arranged by size:
2229 1863 1545 336 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6070
PciI A'CATG_T
Cuts at: 4969 4969
Size: 6070
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6070
PmeI GTTT'AAAC
Cuts at: 3374 3374
Size: 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 5652 5684 4139
Size: 32 1481 32 4525
Fragments arranged by size:
4525 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6070
PsiI TTA'TAA
Cuts at: 3640 3640
Size: 6070
PstI C_TGCA'G
Cuts at: 830 3422 830
Size: 2592 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6070
PvuII CAG'CTG
Cuts at: 2014 3335 2014
Size: 1321 4749
SacI G_AGCT'C
Cuts at: 2099 2099
Size: 6070
SalI G'TCGA_C
Cuts at: 1936 3412 1936
Size: 1476 4594
SapI GCTCTTCn'nnn_
Cuts at: 2087 3099 2087
Size: 1012 5058
SbfI CC_TGCA'GG
Cuts at: 3422 3422
Size: 6070
ScaI AGT'ACT
Cuts at: 4318 6030 4318
Size: 1712 4358
SfcI C'TryA_G
Cuts at: 826 1045 3418 4553 5234 5425 826
Size: 219 2373 1135 681 191 1471
Fragments arranged by size:
2373 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2044 2044
Size: 6070
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6070
SmaI CCC'GGG
Cuts at: 1872 2243 3397 1872
Size: 371 1154 4545
Fragments arranged by size:
4545 1154 371
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2450 4174 5075 5337 820
Size: 197 926 507 1724 901 262 1553
Fragments arranged by size:
1724 1553 926 901 507 262 197
SnaBI TAC'GTA
Cuts at: 493 3861 493
Size: 3368 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6070
SphI G_CATG'C
Cuts at: 1132 3092 3428 1132
Size: 1960 336 3774
Fragments arranged by size:
3774 1960 336
SspI AAT'ATT
Cuts at: 5 52 3994 5
Size: 47 3942 2081
Fragments arranged by size:
3942 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6070
StyI C'CwwG_G
Cuts at: 513 1065 2038 3073 3509 513
Size: 552 973 1035 436 3074
Fragments arranged by size:
3074 1035 973 552 436
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4097 4114 4267 4452 4897 325
Size: 1255 2517 17 153 185 445 1498
Fragments arranged by size:
2517 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4316 6028 96
Size: 274 80 33 51 1443 2339 1712 138
Fragments arranged by size:
2339 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6030 6062 6030
Size: 32 6038
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6070
XbaI T'CTAG_A
Cuts at: 3406 3406
Size: 6070
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6070
XmaI C'CCGG_G
Cuts at: 1870 2241 3395 1870
Size: 371 1154 4545
Fragments arranged by size:
4545 1154 371
XmnI GAAnn'nnTTC
Cuts at: 4199 4199
Size: 6070
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3878 276
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BsiWI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI
XcmI