Restriction map for
pFN21AE3520
(Circular) (Six-base) MAPSORT of: pFN21AE3520.seq Check: 8855 from: 1 to: 5584
pFN21AE3520 5584 bp
With 182 enzymes: SgfI *
March 28, 2012 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3394 278
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2927 1937
Size: 990 4594
Acc65I G'GTAC_C
Cuts at: 2517 2905 2517
Size: 388 5196
AclI AA'CG_TT
Cuts at: 1563 3711 4084 1563
Size: 2148 373 3063
Fragments arranged by size:
3063 2148 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2396 2579 3647 5031 5299 1632
Size: 764 183 1068 1384 268 1917
Fragments arranged by size:
1917 1384 1068 764 268 183
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5387
AflIII A'CryG_T
Cuts at: 1203 4483 5460 1203
Size: 3280 977 1327
Fragments arranged by size:
3280 1327 977
AgeI A'CCGG_T
Cuts at: 4415 4415
Size: 5584
AhdI GACnn_n'nnGTC
Cuts at: 4313 4313
Size: 5584
AleI CACnn'nnGTG
Cuts at: 1166 2576 1166
Size: 1410 4174
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5584
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2486 2518 2486
Size: 32 5552
AlwNI CAG_nnn'CTG
Cuts at: 3327 4899 3327
Size: 1572 4012
ApaLI G'TGCA_C
Cuts at: 3641 4797 3641
Size: 1156 4428
ApoI r'AATT_y
Cuts at: 1493 2893 3119 3271 5380 1493
Size: 1400 226 152 2109 1697
Fragments arranged by size:
2109 1697 1400 226 152
AscI GG'CGCG_CC
Cuts at: 2745 2745
Size: 5584
AseI AT'TA_AT
Cuts at: 160 1032 4138 160
Size: 872 3106 1606
Fragments arranged by size:
3106 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
AvaI C'yCGr_G
Cuts at: 1870 1943 2336 2457 2531 2909 1870
Size: 73 393 121 74 378 4545
Fragments arranged by size:
4545 393 378 121 74 73
BamHI G'GATC_C
Cuts at: 2914 3281 2914
Size: 367 5217
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2517 2527 2762 2905 4360
Size: 325 217 627 730 10 235 143 1455
Cuts at: 4360 618
Size: 1842
Fragments arranged by size:
1842 1455 730 627 325 235 217 143
10
BanII G_rGCy'C
Cuts at: 1391 2316 2337 2903 1391
Size: 925 21 566 4072
Fragments arranged by size:
4072 925 566 21
BbeI G_GCGC'C
Cuts at: 2766 2766
Size: 5584
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2202 2476 928
Size: 971 303 274 4036
Fragments arranged by size:
4036 971 303 274
BbvCI CC'TCA_GC
Cuts at: 2154 2154
Size: 5584
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2286 2320 3775 3809 1903
Size: 34 349 34 1455 34 3678
Fragments arranged by size:
3678 1455 349 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3476 4686 3476
Size: 1210 4374
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5584
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4195 136
Size: 107 122 71 631 605 2523 1525
Fragments arranged by size:
2523 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5579 5579
Size: 5584
BlpI GC'TnA_GC
Cuts at: 3001 3001
Size: 5584
Bme1580I G_kGCm'C
Cuts at: 1790 3645 4801 1790
Size: 1855 1156 2573
Fragments arranged by size:
2573 1855 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5584
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3315 4273 442
Size: 416 53 83 399 24 1898 958 1753
Fragments arranged by size:
1898 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2660 4244 1239
Size: 845 576 1584 2579
Fragments arranged by size:
2579 1584 845 576
Bpu10I CC'TnA_GC
Cuts at: 2154 2154
Size: 5584
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3709 4574 4872 3709
Size: 865 298 4421
Fragments arranged by size:
4421 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4247 882
Size: 617 2748 2219
Fragments arranged by size:
2748 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3375 493
Size: 711 2171 2702
Fragments arranged by size:
2702 2171 711
BsaBI GATnn'nnATC
Cuts at: 5578 5578
Size: 5584
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2763 3391 3773 4408
Size: 53 83 186 936 1230 628 382 635
Cuts at: 4408 275
Size: 1451
Fragments arranged by size:
1451 1230 936 635 628 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2352 4017 4415 4689 4836 5247 2352
Size: 1665 398 274 147 411 2689
Fragments arranged by size:
2689 1665 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5359
Fragments arranged by size:
5359 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2345 2432 1186
Size: 1012 147 87 4338
Fragments arranged by size:
4338 1012 147 87
BseYI C'CCAG_C
Cuts at: 2726 4787 5283 2726
Size: 2061 496 3027
Fragments arranged by size:
3027 2061 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2326 1163
Size: 573 590 4421
Fragments arranged by size:
4421 590 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2397 2867 3034 3795 4823 1540
Size: 275 186 396 470 167 761 1028 2301
Fragments arranged by size:
2301 1028 761 470 396 275 186 167
BsiHKAI G_wGCw'C
Cuts at: 2118 2544 3645 3730 4801 2118
Size: 426 1101 85 1071 2901
Fragments arranged by size:
2901 1101 1071 426 85
BsmI GAATG_Cn'
Cuts at: 3095 3188 3095
Size: 93 5491
BsmBI CGTCTCn'nnnn_
Cuts at: 2384 2384
Size: 5584
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2316 2337 2544 2903 3645 3730
Size: 399 328 198 21 207 359 742 85
Cuts at: 3730 4801 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 359 328 207 198
85 21
BspEI T'CCGG_A
Cuts at: 5247 5247
Size: 5584
BspHI T'CATG_A
Cuts at: 3473 5203 3473
Size: 1730 3854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2490 2592 2939 844
Size: 1646 102 347 3489
Fragments arranged by size:
3489 1646 347 102
BsrBI CCG'CTC
Cuts at: 1394 1823 3471 1394
Size: 429 1648 3507
Fragments arranged by size:
3507 1648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4079 4253 65
Size: 1183 2831 174 1396
Fragments arranged by size:
2831 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4228 4415 1700
Size: 110 2418 187 2869
Fragments arranged by size:
2869 2418 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5584
BssHII G'CGCG_C
Cuts at: 1925 2730 2745 1925
Size: 805 15 4764
Fragments arranged by size:
4764 805 15
BssSI C'ACGA_G
Cuts at: 2302 3644 4656 2302
Size: 1342 1012 3230
Fragments arranged by size:
3230 1342 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2715 794
Size: 1921 3663
BstBI TT'CG_AA
Cuts at: 4388 4388
Size: 5584
BstEII G'GTnAC_C
Cuts at: 1184 2023 1184
Size: 839 4745
BstYI r'GATC_y
Cuts at: 1972 2914 3281 3666 3683 5135 5221 5233 5579
Size: 942 367 385 17 1452 86 12 346
Cuts at: 5579 1972
Size: 1977
Fragments arranged by size:
1977 1452 942 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2086 2248 2828 513
Size: 552 1021 162 580 3269
Fragments arranged by size:
3269 1021 580 552 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4845
Fragments arranged by size:
4845 683 56
BtsI GCAGTG_nn'
Cuts at: 2837 3100 3894 3914 2837
Size: 263 794 20 4507
Fragments arranged by size:
4507 794 263 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5584
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5549
DraI TTT'AAA
Cuts at: 2888 2888
Size: 5584
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4591 809
Size: 507 3275 1802
Fragments arranged by size:
3275 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2245 2394
Size: 54 1425 50 275 18 315 100 149
Cuts at: 2394 2864 3031 3920 5549 8
Size: 470 167 889 1629 43
Fragments arranged by size:
1629 1425 889 470 315 275 167 149
100 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2394 2864 3031 1537
Size: 275 582 470 167 4090
Fragments arranged by size:
4090 582 470 275 167
EarI CTCTTCn'nnn_
Cuts at: 1353 2322 2471 3514 1353
Size: 969 149 1043 3423
Fragments arranged by size:
3423 1043 969 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2120 2429 4171 4545 4691 387
Size: 1064 138 531 309 1742 374 146 1280
Fragments arranged by size:
1742 1280 1064 531 374 309 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2396 2579 2660 3647 4244 5031
Size: 393 452 312 183 81 987 597 787
Cuts at: 5031 5299 1239
Size: 268 1524
Fragments arranged by size:
1524 987 787 597 452 393 312 268
183 81
EcoO109I rG'GnC_Cy
Cuts at: 2461 2822 2461
Size: 361 5223
EcoRI G'AATT_C
Cuts at: 2893 3271 2893
Size: 378 5206
FspI TGC'GCA
Cuts at: 2094 4090 2094
Size: 1996 3588
FspAI rTGC'GCAy
Cuts at: 2094 2094
Size: 5584
HaeII r_GCGC'y
Cuts at: 1678 2766 2772 4731 1678
Size: 1088 6 1959 2531
Fragments arranged by size:
2531 1959 1088 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 4225 4257 4299
Size: 32 135 32 626 32 1995 32 42
Cuts at: 4299 4331 1373
Size: 32 2626
Fragments arranged by size:
2626 1995 626 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2928 3174 669
Size: 988 281 990 246 3079
Fragments arranged by size:
3079 990 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5584
HpaI GTT'AAC
Cuts at: 3174 3174
Size: 5584
KasI G'GCGC_C
Cuts at: 2762 2762
Size: 5584
KpnI G_GTAC'C
Cuts at: 2521 2909 2521
Size: 388 5196
MfeI C'AATT_G
Cuts at: 3183 3183
Size: 5584
MluI A'CGCG_T
Cuts at: 5460 5460
Size: 5584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4698 4882 1305
Size: 3393 184 2007
Fragments arranged by size:
3393 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3762
Fragments arranged by size:
3762 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2085 2576 2842 3542 3901 4060 518
Size: 648 919 491 266 700 359 159 2042
Fragments arranged by size:
2042 919 700 648 491 359 266 159
MspA1I CmG'CkG
Cuts at: 1606 2153 2374 2515 2623 2830 3000 3677 4825
Size: 547 221 141 108 207 170 677 1148
Cuts at: 4825 5070 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 547 245 221 207 170
141 108
NarI GG'CG_CC
Cuts at: 2763 2763
Size: 5584
NcoI C'CATG_G
Cuts at: 513 1065 2086 513
Size: 552 1021 4011
Fragments arranged by size:
4011 1021 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5584
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5584
NotI GC'GGCC_GC
Cuts at: 3031 3031
Size: 5584
NspI r_CATG'y
Cuts at: 1132 1229 2143 2942 4487 1132
Size: 97 914 799 1545 2229
Fragments arranged by size:
2229 1545 914 799 97
PasI CC'CwG_GG
Cuts at: 1348 2272 1348
Size: 924 4660
PciI A'CATG_T
Cuts at: 4483 4483
Size: 5584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5584
PmeI GTTT'AAAC
Cuts at: 2888 2888
Size: 5584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3653 3685 5166 5198 3653
Size: 32 1481 32 4039
Fragments arranged by size:
4039 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2461 2822 2461
Size: 361 5223
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5584
PsiI TTA'TAA
Cuts at: 3154 3154
Size: 5584
PstI C_TGCA'G
Cuts at: 830 2860 2936 830
Size: 2030 76 3478
Fragments arranged by size:
3478 2030 76
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5584
PvuII CAG'CTG
Cuts at: 2374 2374
Size: 5584
SacII CC_GC'GG
Cuts at: 2831 2831
Size: 5584
SalI G'TCGA_C
Cuts at: 1936 2926 1936
Size: 990 4594
SbfI CC_TGCA'GG
Cuts at: 2936 2936
Size: 5584
ScaI AGT'ACT
Cuts at: 3832 5544 3832
Size: 1712 3872
SfcI C'TryA_G
Cuts at: 826 1045 2752 2856 2932 4067 4748 4939 826
Size: 219 1707 104 76 1135 681 191 1471
Fragments arranged by size:
1707 1471 1135 681 219 191 104 76
SfoI GGC'GCC
Cuts at: 2764 2764
Size: 5584
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5584
SmaI CCC'GGG
Cuts at: 1872 2459 2911 1872
Size: 587 452 4545
Fragments arranged by size:
4545 587 452
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3688 4589 4851 820
Size: 197 926 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3375 493
Size: 2882 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5584
SphI G_CATG'C
Cuts at: 1132 2143 2942 1132
Size: 1011 799 3774
Fragments arranged by size:
3774 1011 799
SspI AAT'ATT
Cuts at: 5 52 3508 5
Size: 47 3456 2081
Fragments arranged by size:
3456 2081 47
StuI AGG'CCT
Cuts at: 1517 2738 1517
Size: 1221 4363
StyI C'CwwG_G
Cuts at: 513 1065 2086 2316 3023 513
Size: 552 1021 230 707 3074
Fragments arranged by size:
3074 1021 707 552 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3611 3628 3781 3966 4411 325
Size: 1255 2031 17 153 185 445 1498
Fragments arranged by size:
2031 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3830 5542 96
Size: 274 80 33 51 1443 1853 1712 138
Fragments arranged by size:
1853 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5544 5576 5544
Size: 32 5552
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5584
XbaI T'CTAG_A
Cuts at: 2386 2920 2386
Size: 534 5050
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2084 2084
Size: 5584
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5584
XmaI C'CCGG_G
Cuts at: 1870 2457 2909 1870
Size: 587 452 4545
Fragments arranged by size:
4545 587 452
XmnI GAAnn'nnTTC
Cuts at: 3713 3713
Size: 5584
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3392 276
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI