Restriction map for
pFN21AE3518
(Circular) (Six-base) MAPSORT of: pFN21AE3518.seq Check: 6975 from: 1 to: 6778
pFN21AE3518 6778 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2034 4588 278
Size: 53 83 186 936 498 2554 2468
Fragments arranged by size:
2554 2468 936 498 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4121 1937
Size: 2184 4594
Acc65I G'GTAC_C
Cuts at: 4099 4099
Size: 6778
AclI AA'CG_TT
Cuts at: 1563 2947 2973 4905 5278 1563
Size: 1384 26 1932 373 3063
Fragments arranged by size:
3063 1932 1384 373 26
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2040 2312 2556 2916 3477 4841 6225 6493
Size: 408 272 244 360 561 1364 1384 268
Cuts at: 6493 1632
Size: 1917
Fragments arranged by size:
1917 1384 1364 561 408 360 272 268
244
AflII C'TTAA_G
Cuts at: 820 1017 2444 820
Size: 197 1427 5154
Fragments arranged by size:
5154 1427 197
AflIII A'CryG_T
Cuts at: 1203 3796 5677 6654 1203
Size: 2593 1881 977 1327
Fragments arranged by size:
2593 1881 1327 977
AgeI A'CCGG_T
Cuts at: 5609 5609
Size: 6778
AhdI GACnn_n'nnGTC
Cuts at: 3130 5507 3130
Size: 2377 4401
AleI CACnn'nnGTG
Cuts at: 1166 3197 1166
Size: 2031 4747
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2494 3633 1184
Size: 1310 1139 4329
Fragments arranged by size:
4329 1310 1139
AlwNI CAG_nnn'CTG
Cuts at: 3116 4521 6093 3116
Size: 1405 1572 3801
Fragments arranged by size:
3801 1572 1405
ApaLI G'TGCA_C
Cuts at: 4835 5991 4835
Size: 1156 5622
ApoI r'AATT_y
Cuts at: 1493 3231 3421 4036 4087 4313 4465 6574 1493
Size: 1738 190 615 51 226 152 2109 1697
Fragments arranged by size:
2109 1738 1697 615 226 190 152 51
AseI AT'TA_AT
Cuts at: 160 1032 5332 160
Size: 872 4300 1606
Fragments arranged by size:
4300 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6778
AvaI C'yCGr_G
Cuts at: 1870 1943 4103 1870
Size: 73 2160 4545
Fragments arranged by size:
4545 2160 73
AvrII C'CTAG_G
Cuts at: 3740 3740
Size: 6778
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2982 3015 2982
Size: 33 6745
BamHI G'GATC_C
Cuts at: 4108 4475 4108
Size: 367 6411
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 4099 5554 618
Size: 325 217 627 221 2091 1455 1842
Fragments arranged by size:
2091 1842 1455 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2076 3226 4097 1391
Size: 685 1150 871 4072
Fragments arranged by size:
4072 1150 871 685
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 6778
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2026 3149 928
Size: 971 127 1123 4557
Fragments arranged by size:
4557 1123 971 127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2108 2142 4044 4078 4969 5003 1903
Size: 34 171 34 1902 34 891 34 3678
Fragments arranged by size:
3678 1902 891 171 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2485 4670 5880 2485
Size: 2185 1210 3383
Fragments arranged by size:
3383 2185 1210
BclI T'GATC_A
Cuts at: 1548 3781 1548
Size: 2233 4545
BfrBI ATG'CAT
Cuts at: 2173 2173
Size: 6778
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4071 5389 136
Size: 107 122 71 631 605 2399 1318 1525
Fragments arranged by size:
2399 1525 1318 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6773 6773
Size: 6778
BlpI GC'TnA_GC
Cuts at: 2270 4195 2270
Size: 1925 4853
Bme1580I G_kGCm'C
Cuts at: 1790 4839 5995 1790
Size: 3049 1156 2573
Fragments arranged by size:
3049 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3797 1318
Size: 2479 4299
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4509 5467 442
Size: 416 53 83 399 24 3092 958 1753
Fragments arranged by size:
3092 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6778
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3098 3609 5438 1239
Size: 1859 511 1829 2579
Fragments arranged by size:
2579 1859 1829 511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2655 4903 5768 6066 2655
Size: 2248 865 298 3367
Fragments arranged by size:
3367 2248 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2715 3854 5441 882
Size: 617 1216 1139 1587 2219
Fragments arranged by size:
2219 1587 1216 1139 617
BsaAI yAC'GTr
Cuts at: 493 1204 4569 493
Size: 711 3365 2702
Fragments arranged by size:
3365 2702 711
BsaBI GATnn'nnATC
Cuts at: 6772 6772
Size: 6778
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2031 4585 4967
Size: 53 83 186 936 476 22 2554 382
Cuts at: 4967 5602 275
Size: 635 1451
Fragments arranged by size:
2554 1451 936 635 476 382 186 83
53 22
BsaWI w'CCGG_w
Cuts at: 5211 5609 5883 6030 6441 5211
Size: 398 274 147 411 5548
Fragments arranged by size:
5548 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2937 2967 1178
Size: 30 165 30 1534 30 4989
Fragments arranged by size:
4989 1534 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2411 3885 1186
Size: 1225 1474 4079
Fragments arranged by size:
4079 1474 1225
BseYI C'CCAG_C
Cuts at: 5981 6477 5981
Size: 496 6282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3948 1163
Size: 573 2212 3993
Fragments arranged by size:
3993 2212 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4228 4989 6017 1540
Size: 275 186 2227 761 1028 2301
Fragments arranged by size:
2301 2227 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2131 3226 3894 4839 4924 5995 2131
Size: 1095 668 945 85 1071 2914
Fragments arranged by size:
2914 1095 1071 945 668 85
BsmI GAATG_Cn'
Cuts at: 4289 4382 4289
Size: 93 6685
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2131 3226 3894 4097 4839 4924
Size: 399 286 55 1095 668 203 742 85
Cuts at: 4924 5995 1391
Size: 1071 2174
Fragments arranged by size:
2174 1095 1071 742 668 399 286 203
85 55
BspEI T'CCGG_A
Cuts at: 6441 6441
Size: 6778
BspHI T'CATG_A
Cuts at: 2860 4667 6397 2860
Size: 1807 1730 3241
Fragments arranged by size:
3241 1807 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4133 844
Size: 3289 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4068 4665 1394
Size: 429 2245 597 3507
Fragments arranged by size:
3507 2245 597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2928 3714 5273 5447 65
Size: 1183 1680 786 1559 174 1396
Fragments arranged by size:
1680 1559 1396 1183 786 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5422 5609 1700
Size: 110 3612 187 2869
Fragments arranged by size:
3612 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2239 3659 96
Size: 2143 1420 3215
Fragments arranged by size:
3215 2143 1420
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6778
BssSI C'ACGA_G
Cuts at: 4838 5850 4838
Size: 1012 5766
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6778
BstBI TT'CG_AA
Cuts at: 5582 5582
Size: 6778
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6778
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 3043 2419
Size: 624 6154
BstYI r'GATC_y
Cuts at: 1972 3088 4108 4475 4860 4877 6329 6415 6427
Size: 1116 1020 367 385 17 1452 86 12
Cuts at: 6427 6773 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1116 1020 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2634 2634
Size: 6778
BtgI C'CryG_G
Cuts at: 513 1065 2004 2565 2956 3844 513
Size: 552 939 561 391 888 3447
Fragments arranged by size:
3447 939 888 561 552 391
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6039
Fragments arranged by size:
6039 683 56
BtsI GCAGTG_nn'
Cuts at: 2476 4049 4294 5088 5108 2476
Size: 1573 245 794 20 4146
Fragments arranged by size:
4146 1573 794 245 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6778
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6743
DraI TTT'AAA
Cuts at: 2557 4082 2557
Size: 1525 5253
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5785 809
Size: 507 4469 1802
Fragments arranged by size:
4469 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4225 5114 6743
Size: 54 1425 50 275 18 2395 889 1629
Cuts at: 6743 8
Size: 43
Fragments arranged by size:
2395 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4225 1537
Size: 275 2413 4090
Fragments arranged by size:
4090 2413 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2136 2388 2433 2532 2892 4708 1353
Size: 783 252 45 99 360 1816 3423
Fragments arranged by size:
3423 1816 783 360 252 99 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2068 5365 5739 5885 387
Size: 1064 138 479 3297 374 146 1280
Fragments arranged by size:
3297 1280 1064 479 374 146 138
EcoICRI GAG'CTC
Cuts at: 3224 3224
Size: 6778
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2312 2556 2916 3098 3477 3609
Size: 393 408 272 244 360 182 379 132
Cuts at: 3609 4841 5438 6225 6493 1239
Size: 1232 597 787 268 1524
Fragments arranged by size:
1524 1232 787 597 408 393 379 360
272 268 244 182 132
EcoRI G'AATT_C
Cuts at: 3231 4087 4465 3231
Size: 856 378 5544
Fragments arranged by size:
5544 856 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2871 2871
Size: 6778
FspI TGC'GCA
Cuts at: 5284 5284
Size: 6778
HaeII r_GCGC'y
Cuts at: 1678 2012 5925 1678
Size: 334 3913 2531
Fragments arranged by size:
3913 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2561 2593 3116 3148 3229
Size: 32 135 32 989 32 523 32 81
Cuts at: 3229 3261 5419 5451 5493 5525 1373
Size: 32 2158 32 42 32 2626
Fragments arranged by size:
2626 2158 989 523 135 81 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4122 4368 669
Size: 988 281 2184 246 3079
Fragments arranged by size:
3079 2184 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2087 748
Size: 1339 5439
HpaI GTT'AAC
Cuts at: 4368 4368
Size: 6778
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 6778
KpnI G_GTAC'C
Cuts at: 4103 4103
Size: 6778
MfeI C'AATT_G
Cuts at: 2916 4377 2916
Size: 1461 5317
MluI A'CGCG_T
Cuts at: 6654 6654
Size: 6778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2133 3561 3562 5892 6076 1305
Size: 828 1428 1 2330 184 2007
Fragments arranged by size:
2330 2007 1428 828 184 1
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4956
Fragments arranged by size:
4956 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 3197 3249 3314 3501 4736 5095
Size: 648 1251 780 52 65 187 1235 359
Cuts at: 5095 5254 518
Size: 159 2042
Fragments arranged by size:
2042 1251 1235 780 648 359 187 159
65 52
MspA1I CmG'CkG
Cuts at: 1606 2065 2716 4194 4871 6019 6264 1606
Size: 459 651 1478 677 1148 245 2120
Fragments arranged by size:
2120 1478 1148 677 651 459 245
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 6778
NcoI C'CATG_G
Cuts at: 513 1065 2004 2956 3844 513
Size: 552 939 952 888 3447
Fragments arranged by size:
3447 952 939 888 552
NdeI CA'TA_TG
Cuts at: 387 2983 387
Size: 2596 4182
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6778
NotI GC'GGCC_GC
Cuts at: 4225 4225
Size: 6778
NsiI A_TGCA'T
Cuts at: 2175 2175
Size: 6778
NspI r_CATG'y
Cuts at: 1132 1229 3957 4136 5681 1132
Size: 97 2728 179 1545 2229
Fragments arranged by size:
2728 2229 1545 179 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6778
PciI A'CATG_T
Cuts at: 5677 5677
Size: 6778
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2987 1108
Size: 1879 4899
PmeI GTTT'AAAC
Cuts at: 4082 4082
Size: 6778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4847 4879 6360 6392 4847
Size: 32 1481 32 5233
Fragments arranged by size:
5233 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3596 3846 1613
Size: 1983 250 4545
Fragments arranged by size:
4545 1983 250
PsiI TTA'TAA
Cuts at: 2235 4348 2235
Size: 2113 4665
PstI C_TGCA'G
Cuts at: 830 2139 2500 3031 3939 4130 830
Size: 1309 361 531 908 191 3478
Fragments arranged by size:
3478 1309 908 531 361 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6778
PvuII CAG'CTG
Cuts at: 2065 2716 2065
Size: 651 6127
SacI G_AGCT'C
Cuts at: 3226 3226
Size: 6778
SalI G'TCGA_C
Cuts at: 1936 4120 1936
Size: 2184 4594
SapI GCTCTTCn'nnn_
Cuts at: 2136 2433 2532 2136
Size: 297 99 6382
Fragments arranged by size:
6382 297 99
SbfI CC_TGCA'GG
Cuts at: 3939 4130 3939
Size: 191 6587
ScaI AGT'ACT
Cuts at: 5026 6738 5026
Size: 1712 5066
SfcI C'TryA_G
Cuts at: 826 1045 2135 2496 3027 3935 4126 5261 5942
Size: 219 1090 361 531 908 191 1135 681
Cuts at: 5942 6133 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1090 908 681 531 361 219
191 191
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 6778
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6778
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6778
SmaI CCC'GGG
Cuts at: 1872 4105 1872
Size: 2233 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2444 2670 4882 5783 6045 820
Size: 197 926 501 226 2212 901 262 1553
Fragments arranged by size:
2212 1553 926 901 501 262 226 197
SnaBI TAC'GTA
Cuts at: 493 4569 493
Size: 4076 2702
SpeI A'CTAG_T
Cuts at: 152 3593 152
Size: 3441 3337
SphI G_CATG'C
Cuts at: 1132 4136 1132
Size: 3004 3774
SspI AAT'ATT
Cuts at: 5 52 2426 2561 4702 5
Size: 47 2374 135 2141 2081
Fragments arranged by size:
2374 2141 2081 135 47
StuI AGG'CCT
Cuts at: 1517 3933 1517
Size: 2416 4362
StyI C'CwwG_G
Cuts at: 513 1065 2004 2956 3740 3844 4217 513
Size: 552 939 952 784 104 373 3074
Fragments arranged by size:
3074 952 939 784 552 373 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2815 4805 4822 4975 5160 5605 325
Size: 1255 1235 1990 17 153 185 445 1498
Fragments arranged by size:
1990 1498 1255 1235 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2239 3659 3799
Size: 274 80 33 51 1443 262 1420 140
Cuts at: 3799 5024 6736 96
Size: 1225 1712 138
Fragments arranged by size:
1712 1443 1420 1225 274 262 140 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6738 6770 6738
Size: 32 6746
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6778
XbaI T'CTAG_A
Cuts at: 4114 4114
Size: 6778
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6778
XmaI C'CCGG_G
Cuts at: 1870 4103 1870
Size: 2233 4545
XmnI GAAnn'nnTTC
Cuts at: 4907 4907
Size: 6778
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2032 4586 276
Size: 53 83 186 936 498 2554 2468
Fragments arranged by size:
2554 2468 936 498 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbvCI BplI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI
FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SrfI SwaI XcmI