Restriction map for
pFN21AE3512
(Circular) (Six-base) MAPSORT of: pFN21AE3512.seq Check: 8258 from: 1 to: 6541
pFN21AE3512 6541 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4351 278
Size: 53 83 186 936 2815 2468
Fragments arranged by size:
2815 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3309 3884 1937
Size: 1372 575 4594
Fragments arranged by size:
4594 1372 575
Acc65I G'GTAC_C
Cuts at: 3862 3862
Size: 6541
AclI AA'CG_TT
Cuts at: 1563 4668 5041 1563
Size: 3105 373 3063
Fragments arranged by size:
3105 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2668 2752 3424 4604 5988 6256 1632
Size: 1036 84 672 1180 1384 268 1917
Fragments arranged by size:
1917 1384 1180 1036 672 268 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6344
AflIII A'CryG_T
Cuts at: 1203 5440 6417 1203
Size: 4237 977 1327
Fragments arranged by size:
4237 1327 977
AgeI A'CCGG_T
Cuts at: 5372 5372
Size: 6541
AhdI GACnn_n'nnGTC
Cuts at: 5270 5270
Size: 6541
AleI CACnn'nnGTG
Cuts at: 1166 2811 3399 3483 1166
Size: 1645 588 84 4224
Fragments arranged by size:
4224 1645 588 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6541
AlwNI CAG_nnn'CTG
Cuts at: 4284 5856 4284
Size: 1572 4969
ApaLI G'TGCA_C
Cuts at: 4598 5754 4598
Size: 1156 5385
ApoI r'AATT_y
Cuts at: 1493 2550 2860 2886 2970 3138 3222 3850 4076
Size: 1057 310 26 84 168 84 628 226
Cuts at: 4076 4228 6337 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1057 628 310 226 168 152
84 84 26
AseI AT'TA_AT
Cuts at: 160 1032 5095 160
Size: 872 4063 1606
Fragments arranged by size:
4063 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 3619 3866 1870
Size: 73 71 1605 247 4545
Fragments arranged by size:
4545 1605 247 73 71
BamHI G'GATC_C
Cuts at: 2242 3054 3871 4238 2242
Size: 812 817 367 4545
Fragments arranged by size:
4545 817 812 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3862 5317 618
Size: 325 217 627 2075 1455 1842
Fragments arranged by size:
2075 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2021 2612 3032 3452 3860 1391
Size: 630 591 420 420 408 4072
Fragments arranged by size:
4072 630 591 420 420 408
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3245 928
Size: 971 1346 4224
Fragments arranged by size:
4224 1346 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4732 4766 1903
Size: 34 2795 34 3678
Fragments arranged by size:
3678 2795 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4433 5643 4433
Size: 1210 5331
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6541
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5152 136
Size: 107 122 71 631 605 3480 1525
Fragments arranged by size:
3480 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6536 6536
Size: 6541
BlpI GC'TnA_GC
Cuts at: 3958 3958
Size: 6541
Bme1580I G_kGCm'C
Cuts at: 1790 4602 5758 1790
Size: 2812 1156 2573
Fragments arranged by size:
2812 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6541
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2568 2904 4272
Size: 416 53 83 399 24 1151 336 1368
Cuts at: 4272 5230 442
Size: 958 1753
Fragments arranged by size:
1753 1368 1151 958 416 399 336 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2664 5201 1239
Size: 1425 2537 2579
Fragments arranged by size:
2579 2537 1425
Bpu10I CC'TnA_GC
Cuts at: 3199 3199
Size: 6541
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4666 5531 5829 4666
Size: 865 298 5378
Fragments arranged by size:
5378 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2119 3484 5204 882
Size: 617 620 1365 1720 2219
Fragments arranged by size:
2219 1720 1365 620 617
BsaAI yAC'GTr
Cuts at: 493 1204 4332 493
Size: 711 3128 2702
Fragments arranged by size:
3128 2702 711
BsaBI GATnn'nnATC
Cuts at: 6535 6535
Size: 6541
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4348 4730 5365 275
Size: 53 83 186 936 2815 382 635 1451
Fragments arranged by size:
2815 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4974 5372 5646 5793 6204 4974
Size: 398 274 147 411 5311
Fragments arranged by size:
5311 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6316
Fragments arranged by size:
6316 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2162 1186
Size: 868 108 5565
Fragments arranged by size:
5565 868 108
BseYI C'CCAG_C
Cuts at: 2059 5744 6240 2059
Size: 3685 496 2360
Fragments arranged by size:
3685 2360 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3523 1163
Size: 573 1787 4181
Fragments arranged by size:
4181 1787 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3991 4752 5780 1540
Size: 275 186 1990 761 1028 2301
Fragments arranged by size:
2301 1990 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2612 3032 3370 3452 4602 4687 5758 2612
Size: 420 338 82 1150 85 1071 3395
Fragments arranged by size:
3395 1150 1071 420 338 85 82
BsmI GAATG_Cn'
Cuts at: 4052 4145 4052
Size: 93 6448
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2612 3032 3370 3452 3860 4602
Size: 399 231 591 420 338 82 408 742
Cuts at: 4602 4687 5758 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 591 420 408 399 338
231 85 82
BspEI T'CCGG_A
Cuts at: 6204 6204
Size: 6541
BspHI T'CATG_A
Cuts at: 2088 4430 6160 2088
Size: 2342 1730 2469
Fragments arranged by size:
2469 2342 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3896 844
Size: 3052 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4428 1394
Size: 429 2605 3507
Fragments arranged by size:
3507 2605 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2503 2864 5036 5210 65
Size: 1183 1255 361 2172 174 1396
Fragments arranged by size:
2172 1396 1255 1183 361 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5185 5372 1700
Size: 110 3375 187 2869
Fragments arranged by size:
3375 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3376 96
Size: 3280 3261
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6541
BssSI C'ACGA_G
Cuts at: 4601 5613 4601
Size: 1012 5529
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6541
BstBI TT'CG_AA
Cuts at: 5345 5345
Size: 6541
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6541
BstXI CCAn_nnnn'nTGG
Cuts at: 2915 2915
Size: 6541
BstYI r'GATC_y
Cuts at: 1972 2242 2298 3054 3726 3871 4238 4623 4640
Size: 270 56 756 672 145 367 385 17
Cuts at: 4640 6092 6178 6190 6536 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 756 672 385 367 346 270
145 86 56 17 12
Bsu36I CC'TnA_GG
Cuts at: 3315 3315
Size: 6541
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5050
Fragments arranged by size:
5050 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5802
Fragments arranged by size:
5802 683 56
BtsI GCAGTG_nn'
Cuts at: 4057 4851 4871 4057
Size: 794 20 5727
Fragments arranged by size:
5727 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6541
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2485 2520 3073 3108 529
Size: 35 1921 35 553 35 3962
Fragments arranged by size:
3962 1921 553 35 35 35
DraI TTT'AAA
Cuts at: 3845 3845
Size: 6541
DraIII CAC_nnn'GTG
Cuts at: 2204 2204
Size: 6541
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5548 809
Size: 507 4232 1802
Fragments arranged by size:
4232 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3988 4877 6506
Size: 54 1425 50 275 18 2158 889 1629
Cuts at: 6506 8
Size: 43
Fragments arranged by size:
2158 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3988 1537
Size: 275 2176 4090
Fragments arranged by size:
4090 2176 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2388 2840 4471 1353
Size: 1035 452 1631 3423
Fragments arranged by size:
3423 1631 1035 452
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5128 5502 5648 387
Size: 1064 138 3539 374 146 1280
Fragments arranged by size:
3539 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2610 3030 3450 2610
Size: 420 420 5701
Fragments arranged by size:
5701 420 420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2664 2668 2752 3424 4604 5201 5988
Size: 393 1032 4 84 672 1180 597 787
Cuts at: 5988 6256 1239
Size: 268 1524
Fragments arranged by size:
1524 1180 1032 787 672 597 393 268
84 4
EcoO109I rG'GnC_Cy
Cuts at: 2568 2568
Size: 6541
EcoRI G'AATT_C
Cuts at: 2550 2886 3138 3222 3850 4228 2550
Size: 336 252 84 628 378 4863
Fragments arranged by size:
4863 628 378 336 252 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2343 2866 2343
Size: 523 6018
FspI TGC'GCA
Cuts at: 5047 5047
Size: 6541
HaeII r_GCGC'y
Cuts at: 1678 5688 1678
Size: 4010 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2839 2871 3481 3513 5182
Size: 32 135 32 1267 32 610 32 1669
Cuts at: 5182 5214 5256 5288 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1669 1267 610 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3698 3715 3885 4131 669
Size: 988 281 1760 17 170 246 3079
Fragments arranged by size:
3079 1760 988 281 246 170 17
HindIII A'AGCT_T
Cuts at: 748 3436 3520 748
Size: 2688 84 3769
Fragments arranged by size:
3769 2688 84
HpaI GTT'AAC
Cuts at: 4131 4131
Size: 6541
KpnI G_GTAC'C
Cuts at: 3866 3866
Size: 6541
MfeI C'AATT_G
Cuts at: 4140 4140
Size: 6541
MluI A'CGCG_T
Cuts at: 6417 6417
Size: 6541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2753 3005 5655 5839 1305
Size: 1448 252 2650 184 2007
Fragments arranged by size:
2650 2007 1448 252 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4719
Fragments arranged by size:
4719 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2409 2475 2727 2811 2913 3063 3165
Size: 648 1243 66 252 84 102 150 102
Cuts at: 3165 3399 3483 3555 3585 3762 4499 4858 5017
Size: 234 84 72 30 177 737 359 159
Cuts at: 5017 518
Size: 2042
Fragments arranged by size:
2042 1243 737 648 359 252 234 177
159 150 102 102 84 84 72 66
30
MspA1I CmG'CkG
Cuts at: 1606 3957 4634 5782 6027 1606
Size: 2351 677 1148 245 2120
Fragments arranged by size:
2351 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5050
Fragments arranged by size:
5050 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6541
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6541
NotI GC'GGCC_GC
Cuts at: 3988 3988
Size: 6541
NspI r_CATG'y
Cuts at: 1132 1229 3899 5444 1132
Size: 97 2670 1545 2229
Fragments arranged by size:
2670 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6541
PciI A'CATG_T
Cuts at: 5440 5440
Size: 6541
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6541
PmeI GTTT'AAAC
Cuts at: 3845 3845
Size: 6541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4610 4642 6123 6155 4610
Size: 32 1481 32 4996
Fragments arranged by size:
4996 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6541
PsiI TTA'TAA
Cuts at: 3330 3666 4111 3330
Size: 336 445 5760
Fragments arranged by size:
5760 445 336
PstI C_TGCA'G
Cuts at: 830 3893 830
Size: 3063 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6541
SacI G_AGCT'C
Cuts at: 2612 3032 3452 2612
Size: 420 420 5701
Fragments arranged by size:
5701 420 420
SalI G'TCGA_C
Cuts at: 1936 3883 1936
Size: 1947 4594
SapI GCTCTTCn'nnn_
Cuts at: 2840 2840
Size: 6541
SbfI CC_TGCA'GG
Cuts at: 3893 3893
Size: 6541
ScaI AGT'ACT
Cuts at: 4789 6501 4789
Size: 1712 4829
SexAI A'CCwGG_T
Cuts at: 2109 2109
Size: 6541
SfcI C'TryA_G
Cuts at: 826 1045 3889 5024 5705 5896 826
Size: 219 2844 1135 681 191 1471
Fragments arranged by size:
2844 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6541
SmaI CCC'GGG
Cuts at: 1872 3868 1872
Size: 1996 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4645 5546 5808 820
Size: 197 926 2702 901 262 1553
Fragments arranged by size:
2702 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4332 493
Size: 3839 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6541
SphI G_CATG'C
Cuts at: 1132 3899 1132
Size: 2767 3774
SspI AAT'ATT
Cuts at: 5 52 4465 5
Size: 47 4413 2081
Fragments arranged by size:
4413 2081 47
StuI AGG'CCT
Cuts at: 1517 3605 1517
Size: 2088 4453
StyI C'CwwG_G
Cuts at: 513 1065 2004 2318 3980 513
Size: 552 939 314 1662 3074
Fragments arranged by size:
3074 1662 939 552 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4568 4585 4738 4923 5368 325
Size: 1255 2988 17 153 185 445 1498
Fragments arranged by size:
2988 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3376 3419 4787
Size: 274 80 33 51 1443 1399 43 1368
Cuts at: 4787 6499 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1399 1368 274 138 80 51
43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6501 6533 6501
Size: 32 6509
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6541
XbaI T'CTAG_A
Cuts at: 3877 3877
Size: 6541
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6541
XmaI C'CCGG_G
Cuts at: 1870 3866 1870
Size: 1996 4545
XmnI GAAnn'nnTTC
Cuts at: 2765 3101 3437 4670 2765
Size: 336 336 1233 4636
Fragments arranged by size:
4636 1233 336 336
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4349 276
Size: 53 83 186 936 2815 2468
Fragments arranged by size:
2815 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI