Restriction map for
pFN21AE3511
(Circular) (Six-base) MAPSORT of: pFN21AE3511.seq Check: 6721 from: 1 to: 5896
pFN21AE3511 5896 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3706 278
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3239 1937
Size: 1302 4594
Acc65I G'GTAC_C
Cuts at: 3217 3217
Size: 5896
AclI AA'CG_TT
Cuts at: 1563 2653 4023 4396 1563
Size: 1090 1370 373 3063
Fragments arranged by size:
3063 1370 1090 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2418 2466 2868 3959 5343 5611 1632
Size: 786 48 402 1091 1384 268 1917
Fragments arranged by size:
1917 1384 1091 786 402 268 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5699
AflIII A'CryG_T
Cuts at: 1203 4795 5772 1203
Size: 3592 977 1327
Fragments arranged by size:
3592 1327 977
AgeI A'CCGG_T
Cuts at: 4727 4727
Size: 5896
AhdI GACnn_n'nnGTC
Cuts at: 4625 4625
Size: 5896
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5896
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5896
AlwNI CAG_nnn'CTG
Cuts at: 2192 2614 2663 3639 5211 2192
Size: 422 49 976 1572 2877
Fragments arranged by size:
2877 1572 976 422 49
ApaLI G'TGCA_C
Cuts at: 3953 5109 3953
Size: 1156 4740
ApoI r'AATT_y
Cuts at: 1493 3205 3431 3583 5692 1493
Size: 1712 226 152 2109 1697
Fragments arranged by size:
2109 1712 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4450 160
Size: 872 3418 1606
Fragments arranged by size:
3418 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
AvaI C'yCGr_G
Cuts at: 1870 1943 3221 1870
Size: 73 1278 4545
Fragments arranged by size:
4545 1278 73
BamHI G'GATC_C
Cuts at: 2537 3226 3593 2537
Size: 689 367 4840
Fragments arranged by size:
4840 689 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3217 4672 618
Size: 325 217 627 1430 1455 1842
Fragments arranged by size:
1842 1455 1430 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2016 2318 3215 1391
Size: 625 302 897 4072
Fragments arranged by size:
4072 897 625 302
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3057 928
Size: 971 1158 3767
Fragments arranged by size:
3767 1158 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4087 4121 1903
Size: 34 2150 34 3678
Fragments arranged by size:
3678 2150 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3788 4998 3788
Size: 1210 4686
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5896
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2108 4507 136
Size: 107 122 71 631 605 436 2399 1525
Fragments arranged by size:
2399 1525 631 605 436 122 107 71
BglII A'GATC_T
Cuts at: 5891 5891
Size: 5896
BlpI GC'TnA_GC
Cuts at: 3313 3313
Size: 5896
Bme1580I G_kGCm'C
Cuts at: 1790 3957 5113 1790
Size: 2167 1156 2573
Fragments arranged by size:
2573 2167 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5896
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2221 3627 4585
Size: 416 53 83 399 24 804 1406 958
Cuts at: 4585 442
Size: 1753
Fragments arranged by size:
1753 1406 958 804 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5896
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2054 2054
Size: 5896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2000 3015 3188 4556 1239
Size: 761 1015 173 1368 2579
Fragments arranged by size:
2579 1368 1015 761 173
Bpu10I CC'TnA_GC
Cuts at: 2084 2084
Size: 5896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2527 4021 4886 5184 2527
Size: 1494 865 298 3239
Fragments arranged by size:
3239 1494 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3164 4559 882
Size: 617 1665 1395 2219
Fragments arranged by size:
2219 1665 1395 617
BsaAI yAC'GTr
Cuts at: 493 1204 3687 493
Size: 711 2483 2702
Fragments arranged by size:
2702 2483 711
BsaBI GATnn'nnATC
Cuts at: 5890 5890
Size: 5896
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3703 4085 4720 275
Size: 53 83 186 936 2170 382 635 1451
Fragments arranged by size:
2170 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4329 4727 5001 5148 5559 4329
Size: 398 274 147 411 4666
Fragments arranged by size:
4666 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2442 2472 2514 2544 1178
Size: 30 165 30 1039 30 42 30 4530
Fragments arranged by size:
4530 1039 165 42 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2498 1186
Size: 838 474 4584
Fragments arranged by size:
4584 838 474
BseYI C'CCAG_C
Cuts at: 2362 2995 5099 5595 2362
Size: 633 2104 496 2663
Fragments arranged by size:
2663 2104 633 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2913 1163
Size: 573 1177 4146
Fragments arranged by size:
4146 1177 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2144 3346 4107 5135 1540
Size: 275 186 143 1202 761 1028 2301
Fragments arranged by size:
2301 1202 1028 761 275 186 143
BsiHKAI G_wGCw'C
Cuts at: 2016 2553 3957 4042 5113 2016
Size: 537 1404 85 1071 2799
Fragments arranged by size:
2799 1404 1071 537 85
BsmI GAATG_Cn'
Cuts at: 3407 3500 3407
Size: 93 5803
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2318 2553 3215 3957 4042 5113
Size: 399 226 302 235 662 742 85 1071
Cuts at: 5113 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 662 399 302 235 226
85
BspEI T'CCGG_A
Cuts at: 5559 5559
Size: 5896
BspHI T'CATG_A
Cuts at: 3785 5515 3785
Size: 1730 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3251 844
Size: 2407 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3783 1394
Size: 429 1960 3507
Fragments arranged by size:
3507 1960 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4391 4565 65
Size: 1183 3143 174 1396
Fragments arranged by size:
3143 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2143 4540 4727 1700
Size: 110 333 2397 187 2869
Fragments arranged by size:
2869 2397 333 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5896
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5896
BssSI C'ACGA_G
Cuts at: 3956 4968 3956
Size: 1012 4884
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5896
BstBI TT'CG_AA
Cuts at: 4700 4700
Size: 5896
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5896
BstYI r'GATC_y
Cuts at: 1972 2500 2537 3226 3593 3978 3995 5447 5533
Size: 528 37 689 367 385 17 1452 86
Cuts at: 5533 5545 5891 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 689 528 385 367 346 86
37 17 12
Bsu36I CC'TnA_GG
Cuts at: 2282 2282
Size: 5896
BtgI C'CryG_G
Cuts at: 513 1065 2004 2349 513
Size: 552 939 345 4060
Fragments arranged by size:
4060 939 552 345
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5157
Fragments arranged by size:
5157 683 56
BtsI GCAGTG_nn'
Cuts at: 2305 2612 3412 4206 4226 2305
Size: 307 800 794 20 3975
Fragments arranged by size:
3975 800 794 307 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5896
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5861
DraI TTT'AAA
Cuts at: 3200 3200
Size: 5896
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4903 809
Size: 507 3587 1802
Fragments arranged by size:
3587 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2141 3343 4232
Size: 54 1425 50 275 18 311 1202 889
Cuts at: 4232 5861 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1202 889 311 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2141 3343 1537
Size: 275 329 1202 4090
Fragments arranged by size:
4090 1202 329 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2213 3826 1353
Size: 860 1613 3423
Fragments arranged by size:
3423 1613 860
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4483 4857 5003 387
Size: 1064 138 2894 374 146 1280
Fragments arranged by size:
2894 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2014 2014
Size: 5896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2000 2418 2466 2868 3015 3188 3959
Size: 393 368 418 48 402 147 173 771
Cuts at: 3959 4556 5343 5611 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 771 597 418 402 393 368
268 173 147 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2028 2028
Size: 5896
EcoO109I rG'GnC_Cy
Cuts at: 2189 3043 2189
Size: 854 5042
EcoRI G'AATT_C
Cuts at: 3205 3583 3205
Size: 378 5518
FspI TGC'GCA
Cuts at: 4402 4402
Size: 5896
HaeII r_GCGC'y
Cuts at: 1678 2114 2247 2332 5043 1678
Size: 436 133 85 2711 2531
Fragments arranged by size:
2711 2531 436 133 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2474 2476 2506 2514
Size: 32 135 32 872 30 2 30 8
Cuts at: 2514 2546 2819 2851 4537 4569 4611 4643 1373
Size: 32 273 32 1686 32 42 32 2626
Fragments arranged by size:
2626 1686 872 273 135 42 32 32
32 32 32 32 30 30 8 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3240 3486 669
Size: 988 281 1302 246 3079
Fragments arranged by size:
3079 1302 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5896
HpaI GTT'AAC
Cuts at: 3486 3486
Size: 5896
KpnI G_GTAC'C
Cuts at: 3221 3221
Size: 5896
MfeI C'AATT_G
Cuts at: 3495 3495
Size: 5896
MluI A'CGCG_T
Cuts at: 5772 5772
Size: 5896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5010 5194 1305
Size: 3705 184 2007
Fragments arranged by size:
3705 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4074
Fragments arranged by size:
4074 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2910 2961 3854 4213 4372 518
Size: 648 1744 51 893 359 159 2042
Fragments arranged by size:
2042 1744 893 648 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 2128 3312 3989 5137 5382 1606
Size: 522 1184 677 1148 245 2120
Fragments arranged by size:
2120 1184 1148 677 522 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2349 513
Size: 552 939 345 4060
Fragments arranged by size:
4060 939 552 345
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5896
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5896
NotI GC'GGCC_GC
Cuts at: 3343 3343
Size: 5896
NspI r_CATG'y
Cuts at: 1132 1229 2909 2960 3254 4799 1132
Size: 97 1680 51 294 1545 2229
Fragments arranged by size:
2229 1680 1545 294 97 51
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5896
PciI A'CATG_T
Cuts at: 4795 4795
Size: 5896
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2192 2311 1108
Size: 1084 119 4693
Fragments arranged by size:
4693 1084 119
PfoI T'CCnGG_A
Cuts at: 2016 3045 3063 3165 2016
Size: 1029 18 102 4747
Fragments arranged by size:
4747 1029 102 18
PmeI GTTT'AAAC
Cuts at: 3200 3200
Size: 5896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2589 2621 3965 3997 5478 5510 2589
Size: 32 1344 32 1481 32 2975
Fragments arranged by size:
2975 1481 1344 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3043 3043
Size: 5896
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5896
PsiI TTA'TAA
Cuts at: 3466 3466
Size: 5896
PstI C_TGCA'G
Cuts at: 830 3248 830
Size: 2418 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5896
SacI G_AGCT'C
Cuts at: 2016 2016
Size: 5896
SalI G'TCGA_C
Cuts at: 1936 3238 1936
Size: 1302 4594
SbfI CC_TGCA'GG
Cuts at: 3248 3248
Size: 5896
ScaI AGT'ACT
Cuts at: 4144 5856 4144
Size: 1712 4184
SfcI C'TryA_G
Cuts at: 826 1045 2664 2673 3244 4379 5060 5251 826
Size: 219 1619 9 571 1135 681 191 1471
Fragments arranged by size:
1619 1471 1135 681 571 219 191 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5896
SmaI CCC'GGG
Cuts at: 1872 3223 1872
Size: 1351 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2542 4000 4901 5163 820
Size: 197 926 599 1458 901 262 1553
Fragments arranged by size:
1553 1458 926 901 599 262 197
SnaBI TAC'GTA
Cuts at: 493 3687 493
Size: 3194 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5896
SphI G_CATG'C
Cuts at: 1132 2960 3254 1132
Size: 1828 294 3774
Fragments arranged by size:
3774 1828 294
SspI AAT'ATT
Cuts at: 5 52 3820 5
Size: 47 3768 2081
Fragments arranged by size:
3768 2081 47
StuI AGG'CCT
Cuts at: 1517 2733 1517
Size: 1216 4680
StyI C'CwwG_G
Cuts at: 513 1065 2004 2158 2349 2518 3335 513
Size: 552 939 154 191 169 817 3074
Fragments arranged by size:
3074 939 817 552 191 169 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3923 3940 4093 4278 4723 325
Size: 1255 2343 17 153 185 445 1498
Fragments arranged by size:
2343 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4142 5854 96
Size: 274 80 33 51 1443 2165 1712 138
Fragments arranged by size:
2165 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2252 2284 5856 5888 2252
Size: 32 3572 32 2260
Fragments arranged by size:
3572 2260 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5896
XbaI T'CTAG_A
Cuts at: 3232 3232
Size: 5896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2193 2677 2193
Size: 484 5412
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5896
XmaI C'CCGG_G
Cuts at: 1870 3221 1870
Size: 1351 4545
XmnI GAAnn'nnTTC
Cuts at: 2604 4025 2604
Size: 1421 4475
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3704 276
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI