Restriction map for
pFN21AE3508
(Circular) (Six-base) MAPSORT of: pFN21AE3508.seq Check: 2106 from: 1 to: 6814
pFN21AE3508 6814 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4624 278
Size: 53 83 186 936 3088 2468
Fragments arranged by size:
3088 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4157 1937
Size: 2220 4594
Acc65I G'GTAC_C
Cuts at: 2193 4135 2193
Size: 1942 4872
AclI AA'CG_TT
Cuts at: 1563 4941 5314 1563
Size: 3378 373 3063
Fragments arranged by size:
3378 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3156 3945 4877 6261 6529 1632
Size: 1524 789 932 1384 268 1917
Fragments arranged by size:
1917 1524 1384 932 789 268
AfeI AGC'GCT
Cuts at: 3522 3522
Size: 6814
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6617
AflIII A'CryG_T
Cuts at: 1203 3576 5713 6690 1203
Size: 2373 2137 977 1327
Fragments arranged by size:
2373 2137 1327 977
AgeI A'CCGG_T
Cuts at: 5645 5645
Size: 6814
AhdI GACnn_n'nnGTC
Cuts at: 5543 5543
Size: 6814
AleI CACnn'nnGTG
Cuts at: 1166 3320 1166
Size: 2154 4660
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2484 3416 1184
Size: 1300 932 4582
Fragments arranged by size:
4582 1300 932
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4080 4112 4080
Size: 32 6782
AlwNI CAG_nnn'CTG
Cuts at: 2069 2383 3065 4557 6129 2069
Size: 314 682 1492 1572 2754
Fragments arranged by size:
2754 1572 1492 682 314
ApaI G_GGCC'C
Cuts at: 2933 3133 3821 2933
Size: 200 688 5926
Fragments arranged by size:
5926 688 200
ApaLI G'TGCA_C
Cuts at: 4871 6027 4871
Size: 1156 5658
ApoI r'AATT_y
Cuts at: 1493 4123 4349 4501 6610 1493
Size: 2630 226 152 2109 1697
Fragments arranged by size:
2630 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5368 160
Size: 872 4336 1606
Fragments arranged by size:
4336 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6814
AvaI C'yCGr_G
Cuts at: 1870 1943 2043 2211 2516 2691 4139 1870
Size: 73 100 168 305 175 1448 4545
Fragments arranged by size:
4545 1448 305 175 168 100 73
BamHI G'GATC_C
Cuts at: 4144 4511 4144
Size: 367 6447
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2025 2193 2468 3602 3674
Size: 325 217 627 238 168 275 1134 72
Cuts at: 3674 4016 4135 5590 618
Size: 342 119 1455 1842
Fragments arranged by size:
1842 1455 1134 627 342 325 275 238
217 168 119 72
BanII G_rGCy'C
Cuts at: 1391 2157 2631 2774 2880 2933 3133 3821 3828
Size: 766 474 143 106 53 200 688 7
Cuts at: 3828 3963 4133 1391
Size: 135 170 4072
Fragments arranged by size:
4072 766 688 474 200 170 143 135
106 53 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5843
BbvCI CC'TCA_GC
Cuts at: 2717 2717
Size: 6814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5005 5039 1903
Size: 34 3068 34 3678
Fragments arranged by size:
3678 3068 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3336 4706 5916 3336
Size: 1370 1210 4234
Fragments arranged by size:
4234 1370 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6814
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2577 2586 5425
Size: 107 122 71 631 605 905 9 2839
Cuts at: 5425 136
Size: 1525
Fragments arranged by size:
2839 1525 905 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 6809 6809
Size: 6814
BlpI GC'TnA_GC
Cuts at: 3746 4231 3746
Size: 485 6329
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2415 2716 2902 2933 3133 3607 3821
Size: 240 385 301 186 31 200 474 214
Cuts at: 3821 4875 6031 1790
Size: 1054 1156 2573
Fragments arranged by size:
2573 1156 1054 474 385 301 240 214
200 186 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6814
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3154 3356 4545
Size: 416 53 83 399 24 1737 202 1189
Cuts at: 4545 5503 442
Size: 958 1753
Fragments arranged by size:
1753 1737 1189 958 416 399 202 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2562 1056
Size: 1506 5308
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3817 3817
Size: 6814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2076 3011 3058 3263 3653 3812 3893 3986
Size: 837 935 47 205 390 159 81 93
Cuts at: 3986 4087 5474 1239
Size: 101 1387 2579
Fragments arranged by size:
2579 1387 935 837 390 205 159 101
93 81 47
Bpu10I CC'TnA_GC
Cuts at: 2717 3822 2717
Size: 1105 5709
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3683 3695 4939 5804 6102 3683
Size: 12 1244 865 298 4395
Fragments arranged by size:
4395 1244 865 298 12
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2219 2224 3062 5477 882
Size: 617 720 5 838 2415 2219
Fragments arranged by size:
2415 2219 838 720 617 5
BsaAI yAC'GTr
Cuts at: 493 1204 2298 2410 4605 493
Size: 711 1094 112 2195 2702
Fragments arranged by size:
2702 2195 1094 711 112
BsaBI GATnn'nnATC
Cuts at: 6808 6808
Size: 6814
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4621 5003 5638 275
Size: 53 83 186 936 3088 382 635 1451
Fragments arranged by size:
3088 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5247 5645 5919 6066 6477 5247
Size: 398 274 147 411 5584
Fragments arranged by size:
5584 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6589
Fragments arranged by size:
6589 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 2165 3524 1186
Size: 976 3 1359 4476
Fragments arranged by size:
4476 1359 976 3
BseYI C'CCAG_C
Cuts at: 2035 2880 3387 6017 6513 2035
Size: 845 507 2630 496 2336
Fragments arranged by size:
2630 2336 845 507 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2726 3478 3512 1163
Size: 573 990 752 34 4465
Fragments arranged by size:
4465 990 752 573 34
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3552 3956 4264 5025 6053 1540
Size: 275 186 1551 404 308 761 1028 2301
Fragments arranged by size:
2301 1551 1028 761 404 308 275 186
BsiHKAI G_wGCw'C
Cuts at: 2157 2295 3846 3963 4875 4960 6031 2157
Size: 138 1551 117 912 85 1071 2940
Fragments arranged by size:
2940 1551 1071 912 138 117 85
BsmI GAATG_Cn'
Cuts at: 4325 4418 4325
Size: 93 6721
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2157 2295 2415 2631 2716 2774
Size: 399 240 127 138 120 216 85 58
Cuts at: 2774 2880 2902 2933 3133 3607 3821 3828 3846
Size: 106 22 31 200 474 214 7 18
Cuts at: 3846 3963 4133 4875 4960 6031 1391
Size: 117 170 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 474 399 240 216 214
200 170 138 127 120 117 106 85
85 58 31 22 18 7
BspEI T'CCGG_A
Cuts at: 6477 6477
Size: 6814
BspHI T'CATG_A
Cuts at: 4703 6433 4703
Size: 1730 5084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2748 3447 4099 4169 844
Size: 1904 699 652 70 3489
Fragments arranged by size:
3489 1904 699 652 70
BsrBI CCG'CTC
Cuts at: 1394 1823 3514 4101 4701 1394
Size: 429 1691 587 600 3507
Fragments arranged by size:
3507 1691 600 587 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2840 5309 5483 65
Size: 1183 1592 2469 174 1396
Fragments arranged by size:
2469 1592 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3234 3420 3497 5458 5645 1700
Size: 110 1424 186 77 1961 187 2869
Fragments arranged by size:
2869 1961 1424 187 186 110 77
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6814
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6814
BssSI C'ACGA_G
Cuts at: 2420 4874 5886 2420
Size: 2454 1012 3348
Fragments arranged by size:
3348 2454 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2846 794
Size: 2052 4762
BstBI TT'CG_AA
Cuts at: 5618 5618
Size: 6814
BstEII G'GTnAC_C
Cuts at: 1184 2644 3646 1184
Size: 1460 1002 4352
Fragments arranged by size:
4352 1460 1002
BstXI CCAn_nnnn'nTGG
Cuts at: 2957 3472 3544 4111 2957
Size: 515 72 567 5660
Fragments arranged by size:
5660 567 515 72
BstYI r'GATC_y
Cuts at: 1972 2173 2730 4144 4511 4896 4913 6365 6451
Size: 201 557 1414 367 385 17 1452 86
Cuts at: 6451 6463 6809 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1414 557 385 367 346 201
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2575 2575
Size: 6814
BtgI C'CryG_G
Cuts at: 513 1065 2197 2911 3415 3477 3898 513
Size: 552 1132 714 504 62 421 3429
Fragments arranged by size:
3429 1132 714 552 504 421 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2103 2670 487
Size: 683 56 877 567 4631
Fragments arranged by size:
4631 877 683 567 56
BtsI GCAGTG_nn'
Cuts at: 2504 2631 4065 4330 5124 5144 2504
Size: 127 1434 265 794 20 4174
Fragments arranged by size:
4174 1434 794 265 127 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6814
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6779
DraI TTT'AAA
Cuts at: 4118 4118
Size: 6814
DraIII CAC_nnn'GTG
Cuts at: 3850 3850
Size: 6814
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5821 809
Size: 507 4505 1802
Fragments arranged by size:
4505 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2200 3010 3198
Size: 54 1425 50 275 18 370 810 188
Cuts at: 3198 3236 3418 3474 3549 3953 4261 5150 6779
Size: 38 182 56 75 404 308 889 1629
Cuts at: 6779 8
Size: 43
Fragments arranged by size:
1629 1425 889 810 404 370 308 275
188 182 75 56 54 50 43 38
18
EagI C'GGCC_G
Cuts at: 1537 1812 3549 3953 4261 1537
Size: 275 1737 404 308 4090
Fragments arranged by size:
4090 1737 404 308 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4744 1353
Size: 3391 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5401 5775 5921 387
Size: 1064 138 3812 374 146 1280
Fragments arranged by size:
3812 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2155 3961 2155
Size: 1806 5008
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 3011 3058 3156 3263 3653 3812
Size: 393 444 935 47 98 107 390 159
Cuts at: 3812 3893 3945 3986 4087 4877 5474 6261 6529
Size: 81 52 41 101 790 597 787 268
Cuts at: 6529 1239
Size: 1524
Fragments arranged by size:
1524 935 790 787 597 444 393 390
268 159 107 101 98 81 52 47
41
EcoNI CCTnn'n_nnAGG
Cuts at: 2537 2960 3722 2537
Size: 423 762 5629
Fragments arranged by size:
5629 762 423
EcoO109I rG'GnC_Cy
Cuts at: 2040 2579 2929 2930 3129 3130 3817 3818 4060
Size: 539 350 1 199 1 687 1 242
Cuts at: 4060 2040
Size: 4794
Fragments arranged by size:
4794 687 539 350 242 199 1 1
1
EcoRI G'AATT_C
Cuts at: 4123 4501 4123
Size: 378 6436
FspI TGC'GCA
Cuts at: 3260 5320 3260
Size: 2060 4754
HaeII r_GCGC'y
Cuts at: 1678 3524 4042 5961 1678
Size: 1846 518 1919 2531
Fragments arranged by size:
2531 1919 1846 518
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2729 2761 2990 3022 5455
Size: 32 135 32 1157 32 229 32 2433
Cuts at: 5455 5487 5529 5561 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2433 1157 229 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4158 4404 669
Size: 988 281 2220 246 3079
Fragments arranged by size:
3079 2220 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6814
HpaI GTT'AAC
Cuts at: 4404 4404
Size: 6814
KpnI G_GTAC'C
Cuts at: 2197 4139 2197
Size: 1942 4872
MfeI C'AATT_G
Cuts at: 4413 4413
Size: 6814
MluI A'CGCG_T
Cuts at: 6690 6690
Size: 6814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5928 6112 1305
Size: 4623 184 2007
Fragments arranged by size:
4623 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3012 10
Size: 54 1425 343 1180 3812
Fragments arranged by size:
3812 1425 1180 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3320 3918 4772 5131 5290 518
Size: 648 2154 598 854 359 159 2042
Fragments arranged by size:
2154 2042 854 648 598 359 159
MspA1I CmG'CkG
Cuts at: 1606 2304 2709 2884 3387 3544 3745 4230 4907
Size: 698 405 175 503 157 201 485 677
Cuts at: 4907 6055 6300 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 698 677 503 485 405 245
201 175 157
NaeI GCC'GGC
Cuts at: 3422 3422
Size: 6814
NcoI C'CATG_G
Cuts at: 513 1065 2197 2911 3415 513
Size: 552 1132 714 504 3912
Fragments arranged by size:
3912 1132 714 552 504
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6814
NgoMIV G'CCGG_C
Cuts at: 3420 3420
Size: 6814
NheI G'CTAG_C
Cuts at: 1052 2558 1052
Size: 1506 5308
NotI GC'GGCC_GC
Cuts at: 4261 4261
Size: 6814
NspI r_CATG'y
Cuts at: 1132 1229 3314 3580 4172 5717 1132
Size: 97 2085 266 592 1545 2229
Fragments arranged by size:
2229 2085 1545 592 266 97
PasI CC'CwG_GG
Cuts at: 1348 3225 3828 1348
Size: 1877 603 4334
Fragments arranged by size:
4334 1877 603
PciI A'CATG_T
Cuts at: 3576 5713 3576
Size: 2137 4677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2069 4084 1108
Size: 961 2015 3838
Fragments arranged by size:
3838 2015 961
PfoI T'CCnGG_A
Cuts at: 2109 2259 2890 3963 2109
Size: 150 631 1073 4960
Fragments arranged by size:
4960 1073 631 150
PmeI GTTT'AAAC
Cuts at: 4118 4118
Size: 6814
PmlI CAC'GTG
Cuts at: 2298 2410 2298
Size: 112 6702
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4883 4915 6396 6428 4883
Size: 32 1481 32 5269
Fragments arranged by size:
5269 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2040 4060 2040
Size: 2020 4794
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6814
PsiI TTA'TAA
Cuts at: 4384 4384
Size: 6814
PspOMI G'GGCC_C
Cuts at: 2929 3129 3817 2929
Size: 200 688 5926
Fragments arranged by size:
5926 688 200
PspXI vC'TCGA_Gb
Cuts at: 2516 2516
Size: 6814
PstI C_TGCA'G
Cuts at: 830 2824 3939 4166 830
Size: 1994 1115 227 3478
Fragments arranged by size:
3478 1994 1115 227
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6814
PvuII CAG'CTG
Cuts at: 2709 2884 3544 2709
Size: 175 660 5979
Fragments arranged by size:
5979 660 175
SacI G_AGCT'C
Cuts at: 2157 3963 2157
Size: 1806 5008
SalI G'TCGA_C
Cuts at: 1936 4156 1936
Size: 2220 4594
SanDI GG'GwC_CC
Cuts at: 2040 2040
Size: 6814
SbfI CC_TGCA'GG
Cuts at: 4166 4166
Size: 6814
ScaI AGT'ACT
Cuts at: 5062 6774 5062
Size: 1712 5102
SfcI C'TryA_G
Cuts at: 826 1045 2788 2820 2992 3935 4162 5297 5978
Size: 219 1743 32 172 943 227 1135 681
Cuts at: 5978 6169 826
Size: 191 1471
Fragments arranged by size:
1743 1471 1135 943 681 227 219 191
172 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6814
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6814
SmaI CCC'GGG
Cuts at: 1872 4141 1872
Size: 2269 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2516 3698 3710 4918 5819 6081
Size: 197 926 573 1182 12 1208 901 262
Cuts at: 6081 820
Size: 1553
Fragments arranged by size:
1553 1208 1182 926 901 573 262 197
12
SnaBI TAC'GTA
Cuts at: 493 4605 493
Size: 4112 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6814
SphI G_CATG'C
Cuts at: 1132 4172 1132
Size: 3040 3774
SspI AAT'ATT
Cuts at: 5 52 4738 5
Size: 47 4686 2081
Fragments arranged by size:
4686 2081 47
StuI AGG'CCT
Cuts at: 1517 2318 2966 3906 1517
Size: 801 648 940 4425
Fragments arranged by size:
4425 940 801 648
StyI C'CwwG_G
Cuts at: 513 1065 2094 2197 2229 2583 2911 2956 3318
Size: 552 1029 103 32 354 328 45 362
Cuts at: 3318 3357 3415 4253 513
Size: 39 58 838 3074
Fragments arranged by size:
3074 1029 838 552 362 354 328 103
58 45 39 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2636 2764 3590 3634 4841 4858 5011
Size: 1255 1056 128 826 44 1207 17 153
Cuts at: 5011 5196 5641 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1207 1056 826 445 185 153
128 44 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2103 5060 6772
Size: 274 80 33 51 1443 126 2957 1712
Cuts at: 6772 96
Size: 138
Fragments arranged by size:
2957 1712 1443 274 138 126 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3204 3236 3446 3478 3745 3777 6774 6806 3204
Size: 32 210 32 267 32 2997 32 3212
Fragments arranged by size:
3212 2997 267 210 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2642 4058 1316
Size: 1326 1416 4072
Fragments arranged by size:
4072 1416 1326
XbaI T'CTAG_A
Cuts at: 4150 4150
Size: 6814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2530 2011
Size: 519 6295
XhoI C'TCGA_G
Cuts at: 1943 2516 1943
Size: 573 6241
XmaI C'CCGG_G
Cuts at: 1870 4139 1870
Size: 2269 4545
XmnI GAAnn'nnTTC
Cuts at: 4943 4943
Size: 6814
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4622 276
Size: 53 83 186 936 3088 2468
Fragments arranged by size:
3088 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PsrI RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI