Restriction map for
pFN21AE3504
(Circular) (Six-base) MAPSORT of: pFN21AE3504.seq Check: 1365 from: 1 to: 7294
pFN21AE3504 7294 bp
With 182 enzymes: SgfI *
June 20, 2019 11:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5104 278
Size: 53 83 186 936 3568 2468
Fragments arranged by size:
3568 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2136 2268 4637 1937
Size: 199 132 2369 4594
Fragments arranged by size:
4594 2369 199 132
Acc65I G'GTAC_C
Cuts at: 4615 4615
Size: 7294
AclI AA'CG_TT
Cuts at: 1563 5421 5794 1563
Size: 3858 373 3063
Fragments arranged by size:
3858 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2199 3005 3038 3052 3623 5357 6741 7009
Size: 567 806 33 14 571 1734 1384 268
Cuts at: 7009 1632
Size: 1917
Fragments arranged by size:
1917 1734 1384 806 571 567 268 33
14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7097
AflIII A'CryG_T
Cuts at: 1203 2230 2287 2544 4566 6193 7170 1203
Size: 1027 57 257 2022 1627 977 1327
Fragments arranged by size:
2022 1627 1327 1027 977 257 57
AgeI A'CCGG_T
Cuts at: 6125 6125
Size: 7294
AhdI GACnn_n'nnGTC
Cuts at: 4397 6023 4397
Size: 1626 5668
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3360 1184
Size: 2176 5118
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2779 2811 2779
Size: 32 7262
AlwNI CAG_nnn'CTG
Cuts at: 4142 5037 6609 4142
Size: 895 1572 4827
Fragments arranged by size:
4827 1572 895
ApaLI G'TGCA_C
Cuts at: 5351 6507 5351
Size: 1156 6138
ApoI r'AATT_y
Cuts at: 1493 2341 3080 3320 3353 3797 4603 4829 4981
Size: 848 739 240 33 444 806 226 152
Cuts at: 4981 7090 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 848 806 739 444 240 226
152 33
AseI AT'TA_AT
Cuts at: 160 1032 5848 160
Size: 872 4816 1606
Fragments arranged by size:
4816 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7294
AvaI C'yCGr_G
Cuts at: 1870 1943 4619 1870
Size: 73 2676 4545
Fragments arranged by size:
4545 2676 73
BamHI G'GATC_C
Cuts at: 2845 4624 4991 2845
Size: 1779 367 5148
Fragments arranged by size:
5148 1779 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 4049 4615 6070 618
Size: 325 217 627 231 2031 566 1455 1842
Fragments arranged by size:
2031 1842 1455 627 566 325 231 217
BanII G_rGCy'C
Cuts at: 1391 2919 4613 1391
Size: 1528 1694 4072
Fragments arranged by size:
4072 1694 1528
BbeI G_GCGC'C
Cuts at: 2022 4053 2022
Size: 2031 5263
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3595 4326 928
Size: 971 1696 731 3896
Fragments arranged by size:
3896 1696 971 731
BbvCI CC'TCA_GC
Cuts at: 2939 2939
Size: 7294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2095 2129 5485 5519 1903
Size: 34 50 34 74 34 3356 34 3678
Fragments arranged by size:
3678 3356 74 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4578 5186 6396 4578
Size: 608 1210 5476
Fragments arranged by size:
5476 1210 608
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7294
BfrBI ATG'CAT
Cuts at: 2199 2899 2199
Size: 700 6594
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3521 4058 5905
Size: 107 122 71 631 605 1849 537 1847
Cuts at: 5905 136
Size: 1525
Fragments arranged by size:
1849 1847 1525 631 605 537 122 107
71
BglII A'GATC_T
Cuts at: 2359 7289 2359
Size: 4930 2364
BlpI GC'TnA_GC
Cuts at: 4104 4711 4104
Size: 607 6687
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2118 2978 3688 5355 6511 1790
Size: 304 24 860 710 1667 1156 2573
Fragments arranged by size:
2573 1667 1156 860 710 304 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7294
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2792 3793 5025
Size: 416 53 83 399 24 1375 1001 1232
Cuts at: 5025 5983 442
Size: 958 1753
Fragments arranged by size:
1753 1375 1232 1001 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7294
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2678 2678
Size: 7294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2762 2903 3697 4167 4173 5954 1239
Size: 1523 141 794 470 6 1781 2579
Fragments arranged by size:
2579 1781 1523 794 470 141 6
Bpu10I CC'TnA_GC
Cuts at: 2939 2939
Size: 7294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5419 6284 6582 5419
Size: 865 298 6131
Fragments arranged by size:
6131 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5957 882
Size: 617 4458 2219
Fragments arranged by size:
4458 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2547 3611 5085 493
Size: 711 1343 1064 1474 2702
Fragments arranged by size:
2702 1474 1343 1064 711
BsaBI GATnn'nnATC
Cuts at: 7288 7288
Size: 7294
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 2312 4050 5101
Size: 53 83 186 936 486 293 1738 1051
Cuts at: 5101 5483 6118 275
Size: 382 635 1451
Fragments arranged by size:
1738 1451 1051 936 635 486 382 293
186 83 53
BsaWI w'CCGG_w
Cuts at: 3211 5727 6125 6399 6546 6957 3211
Size: 2516 398 274 147 411 3548
Fragments arranged by size:
3548 2516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2779 2809 4140 4170 1178
Size: 30 165 30 1376 30 1331 30 4302
Fragments arranged by size:
4302 1376 1331 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 3263 3316 1186
Size: 1294 783 53 5164
Fragments arranged by size:
5164 1294 783 53
BseYI C'CCAG_C
Cuts at: 2592 6497 6993 2592
Size: 3905 496 2893
Fragments arranged by size:
3905 2893 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3773 1163
Size: 573 2037 4684
Fragments arranged by size:
4684 2037 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4744 5505 6533 1540
Size: 275 186 2743 761 1028 2301
Fragments arranged by size:
2743 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3255 4285 5355 5440 6511 3255
Size: 1030 1070 85 1071 4038
Fragments arranged by size:
4038 1071 1070 1030 85
BsmI GAATG_Cn'
Cuts at: 3444 4433 4805 4898 3444
Size: 989 372 93 5840
Fragments arranged by size:
5840 989 372 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2118 2919 2978 3255 3688 4285
Size: 399 304 24 801 59 277 433 597
Cuts at: 4285 4613 5355 5440 6511 1391
Size: 328 742 85 1071 2174
Fragments arranged by size:
2174 1071 801 742 597 433 399 328
304 277 85 59 24
BspEI T'CCGG_A
Cuts at: 6957 6957
Size: 7294
BspHI T'CATG_A
Cuts at: 5183 6913 5183
Size: 1730 5564
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2658 4649 844
Size: 1814 1991 3489
Fragments arranged by size:
3489 1991 1814
BsrBI CCG'CTC
Cuts at: 1394 1823 5181 1394
Size: 429 3358 3507
Fragments arranged by size:
3507 3358 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5789 5963 65
Size: 1183 4541 174 1396
Fragments arranged by size:
4541 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5938 6125 1700
Size: 110 4128 187 2869
Fragments arranged by size:
4128 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7294
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7294
BssSI C'ACGA_G
Cuts at: 5354 6366 5354
Size: 1012 6282
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3036 3450 3701 4142 794
Size: 2242 414 251 441 3946
Fragments arranged by size:
3946 2242 441 414 251
BstBI TT'CG_AA
Cuts at: 2248 6098 2248
Size: 3850 3444
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7294
BstXI CCAn_nnnn'nTGG
Cuts at: 2547 2628 2547
Size: 81 7213
BstYI r'GATC_y
Cuts at: 1972 2359 2845 3136 3273 4293 4409 4624 4991
Size: 387 486 291 137 1020 116 215 367
Cuts at: 4991 5376 5393 6845 6931 6943 7289 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1020 486 387 385 367 346
291 215 137 116 86 17 12
BstZ17I GTA'TAC
Cuts at: 2269 2269
Size: 7294
BtgI C'CryG_G
Cuts at: 513 1065 3516 4218 513
Size: 552 2451 702 3589
Fragments arranged by size:
3589 2451 702 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3429 487
Size: 683 56 2203 4352
Fragments arranged by size:
4352 2203 683 56
BtsI GCAGTG_nn'
Cuts at: 3688 4810 5604 5624 3688
Size: 1122 794 20 5358
Fragments arranged by size:
5358 1122 794 20
ClaI AT'CG_AT
Cuts at: 1554 3963 1554
Size: 2409 4885
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4511 4546 529
Size: 35 3947 35 3277
Fragments arranged by size:
3947 3277 35 35
DraI TTT'AAA
Cuts at: 2382 4598 2382
Size: 2216 5078
DraIII CAC_nnn'GTG
Cuts at: 3370 3370
Size: 7294
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2606 2687 6301 809
Size: 507 1290 81 3614 1802
Fragments arranged by size:
3614 1802 1290 507 81
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2856 3513 3987
Size: 54 1425 50 275 18 1026 657 474
Cuts at: 3987 4741 5630 7259 8
Size: 754 889 1629 43
Fragments arranged by size:
1629 1425 1026 889 754 657 474 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4741 1537
Size: 275 2929 4090
Fragments arranged by size:
4090 2929 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4083 5224 1353
Size: 2730 1141 3423
Fragments arranged by size:
3423 2730 1141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5881 6255 6401 387
Size: 1064 138 4292 374 146 1280
Fragments arranged by size:
4292 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2762 2903 3005 3038 3052 3623
Size: 393 567 563 141 102 33 14 571
Cuts at: 3623 3697 4167 4173 5357 5954 6741 7009 1239
Size: 74 470 6 1184 597 787 268 1524
Fragments arranged by size:
1524 1184 787 597 571 567 563 470
393 268 141 102 74 33 14 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2221 3331 4540 2221
Size: 1110 1209 4975
Fragments arranged by size:
4975 1209 1110
EcoO109I rG'GnC_Cy
Cuts at: 2536 4391 4545 2536
Size: 1855 154 5285
Fragments arranged by size:
5285 1855 154
EcoRI G'AATT_C
Cuts at: 2341 3080 3320 3353 4603 4981 2341
Size: 739 240 33 1250 378 4654
Fragments arranged by size:
4654 1250 739 378 240 33
EcoRV GAT'ATC
Cuts at: 3835 3835
Size: 7294
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2435 2725 2435
Size: 290 7004
FspI TGC'GCA
Cuts at: 5800 5800
Size: 7294
HaeII r_GCGC'y
Cuts at: 1678 2014 2022 4053 6441 1678
Size: 336 8 2031 2388 2531
Fragments arranged by size:
2531 2388 2031 336 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3956 3988 4383 4415 5935
Size: 32 135 32 2384 32 395 32 1520
Cuts at: 5935 5967 6009 6041 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2384 1520 395 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4638 4884 669
Size: 988 281 2700 246 3079
Fragments arranged by size:
3079 2700 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7294
HpaI GTT'AAC
Cuts at: 4884 4884
Size: 7294
KasI G'GCGC_C
Cuts at: 2018 4049 2018
Size: 2031 5263
KpnI G_GTAC'C
Cuts at: 4619 4619
Size: 7294
MfeI C'AATT_G
Cuts at: 2487 3941 4893 2487
Size: 1454 952 4888
Fragments arranged by size:
4888 1454 952
MluI A'CGCG_T
Cuts at: 7170 7170
Size: 7294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2609 3164 6408 6592 1305
Size: 1304 555 3244 184 2007
Fragments arranged by size:
3244 2007 1304 555 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2858 3515 10
Size: 54 1425 343 1026 657 3789
Fragments arranged by size:
3789 1425 1026 657 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2898 3161 4217 5252 5611 5770 518
Size: 648 1732 263 1056 1035 359 159 2042
Fragments arranged by size:
2042 1732 1056 1035 648 359 263 159
MspA1I CmG'CkG
Cuts at: 1606 2046 2596 3036 3985 4006 4111 4710 5387
Size: 440 550 440 949 21 105 599 677
Cuts at: 5387 6535 6780 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 949 677 599 550 440 440
245 105 21
NarI GG'CG_CC
Cuts at: 2019 4050 2019
Size: 2031 5263
NcoI C'CATG_G
Cuts at: 513 1065 3516 4218 513
Size: 552 2451 702 3589
Fragments arranged by size:
3589 2451 702 552
NdeI CA'TA_TG
Cuts at: 387 2895 4420 387
Size: 2508 1525 3261
Fragments arranged by size:
3261 2508 1525
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7294
NotI GC'GGCC_GC
Cuts at: 4741 4741
Size: 7294
NsiI A_TGCA'T
Cuts at: 2201 2901 2201
Size: 700 6594
NspI r_CATG'y
Cuts at: 1132 1229 2035 2101 2234 2275 2291 2738 3289
Size: 97 806 66 133 41 16 447 551
Cuts at: 3289 4017 4570 4652 6197 1132
Size: 728 553 82 1545 2229
Fragments arranged by size:
2229 1545 806 728 553 551 447 133
97 82 66 41 16
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7294
PciI A'CATG_T
Cuts at: 2230 2287 4566 6193 2230
Size: 57 2279 1627 3331
Fragments arranged by size:
3331 2279 1627 57
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2996 1108
Size: 1888 5406
PfoI T'CCnGG_A
Cuts at: 2919 2964 2919
Size: 45 7249
PmeI GTTT'AAAC
Cuts at: 4598 4598
Size: 7294
PmlI CAC'GTG
Cuts at: 2547 2547
Size: 7294
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2779 2811 3260 3292 5363 5395 6876 6908 2779
Size: 32 449 32 2071 32 1481 32 3165
Fragments arranged by size:
3165 2071 1481 449 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2536 4391 2536
Size: 1855 5439
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7294
PsiI TTA'TAA
Cuts at: 4864 4864
Size: 7294
PstI C_TGCA'G
Cuts at: 830 2068 4646 830
Size: 1238 2578 3478
Fragments arranged by size:
3478 2578 1238
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7294
PvuII CAG'CTG
Cuts at: 2596 3036 3985 2596
Size: 440 949 5905
Fragments arranged by size:
5905 949 440
SalI G'TCGA_C
Cuts at: 1936 4636 1936
Size: 2700 4594
SapI GCTCTTCn'nnn_
Cuts at: 4083 4083
Size: 7294
SbfI CC_TGCA'GG
Cuts at: 4646 4646
Size: 7294
ScaI AGT'ACT
Cuts at: 5542 7254 5542
Size: 1712 5582
SexAI A'CCwGG_T
Cuts at: 3366 3366
Size: 7294
SfcI C'TryA_G
Cuts at: 826 1045 2064 4642 5777 6458 6649 826
Size: 219 1019 2578 1135 681 191 1471
Fragments arranged by size:
2578 1471 1135 1019 681 219 191
SfoI GGC'GCC
Cuts at: 2020 4051 2020
Size: 2031 5263
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7294
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7294
SmaI CCC'GGG
Cuts at: 1872 4621 1872
Size: 2749 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5398 6299 6561 820
Size: 197 926 3455 901 262 1553
Fragments arranged by size:
3455 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5085 493
Size: 4592 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7294
SphI G_CATG'C
Cuts at: 1132 2035 2738 4652 1132
Size: 903 703 1914 3774
Fragments arranged by size:
3774 1914 903 703
SspI AAT'ATT
Cuts at: 5 52 2461 3117 5218 5
Size: 47 2409 656 2101 2081
Fragments arranged by size:
2409 2101 2081 656 47
StuI AGG'CCT
Cuts at: 1517 3296 3644 1517
Size: 1779 348 5167
Fragments arranged by size:
5167 1779 348
StyI C'CwwG_G
Cuts at: 513 1065 3516 4218 4733 513
Size: 552 2451 702 515 3074
Fragments arranged by size:
3074 2451 702 552 515
SwaI ATTT'AAAT
Cuts at: 2382 2382
Size: 7294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2606 4495 5321 5338 5491 5676 6121
Size: 1255 1026 1889 826 17 153 185 445
Cuts at: 6121 325
Size: 1498
Fragments arranged by size:
1889 1498 1255 1026 826 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4029 4569 5540
Size: 274 80 33 51 1443 2052 540 971
Cuts at: 5540 7252 96
Size: 1712 138
Fragments arranged by size:
2052 1712 1443 971 540 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4491 4523 7254 7286 4491
Size: 32 2731 32 4499
Fragments arranged by size:
4499 2731 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2132 1316
Size: 816 6478
XbaI T'CTAG_A
Cuts at: 2362 4630 2362
Size: 2268 5026
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2600 2600
Size: 7294
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7294
XmaI C'CCGG_G
Cuts at: 1870 4619 1870
Size: 2749 4545
XmnI GAAnn'nnTTC
Cuts at: 3444 3879 5423 3444
Size: 435 1544 5315
Fragments arranged by size:
5315 1544 435
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5102 276
Size: 53 83 186 936 3568 2468
Fragments arranged by size:
3568 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BsiWI BsmBI
Bsu36I EcoICRI FseI FspAI NaeI NgoMIV NruI PacI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SrfI