Restriction map for
pFN21AE3502
(Circular) (Six-base) MAPSORT of: pFN21AE3502.seq Check: 8832 from: 1 to: 9451
pFN21AE3502 9451 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2064 2064
Size: 9451
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7261 278
Size: 53 83 186 936 5725 2468
Fragments arranged by size:
5725 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4656 6794 1937
Size: 2719 2138 4594
Fragments arranged by size:
4594 2719 2138
Acc65I G'GTAC_C
Cuts at: 3550 3580 6772 3550
Size: 30 3192 6229
Fragments arranged by size:
6229 3192 30
AclI AA'CG_TT
Cuts at: 1563 7578 7951 1563
Size: 6015 373 3063
Fragments arranged by size:
6015 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2474 2592 2778 3206 3873 4320 4767 5574
Size: 842 118 186 428 667 447 447 807
Cuts at: 5574 5921 5938 6111 6218 6268 6459 6626 7514
Size: 347 17 173 107 50 191 167 888
Cuts at: 7514 8898 9166 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 888 842 807 667 447 447
428 347 268 191 186 173 167 118
107 50 17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9254
AflIII A'CryG_T
Cuts at: 1203 2965 5610 6102 8350 9327 1203
Size: 1762 2645 492 2248 977 1327
Fragments arranged by size:
2645 2248 1762 1327 977 492
AgeI A'CCGG_T
Cuts at: 6162 8282 6162
Size: 2120 7331
AhdI GACnn_n'nnGTC
Cuts at: 5265 6103 8180 5265
Size: 838 2077 6536
Fragments arranged by size:
6536 2077 838
AleI CACnn'nnGTG
Cuts at: 1166 5955 1166
Size: 4789 4662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9451
AlwNI CAG_nnn'CTG
Cuts at: 3268 3722 5363 5378 6089 6323 7194 8766 3268
Size: 454 1641 15 711 234 871 1572 3953
Fragments arranged by size:
3953 1641 1572 871 711 454 234 15
ApaLI G'TGCA_C
Cuts at: 3276 6640 7508 8664 3276
Size: 3364 868 1156 4063
Fragments arranged by size:
4063 3364 1156 868
ApoI r'AATT_y
Cuts at: 1493 2893 3560 6760 6986 7138 9247 1493
Size: 1400 667 3200 226 152 2109 1697
Fragments arranged by size:
3200 2109 1697 1400 667 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8005 160
Size: 872 6973 1606
Fragments arranged by size:
6973 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9451
AvaI C'yCGr_G
Cuts at: 1870 1943 3333 6645 6776 1870
Size: 73 1390 3312 131 4545
Fragments arranged by size:
4545 3312 1390 131 73
AvrII C'CTAG_G
Cuts at: 3467 3467
Size: 9451
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3810 3843 3810
Size: 33 9418
BamHI G'GATC_C
Cuts at: 2062 6781 7148 2062
Size: 4719 367 4365
Fragments arranged by size:
4719 4365 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2370 2662 3307 3403 3463
Size: 325 217 627 583 292 645 96 60
Cuts at: 3463 3550 3580 3637 3667 3952 4182 4726 4963
Size: 87 30 57 30 285 230 544 237
Cuts at: 4963 6028 6772 8227 618
Size: 1065 744 1455 1842
Fragments arranged by size:
1842 1455 1065 744 645 627 583 544
325 292 285 237 230 217 96 87
60 57 30 30
BanII G_rGCy'C
Cuts at: 1391 2767 5227 6186 6732 6770 1391
Size: 1376 2460 959 546 38 4072
Fragments arranged by size:
4072 2460 1376 959 546 38
BbeI G_GCGC'C
Cuts at: 4186 4967 4186
Size: 781 8670
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2147 5752 5909 928
Size: 971 248 3605 157 4470
Fragments arranged by size:
4470 3605 971 248 157
BbvCI CC'TCA_GC
Cuts at: 4428 4428
Size: 9451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2391 2425 7642 7676 1903
Size: 34 454 34 5217 34 3678
Fragments arranged by size:
5217 3678 454 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3505 3811 7343 8553 3505
Size: 306 3532 1210 4403
Fragments arranged by size:
4403 3532 1210 306
BclI T'GATC_A
Cuts at: 1548 4588 4843 4882 5380 6073 6223 1548
Size: 3040 255 39 498 693 150 4776
Fragments arranged by size:
4776 3040 693 498 255 150 39
BfrBI ATG'CAT
Cuts at: 6023 6069 6023
Size: 46 9405
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3184 8062 136
Size: 107 122 71 631 605 1512 4878 1525
Fragments arranged by size:
4878 1525 1512 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4364 5637 9446 4364
Size: 1273 3809 4369
Fragments arranged by size:
4369 3809 1273
BlpI GC'TnA_GC
Cuts at: 2497 6289 6868 2497
Size: 3792 579 5080
Fragments arranged by size:
5080 3792 579
Bme1580I G_kGCm'C
Cuts at: 1790 2605 3280 3466 3955 6031 6644 7512 8668
Size: 815 675 186 489 2076 613 868 1156
Cuts at: 8668 1790
Size: 2573
Fragments arranged by size:
2573 2076 1156 868 815 675 613 489
186
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9451
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 7182 8140 442
Size: 416 53 83 399 24 5765 958 1753
Fragments arranged by size:
5765 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2044 3674 8111 1239
Size: 805 1630 4437 2579
Fragments arranged by size:
4437 2579 1630 805
Bpu10I CC'TnA_GC
Cuts at: 3282 3303 3357 4428 5541 3282
Size: 21 54 1071 1113 7192
Fragments arranged by size:
7192 1113 1071 54 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6188 7576 8441 8739 6188
Size: 1388 865 298 6900
Fragments arranged by size:
6900 1388 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4129 4440 4563 5275 6166 8114 882
Size: 617 2630 311 123 712 891 1948 2219
Fragments arranged by size:
2630 2219 1948 891 712 617 311 123
BsaAI yAC'GTr
Cuts at: 493 1204 7242 493
Size: 711 6038 2702
Fragments arranged by size:
6038 2702 711
BsaBI GATnn'nnATC
Cuts at: 5795 6300 9445 5795
Size: 505 3145 5801
Fragments arranged by size:
5801 3145 505
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4183 4964 7258 7640
Size: 53 83 186 936 2650 781 2294 382
Cuts at: 7640 8275 275
Size: 635 1451
Fragments arranged by size:
2650 2294 1451 936 781 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4704 6162 7884 8282 8556 8703 9114 4704
Size: 1458 1722 398 274 147 411 5041
Fragments arranged by size:
5041 1722 1458 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2239 2269 1178
Size: 30 165 30 836 30 8360
Fragments arranged by size:
8360 836 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2760 5330 1186
Size: 1574 2570 5307
Fragments arranged by size:
5307 2570 1574
BseYI C'CCAG_C
Cuts at: 8654 9150 8654
Size: 496 8955
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2421 3693 5118 5349 1163
Size: 573 685 1272 1425 231 5265
Fragments arranged by size:
5265 1425 1272 685 573 231
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6901 7662 8690 1540
Size: 275 186 4900 761 1028 2301
Fragments arranged by size:
4900 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3280 3438 3831 6186 6644 6732 7512 7597 8668
Size: 158 393 2355 458 88 780 85 1071
Cuts at: 8668 3280
Size: 4063
Fragments arranged by size:
4063 2355 1071 780 458 393 158 88
85
BsmI GAATG_Cn'
Cuts at: 6962 7055 6962
Size: 93 9358
BsmBI CGTCTCn'nnnn_
Cuts at: 5295 5295
Size: 9451
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2605 2767 3280 3438 3466 3831 3955
Size: 399 815 162 513 158 28 365 124
Cuts at: 3955 5227 6031 6186 6644 6732 6770 7512 7597
Size: 1272 804 155 458 88 38 742 85
Cuts at: 7597 8668 1391
Size: 1071 2174
Fragments arranged by size:
2174 1272 1071 815 804 742 513 458
399 365 162 158 155 124 88 85
38 28
BspEI T'CCGG_A
Cuts at: 4704 9114 4704
Size: 4410 5041
BspHI T'CATG_A
Cuts at: 2134 5383 6705 7340 9070 2134
Size: 3249 1322 635 1730 2515
Fragments arranged by size:
3249 2515 1730 1322 635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2064 2396 3469 5293 6603 6806 844
Size: 1220 332 1073 1824 1310 203 3489
Fragments arranged by size:
3489 1824 1310 1220 1073 332 203
BsrBI CCG'CTC
Cuts at: 1394 1823 7338 1394
Size: 429 5515 3507
Fragments arranged by size:
5515 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4864 7946 8120 65
Size: 1183 3616 3082 174 1396
Fragments arranged by size:
3616 3082 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6162 8095 8282 1700
Size: 110 4352 1933 187 2869
Fragments arranged by size:
4352 2869 1933 187 110
BsrGI T'GTAC_A
Cuts at: 96 6527 96
Size: 6431 3020
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9451
BssSI C'ACGA_G
Cuts at: 3107 7511 8523 3107
Size: 4404 1012 4035
Fragments arranged by size:
4404 4035 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3138 794
Size: 2344 7107
BstBI TT'CG_AA
Cuts at: 5203 8255 5203
Size: 3052 6399
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9451
BstXI CCAn_nnnn'nTGG
Cuts at: 2752 6592 2752
Size: 3840 5611
BstYI r'GATC_y
Cuts at: 1972 2044 2062 2269 4364 5637 6480 6781 7148
Size: 72 18 207 2095 1273 843 301 367
Cuts at: 7148 7533 7550 9002 9088 9100 9446 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2095 1977 1452 1273 843 385 367 346
301 207 86 72 18 17 12
BstZ17I GTA'TAC
Cuts at: 4657 4657
Size: 9451
Bsu36I CC'TnA_GG
Cuts at: 2667 3864 5529 2667
Size: 1197 1665 6589
Fragments arranged by size:
6589 1665 1197
BtgI C'CryG_G
Cuts at: 513 1065 2004 5086 513
Size: 552 939 3082 4878
Fragments arranged by size:
4878 3082 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8712
Fragments arranged by size:
8712 683 56
BtsI GCAGTG_nn'
Cuts at: 3257 5164 5179 6051 6967 7761 7781 3257
Size: 1907 15 872 916 794 20 4927
Fragments arranged by size:
4927 1907 916 872 794 20 15
ClaI AT'CG_AT
Cuts at: 1554 6312 1554
Size: 4758 4693
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3522 3557 3795 3830 5766 5801 529
Size: 35 2958 35 238 35 1936 35 4179
Fragments arranged by size:
4179 2958 1936 238 35 35 35 35
DraI TTT'AAA
Cuts at: 2451 4630 6755 2451
Size: 2179 2125 5147
Fragments arranged by size:
5147 2179 2125
DraIII CAC_nnn'GTG
Cuts at: 3796 3796
Size: 9451
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2036 8458 809
Size: 507 720 6422 1802
Fragments arranged by size:
6422 1802 720 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6898 7787 9416
Size: 54 1425 50 275 18 5068 889 1629
Cuts at: 9416 8
Size: 43
Fragments arranged by size:
5068 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6898 1537
Size: 275 5086 4090
Fragments arranged by size:
5086 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2259 2807 3195 4420 4743 5161 5208 5273
Size: 906 548 388 1225 323 418 47 65
Cuts at: 5273 6476 6720 7381 1353
Size: 1203 244 661 3423
Fragments arranged by size:
3423 1225 1203 906 661 548 418 388
323 244 65 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5744 8038 8412 8558 387
Size: 1064 138 4155 2294 374 146 1280
Fragments arranged by size:
4155 2294 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 6184 6730 6184
Size: 546 8905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2044 2474 2592 2778 3206 3674 3873
Size: 393 412 430 118 186 428 468 199
Cuts at: 3873 4320 4767 5574 5921 5938 6111 6218 6268
Size: 447 447 807 347 17 173 107 50
Cuts at: 6268 6459 6626 7514 8111 8898 9166 1239
Size: 191 167 888 597 787 268 1524
Fragments arranged by size:
1524 888 807 787 597 468 447 447
430 428 412 393 347 268 199 191
186 173 167 118 107 50 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2199 5457 5842 2199
Size: 3258 385 5808
Fragments arranged by size:
5808 3258 385
EcoO109I rG'GnC_Cy
Cuts at: 2012 2689 3092 3607 2012
Size: 677 403 515 7856
Fragments arranged by size:
7856 677 515 403
EcoRI G'AATT_C
Cuts at: 6760 7138 6760
Size: 378 9073
EcoRV GAT'ATC
Cuts at: 4204 6302 4204
Size: 2098 7353
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6298 6679 6298
Size: 381 9070
FspI TGC'GCA
Cuts at: 6680 7957 6680
Size: 1277 8174
HaeII r_GCGC'y
Cuts at: 1678 4186 4967 8598 1678
Size: 2508 781 3631 2531
Fragments arranged by size:
3631 2531 2508 781
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2161 2193 2425 2457 2711
Size: 32 135 32 589 32 232 32 254
Cuts at: 2711 2743 3917 3949 3991 4023 4043 4075 4247
Size: 32 1174 32 42 32 20 32 172
Cuts at: 4247 4279 4799 4831 5251 5283 6089 6121 6291
Size: 32 520 32 420 32 806 32 170
Cuts at: 6291 6305 6317 6323 6337 6349 6491 6523 8092
Size: 14 12 6 14 12 142 32 1569
Cuts at: 8092 8124 8166 8198 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1569 1174 806 589 520 420 254
232 172 170 142 135 42 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 20 14
14 12 12 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4606 4719 5566 5937 6244 6795
Size: 988 281 2668 113 847 371 307 551
Cuts at: 6795 7041 669
Size: 246 3079
Fragments arranged by size:
3079 2668 988 847 551 371 307 281
246 113
HindIII A'AGCT_T
Cuts at: 748 4145 748
Size: 3397 6054
HpaI GTT'AAC
Cuts at: 4719 5566 5937 7041 4719
Size: 847 371 1104 7129
Fragments arranged by size:
7129 1104 847 371
KasI G'GCGC_C
Cuts at: 4182 4963 4182
Size: 781 8670
KpnI G_GTAC'C
Cuts at: 3554 3584 6776 3554
Size: 30 3192 6229
Fragments arranged by size:
6229 3192 30
MfeI C'AATT_G
Cuts at: 3900 5991 7050 3900
Size: 2091 1059 6301
Fragments arranged by size:
6301 2091 1059
MluI A'CGCG_T
Cuts at: 9327 9327
Size: 9451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2090 5989 6239 8565 8749 1305
Size: 785 3899 250 2326 184 2007
Fragments arranged by size:
3899 2326 2007 785 250 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7629
Fragments arranged by size:
7629 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2750 4851 4914 5388 5613 5955 6018
Size: 648 1584 2101 63 474 225 342 63
Cuts at: 6018 6566 7409 7768 7927 518
Size: 548 843 359 159 2042
Fragments arranged by size:
2101 2042 1584 843 648 548 474 359
342 225 159 63 63
MspA1I CmG'CkG
Cuts at: 1606 2159 2368 2844 4432 6149 6867 7544 8692
Size: 553 209 476 1588 1717 718 677 1148
Cuts at: 8692 8937 1606
Size: 245 2120
Fragments arranged by size:
2120 1717 1588 1148 718 677 553 476
245 209
NarI GG'CG_CC
Cuts at: 4183 4964 4183
Size: 781 8670
NcoI C'CATG_G
Cuts at: 513 1065 2004 5086 513
Size: 552 939 3082 4878
Fragments arranged by size:
4878 3082 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9451
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9451
NotI GC'GGCC_GC
Cuts at: 6898 6898
Size: 9451
NsiI A_TGCA'T
Cuts at: 6025 6071 6025
Size: 46 9405
NspI r_CATG'y
Cuts at: 1132 1229 2926 2969 5144 5592 5614 6106 6809
Size: 97 1697 43 2175 448 22 492 703
Cuts at: 6809 8354 1132
Size: 1545 2229
Fragments arranged by size:
2229 2175 1697 1545 703 492 448 97
43 22
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9451
PciI A'CATG_T
Cuts at: 2965 5610 6102 8350 2965
Size: 2645 492 2248 4066
Fragments arranged by size:
4066 2645 2248 492
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2847 5327 6546 1108
Size: 1739 2480 1219 4013
Fragments arranged by size:
4013 2480 1739 1219
PfoI T'CCnGG_A
Cuts at: 5269 5269
Size: 9451
PmeI GTTT'AAAC
Cuts at: 6755 6755
Size: 9451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7520 7552 9033 9065 7520
Size: 32 1481 32 7906
Fragments arranged by size:
7906 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2012 2689 3092 2012
Size: 677 403 8371
Fragments arranged by size:
8371 677 403
PshAI GACnn'nnGTC
Cuts at: 1613 5287 1613
Size: 3674 5777
PsiI TTA'TAA
Cuts at: 5738 7021 5738
Size: 1283 8168
PstI C_TGCA'G
Cuts at: 830 3264 3480 6803 830
Size: 2434 216 3323 3478
Fragments arranged by size:
3478 3323 2434 216
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9451
PvuII CAG'CTG
Cuts at: 2159 6149 2159
Size: 3990 5461
SacI G_AGCT'C
Cuts at: 6186 6732 6186
Size: 546 8905
SalI G'TCGA_C
Cuts at: 1936 6793 1936
Size: 4857 4594
SapI GCTCTTCn'nnn_
Cuts at: 3195 6720 3195
Size: 3525 5926
SbfI CC_TGCA'GG
Cuts at: 6803 6803
Size: 9451
ScaI AGT'ACT
Cuts at: 2303 7699 9411 2303
Size: 5396 1712 2343
Fragments arranged by size:
5396 2343 1712
SexAI A'CCwGG_T
Cuts at: 3546 3633 4875 5837 3546
Size: 87 1242 962 7160
Fragments arranged by size:
7160 1242 962 87
SfcI C'TryA_G
Cuts at: 826 1045 3230 3260 3350 3446 3476 5050 5309
Size: 219 2185 30 90 96 30 1574 259
Cuts at: 5309 6513 6799 7934 8615 8806 826
Size: 1204 286 1135 681 191 1471
Fragments arranged by size:
2185 1574 1471 1204 1135 681 286 259
219 191 96 90 30 30
SfoI GGC'GCC
Cuts at: 4184 4965 4184
Size: 781 8670
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9451
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9451
SmaI CCC'GGG
Cuts at: 1872 6647 6778 1872
Size: 4775 131 4545
Fragments arranged by size:
4775 4545 131
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6203 7555 8456 8718 820
Size: 197 926 4260 1352 901 262 1553
Fragments arranged by size:
4260 1553 1352 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 7242 493
Size: 6749 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9451
SphI G_CATG'C
Cuts at: 1132 6809 1132
Size: 5677 3774
SspI AAT'ATT
Cuts at: 5 52 2076 7375 5
Size: 47 2024 5299 2081
Fragments arranged by size:
5299 2081 2024 47
StuI AGG'CCT
Cuts at: 1517 5339 1517
Size: 3822 5629
StyI C'CwwG_G
Cuts at: 513 1065 2004 2101 2790 3179 3467 4976 5086
Size: 552 939 97 689 389 288 1509 110
Cuts at: 5086 5227 6890 513
Size: 141 1663 3074
Fragments arranged by size:
3074 1663 1509 939 689 552 389 288
141 110 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2232 2401 2949 7478 7495 7648 7833
Size: 1255 652 169 548 4529 17 153 185
Cuts at: 7833 8278 325
Size: 445 1498
Fragments arranged by size:
4529 1498 1255 652 548 445 185 169
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2301 3069 6527
Size: 274 80 33 51 1443 324 768 3458
Cuts at: 6527 7697 9409 96
Size: 1170 1712 138
Fragments arranged by size:
3458 1712 1443 1170 768 324 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2196 2228 2239 2271 3138 3170 9411 9443 2196
Size: 32 11 32 867 32 6241 32 2204
Fragments arranged by size:
6241 2204 867 32 32 32 32 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9451
XbaI T'CTAG_A
Cuts at: 6787 6787
Size: 9451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4619 4619
Size: 9451
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9451
XmaI C'CCGG_G
Cuts at: 1870 6645 6776 1870
Size: 4775 131 4545
Fragments arranged by size:
4775 4545 131
XmnI GAAnn'nnTTC
Cuts at: 2487 2636 4281 7580 2487
Size: 149 1645 3299 4358
Fragments arranged by size:
4358 3299 1645 149
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7259 276
Size: 53 83 186 936 5725 2468
Fragments arranged by size:
5725 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BplI BsiWI FseI FspAI
NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SfiI SrfI SwaI