Restriction map for
pFN21AE3496
(Circular) (Six-base) MAPSORT of: pFN21AE3496.seq Check: 6718 from: 1 to: 10615
pFN21AE3496 10615 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3330 3525 5145 6805 3330
Size: 195 1620 1660 7140
Fragments arranged by size:
7140 1660 1620 195
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3663 8425 278
Size: 53 83 186 936 2127 4762 2468
Fragments arranged by size:
4762 2468 2127 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2436 3443 3707 5095 5589 7562 7707 7958
Size: 499 1007 264 1388 494 1973 145 251
Cuts at: 7958 1937
Size: 4594
Fragments arranged by size:
4594 1973 1388 1007 499 494 264 251
145
Acc65I G'GTAC_C
Cuts at: 2562 7936 2562
Size: 5374 5241
AclI AA'CG_TT
Cuts at: 1563 5498 7914 8742 9115 1563
Size: 3935 2416 828 373 3063
Fragments arranged by size:
3935 3063 2416 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2117 3041 6658 8678 10062 10330 1632
Size: 485 924 3617 2020 1384 268 1917
Fragments arranged by size:
3617 2020 1917 1384 924 485 268
AflII C'TTAA_G
Cuts at: 820 1017 4730 5375 820
Size: 197 3713 645 6060
Fragments arranged by size:
6060 3713 645 197
AflIII A'CryG_T
Cuts at: 1203 2222 2431 3351 9514 10491 1203
Size: 1019 209 920 6163 977 1327
Fragments arranged by size:
6163 1327 1019 977 920 209
AgeI A'CCGG_T
Cuts at: 9446 9446
Size: 10615
AhdI GACnn_n'nnGTC
Cuts at: 2244 6257 9344 2244
Size: 4013 3087 3515
Fragments arranged by size:
4013 3515 3087
AleI CACnn'nnGTG
Cuts at: 1166 2013 2954 3354 3635 3884 5558 1166
Size: 847 941 400 281 249 1674 6223
Fragments arranged by size:
6223 1674 941 847 400 281 249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2826 3305 6457 1184
Size: 1642 479 3152 5342
Fragments arranged by size:
5342 3152 1642 479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2477 2509 2566 2598 2477
Size: 32 57 32 10494
Fragments arranged by size:
10494 57 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3529 3556 4720 8358 9930 3529
Size: 27 1164 3638 1572 4214
Fragments arranged by size:
4214 3638 1572 1164 27
ApaI G_GGCC'C
Cuts at: 4792 4792
Size: 10615
ApaLI G'TGCA_C
Cuts at: 8672 9828 8672
Size: 1156 9459
ApoI r'AATT_y
Cuts at: 1493 3644 4868 5984 7924 8150 8302 10411 1493
Size: 2151 1224 1116 1940 226 152 2109 1697
Fragments arranged by size:
2151 2109 1940 1697 1224 1116 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9169 160
Size: 872 8137 1606
Fragments arranged by size:
8137 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10615
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 2182 4248 5252 5448 6718 7940
Size: 73 83 156 2066 1004 196 1270 1222
Cuts at: 7940 1870
Size: 4545
Fragments arranged by size:
4545 2066 1270 1222 1004 196 156 83
73
BamHI G'GATC_C
Cuts at: 2574 6006 7945 8312 2574
Size: 3432 1939 367 4877
Fragments arranged by size:
4877 3432 1939 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2562 3248 6590 6764 7936
Size: 325 217 627 775 686 3342 174 1172
Cuts at: 7936 9391 618
Size: 1455 1842
Fragments arranged by size:
3342 1842 1455 1172 775 686 627 325
217 174
BanII G_rGCy'C
Cuts at: 1391 2724 2810 4185 4545 4792 5272 5385 6716
Size: 1333 86 1375 360 247 480 113 1331
Cuts at: 6716 6810 7934 1391
Size: 94 1124 4072
Fragments arranged by size:
4072 1375 1333 1331 1124 480 360 247
113 94 86
BbeI G_GCGC'C
Cuts at: 3252 6594 3252
Size: 3342 7273
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2315 3655 3849 4689 5419 5845 928
Size: 971 416 1340 194 840 730 426 5698
Fragments arranged by size:
5698 1340 971 840 730 426 416 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 8806 8840 1903
Size: 34 50 34 6785 34 3678
Fragments arranged by size:
6785 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3821 4571 8507 9717 3821
Size: 750 3936 1210 4719
Fragments arranged by size:
4719 3936 1210 750
BclI T'GATC_A
Cuts at: 1548 3909 4596 1548
Size: 2361 687 7567
Fragments arranged by size:
7567 2361 687
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2259 2550 4695
Size: 107 122 71 631 605 587 291 2145
Cuts at: 4695 9226 136
Size: 4531 1525
Fragments arranged by size:
4531 2145 1525 631 605 587 291 122
107 71
BglII A'GATC_T
Cuts at: 2673 6096 10610 2673
Size: 3423 4514 2678
Fragments arranged by size:
4514 3423 2678
BlpI GC'TnA_GC
Cuts at: 4832 8032 4832
Size: 3200 7415
Bme1580I G_kGCm'C
Cuts at: 1790 2772 3147 3944 4792 5370 8676 9832 1790
Size: 982 375 797 848 578 3306 1156 2573
Fragments arranged by size:
3306 2573 1156 982 848 797 578 375
BmgBI CAC'GTC
Cuts at: 1318 3711 3945 4828 6058 6078 6157 6316 1318
Size: 2393 234 883 1230 20 79 159 5617
Fragments arranged by size:
5617 2393 1230 883 234 159 79 20
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3303 6519 6597
Size: 416 53 83 399 24 1886 3216 78
Cuts at: 6597 6642 8346 9304 442
Size: 45 1704 958 1753
Fragments arranged by size:
3216 1886 1753 1704 958 416 399 83
78 53 45 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10615
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6829 6829
Size: 10615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2918 3551 3689 4199 4941 5507 6032 6158
Size: 1679 633 138 510 742 566 525 126
Cuts at: 6158 7686 9275 1239
Size: 1528 1589 2579
Fragments arranged by size:
2579 1679 1589 1528 742 633 566 525
510 138 126
Bpu10I CC'TnA_GC
Cuts at: 4901 4901
Size: 10615
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2996 6721 8740 9605 9903 2996
Size: 3725 2019 865 298 3708
Fragments arranged by size:
3725 3708 2019 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6370 6705 9278 882
Size: 617 4871 335 2573 2219
Fragments arranged by size:
4871 2573 2219 617 335
BsaAI yAC'GTr
Cuts at: 493 1204 2293 8406 493
Size: 711 1089 6113 2702
Fragments arranged by size:
6113 2702 1089 711
BsaBI GATnn'nnATC
Cuts at: 4343 5966 6242 10609 4343
Size: 1623 276 4367 4349
Fragments arranged by size:
4367 4349 1623 276
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3004 3249 3660 6591
Size: 53 83 186 936 1471 245 411 2931
Cuts at: 6591 8422 8804 9439 275
Size: 1831 382 635 1451
Fragments arranged by size:
2931 1831 1471 1451 936 635 411 382
245 186 83 53
BsaWI w'CCGG_w
Cuts at: 2086 4319 4416 5673 9048 9446 9720 9867 10278
Size: 2233 97 1257 3375 398 274 147 411
Cuts at: 10278 2086
Size: 2423
Fragments arranged by size:
3375 2423 2233 1257 411 398 274 147
97
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5207 5237 6146 6176 7810
Size: 30 165 30 3804 30 909 30 1634
Cuts at: 7810 7840 1178
Size: 30 3953
Fragments arranged by size:
3953 3804 1634 909 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2492 2711 2921 4034 4255 5186 5831 7922
Size: 1306 219 210 1113 221 931 645 2091
Cuts at: 7922 1186
Size: 3879
Fragments arranged by size:
3879 2091 1306 1113 931 645 221 219
210
BseYI C'CCAG_C
Cuts at: 2710 5359 6109 6382 6776 6926 6947 6968 6989
Size: 2649 750 273 394 150 21 21 21
Cuts at: 6989 7010 7031 7094 7115 7136 7157 7199 7283
Size: 21 21 63 21 21 21 42 84
Cuts at: 7283 7304 7388 7472 7484 7493 7547 9818 10314
Size: 21 84 84 12 9 54 2271 496
Cuts at: 10314 2710
Size: 3011
Fragments arranged by size:
3011 2649 2271 750 496 394 273 150
84 84 84 63 54 42 21 21
21 21 21 21 21 21 21 12
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2247 3380 3638 4685 5000 1163
Size: 573 511 1133 258 1047 315 6778
Fragments arranged by size:
6778 1133 1047 573 511 315 258
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6402 8065 8826 9854 1540
Size: 275 186 4401 1663 761 1028 2301
Fragments arranged by size:
4401 2301 1663 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4902 5385 5555 5775 8676 8761 9832 4902
Size: 483 170 220 2901 85 1071 5685
Fragments arranged by size:
5685 2901 1071 483 220 170 85
BsiWI C'GTAC_G
Cuts at: 6067 6067
Size: 10615
BsmI GAATG_Cn'
Cuts at: 3429 4233 4975 8126 8219 3429
Size: 804 742 3151 93 5825
Fragments arranged by size:
5825 3151 804 742 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2132 3527 3651 6008 7095 2132
Size: 1395 124 2357 1087 5652
Fragments arranged by size:
5652 2357 1395 1087 124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2724 2772 2810 3147 3944 4185 4545
Size: 399 934 48 38 337 797 241 360
Cuts at: 4545 4792 4902 5272 5370 5385 5555 5775 6716
Size: 247 110 370 98 15 170 220 941
Cuts at: 6716 6810 7934 8676 8761 9832 1391
Size: 94 1124 742 85 1071 2174
Fragments arranged by size:
2174 1124 1071 941 934 797 742 399
370 360 337 247 241 220 170 110
98 94 85 48 38 15
BspEI T'CCGG_A
Cuts at: 2086 4319 10278 2086
Size: 2233 5959 2423
Fragments arranged by size:
5959 2423 2233
BspHI T'CATG_A
Cuts at: 3988 5787 6285 8504 10234 3988
Size: 1799 498 2219 1730 4369
Fragments arranged by size:
4369 2219 1799 1730 498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3330 3525 4959 5022 5145 5305 6805 7970
Size: 2486 195 1434 63 123 160 1500 1165
Cuts at: 7970 844
Size: 3489
Fragments arranged by size:
3489 2486 1500 1434 1165 195 160 123
63
BsrBI CCG'CTC
Cuts at: 1394 1823 2203 2776 3150 3673 4864 4876 5447
Size: 429 380 573 374 523 1191 12 571
Cuts at: 5447 6142 6813 8502 1394
Size: 695 671 1689 3507
Fragments arranged by size:
3507 1689 1191 695 671 573 571 523
429 380 374 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 2887 2910 3153 4080 4356 5241 9110
Size: 1183 1639 23 243 927 276 885 3869
Cuts at: 9110 9284 65
Size: 174 1396
Fragments arranged by size:
3869 1639 1396 1183 927 885 276 243
174 23
BsrFI r'CCGG_y
Cuts at: 1700 1810 3329 3381 3733 4490 6401 6500 6635
Size: 110 1519 52 352 757 1911 99 135
Cuts at: 6635 9259 9446 1700
Size: 2624 187 2869
Fragments arranged by size:
2869 2624 1911 1519 757 352 187 135
110 99 52
BsrGI T'GTAC_A
Cuts at: 96 5925 96
Size: 5829 4786
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10615
BssSI C'ACGA_G
Cuts at: 8675 9687 8675
Size: 1012 9603
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6411 6581 794
Size: 5617 170 4828
Fragments arranged by size:
5617 4828 170
BstBI TT'CG_AA
Cuts at: 9419 9419
Size: 10615
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10615
BstXI CCAn_nnnn'nTGG
Cuts at: 2956 3889 5029 5333 6532 2956
Size: 933 1140 304 1199 7039
Fragments arranged by size:
7039 1199 1140 933 304
BstYI r'GATC_y
Cuts at: 1972 2524 2574 2643 2673 3546 6006 6096 6459
Size: 552 50 69 30 873 2460 90 363
Cuts at: 6459 7945 8312 8697 8714 10166 10252 10264 10610
Size: 1486 367 385 17 1452 86 12 346
Cuts at: 10610 1972
Size: 1977
Fragments arranged by size:
2460 1977 1486 1452 873 552 385 367
363 346 90 86 69 50 30 17
12
BstZ17I GTA'TAC
Cuts at: 7563 7563
Size: 10615
Bsu36I CC'TnA_GG
Cuts at: 2511 2511
Size: 10615
BtgI C'CryG_G
Cuts at: 513 1065 2545 2952 3437 3561 4462 4524 4605
Size: 552 1480 407 485 124 901 62 81
Cuts at: 4605 5685 6531 513
Size: 1080 846 4597
Fragments arranged by size:
4597 1480 1080 901 846 552 485 407
124 81 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5811 487
Size: 683 56 4585 5291
Fragments arranged by size:
5291 4585 683 56
BtsI GCAGTG_nn'
Cuts at: 3642 8131 8925 8945 3642
Size: 4489 794 20 5312
Fragments arranged by size:
5312 4489 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10615
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10580
DraI TTT'AAA
Cuts at: 7919 7919
Size: 10615
DraIII CAC_nnn'GTG
Cuts at: 5151 6341 6761 5151
Size: 1190 420 9005
Fragments arranged by size:
9005 1190 420
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4556 4931 9622 809
Size: 507 3240 375 4691 1802
Fragments arranged by size:
4691 3240 1802 507 375
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2209 2251 2260
Size: 54 1425 50 275 18 379 42 9
Cuts at: 2260 2274 2735 2946 3558 3735 6229 6240 6399
Size: 14 461 211 612 177 2494 11 159
Cuts at: 6399 8062 8951 10580 8
Size: 1663 889 1629 43
Fragments arranged by size:
2494 1663 1629 1425 889 612 461 379
275 211 177 159 54 50 43 42
18 14 11 9
EagI C'GGCC_G
Cuts at: 1537 1812 6399 8062 1537
Size: 275 4587 1663 4090
Fragments arranged by size:
4587 4090 1663 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4010 5129 5817 8545 1353
Size: 2657 1119 688 2728 3423
Fragments arranged by size:
3423 2728 2657 1119 688
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9202 9576 9722 387
Size: 1064 138 7613 374 146 1280
Fragments arranged by size:
7613 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5383 5383
Size: 10615
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2918 3041 3551 3689 4199 4941
Size: 393 485 801 123 510 138 510 742
Cuts at: 4941 5507 6032 6158 6658 7686 8678 9275 10062
Size: 566 525 126 500 1028 992 597 787
Cuts at: 10062 10330 1239
Size: 268 1524
Fragments arranged by size:
1524 1028 992 801 787 742 597 566
525 510 510 500 485 393 268 138
126 123
EcoNI CCTnn'n_nnAGG
Cuts at: 2447 4049 4820 6756 2447
Size: 1602 771 1936 6306
Fragments arranged by size:
6306 1936 1602 771
EcoO109I rG'GnC_Cy
Cuts at: 3724 4788 4789 4813 5992 6133 6723 6731 3724
Size: 1064 1 24 1179 141 590 8 7608
Fragments arranged by size:
7608 1179 1064 590 141 24 8 1
EcoRI G'AATT_C
Cuts at: 5984 7924 8302 5984
Size: 1940 378 8297
Fragments arranged by size:
8297 1940 378
EcoRV GAT'ATC
Cuts at: 2389 2389
Size: 10615
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3793 4783 6313 3793
Size: 990 1530 8095
Fragments arranged by size:
8095 1530 990
FspI TGC'GCA
Cuts at: 2051 3224 3641 5247 5280 9121 2051
Size: 1173 417 1606 33 3841 3545
Fragments arranged by size:
3841 3545 1606 1173 417 33
HaeII r_GCGC'y
Cuts at: 1678 3252 6594 9762 1678
Size: 1574 3342 3168 2531
Fragments arranged by size:
3342 3168 2531 1574
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2230 2262 2851 2883 3403
Size: 32 135 32 658 32 589 32 520
Cuts at: 3403 3435 3531 3563 4411 4443 5207 5239 6243
Size: 32 96 32 848 32 764 32 1004
Cuts at: 6243 6275 9256 9288 9330 9362 1373
Size: 32 2981 32 42 32 2626
Fragments arranged by size:
2981 2626 1004 848 764 658 589 520
135 96 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3708 5096 6262 7959 8205 669
Size: 988 281 1770 1388 1166 1697 246 3079
Fragments arranged by size:
3079 1770 1697 1388 1166 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2162 748
Size: 1414 9201
HpaI GTT'AAC
Cuts at: 8205 8205
Size: 10615
KasI G'GCGC_C
Cuts at: 3248 6590 3248
Size: 3342 7273
KpnI G_GTAC'C
Cuts at: 2566 7940 2566
Size: 5374 5241
MfeI C'AATT_G
Cuts at: 3805 8214 3805
Size: 4409 6206
MluI A'CGCG_T
Cuts at: 10491 10491
Size: 10615
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4038 4348 4764 4839 5034 5898 6920 7277
Size: 2733 310 416 75 195 864 1022 357
Cuts at: 7277 7403 7475 9729 9913 1305
Size: 126 72 2254 184 2007
Fragments arranged by size:
2733 2254 2007 1022 864 416 357 310
195 184 126 75 72
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2253 2262 2276 2948 3560
Size: 54 1425 343 421 9 14 672 612
Cuts at: 3560 6231 6242 10
Size: 2671 11 4383
Fragments arranged by size:
4383 2671 1425 672 612 421 343 54
14 11 9
MslI CAynn'nnrTG
Cuts at: 518 1166 2013 2619 2954 3354 3635 3834 3884
Size: 648 847 606 335 400 281 199 50
Cuts at: 3884 4170 5027 5306 5558 6503 8573 8932 9091
Size: 286 857 279 252 945 2070 359 159
Cuts at: 9091 518
Size: 2042
Fragments arranged by size:
2070 2042 945 857 847 648 606 400
359 335 286 281 279 252 199 159
50
MspA1I CmG'CkG
Cuts at: 1606 2047 2214 2872 2900 3028 3950 4588 4669
Size: 441 167 658 28 128 922 638 81
Cuts at: 4669 6391 6669 8031 8708 9856 10101 1606
Size: 1722 278 1362 677 1148 245 2120
Fragments arranged by size:
2120 1722 1362 1148 922 677 658 638
441 278 245 167 128 81 28
NaeI GCC'GGC
Cuts at: 3735 4492 6403 6637 3735
Size: 757 1911 234 7713
Fragments arranged by size:
7713 1911 757 234
NarI GG'CG_CC
Cuts at: 3249 6591 3249
Size: 3342 7273
NcoI C'CATG_G
Cuts at: 513 1065 2545 2952 3437 3561 4524 4605 5685
Size: 552 1480 407 485 124 963 81 1080
Cuts at: 5685 6531 513
Size: 846 4597
Fragments arranged by size:
4597 1480 1080 963 846 552 485 407
124 81
NdeI CA'TA_TG
Cuts at: 387 2462 387
Size: 2075 8540
NgoMIV G'CCGG_C
Cuts at: 3733 4490 6401 6635 3733
Size: 757 1911 234 7713
Fragments arranged by size:
7713 1911 757 234
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10615
NotI GC'GGCC_GC
Cuts at: 8062 8062
Size: 10615
NspI r_CATG'y
Cuts at: 1132 1229 2043 2226 2435 3355 5881 5932 6508
Size: 97 814 183 209 920 2526 51 576
Cuts at: 6508 7973 9518 1132
Size: 1465 1545 2229
Fragments arranged by size:
2526 2229 1545 1465 920 814 576 209
183 97 51
PasI CC'CwG_GG
Cuts at: 1348 4190 6626 1348
Size: 2842 2436 5337
Fragments arranged by size:
5337 2842 2436
PciI A'CATG_T
Cuts at: 2222 2431 3351 9514 2222
Size: 209 920 6163 3323
Fragments arranged by size:
6163 3323 920 209
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3556 4525 4720 6238 1108
Size: 2448 969 195 1518 5485
Fragments arranged by size:
5485 2448 1518 969 195
PfoI T'CCnGG_A
Cuts at: 3549 3666 6009 3549
Size: 117 2343 8155
Fragments arranged by size:
8155 2343 117
PmeI GTTT'AAAC
Cuts at: 7919 7919
Size: 10615
PmlI CAC'GTG
Cuts at: 2293 2293
Size: 10615
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4745 4777 5213 5245 8684 8716 10197 10229 4745
Size: 32 436 32 3439 32 1481 32 5131
Fragments arranged by size:
5131 3439 1481 436 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3724 4813 6723 6731 3724
Size: 1089 1910 8 7608
Fragments arranged by size:
7608 1910 1089 8
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10615
PsiI TTA'TAA
Cuts at: 8185 8185
Size: 10615
PspOMI G'GGCC_C
Cuts at: 4788 4788
Size: 10615
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 10583
PstI C_TGCA'G
Cuts at: 830 3327 4809 5901 6000 7967 830
Size: 2497 1482 1092 99 1967 3478
Fragments arranged by size:
3478 2497 1967 1482 1092 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10615
PvuII CAG'CTG
Cuts at: 2872 3950 4669 6391 2872
Size: 1078 719 1722 7096
Fragments arranged by size:
7096 1722 1078 719
SacI G_AGCT'C
Cuts at: 5385 5385
Size: 10615
SalI G'TCGA_C
Cuts at: 1936 3706 5094 7957 1936
Size: 1770 1388 2863 4594
Fragments arranged by size:
4594 2863 1770 1388
SanDI GG'GwC_CC
Cuts at: 6723 6723
Size: 10615
SapI GCTCTTCn'nnn_
Cuts at: 5129 5129
Size: 10615
SbfI CC_TGCA'GG
Cuts at: 5901 6000 7967 5901
Size: 99 1967 8549
Fragments arranged by size:
8549 1967 99
ScaI AGT'ACT
Cuts at: 7583 7625 7730 7772 8863 10575 7583
Size: 42 105 42 1091 1712 7623
Fragments arranged by size:
7623 1712 1091 105 42 42
SexAI A'CCwGG_T
Cuts at: 3132 3793 4656 4815 6757 3132
Size: 661 863 159 1942 6990
Fragments arranged by size:
6990 1942 863 661 159
SfcI C'TryA_G
Cuts at: 826 1045 3323 4805 5897 5996 6910 7288 7351
Size: 219 2278 1482 1092 99 914 378 63
Cuts at: 7351 7477 7498 7864 7963 9098 9779 9970 826
Size: 126 21 366 99 1135 681 191 1471
Fragments arranged by size:
2278 1482 1471 1135 1092 914 681 378
366 219 191 126 99 99 63 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 2259 2259
Size: 10615
SfoI GGC'GCC
Cuts at: 3250 6592 3250
Size: 3342 7273
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10615
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10615
SmaI CCC'GGG
Cuts at: 1872 5254 6720 7942 1872
Size: 3382 1466 1222 4545
Fragments arranged by size:
4545 3382 1466 1222
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3011 4730 5375 5448 6736 8719
Size: 197 926 1068 1719 645 73 1288 1983
Cuts at: 8719 9620 9882 820
Size: 901 262 1553
Fragments arranged by size:
1983 1719 1553 1288 1068 926 901 645
262 197 73
SnaBI TAC'GTA
Cuts at: 493 8406 493
Size: 7913 2702
SpeI A'CTAG_T
Cuts at: 152 3217 7319 152
Size: 3065 4102 3448
Fragments arranged by size:
4102 3448 3065
SphI G_CATG'C
Cuts at: 1132 2043 7973 1132
Size: 911 5930 3774
Fragments arranged by size:
5930 3774 911
SspI AAT'ATT
Cuts at: 5 52 7604 8539 5
Size: 47 7552 935 2081
Fragments arranged by size:
7552 2081 935 47
StuI AGG'CCT
Cuts at: 1517 5195 1517
Size: 3678 6937
StyI C'CwwG_G
Cuts at: 513 1065 2403 2545 2952 3437 3561 3882 4524
Size: 552 1338 142 407 485 124 321 642
Cuts at: 4524 4605 5287 5466 5685 6531 6560 8054 513
Size: 81 682 179 219 846 29 1494 3074
Fragments arranged by size:
3074 1494 1338 846 682 642 552 485
407 321 219 179 142 124 81 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3842 4516 5318 5351 5569 6524 6538
Size: 1255 2262 674 802 33 218 955 14
Cuts at: 6538 6940 6961 6994 7057 7150 7204 7225 7246
Size: 402 21 33 63 93 54 21 21
Cuts at: 7246 7297 7318 7402 7486 7528 7624 7666 8642
Size: 51 21 84 84 42 96 42 976
Cuts at: 8642 8659 8812 8997 9442 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2262 1498 1255 976 955 802 674 445
402 218 185 153 96 93 84 84
63 54 51 42 42 33 33 21
21 21 21 17 14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3255 5925 7518
Size: 274 80 33 51 1443 1278 2670 1593
Cuts at: 7518 7581 7623 7728 7770 8861 10573 96
Size: 63 42 105 42 1091 1712 138
Fragments arranged by size:
2670 1712 1593 1443 1278 1091 274 138
105 80 63 51 42 42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3389 3421 5126 5158 5462 5494 6146 6178 6556
Size: 32 1705 32 304 32 652 32 378
Cuts at: 6556 6588 10575 10607 3389
Size: 32 3987 32 3397
Fragments arranged by size:
3987 3397 1705 652 378 304 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10615
XbaI T'CTAG_A
Cuts at: 7951 7951
Size: 10615
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2356 2552 3889 4657 5029 6088 2356
Size: 196 1337 768 372 1059 6883
Fragments arranged by size:
6883 1337 1059 768 372 196
XhoI C'TCGA_G
Cuts at: 1943 5448 1943
Size: 3505 7110
XmaI C'CCGG_G
Cuts at: 1870 5252 6718 7940 1870
Size: 3382 1466 1222 4545
Fragments arranged by size:
4545 3382 1466 1222
XmnI GAAnn'nnTTC
Cuts at: 4760 4982 5321 8744 4760
Size: 222 339 3423 6631
Fragments arranged by size:
6631 3423 339 222
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3661 8423 276
Size: 53 83 186 936 2127 4762 2468
Fragments arranged by size:
4762 2468 2127 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI FseI FspAI
NruI NsiI PacI PspXI RsrII SacII SrfI SwaI