Restriction map for
pFN21AE3488
(Circular) (Six-base) MAPSORT of: pFN21AE3488.seq Check: 1897 from: 1 to: 10183
pFN21AE3488 10183 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7993 278
Size: 53 83 186 936 6457 2468
Fragments arranged by size:
6457 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2325 7526 1937
Size: 388 5201 4594
Fragments arranged by size:
5201 4594 388
Acc65I G'GTAC_C
Cuts at: 7504 7504
Size: 10183
AclI AA'CG_TT
Cuts at: 1563 8310 8683 1563
Size: 6747 373 3063
Fragments arranged by size:
6747 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3686 4775 5358 7290 7326 7427 7437 8246
Size: 2054 1089 583 1932 36 101 10 809
Cuts at: 8246 9630 9898 1632
Size: 1384 268 1917
Fragments arranged by size:
2054 1932 1917 1384 1089 809 583 268
101 36 10
AflII C'TTAA_G
Cuts at: 820 1017 4652 5528 5762 5789 820
Size: 197 3635 876 234 27 5214
Fragments arranged by size:
5214 3635 876 234 197 27
AflIII A'CryG_T
Cuts at: 1203 3726 5379 9082 10059 1203
Size: 2523 1653 3703 977 1327
Fragments arranged by size:
3703 2523 1653 1327 977
AgeI A'CCGG_T
Cuts at: 9014 9014
Size: 10183
AhdI GACnn_n'nnGTC
Cuts at: 8912 8912
Size: 10183
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2483 2733 3438 4734 1184
Size: 1299 250 705 1296 6633
Fragments arranged by size:
6633 1299 1296 705 250
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6129 6161 6129
Size: 32 10151
AlwNI CAG_nnn'CTG
Cuts at: 2633 2669 4340 7926 9498 2633
Size: 36 1671 3586 1572 3318
Fragments arranged by size:
3586 3318 1671 1572 36
ApaI G_GGCC'C
Cuts at: 3888 5190 6984 3888
Size: 1302 1794 7087
Fragments arranged by size:
7087 1794 1302
ApaLI G'TGCA_C
Cuts at: 3939 8240 9396 3939
Size: 4301 1156 4726
Fragments arranged by size:
4726 4301 1156
ApoI r'AATT_y
Cuts at: 1493 2759 3020 3212 3597 3678 5087 5198 5672
Size: 1266 261 192 385 81 1409 111 474
Cuts at: 5672 5684 5891 7492 7718 7870 9979 1493
Size: 12 207 1601 226 152 2109 1697
Fragments arranged by size:
2109 1697 1601 1409 1266 474 385 261
226 207 192 152 111 81 12
AseI AT'TA_AT
Cuts at: 160 1032 8737 160
Size: 872 7705 1606
Fragments arranged by size:
7705 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10183
AvaI C'yCGr_G
Cuts at: 1870 1943 2488 6384 7038 7508 1870
Size: 73 545 3896 654 470 4545
Fragments arranged by size:
4545 3896 654 545 470 73
BamHI G'GATC_C
Cuts at: 7513 7880 7513
Size: 367 9816
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2276 3806 5864 7504 8959
Size: 325 217 627 489 1530 2058 1640 1455
Cuts at: 8959 618
Size: 1842
Fragments arranged by size:
2058 1842 1640 1530 1455 627 489 325
217
BanII G_rGCy'C
Cuts at: 1391 3888 4259 5190 6984 7502 1391
Size: 2497 371 931 1794 518 4072
Fragments arranged by size:
4072 2497 1794 931 518 371
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2454 2556 3791 5024 5526 6025 6522
Size: 971 555 102 1235 1233 502 499 497
Cuts at: 6522 7152 928
Size: 630 3959
Fragments arranged by size:
3959 1235 1233 971 630 555 502 499
497 102
BbvCI CC'TCA_GC
Cuts at: 2030 2030
Size: 10183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 1998 2021 2032 3890 3924 6050
Size: 34 50 11 23 11 1858 34 2126
Cuts at: 6050 6084 8374 8408 1903
Size: 34 2290 34 3678
Fragments arranged by size:
3678 2290 2126 1858 50 34 34 34
34 23 11 11
BciVI GTATCCnnnnn_n'
Cuts at: 2132 4527 8075 9285 2132
Size: 2395 3548 1210 3030
Fragments arranged by size:
3548 3030 2395 1210
BclI T'GATC_A
Cuts at: 1548 4350 1548
Size: 2802 7381
BfrBI ATG'CAT
Cuts at: 4465 4465
Size: 10183
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3363 8794 136
Size: 107 122 71 631 605 1691 5431 1525
Fragments arranged by size:
5431 1691 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4090 6253 6465 10178 4090
Size: 2163 212 3713 4095
Fragments arranged by size:
4095 3713 2163 212
BlpI GC'TnA_GC
Cuts at: 5229 5473 7600 5229
Size: 244 2127 7812
Fragments arranged by size:
7812 2127 244
Bme1580I G_kGCm'C
Cuts at: 1790 3809 3888 3943 5190 6984 7476 8244 9400
Size: 2019 79 55 1247 1794 492 768 1156
Cuts at: 9400 1790
Size: 2573
Fragments arranged by size:
2573 2019 1794 1247 1156 768 492 79
55
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10183
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2274 4923 6647
Size: 416 53 83 399 24 857 2649 1724
Cuts at: 6647 7914 8872 442
Size: 1267 958 1753
Fragments arranged by size:
2649 1753 1724 1267 958 857 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10183
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3640 7165 3640
Size: 3525 6658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2115 2177 3057 4218 4296 4432 5199 6248
Size: 876 62 880 1161 78 136 767 1049
Cuts at: 6248 6267 6428 7086 7191 8843 1239
Size: 19 161 658 105 1652 2579
Fragments arranged by size:
2579 1652 1161 1049 880 876 767 658
161 136 105 78 62 19
Bpu10I CC'TnA_GC
Cuts at: 2030 3525 4695 6840 7200 2030
Size: 1495 1170 2145 360 5013
Fragments arranged by size:
5013 2145 1495 1170 360
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3245 4310 5540 6074 6923 8308 9173 9471 3245
Size: 1065 1230 534 849 1385 865 298 3957
Fragments arranged by size:
3957 1385 1230 1065 865 849 534 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6707 8846 882
Size: 617 5208 2139 2219
Fragments arranged by size:
5208 2219 2139 617
BsaAI yAC'GTr
Cuts at: 493 1204 5084 5417 6722 7974 493
Size: 711 3880 333 1305 1252 2702
Fragments arranged by size:
3880 2702 1305 1252 711 333
BsaBI GATnn'nnATC
Cuts at: 4089 7230 10177 4089
Size: 3141 2947 4095
Fragments arranged by size:
4095 3141 2947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6190 7990 8372 9007
Size: 53 83 186 936 4657 1800 382 635
Cuts at: 9007 275
Size: 1451
Fragments arranged by size:
4657 1800 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4587 8616 9014 9288 9435 9846 4587
Size: 4029 398 274 147 411 4924
Fragments arranged by size:
4924 4029 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3620 3650 5951 5981 6333
Size: 30 165 30 2217 30 2301 30 352
Cuts at: 6333 6363 7084 7114 1178
Size: 30 721 30 4247
Fragments arranged by size:
4247 2301 2217 721 352 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2463 5441 5975 5978 7196 7199 1186
Size: 1277 2978 534 3 1218 3 4170
Fragments arranged by size:
4170 2978 1277 1218 534 3 3
BseYI C'CCAG_C
Cuts at: 2221 2699 3118 3498 5127 7135 7331 9386 9882
Size: 478 419 380 1629 2008 196 2055 496
Cuts at: 9882 2221
Size: 2522
Fragments arranged by size:
2522 2055 2008 1629 496 478 419 380
196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 9610
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7633 8394 9422 1540
Size: 275 186 5632 761 1028 2301
Fragments arranged by size:
5632 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2839 3943 4204 4312 8244 8329 9400 2839
Size: 1104 261 108 3932 85 1071 3622
Fragments arranged by size:
3932 3622 1104 1071 261 108 85
BsmI GAATG_Cn'
Cuts at: 2903 3832 5460 7694 7787 2903
Size: 929 1628 2234 93 5299
Fragments arranged by size:
5299 2234 1628 929 93
BsmBI CGTCTCn'nnnn_
Cuts at: 7041 7041
Size: 10183
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2839 3809 3888 3943 4204 4259 4312
Size: 399 1049 970 79 55 261 55 53
Cuts at: 4312 5190 6984 7476 7502 8244 8329 9400 1391
Size: 878 1794 492 26 742 85 1071 2174
Fragments arranged by size:
2174 1794 1071 1049 970 878 742 492
399 261 85 79 55 55 53 26
BspEI T'CCGG_A
Cuts at: 4587 9846 4587
Size: 5259 4924
BspHI T'CATG_A
Cuts at: 8072 9802 8072
Size: 1730 8453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4129 7538 844
Size: 3285 3409 3489
Fragments arranged by size:
3489 3409 3285
BsrBI CCG'CTC
Cuts at: 1394 1823 2147 5437 8070 1394
Size: 429 324 3290 2633 3507
Fragments arranged by size:
3507 3290 2633 429 324
BsrDI GCAATG_nn'
Cuts at: 65 1248 2571 3241 4875 8678 8852 65
Size: 1183 1323 670 1634 3803 174 1396
Fragments arranged by size:
3803 1634 1396 1323 1183 670 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8827 9014 1700
Size: 110 7017 187 2869
Fragments arranged by size:
7017 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 6917 7258 96
Size: 6821 341 3021
Fragments arranged by size:
6821 3021 341
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10183
BssSI C'ACGA_G
Cuts at: 8243 9255 8243
Size: 1012 9171
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5864 794
Size: 5070 5113
BstBI TT'CG_AA
Cuts at: 5930 8987 5930
Size: 3057 7126
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10183
BstXI CCAn_nnnn'nTGG
Cuts at: 2826 3035 3880 6636 2826
Size: 209 845 2756 6373
Fragments arranged by size:
6373 2756 845 209
BstYI r'GATC_y
Cuts at: 1972 2883 3481 3748 4069 4090 4192 4924 6253
Size: 911 598 267 321 21 102 732 1329
Cuts at: 6253 6465 6484 7513 7880 8265 8282 9734 9820
Size: 212 19 1029 367 385 17 1452 86
Cuts at: 9820 9832 10178 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1329 1029 911 732 598 385
367 346 321 267 212 102 86 21
19 17 12
Bsu36I CC'TnA_GG
Cuts at: 2646 3345 4030 4065 5100 2646
Size: 699 685 35 1035 7729
Fragments arranged by size:
7729 1035 699 685 35
BtgI C'CryG_G
Cuts at: 513 1065 3752 4526 5206 513
Size: 552 2687 774 680 5490
Fragments arranged by size:
5490 2687 774 680 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3893 487
Size: 683 56 2667 6777
Fragments arranged by size:
6777 2667 683 56
BtsI GCAGTG_nn'
Cuts at: 2049 2978 3145 4421 4718 5016 7092 7699 8493
Size: 929 167 1276 297 298 2076 607 794
Cuts at: 8493 8513 2049
Size: 20 3719
Fragments arranged by size:
3719 2076 1276 929 794 607 298 297
167 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10183
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2122 2157 529
Size: 35 1558 35 8555
Fragments arranged by size:
8555 1558 35 35
DraI TTT'AAA
Cuts at: 7487 7487
Size: 10183
DraIII CAC_nnn'GTG
Cuts at: 4935 6353 4935
Size: 1418 8765
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6602 6819 9190 809
Size: 507 5286 217 2371 1802
Fragments arranged by size:
5286 2371 1802 507 217
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4270 4408 4523
Size: 54 1425 50 275 18 2440 138 115
Cuts at: 4523 6329 6638 7630 8519 10148 8
Size: 1806 309 992 889 1629 43
Fragments arranged by size:
2440 1806 1629 1425 992 889 309 275
138 115 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7630 1537
Size: 275 5818 4090
Fragments arranged by size:
5818 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2871 3609 3665 4505 4586 4677 5100 5577
Size: 1518 738 56 840 81 91 423 477
Cuts at: 5577 5709 5946 6384 7239 8113 1353
Size: 132 237 438 855 874 3423
Fragments arranged by size:
3423 1518 874 855 840 738 477 438
423 237 132 91 81 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2845 5749 8770 9144 9290 387
Size: 1064 138 1256 2904 3021 374 146 1280
Fragments arranged by size:
3021 2904 1280 1256 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2177 3057 3686 4218 4296 4432
Size: 393 483 62 880 629 532 78 136
Cuts at: 4432 4775 5199 5358 6248 6267 6428 7086 7191
Size: 343 424 159 890 19 161 658 105
Cuts at: 7191 7290 7326 7427 7437 8246 8843 9630 9898
Size: 99 36 101 10 809 597 787 268
Cuts at: 9898 1239
Size: 1524
Fragments arranged by size:
1524 890 880 809 787 658 629 597
532 483 424 393 343 268 161 159
136 105 101 99 78 62 36 19
10
EcoNI CCTnn'n_nnAGG
Cuts at: 7286 7286
Size: 10183
EcoO109I rG'GnC_Cy
Cuts at: 2642 3884 3956 4869 5186 5795 6980 6981 2642
Size: 1242 72 913 317 609 1185 1 5844
Fragments arranged by size:
5844 1242 1185 913 609 317 72 1
EcoRI G'AATT_C
Cuts at: 3678 7492 7870 3678
Size: 3814 378 5991
Fragments arranged by size:
5991 3814 378
EcoRV GAT'ATC
Cuts at: 3322 3322
Size: 10183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 10183
FspI TGC'GCA
Cuts at: 4058 5150 5648 8689 4058
Size: 1092 498 3041 5552
Fragments arranged by size:
5552 3041 1092 498
FspAI rTGC'GCAy
Cuts at: 4058 5150 5648 4058
Size: 1092 498 8593
Fragments arranged by size:
8593 1092 498
HaeII r_GCGC'y
Cuts at: 1678 9330 1678
Size: 7652 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2458 2490 2538 2570 2615
Size: 32 135 32 886 32 48 32 45
Cuts at: 2615 2629 2647 2661 3328 3360 4504 4536 5294
Size: 14 18 14 667 32 1144 32 758
Cuts at: 5294 5315 5326 5347 5490 5522 5538 5570 6682
Size: 21 11 21 143 32 16 32 1112
Cuts at: 6682 6714 8824 8856 8898 8930 1373
Size: 32 2110 32 42 32 2626
Fragments arranged by size:
2626 2110 1144 1112 886 758 667 143
135 48 45 42 32 32 32 32
32 32 32 32 32 32 32 21
21 18 16 14 14 11
HincII GTy'rAC
Cuts at: 669 1657 1938 5160 7527 7773 669
Size: 988 281 3222 2367 246 3079
Fragments arranged by size:
3222 3079 2367 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5786 748
Size: 5038 5145
HpaI GTT'AAC
Cuts at: 7773 7773
Size: 10183
KpnI G_GTAC'C
Cuts at: 7508 7508
Size: 10183
MfeI C'AATT_G
Cuts at: 7782 7782
Size: 10183
MluI A'CGCG_T
Cuts at: 10059 10059
Size: 10183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3525 3994 5949 6115 9297 9481 1305
Size: 2220 469 1955 166 3182 184 2007
Fragments arranged by size:
3182 2220 2007 1955 469 184 166
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4272 4410 6640 10
Size: 54 1425 343 2440 138 2230 3553
Fragments arranged by size:
3553 2440 2230 1425 343 138 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2999 3878 4628 5419 5499 7467 8141
Size: 648 1833 879 750 791 80 1968 674
Cuts at: 8141 8500 8659 518
Size: 359 159 2042
Fragments arranged by size:
2042 1968 1833 879 791 750 674 648
359 159 80
MspA1I CmG'CkG
Cuts at: 1606 2155 3283 5127 5336 5410 5627 7375 7599
Size: 549 1128 1844 209 74 217 1748 224
Cuts at: 7599 8276 9424 9669 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1844 1748 1148 1128 677 549 245
224 217 209 74
NcoI C'CATG_G
Cuts at: 513 1065 3752 513
Size: 552 2687 6944
Fragments arranged by size:
6944 2687 552
NdeI CA'TA_TG
Cuts at: 387 5877 387
Size: 5490 4693
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10183
NotI GC'GGCC_GC
Cuts at: 7630 7630
Size: 10183
NsiI A_TGCA'T
Cuts at: 4467 4467
Size: 10183
NspI r_CATG'y
Cuts at: 1132 1229 3730 5383 5862 7451 7541 9086 1132
Size: 97 2501 1653 479 1589 90 1545 2229
Fragments arranged by size:
2501 2229 1653 1589 1545 479 97 90
PasI CC'CwG_GG
Cuts at: 1348 6977 1348
Size: 5629 4554
PciI A'CATG_T
Cuts at: 3726 5379 9082 3726
Size: 1653 3703 4827
Fragments arranged by size:
4827 3703 1653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2699 2893 1108
Size: 1591 194 8398
Fragments arranged by size:
8398 1591 194
PfoI T'CCnGG_A
Cuts at: 2886 4195 6225 2886
Size: 1309 2030 6844
Fragments arranged by size:
6844 2030 1309
PmeI GTTT'AAAC
Cuts at: 7487 7487
Size: 10183
PmlI CAC'GTG
Cuts at: 5417 5417
Size: 10183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8252 8284 9765 9797 8252
Size: 32 1481 32 8638
Fragments arranged by size:
8638 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2642 3956 5795 2642
Size: 1314 1839 7030
Fragments arranged by size:
7030 1839 1314
PshAI GACnn'nnGTC
Cuts at: 1613 5046 1613
Size: 3433 6750
PsiI TTA'TAA
Cuts at: 4173 7753 4173
Size: 3580 6603
PspOMI G'GGCC_C
Cuts at: 3884 5186 6980 3884
Size: 1302 1794 7087
Fragments arranged by size:
7087 1794 1302
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6845 6877 6845
Size: 32 10151
PstI C_TGCA'G
Cuts at: 830 4284 7535 830
Size: 3454 3251 3478
Fragments arranged by size:
3478 3454 3251
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10183
PvuII CAG'CTG
Cuts at: 2155 3283 5336 5410 5627 7375 2155
Size: 1128 2053 74 217 1748 4963
Fragments arranged by size:
4963 2053 1748 1128 217 74
SalI G'TCGA_C
Cuts at: 1936 7525 1936
Size: 5589 4594
SapI GCTCTTCn'nnn_
Cuts at: 2871 5946 2871
Size: 3075 7108
SbfI CC_TGCA'GG
Cuts at: 7535 7535
Size: 10183
ScaI AGT'ACT
Cuts at: 3929 8431 10143 3929
Size: 4502 1712 3969
Fragments arranged by size:
4502 3969 1712
SfcI C'TryA_G
Cuts at: 826 1045 2322 4280 4481 5276 7531 8666 9347
Size: 219 1277 1958 201 795 2255 1135 681
Cuts at: 9347 9538 826
Size: 191 1471
Fragments arranged by size:
2255 1958 1471 1277 1135 795 681 219
201 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10183
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10183
SmaI CCC'GGG
Cuts at: 1872 2490 7510 1872
Size: 618 5020 4545
Fragments arranged by size:
5020 4545 618
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3224 4289 4652 5528 5555 5762
Size: 197 926 1281 1065 363 876 27 207
Cuts at: 5762 5789 6089 6902 7038 8287 9188 9450 820
Size: 27 300 813 136 1249 901 262 1553
Fragments arranged by size:
1553 1281 1249 1065 926 901 876 813
363 300 262 207 197 136 27 27
SnaBI TAC'GTA
Cuts at: 493 5084 6722 7974 493
Size: 4591 1638 1252 2702
Fragments arranged by size:
4591 2702 1638 1252
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10183
SphI G_CATG'C
Cuts at: 1132 7451 7541 1132
Size: 6319 90 3774
Fragments arranged by size:
6319 3774 90
SspI AAT'ATT
Cuts at: 5 52 3823 8107 5
Size: 47 3771 4284 2081
Fragments arranged by size:
4284 3771 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 10183
StyI C'CwwG_G
Cuts at: 513 1065 2280 3449 3639 3752 4853 7126 7622
Size: 552 1215 1169 190 113 1101 2273 496
Cuts at: 7622 513
Size: 3074
Fragments arranged by size:
3074 2273 1215 1169 1101 552 496 190
113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3110 3442 3890 4636 6623 6666 7127
Size: 1255 1530 332 448 746 1987 43 461
Cuts at: 7127 8210 8227 8380 8565 9010 325
Size: 1083 17 153 185 445 1498
Fragments arranged by size:
1987 1530 1498 1255 1083 746 461 448
445 332 185 153 43 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2309 3927 6825
Size: 274 80 33 51 1443 332 1618 2898
Cuts at: 6825 6917 7258 8429 10141 96
Size: 92 341 1171 1712 138
Fragments arranged by size:
2898 1712 1618 1443 1171 341 332 274
138 92 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2168 2200 2260 2292 6258 6290 7084 7116 10143
Size: 32 60 32 3966 32 794 32 3027
Cuts at: 10143 10175 2168
Size: 32 2176
Fragments arranged by size:
3966 3027 2176 794 60 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5550 6214 1316
Size: 4234 664 5285
Fragments arranged by size:
5285 4234 664
XbaI T'CTAG_A
Cuts at: 3197 6766 7519 3197
Size: 3569 753 5861
Fragments arranged by size:
5861 3569 753
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3070 3070
Size: 10183
XhoI C'TCGA_G
Cuts at: 1943 7038 1943
Size: 5095 5088
XmaI C'CCGG_G
Cuts at: 1870 2488 7508 1870
Size: 618 5020 4545
Fragments arranged by size:
5020 4545 618
XmnI GAAnn'nnTTC
Cuts at: 2751 8312 2751
Size: 5561 4622
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7991 276
Size: 53 83 186 936 6457 2468
Fragments arranged by size:
6457 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BsiWI BstZ17I
EcoICRI FseI KasI NaeI NarI NgoMIV NruI PacI
PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI