Restriction map for
pFN21AE3486
(Circular) (Six-base) MAPSORT of: pFN21AE3486.seq Check: 3055 from: 1 to: 10081
pFN21AE3486 10081 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7891 278
Size: 53 83 186 936 6355 2468
Fragments arranged by size:
6355 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3508 7424 1937
Size: 1571 3916 4594
Fragments arranged by size:
4594 3916 1571
Acc65I G'GTAC_C
Cuts at: 7402 7402
Size: 10081
AclI AA'CG_TT
Cuts at: 1563 8208 8581 1563
Size: 6645 373 3063
Fragments arranged by size:
6645 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2388 2403 5478 5799 5871 5946 6009 6960
Size: 756 15 3075 321 72 75 63 951
Cuts at: 6960 7284 8144 9528 9796 1632
Size: 324 860 1384 268 1917
Fragments arranged by size:
3075 1917 1384 951 860 756 324 321
268 75 72 63 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9884
AflIII A'CryG_T
Cuts at: 1203 2481 2483 2888 2897 4133 8980 9957 1203
Size: 1278 2 405 9 1236 4847 977 1327
Fragments arranged by size:
4847 1327 1278 1236 977 405 9 2
AgeI A'CCGG_T
Cuts at: 8912 8912
Size: 10081
AhdI GACnn_n'nnGTC
Cuts at: 8810 8810
Size: 10081
AleI CACnn'nnGTG
Cuts at: 1166 7265 1166
Size: 6099 3982
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5855 1184
Size: 4671 5410
AlwNI CAG_nnn'CTG
Cuts at: 2841 3227 3827 5009 7262 7824 9396 2841
Size: 386 600 1182 2253 562 1572 3526
Fragments arranged by size:
3526 2253 1572 1182 600 562 386
ApaLI G'TGCA_C
Cuts at: 8138 9294 8138
Size: 1156 8925
ApoI r'AATT_y
Cuts at: 1493 2024 2203 2226 2583 2942 3923 4618 5601
Size: 531 179 23 357 359 981 695 983
Cuts at: 5601 6120 6128 6319 7390 7616 7768 9877 1493
Size: 519 8 191 1071 226 152 2109 1697
Fragments arranged by size:
2109 1697 1071 983 981 695 531 519
359 357 226 191 179 152 23 8
AseI AT'TA_AT
Cuts at: 160 1032 8635 160
Size: 872 7603 1606
Fragments arranged by size:
7603 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10081
AvaI C'yCGr_G
Cuts at: 1870 1943 7406 1870
Size: 73 5463 4545
Fragments arranged by size:
5463 4545 73
AvrII C'CTAG_G
Cuts at: 2561 2561
Size: 10081
BamHI G'GATC_C
Cuts at: 5783 7411 7778 5783
Size: 1628 367 8086
Fragments arranged by size:
8086 1628 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3400 7402 8857 618
Size: 325 217 627 1613 4002 1455 1842
Fragments arranged by size:
4002 1842 1613 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2070 5822 7400 1391
Size: 679 3752 1578 4072
Fragments arranged by size:
4072 3752 1578 679
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3171 3204 4653 5213 5481 928
Size: 971 1272 33 1449 560 268 5528
Fragments arranged by size:
5528 1449 1272 971 560 268 33
BbvCI CC'TCA_GC
Cuts at: 4564 4564
Size: 10081
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2391 2425 8272 8306 1903
Size: 34 454 34 5847 34 3678
Fragments arranged by size:
5847 3678 454 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3262 3708 6099 7973 9183 3262
Size: 446 2391 1874 1210 4160
Fragments arranged by size:
4160 2391 1874 1210 446
BclI T'GATC_A
Cuts at: 1548 6634 1548
Size: 5086 4995
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2363 8692 136
Size: 107 122 71 631 605 691 6329 1525
Fragments arranged by size:
6329 1525 691 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3080 4121 4250 4316 5156 10076 3080
Size: 1041 129 66 840 4920 3085
Fragments arranged by size:
4920 3085 1041 840 129 66
BlpI GC'TnA_GC
Cuts at: 7162 7498 7162
Size: 336 9745
Bme1580I G_kGCm'C
Cuts at: 1790 8142 9298 1790
Size: 6352 1156 2573
Fragments arranged by size:
6352 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2898 6142 1318
Size: 1580 3244 5257
Fragments arranged by size:
5257 3244 1580
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5658 6825 6847
Size: 416 53 83 399 24 4241 1167 22
Cuts at: 6847 7812 8770 442
Size: 965 958 1753
Fragments arranged by size:
4241 1753 1167 965 958 416 399 83
53 24 22
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10081
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5178 5178
Size: 10081
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3204 3648 3792 3804 4566 4572 4761 5847
Size: 1965 444 144 12 762 6 189 1086
Cuts at: 5847 8741 1239
Size: 2894 2579
Fragments arranged by size:
2894 2579 1965 1086 762 444 189 144
12 6
Bpu10I CC'TnA_GC
Cuts at: 4002 4564 4002
Size: 562 9519
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3624 4308 4350 6598 8206 9071 9369 3624
Size: 684 42 2248 1608 865 298 4336
Fragments arranged by size:
4336 2248 1608 865 684 298 42
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3037 4455 8744 882
Size: 617 1538 1418 4289 2219
Fragments arranged by size:
4289 2219 1538 1418 617
BsaAI yAC'GTr
Cuts at: 493 1204 2484 7872 493
Size: 711 1280 5388 2702
Fragments arranged by size:
5388 2702 1280 711
BsaBI GATnn'nnATC
Cuts at: 4315 6693 10075 4315
Size: 2378 3382 4321
Fragments arranged by size:
4321 3382 2378
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7888 8270 8905 275
Size: 53 83 186 936 6355 382 635 1451
Fragments arranged by size:
6355 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 8514 8912 9186 9333 9744 8514
Size: 398 274 147 411 8851
Fragments arranged by size:
8851 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3708 3738 1178
Size: 30 165 30 2305 30 7521
Fragments arranged by size:
7521 2305 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2741 3006 3346 3595 3696 3732 3747 3795
Size: 1555 265 340 249 101 36 15 48
Cuts at: 3795 4311 5151 5210 1186
Size: 516 840 59 6057
Fragments arranged by size:
6057 1555 840 516 340 265 249 101
59 48 36 15
BseYI C'CCAG_C
Cuts at: 2340 3142 3516 3788 7091 7103 9284 9780 2340
Size: 802 374 272 3303 12 2181 496 2641
Fragments arranged by size:
3303 2641 2181 802 496 374 272 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3884 1163
Size: 573 2148 7360
Fragments arranged by size:
7360 2148 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7531 8292 9320 1540
Size: 275 186 5530 761 1028 2301
Fragments arranged by size:
5530 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2579 2886 3342 4552 5822 8142 8227 9298 2579
Size: 307 456 1210 1270 2320 85 1071 3362
Fragments arranged by size:
3362 2320 1270 1210 1071 456 307 85
BsmI GAATG_Cn'
Cuts at: 7592 7685 7592
Size: 93 9988
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2579 2886 3342 4552 5822 7400
Size: 399 280 509 307 456 1210 1270 1578
Cuts at: 7400 8142 8227 9298 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1578 1270 1210 1071 742 509 456
399 307 280 85
BspEI T'CCGG_A
Cuts at: 9744 9744
Size: 10081
BspHI T'CATG_A
Cuts at: 2598 6631 7970 9700 2598
Size: 4033 1339 1730 2979
Fragments arranged by size:
4033 2979 1730 1339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2148 5122 6046 7436 844
Size: 1304 2974 924 1390 3489
Fragments arranged by size:
3489 2974 1390 1304 924
BsrBI CCG'CTC
Cuts at: 1394 1823 3374 4167 7968 1394
Size: 429 1551 793 3801 3507
Fragments arranged by size:
3801 3507 1551 793 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2409 2640 8576 8750 65
Size: 1183 1161 231 5936 174 1396
Fragments arranged by size:
5936 1396 1183 1161 231 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8725 8912 1700
Size: 110 6915 187 2869
Fragments arranged by size:
6915 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10081
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10081
BssSI C'ACGA_G
Cuts at: 6381 8141 9153 6381
Size: 1760 1012 7309
Fragments arranged by size:
7309 1760 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3329 794
Size: 2535 7546
BstBI TT'CG_AA
Cuts at: 8885 8885
Size: 10081
BstEII G'GTnAC_C
Cuts at: 1184 3392 4191 4281 1184
Size: 2208 799 90 6984
Fragments arranged by size:
6984 2208 799 90
BstYI r'GATC_y
Cuts at: 1972 2614 3080 3103 4121 4250 4316 5078 5156
Size: 642 466 23 1018 129 66 762 78
Cuts at: 5156 5783 7411 7778 8163 8180 9632 9718 9730
Size: 627 1628 367 385 17 1452 86 12
Cuts at: 9730 10076 1972
Size: 346 1977
Fragments arranged by size:
1977 1628 1452 1018 762 642 627 466
385 367 346 129 86 78 66 23
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3604 513
Size: 552 2539 6990
Fragments arranged by size:
6990 2539 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2303 487
Size: 683 56 1077 8265
Fragments arranged by size:
8265 1077 683 56
BtsI GCAGTG_nn'
Cuts at: 7159 7597 8391 8411 7159
Size: 438 794 20 8829
Fragments arranged by size:
8829 794 438 20
ClaI AT'CG_AT
Cuts at: 1554 4267 1554
Size: 2713 7368
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3675 3710 5781 5816 529
Size: 35 3111 35 2071 35 4794
Fragments arranged by size:
4794 3111 2071 35 35 35
DraI TTT'AAA
Cuts at: 4999 6325 7385 4999
Size: 1326 1060 7695
Fragments arranged by size:
7695 1326 1060
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9088 809
Size: 507 7772 1802
Fragments arranged by size:
7772 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2419 3772 6662
Size: 54 1425 50 275 18 589 1353 2890
Cuts at: 6662 7528 8417 10046 8
Size: 866 889 1629 43
Fragments arranged by size:
2890 1629 1425 1353 889 866 589 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7528 1537
Size: 275 5716 4090
Fragments arranged by size:
5716 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2323 3378 3565 3948 4204 6549 6666 6960
Size: 970 1055 187 383 256 2345 117 294
Cuts at: 6960 8011 1353
Size: 1051 3423
Fragments arranged by size:
3423 2345 1055 1051 970 383 294 256
187 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3690 8668 9042 9188 387
Size: 1064 138 2101 4978 374 146 1280
Fragments arranged by size:
4978 2101 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5820 5820
Size: 10081
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2388 2403 3204 3648 3792 3804 4566
Size: 393 756 15 801 444 144 12 762
Cuts at: 4566 4572 4761 5478 5799 5847 5871 5946 6009
Size: 6 189 717 321 48 24 75 63
Cuts at: 6009 6960 7284 8144 8741 9528 9796 1239
Size: 951 324 860 597 787 268 1524
Fragments arranged by size:
1524 951 860 801 787 762 756 717
597 444 393 324 321 268 189 144
75 63 48 24 15 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2235 3807 5118 6573 2235
Size: 1572 1311 1455 5743
Fragments arranged by size:
5743 1572 1455 1311
EcoO109I rG'GnC_Cy
Cuts at: 5123 5583 6994 5123
Size: 460 1411 8210
Fragments arranged by size:
8210 1411 460
EcoRI G'AATT_C
Cuts at: 2203 2226 2583 3923 4618 7390 7768 2203
Size: 23 357 1340 695 2772 378 4516
Fragments arranged by size:
4516 2772 1340 695 378 357 23
EcoRV GAT'ATC
Cuts at: 3090 4262 3090
Size: 1172 8909
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3125 3125
Size: 10081
FspI TGC'GCA
Cuts at: 2157 8587 2157
Size: 6430 3651
HaeII r_GCGC'y
Cuts at: 1678 4048 9228 1678
Size: 2370 5180 2531
Fragments arranged by size:
5180 2531 2370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2344 2376 2717 2749 2887
Size: 32 135 32 772 32 341 32 138
Cuts at: 2887 2919 3067 3099 3441 3473 4970 5002 5345
Size: 32 148 32 342 32 1497 32 343
Cuts at: 5345 5377 5471 5503 5702 5734 5969 5990 6001
Size: 32 94 32 199 32 235 21 11
Cuts at: 6001 6022 6512 6544 6665 6697 8722 8754 8796
Size: 21 490 32 121 32 2025 32 42
Cuts at: 8796 8828 1373
Size: 32 2626
Fragments arranged by size:
2626 2025 1497 772 490 343 342 341
235 199 148 138 135 121 94 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 21
21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3509 4453 5715 7425 7671 669
Size: 988 281 1571 944 1262 1710 246 3079
Fragments arranged by size:
3079 1710 1571 1262 988 944 281 246
HindIII A'AGCT_T
Cuts at: 748 4706 748
Size: 3958 6123
HpaI GTT'AAC
Cuts at: 7671 7671
Size: 10081
KpnI G_GTAC'C
Cuts at: 7406 7406
Size: 10081
MfeI C'AATT_G
Cuts at: 2307 2691 3360 7680 2307
Size: 384 669 4320 4708
Fragments arranged by size:
4708 4320 669 384
MluI A'CGCG_T
Cuts at: 9957 9957
Size: 10081
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2918 3552 6216 9195 9379 1305
Size: 1613 634 2664 2979 184 2007
Fragments arranged by size:
2979 2664 2007 1613 634 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2421 3774 10
Size: 54 1425 343 589 1353 6317
Fragments arranged by size:
6317 1425 1353 589 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2907 7265 8039 8398 8557 518
Size: 648 1741 4358 774 359 159 2042
Fragments arranged by size:
4358 2042 1741 774 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3320 3788 5924 7497 8174 9322 9567 1606
Size: 1714 468 2136 1573 677 1148 245 2120
Fragments arranged by size:
2136 2120 1714 1573 1148 677 468 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 9529
NdeI CA'TA_TG
Cuts at: 387 2263 2686 4420 387
Size: 1876 423 1734 6048
Fragments arranged by size:
6048 1876 1734 423
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10081
NotI GC'GGCC_GC
Cuts at: 7528 7528
Size: 10081
NspI r_CATG'y
Cuts at: 1132 1229 2892 6722 7256 7439 8984 1132
Size: 97 1663 3830 534 183 1545 2229
Fragments arranged by size:
3830 2229 1663 1545 534 183 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10081
PciI A'CATG_T
Cuts at: 2888 8980 2888
Size: 6092 3989
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2534 3503 3788 4784 1108
Size: 1426 969 285 996 6405
Fragments arranged by size:
6405 1426 996 969 285
PfoI T'CCnGG_A
Cuts at: 2868 4777 2868
Size: 1909 8172
PmeI GTTT'AAAC
Cuts at: 7385 7385
Size: 10081
PmlI CAC'GTG
Cuts at: 2484 2484
Size: 10081
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8150 8182 9663 9695 8150
Size: 32 1481 32 8536
Fragments arranged by size:
8536 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5123 5583 6994 5123
Size: 460 1411 8210
Fragments arranged by size:
8210 1411 460
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10081
PsiI TTA'TAA
Cuts at: 2022 2270 4530 6050 7651 2022
Size: 248 2260 1520 1601 4452
Fragments arranged by size:
4452 2260 1601 1520 248
PstI C_TGCA'G
Cuts at: 830 2145 3325 7433 830
Size: 1315 1180 4108 3478
Fragments arranged by size:
4108 3478 1315 1180
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10081
PvuII CAG'CTG
Cuts at: 3320 5924 3320
Size: 2604 7477
SacI G_AGCT'C
Cuts at: 5822 5822
Size: 10081
SalI G'TCGA_C
Cuts at: 1936 3507 7423 1936
Size: 1571 3916 4594
Fragments arranged by size:
4594 3916 1571
SbfI CC_TGCA'GG
Cuts at: 7433 7433
Size: 10081
ScaI AGT'ACT
Cuts at: 6835 8329 10041 6835
Size: 1494 1712 6875
Fragments arranged by size:
6875 1712 1494
SexAI A'CCwGG_T
Cuts at: 3396 3396
Size: 10081
SfcI C'TryA_G
Cuts at: 826 1045 2141 2670 3321 3589 3928 4072 4893
Size: 219 1096 529 651 268 339 144 821
Cuts at: 4893 6684 7429 8564 9245 9436 826
Size: 1791 745 1135 681 191 1471
Fragments arranged by size:
1791 1471 1135 1096 821 745 681 651
529 339 268 219 191 144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10081
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10081
SmaI CCC'GGG
Cuts at: 1872 7408 1872
Size: 5536 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3639 4323 4365 6613 8185 9086
Size: 197 926 1696 684 42 2248 1572 901
Cuts at: 9086 9348 820
Size: 262 1553
Fragments arranged by size:
2248 1696 1572 1553 926 901 684 262
197 42
SnaBI TAC'GTA
Cuts at: 493 7872 493
Size: 7379 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10081
SphI G_CATG'C
Cuts at: 1132 7439 1132
Size: 6307 3774
SspI AAT'ATT
Cuts at: 5 52 3312 4388 4526 5104 8005 5
Size: 47 3260 1076 138 578 2901 2081
Fragments arranged by size:
3260 2901 2081 1076 578 138 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 10081
StyI C'CwwG_G
Cuts at: 513 1065 2561 3183 3845 6135 6548 7520 513
Size: 552 1496 622 662 2290 413 972 3074
Fragments arranged by size:
3074 2290 1496 972 662 622 552 413
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3156 8108 8125 8278 8463 8908 325
Size: 1255 1576 4952 17 153 185 445 1498
Fragments arranged by size:
4952 1576 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5666 6062 6833
Size: 274 80 33 51 1443 3689 396 771
Cuts at: 6833 8327 10039 96
Size: 1494 1712 138
Fragments arranged by size:
3689 1712 1494 1443 771 396 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2569 2601 3454 3486 3708 3740 5070 5102 10041
Size: 32 853 32 222 32 1330 32 4939
Cuts at: 10041 10073 2569
Size: 32 2577
Fragments arranged by size:
4939 2577 1330 853 222 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10081
XbaI T'CTAG_A
Cuts at: 4247 6869 7417 4247
Size: 2622 548 6911
Fragments arranged by size:
6911 2622 548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5794 7121 5794
Size: 1327 8754
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10081
XmaI C'CCGG_G
Cuts at: 1870 7406 1870
Size: 5536 4545
XmnI GAAnn'nnTTC
Cuts at: 6985 8210 6985
Size: 1225 8856
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7889 276
Size: 53 83 186 936 6355 2468
Fragments arranged by size:
6355 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI