Restriction map for
pFN21AE3481
(Circular) (Six-base) MAPSORT of: pFN21AE3481.seq Check: 4139 from: 1 to: 9694
pFN21AE3481 9694 bp
With 182 enzymes: SgfI *
March 5, 2012 16:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4525 4903 4525
Size: 378 9316
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3669 7504 278
Size: 53 83 186 936 2133 3835 2468
Fragments arranged by size:
3835 2468 2133 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4752 4989 6441 7037 1937
Size: 2815 237 1452 596 4594
Fragments arranged by size:
4594 2815 1452 596 237
Acc65I G'GTAC_C
Cuts at: 2388 6177 7015 2388
Size: 3789 838 5067
Fragments arranged by size:
5067 3789 838
AclI AA'CG_TT
Cuts at: 1563 4326 6993 7821 8194 1563
Size: 2763 2667 828 373 3063
Fragments arranged by size:
3063 2763 2667 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3773 3968 4811 5024 5164 5630 6027 6278
Size: 2141 195 843 213 140 466 397 251
Cuts at: 6278 6806 7757 9141 9409 1632
Size: 528 951 1384 268 1917
Fragments arranged by size:
2141 1917 1384 951 843 528 466 397
268 251 213 195 140
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9497
AflIII A'CryG_T
Cuts at: 1203 2979 8593 9570 1203
Size: 1776 5614 977 1327
Fragments arranged by size:
5614 1776 1327 977
AgeI A'CCGG_T
Cuts at: 8525 8525
Size: 9694
AhdI GACnn_n'nnGTC
Cuts at: 2542 4927 8423 2542
Size: 2385 3496 3813
Fragments arranged by size:
3813 3496 2385
AleI CACnn'nnGTG
Cuts at: 1166 6884 1166
Size: 5718 3976
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3290 1184
Size: 2106 7588
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4274 4306 4274
Size: 32 9662
AlwNI CAG_nnn'CTG
Cuts at: 2171 3334 3544 5173 5232 5878 7437 9009 2171
Size: 1163 210 1629 59 646 1559 1572 2856
Fragments arranged by size:
2856 1629 1572 1559 1163 646 210 59
ApaI G_GGCC'C
Cuts at: 3778 3778
Size: 9694
ApaLI G'TGCA_C
Cuts at: 7751 8907 7751
Size: 1156 8538
ApoI r'AATT_y
Cuts at: 1493 2726 4941 5630 6083 7003 7229 7381 9490
Size: 1233 2215 689 453 920 226 152 2109
Cuts at: 9490 1493
Size: 1697
Fragments arranged by size:
2215 2109 1697 1233 920 689 453 226
152
AseI AT'TA_AT
Cuts at: 160 1032 8248 160
Size: 872 7216 1606
Fragments arranged by size:
7216 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9694
AvaI C'yCGr_G
Cuts at: 1870 1943 2893 2946 3404 3777 3889 5026 6899
Size: 73 950 53 458 373 112 1137 1873
Cuts at: 6899 7019 1870
Size: 120 4545
Fragments arranged by size:
4545 1873 1137 950 458 373 120 112
73 53
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5776 5809 5776
Size: 33 9661
BamHI G'GATC_C
Cuts at: 2826 3039 4846 7024 7391 2826
Size: 213 1807 2178 367 5129
Fragments arranged by size:
5129 2178 1807 367 213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2388 3620 5221 6023 6177
Size: 325 217 627 601 1232 1601 802 154
Cuts at: 6177 6953 6984 7015 8470 618
Size: 776 31 31 1455 1842
Fragments arranged by size:
1842 1601 1455 1232 802 776 627 601
325 217 154 31 31
BanII G_rGCy'C
Cuts at: 1391 3297 3399 3534 3689 3778 4584 5148 7013
Size: 1906 102 135 155 89 806 564 1865
Cuts at: 7013 1391
Size: 4072
Fragments arranged by size:
4072 1906 1865 806 564 155 135 102
89
BbeI G_GCGC'C
Cuts at: 6957 6957
Size: 9694
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2228 2538 2647 2961 3723 3910 4165
Size: 971 329 310 109 314 762 187 255
Cuts at: 4165 5251 5347 928
Size: 1086 96 5275
Fragments arranged by size:
5275 1086 971 762 329 314 310 255
187 109 96
BbvCI CC'TCA_GC
Cuts at: 6546 6857 6546
Size: 311 9383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2299 2333 7885 7919 1903
Size: 34 362 34 5552 34 3678
Fragments arranged by size:
5552 3678 362 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5945 7586 8796 5945
Size: 1641 1210 6843
Fragments arranged by size:
6843 1641 1210
BclI T'GATC_A
Cuts at: 1548 3483 1548
Size: 1935 7759
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3455 3974 4236
Size: 107 122 71 631 605 1783 519 262
Cuts at: 4236 5204 5435 5502 5717 8305 136
Size: 968 231 67 215 2588 1525
Fragments arranged by size:
2588 1783 1525 968 631 605 519 262
231 215 122 107 71 67
BglII A'GATC_T
Cuts at: 3849 6165 9689 3849
Size: 2316 3524 3854
Fragments arranged by size:
3854 3524 2316
BlpI GC'TnA_GC
Cuts at: 2909 7111 2909
Size: 4202 5492
Bme1580I G_kGCm'C
Cuts at: 1790 3778 3992 5224 6989 7755 8911 1790
Size: 1988 214 1232 1765 766 1156 2573
Fragments arranged by size:
2573 1988 1765 1232 1156 766 214
BmgBI CAC'GTC
Cuts at: 1318 3859 1318
Size: 2541 7153
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2688 4955 5950
Size: 416 53 83 399 24 1271 2267 995
Cuts at: 5950 7425 8383 442
Size: 1475 958 1753
Fragments arranged by size:
2267 1753 1475 1271 995 958 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9694
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2908 4571 2908
Size: 1663 8031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2156 2777 3135 5351 6225 6447 6479
Size: 893 24 621 358 2216 874 222 32
Cuts at: 6479 6812 8354 1239
Size: 333 1542 2579
Fragments arranged by size:
2579 2216 1542 893 874 621 358 333
222 32 24
Bpu10I CC'TnA_GC
Cuts at: 6054 6546 6857 6054
Size: 492 311 8891
Fragments arranged by size:
8891 492 311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2852 3283 4568 6077 6182 7819 8684 8982 2852
Size: 431 1285 1509 105 1637 865 298 3564
Fragments arranged by size:
3564 1637 1509 1285 865 431 298 105
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2117 3404 4928 5966 6180 6361 6827
Size: 617 618 1287 1524 1038 214 181 466
Cuts at: 6827 8357 882
Size: 1530 2219
Fragments arranged by size:
2219 1530 1524 1287 1038 618 617 466
214 181
BsaAI yAC'GTr
Cuts at: 493 1204 2686 2980 3389 4407 5248 7485 493
Size: 711 1482 294 409 1018 841 2237 2702
Fragments arranged by size:
2702 2237 1482 1018 841 711 409 294
BsaBI GATnn'nnATC
Cuts at: 3044 9688 3044
Size: 6644 3050
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2565 3517 3666 4012
Size: 53 83 186 936 1032 952 149 346
Cuts at: 4012 4645 5060 5859 6954 7501 7883 8518 275
Size: 633 415 799 1095 547 382 635 1451
Fragments arranged by size:
1451 1095 1032 952 936 799 635 633
547 415 382 346 186 149 83 53
BsaWI w'CCGG_w
Cuts at: 2590 6115 6487 8127 8525 8799 8946 9357 2590
Size: 3525 372 1640 398 274 147 411 2927
Fragments arranged by size:
3525 2927 1640 411 398 372 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2279 2309 3204 3234 1178
Size: 30 165 30 876 30 895 30 7638
Fragments arranged by size:
7638 895 876 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 5495 6563 6809 6817 6917 1186
Size: 949 3360 1068 246 8 100 3963
Fragments arranged by size:
3963 3360 1068 949 246 100 8
BseYI C'CCAG_C
Cuts at: 2024 2275 3248 5166 5189 5225 5661 5906 8897
Size: 251 973 1918 23 36 436 245 2991
Cuts at: 8897 9393 2024
Size: 496 2325
Fragments arranged by size:
2991 2325 1918 973 496 436 251 245
36 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2348 2957 3041 3215 3868 4043 4895
Size: 573 612 609 84 174 653 175 852
Cuts at: 4895 6752 6793 1163
Size: 1857 41 4064
Fragments arranged by size:
4064 1857 852 653 612 609 573 175
174 84 41
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7144 7905 8933 1540
Size: 275 186 5143 761 1028 2301
Fragments arranged by size:
5143 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3297 3399 4584 5148 6894 7755 7840 8911 3297
Size: 102 1185 564 1746 861 85 1071 4080
Fragments arranged by size:
4080 1746 1185 1071 861 564 102 85
BsmI GAATG_Cn'
Cuts at: 2874 3111 6917 7205 7298 2874
Size: 237 3806 288 93 5270
Fragments arranged by size:
5270 3806 288 237 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3297 3399 3534 3689 3778 3992 4584
Size: 399 1507 102 135 155 89 214 592
Cuts at: 4584 5148 5224 6894 6989 7013 7755 7840 8911
Size: 564 76 1670 95 24 742 85 1071
Cuts at: 8911 1391
Size: 2174
Fragments arranged by size:
2174 1670 1507 1071 742 592 564 399
214 155 135 102 95 89 85 76
24
BspEI T'CCGG_A
Cuts at: 2590 6487 9357 2590
Size: 3897 2870 2927
Fragments arranged by size:
3897 2927 2870
BspHI T'CATG_A
Cuts at: 3486 4374 7583 9313 3486
Size: 888 3209 1730 3867
Fragments arranged by size:
3867 3209 1730 888
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2641 3378 4509 4525 4903 5476 6724 7049
Size: 1797 737 1131 16 378 573 1248 325
Cuts at: 7049 844
Size: 3489
Fragments arranged by size:
3489 1797 1248 1131 737 573 378 325
16
BsrBI CCG'CTC
Cuts at: 1394 1823 2385 7581 1394
Size: 429 562 5196 3507
Fragments arranged by size:
5196 3507 562 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4269 5574 8189 8363 65
Size: 1183 3021 1305 2615 174 1396
Fragments arranged by size:
3021 2615 1396 1305 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3720 3911 4566 4718 8338 8525 1700
Size: 110 1910 191 655 152 3620 187 2869
Fragments arranged by size:
3620 2869 1910 655 191 187 152 110
BsrGI T'GTAC_A
Cuts at: 96 6636 96
Size: 6540 3154
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9694
BssSI C'ACGA_G
Cuts at: 3452 3943 4120 7754 8766 3452
Size: 491 177 3634 1012 4380
Fragments arranged by size:
4380 3634 1012 491 177
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3334 4083 794
Size: 2540 749 6405
Fragments arranged by size:
6405 2540 749
BstBI TT'CG_AA
Cuts at: 4525 8498 4525
Size: 3973 5721
BstEII G'GTnAC_C
Cuts at: 1184 2761 3764 6097 6583 1184
Size: 1577 1003 2333 486 4295
Fragments arranged by size:
4295 2333 1577 1003 486
BstXI CCAn_nnnn'nTGG
Cuts at: 2134 2251 3215 4234 4314 5824 2134
Size: 117 964 1019 80 1510 6004
Fragments arranged by size:
6004 1510 1019 964 117 80
BstYI r'GATC_y
Cuts at: 1972 2448 2619 2826 3039 3066 3462 3849 4446
Size: 476 171 207 213 27 396 387 597
Cuts at: 4446 4846 5558 6165 7024 7391 7776 7793 9245
Size: 400 712 607 859 367 385 17 1452
Cuts at: 9245 9331 9343 9689 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 859 712 607 597 476 400
396 387 385 367 346 213 207 171
86 27 17 12
Bsu36I CC'TnA_GG
Cuts at: 2017 6135 2017
Size: 4118 5576
BtgI C'CryG_G
Cuts at: 513 1065 2004 2127 2478 3168 3348 3769 3795
Size: 552 939 123 351 690 180 421 26
Cuts at: 3795 5064 513
Size: 1269 5143
Fragments arranged by size:
5143 1269 939 690 552 421 351 180
123 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5056 5266 487
Size: 683 56 3830 210 4915
Fragments arranged by size:
4915 3830 683 210 56
BtsI GCAGTG_nn'
Cuts at: 3016 3901 3958 5108 7210 8004 8024 3016
Size: 885 57 1150 2102 794 20 4686
Fragments arranged by size:
4686 2102 1150 885 794 57 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9694
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2650 2685 529
Size: 35 2086 35 7538
Fragments arranged by size:
7538 2086 35 35
DraI TTT'AAA
Cuts at: 6998 6998
Size: 9694
DraIII CAC_nnn'GTG
Cuts at: 4407 4407
Size: 9694
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5297 6095 6712 8701 809
Size: 507 3981 798 617 1989 1802
Fragments arranged by size:
3981 1989 1802 798 617 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3222 3535 3628
Size: 54 1425 50 275 18 1392 313 93
Cuts at: 3628 3645 3706 3792 3955 4242 4629 5571 6267
Size: 17 61 86 163 287 387 942 696
Cuts at: 6267 6693 7141 8030 9659 8
Size: 426 448 889 1629 43
Fragments arranged by size:
1629 1425 1392 942 889 696 448 426
387 313 287 275 163 93 86 61
54 50 43 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 7141 1537
Size: 275 5329 4090
Fragments arranged by size:
5329 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2399 4272 5625 5964 6918 7624 1353
Size: 1046 1873 1353 339 954 706 3423
Fragments arranged by size:
3423 1873 1353 1046 954 706 339
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3581 8281 8655 8801 387
Size: 1064 138 1992 4700 374 146 1280
Fragments arranged by size:
4700 1992 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3295 3397 4582 5146 3295
Size: 102 1185 564 7843
Fragments arranged by size:
7843 1185 564 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2156 2777 3135 3773 3968 4811
Size: 393 500 24 621 358 638 195 843
Cuts at: 4811 5024 5164 5351 5630 6027 6225 6278 6447
Size: 213 140 187 279 397 198 53 169
Cuts at: 6447 6479 6806 6812 7757 8354 9141 9409 1239
Size: 32 327 6 945 597 787 268 1524
Fragments arranged by size:
1524 945 843 787 638 621 597 500
397 393 358 327 279 268 213 198
195 187 169 140 53 32 24 6
EcoNI CCTnn'n_nnAGG
Cuts at: 4154 4259 6350 4154
Size: 105 2091 7498
Fragments arranged by size:
7498 2091 105
EcoO109I rG'GnC_Cy
Cuts at: 2009 2021 2343 2752 3541 3774 4956 5136 5615
Size: 12 322 409 789 233 1182 180 479
Cuts at: 5615 5668 6946 2009
Size: 53 1278 4757
Fragments arranged by size:
4757 1278 1182 789 479 409 322 233
180 53 12
EcoRI G'AATT_C
Cuts at: 4941 7003 7381 4941
Size: 2062 378 7254
Fragments arranged by size:
7254 2062 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4189 4189
Size: 9694
FspI TGC'GCA
Cuts at: 3278 4076 8200 3278
Size: 798 4124 4772
Fragments arranged by size:
4772 4124 798
FspAI rTGC'GCAy
Cuts at: 3278 3278
Size: 9694
HaeII r_GCGC'y
Cuts at: 1678 4132 6957 8841 1678
Size: 2454 2825 1884 2531
Fragments arranged by size:
2825 2531 2454 1884
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2279 2311 2528 2560 4913
Size: 32 135 32 707 32 217 32 2353
Cuts at: 4913 4945 5687 5719 6341 6373 8335 8367 8409
Size: 32 742 32 622 32 1962 32 42
Cuts at: 8409 8441 1373
Size: 32 2626
Fragments arranged by size:
2626 2353 1962 742 707 622 217 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2461 2500 2602 3424 4990 5275
Size: 988 281 523 39 102 822 1566 285
Cuts at: 5275 5491 6280 7038 7284 669
Size: 216 789 758 246 3079
Fragments arranged by size:
3079 1566 988 822 789 758 523 285
281 246 216 102 39
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9694
HpaI GTT'AAC
Cuts at: 7284 7284
Size: 9694
KasI G'GCGC_C
Cuts at: 6953 6953
Size: 9694
KpnI G_GTAC'C
Cuts at: 2392 6181 7019 2392
Size: 3789 838 5067
Fragments arranged by size:
5067 3789 838
MfeI C'AATT_G
Cuts at: 7293 7293
Size: 9694
MluI A'CGCG_T
Cuts at: 9570 9570
Size: 9694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3384 3948 4413 5923 6552 6718 8808 8992
Size: 2079 564 465 1510 629 166 2090 184
Cuts at: 8992 1305
Size: 2007
Fragments arranged by size:
2090 2079 2007 1510 629 564 465 184
166
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3224 3957 4244 4631 5573
Size: 54 1425 343 1392 733 287 387 942
Cuts at: 5573 6269 6695 10
Size: 696 426 3009
Fragments arranged by size:
3009 1425 1392 942 733 696 426 387
343 287 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3272 4478 5075 5822 6221 6884 7652
Size: 648 2106 1206 597 747 399 663 768
Cuts at: 7652 8011 8170 518
Size: 359 159 2042
Fragments arranged by size:
2106 2042 1206 768 747 663 648 597
399 359 159
MspA1I CmG'CkG
Cuts at: 1606 3036 3627 5173 5661 6005 6265 6540 6664
Size: 1430 591 1546 488 344 260 275 124
Cuts at: 6664 7110 7787 8935 9180 1606
Size: 446 677 1148 245 2120
Fragments arranged by size:
2120 1546 1430 1148 677 591 488 446
344 275 260 245 124
NarI GG'CG_CC
Cuts at: 6954 6954
Size: 9694
NcoI C'CATG_G
Cuts at: 513 1065 2004 2127 513
Size: 552 939 123 8080
Fragments arranged by size:
8080 939 552 123
NdeI CA'TA_TG
Cuts at: 387 4696 387
Size: 4309 5385
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9694
NotI GC'GGCC_GC
Cuts at: 7141 7141
Size: 9694
NspI r_CATG'y
Cuts at: 1132 1229 4099 4432 7052 8597 1132
Size: 97 2870 333 2620 1545 2229
Fragments arranged by size:
2870 2620 2229 1545 333 97
PasI CC'CwG_GG
Cuts at: 1348 3208 4150 1348
Size: 1860 942 6892
Fragments arranged by size:
6892 1860 942
PciI A'CATG_T
Cuts at: 8593 8593
Size: 9694
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2353 3544 5173 5232 5839 1108
Size: 1245 1191 1629 59 607 4963
Fragments arranged by size:
4963 1629 1245 1191 607 59
PfoI T'CCnGG_A
Cuts at: 2829 4176 4917 5328 5358 2829
Size: 1347 741 411 30 7165
Fragments arranged by size:
7165 1347 741 411 30
PmeI GTTT'AAAC
Cuts at: 6998 6998
Size: 9694
PmlI CAC'GTG
Cuts at: 4407 4407
Size: 9694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7763 7795 9276 9308 7763
Size: 32 1481 32 8149
Fragments arranged by size:
8149 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2009 2021 2343 3541 4956 5668 6946 2009
Size: 12 322 1198 1415 712 1278 4757
Fragments arranged by size:
4757 1415 1278 1198 712 322 12
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9694
PsiI TTA'TAA
Cuts at: 7264 7264
Size: 9694
PspOMI G'GGCC_C
Cuts at: 3774 3774
Size: 9694
PstI C_TGCA'G
Cuts at: 830 2382 4625 4728 4900 5460 5730 7046 830
Size: 1552 2243 103 172 560 270 1316 3478
Fragments arranged by size:
3478 2243 1552 1316 560 270 172 103
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9694
PvuII CAG'CTG
Cuts at: 5173 6005 6265 6664 5173
Size: 832 260 399 8203
Fragments arranged by size:
8203 832 399 260
SacI G_AGCT'C
Cuts at: 3297 3399 4584 5148 3297
Size: 102 1185 564 7843
Fragments arranged by size:
7843 1185 564 102
SalI G'TCGA_C
Cuts at: 1936 4988 7036 1936
Size: 3052 2048 4594
Fragments arranged by size:
4594 3052 2048
SanDI GG'GwC_CC
Cuts at: 2009 2343 4956 2009
Size: 334 2613 6747
Fragments arranged by size:
6747 2613 334
SbfI CC_TGCA'GG
Cuts at: 4728 7046 4728
Size: 2318 7376
ScaI AGT'ACT
Cuts at: 3092 7942 9654 3092
Size: 4850 1712 3132
Fragments arranged by size:
4850 3132 1712
SexAI A'CCwGG_T
Cuts at: 2958 3543 6173 2958
Size: 585 2630 6479
Fragments arranged by size:
6479 2630 585
SfcI C'TryA_G
Cuts at: 826 1045 2378 2718 3340 4621 4724 4896 5456
Size: 219 1333 340 622 1281 103 172 560
Cuts at: 5456 5552 5726 7042 8177 8858 9049 826
Size: 96 174 1316 1135 681 191 1471
Fragments arranged by size:
1471 1333 1316 1281 1135 681 622 560
340 219 191 174 172 103 96
SfiI GGCCn_nnn'nGGCC
Cuts at: 5435 5435
Size: 9694
SfoI GGC'GCC
Cuts at: 6955 6955
Size: 9694
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9694
SgrAI Cr'CCGG_yG
Cuts at: 1700 3911 1700
Size: 2211 7483
SmaI CCC'GGG
Cuts at: 1872 3406 3779 3891 7021 1872
Size: 1534 373 112 3130 4545
Fragments arranged by size:
4545 3130 1534 373 112
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2867 3262 4583 6092 6161 7798
Size: 197 926 924 395 1321 1509 69 1637
Cuts at: 7798 8699 8961 820
Size: 901 262 1553
Fragments arranged by size:
1637 1553 1509 1321 926 924 901 395
262 197 69
SnaBI TAC'GTA
Cuts at: 493 3389 7485 493
Size: 2896 4096 2702
Fragments arranged by size:
4096 2896 2702
SpeI A'CTAG_T
Cuts at: 152 2203 152
Size: 2051 7643
SphI G_CATG'C
Cuts at: 1132 4099 7052 1132
Size: 2967 2953 3774
Fragments arranged by size:
3774 2967 2953
SspI AAT'ATT
Cuts at: 5 52 7618 5
Size: 47 7566 2081
Fragments arranged by size:
7566 2081 47
StuI AGG'CCT
Cuts at: 1517 6133 6611 1517
Size: 4616 478 4600
Fragments arranged by size:
4616 4600 478
StyI C'CwwG_G
Cuts at: 513 1065 2004 2127 4065 5139 5838 5976 6019
Size: 552 939 123 1938 1074 699 138 43
Cuts at: 6019 7133 513
Size: 1114 3074
Fragments arranged by size:
3074 1938 1114 1074 939 699 552 138
123 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3100 3649 5180 5239 5942 6874 6972
Size: 1255 1520 549 1531 59 703 932 98
Cuts at: 6972 7721 7738 7891 8076 8521 325
Size: 749 17 153 185 445 1498
Fragments arranged by size:
1531 1520 1498 1255 932 749 703 549
445 185 153 98 59 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2079 3090 6636
Size: 274 80 33 51 1443 102 1011 3546
Cuts at: 6636 6684 7940 9652 96
Size: 48 1256 1712 138
Fragments arranged by size:
3546 1712 1443 1256 1011 274 138 102
80 51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2005 2037 3204 3236 3507 3539 4831 4863 9654
Size: 32 1167 32 271 32 1292 32 4791
Cuts at: 9654 9686 2005
Size: 32 2013
Fragments arranged by size:
4791 2013 1292 1167 271 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4049 4775 4913 5351 6353 6581 1316
Size: 2733 726 138 438 1002 228 4429
Fragments arranged by size:
4429 2733 1002 726 438 228 138
XbaI T'CTAG_A
Cuts at: 7030 7030
Size: 9694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2248 4252 5390 6301 2248
Size: 2004 1138 911 5641
Fragments arranged by size:
5641 2004 1138 911
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9694
XmaI C'CCGG_G
Cuts at: 1870 3404 3777 3889 7019 1870
Size: 1534 373 112 3130 4545
Fragments arranged by size:
4545 3130 1534 373 112
XmnI GAAnn'nnTTC
Cuts at: 4172 6557 7823 4172
Size: 2385 1266 6043
Fragments arranged by size:
6043 2385 1266
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3667 7502 276
Size: 53 83 186 936 2133 3835 2468
Fragments arranged by size:
3835 2468 2133 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BsiWI BsmBI BstZ17I EcoRV
FseI NaeI NgoMIV NruI NsiI PacI PspXI PsrI
RsrII SacII SapI SrfI SwaI