Restriction map for
pFN21AE3477
(Circular) (Six-base) MAPSORT of: pFN21AE3477.seq Check: 7347 from: 1 to: 9517
pFN21AE3477 9517 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7327 278
Size: 53 83 186 936 5791 2468
Fragments arranged by size:
5791 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6860 1937
Size: 4923 4594
Acc65I G'GTAC_C
Cuts at: 5941 6838 5941
Size: 897 8620
AclI AA'CG_TT
Cuts at: 1563 7644 8017 1563
Size: 6081 373 3063
Fragments arranged by size:
6081 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 3432 4580 7580 8964 9232 1632
Size: 476 1324 1148 3000 1384 268 1917
Fragments arranged by size:
3000 1917 1384 1324 1148 476 268
AfeI AGC'GCT
Cuts at: 6103 6103
Size: 9517
AflII C'TTAA_G
Cuts at: 820 1017 5849 820
Size: 197 4832 4488
Fragments arranged by size:
4832 4488 197
AflIII A'CryG_T
Cuts at: 1203 4688 8416 9393 1203
Size: 3485 3728 977 1327
Fragments arranged by size:
3728 3485 1327 977
AgeI A'CCGG_T
Cuts at: 8348 8348
Size: 9517
AhdI GACnn_n'nnGTC
Cuts at: 6784 8246 6784
Size: 1462 8055
AleI CACnn'nnGTG
Cuts at: 1166 5141 1166
Size: 3975 5542
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3487 6568 1184
Size: 2303 3081 4133
Fragments arranged by size:
4133 3081 2303
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5256 5288 6148 6180 5256
Size: 32 860 32 8593
Fragments arranged by size:
8593 860 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3425 3698 4276 5538 6639 7260 8832 3425
Size: 273 578 1262 1101 621 1572 4110
Fragments arranged by size:
4110 1572 1262 1101 621 578 273
ApaLI G'TGCA_C
Cuts at: 7574 8730 7574
Size: 1156 8361
ApoI r'AATT_y
Cuts at: 1493 2813 3383 3954 4992 5903 6564 6826 7052
Size: 1320 570 571 1038 911 661 262 226
Cuts at: 7052 7204 9313 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1320 1038 911 661 571 570
262 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8071 160
Size: 872 7039 1606
Fragments arranged by size:
7039 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9517
AvaI C'yCGr_G
Cuts at: 1870 1943 5910 6842 1870
Size: 73 3967 932 4545
Fragments arranged by size:
4545 3967 932 73
BamHI G'GATC_C
Cuts at: 5602 6847 7214 5602
Size: 1245 367 7905
Fragments arranged by size:
7905 1245 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 5511 5941 6838 8293
Size: 325 217 627 222 3502 430 897 1455
Cuts at: 8293 618
Size: 1842
Fragments arranged by size:
3502 1842 1455 897 627 430 325 222
217
BanII G_rGCy'C
Cuts at: 1391 5399 5700 6000 6009 6836 1391
Size: 4008 301 300 9 827 4072
Fragments arranged by size:
4072 4008 827 301 300 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3021 4505 5565 6492 6783 928
Size: 971 1122 1484 1060 927 291 3662
Fragments arranged by size:
3662 1484 1122 1060 971 927 291
BbvCI CC'TCA_GC
Cuts at: 2036 2054 2187 2036
Size: 18 133 9366
Fragments arranged by size:
9366 133 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7708 7742 1903
Size: 34 5771 34 3678
Fragments arranged by size:
5771 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5392 7409 8619 5392
Size: 2017 1210 6290
Fragments arranged by size:
6290 2017 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9517
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8128 136
Size: 107 122 71 631 605 6456 1525
Fragments arranged by size:
6456 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3024 4522 4667 9512 3024
Size: 1498 145 4845 3029
Fragments arranged by size:
4845 3029 1498 145
BlpI GC'TnA_GC
Cuts at: 5794 6934 5794
Size: 1140 8377
Bme1580I G_kGCm'C
Cuts at: 1790 5516 7578 8734 1790
Size: 3726 2062 1156 2573
Fragments arranged by size:
3726 2573 2062 1156
BmgBI CAC'GTC
Cuts at: 1318 6070 1318
Size: 4752 4765
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4594 7248 8206
Size: 416 53 83 399 24 3177 2654 958
Cuts at: 8206 442
Size: 1753
Fragments arranged by size:
3177 2654 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9517
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4413 4413
Size: 9517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2520 2583 2842 3049 5839 6154 8177 1239
Size: 1281 63 259 207 2790 315 2023 2579
Fragments arranged by size:
2790 2579 2023 1281 315 259 207 63
Bpu10I CC'TnA_GC
Cuts at: 2036 2054 2187 6077 2036
Size: 18 133 3890 5476
Fragments arranged by size:
5476 3890 133 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2621 3582 4228 4656 6358 7642 8507 8805 2621
Size: 961 646 428 1702 1284 865 298 3333
Fragments arranged by size:
3333 1702 1284 961 865 646 428 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5943 8180 882
Size: 617 4444 2237 2219
Fragments arranged by size:
4444 2237 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2368 3364 7308 493
Size: 711 1164 996 3944 2702
Fragments arranged by size:
3944 2702 1164 996 711
BsaBI GATnn'nnATC
Cuts at: 2730 4053 4527 5601 9511 2730
Size: 1323 474 1074 3910 2736
Fragments arranged by size:
3910 2736 1323 1074 474
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6333 7324 7706 8341
Size: 53 83 186 936 4800 991 382 635
Cuts at: 8341 275
Size: 1451
Fragments arranged by size:
4800 1451 991 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3115 7950 8348 8622 8769 9180 3115
Size: 4835 398 274 147 411 3452
Fragments arranged by size:
4835 3452 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2244 2274 1178
Size: 30 165 30 841 30 8421
Fragments arranged by size:
8421 841 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2071 4151 5038 6229 6375 1186
Size: 874 11 2080 887 1191 146 4328
Fragments arranged by size:
4328 2080 1191 887 874 146 11
BseYI C'CCAG_C
Cuts at: 3520 5297 6056 6308 6355 8720 9216 3520
Size: 1777 759 252 47 2365 496 3821
Fragments arranged by size:
3821 2365 1777 759 496 252 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2213 4397 5504 1163
Size: 573 477 2184 1107 5176
Fragments arranged by size:
5176 2184 1107 573 477
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6967 7728 8756 1540
Size: 275 186 4966 761 1028 2301
Fragments arranged by size:
4966 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3712 6000 6075 7578 7663 8734 3712
Size: 2288 75 1503 85 1071 4495
Fragments arranged by size:
4495 2288 1503 1071 85 75
BsmI GAATG_Cn'
Cuts at: 2364 2980 3895 3942 5133 5316 7028 7121 2364
Size: 616 915 47 1191 183 1712 93 4760
Fragments arranged by size:
4760 1712 1191 915 616 183 93 47
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3712 5399 5516 5700 6000 6009 6075
Size: 399 1922 1687 117 184 300 9 66
Cuts at: 6075 6836 7578 7663 8734 1391
Size: 761 742 85 1071 2174
Fragments arranged by size:
2174 1922 1687 1071 761 742 399 300
184 117 85 66 9
BspEI T'CCGG_A
Cuts at: 3115 9180 3115
Size: 6065 3452
BspHI T'CATG_A
Cuts at: 4236 4516 7406 9136 4236
Size: 280 2890 1730 4617
Fragments arranged by size:
4617 2890 1730 280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3153 3319 6872 844
Size: 2309 166 3553 3489
Fragments arranged by size:
3553 3489 2309 166
BsrBI CCG'CTC
Cuts at: 1394 1823 7404 1394
Size: 429 5581 3507
Fragments arranged by size:
5581 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5232 6451 6463 8012 8186 65
Size: 1183 3984 1219 12 1549 174 1396
Fragments arranged by size:
3984 1549 1396 1219 1183 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 8161 8348 1700
Size: 110 6351 187 2869
Fragments arranged by size:
6351 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9517
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9517
BssSI C'ACGA_G
Cuts at: 7577 8589 7577
Size: 1012 8505
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4445 794
Size: 3651 5866
BstBI TT'CG_AA
Cuts at: 8321 8321
Size: 9517
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9517
BstXI CCAn_nnnn'nTGG
Cuts at: 2995 3256 5707 6016 2995
Size: 261 2451 309 6496
Fragments arranged by size:
6496 2451 309 261
BstYI r'GATC_y
Cuts at: 1972 2944 3024 4522 4667 5602 5812 6847 7214
Size: 972 80 1498 145 935 210 1035 367
Cuts at: 7214 7599 7616 9068 9154 9166 9512 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1498 1452 1035 972 935 385 367
346 210 145 86 80 17 12
Bsu36I CC'TnA_GG
Cuts at: 4134 4938 5409 6387 4134
Size: 804 471 978 7264
Fragments arranged by size:
7264 978 804 471
BtgI C'CryG_G
Cuts at: 513 1065 2004 4654 5516 6200 513
Size: 552 939 2650 862 684 3830
Fragments arranged by size:
3830 2650 939 862 684 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8778
Fragments arranged by size:
8778 683 56
BtsI GCAGTG_nn'
Cuts at: 2137 3215 5788 7033 7827 7847 2137
Size: 1078 2573 1245 794 20 3807
Fragments arranged by size:
3807 2573 1245 1078 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9517
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4623 4658 4672 4707 6189 6224 529
Size: 35 4059 35 14 35 1482 35 3822
Fragments arranged by size:
4059 3822 1482 35 35 35 35 14
DraI TTT'AAA
Cuts at: 3900 4851 6821 3900
Size: 951 1970 6596
Fragments arranged by size:
6596 1970 951
DraIII CAC_nnn'GTG
Cuts at: 2371 2371
Size: 9517
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3847 8524 809
Size: 507 2531 4677 1802
Fragments arranged by size:
4677 2531 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6408 6964 7853
Size: 54 1425 50 275 18 4578 556 889
Cuts at: 7853 9482 8
Size: 1629 43
Fragments arranged by size:
4578 1629 1425 889 556 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6964 1537
Size: 275 5152 4090
Fragments arranged by size:
5152 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2277 3372 3667 3818 4044 5056 5208 5736
Size: 924 1095 295 151 226 1012 152 528
Cuts at: 5736 6057 7447 1353
Size: 321 1390 3423
Fragments arranged by size:
3423 1390 1095 1012 924 528 321 295
226 152 151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8104 8478 8624 387
Size: 1064 138 6515 374 146 1280
Fragments arranged by size:
6515 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5998 5998
Size: 9517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2520 2583 2842 3049 3432 4580
Size: 393 476 412 63 259 207 383 1148
Cuts at: 4580 5839 6154 7580 8177 8964 9232 1239
Size: 1259 315 1426 597 787 268 1524
Fragments arranged by size:
1524 1426 1259 1148 787 597 476 412
393 383 315 268 259 207 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2785 3074 2785
Size: 289 9228
EcoO109I rG'GnC_Cy
Cuts at: 2106 2792 5095 2106
Size: 686 2303 6528
Fragments arranged by size:
6528 2303 686
EcoRI G'AATT_C
Cuts at: 6564 6826 7204 6564
Size: 262 378 8877
Fragments arranged by size:
8877 378 262
EcoRV GAT'ATC
Cuts at: 2763 2763
Size: 9517
FspI TGC'GCA
Cuts at: 3983 6045 8023 3983
Size: 2062 1978 5477
Fragments arranged by size:
5477 2062 1978
HaeII r_GCGC'y
Cuts at: 1678 2054 6105 8664 1678
Size: 376 4051 2559 2531
Fragments arranged by size:
4051 2559 2531 376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2244 2276 2986 3018 3257
Size: 32 135 32 672 32 710 32 239
Cuts at: 3257 3289 3741 3773 4127 4159 5033 5065 5483
Size: 32 452 32 354 32 874 32 418
Cuts at: 5483 5515 5984 6016 6659 6691 6770 6802 8158
Size: 32 469 32 643 32 79 32 1356
Cuts at: 8158 8190 8232 8264 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1356 874 710 672 643 469 452
418 354 239 135 79 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2839 6861 7107 669
Size: 988 281 901 4022 246 3079
Fragments arranged by size:
4022 3079 988 901 281 246
HindIII A'AGCT_T
Cuts at: 748 4421 748
Size: 3673 5844
HpaI GTT'AAC
Cuts at: 7107 7107
Size: 9517
KpnI G_GTAC'C
Cuts at: 5945 6842 5945
Size: 897 8620
MfeI C'AATT_G
Cuts at: 4635 7116 4635
Size: 2481 7036
MluI A'CGCG_T
Cuts at: 9393 9393
Size: 9517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2230 4944 5054 8631 8815 1305
Size: 925 2714 110 3577 184 2007
Fragments arranged by size:
3577 2714 2007 925 184 110
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6410 10
Size: 54 1425 343 4578 3117
Fragments arranged by size:
4578 3117 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2993 3203 3723 3860 4308 4337 4701
Size: 648 1827 210 520 137 448 29 364
Cuts at: 4701 5141 5664 6657 7475 7834 7993 518
Size: 440 523 993 818 359 159 2042
Fragments arranged by size:
2042 1827 993 818 648 523 520 448
440 364 359 210 159 137 29
MspA1I CmG'CkG
Cuts at: 1606 3435 3661 4861 5715 6162 6639 6933 7610
Size: 1829 226 1200 854 447 477 294 677
Cuts at: 7610 8758 9003 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1829 1200 1148 854 677 477 447
294 245 226
NcoI C'CATG_G
Cuts at: 513 1065 2004 4654 5516 513
Size: 552 939 2650 862 4514
Fragments arranged by size:
4514 2650 939 862 552
NdeI CA'TA_TG
Cuts at: 387 2940 4350 4549 387
Size: 2553 1410 199 5355
Fragments arranged by size:
5355 2553 1410 199
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9517
NotI GC'GGCC_GC
Cuts at: 6964 6964
Size: 9517
NspI r_CATG'y
Cuts at: 1132 1229 4233 4692 5197 6353 6441 6875 8420
Size: 97 3004 459 505 1156 88 434 1545
Cuts at: 8420 1132
Size: 2229
Fragments arranged by size:
3004 2229 1545 1156 505 459 434 97
88
PasI CC'CwG_GG
Cuts at: 1348 5986 1348
Size: 4638 4879
PciI A'CATG_T
Cuts at: 4688 8416 4688
Size: 3728 5789
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2371 2557 3210 3322 1108
Size: 1263 186 653 112 7303
Fragments arranged by size:
7303 1263 653 186 112
PfoI T'CCnGG_A
Cuts at: 2148 2633 4080 2148
Size: 485 1447 7585
Fragments arranged by size:
7585 1447 485
PmeI GTTT'AAAC
Cuts at: 6821 6821
Size: 9517
PmlI CAC'GTG
Cuts at: 2368 2368
Size: 9517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7586 7618 9099 9131 7586
Size: 32 1481 32 7972
Fragments arranged by size:
7972 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2106 2792 5095 2106
Size: 686 2303 6528
Fragments arranged by size:
6528 2303 686
PshAI GACnn'nnGTC
Cuts at: 1613 5715 1613
Size: 4102 5415
PsiI TTA'TAA
Cuts at: 6534 7087 6534
Size: 553 8964
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4092 4124 4092
Size: 32 9485
PstI C_TGCA'G
Cuts at: 830 3313 4600 6869 830
Size: 2483 1287 2269 3478
Fragments arranged by size:
3478 2483 2269 1287
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9517
PvuII CAG'CTG
Cuts at: 3661 4861 5715 6162 6639 3661
Size: 1200 854 447 477 6539
Fragments arranged by size:
6539 1200 854 477 447
SacI G_AGCT'C
Cuts at: 6000 6000
Size: 9517
SalI G'TCGA_C
Cuts at: 1936 6859 1936
Size: 4923 4594
SanDI GG'GwC_CC
Cuts at: 2792 2792
Size: 9517
SapI GCTCTTCn'nnn_
Cuts at: 5736 5736
Size: 9517
SbfI CC_TGCA'GG
Cuts at: 6869 6869
Size: 9517
ScaI AGT'ACT
Cuts at: 6803 7765 9477 6803
Size: 962 1712 6843
Fragments arranged by size:
6843 1712 962
SexAI A'CCwGG_T
Cuts at: 4095 4095
Size: 9517
SfcI C'TryA_G
Cuts at: 826 1045 3309 3372 4596 6451 6865 8000 8681
Size: 219 2264 63 1224 1855 414 1135 681
Cuts at: 8681 8872 826
Size: 191 1471
Fragments arranged by size:
2264 1855 1471 1224 1135 681 414 219
191 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9517
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9517
SmaI CCC'GGG
Cuts at: 1872 6844 1872
Size: 4972 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2600 3597 4243 4671 5849 6337
Size: 197 926 657 997 646 428 1178 488
Cuts at: 6337 7621 8522 8784 820
Size: 1284 901 262 1553
Fragments arranged by size:
1553 1284 1178 997 926 901 657 646
488 428 262 197
SnaBI TAC'GTA
Cuts at: 493 7308 493
Size: 6815 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9517
SphI G_CATG'C
Cuts at: 1132 6875 1132
Size: 5743 3774
SspI AAT'ATT
Cuts at: 5 52 3538 4070 5112 7441 5
Size: 47 3486 532 1042 2329 2081
Fragments arranged by size:
3486 2329 2081 1042 532 47
StuI AGG'CCT
Cuts at: 1517 4604 1517
Size: 3087 6430
StyI C'CwwG_G
Cuts at: 513 1065 2004 2013 4654 5516 6956 513
Size: 552 939 9 2641 862 1440 3074
Fragments arranged by size:
3074 2641 1440 939 862 552 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 3850 5845 6560 7544 7561 7714
Size: 1255 417 1853 1995 715 984 17 153
Cuts at: 7714 7899 8344 325
Size: 185 445 1498
Fragments arranged by size:
1995 1853 1498 1255 984 715 445 417
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4839 4909 5330
Size: 274 80 33 51 1443 2862 70 421
Cuts at: 5330 5763 6801 7763 9475 96
Size: 433 1038 962 1712 138
Fragments arranged by size:
2862 1712 1443 1038 962 433 421 274
138 80 70 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5488 5520 9477 9509 5488
Size: 32 3957 32 5496
Fragments arranged by size:
5496 3957 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9517
XbaI T'CTAG_A
Cuts at: 6853 6853
Size: 9517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2372 2558 3699 6612 2372
Size: 186 1141 2913 5277
Fragments arranged by size:
5277 2913 1141 186
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9517
XmaI C'CCGG_G
Cuts at: 1870 6842 1870
Size: 4972 4545
XmnI GAAnn'nnTTC
Cuts at: 2405 3795 5165 7646 2405
Size: 1390 1370 2481 4276
Fragments arranged by size:
4276 2481 1390 1370
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7325 276
Size: 53 83 186 936 5791 2468
Fragments arranged by size:
5791 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacII
SfiI SfoI SrfI SwaI