Restriction map for
pFN21AE3476
(Circular) (Six-base) MAPSORT of: pFN21AE3476.seq Check: 3034 from: 1 to: 9358
pFN21AE3476 9358 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2895 3183 3694 5170 5501 5686 2895
Size: 288 511 1476 331 185 6567
Fragments arranged by size:
6567 1476 511 331 288 185
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7168 278
Size: 53 83 186 936 5632 2468
Fragments arranged by size:
5632 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6701 1937
Size: 4764 4594
Acc65I G'GTAC_C
Cuts at: 6679 6679
Size: 9358
AclI AA'CG_TT
Cuts at: 1563 7485 7858 1563
Size: 5922 373 3063
Fragments arranged by size:
5922 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2348 3557 6305 7421 8805 9073 1632
Size: 716 1209 2748 1116 1384 268 1917
Fragments arranged by size:
2748 1917 1384 1209 1116 716 268
AfeI AGC'GCT
Cuts at: 4248 4248
Size: 9358
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9161
AflIII A'CryG_T
Cuts at: 1203 6346 8257 9234 1203
Size: 5143 1911 977 1327
Fragments arranged by size:
5143 1911 1327 977
AgeI A'CCGG_T
Cuts at: 8189 8189
Size: 9358
AhdI GACnn_n'nnGTC
Cuts at: 2782 4677 8087 2782
Size: 1895 3410 4053
Fragments arranged by size:
4053 3410 1895
AleI CACnn'nnGTG
Cuts at: 1166 2264 5582 1166
Size: 1098 3318 4942
Fragments arranged by size:
4942 3318 1098
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2310 5153 5600 5694 6296 1184
Size: 1126 2843 447 94 602 4246
Fragments arranged by size:
4246 2843 1126 602 447 94
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5003 5035 5003
Size: 32 9326
AlwNI CAG_nnn'CTG
Cuts at: 2155 2950 3268 3370 3779 4000 4105 4279 4363
Size: 795 318 102 409 221 105 174 84
Cuts at: 4363 4723 4847 5185 5203 5410 5548 5695 7101
Size: 360 124 338 18 207 138 147 1406
Cuts at: 7101 8673 2155
Size: 1572 2840
Fragments arranged by size:
2840 1572 1406 795 409 360 338 318
221 207 174 147 138 124 105 102
84 18
ApaI G_GGCC'C
Cuts at: 3081 3432 3550 3631 4405 4987 5633 5639 5646
Size: 351 118 81 774 582 646 6 7
Cuts at: 5646 6356 3081
Size: 710 6083
Fragments arranged by size:
6083 774 710 646 582 351 118 81
7 6
ApaLI G'TGCA_C
Cuts at: 5128 7415 8571 5128
Size: 2287 1156 5915
Fragments arranged by size:
5915 2287 1156
ApoI r'AATT_y
Cuts at: 1493 6667 6893 7045 9154 1493
Size: 5174 226 152 2109 1697
Fragments arranged by size:
5174 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7912 160
Size: 872 6880 1606
Fragments arranged by size:
6880 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9358
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2510 3624 3654 4404 5525 5532
Size: 73 119 448 1114 30 750 1121 7
Cuts at: 5532 5632 6683 1870
Size: 100 1051 4545
Fragments arranged by size:
4545 1121 1114 1051 750 448 119 100
73 30 7
BamHI G'GATC_C
Cuts at: 6688 7055 6688
Size: 367 8991
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4151 4870 5330 5513 5674
Size: 325 217 627 2364 719 460 183 161
Cuts at: 5674 5726 5797 5846 6110 6198 6374 6468 6679
Size: 52 71 49 264 88 176 94 211
Cuts at: 6679 8134 618
Size: 1455 1842
Fragments arranged by size:
2364 1842 1455 719 627 460 325 264
217 211 183 176 161 94 88 71
52 49
BanII G_rGCy'C
Cuts at: 1391 2070 2112 3081 3432 3540 3550 3631 3638
Size: 679 42 969 351 108 10 81 7
Cuts at: 3638 4377 4405 4466 4987 5346 5423 5633 5639
Size: 739 28 61 521 359 77 210 6
Cuts at: 5639 5646 6095 6356 6584 6653 6677 1391
Size: 7 449 261 228 69 24 4072
Fragments arranged by size:
4072 969 739 679 521 449 359 351
261 228 210 108 81 77 69 61
42 28 24 10 7 7 6
BbeI G_GCGC'C
Cuts at: 5730 6202 6378 6472 5730
Size: 472 176 94 8616
Fragments arranged by size:
8616 472 176 94
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4610 928
Size: 971 2711 5676
Fragments arranged by size:
5676 2711 971
BbvCI CC'TCA_GC
Cuts at: 3395 4058 5864 3395
Size: 663 1806 6889
Fragments arranged by size:
6889 1806 663
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7549 7583 1903
Size: 34 5612 34 3678
Fragments arranged by size:
5612 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7250 8460 7250
Size: 1210 8148
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9358
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2763 4719 4772
Size: 107 122 71 631 605 1091 1956 53
Cuts at: 4772 7969 136
Size: 3197 1525
Fragments arranged by size:
3197 1956 1525 1091 631 605 122 107
71 53
BglII A'GATC_T
Cuts at: 2083 2220 4531 5002 9353 2083
Size: 137 2311 471 4351 2088
Fragments arranged by size:
4351 2311 2088 471 137
BlpI GC'TnA_GC
Cuts at: 2111 2483 3290 3938 4323 4626 4967 5858 6578
Size: 372 807 648 385 303 341 891 720
Cuts at: 6578 6775 2111
Size: 197 4694
Fragments arranged by size:
4694 891 807 720 648 385 372 341
303 197
Bme1580I G_kGCm'C
Cuts at: 1790 2628 3081 3432 3550 3631 4405 4768 4794
Size: 838 453 351 118 81 774 363 26
Cuts at: 4794 4987 5132 5633 5639 5646 6356 7419 8575
Size: 193 145 501 6 7 710 1063 1156
Cuts at: 8575 1790
Size: 2573
Fragments arranged by size:
2573 1156 1063 838 774 710 501 453
363 351 193 145 118 81 26 7
6
BmgBI CAC'GTC
Cuts at: 1318 2905 2914 6028 1318
Size: 1587 9 3114 4648
Fragments arranged by size:
4648 3114 1587 9
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2292 2515 3037
Size: 416 53 83 399 24 875 223 522
Cuts at: 3037 4042 4971 5011 5418 5852 7089 8047 442
Size: 1005 929 40 407 434 1237 958 1753
Fragments arranged by size:
1753 1237 1005 958 929 875 522 434
416 407 399 223 83 53 40 24
BmtI G_CTAG'C
Cuts at: 1056 3618 1056
Size: 2562 6796
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3244 4099 3244
Size: 855 8503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 3245 3269 3290 3362 3665 3695 3884
Size: 809 1197 24 21 72 303 30 189
Cuts at: 3884 4022 4151 4253 4271 5513 8018 1239
Size: 138 129 102 18 1242 2505 2579
Fragments arranged by size:
2579 2505 1242 1197 809 303 189 138
129 102 72 30 24 21 18
Bpu10I CC'TnA_GC
Cuts at: 2156 3066 3395 3525 3632 3695 4058 5864 2156
Size: 910 329 130 107 63 363 1806 5650
Fragments arranged by size:
5650 1806 910 363 329 130 107 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3761 4069 4159 5231 5437 6047 7483 8348 8646
Size: 308 90 1072 206 610 1436 865 298
Cuts at: 8646 3761
Size: 4473
Fragments arranged by size:
4473 1436 1072 865 610 308 298 206
90
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3776 4087 6610 8021 882
Size: 617 2277 311 2523 1411 2219
Fragments arranged by size:
2523 2277 2219 1411 617 311
BsaAI yAC'GTr
Cuts at: 493 1204 5127 5905 6349 7149 493
Size: 711 3923 778 444 800 2702
Fragments arranged by size:
3923 2702 800 778 711 444
BsaBI GATnn'nnATC
Cuts at: 9352 9352
Size: 9358
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2705 2825 4950 5727
Size: 53 83 186 936 1172 120 2125 777
Cuts at: 5727 6199 6375 6469 7165 7547 8182 275
Size: 472 176 94 696 382 635 1451
Fragments arranged by size:
2125 1451 1172 936 777 696 635 472
382 186 176 120 94 83 53
BsaWI w'CCGG_w
Cuts at: 2997 3930 4458 7791 8189 8463 8610 9021 2997
Size: 933 528 3333 398 274 147 411 3334
Fragments arranged by size:
3334 3333 933 528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2420 2450 2633 2663 2702
Size: 30 165 30 1017 30 183 30 39
Cuts at: 2702 2732 3893 3923 4118 4148 4226 4256 6437
Size: 30 1161 30 195 30 78 30 2181
Cuts at: 6437 6467 1178
Size: 30 4069
Fragments arranged by size:
4069 2181 1161 1017 195 183 165 78
39 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2256 2372 2441 2759 3578 3758 3914 4139
Size: 1070 116 69 318 819 180 156 225
Cuts at: 4139 4406 4536 4793 5351 5819 6046 6266 1186
Size: 267 130 257 558 468 227 220 4278
Fragments arranged by size:
4278 1070 819 558 468 318 267 257
227 225 220 180 156 130 116 69
BseYI C'CCAG_C
Cuts at: 2160 2569 3433 3639 4041 4062 4311 4702 5103
Size: 409 864 206 402 21 249 391 401
Cuts at: 5103 5185 5462 5626 5704 5782 5967 6472 8561
Size: 82 277 164 78 78 185 505 2089
Cuts at: 8561 9057 2160
Size: 496 2461
Fragments arranged by size:
2461 2089 864 505 496 409 402 401
391 277 249 206 185 164 82 78
78 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2084 2294 2390 2546 3299 3383 4322
Size: 573 348 210 96 156 753 84 939
Cuts at: 4322 4672 5423 5448 5708 6068 1163
Size: 350 751 25 260 360 4453
Fragments arranged by size:
4453 939 753 751 573 360 350 348
260 210 156 96 84 25
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2239 5713 6808 7569 8597 1540
Size: 275 186 238 3474 1095 761 1028 2301
Fragments arranged by size:
3474 2301 1095 1028 761 275 238 186
BsiHKAI G_wGCw'C
Cuts at: 2112 2373 3540 3638 4377 4466 4588 5132 5589
Size: 261 1167 98 739 89 122 544 457
Cuts at: 5589 5616 6653 7419 7504 8575 2112
Size: 27 1037 766 85 1071 2895
Fragments arranged by size:
2895 1167 1071 1037 766 739 544 457
261 122 98 89 85 27
BsmI GAATG_Cn'
Cuts at: 6869 6962 6869
Size: 93 9265
BsmBI CGTCTCn'nnnn_
Cuts at: 2507 2666 2816 4013 5477 6498 2507
Size: 159 150 1197 1464 1021 5367
Fragments arranged by size:
5367 1464 1197 1021 159 150
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2112 2373 2628 3081 3432 3540
Size: 399 280 42 261 255 453 351 108
Cuts at: 3540 3550 3631 3638 4377 4405 4466 4588 4768
Size: 10 81 7 739 28 61 122 180
Cuts at: 4768 4794 4987 5132 5346 5423 5589 5616 5633
Size: 26 193 145 214 77 166 27 17
Cuts at: 5633 5639 5646 6095 6356 6584 6653 6677 7419
Size: 6 7 449 261 228 69 24 742
Cuts at: 7419 7504 8575 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 739 453 449 399 351
280 261 261 255 228 214 193 180
166 145 122 108 85 81 77 69
61 42 28 27 26 24 17 10
7 7 6
BspEI T'CCGG_A
Cuts at: 2997 4458 9021 2997
Size: 1461 4563 3334
Fragments arranged by size:
4563 3334 1461
BspHI T'CATG_A
Cuts at: 2868 7247 8977 2868
Size: 4379 1730 3249
Fragments arranged by size:
4379 3249 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2895 3183 3694 4104 4984 5082 5170 5501
Size: 2051 288 511 410 880 98 88 331
Cuts at: 5501 5686 6592 6713 844
Size: 185 906 121 3489
Fragments arranged by size:
3489 2051 906 880 511 410 331 288
185 121 98 88
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 2362 2968 3034 3253 3955 4012
Size: 429 188 351 606 66 219 702 57
Cuts at: 4012 4288 5164 6061 7245 1394
Size: 276 876 897 1184 3507
Fragments arranged by size:
3507 1184 897 876 702 606 429 351
276 219 188 66 57
BsrDI GCAATG_nn'
Cuts at: 65 1248 7853 8027 65
Size: 1183 6605 174 1396
Fragments arranged by size:
6605 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 3699 5394 5712 5732 5789 6195
Size: 110 241 1648 1695 318 20 57 406
Cuts at: 6195 8002 8189 1700
Size: 1807 187 2869
Fragments arranged by size:
2869 1807 1695 1648 406 318 241 187
110 57 20
BsrGI T'GTAC_A
Cuts at: 96 5238 96
Size: 5142 4216
BssHII G'CGCG_C
Cuts at: 1925 2020 2022 1925
Size: 95 2 9261
Fragments arranged by size:
9261 95 2
BssSI C'ACGA_G
Cuts at: 2105 2372 2876 5928 5936 7418 8430 2105
Size: 267 504 3052 8 1482 1012 3033
Fragments arranged by size:
3052 3033 1482 1012 504 267 8
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3370 4279 4592 5203 5846 6055 794
Size: 2576 909 313 611 643 209 4097
Fragments arranged by size:
4097 2576 909 643 611 313 209
BstBI TT'CG_AA
Cuts at: 3015 8162 3015
Size: 5147 4211
BstEII G'GTnAC_C
Cuts at: 1184 2257 2395 1184
Size: 1073 138 8147
Fragments arranged by size:
8147 1073 138
BstXI CCAn_nnnn'nTGG
Cuts at: 2677 3425 3793 4117 2677
Size: 748 368 324 7918
Fragments arranged by size:
7918 748 368 324
BstYI r'GATC_y
Cuts at: 1972 2083 2220 2727 4260 4531 5002 5520 6688
Size: 111 137 507 1533 271 471 518 1168
Cuts at: 6688 7055 7440 7457 8909 8995 9007 9353 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1533 1452 1168 518 507 471 385
367 346 271 137 111 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3202 3804 3816 4882 4961 5802 6137 3202
Size: 602 12 1066 79 841 335 6423
Fragments arranged by size:
6423 1066 841 602 335 79 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2262 2739 2977 3081 3151 4874
Size: 552 939 258 477 238 104 70 1723
Cuts at: 4874 5455 5821 5850 5961 6421 6480 513
Size: 581 366 29 111 460 59 3391
Fragments arranged by size:
3391 1723 939 581 552 477 460 366
258 238 111 104 70 59 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8619
Fragments arranged by size:
8619 683 56
BtsI GCAGTG_nn'
Cuts at: 4590 4845 5118 5889 6874 7668 7688 4590
Size: 255 273 771 985 794 20 6260
Fragments arranged by size:
6260 985 794 771 273 255 20
ClaI AT'CG_AT
Cuts at: 1554 6015 1554
Size: 4461 4897
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5911 5946 529
Size: 35 5347 35 3941
Fragments arranged by size:
5347 3941 35 35
DraI TTT'AAA
Cuts at: 6662 6662
Size: 9358
DraIII CAC_nnn'GTG
Cuts at: 2774 5127 5846 2774
Size: 2353 719 6286
Fragments arranged by size:
6286 2353 719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6541 8365 809
Size: 507 5225 1824 1802
Fragments arranged by size:
5225 1824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2236 2547 3166
Size: 54 1425 50 275 18 406 311 619
Cuts at: 3166 3498 3606 3843 4107 4172 4224 5219 5452
Size: 332 108 237 264 65 52 995 233
Cuts at: 5452 5932 5948 6805 7694 9323 8
Size: 480 16 857 889 1629 43
Fragments arranged by size:
1629 1425 995 889 857 619 480 406
332 311 275 264 237 233 108 65
54 52 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2236 6805 1537
Size: 275 424 4569 4090
Fragments arranged by size:
4569 4090 424 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2324 2958 5888 6396 6515 7288 1353
Size: 971 634 2930 508 119 773 3423
Fragments arranged by size:
3423 2930 971 773 634 508 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2930 5501 6113 7945 8319 8465
Size: 1064 138 1341 2571 612 1832 374 146
Cuts at: 8465 387
Size: 1280
Fragments arranged by size:
2571 1832 1341 1280 1064 612 374 146
138
EcoICRI GAG'CTC
Cuts at: 2110 3538 3636 4375 4464 6651 2110
Size: 1428 98 739 89 2187 4817
Fragments arranged by size:
4817 2187 1428 739 98 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2348 3245 3269 3290 3362 3557
Size: 393 416 300 897 24 21 72 195
Cuts at: 3557 3665 3695 3884 4022 4151 4253 4271 5513
Size: 108 30 189 138 129 102 18 1242
Cuts at: 5513 6305 7421 8018 8805 9073 1239
Size: 792 1116 597 787 268 1524
Fragments arranged by size:
1524 1242 1116 897 792 787 597 416
393 300 268 195 189 138 129 108
102 72 30 24 21 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3467 4225 4367 4991 5309 5834 6136 6599 3467
Size: 758 142 624 318 525 302 463 6226
Fragments arranged by size:
6226 758 624 525 463 318 302 142
EcoO109I rG'GnC_Cy
Cuts at: 2467 3077 3132 3156 3310 3429 3546 3628 3797
Size: 610 55 24 154 119 117 82 169
Cuts at: 3797 4102 4276 4360 4486 4720 4774 4983 4984
Size: 305 174 84 126 234 54 209 1
Cuts at: 4984 4996 5011 5071 5636 5643 5827 6289 6353
Size: 12 15 60 565 7 184 462 64
Cuts at: 6353 2467
Size: 5472
Fragments arranged by size:
5472 610 565 462 305 234 209 184
174 169 154 126 119 117 84 82
64 60 55 54 24 15 12 7
1
EcoRI G'AATT_C
Cuts at: 6667 7045 6667
Size: 378 8980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6247 6247
Size: 9358
FspI TGC'GCA
Cuts at: 4562 5363 7864 4562
Size: 801 2501 6056
Fragments arranged by size:
6056 2501 801
HaeII r_GCGC'y
Cuts at: 1678 2099 2928 4250 4330 5608 5656 5730 5783
Size: 421 829 1322 80 1278 48 74 53
Cuts at: 5783 5960 6202 6229 6378 6472 8505 1678
Size: 177 242 27 149 94 2033 2531
Fragments arranged by size:
2531 2033 1322 1278 829 421 242 177
149 94 80 74 53 48 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2633 2665 2702 2734 2768
Size: 32 135 32 1061 32 37 32 34
Cuts at: 2768 2800 3392 3424 3734 3766 4118 4150 4226
Size: 32 592 32 310 32 352 32 76
Cuts at: 4226 4258 4663 4695 6542 6574 7999 8031 8073
Size: 32 405 32 1847 32 1425 32 42
Cuts at: 8073 8105 1373
Size: 32 2626
Fragments arranged by size:
2626 1847 1425 1061 592 405 352 310
135 76 42 37 34 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6702 6948 669
Size: 988 281 4764 246 3079
Fragments arranged by size:
4764 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9358
HpaI GTT'AAC
Cuts at: 6948 6948
Size: 9358
KasI G'GCGC_C
Cuts at: 5726 6198 6374 6468 5726
Size: 472 176 94 8616
Fragments arranged by size:
8616 472 176 94
KpnI G_GTAC'C
Cuts at: 6683 6683
Size: 9358
MfeI C'AATT_G
Cuts at: 6957 6957
Size: 9358
MluI A'CGCG_T
Cuts at: 9234 9234
Size: 9358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3117 6228 8472 8656 1305
Size: 1812 3111 2244 184 2007
Fragments arranged by size:
3111 2244 2007 1812 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2549 4109 5934 5950 10
Size: 54 1425 343 717 1560 1825 16 3418
Fragments arranged by size:
3418 1825 1560 1425 717 343 54 16
MslI CAynn'nnrTG
Cuts at: 518 1166 2264 2303 2586 4115 5582 5903 6017
Size: 648 1098 39 283 1529 1467 321 114
Cuts at: 6017 7316 7675 7834 518
Size: 1299 359 159 2042
Fragments arranged by size:
2042 1529 1467 1299 1098 648 359 321
283 159 114 39
MspA1I CmG'CkG
Cuts at: 1606 2035 2118 2569 2947 3437 3643 3715 3862
Size: 429 83 451 378 490 206 72 147
Cuts at: 3862 3916 3997 4062 4315 4686 4876 5107 5200
Size: 54 81 65 253 371 190 231 93
Cuts at: 5200 5275 5410 5457 5555 5626 5755 5823 6148
Size: 75 135 47 98 71 129 68 325
Cuts at: 6148 6205 6423 6439 6774 7451 8599 8844 1606
Size: 57 218 16 335 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 490 451 429 378 371
335 325 253 245 231 218 206 190
147 135 129 98 93 83 81 75
72 71 68 65 57 54 47 16
NaeI GCC'GGC
Cuts at: 2053 3701 5396 6197 2053
Size: 1648 1695 801 5214
Fragments arranged by size:
5214 1695 1648 801
NarI GG'CG_CC
Cuts at: 5727 6199 6375 6469 5727
Size: 472 176 94 8616
Fragments arranged by size:
8616 472 176 94
NcoI C'CATG_G
Cuts at: 513 1065 2004 2739 3081 5961 6480 513
Size: 552 939 735 342 2880 519 3391
Fragments arranged by size:
3391 2880 939 735 552 519 342
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9358
NgoMIV G'CCGG_C
Cuts at: 2051 3699 5394 6195 2051
Size: 1648 1695 801 5214
Fragments arranged by size:
5214 1695 1648 801
NheI G'CTAG_C
Cuts at: 1052 3614 1052
Size: 2562 6796
NotI GC'GGCC_GC
Cuts at: 6805 6805
Size: 9358
NspI r_CATG'y
Cuts at: 1132 1229 6716 8261 1132
Size: 97 5487 1545 2229
Fragments arranged by size:
5487 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3550 4816 5639 6568 1348
Size: 2202 1266 823 929 4138
Fragments arranged by size:
4138 2202 1266 929 823
PciI A'CATG_T
Cuts at: 8257 8257
Size: 9358
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3268 4723 5548 5967 1108
Size: 2160 1455 825 419 4499
Fragments arranged by size:
4499 2160 1455 825 419
PfoI T'CCnGG_A
Cuts at: 2352 6174 2352
Size: 3822 5536
PmeI GTTT'AAAC
Cuts at: 6662 6662
Size: 9358
PmlI CAC'GTG
Cuts at: 5127 6349 5127
Size: 1222 8136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7427 7459 8940 8972 7427
Size: 32 1481 32 7813
Fragments arranged by size:
7813 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2467 3132 3797 4486 4996 5011 5071 6289 2467
Size: 665 665 689 510 15 60 1218 5536
Fragments arranged by size:
5536 1218 689 665 665 510 60 15
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9358
PsiI TTA'TAA
Cuts at: 6928 6928
Size: 9358
PspOMI G'GGCC_C
Cuts at: 3077 3428 3546 3627 4401 4983 5629 5635 5642
Size: 351 118 81 774 582 646 6 7
Cuts at: 5642 6352 3077
Size: 710 6083
Fragments arranged by size:
6083 774 710 646 582 351 118 81
7 6
PstI C_TGCA'G
Cuts at: 830 2205 2736 2892 3522 3545 3720 3921 4101
Size: 1375 531 156 630 23 175 201 180
Cuts at: 4101 4209 4242 4284 4320 4338 4995 5181 5199
Size: 108 33 42 36 18 657 186 18
Cuts at: 5199 5571 5673 6710 830
Size: 372 102 1037 3478
Fragments arranged by size:
3478 1375 1037 657 630 531 372 201
186 180 175 156 108 102 42 36
33 23 18 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9358
PvuII CAG'CTG
Cuts at: 2035 2569 2947 3437 3643 3715 3862 3916 4062
Size: 534 378 490 206 72 147 54 146
Cuts at: 4062 4315 5107 5410 5555 5626 6148 6205 2035
Size: 253 792 303 145 71 522 57 5188
Fragments arranged by size:
5188 792 534 522 490 378 303 253
206 147 146 145 72 71 57 54
SacI G_AGCT'C
Cuts at: 2112 3540 3638 4377 4466 6653 2112
Size: 1428 98 739 89 2187 4817
Fragments arranged by size:
4817 2187 1428 739 98 89
SacII CC_GC'GG
Cuts at: 4877 5458 5824 6424 4877
Size: 581 366 600 7811
Fragments arranged by size:
7811 600 581 366
SalI G'TCGA_C
Cuts at: 1936 6700 1936
Size: 4764 4594
SanDI GG'GwC_CC
Cuts at: 3132 3797 5011 3132
Size: 665 1214 7479
Fragments arranged by size:
7479 1214 665
SapI GCTCTTCn'nnn_
Cuts at: 2958 2958
Size: 9358
SbfI CC_TGCA'GG
Cuts at: 2736 4101 4284 4995 5571 6710 2736
Size: 1365 183 711 576 1139 5384
Fragments arranged by size:
5384 1365 1139 711 576 183
ScaI AGT'ACT
Cuts at: 7606 9318 7606
Size: 1712 7646
SfcI C'TryA_G
Cuts at: 826 1045 2201 2732 2888 3518 3541 3581 3716
Size: 219 1156 531 156 630 23 40 135
Cuts at: 3716 3917 4097 4205 4238 4280 4316 4334 4608
Size: 201 180 108 33 42 36 18 274
Cuts at: 4608 4991 5177 5195 5567 5669 6706 7841 8522
Size: 383 186 18 372 102 1037 1135 681
Cuts at: 8522 8713 826
Size: 191 1471
Fragments arranged by size:
1471 1156 1135 1037 681 630 531 383
372 274 219 201 191 186 180 156
135 108 102 42 40 36 33 23
18 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 2763 4719 2763
Size: 1956 7402
SfoI GGC'GCC
Cuts at: 5728 6200 6376 6470 5728
Size: 472 176 94 8616
Fragments arranged by size:
8616 472 176 94
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9358
SgrAI Cr'CCGG_yG
Cuts at: 1700 5732 1700
Size: 4032 5326
SmaI CCC'GGG
Cuts at: 1872 2064 3626 3656 5634 6685 1872
Size: 192 1562 30 1978 1051 4545
Fragments arranged by size:
4545 1978 1562 1051 192 30
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3740 4084 4138 5246 5416 5532
Size: 197 926 1797 344 54 1108 170 116
Cuts at: 5532 6062 7462 8363 8625 820
Size: 530 1400 901 262 1553
Fragments arranged by size:
1797 1553 1400 1108 926 901 530 344
262 197 170 116 54
SnaBI TAC'GTA
Cuts at: 493 7149 493
Size: 6656 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9358
SphI G_CATG'C
Cuts at: 1132 6716 1132
Size: 5584 3774
SrfI GCCC'GGGC
Cuts at: 3656 5634 3656
Size: 1978 7380
SspI AAT'ATT
Cuts at: 5 52 7282 5
Size: 47 7230 2081
Fragments arranged by size:
7230 2081 47
StuI AGG'CCT
Cuts at: 1517 3407 4220 5433 6495 1517
Size: 1890 813 1213 1062 4380
Fragments arranged by size:
4380 1890 1213 1062 813
StyI C'CwwG_G
Cuts at: 513 1065 2004 2739 2844 3081 4482 5961 6480
Size: 552 939 735 105 237 1401 1479 519
Cuts at: 6480 6797 513
Size: 317 3074
Fragments arranged by size:
3074 1479 1401 939 735 552 519 317
237 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2383 3495 4054 4169 4603 5177 5822
Size: 1255 803 1112 559 115 434 574 645
Cuts at: 5822 5834 6034 7385 7402 7555 7740 8185 325
Size: 12 200 1351 17 153 185 445 1498
Fragments arranged by size:
1498 1351 1255 1112 803 645 574 559
445 434 200 185 153 115 17 12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4822 5238 6343
Size: 274 80 33 51 1443 2845 416 1105
Cuts at: 6343 7604 9316 96
Size: 1261 1712 138
Fragments arranged by size:
2845 1712 1443 1261 1105 416 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3893 3925 4172 4204 6437 6469 9318 9350 3893
Size: 32 247 32 2233 32 2849 32 3901
Fragments arranged by size:
3901 2849 2233 247 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2603 1316
Size: 1287 8071
XbaI T'CTAG_A
Cuts at: 6694 6694
Size: 9358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2677 2775 3100 3887 2677
Size: 98 325 787 8148
Fragments arranged by size:
8148 787 325 98
XhoI C'TCGA_G
Cuts at: 1943 5532 1943
Size: 3589 5769
XmaI C'CCGG_G
Cuts at: 1870 2062 3624 3654 5632 6683 1870
Size: 192 1562 30 1978 1051 4545
Fragments arranged by size:
4545 1978 1562 1051 192 30
XmnI GAAnn'nnTTC
Cuts at: 4617 7487 4617
Size: 2870 6488
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7166 276
Size: 53 83 186 936 5632 2468
Fragments arranged by size:
5632 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI
FspAI NruI NsiI PacI PspXI PsrI RsrII SexAI
SwaI