Restriction map for
pFN21AE3472
(Circular) (Six-base) MAPSORT of: pFN21AE3472.seq Check: 3466 from: 1 to: 9241
pFN21AE3472 9241 bp
With 182 enzymes: SgfI *
July 13, 2017 17:10 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7051 278
Size: 53 83 186 936 5515 2468
Fragments arranged by size:
5515 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3945 4056 6584 1937
Size: 2008 111 2528 4594
Fragments arranged by size:
4594 2528 2008 111
Acc65I G'GTAC_C
Cuts at: 6562 6562
Size: 9241
AclI AA'CG_TT
Cuts at: 1563 7368 7741 1563
Size: 5805 373 3063
Fragments arranged by size:
5805 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2591 3099 4016 5006 5574 6533 7304 8688
Size: 959 508 917 990 568 959 771 1384
Cuts at: 8688 8956 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 990 959 959 917 771 568
508 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9044
AflIII A'CryG_T
Cuts at: 1203 5942 8140 9117 1203
Size: 4739 2198 977 1327
Fragments arranged by size:
4739 2198 1327 977
AgeI A'CCGG_T
Cuts at: 8072 8072
Size: 9241
AhdI GACnn_n'nnGTC
Cuts at: 7970 7970
Size: 9241
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9241
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5252 5284 6048 6080 5252
Size: 32 764 32 8413
Fragments arranged by size:
8413 764 32 32
AlwNI CAG_nnn'CTG
Cuts at: 5100 6984 8556 5100
Size: 1884 1572 5785
Fragments arranged by size:
5785 1884 1572
ApaI G_GGCC'C
Cuts at: 3787 3787
Size: 9241
ApaLI G'TGCA_C
Cuts at: 3051 4714 7298 8454 3051
Size: 1663 2584 1156 3838
Fragments arranged by size:
3838 2584 1663 1156
ApoI r'AATT_y
Cuts at: 1493 3148 3253 3817 4304 4316 6550 6776 6928
Size: 1655 105 564 487 12 2234 226 152
Cuts at: 6928 9037 1493
Size: 2109 1697
Fragments arranged by size:
2234 2109 1697 1655 564 487 226 152
105 12
AseI AT'TA_AT
Cuts at: 160 1032 4937 7795 160
Size: 872 3905 2858 1606
Fragments arranged by size:
3905 2858 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9241
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2410 2432 3844 6566 1870
Size: 73 104 363 22 1412 2722 4545
Fragments arranged by size:
4545 2722 1412 363 104 73 22
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4476 4509 5427 5460 4476
Size: 33 918 33 8257
Fragments arranged by size:
8257 918 33 33
BamHI G'GATC_C
Cuts at: 4184 6571 6938 4184
Size: 2387 367 6487
Fragments arranged by size:
6487 2387 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2332 2366 2392 2405
Size: 325 217 627 264 281 34 26 13
Cuts at: 2405 2446 6119 6253 6562 8017 618
Size: 41 3673 134 309 1455 1842
Fragments arranged by size:
3673 1842 1455 627 325 309 281 264
217 134 41 34 26 13
BanII G_rGCy'C
Cuts at: 1391 2069 2095 2289 2433 2875 3787 5388 6560
Size: 678 26 194 144 442 912 1601 1172
Cuts at: 6560 1391
Size: 4072
Fragments arranged by size:
4072 1601 1172 912 678 442 194 144
26
BbeI G_GCGC'C
Cuts at: 2055 2370 2396 2409 2450 2055
Size: 315 26 13 41 8846
Fragments arranged by size:
8846 315 41 26 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3390 3861 4632 5727 928
Size: 971 1491 471 771 1095 4442
Fragments arranged by size:
4442 1491 1095 971 771 471
BbvCI CC'TCA_GC
Cuts at: 5622 5622
Size: 9241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5973 6007 7432 7466 1903
Size: 34 4036 34 1425 34 3678
Fragments arranged by size:
4036 3678 1425 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5662 7133 8343 5662
Size: 1471 1210 6560
Fragments arranged by size:
6560 1471 1210
BclI T'GATC_A
Cuts at: 1548 2556 1548
Size: 1008 8233
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2099 2364
Size: 107 122 71 631 605 337 90 265
Cuts at: 2364 6188 7852 136
Size: 3824 1664 1525
Fragments arranged by size:
3824 1664 1525 631 605 337 265 122
107 90 71
BglII A'GATC_T
Cuts at: 3479 3491 9236 3479
Size: 12 5745 3484
Fragments arranged by size:
5745 3484 12
BlpI GC'TnA_GC
Cuts at: 3675 4356 6658 3675
Size: 681 2302 6258
Fragments arranged by size:
6258 2302 681
Bme1580I G_kGCm'C
Cuts at: 1790 3055 3787 4718 7302 8458 1790
Size: 1265 732 931 2584 1156 2573
Fragments arranged by size:
2584 2573 1265 1156 931 732
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2648 2825 5129
Size: 416 53 83 399 24 1231 177 2304
Cuts at: 5129 5319 5342 6242 6972 7930 442
Size: 190 23 900 730 958 1753
Fragments arranged by size:
2304 1753 1231 958 900 730 416 399
190 177 83 53 24 23
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2111 5407 2111
Size: 3296 5945
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2685 2787 4028 5149 5377 7901 1239
Size: 1446 102 1241 1121 228 2524 2579
Fragments arranged by size:
2579 2524 1446 1241 1121 228 102
Bpu10I CC'TnA_GC
Cuts at: 2226 5622 2226
Size: 3396 5845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2081 3181 4486 5769 7366 8231 8529 2081
Size: 1100 1305 1283 1597 865 298 2793
Fragments arranged by size:
2793 1597 1305 1283 1100 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7904 882
Size: 617 6405 2219
Fragments arranged by size:
6405 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3056 5943 7032 493
Size: 711 1852 2887 1089 2702
Fragments arranged by size:
2887 2702 1852 1089 711
BsaBI GATnn'nnATC
Cuts at: 5517 9235 5517
Size: 3718 5523
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 2367 2393 2406
Size: 53 83 186 936 519 315 26 13
Cuts at: 2406 2447 3760 7048 7430 8065 275
Size: 41 1313 3288 382 635 1451
Fragments arranged by size:
3288 1451 1313 936 635 519 382 315
186 83 53 41 26 13
BsaWI w'CCGG_w
Cuts at: 7674 8072 8346 8493 8904 7674
Size: 398 274 147 411 8011
Fragments arranged by size:
8011 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3882 3912 6048 6078 6397
Size: 30 165 30 2479 30 2136 30 319
Cuts at: 6397 6427 1178
Size: 30 3992
Fragments arranged by size:
3992 2479 2136 319 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2103 2254 3009 4900 5049 5369 1186
Size: 917 151 755 1891 149 320 5058
Fragments arranged by size:
5058 1891 917 755 320 151 149
BseYI C'CCAG_C
Cuts at: 8444 8940 8444
Size: 496 8745
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3389 3540 4098 6228 1163
Size: 573 1653 151 558 2130 4176
Fragments arranged by size:
4176 2130 1653 573 558 151
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2427 2488 6691 7452 8480 1540
Size: 275 186 426 61 4203 761 1028 2301
Fragments arranged by size:
4203 2301 1028 761 426 275 186 61
BsiHKAI G_wGCw'C
Cuts at: 3055 3812 4718 5388 7302 7387 8458 3055
Size: 757 906 670 1914 85 1071 3838
Fragments arranged by size:
3838 1914 1071 906 757 670 85
BsmI GAATG_Cn'
Cuts at: 6358 6752 6845 6358
Size: 394 93 8754
Fragments arranged by size:
8754 394 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2469 5601 2469
Size: 3132 6109
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2095 2289 2433 2875 3055 3787
Size: 399 279 26 194 144 442 180 732
Cuts at: 3787 3812 4718 5388 6560 7302 7387 8458 1391
Size: 25 906 670 1172 742 85 1071 2174
Fragments arranged by size:
2174 1172 1071 906 742 732 670 442
399 279 194 180 144 85 26 25
BspEI T'CCGG_A
Cuts at: 8904 8904
Size: 9241
BspHI T'CATG_A
Cuts at: 3496 7130 8860 3496
Size: 3634 1730 3877
Fragments arranged by size:
3877 3634 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5620 6596 844
Size: 4776 976 3489
Fragments arranged by size:
4776 3489 976
BsrBI CCG'CTC
Cuts at: 1394 1823 2164 2236 7128 1394
Size: 429 341 72 4892 3507
Fragments arranged by size:
4892 3507 429 341 72
BsrDI GCAATG_nn'
Cuts at: 65 1248 5592 7736 7910 65
Size: 1183 4344 2144 174 1396
Fragments arranged by size:
4344 2144 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2257 2312 2483 7885 8072 1700
Size: 110 447 55 171 5402 187 2869
Fragments arranged by size:
5402 2869 447 187 171 110 55
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9241
BssHII G'CGCG_C
Cuts at: 1925 2109 1925
Size: 184 9057
BssSI C'ACGA_G
Cuts at: 3512 7301 8313 3512
Size: 3789 1012 4440
Fragments arranged by size:
4440 3789 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6410 794
Size: 5616 3625
BstBI TT'CG_AA
Cuts at: 8045 8045
Size: 9241
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9241
BstXI CCAn_nnnn'nTGG
Cuts at: 3129 4615 3129
Size: 1486 7755
BstYI r'GATC_y
Cuts at: 1972 2669 3479 3491 4184 4458 5227 6571 6938
Size: 697 810 12 693 274 769 1344 367
Cuts at: 6938 7323 7340 8792 8878 8890 9236 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1344 810 769 697 693 385
367 346 274 86 17 12 12
Bsu36I CC'TnA_GG
Cuts at: 2070 2907 3755 6258 6483 2070
Size: 837 848 2503 225 4828
Fragments arranged by size:
4828 2503 848 837 225
BtgI C'CryG_G
Cuts at: 513 1065 2004 2115 2124 2289 2344 2359 3680
Size: 552 939 111 9 165 55 15 1321
Cuts at: 3680 5580 6067 6347 513
Size: 1900 487 280 3407
Fragments arranged by size:
3407 1900 1321 939 552 487 280 165
111 55 15 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8502
Fragments arranged by size:
8502 683 56
BtsI GCAGTG_nn'
Cuts at: 3412 3838 4992 6757 7551 7571 3412
Size: 426 1154 1765 794 20 5082
Fragments arranged by size:
5082 1765 1154 794 426 20
ClaI AT'CG_AT
Cuts at: 1554 6419 1554
Size: 4865 4376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3510 3545 529
Size: 35 2946 35 6225
Fragments arranged by size:
6225 2946 35 35
DraI TTT'AAA
Cuts at: 3144 3969 6545 3144
Size: 825 2576 5840
Fragments arranged by size:
5840 2576 825
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2275 4893 8248 809
Size: 507 959 2618 3355 1802
Fragments arranged by size:
3355 2618 1802 959 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2424 4270 4797
Size: 54 1425 50 275 18 594 1846 527
Cuts at: 4797 6688 7577 9206 8
Size: 1891 889 1629 43
Fragments arranged by size:
1891 1846 1629 1425 889 594 527 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2424 6688 1537
Size: 275 612 4264 4090
Fragments arranged by size:
4264 4090 612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2243 2863 3312 3429 5877 5945 5972 6465
Size: 890 620 449 117 2448 68 27 493
Cuts at: 6465 7171 1353
Size: 706 3423
Fragments arranged by size:
3423 2448 890 706 620 493 449 117
68 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2100 2183 7828 8202 8348 387
Size: 1064 138 511 83 5645 374 146 1280
Fragments arranged by size:
5645 1280 1064 511 374 146 138 83
EcoICRI GAG'CTC
Cuts at: 5386 5386
Size: 9241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2591 2685 2787 3099 4016 4028 5006
Size: 393 959 94 102 312 917 12 978
Cuts at: 5006 5149 5377 5574 6533 7304 7901 8688 8956
Size: 143 228 197 959 771 597 787 268
Cuts at: 8956 1239
Size: 1524
Fragments arranged by size:
1524 978 959 959 917 787 771 597
393 312 268 228 197 143 102 94
12
EcoNI CCTnn'n_nnAGG
Cuts at: 2073 5202 2073
Size: 3129 6112
EcoO109I rG'GnC_Cy
Cuts at: 2630 2972 3783 3784 4814 5404 2630
Size: 342 811 1 1030 590 6467
Fragments arranged by size:
6467 1030 811 590 342 1
EcoRI G'AATT_C
Cuts at: 6550 6928 6550
Size: 378 8863
EcoRV GAT'ATC
Cuts at: 5650 5650
Size: 9241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2742 2892 3386 4283 2742
Size: 150 494 897 7700
Fragments arranged by size:
7700 897 494 150
FspI TGC'GCA
Cuts at: 2192 7747 2192
Size: 5555 3686
HaeII r_GCGC'y
Cuts at: 1678 2055 2370 2396 2409 2450 8388 1678
Size: 377 315 26 13 41 5938 2531
Fragments arranged by size:
5938 2531 377 315 41 26 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2521 2553 2876 2908 3193
Size: 32 135 32 949 32 323 32 285
Cuts at: 3193 3225 5239 5271 5358 5390 5634 5666 5887
Size: 32 2014 32 87 32 244 32 221
Cuts at: 5887 5919 7882 7914 7956 7988 1373
Size: 32 1963 32 42 32 2626
Fragments arranged by size:
2626 2014 1963 949 323 285 244 221
135 87 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2929 4643 4956 5744 6275 6585
Size: 988 281 991 1714 313 788 531 310
Cuts at: 6585 6831 669
Size: 246 3079
Fragments arranged by size:
3079 1714 991 988 788 531 313 310
281 246
HindIII A'AGCT_T
Cuts at: 748 5565 748
Size: 4817 4424
HpaI GTT'AAC
Cuts at: 2929 6831 2929
Size: 3902 5339
KasI G'GCGC_C
Cuts at: 2051 2366 2392 2405 2446 2051
Size: 315 26 13 41 8846
Fragments arranged by size:
8846 315 41 26 13
KpnI G_GTAC'C
Cuts at: 6566 6566
Size: 9241
MfeI C'AATT_G
Cuts at: 4252 5574 6240 6840 4252
Size: 1322 666 600 6653
Fragments arranged by size:
6653 1322 666 600
MluI A'CGCG_T
Cuts at: 9117 9117
Size: 9241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2589 4104 4281 8355 8539 1305
Size: 1284 1515 177 4074 184 2007
Fragments arranged by size:
4074 2007 1515 1284 184 177
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4799 10
Size: 54 1425 343 2967 4452
Fragments arranged by size:
4452 2967 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4556 5585 5927 6108 7199 7558 7717
Size: 648 3390 1029 342 181 1091 359 159
Cuts at: 7717 518
Size: 2042
Fragments arranged by size:
3390 2042 1091 1029 648 359 342 181
159
MspA1I CmG'CkG
Cuts at: 1606 2117 2126 2151 2188 2221 2291 2300 2361
Size: 511 9 25 37 33 70 9 61
Cuts at: 2361 2403 2423 2474 2693 3048 4105 4988 5626
Size: 42 20 51 219 355 1057 883 638
Cuts at: 5626 6657 7334 8482 8727 1606
Size: 1031 677 1148 245 2120
Fragments arranged by size:
2120 1148 1057 1031 883 677 638 511
355 245 219 70 61 51 42 37
33 25 20 9 9
NaeI GCC'GGC
Cuts at: 2259 2259
Size: 9241
NarI GG'CG_CC
Cuts at: 2052 2367 2393 2406 2447 2052
Size: 315 26 13 41 8846
Fragments arranged by size:
8846 315 41 26 13
NcoI C'CATG_G
Cuts at: 513 1065 2004 3680 5580 6347 513
Size: 552 939 1676 1900 767 3407
Fragments arranged by size:
3407 1900 1676 939 767 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9241
NgoMIV G'CCGG_C
Cuts at: 2257 2257
Size: 9241
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9241
NotI GC'GGCC_GC
Cuts at: 6688 6688
Size: 9241
NspI r_CATG'y
Cuts at: 1132 1229 3250 3355 3421 3909 6599 8144 1132
Size: 97 2021 105 66 488 2690 1545 2229
Fragments arranged by size:
2690 2229 2021 1545 488 105 97 66
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9241
PciI A'CATG_T
Cuts at: 8140 8140
Size: 9241
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2326 4435 1108
Size: 1218 2109 5914
Fragments arranged by size:
5914 2109 1218
PfoI T'CCnGG_A
Cuts at: 2701 2803 2701
Size: 102 9139
PmeI GTTT'AAAC
Cuts at: 6545 6545
Size: 9241
PmlI CAC'GTG
Cuts at: 3056 5943 3056
Size: 2887 6354
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3184 4756 4788 5324 5356 6048 6080 7310
Size: 32 1572 32 536 32 692 32 1230
Cuts at: 7310 7342 8823 8855 3152
Size: 32 1481 32 3538
Fragments arranged by size:
3538 1572 1481 1230 692 536 32 32
32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4814 5404 4814
Size: 590 8651
PshAI GACnn'nnGTC
Cuts at: 1613 5831 1613
Size: 4218 5023
PsiI TTA'TAA
Cuts at: 6811 6811
Size: 9241
PspOMI G'GGCC_C
Cuts at: 3783 3783
Size: 9241
PspXI vC'TCGA_Gb
Cuts at: 2410 2410
Size: 9241
PstI C_TGCA'G
Cuts at: 830 2473 2964 3047 3395 3914 5042 5631 6181
Size: 1643 491 83 348 519 1128 589 550
Cuts at: 6181 6593 830
Size: 412 3478
Fragments arranged by size:
3478 1643 1128 589 550 519 491 412
348 83
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9241
PvuII CAG'CTG
Cuts at: 2188 2221 2693 3048 4105 4988 5626 2188
Size: 33 472 355 1057 883 638 5803
Fragments arranged by size:
5803 1057 883 638 472 355 33
SacI G_AGCT'C
Cuts at: 5388 5388
Size: 9241
SacII CC_GC'GG
Cuts at: 2118 2127 2292 2362 2118
Size: 9 165 70 8997
Fragments arranged by size:
8997 165 70 9
SalI G'TCGA_C
Cuts at: 1936 6583 1936
Size: 4647 4594
SapI GCTCTTCn'nnn_
Cuts at: 2243 2863 5945 6465 2243
Size: 620 3082 520 5019
Fragments arranged by size:
5019 3082 620 520
SbfI CC_TGCA'GG
Cuts at: 6593 6593
Size: 9241
ScaI AGT'ACT
Cuts at: 3280 4339 7489 9201 3280
Size: 1059 3150 1712 3320
Fragments arranged by size:
3320 3150 1712 1059
SfcI C'TryA_G
Cuts at: 826 1045 2469 2960 3043 3391 3505 3910 4488
Size: 219 1424 491 83 348 114 405 578
Cuts at: 4488 5038 5293 5627 6177 6589 7724 8405 8596
Size: 550 255 334 550 412 1135 681 191
Cuts at: 8596 826
Size: 1471
Fragments arranged by size:
1471 1424 1135 681 578 550 550 491
412 405 348 334 255 219 191 114
83
SfoI GGC'GCC
Cuts at: 2053 2368 2394 2407 2448 2053
Size: 315 26 13 41 8846
Fragments arranged by size:
8846 315 41 26 13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9241
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9241
SmaI CCC'GGG
Cuts at: 1872 2049 2434 6568 1872
Size: 177 385 4134 4545
Fragments arranged by size:
4545 4134 385 177
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2096 2410 3196 4465 5784 7345
Size: 197 926 153 314 786 1269 1319 1561
Cuts at: 7345 8246 8508 820
Size: 901 262 1553
Fragments arranged by size:
1561 1553 1319 1269 926 901 786 314
262 197 153
SnaBI TAC'GTA
Cuts at: 493 7032 493
Size: 6539 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9241
SphI G_CATG'C
Cuts at: 1132 3355 3909 6599 1132
Size: 2223 554 2690 3774
Fragments arranged by size:
3774 2690 2223 554
SspI AAT'ATT
Cuts at: 5 52 7165 5
Size: 47 7113 2081
Fragments arranged by size:
7113 2081 47
StuI AGG'CCT
Cuts at: 1517 5754 1517
Size: 4237 5004
StyI C'CwwG_G
Cuts at: 513 1065 2004 3680 5580 5755 6347 6680 513
Size: 552 939 1676 1900 175 592 333 3074
Fragments arranged by size:
3074 1900 1676 939 592 552 333 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 7268 7285 7438 7623 8068 325
Size: 1255 5688 17 153 185 445 1498
Fragments arranged by size:
5688 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2952 3278 4337
Size: 274 80 33 51 1443 975 326 1059
Cuts at: 4337 7487 9199 96
Size: 3150 1712 138
Fragments arranged by size:
3150 1712 1443 1059 975 326 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3314 3346 3882 3914 9201 9233 3314
Size: 32 536 32 5287 32 3322
Fragments arranged by size:
5287 3322 536 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2751 3941 1316
Size: 1435 1190 6616
Fragments arranged by size:
6616 1435 1190
XbaI T'CTAG_A
Cuts at: 3725 6577 3725
Size: 2852 6389
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3776 4436 4681 3776
Size: 660 245 8336
Fragments arranged by size:
8336 660 245
XhoI C'TCGA_G
Cuts at: 1943 2410 1943
Size: 467 8774
XmaI C'CCGG_G
Cuts at: 1870 2047 2432 6566 1870
Size: 177 385 4134 4545
Fragments arranged by size:
4545 4134 385 177
XmnI GAAnn'nnTTC
Cuts at: 5047 7370 5047
Size: 2323 6918
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7049 276
Size: 53 83 186 936 5515 2468
Fragments arranged by size:
5515 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BfrBI BsiWI BstZ17I DraIII
FseI FspAI NruI NsiI PacI PsrI RsrII SanDI
SexAI SfiI SrfI SwaI