Restriction map for
pFN21AE3471
(Circular) (Six-base) MAPSORT of: pFN21AE3471.seq Check: 4594 from: 1 to: 9061
pFN21AE3471 9061 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6871 278
Size: 53 83 186 936 5335 2468
Fragments arranged by size:
5335 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4613 6404 1937
Size: 2676 1791 4594
Fragments arranged by size:
4594 2676 1791
Acc65I G'GTAC_C
Cuts at: 4638 6382 4638
Size: 1744 7317
AclI AA'CG_TT
Cuts at: 1563 7188 7561 1563
Size: 5625 373 3063
Fragments arranged by size:
5625 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2230 3359 3617 3876 4967 5805 7124 8508
Size: 598 1129 258 259 1091 838 1319 1384
Cuts at: 8508 8776 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1319 1129 1091 838 598 268
259 258
AfeI AGC'GCT
Cuts at: 2785 4961 2785
Size: 2176 6885
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8864
AflIII A'CryG_T
Cuts at: 1203 3147 3799 5811 7960 8937 1203
Size: 1944 652 2012 2149 977 1327
Fragments arranged by size:
2149 2012 1944 1327 977 652
AgeI A'CCGG_T
Cuts at: 7892 7892
Size: 9061
AhdI GACnn_n'nnGTC
Cuts at: 7790 7790
Size: 9061
AleI CACnn'nnGTG
Cuts at: 1166 5504 5810 1166
Size: 4338 306 4417
Fragments arranged by size:
4417 4338 306
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9061
AlwNI CAG_nnn'CTG
Cuts at: 2149 2473 3007 3177 5636 6038 6804 8376 2149
Size: 324 534 170 2459 402 766 1572 2834
Fragments arranged by size:
2834 2459 1572 766 534 402 324 170
ApaI G_GGCC'C
Cuts at: 2258 2331 2258
Size: 73 8988
ApaLI G'TGCA_C
Cuts at: 7118 8274 7118
Size: 1156 7905
ApoI r'AATT_y
Cuts at: 1493 4115 4266 4892 5298 5583 5935 6370 6596
Size: 2622 151 626 406 285 352 435 226
Cuts at: 6596 6748 8857 1493
Size: 152 2109 1697
Fragments arranged by size:
2622 2109 1697 626 435 406 352 285
226 152 151
AseI AT'TA_AT
Cuts at: 160 1032 4778 7615 160
Size: 872 3746 2837 1606
Fragments arranged by size:
3746 2837 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9061
AvaI C'yCGr_G
Cuts at: 1870 1943 2251 3079 5609 6386 1870
Size: 73 308 828 2530 777 4545
Fragments arranged by size:
4545 2530 828 777 308 73
BamHI G'GATC_C
Cuts at: 4948 5843 6391 6758 4948
Size: 895 548 367 7251
Fragments arranged by size:
7251 895 548 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2561 3180 4638 5233 6382
Size: 325 217 627 774 619 1458 595 1149
Cuts at: 6382 7837 618
Size: 1455 1842
Fragments arranged by size:
1842 1458 1455 1149 774 627 619 595
325 217
BanII G_rGCy'C
Cuts at: 1391 2258 2331 2456 3086 6380 1391
Size: 867 73 125 630 3294 4072
Fragments arranged by size:
4072 3294 867 630 125 73
BbeI G_GCGC'C
Cuts at: 3184 3184
Size: 9061
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2356 3929 5089 5271 928
Size: 971 457 1573 1160 182 4718
Fragments arranged by size:
4718 1573 1160 971 457 182
BbvCI CC'TCA_GC
Cuts at: 2106 2951 3444 5997 2106
Size: 845 493 2553 5170
Fragments arranged by size:
5170 2553 845 493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4856 4890 7252 7286 1903
Size: 34 50 34 2835 34 2362 34 3678
Fragments arranged by size:
3678 2835 2362 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4721 6296 6953 8163 4721
Size: 1575 657 1210 5619
Fragments arranged by size:
5619 1575 1210 657
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9061
BfrBI ATG'CAT
Cuts at: 6253 6253
Size: 9061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2228 2495 2718
Size: 107 122 71 631 605 556 267 223
Cuts at: 2718 5354 7672 136
Size: 2636 2318 1525
Fragments arranged by size:
2636 2318 1525 631 605 556 267 223
122 107 71
BglII A'GATC_T
Cuts at: 3266 5032 5644 9056 3266
Size: 1766 612 3412 3271
Fragments arranged by size:
3412 3271 1766 612
BlpI GC'TnA_GC
Cuts at: 5403 5745 6478 5403
Size: 342 733 7986
Fragments arranged by size:
7986 733 342
Bme1580I G_kGCm'C
Cuts at: 1790 2258 2331 7122 8278 1790
Size: 468 73 4791 1156 2573
Fragments arranged by size:
4791 2573 1156 468 73
BmgBI CAC'GTC
Cuts at: 1318 3148 1318
Size: 1830 7231
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2452 2969 4102
Size: 416 53 83 399 24 1035 517 1133
Cuts at: 4102 4670 6021 6792 7750 442
Size: 568 1351 771 958 1753
Fragments arranged by size:
1753 1351 1133 1035 958 771 568 517
416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 4206 5472 7721 1239
Size: 1046 1921 1266 2249 2579
Fragments arranged by size:
2579 2249 1921 1266 1046
Bpu10I CC'TnA_GC
Cuts at: 2048 2106 2951 3444 3963 5997 2048
Size: 58 845 493 519 2034 5112
Fragments arranged by size:
5112 2034 845 519 493 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3568 7186 8051 8349 3568
Size: 3618 865 298 4280
Fragments arranged by size:
4280 3618 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7724 882
Size: 617 6225 2219
Fragments arranged by size:
6225 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5812 6852 493
Size: 711 4608 1040 2702
Fragments arranged by size:
4608 2702 1040 711
BsaBI GATnn'nnATC
Cuts at: 9055 9055
Size: 9061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2320 3181 6868 7250
Size: 53 83 186 936 787 861 3687 382
Cuts at: 7250 7885 275
Size: 635 1451
Fragments arranged by size:
3687 1451 936 861 787 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5503 7494 7892 8166 8313 8724 5503
Size: 1991 398 274 147 411 5840
Fragments arranged by size:
5840 1991 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2051 2081 4179 4209 4789
Size: 30 165 30 648 30 2098 30 580
Cuts at: 4789 4819 5019 5049 1178
Size: 30 200 30 5190
Fragments arranged by size:
5190 2098 648 580 200 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2224 2227 2542 2545 3025 3990 1186
Size: 1038 3 315 3 480 965 6257
Fragments arranged by size:
6257 1038 965 480 315 3 3
BseYI C'CCAG_C
Cuts at: 2029 2052 2376 2479 2565 3026 3107 6242 8264
Size: 23 324 103 86 461 81 3135 2022
Cuts at: 8264 8760 2029
Size: 496 2330
Fragments arranged by size:
3135 2330 2022 496 461 324 103 86
81 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4948 5381 1163
Size: 573 3212 433 4843
Fragments arranged by size:
4843 3212 573 433
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2205 2773 4610 6511 7272 8300
Size: 275 186 204 568 1837 1901 761 1028
Cuts at: 8300 1540
Size: 2301
Fragments arranged by size:
2301 1901 1837 1028 761 568 275 204
186
BsiHKAI G_wGCw'C
Cuts at: 2456 2652 3213 7122 7207 8278 2456
Size: 196 561 3909 85 1071 3239
Fragments arranged by size:
3909 3239 1071 561 196 85
BsmI GAATG_Cn'
Cuts at: 3259 6572 6665 3259
Size: 3313 93 5655
Fragments arranged by size:
5655 3313 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2258 2331 2456 2652 3086 3213 6380
Size: 399 468 73 125 196 434 127 3167
Cuts at: 6380 7122 7207 8278 1391
Size: 742 85 1071 2174
Fragments arranged by size:
3167 2174 1071 742 468 434 399 196
127 125 85 73
BspEI T'CCGG_A
Cuts at: 8724 8724
Size: 9061
BspHI T'CATG_A
Cuts at: 5056 6950 8680 5056
Size: 1894 1730 5437
Fragments arranged by size:
5437 1894 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6416 844
Size: 5572 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2131 2748 3102 3136 3186 6948 1394
Size: 429 308 617 354 34 50 3762 3507
Fragments arranged by size:
3762 3507 617 429 354 308 50 34
BsrDI GCAATG_nn'
Cuts at: 65 1248 5884 7556 7730 65
Size: 1183 4636 1672 174 1396
Fragments arranged by size:
4636 1672 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2652 5574 7705 7892 1700
Size: 110 842 2922 2131 187 2869
Fragments arranged by size:
2922 2869 2131 842 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9061
BssHII G'CGCG_C
Cuts at: 1925 2296 1925
Size: 371 8690
BssSI C'ACGA_G
Cuts at: 3381 7121 8133 3381
Size: 3740 1012 4309
Fragments arranged by size:
4309 3740 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2149 2473 794
Size: 1355 324 7382
Fragments arranged by size:
7382 1355 324
BstBI TT'CG_AA
Cuts at: 5282 7865 5282
Size: 2583 6478
BstEII G'GTnAC_C
Cuts at: 1184 6351 1184
Size: 5167 3894
BstXI CCAn_nnnn'nTGG
Cuts at: 6287 6287
Size: 9061
BstYI r'GATC_y
Cuts at: 1972 3266 3293 4948 5032 5254 5547 5644 5843
Size: 1294 27 1655 84 222 293 97 199
Cuts at: 5843 6391 6758 7143 7160 8612 8698 8710 9056
Size: 548 367 385 17 1452 86 12 346
Cuts at: 9056 1972
Size: 1977
Fragments arranged by size:
1977 1655 1452 1294 548 385 367 346
293 222 199 97 86 84 27 17
12
BstZ17I GTA'TAC
Cuts at: 4614 4614
Size: 9061
Bsu36I CC'TnA_GG
Cuts at: 2925 3008 2925
Size: 83 8978
BtgI C'CryG_G
Cuts at: 513 1065 2226 6115 6156 513
Size: 552 1161 3889 41 3418
Fragments arranged by size:
3889 3418 1161 552 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8322
Fragments arranged by size:
8322 683 56
BtsI GCAGTG_nn'
Cuts at: 2626 4818 4965 6151 6577 7371 7391 2626
Size: 2192 147 1186 426 794 20 4296
Fragments arranged by size:
4296 2192 1186 794 426 147 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9026
DraI TTT'AAA
Cuts at: 3600 6365 3600
Size: 2765 6296
DraIII CAC_nnn'GTG
Cuts at: 5240 5240
Size: 9061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2857 8068 809
Size: 507 1541 5211 1802
Fragments arranged by size:
5211 1802 1541 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2202 2623 2770
Size: 54 1425 50 275 18 372 421 147
Cuts at: 2770 2790 4607 6508 7397 9026 8
Size: 20 1817 1901 889 1629 43
Fragments arranged by size:
1901 1817 1629 1425 889 421 372 275
147 54 50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2202 2770 4607 6508 1537
Size: 275 390 568 1837 1901 4090
Fragments arranged by size:
4090 1901 1837 568 390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 3335 3636 4730 4986 5430 5854 5863
Size: 833 1149 301 1094 256 444 424 9
Cuts at: 5863 6991 1353
Size: 1128 3423
Fragments arranged by size:
3423 1149 1128 1094 833 444 424 301
256 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2072 2363 7648 8022 8168 387
Size: 1064 138 483 291 5285 374 146 1280
Fragments arranged by size:
5285 1280 1064 483 374 291 146 138
EcoICRI GAG'CTC
Cuts at: 2454 2454
Size: 9061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2230 2285 3359 3617 3876 4206 4967
Size: 393 598 55 1074 258 259 330 761
Cuts at: 4967 5472 5805 7124 7721 8508 8776 1239
Size: 505 333 1319 597 787 268 1524
Fragments arranged by size:
1524 1319 1074 787 761 598 597 505
393 333 330 268 259 258 55
EcoNI CCTnn'n_nnAGG
Cuts at: 6279 6279
Size: 9061
EcoO109I rG'GnC_Cy
Cuts at: 2044 2255 2328 2595 2994 6284 2044
Size: 211 73 267 399 3290 4821
Fragments arranged by size:
4821 3290 399 267 211 73
EcoRI G'AATT_C
Cuts at: 5298 5583 6370 6748 5298
Size: 285 787 378 7611
Fragments arranged by size:
7611 787 378 285
EcoRV GAT'ATC
Cuts at: 4123 4123
Size: 9061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4252 4516 5854 4252
Size: 264 1338 7459
Fragments arranged by size:
7459 1338 264
FspI TGC'GCA
Cuts at: 7567 7567
Size: 9061
HaeII r_GCGC'y
Cuts at: 1678 2030 2641 2787 3184 4963 8208 1678
Size: 352 611 146 397 1779 3245 2531
Fragments arranged by size:
3245 2531 1779 611 397 352 146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2438 2470 2813 2845 3695
Size: 32 135 32 866 32 343 32 850
Cuts at: 3695 3727 4789 4821 5776 5808 5933 5965 6160
Size: 32 1062 32 955 32 125 32 195
Cuts at: 6160 6192 7702 7734 7776 7808 1373
Size: 32 1510 32 42 32 2626
Fragments arranged by size:
2626 1510 1062 955 866 850 343 195
135 125 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5385 6405 6651 669
Size: 988 281 3447 1020 246 3079
Fragments arranged by size:
3447 3079 1020 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3468 748
Size: 2720 6341
HpaI GTT'AAC
Cuts at: 6651 6651
Size: 9061
KasI G'GCGC_C
Cuts at: 3180 3180
Size: 9061
KpnI G_GTAC'C
Cuts at: 4642 6386 4642
Size: 1744 7317
MfeI C'AATT_G
Cuts at: 6660 6660
Size: 9061
MluI A'CGCG_T
Cuts at: 8937 8937
Size: 9061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2049 2565 3676 8175 8359 1305
Size: 744 516 1111 4499 184 2007
Fragments arranged by size:
4499 2007 1111 744 516 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7239
Fragments arranged by size:
7239 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4431 5504 5810 5985 7019 7378 7537
Size: 648 3265 1073 306 175 1034 359 159
Cuts at: 7537 518
Size: 2042
Fragments arranged by size:
3265 2042 1073 1034 648 359 306 175
159
MspA1I CmG'CkG
Cuts at: 1606 2119 2228 2431 2535 2569 2718 2766 3177
Size: 513 109 203 104 34 149 48 411
Cuts at: 3177 3356 3955 4913 5206 5737 6477 7154 8302
Size: 179 599 958 293 531 740 677 1148
Cuts at: 8302 8547 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 958 740 677 599 531 513
411 293 245 203 179 149 109 104
48 34
NarI GG'CG_CC
Cuts at: 3181 3181
Size: 9061
NcoI C'CATG_G
Cuts at: 513 1065 6115 513
Size: 552 5050 3459
Fragments arranged by size:
5050 3459 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9061
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9061
NotI GC'GGCC_GC
Cuts at: 2770 6508 2770
Size: 3738 5323
NsiI A_TGCA'T
Cuts at: 6255 6255
Size: 9061
NspI r_CATG'y
Cuts at: 1132 1229 3572 3803 4939 5675 6419 7964 1132
Size: 97 2343 231 1136 736 744 1545 2229
Fragments arranged by size:
2343 2229 1545 1136 744 736 231 97
PasI CC'CwG_GG
Cuts at: 1348 5182 1348
Size: 3834 5227
PciI A'CATG_T
Cuts at: 3799 7960 3799
Size: 4161 4900
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9061
PmeI GTTT'AAAC
Cuts at: 6365 6365
Size: 9061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7130 7162 8643 8675 7130
Size: 32 1481 32 7516
Fragments arranged by size:
7516 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 6284 6284
Size: 9061
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9061
PsiI TTA'TAA
Cuts at: 6631 6631
Size: 9061
PspOMI G'GGCC_C
Cuts at: 2254 2327 2254
Size: 73 8988
PstI C_TGCA'G
Cuts at: 830 2139 3427 4813 4912 6413 830
Size: 1309 1288 1386 99 1501 3478
Fragments arranged by size:
3478 1501 1386 1309 1288 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9061
PvuII CAG'CTG
Cuts at: 3177 3356 4913 5206 5737 3177
Size: 179 1557 293 531 6501
Fragments arranged by size:
6501 1557 531 293 179
SacI G_AGCT'C
Cuts at: 2456 2456
Size: 9061
SacII CC_GC'GG
Cuts at: 2229 2229
Size: 9061
SalI G'TCGA_C
Cuts at: 1936 6403 1936
Size: 4467 4594
SapI GCTCTTCn'nnn_
Cuts at: 2186 4986 2186
Size: 2800 6261
SbfI CC_TGCA'GG
Cuts at: 6413 6413
Size: 9061
ScaI AGT'ACT
Cuts at: 5968 6172 7309 9021 5968
Size: 204 1137 1712 6008
Fragments arranged by size:
6008 1712 1137 204
SexAI A'CCwGG_T
Cuts at: 5236 5236
Size: 9061
SfcI C'TryA_G
Cuts at: 826 1045 2135 2932 3088 3112 3163 3199 3423
Size: 219 1090 797 156 24 51 36 224
Cuts at: 3423 4809 4908 5962 6409 7544 8225 8416 826
Size: 1386 99 1054 447 1135 681 191 1471
Fragments arranged by size:
1471 1386 1135 1090 1054 797 681 447
224 219 191 156 99 51 36 24
SfoI GGC'GCC
Cuts at: 3182 3182
Size: 9061
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9061
SgrAI Cr'CCGG_yG
Cuts at: 1700 2652 1700
Size: 952 8109
SmaI CCC'GGG
Cuts at: 1872 2253 6388 1872
Size: 381 4135 4545
Fragments arranged by size:
4545 4135 381
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3583 7165 8066 8328 820
Size: 197 926 1640 3582 901 262 1553
Fragments arranged by size:
3582 1640 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6852 493
Size: 6359 2702
SpeI A'CTAG_T
Cuts at: 152 3509 152
Size: 3357 5704
SphI G_CATG'C
Cuts at: 1132 6419 1132
Size: 5287 3774
SrfI GCCC'GGGC
Cuts at: 2253 2253
Size: 9061
SspI AAT'ATT
Cuts at: 5 52 3889 6985 5
Size: 47 3837 3096 2081
Fragments arranged by size:
3837 3096 2081 47
StuI AGG'CCT
Cuts at: 1517 2687 2900 2930 4042 1517
Size: 1170 213 30 1112 6536
Fragments arranged by size:
6536 1170 1112 213 30
StyI C'CwwG_G
Cuts at: 513 1065 2862 2895 3456 3492 3783 4714 4944
Size: 552 1797 33 561 36 291 931 230
Cuts at: 4944 6115 6280 6500 513
Size: 1171 165 220 3074
Fragments arranged by size:
3074 1797 1171 931 561 552 291 230
220 165 36 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2579 2933 7088 7105 7258 7443 7888
Size: 1255 999 354 4155 17 153 185 445
Cuts at: 7888 325
Size: 1498
Fragments arranged by size:
4155 1498 1255 999 445 354 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5966 6170 7307
Size: 274 80 33 51 1443 3989 204 1137
Cuts at: 7307 9019 96
Size: 1712 138
Fragments arranged by size:
3989 1712 1443 1137 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5019 5051 9021 9053 5019
Size: 32 3970 32 5027
Fragments arranged by size:
5027 3970 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2854 1316
Size: 1538 7523
XbaI T'CTAG_A
Cuts at: 3290 5647 6397 3290
Size: 2357 750 5954
Fragments arranged by size:
5954 2357 750
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9061
XmaI C'CCGG_G
Cuts at: 1870 2251 6386 1870
Size: 381 4135 4545
Fragments arranged by size:
4545 4135 381
XmnI GAAnn'nnTTC
Cuts at: 3889 4236 5896 7190 3889
Size: 347 1660 1294 5760
Fragments arranged by size:
5760 1660 1294 347
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6869 276
Size: 53 83 186 936 5335 2468
Fragments arranged by size:
5335 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BplI BsiWI BsmBI
FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI
PspXI PsrI RsrII SanDI SfiI SwaI XcmI