Restriction map for
pFN21AE3469
(Circular) (Six-base) MAPSORT of: pFN21AE3469.seq Check: 1341 from: 1 to: 9058
pFN21AE3469 9058 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3060 3060
Size: 9058
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6868 278
Size: 53 83 186 936 5332 2468
Fragments arranged by size:
5332 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2523 6401 1937
Size: 586 3878 4594
Fragments arranged by size:
4594 3878 586
Acc65I G'GTAC_C
Cuts at: 2051 6379 2051
Size: 4328 4730
AclI AA'CG_TT
Cuts at: 1563 7185 7558 1563
Size: 5622 373 3063
Fragments arranged by size:
5622 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2513 2728 3536 3612 5349 5511 6063 6285
Size: 881 215 808 76 1737 162 552 222
Cuts at: 6285 7121 8505 8773 1632
Size: 836 1384 268 1917
Fragments arranged by size:
1917 1737 1384 881 836 808 552 268
222 215 162 76
AfeI AGC'GCT
Cuts at: 2441 2441
Size: 9058
AflII C'TTAA_G
Cuts at: 820 1017 2056 2132 820
Size: 197 1039 76 7746
Fragments arranged by size:
7746 1039 197 76
AflIII A'CryG_T
Cuts at: 1203 3177 4158 7957 8934 1203
Size: 1974 981 3799 977 1327
Fragments arranged by size:
3799 1974 1327 981 977
AgeI A'CCGG_T
Cuts at: 7889 7889
Size: 9058
AhdI GACnn_n'nnGTC
Cuts at: 7787 7787
Size: 9058
AleI CACnn'nnGTG
Cuts at: 1166 5150 1166
Size: 3984 5074
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2460 2858 2906 1184
Size: 1276 398 48 7336
Fragments arranged by size:
7336 1276 398 48
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4145 4177 4145
Size: 32 9026
AlwNI CAG_nnn'CTG
Cuts at: 3929 4826 6801 8373 3929
Size: 897 1975 1572 4614
Fragments arranged by size:
4614 1975 1572 897
ApaI G_GGCC'C
Cuts at: 4373 4373
Size: 9058
ApaLI G'TGCA_C
Cuts at: 3047 4525 4635 7115 8271 3047
Size: 1478 110 2480 1156 3834
Fragments arranged by size:
3834 2480 1478 1156 110
ApoI r'AATT_y
Cuts at: 1493 2127 2142 2213 5268 5612 6367 6593 6745
Size: 634 15 71 3055 344 755 226 152
Cuts at: 6745 8854 1493
Size: 2109 1697
Fragments arranged by size:
3055 2109 1697 755 634 344 226 152
71 15
AseI AT'TA_AT
Cuts at: 160 1032 7612 160
Size: 872 6580 1606
Fragments arranged by size:
6580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9058
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 3219 3394 5179 6383 1870
Size: 73 67 1209 175 1785 1204 4545
Fragments arranged by size:
4545 1785 1209 1204 175 73 67
BamHI G'GATC_C
Cuts at: 2313 4563 5750 6388 6755 2313
Size: 2250 1187 638 367 4616
Fragments arranged by size:
4616 2250 1187 638 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2895 5082 6379 7834
Size: 325 217 627 264 844 2187 1297 1455
Cuts at: 7834 618
Size: 1842
Fragments arranged by size:
2187 1842 1455 1297 844 627 325 264
217
BanII G_rGCy'C
Cuts at: 1391 2730 3547 3572 4046 4373 4899 4931 4986
Size: 1339 817 25 474 327 526 32 55
Cuts at: 4986 5895 5915 6073 6155 6377 1391
Size: 909 20 158 82 222 4072
Fragments arranged by size:
4072 1339 909 817 526 474 327 222
158 82 55 32 25 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 2387 4594 5724 928
Size: 971 116 372 2207 1130 4262
Fragments arranged by size:
4262 2207 1130 971 372 116
BbvCI CC'TCA_GC
Cuts at: 3116 4247 3116
Size: 1131 7927
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2280 2314 2959 2993 7249 7283 1903
Size: 34 343 34 645 34 4256 34 3678
Fragments arranged by size:
4256 3678 645 343 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6950 8160 6950
Size: 1210 7848
BclI T'GATC_A
Cuts at: 1548 3318 1548
Size: 1770 7288
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7669 136
Size: 107 122 71 631 605 5997 1525
Fragments arranged by size:
5997 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3876 6046 9053 3876
Size: 2170 3007 3881
Fragments arranged by size:
3881 3007 2170
BlpI GC'TnA_GC
Cuts at: 2079 6475 2079
Size: 4396 4662
Bme1580I G_kGCm'C
Cuts at: 1790 3051 3228 4373 4529 4639 7119 8275 1790
Size: 1261 177 1145 156 110 2480 1156 2573
Fragments arranged by size:
2573 2480 1261 1156 1145 177 156 110
BmgBI CAC'GTC
Cuts at: 1318 3466 1318
Size: 2148 6910
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2661 3010 3017
Size: 416 53 83 399 24 1244 349 7
Cuts at: 3017 3259 4090 4702 6789 7747 442
Size: 242 831 612 2087 958 1753
Fragments arranged by size:
2087 1753 1244 958 831 612 416 399
349 242 83 53 24 7
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3898 5608 3898
Size: 1710 7348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2616 3044 3191 3561 3906 7718 1239
Size: 1377 428 147 370 345 3812 2579
Fragments arranged by size:
3812 2579 1377 428 370 345 147
Bpu10I CC'TnA_GC
Cuts at: 2318 3116 4247 2318
Size: 798 1131 7129
Fragments arranged by size:
7129 1131 798
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4515 5217 7183 8048 8346 4515
Size: 702 1966 865 298 5227
Fragments arranged by size:
5227 1966 865 702 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5307 7721 882
Size: 617 3808 2414 2219
Fragments arranged by size:
3808 2414 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6849 493
Size: 711 5645 2702
Fragments arranged by size:
5645 2702 711
BsaBI GATnn'nnATC
Cuts at: 2250 9052 2250
Size: 6802 2256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4902 6865 7247 7882
Size: 53 83 186 936 3369 1963 382 635
Cuts at: 7882 275
Size: 1451
Fragments arranged by size:
3369 1963 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2686 3504 7491 7889 8163 8310 8721 2686
Size: 818 3987 398 274 147 411 3023
Fragments arranged by size:
3987 3023 818 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3164 3194 4828 4858 1178
Size: 30 165 30 1761 30 1634 30 5378
Fragments arranged by size:
5378 1761 1634 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4187 5967 6134 1186
Size: 3001 1780 167 4110
Fragments arranged by size:
4110 3001 1780 167
BseYI C'CCAG_C
Cuts at: 3833 4682 5756 8261 8757 3833
Size: 849 1074 2505 496 4134
Fragments arranged by size:
4134 2505 1074 849 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2573 3014 3599 1163
Size: 573 837 441 585 6622
Fragments arranged by size:
6622 837 585 573 441
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6508 7269 8297 1540
Size: 275 186 4507 761 1028 2301
Fragments arranged by size:
4507 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2276 3051 4046 4529 4639 5895 6073 7119 7204
Size: 775 995 483 110 1256 178 1046 85
Cuts at: 7204 8275 2276
Size: 1071 3059
Fragments arranged by size:
3059 1256 1071 1046 995 775 483 178
110 85
BsmI GAATG_Cn'
Cuts at: 4476 5287 6569 6662 4476
Size: 811 1282 93 6872
Fragments arranged by size:
6872 1282 811 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2276 2730 3051 3228 3547 3572 4046
Size: 399 486 454 321 177 319 25 474
Cuts at: 4046 4373 4529 4639 4899 4931 4986 5895 5915
Size: 327 156 110 260 32 55 909 20
Cuts at: 5915 6073 6155 6377 7119 7204 8275 1391
Size: 158 82 222 742 85 1071 2174
Fragments arranged by size:
2174 1071 909 742 486 474 454 399
327 321 319 260 222 177 158 156
110 85 82 55 32 25 20
BspEI T'CCGG_A
Cuts at: 8721 8721
Size: 9058
BspHI T'CATG_A
Cuts at: 2086 2763 3001 6947 8677 2086
Size: 677 238 3946 1730 2467
Fragments arranged by size:
3946 2467 1730 677 238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2355 3060 3093 6413 844
Size: 1511 705 33 3320 3489
Fragments arranged by size:
3489 3320 1511 705 33
BsrBI CCG'CTC
Cuts at: 1394 1823 3111 6945 1394
Size: 429 1288 3834 3507
Fragments arranged by size:
3834 3507 1288 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7553 7727 65
Size: 1183 6305 174 1396
Fragments arranged by size:
6305 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4659 7702 7889 1700
Size: 110 2849 3043 187 2869
Fragments arranged by size:
3043 2869 2849 187 110
BsrGI T'GTAC_A
Cuts at: 96 3202 96
Size: 3106 5952
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9058
BssSI C'ACGA_G
Cuts at: 2549 4229 7118 8130 2549
Size: 1680 2889 1012 3477
Fragments arranged by size:
3477 2889 1680 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3055 794
Size: 2261 6797
BstBI TT'CG_AA
Cuts at: 3864 7862 3864
Size: 3998 5060
BstEII G'GTnAC_C
Cuts at: 1184 2342 1184
Size: 1158 7900
BstYI r'GATC_y
Cuts at: 1972 2245 2313 2678 3736 3751 3876 4201 4563
Size: 273 68 365 1058 15 125 325 362
Cuts at: 4563 5750 6046 6388 6755 7140 7157 8609 8695
Size: 1187 296 342 367 385 17 1452 86
Cuts at: 8695 8707 9053 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1187 1058 385 367 365 362
346 342 325 296 273 125 86 68
17 15 12
Bsu36I CC'TnA_GG
Cuts at: 2207 3741 6117 2207
Size: 1534 2376 5148
Fragments arranged by size:
5148 2376 1534
BtgI C'CryG_G
Cuts at: 513 1065 3981 4365 5919 513
Size: 552 2916 384 1554 3652
Fragments arranged by size:
3652 2916 1554 552 384
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8319
Fragments arranged by size:
8319 683 56
BtsI GCAGTG_nn'
Cuts at: 2830 4036 4602 5958 6574 7368 7388 2830
Size: 1206 566 1356 616 794 20 4500
Fragments arranged by size:
4500 1356 1206 794 616 566 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9023
DraI TTT'AAA
Cuts at: 4383 6362 4383
Size: 1979 7079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5715 8065 809
Size: 507 4399 2350 1802
Fragments arranged by size:
4399 2350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2850 3765 4362
Size: 54 1425 50 275 18 1020 915 597
Cuts at: 4362 6505 7394 9023 8
Size: 2143 889 1629 43
Fragments arranged by size:
2143 1629 1425 1020 915 889 597 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6505 1537
Size: 275 4693 4090
Fragments arranged by size:
4693 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2688 3813 3909 3984 4051 4296 4646 5187
Size: 1335 1125 96 75 67 245 350 541
Cuts at: 5187 5337 6189 6988 1353
Size: 150 852 799 3423
Fragments arranged by size:
3423 1335 1125 852 799 541 350 245
150 96 75 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7645 8019 8165 387
Size: 1064 138 6056 374 146 1280
Fragments arranged by size:
6056 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4044 5893 6071 4044
Size: 1849 178 7031
Fragments arranged by size:
7031 1849 178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2513 2616 2728 3044 3191 3536 3561
Size: 393 881 103 112 316 147 345 25
Cuts at: 3561 3612 3906 5349 5511 6063 6285 7121 7718
Size: 51 294 1443 162 552 222 836 597
Cuts at: 7718 8505 8773 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1443 881 836 787 597 552 393
345 316 294 268 222 162 147 112
103 51 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2552 5765 6146 2552
Size: 3213 381 5464
Fragments arranged by size:
5464 3213 381
EcoO109I rG'GnC_Cy
Cuts at: 2545 2938 3211 3745 4370 4667 6091 2545
Size: 393 273 534 625 297 1424 5512
Fragments arranged by size:
5512 1424 625 534 393 297 273
EcoRI G'AATT_C
Cuts at: 6367 6745 6367
Size: 378 8680
EcoRV GAT'ATC
Cuts at: 3066 3502 3066
Size: 436 8622
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2099 3541 4795 5570 5748 2099
Size: 1442 1254 775 178 5409
Fragments arranged by size:
5409 1442 1254 775 178
FspI TGC'GCA
Cuts at: 7564 7564
Size: 9058
HaeII r_GCGC'y
Cuts at: 1678 2443 8205 1678
Size: 765 5762 2531
Fragments arranged by size:
5762 2531 765
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2225 2257 2656 2688 3576
Size: 32 135 32 653 32 399 32 888
Cuts at: 3576 3608 3721 3753 3766 3798 4028 4060 4295
Size: 32 113 32 13 32 230 32 235
Cuts at: 4295 4327 7699 7731 7773 7805 1373
Size: 32 3372 32 42 32 2626
Fragments arranged by size:
3372 2626 888 653 399 235 230 135
113 42 32 32 32 32 32 32
32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2161 3689 4128 6402 6648 669
Size: 988 281 223 1528 439 2274 246 3079
Fragments arranged by size:
3079 2274 1528 988 439 281 246 223
HindIII A'AGCT_T
Cuts at: 748 5065 5451 748
Size: 4317 386 4355
Fragments arranged by size:
4355 4317 386
HpaI GTT'AAC
Cuts at: 2161 6648 2161
Size: 4487 4571
KpnI G_GTAC'C
Cuts at: 2055 6383 2055
Size: 4328 4730
MfeI C'AATT_G
Cuts at: 6657 6657
Size: 9058
MluI A'CGCG_T
Cuts at: 8934 8934
Size: 9058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3162 8172 8356 1305
Size: 1857 5010 184 2007
Fragments arranged by size:
5010 2007 1857 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3767 10
Size: 54 1425 343 1935 5301
Fragments arranged by size:
5301 1935 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2091 2762 3038 3600 4233 5150 5847
Size: 648 925 671 276 562 633 917 697
Cuts at: 5847 7016 7375 7534 518
Size: 1169 359 159 2042
Fragments arranged by size:
2042 1169 925 917 697 671 648 633
562 359 276 159
MspA1I CmG'CkG
Cuts at: 1606 2259 2515 2748 4686 5531 6474 7151 8299
Size: 653 256 233 1938 845 943 677 1148
Cuts at: 8299 8544 1606
Size: 245 2120
Fragments arranged by size:
2120 1938 1148 943 845 677 653 256
245 233
NcoI C'CATG_G
Cuts at: 513 1065 3981 513
Size: 552 2916 5590
Fragments arranged by size:
5590 2916 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9058
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9058
NotI GC'GGCC_GC
Cuts at: 6505 6505
Size: 9058
NspI r_CATG'y
Cuts at: 1132 1229 2458 3181 4162 4312 5775 6416 7961
Size: 97 1229 723 981 150 1463 641 1545
Cuts at: 7961 1132
Size: 2229
Fragments arranged by size:
2229 1545 1463 1229 981 723 641 150
97
PasI CC'CwG_GG
Cuts at: 1348 5513 1348
Size: 4165 4893
PciI A'CATG_T
Cuts at: 3177 4158 7957 3177
Size: 981 3799 4278
Fragments arranged by size:
4278 3799 981
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2425 1108
Size: 1317 7741
PfoI T'CCnGG_A
Cuts at: 3922 6320 3922
Size: 2398 6660
PmeI GTTT'AAAC
Cuts at: 6362 6362
Size: 9058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5237 5269 7127 7159 8640 8672 5237
Size: 32 1858 32 1481 32 5623
Fragments arranged by size:
5623 1858 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2938 3211 4667 6091 2938
Size: 273 1456 1424 5905
Fragments arranged by size:
5905 1456 1424 273
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9058
PsiI TTA'TAA
Cuts at: 5642 6628 5642
Size: 986 8072
PspOMI G'GGCC_C
Cuts at: 4369 4369
Size: 9058
PspXI vC'TCGA_Gb
Cuts at: 3219 5179 3219
Size: 1960 7098
PstI C_TGCA'G
Cuts at: 830 2837 3860 4914 6111 6410 830
Size: 2007 1023 1054 1197 299 3478
Fragments arranged by size:
3478 2007 1197 1054 1023 299
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9058
PvuII CAG'CTG
Cuts at: 4686 5531 4686
Size: 845 8213
SacI G_AGCT'C
Cuts at: 4046 5895 6073 4046
Size: 1849 178 7031
Fragments arranged by size:
7031 1849 178
SalI G'TCGA_C
Cuts at: 1936 6400 1936
Size: 4464 4594
SapI GCTCTTCn'nnn_
Cuts at: 3909 3984 4051 4296 3909
Size: 75 67 245 8671
Fragments arranged by size:
8671 245 75 67
SbfI CC_TGCA'GG
Cuts at: 6410 6410
Size: 9058
ScaI AGT'ACT
Cuts at: 4067 5326 7306 9018 4067
Size: 1259 1980 1712 4107
Fragments arranged by size:
4107 1980 1712 1259
SexAI A'CCwGG_T
Cuts at: 3960 3960
Size: 9058
SfcI C'TryA_G
Cuts at: 826 1045 2524 2620 2833 2909 3304 3856 4403
Size: 219 1479 96 213 76 395 552 547
Cuts at: 4403 4910 5157 6107 6406 7541 8222 8413 826
Size: 507 247 950 299 1135 681 191 1471
Fragments arranged by size:
1479 1471 1135 950 681 552 547 507
395 299 247 219 213 191 96 76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9058
SmaI CCC'GGG
Cuts at: 1872 6385 1872
Size: 4513 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2056 2132 3219 4530 5179 5232
Size: 197 926 113 76 1087 1311 649 53
Cuts at: 5232 7162 8063 8325 820
Size: 1930 901 262 1553
Fragments arranged by size:
1930 1553 1311 1087 926 901 649 262
197 113 76 53
SnaBI TAC'GTA
Cuts at: 493 6849 493
Size: 6356 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9058
SphI G_CATG'C
Cuts at: 1132 6416 1132
Size: 5284 3774
SspI AAT'ATT
Cuts at: 5 52 6982 5
Size: 47 6930 2081
Fragments arranged by size:
6930 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9058
StyI C'CwwG_G
Cuts at: 513 1065 2166 3701 3981 4539 4847 6147 6497
Size: 552 1101 1535 280 558 308 1300 350
Cuts at: 6497 513
Size: 3074
Fragments arranged by size:
3074 1535 1300 1101 558 552 350 308
280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2432 2837 2841 3495 4696 5100 5482
Size: 1255 852 405 4 654 1201 404 382
Cuts at: 5482 5813 7085 7102 7255 7440 7885 325
Size: 331 1272 17 153 185 445 1498
Fragments arranged by size:
1498 1272 1255 1201 852 654 445 405
404 382 331 185 153 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3174 3202 4065
Size: 274 80 33 51 1443 1197 28 863
Cuts at: 4065 5324 7304 9016 96
Size: 1259 1980 1712 138
Fragments arranged by size:
1980 1712 1443 1259 1197 863 274 138
80 51 33 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4828 4860 9018 9050 4828
Size: 32 4158 32 4836
Fragments arranged by size:
4836 4158 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9058
XbaI T'CTAG_A
Cuts at: 6394 6394
Size: 9058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4546 4711 4546
Size: 165 8893
XhoI C'TCGA_G
Cuts at: 1943 3219 5179 1943
Size: 1276 1960 5822
Fragments arranged by size:
5822 1960 1276
XmaI C'CCGG_G
Cuts at: 1870 6383 1870
Size: 4513 4545
XmnI GAAnn'nnTTC
Cuts at: 2709 4518 5616 7187 2709
Size: 1809 1098 1571 4580
Fragments arranged by size:
4580 1809 1571 1098
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6866 276
Size: 53 83 186 936 5332 2468
Fragments arranged by size:
5332 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI
BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI