Restriction map for
pFN21AE3459
(Circular) (Six-base) MAPSORT of: pFN21AE3459.seq Check: 3759 from: 1 to: 8731
pFN21AE3459 8731 bp
With 182 enzymes: SgfI *
February 23, 2012 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2017 2017
Size: 8731
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6541 278
Size: 53 83 186 936 5005 2468
Fragments arranged by size:
5005 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2334 2796 4824 6074 1937
Size: 397 462 2028 1250 4594
Fragments arranged by size:
4594 2028 1250 462 397
Acc65I G'GTAC_C
Cuts at: 6052 6052
Size: 8731
AclI AA'CG_TT
Cuts at: 1563 6858 7231 1563
Size: 5295 373 3063
Fragments arranged by size:
5295 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3214 3362 4779 5523 5589 6794 8178 8446
Size: 1582 148 1417 744 66 1205 1384 268
Cuts at: 8446 1632
Size: 1917
Fragments arranged by size:
1917 1582 1417 1384 1205 744 268 148
66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8534
AflIII A'CryG_T
Cuts at: 1203 7630 8607 1203
Size: 6427 977 1327
Fragments arranged by size:
6427 1327 977
AgeI A'CCGG_T
Cuts at: 7562 7562
Size: 8731
AhdI GACnn_n'nnGTC
Cuts at: 7460 7460
Size: 8731
AleI CACnn'nnGTG
Cuts at: 1166 4677 1166
Size: 3511 5220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8731
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 8699
AlwNI CAG_nnn'CTG
Cuts at: 2032 2149 2550 2916 3011 3480 3545 4435 5219
Size: 117 401 366 95 469 65 890 784
Cuts at: 5219 5315 5858 5966 6474 8046 2032
Size: 96 543 108 508 1572 2717
Fragments arranged by size:
2717 1572 890 784 543 508 469 401
366 117 108 96 95 65
ApaI G_GGCC'C
Cuts at: 2667 3539 3753 3800 2667
Size: 872 214 47 7598
Fragments arranged by size:
7598 872 214 47
ApaLI G'TGCA_C
Cuts at: 4172 6788 7944 4172
Size: 2616 1156 4959
Fragments arranged by size:
4959 2616 1156
ApoI r'AATT_y
Cuts at: 1493 2282 3629 6040 6266 6418 8527 1493
Size: 789 1347 2411 226 152 2109 1697
Fragments arranged by size:
2411 2109 1697 1347 789 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7285 160
Size: 872 6253 1606
Fragments arranged by size:
6253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8731
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 3037 3624 3706 4153 4911 5450
Size: 73 112 982 587 82 447 758 539
Cuts at: 5450 5520 6056 1870
Size: 70 536 4545
Fragments arranged by size:
4545 982 758 587 539 536 447 112
82 73 70
AvrII C'CTAG_G
Cuts at: 3784 5639 3784
Size: 1855 6876
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4910 4943 4910
Size: 33 8698
BamHI G'GATC_C
Cuts at: 4188 6061 6428 4188
Size: 1873 367 6491
Fragments arranged by size:
6491 1873 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3788 4008 5235 5528 6052
Size: 325 217 627 2001 220 1227 293 524
Cuts at: 6052 7507 618
Size: 1455 1842
Fragments arranged by size:
2001 1842 1455 1227 627 524 325 293
220 217
BanII G_rGCy'C
Cuts at: 1391 2063 2667 2955 3539 3713 3753 3800 4737
Size: 672 604 288 584 174 40 47 937
Cuts at: 4737 4980 5090 5328 5375 6050 1391
Size: 243 110 238 47 675 4072
Fragments arranged by size:
4072 937 675 672 604 584 288 243
238 174 110 47 47 40
BbeI G_GCGC'C
Cuts at: 4012 4012
Size: 8731
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2610 3154 5784 928
Size: 971 711 544 2630 3875
Fragments arranged by size:
3875 2630 971 711 544
BbvCI CC'TCA_GC
Cuts at: 5096 5096
Size: 8731
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2225 2259 6922 6956 1903
Size: 34 288 34 4663 34 3678
Fragments arranged by size:
4663 3678 288 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4661 5156 6623 7833 4661
Size: 495 1467 1210 5559
Fragments arranged by size:
5559 1467 1210 495
BclI T'GATC_A
Cuts at: 1548 3096 3297 1548
Size: 1548 201 6982
Fragments arranged by size:
6982 1548 201
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3641 5843
Size: 107 122 71 631 605 337 1632 2202
Cuts at: 5843 7342 136
Size: 1499 1525
Fragments arranged by size:
2202 1632 1525 1499 631 605 337 122
107 71
BglII A'GATC_T
Cuts at: 4806 8726 4806
Size: 3920 4811
BlpI GC'TnA_GC
Cuts at: 5322 6148 5322
Size: 826 7905
Bme1580I G_kGCm'C
Cuts at: 1790 2667 3539 3689 3736 3753 3800 4176 4420
Size: 877 872 150 47 17 47 376 244
Cuts at: 4420 5240 6792 7948 1790
Size: 820 1552 1156 2573
Fragments arranged by size:
2573 1552 1156 877 872 820 376 244
150 47 47 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4074 4430 4453
Size: 416 53 83 399 24 2657 356 23
Cuts at: 4453 4533 5093 6462 7420 442
Size: 80 560 1369 958 1753
Fragments arranged by size:
2657 1753 1369 958 560 416 399 356
83 80 53 24 23
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2368 2512 4708 5039 5312 5754 7391 1239
Size: 1129 144 2196 331 273 442 1637 2579
Fragments arranged by size:
2579 2196 1637 1129 442 331 273 144
Bpu10I CC'TnA_GC
Cuts at: 2710 4130 5096 2710
Size: 1420 966 6345
Fragments arranged by size:
6345 1420 966
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2213 2939 4499 4514 4664 5675 6856 7721 8019
Size: 726 1560 15 150 1011 1181 865 298
Cuts at: 8019 2213
Size: 2925
Fragments arranged by size:
2925 1560 1181 1011 865 726 298 150
15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3108 4908 5552 7394 882
Size: 617 1609 1800 644 1842 2219
Fragments arranged by size:
2219 1842 1800 1609 644 617
BsaAI yAC'GTr
Cuts at: 493 1204 5129 5945 6522 493
Size: 711 3925 816 577 2702
Fragments arranged by size:
3925 2702 816 711 577
BsaBI GATnn'nnATC
Cuts at: 8725 8725
Size: 8731
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4009 6538 6920 7555
Size: 53 83 186 936 2476 2529 382 635
Cuts at: 7555 275
Size: 1451
Fragments arranged by size:
2529 2476 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3165 5607 7164 7562 7836 7983 8394 3165
Size: 2442 1557 398 274 147 411 3502
Fragments arranged by size:
3502 2442 1557 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2366 2396 2754 2784 2847
Size: 30 165 30 963 30 358 30 63
Cuts at: 2847 2877 4681 4711 5324 5354 1178
Size: 30 1804 30 613 30 4555
Fragments arranged by size:
4555 1804 963 613 358 165 63 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2628 2868 3508 3594 5072 5506 5603 5626
Size: 1442 240 640 86 1478 434 97 23
Cuts at: 5626 5672 1186
Size: 46 4245
Fragments arranged by size:
4245 1478 1442 640 434 240 97 86
46 23
BseYI C'CCAG_C
Cuts at: 2032 2586 3356 3531 3979 4327 5406 5662 5851
Size: 554 770 175 448 348 1079 256 189
Cuts at: 5851 7934 8430 2032
Size: 2083 496 2333
Fragments arranged by size:
2333 2083 1079 770 554 496 448 348
256 189 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3049 3838 4795 1163
Size: 573 1313 789 957 5099
Fragments arranged by size:
5099 1313 957 789 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3470 6181 6942 7970 1540
Size: 275 186 1469 2711 761 1028 2301
Fragments arranged by size:
2711 2301 1469 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2205 2638 3518 3727 4176 5328 6792 6877 7948
Size: 433 880 209 449 1152 1464 85 1071
Cuts at: 7948 2205
Size: 2988
Fragments arranged by size:
2988 1464 1152 1071 880 449 433 209
85
BsmI GAATG_Cn'
Cuts at: 3653 3674 6242 6335 3653
Size: 21 2568 93 6049
Fragments arranged by size:
6049 2568 93 21
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2205 2638 2667 2955 3518 3539
Size: 399 273 142 433 29 288 563 21
Cuts at: 3539 3689 3713 3727 3736 3753 3800 4176 4420
Size: 150 24 14 9 17 47 376 244
Cuts at: 4420 4737 4980 5090 5240 5328 5375 6050 6792
Size: 317 243 110 150 88 47 675 742
Cuts at: 6792 6877 7948 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 675 563 433 399 376
317 288 273 244 243 150 150 142
110 88 85 47 47 29 24 21
17 14 9
BspEI T'CCGG_A
Cuts at: 3165 8394 3165
Size: 5229 3502
BspHI T'CATG_A
Cuts at: 2563 3300 3972 6620 8350 2563
Size: 737 672 2648 1730 2944
Fragments arranged by size:
2944 2648 1730 737 672
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2017 4584 4779 6086 844
Size: 1173 2567 195 1307 3489
Fragments arranged by size:
3489 2567 1307 1173 195
BsrBI CCG'CTC
Cuts at: 1394 1823 4789 6618 1394
Size: 429 2966 1829 3507
Fragments arranged by size:
3507 2966 1829 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2297 3706 7226 7400 65
Size: 1183 1049 1409 3520 174 1396
Fragments arranged by size:
3520 1409 1396 1183 1049 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2120 2742 3101 7375 7562 1700
Size: 110 310 622 359 4274 187 2869
Fragments arranged by size:
4274 2869 622 359 310 187 110
BsrGI T'GTAC_A
Cuts at: 96 2151 96
Size: 2055 6676
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8731
BssSI C'ACGA_G
Cuts at: 2267 6791 7803 2267
Size: 4524 1012 3195
Fragments arranged by size:
4524 3195 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3480 794
Size: 2686 6045
BstBI TT'CG_AA
Cuts at: 7535 7535
Size: 8731
BstEII G'GTnAC_C
Cuts at: 1184 3188 1184
Size: 2004 6727
BstXI CCAn_nnnn'nTGG
Cuts at: 3076 4632 5083 3076
Size: 1556 451 6724
Fragments arranged by size:
6724 1556 451
BstYI r'GATC_y
Cuts at: 1972 3072 3129 4188 4806 6061 6428 6813 6830
Size: 1100 57 1059 618 1255 367 385 17
Cuts at: 6830 8282 8368 8380 8726 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1255 1100 1059 618 385 367
346 86 57 17 12
Bsu36I CC'TnA_GG
Cuts at: 2718 4194 2718
Size: 1476 7255
BtgI C'CryG_G
Cuts at: 513 1065 3678 3775 4922 4981 5267 5375 5838
Size: 552 2613 97 1147 59 286 108 463
Cuts at: 5838 513
Size: 3406
Fragments arranged by size:
3406 2613 1147 552 463 286 108 97
59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2139 2596 487
Size: 683 56 913 457 6622
Fragments arranged by size:
6622 913 683 457 56
BtsI GCAGTG_nn'
Cuts at: 2091 2243 2653 3598 3687 5233 5754 6247 7041
Size: 152 410 945 89 1546 521 493 794
Cuts at: 7041 7061 2091
Size: 20 3761
Fragments arranged by size:
3761 1546 945 794 521 493 410 152
89 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2740 2775 529
Size: 35 2176 35 6485
Fragments arranged by size:
6485 2176 35 35
DraI TTT'AAA
Cuts at: 4879 6035 4879
Size: 1156 7575
DraIII CAC_nnn'GTG
Cuts at: 3760 3760
Size: 8731
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7738 809
Size: 507 6422 1802
Fragments arranged by size:
6422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2271 3209 3467
Size: 54 1425 50 275 18 441 938 258
Cuts at: 3467 4104 4287 5123 5378 6178 7067 8696 8
Size: 637 183 836 255 800 889 1629 43
Fragments arranged by size:
1629 1425 938 889 836 800 637 441
275 258 255 183 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3467 6178 1537
Size: 275 1655 2711 4090
Fragments arranged by size:
4090 2711 1655 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3435 5457 5868 6661 1353
Size: 2082 2022 411 793 3423
Fragments arranged by size:
3423 2082 2022 793 411
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5080 7318 7692 7838 387
Size: 1064 138 3491 2238 374 146 1280
Fragments arranged by size:
3491 2238 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5326 5326
Size: 8731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2368 2512 3214 3362 4708 4779 5039
Size: 393 736 144 702 148 1346 71 260
Cuts at: 5039 5312 5523 5589 5754 6794 7391 8178 8446
Size: 273 211 66 165 1040 597 787 268
Cuts at: 8446 1239
Size: 1524
Fragments arranged by size:
1524 1346 1040 787 736 702 597 393
273 268 260 211 165 148 144 71
66
EcoNI CCTnn'n_nnAGG
Cuts at: 2908 2908
Size: 8731
EcoO109I rG'GnC_Cy
Cuts at: 2325 2591 2714 2913 3431 3535 3542 3750 3796
Size: 266 123 199 518 104 7 208 46
Cuts at: 3796 5844 5894 5908 2325
Size: 2048 50 14 5148
Fragments arranged by size:
5148 2048 518 266 208 199 123 104
50 46 14 7
EcoRI G'AATT_C
Cuts at: 2282 6040 6418 2282
Size: 3758 378 4595
Fragments arranged by size:
4595 3758 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5549 5549
Size: 8731
FspI TGC'GCA
Cuts at: 7237 7237
Size: 8731
HaeII r_GCGC'y
Cuts at: 1678 4012 5397 5413 7878 1678
Size: 2334 1385 16 2465 2531
Fragments arranged by size:
2531 2465 2334 1385 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2417 2449 2885 2917 3438
Size: 32 135 32 845 32 436 32 521
Cuts at: 3438 3470 3570 3602 4173 4187 4205 4219 5324
Size: 32 100 32 571 14 18 14 1105
Cuts at: 5324 5356 5672 5704 7372 7404 7446 7478 1373
Size: 32 316 32 1668 32 42 32 2626
Fragments arranged by size:
2626 1668 1105 845 571 521 436 316
135 100 42 32 32 32 32 32
32 32 32 32 32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 5473 6075 6321 669
Size: 988 281 3535 602 246 3079
Fragments arranged by size:
3535 3079 988 602 281 246
HindIII A'AGCT_T
Cuts at: 748 2465 4136 748
Size: 1717 1671 5343
Fragments arranged by size:
5343 1717 1671
HpaI GTT'AAC
Cuts at: 6321 6321
Size: 8731
KasI G'GCGC_C
Cuts at: 4008 4008
Size: 8731
KpnI G_GTAC'C
Cuts at: 6056 6056
Size: 8731
MfeI C'AATT_G
Cuts at: 2484 4815 6330 2484
Size: 2331 1515 4885
Fragments arranged by size:
4885 2331 1515
MluI A'CGCG_T
Cuts at: 8607 8607
Size: 8731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4826 5089 7845 8029 1305
Size: 3521 263 2756 184 2007
Fragments arranged by size:
3521 2756 2007 263 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2273 4106 4289 10
Size: 54 1425 343 441 1833 183 4452
Fragments arranged by size:
4452 1833 1425 441 343 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2751 4677 4832 4904 5171 5272 5681
Size: 648 1585 1926 155 72 267 101 409
Cuts at: 5681 6689 7048 7207 518
Size: 1008 359 159 2042
Fragments arranged by size:
2042 1926 1585 1008 648 409 359 267
159 155 101 72
MspA1I CmG'CkG
Cuts at: 1606 2818 2871 3768 4432 4600 6147 6824 7972
Size: 1212 53 897 664 168 1547 677 1148
Cuts at: 7972 8217 1606
Size: 245 2120
Fragments arranged by size:
2120 1547 1212 1148 897 677 664 245
168 53
NaeI GCC'GGC
Cuts at: 2744 2744
Size: 8731
NarI GG'CG_CC
Cuts at: 4009 4009
Size: 8731
NcoI C'CATG_G
Cuts at: 513 1065 3678 3775 4981 5838 513
Size: 552 2613 97 1206 857 3406
Fragments arranged by size:
3406 2613 1206 857 552 97
NdeI CA'TA_TG
Cuts at: 387 4281 387
Size: 3894 4837
NgoMIV G'CCGG_C
Cuts at: 2742 2742
Size: 8731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8731
NotI GC'GGCC_GC
Cuts at: 6178 6178
Size: 8731
NspI r_CATG'y
Cuts at: 1132 1229 3223 3699 5170 5306 6089 7634 1132
Size: 97 1994 476 1471 136 783 1545 2229
Fragments arranged by size:
2229 1994 1545 1471 783 476 136 97
PasI CC'CwG_GG
Cuts at: 1348 3859 4340 4967 1348
Size: 2511 481 627 5112
Fragments arranged by size:
5112 2511 627 481
PciI A'CATG_T
Cuts at: 7630 7630
Size: 8731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3031 3545 3679 5159 1108
Size: 1923 514 134 1480 4680
Fragments arranged by size:
4680 1923 1480 514 134
PfoI T'CCnGG_A
Cuts at: 2593 5328 2593
Size: 2735 5996
PmeI GTTT'AAAC
Cuts at: 6035 6035
Size: 8731
PmlI CAC'GTG
Cuts at: 5129 5129
Size: 8731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6800 6832 8313 8345 6800
Size: 32 1481 32 7186
Fragments arranged by size:
7186 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2325 2591 2325
Size: 266 8465
PshAI GACnn'nnGTC
Cuts at: 1613 2330 1613
Size: 717 8014
PsiI TTA'TAA
Cuts at: 6301 6301
Size: 8731
PspOMI G'GGCC_C
Cuts at: 2663 3535 3749 3796 2663
Size: 872 214 47 7598
Fragments arranged by size:
7598 872 214 47
PspXI vC'TCGA_Gb
Cuts at: 3037 3037
Size: 8731
PstI C_TGCA'G
Cuts at: 830 2028 2238 4356 5215 5761 6083 830
Size: 1198 210 2118 859 546 322 3478
Fragments arranged by size:
3478 2118 1198 859 546 322 210
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8731
PvuII CAG'CTG
Cuts at: 2818 2871 3768 4432 4600 2818
Size: 53 897 664 168 6949
Fragments arranged by size:
6949 897 664 168 53
SacI G_AGCT'C
Cuts at: 5328 5328
Size: 8731
SalI G'TCGA_C
Cuts at: 1936 6073 1936
Size: 4137 4594
SanDI GG'GwC_CC
Cuts at: 2325 2325
Size: 8731
SapI GCTCTTCn'nnn_
Cuts at: 3435 3435
Size: 8731
SbfI CC_TGCA'GG
Cuts at: 6083 6083
Size: 8731
ScaI AGT'ACT
Cuts at: 6979 8691 6979
Size: 1712 7019
SfcI C'TryA_G
Cuts at: 826 1045 2024 2234 4352 5211 5757 5958 6079
Size: 219 979 210 2118 859 546 201 121
Cuts at: 6079 7214 7895 8086 826
Size: 1135 681 191 1471
Fragments arranged by size:
2118 1471 1135 979 859 681 546 219
210 201 191 121
SfoI GGC'GCC
Cuts at: 4010 4010
Size: 8731
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8731
SmaI CCC'GGG
Cuts at: 1872 2057 5452 5522 6058 1872
Size: 185 3395 70 536 4545
Fragments arranged by size:
4545 3395 536 185 70
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2192 2954 3037 3624 4478 4529
Size: 197 926 249 762 83 587 854 51
Cuts at: 4529 4643 5654 6835 7736 7998 820
Size: 114 1011 1181 901 262 1553
Fragments arranged by size:
1553 1181 1011 926 901 854 762 587
262 249 197 114 83 51
SnaBI TAC'GTA
Cuts at: 493 5945 6522 493
Size: 5452 577 2702
Fragments arranged by size:
5452 2702 577
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8731
SphI G_CATG'C
Cuts at: 1132 6089 1132
Size: 4957 3774
SspI AAT'ATT
Cuts at: 5 52 4370 6655 5
Size: 47 4318 2285 2081
Fragments arranged by size:
4318 2285 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8731
StyI C'CwwG_G
Cuts at: 513 1065 3417 3678 3775 3784 3792 4461 4981
Size: 552 2352 261 97 9 8 669 520
Cuts at: 4981 5639 5838 6009 6170 513
Size: 658 199 171 161 3074
Fragments arranged by size:
3074 2352 669 658 552 520 261 199
171 161 97 9 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2526 3872 4552 5274 6758 6775 6928
Size: 1255 946 1346 680 722 1484 17 153
Cuts at: 6928 7113 7558 325
Size: 185 445 1498
Fragments arranged by size:
1498 1484 1346 1255 946 722 680 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2151 4450 4470
Size: 274 80 33 51 1443 174 2299 20
Cuts at: 4470 5673 6977 8689 96
Size: 1203 1304 1712 138
Fragments arranged by size:
2299 1712 1443 1304 1203 274 174 138
80 51 33 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3948 3980 4681 4713 8691 8723 3948
Size: 32 701 32 3978 32 3956
Fragments arranged by size:
3978 3956 701 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2982 1316
Size: 1666 7065
XbaI T'CTAG_A
Cuts at: 6067 6067
Size: 8731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 2336 3743 4297 5186 2311
Size: 25 1407 554 889 5856
Fragments arranged by size:
5856 1407 889 554 25
XhoI C'TCGA_G
Cuts at: 1943 3037 3624 1943
Size: 1094 587 7050
Fragments arranged by size:
7050 1094 587
XmaI C'CCGG_G
Cuts at: 1870 2055 5450 5520 6056 1870
Size: 185 3395 70 536 4545
Fragments arranged by size:
4545 3395 536 185 70
XmnI GAAnn'nnTTC
Cuts at: 2282 6860 2282
Size: 4578 4153
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6539 276
Size: 53 83 186 936 5005 2468
Fragments arranged by size:
5005 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV
FseI FspAI NruI NsiI PacI PsrI RsrII SacII
SexAI SfiI SrfI SwaI