Restriction map for
pFN21AE3457
(Circular) (Six-base) MAPSORT of: pFN21AE3457.seq Check: 814 from: 1 to: 8728
pFN21AE3457 8728 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2139 2529 6538 278
Size: 53 83 186 936 603 390 4009 2468
Fragments arranged by size:
4009 2468 936 603 390 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2115 3328 6071 1937
Size: 178 1213 2743 4594
Fragments arranged by size:
4594 2743 1213 178
Acc65I G'GTAC_C
Cuts at: 6049 6049
Size: 8728
AclI AA'CG_TT
Cuts at: 1563 6855 7228 1563
Size: 5292 373 3063
Fragments arranged by size:
5292 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2495 3680 4037 4394 4551 5258 5619 6791
Size: 863 1185 357 357 157 707 361 1172
Cuts at: 6791 8175 8443 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1185 1172 863 707 361 357
357 268 157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8531
AflIII A'CryG_T
Cuts at: 1203 5736 7627 8604 1203
Size: 4533 1891 977 1327
Fragments arranged by size:
4533 1891 1327 977
AgeI A'CCGG_T
Cuts at: 7559 7559
Size: 8728
AhdI GACnn_n'nnGTC
Cuts at: 7457 7457
Size: 8728
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8728
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5487 5519 5487
Size: 32 8696
AlwNI CAG_nnn'CTG
Cuts at: 6471 8043 6471
Size: 1572 7156
ApaLI G'TGCA_C
Cuts at: 2336 6785 7941 2336
Size: 4449 1156 3123
Fragments arranged by size:
4449 3123 1156
ApoI r'AATT_y
Cuts at: 1493 2402 2850 2948 3050 3526 3883 4597 6037
Size: 909 448 98 102 476 357 714 1440
Cuts at: 6037 6263 6415 8524 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1440 909 714 476 448 357
226 152 102 98
AseI AT'TA_AT
Cuts at: 160 1032 5631 7282 160
Size: 872 4599 1651 1606
Fragments arranged by size:
4599 1651 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8728
AvaI C'yCGr_G
Cuts at: 1870 1943 4100 4457 6053 1870
Size: 73 2157 357 1596 4545
Fragments arranged by size:
4545 2157 1596 357 73
AvrII C'CTAG_G
Cuts at: 2909 2975 3011 3077 2909
Size: 66 36 66 8560
Fragments arranged by size:
8560 66 66 36
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5723 5756 5723
Size: 33 8695
BamHI G'GATC_C
Cuts at: 6058 6425 6058
Size: 367 8361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2222 6049 7504 618
Size: 325 217 627 435 3827 1455 1842
Fragments arranged by size:
3827 1842 1455 627 435 325 217
BanII G_rGCy'C
Cuts at: 1391 4908 6047 1391
Size: 3517 1139 4072
Fragments arranged by size:
4072 3517 1139
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2027 2977 3009 3079 3647 4004 928
Size: 971 128 950 32 70 568 357 5652
Fragments arranged by size:
5652 971 950 568 357 128 70 32
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6919 6953 1903
Size: 34 4982 34 3678
Fragments arranged by size:
4982 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3886 4726 6620 7830 3886
Size: 840 1894 1210 4784
Fragments arranged by size:
4784 1894 1210 840
BclI T'GATC_A
Cuts at: 1548 3229 5464 1548
Size: 1681 2235 4812
Fragments arranged by size:
4812 2235 1681
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7339 136
Size: 107 122 71 631 605 5667 1525
Fragments arranged by size:
5667 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3173 8723 3173
Size: 5550 3178
BlpI GC'TnA_GC
Cuts at: 6145 6145
Size: 8728
Bme1580I G_kGCm'C
Cuts at: 1790 2340 6789 7945 1790
Size: 550 4449 1156 2573
Fragments arranged by size:
4449 2573 1156 550
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8728
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2080 6459 7417
Size: 416 53 83 399 24 663 4379 958
Cuts at: 7417 442
Size: 1753
Fragments arranged by size:
4379 1753 958 663 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2352 1056
Size: 1296 7432
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2079 2079
Size: 8728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 7388 1239
Size: 6149 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5927 6853 7718 8016 5927
Size: 926 865 298 6639
Fragments arranged by size:
6639 926 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3999 5688 7391 882
Size: 617 2500 1689 1703 2219
Fragments arranged by size:
2500 2219 1703 1689 617
BsaAI yAC'GTr
Cuts at: 493 1204 2955 3057 6519 493
Size: 711 1751 102 3462 2702
Fragments arranged by size:
3462 2702 1751 711 102
BsaBI GATnn'nnATC
Cuts at: 2232 8722 2232
Size: 6490 2238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 2136 2526 6535
Size: 53 83 186 936 513 90 390 4009
Cuts at: 6535 6917 7552 275
Size: 382 635 1451
Fragments arranged by size:
4009 1451 936 635 513 390 382 186
90 83 53
BsaWI w'CCGG_w
Cuts at: 7161 7559 7833 7980 8391 7161
Size: 398 274 147 411 7498
Fragments arranged by size:
7498 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5913 5943 1178
Size: 30 165 30 4510 30 3963
Fragments arranged by size:
4510 3963 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5963 1186
Size: 4777 3951
BseYI C'CCAG_C
Cuts at: 7931 8427 7931
Size: 496 8232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2343 2673 2739 1163
Size: 573 607 330 66 7152
Fragments arranged by size:
7152 607 573 330 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6178 6939 7967 1540
Size: 275 186 4177 761 1028 2301
Fragments arranged by size:
4177 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2340 4908 5583 6789 6874 7945 2340
Size: 2568 675 1206 85 1071 3123
Fragments arranged by size:
3123 2568 1206 1071 675 85
BsmI GAATG_Cn'
Cuts at: 2437 5269 6239 6332 2437
Size: 2832 970 93 4833
Fragments arranged by size:
4833 2832 970 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2340 4908 5583 6047 6789 6874 7945
Size: 399 550 2568 675 464 742 85 1071
Cuts at: 7945 1391
Size: 2174
Fragments arranged by size:
2568 2174 1071 742 675 550 464 399
85
BspEI T'CCGG_A
Cuts at: 8391 8391
Size: 8728
BspHI T'CATG_A
Cuts at: 2596 5116 6617 8347 2596
Size: 2520 1501 1730 2977
Fragments arranged by size:
2977 2520 1730 1501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3677 5506 6083 844
Size: 2833 1829 577 3489
Fragments arranged by size:
3489 2833 1829 577
BsrBI CCG'CTC
Cuts at: 1394 1823 6615 1394
Size: 429 4792 3507
Fragments arranged by size:
4792 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5508 7223 7397 65
Size: 1183 4260 1715 174 1396
Fragments arranged by size:
4260 1715 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7372 7559 1700
Size: 110 5562 187 2869
Fragments arranged by size:
5562 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5452 96
Size: 5356 3372
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8728
BssSI C'ACGA_G
Cuts at: 6788 7800 6788
Size: 1012 7716
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5519 5651 794
Size: 4725 132 3871
Fragments arranged by size:
4725 3871 132
BstBI TT'CG_AA
Cuts at: 2886 7532 2886
Size: 4646 4082
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8728
BstYI r'GATC_y
Cuts at: 1972 3173 3481 6058 6425 6810 6827 8279 8365
Size: 1201 308 2577 367 385 17 1452 86
Cuts at: 8365 8377 8723 1972
Size: 12 346 1977
Fragments arranged by size:
2577 1977 1452 1201 385 367 346 308
86 17 12
BstZ17I GTA'TAC
Cuts at: 3329 3329
Size: 8728
BtgI C'CryG_G
Cuts at: 513 1065 2317 513
Size: 552 1252 6924
Fragments arranged by size:
6924 1252 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7989
Fragments arranged by size:
7989 683 56
BtsI GCAGTG_nn'
Cuts at: 4918 6244 7038 7058 4918
Size: 1326 794 20 6588
Fragments arranged by size:
6588 1326 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8693
DraI TTT'AAA
Cuts at: 4929 5253 5976 6032 4929
Size: 324 723 56 7625
Fragments arranged by size:
7625 723 324 56
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7735 809
Size: 507 6419 1802
Fragments arranged by size:
6419 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2086 2957 6175
Size: 54 1425 50 275 18 256 871 3218
Cuts at: 6175 7064 8693 8
Size: 889 1629 43
Fragments arranged by size:
3218 1629 1425 889 871 275 256 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6175 1537
Size: 275 4363 4090
Fragments arranged by size:
4363 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2048 3372 3627 4341 4935 4940 4998 5072
Size: 695 1324 255 714 594 5 58 74
Cuts at: 5072 6658 1353
Size: 1586 3423
Fragments arranged by size:
3423 1586 1324 714 695 594 255 74
58 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2714 7315 7689 7835 387
Size: 1064 138 1125 4601 374 146 1280
Fragments arranged by size:
4601 1280 1125 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4906 4906
Size: 8728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2495 3680 4037 4394 4551 5258 5619
Size: 393 863 1185 357 357 157 707 361
Cuts at: 5619 6791 7388 8175 8443 1239
Size: 1172 597 787 268 1524
Fragments arranged by size:
1524 1185 1172 863 787 707 597 393
361 357 357 268 157
EcoRI G'AATT_C
Cuts at: 6037 6415 6037
Size: 378 8350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3671 4028 3671
Size: 357 8371
FspI TGC'GCA
Cuts at: 3951 7234 3951
Size: 3283 5445
HaeII r_GCGC'y
Cuts at: 1678 7875 1678
Size: 6197 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2158 2190 2276 2308 2807
Size: 32 135 32 586 32 86 32 499
Cuts at: 2807 2825 2839 2857 3175 3207 3466 3498 4094
Size: 18 14 18 318 32 259 32 596
Cuts at: 4094 4126 4451 4483 4537 4569 7369 7401 7443
Size: 32 325 32 54 32 2800 32 42
Cuts at: 7443 7475 1373
Size: 32 2626
Fragments arranged by size:
2800 2626 596 586 499 325 318 259
135 86 54 42 32 32 32 32
32 32 32 32 32 32 32 18
18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2524 6072 6318 669
Size: 988 281 586 3548 246 3079
Fragments arranged by size:
3548 3079 988 586 281 246
HindIII A'AGCT_T
Cuts at: 748 2725 2791 748
Size: 1977 66 6685
Fragments arranged by size:
6685 1977 66
HpaI GTT'AAC
Cuts at: 6318 6318
Size: 8728
KpnI G_GTAC'C
Cuts at: 6053 6053
Size: 8728
MfeI C'AATT_G
Cuts at: 5889 6327 5889
Size: 438 8290
MluI A'CGCG_T
Cuts at: 8604 8604
Size: 8728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3716 4073 4430 7842 8026 1305
Size: 2411 357 357 3412 184 2007
Fragments arranged by size:
3412 2411 2007 357 357 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2088 2959 10
Size: 54 1425 343 256 871 5779
Fragments arranged by size:
5779 1425 871 343 256 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2333 2595 3419 3776 4133 4490 4746
Size: 648 1167 262 824 357 357 357 256
Cuts at: 4746 5115 5121 6686 7045 7204 518
Size: 369 6 1565 359 159 2042
Fragments arranged by size:
2042 1565 1167 824 648 369 359 357
357 357 262 256 159 6
MspA1I CmG'CkG
Cuts at: 1606 2720 6144 6821 7969 8214 1606
Size: 1114 3424 677 1148 245 2120
Fragments arranged by size:
3424 2120 1148 1114 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2317 513
Size: 552 1252 6924
Fragments arranged by size:
6924 1252 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8728
NheI G'CTAG_C
Cuts at: 1052 2348 1052
Size: 1296 7432
NotI GC'GGCC_GC
Cuts at: 6175 6175
Size: 8728
NspI r_CATG'y
Cuts at: 1132 1229 2506 3192 3721 4078 4435 5155 5687
Size: 97 1277 686 529 357 357 720 532
Cuts at: 5687 5740 6086 7631 1132
Size: 53 346 1545 2229
Fragments arranged by size:
2229 1545 1277 720 686 532 529 357
357 346 97 53
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8728
PciI A'CATG_T
Cuts at: 5736 7627 5736
Size: 1891 6837
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8728
PmeI GTTT'AAAC
Cuts at: 6032 6032
Size: 8728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4890 4922 5037 5069 6797 6829 8310 8342 4890
Size: 32 115 32 1728 32 1481 32 5276
Fragments arranged by size:
5276 1728 1481 115 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8728
PsiI TTA'TAA
Cuts at: 6298 6298
Size: 8728
PstI C_TGCA'G
Cuts at: 830 2539 2560 2671 4388 4913 6080 830
Size: 1709 21 111 1717 525 1167 3478
Fragments arranged by size:
3478 1717 1709 1167 525 111 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8728
PvuII CAG'CTG
Cuts at: 2720 2720
Size: 8728
SacI G_AGCT'C
Cuts at: 4908 4908
Size: 8728
SalI G'TCGA_C
Cuts at: 1936 6070 1936
Size: 4134 4594
SapI GCTCTTCn'nnn_
Cuts at: 3372 4998 3372
Size: 1626 7102
SbfI CC_TGCA'GG
Cuts at: 6080 6080
Size: 8728
ScaI AGT'ACT
Cuts at: 6976 8688 6976
Size: 1712 7016
SfcI C'TryA_G
Cuts at: 826 1045 2535 2556 2667 4384 4909 6076 7211
Size: 219 1490 21 111 1717 525 1167 1135
Cuts at: 7211 7892 8083 826
Size: 681 191 1471
Fragments arranged by size:
1717 1490 1471 1167 1135 681 525 219
191 111 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8728
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8728
SmaI CCC'GGG
Cuts at: 1872 6055 1872
Size: 4183 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5906 6832 7733 7995 820
Size: 197 926 3963 926 901 262 1553
Fragments arranged by size:
3963 1553 926 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6519 493
Size: 6026 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8728
SphI G_CATG'C
Cuts at: 1132 2506 6086 1132
Size: 1374 3580 3774
Fragments arranged by size:
3774 3580 1374
SspI AAT'ATT
Cuts at: 5 52 2206 3275 6652 5
Size: 47 2154 1069 3377 2081
Fragments arranged by size:
3377 2154 2081 1069 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8728
StyI C'CwwG_G
Cuts at: 513 1065 2317 2694 2760 2826 2909 2975 3011
Size: 552 1252 377 66 66 83 66 36
Cuts at: 3011 3077 3585 3804 3942 4161 4299 4518 4656
Size: 66 508 219 138 219 138 219 138
Cuts at: 4656 6167 513
Size: 1511 3074
Fragments arranged by size:
3074 1511 1252 552 508 377 219 219
219 138 138 138 83 66 66 66
66 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6755 6772 6925 7110 7555 325
Size: 1255 5175 17 153 185 445 1498
Fragments arranged by size:
5175 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4828 5452 6974
Size: 274 80 33 51 1443 2851 624 1522
Cuts at: 6974 8686 96
Size: 1712 138
Fragments arranged by size:
2851 1712 1522 1443 624 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8688 8720 8688
Size: 32 8696
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8728
XbaI T'CTAG_A
Cuts at: 5791 6064 5791
Size: 273 8455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5179 5179
Size: 8728
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8728
XmaI C'CCGG_G
Cuts at: 1870 6053 1870
Size: 4183 4545
XmnI GAAnn'nnTTC
Cuts at: 6857 6857
Size: 8728
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2137 2527 6536 276
Size: 53 83 186 936 603 390 4009 2468
Fragments arranged by size:
4009 2468 936 603 390 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI