Restriction map for
pFN21AE3451
(Circular) (Six-base) MAPSORT of: pFN21AE3451.seq Check: 6945 from: 1 to: 8668
pFN21AE3451 8668 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3893 6478 278
Size: 53 83 186 936 2357 2585 2468
Fragments arranged by size:
2585 2468 2357 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6011 1937
Size: 4074 4594
Acc65I G'GTAC_C
Cuts at: 5989 5989
Size: 8668
AclI AA'CG_TT
Cuts at: 1563 6795 7168 1563
Size: 5232 373 3063
Fragments arranged by size:
5232 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2807 3216 6731 8115 8383 1632
Size: 1175 409 3515 1384 268 1917
Fragments arranged by size:
3515 1917 1384 1175 409 268
AfeI AGC'GCT
Cuts at: 4870 4870
Size: 8668
AflII C'TTAA_G
Cuts at: 820 1017 2027 820
Size: 197 1010 7461
Fragments arranged by size:
7461 1010 197
AflIII A'CryG_T
Cuts at: 1203 3543 7567 8544 1203
Size: 2340 4024 977 1327
Fragments arranged by size:
4024 2340 1327 977
AgeI A'CCGG_T
Cuts at: 7499 7499
Size: 8668
AhdI GACnn_n'nnGTC
Cuts at: 2671 7397 2671
Size: 4726 3942
AleI CACnn'nnGTG
Cuts at: 1166 3096 1166
Size: 1930 6738
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2414 5376 5516 1184
Size: 1230 2962 140 4336
Fragments arranged by size:
4336 2962 1230 140
AlwNI CAG_nnn'CTG
Cuts at: 3044 3493 3554 3680 3811 3850 5269 5764 6411
Size: 449 61 126 131 39 1419 495 647
Cuts at: 6411 7983 3044
Size: 1572 3729
Fragments arranged by size:
3729 1572 1419 647 495 449 131 126
61 39
ApaI G_GGCC'C
Cuts at: 3155 4930 5008 5657 5848 3155
Size: 1775 78 649 191 5975
Fragments arranged by size:
5975 1775 649 191 78
ApaLI G'TGCA_C
Cuts at: 4796 6725 7881 4796
Size: 1929 1156 5583
Fragments arranged by size:
5583 1929 1156
ApoI r'AATT_y
Cuts at: 1493 5977 6203 6355 8464 1493
Size: 4484 226 152 2109 1697
Fragments arranged by size:
4484 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7222 160
Size: 872 6190 1606
Fragments arranged by size:
6190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8668
AvaI C'yCGr_G
Cuts at: 1870 1943 3128 3179 3716 3737 3789 3937 4696
Size: 73 1185 51 537 21 52 148 759
Cuts at: 4696 4929 5010 5807 5823 5841 5870 5993 1870
Size: 233 81 797 16 18 29 123 4545
Fragments arranged by size:
4545 1185 797 759 537 233 148 123
81 73 52 51 29 21 18 16
BamHI G'GATC_C
Cuts at: 5998 6365 5998
Size: 367 8301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2375 2945 3592 3698 4003
Size: 325 217 627 588 570 647 106 305
Cuts at: 4003 4412 4583 4658 5858 5989 7444 618
Size: 409 171 75 1200 131 1455 1842
Fragments arranged by size:
1842 1455 1200 647 627 588 570 409
325 305 217 171 131 106 75
BanII G_rGCy'C
Cuts at: 1391 3155 3660 3687 3717 3777 4502 4689 4930
Size: 1764 505 27 30 60 725 187 241
Cuts at: 4930 4991 5008 5204 5211 5254 5390 5657 5668
Size: 61 17 196 7 43 136 267 11
Cuts at: 5668 5783 5848 5879 5987 1391
Size: 115 65 31 108 4072
Fragments arranged by size:
4072 1764 725 505 267 241 196 187
136 115 108 65 61 60 43 31
30 27 17 11 7
BbeI G_GCGC'C
Cuts at: 4007 4007
Size: 8668
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4824 928
Size: 971 2925 4772
Fragments arranged by size:
4772 2925 971
BbvCI CC'TCA_GC
Cuts at: 3603 4605 3603
Size: 1002 7666
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6859 6893 1903
Size: 34 50 34 4838 34 3678
Fragments arranged by size:
4838 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5180 6560 7770 5180
Size: 1380 1210 6078
Fragments arranged by size:
6078 1380 1210
BclI T'GATC_A
Cuts at: 1548 2754 2868 1548
Size: 1206 114 7348
Fragments arranged by size:
7348 1206 114
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3133 4469 4562
Size: 107 122 71 631 605 1461 1336 93
Cuts at: 4562 4699 5327 5484 7279 136
Size: 137 628 157 1795 1525
Fragments arranged by size:
1795 1525 1461 1336 631 628 605 157
137 122 107 93 71
BglII A'GATC_T
Cuts at: 2145 2415 8663 2145
Size: 270 6248 2150
Fragments arranged by size:
6248 2150 270
BlpI GC'TnA_GC
Cuts at: 2387 3681 3863 5910 6085 2387
Size: 1294 182 2047 175 4970
Fragments arranged by size:
4970 2047 1294 182 175
Bme1580I G_kGCm'C
Cuts at: 1790 3155 3316 3701 4417 4586 4800 4930 5008
Size: 1365 161 385 716 169 214 130 78
Cuts at: 5008 5657 5848 6729 7885 1790
Size: 649 191 881 1156 2573
Fragments arranged by size:
2573 1365 1156 881 716 649 385 214
191 169 161 130 78
BmgBI CAC'GTC
Cuts at: 1318 3544 1318
Size: 2226 6442
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2941 3150 3299
Size: 416 53 83 399 24 1524 209 149
Cuts at: 3299 3332 6399 7357 442
Size: 33 3067 958 1753
Fragments arranged by size:
3067 1753 1524 958 416 399 209 149
83 53 33 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8668
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4516 4915 5392 5800 5918 4516
Size: 399 477 408 118 7266
Fragments arranged by size:
7266 477 408 399 118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2740 3657 4351 4505 4910 4961 5114
Size: 1076 425 917 694 154 405 51 153
Cuts at: 5114 5326 5564 5888 7328 1239
Size: 212 238 324 1440 2579
Fragments arranged by size:
2579 1440 1076 917 694 425 405 324
238 212 154 153 51
Bpu10I CC'TnA_GC
Cuts at: 3572 3603 4605 3572
Size: 31 1002 7635
Fragments arranged by size:
7635 1002 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2210 2231 3644 4661 6793 7658 7956 2174
Size: 36 21 1413 1017 2132 865 298 2886
Fragments arranged by size:
2886 2132 1413 1017 865 298 36 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4068 5363 7331 882
Size: 617 2569 1295 1968 2219
Fragments arranged by size:
2569 2219 1968 1295 617
BsaAI yAC'GTr
Cuts at: 493 1204 2089 6459 493
Size: 711 885 4370 2702
Fragments arranged by size:
4370 2702 885 711
BsaBI GATnn'nnATC
Cuts at: 3242 8662 3242
Size: 5420 3248
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3035 3890 4004 5134
Size: 53 83 186 936 1502 855 114 1130
Cuts at: 5134 5591 5829 6475 6857 7492 275
Size: 457 238 646 382 635 1451
Fragments arranged by size:
1502 1451 1130 936 855 646 635 457
382 238 186 114 83 53
BsaWI w'CCGG_w
Cuts at: 2775 3222 3234 3357 4119 4398 4835 7101 7499
Size: 447 12 123 762 279 437 2266 398
Cuts at: 7499 7773 7920 8331 2775
Size: 274 147 411 3112
Fragments arranged by size:
3112 2266 762 447 437 411 398 279
274 147 123 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8443
Fragments arranged by size:
8443 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3221 3281 3439 3463 3620 3623 3725 3734
Size: 2035 60 158 24 157 3 102 9
Cuts at: 3734 3755 3833 4223 4226 4229 4232 4277 4292
Size: 21 78 390 3 3 3 45 15
Cuts at: 4292 4295 4307 4310 4313 4322 4325 4328 4402
Size: 3 12 3 3 9 3 3 74
Cuts at: 4402 4508 4593 4753 4874 4934 5204 5329 5363
Size: 106 85 160 121 60 270 125 34
Cuts at: 5363 5408 1186
Size: 45 4446
Fragments arranged by size:
4446 2035 390 270 160 158 157 125
121 106 102 85 78 74 60 60
45 45 34 24 21 15 12 9
9 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2205 2404 2985 3148 3296 3686 3763 3843 3858
Size: 199 581 163 148 390 77 80 15
Cuts at: 3858 4348 4620 4714 4871 4882 5514 5548 5686
Size: 490 272 94 157 11 632 34 138
Cuts at: 5686 5847 5957 7871 8367 2205
Size: 161 110 1914 496 2506
Fragments arranged by size:
2506 1914 632 581 496 490 390 272
199 163 161 157 148 138 110 94
80 77 34 15 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2296 2441 3917 1163
Size: 573 560 145 1476 5914
Fragments arranged by size:
5914 1476 573 560 145
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 5258 6118 6879 7907 1540
Size: 275 186 21 3236 860 761 1028 2301
Fragments arranged by size:
3236 2301 1028 860 761 275 186 21
BsiHKAI G_wGCw'C
Cuts at: 2247 2393 2775 2972 3093 3660 4394 4800 6729
Size: 146 382 197 121 567 734 406 1929
Cuts at: 6729 6814 7885 2247
Size: 85 1071 3030
Fragments arranged by size:
3030 1929 1071 734 567 406 382 197
146 121 85
BsmI GAATG_Cn'
Cuts at: 3115 6179 6272 3115
Size: 3064 93 5511
Fragments arranged by size:
5511 3064 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3644 3881 3644
Size: 237 8431
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 2393 2775 2972 3093 3155 3316
Size: 399 457 146 382 197 121 62 161
Cuts at: 3316 3660 3687 3701 3717 3777 4394 4417 4502
Size: 344 27 14 16 60 617 23 85
Cuts at: 4502 4586 4689 4800 4930 4991 5008 5204 5211
Size: 84 103 111 130 61 17 196 7
Cuts at: 5211 5254 5390 5657 5668 5783 5848 5879 5987
Size: 43 136 267 11 115 65 31 108
Cuts at: 5987 6729 6814 7885 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 617 457 399 382 344
267 197 196 161 146 136 130 121
115 111 108 103 85 85 84 65
62 61 60 43 31 27 23 17
16 14 11 7
BspEI T'CCGG_A
Cuts at: 3234 4119 8331 3234
Size: 885 4212 3571
Fragments arranged by size:
4212 3571 885
BspHI T'CATG_A
Cuts at: 6557 8287 6557
Size: 1730 6938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2172 2209 2268 2361 3060 3834 6023 844
Size: 1328 37 59 93 699 774 2189 3489
Fragments arranged by size:
3489 2189 1328 774 699 93 59 37
BsrBI CCG'CTC
Cuts at: 1394 1823 3588 3754 4615 5236 5442 6555 1394
Size: 429 1765 166 861 621 206 1113 3507
Fragments arranged by size:
3507 1765 1113 861 621 429 206 166
BsrDI GCAATG_nn'
Cuts at: 65 1248 2396 2826 2907 4800 5740 7163 7337
Size: 1183 1148 430 81 1893 940 1423 174
Cuts at: 7337 65
Size: 1396
Fragments arranged by size:
1893 1423 1396 1183 1148 940 430 174
81
BsrFI r'CCGG_y
Cuts at: 1700 1810 2077 5416 5836 7312 7499 1700
Size: 110 267 3339 420 1476 187 2869
Fragments arranged by size:
3339 2869 1476 420 267 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8668
BssHII G'CGCG_C
Cuts at: 1925 5729 5914 1925
Size: 3804 185 4679
Fragments arranged by size:
4679 3804 185
BssSI C'ACGA_G
Cuts at: 2965 4032 6728 7740 2965
Size: 1067 2696 1012 3893
Fragments arranged by size:
3893 2696 1067 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2779 3835 4144 794
Size: 1985 1056 309 5318
Fragments arranged by size:
5318 1985 1056 309
BstBI TT'CG_AA
Cuts at: 7472 7472
Size: 8668
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8668
BstXI CCAn_nnnn'nTGG
Cuts at: 2089 3875 4429 4510 5855 2089
Size: 1786 554 81 1345 4902
Fragments arranged by size:
4902 1786 1345 554 81
BstYI r'GATC_y
Cuts at: 1972 2145 2415 3439 5531 5998 6365 6750 6767
Size: 173 270 1024 2092 467 367 385 17
Cuts at: 6767 8219 8305 8317 8663 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2092 1977 1452 1024 467 385 367 346
270 173 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3189 4185 4491 5212 5323 3189
Size: 996 306 721 111 6534
Fragments arranged by size:
6534 996 721 306 111
BtgI C'CryG_G
Cuts at: 513 1065 2157 2487 2562 3393 3702 3999 4467
Size: 552 1092 330 75 831 309 297 468
Cuts at: 4467 4626 5536 5644 513
Size: 159 910 108 3537
Fragments arranged by size:
3537 1092 910 831 552 468 330 309
297 159 108 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3888 487
Size: 683 56 2662 5267
Fragments arranged by size:
5267 2662 683 56
BtsI GCAGTG_nn'
Cuts at: 2015 2709 3731 4378 4551 5042 5607 6184 6978
Size: 694 1022 647 173 491 565 577 794
Cuts at: 6978 6998 2015
Size: 20 3685
Fragments arranged by size:
3685 1022 794 694 647 577 565 491
173 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2029 2064 529
Size: 35 1465 35 7133
Fragments arranged by size:
7133 1465 35 35
DraI TTT'AAA
Cuts at: 5972 5972
Size: 8668
DraIII CAC_nnn'GTG
Cuts at: 2446 2446
Size: 8668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7675 809
Size: 507 6359 1802
Fragments arranged by size:
6359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2079 2238
Size: 54 1425 50 275 18 189 60 159
Cuts at: 2238 2346 2592 3532 3728 3877 4470 5031 5115
Size: 108 246 940 196 149 593 561 84
Cuts at: 5115 5226 5255 5604 5641 6115 7004 8633 8
Size: 111 29 349 37 474 889 1629 43
Fragments arranged by size:
1629 1425 940 889 593 561 474 349
275 246 196 189 159 149 111 108
84 60 54 50 43 37 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 2019 5255 6115 1537
Size: 275 207 3236 860 4090
Fragments arranged by size:
4090 3236 860 275 207
EarI CTCTTCn'nnn_
Cuts at: 1353 2684 2819 3744 4200 4227 4236 4254 4263
Size: 1331 135 925 456 27 9 18 9
Cuts at: 4263 4269 4284 4299 5285 5432 6598 1353
Size: 6 15 15 986 147 1166 3423
Fragments arranged by size:
3423 1331 1166 986 925 456 147 135
27 18 15 15 9 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3413 3830 5612 5713 7255 7629
Size: 1064 138 1824 417 1782 101 1542 374
Cuts at: 7629 7775 387
Size: 146 1280
Fragments arranged by size:
1824 1782 1542 1280 1064 417 374 146
138 101
EcoICRI GAG'CTC
Cuts at: 3658 3658
Size: 8668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2740 2807 3216 3657 4351 4505
Size: 393 683 425 67 409 441 694 154
Cuts at: 4505 4910 4961 5114 5326 5564 5888 6731 7328
Size: 405 51 153 212 238 324 843 597
Cuts at: 7328 8115 8383 1239
Size: 787 268 1524
Fragments arranged by size:
1524 843 787 694 683 597 441 425
409 405 393 324 268 238 212 154
153 67 51
EcoNI CCTnn'n_nnAGG
Cuts at: 4707 5431 4707
Size: 724 7944
EcoO109I rG'GnC_Cy
Cuts at: 2221 3049 3135 3152 3185 3292 3568 3823 3941
Size: 828 86 17 33 107 276 255 118
Cuts at: 3941 4162 4352 4480 4700 4788 4926 5004 5005
Size: 221 190 128 220 88 138 78 1
Cuts at: 5005 5266 5328 5682 5812 5888 2221
Size: 261 62 354 130 76 5001
Fragments arranged by size:
5001 828 354 276 261 255 221 220
190 138 130 128 118 107 88 86
78 76 62 33 17 1
EcoRI G'AATT_C
Cuts at: 5977 6355 5977
Size: 378 8290
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 8668
FspI TGC'GCA
Cuts at: 7174 7174
Size: 8668
HaeII r_GCGC'y
Cuts at: 1678 3812 4007 4872 4914 4937 5359 5693 7815
Size: 2134 195 865 42 23 422 334 2122
Cuts at: 7815 1678
Size: 2531
Fragments arranged by size:
2531 2134 2122 865 422 334 195 42
23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2657 2689 3413 3445 5186
Size: 32 135 32 1085 32 724 32 1741
Cuts at: 5186 5218 7309 7341 7383 7415 1373
Size: 32 2091 32 42 32 2626
Fragments arranged by size:
2626 2091 1741 1085 724 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3220 6012 6258 669
Size: 988 281 1282 2792 246 3079
Fragments arranged by size:
3079 2792 1282 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8668
HpaI GTT'AAC
Cuts at: 6258 6258
Size: 8668
KasI G'GCGC_C
Cuts at: 4003 4003
Size: 8668
KpnI G_GTAC'C
Cuts at: 5993 5993
Size: 8668
MfeI C'AATT_G
Cuts at: 6267 6267
Size: 8668
MluI A'CGCG_T
Cuts at: 8544 8544
Size: 8668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3945 7782 7966 1305
Size: 2640 3837 184 2007
Fragments arranged by size:
3837 2640 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5033 5117 10
Size: 54 1425 343 3201 84 3561
Fragments arranged by size:
3561 3201 1425 343 84 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2690 2876 3096 3542 5498 6626 6985
Size: 648 1524 186 220 446 1956 1128 359
Cuts at: 6985 7144 518
Size: 159 2042
Fragments arranged by size:
2042 1956 1524 1128 648 446 359 220
186 159
MspA1I CmG'CkG
Cuts at: 1606 2713 3598 3862 4325 4639 5092 5423 5584
Size: 1107 885 264 463 314 453 331 161
Cuts at: 5584 5646 6084 6761 7909 8154 1606
Size: 62 438 677 1148 245 2120
Fragments arranged by size:
2120 1148 1107 885 677 463 453 438
331 314 264 245 161 62
NaeI GCC'GGC
Cuts at: 2079 5418 5838 2079
Size: 3339 420 4909
Fragments arranged by size:
4909 3339 420
NarI GG'CG_CC
Cuts at: 4004 4004
Size: 8668
NcoI C'CATG_G
Cuts at: 513 1065 2157 2487 4467 5536 513
Size: 552 1092 330 1980 1069 3645
Fragments arranged by size:
3645 1980 1092 1069 552 330
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8668
NgoMIV G'CCGG_C
Cuts at: 2077 5416 5836 2077
Size: 3339 420 4909
Fragments arranged by size:
4909 3339 420
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8668
NotI GC'GGCC_GC
Cuts at: 2019 6115 2019
Size: 4096 4572
NspI r_CATG'y
Cuts at: 1132 1229 2689 2797 3805 4144 5497 6026 7571
Size: 97 1460 108 1008 339 1353 529 1545
Cuts at: 7571 1132
Size: 2229
Fragments arranged by size:
2229 1545 1460 1353 1008 529 339 108
97
PasI CC'CwG_GG
Cuts at: 1348 5597 5657 1348
Size: 4249 60 4359
Fragments arranged by size:
4359 4249 60
PciI A'CATG_T
Cuts at: 7567 7567
Size: 8668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2446 3044 1108
Size: 1338 598 6732
Fragments arranged by size:
6732 1338 598
PfoI T'CCnGG_A
Cuts at: 2817 3057 2817
Size: 240 8428
PmeI GTTT'AAAC
Cuts at: 5972 5972
Size: 8668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2595 2627 6737 6769 8250 8282 2595
Size: 32 4110 32 1481 32 2981
Fragments arranged by size:
4110 2981 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2221 3049 3185 3292 3823 3941 4162 4480 4788
Size: 828 136 107 531 118 221 318 308
Cuts at: 4788 5266 5812 5888 2221
Size: 478 546 76 5001
Fragments arranged by size:
5001 828 546 531 478 318 308 221
136 118 107 76
PshAI GACnn'nnGTC
Cuts at: 1613 3215 1613
Size: 1602 7066
PsiI TTA'TAA
Cuts at: 6238 6238
Size: 8668
PspOMI G'GGCC_C
Cuts at: 3151 4926 5004 5653 5844 3151
Size: 1775 78 649 191 5975
Fragments arranged by size:
5975 1775 649 191 78
PstI C_TGCA'G
Cuts at: 830 3831 4324 5589 6020 830
Size: 3001 493 1265 431 3478
Fragments arranged by size:
3478 3001 1265 493 431
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8668
PvuII CAG'CTG
Cuts at: 2713 3862 4639 5584 2713
Size: 1149 777 945 5797
Fragments arranged by size:
5797 1149 945 777
SacI G_AGCT'C
Cuts at: 3660 3660
Size: 8668
SacII CC_GC'GG
Cuts at: 5647 5647
Size: 8668
SalI G'TCGA_C
Cuts at: 1936 6010 1936
Size: 4074 4594
SanDI GG'GwC_CC
Cuts at: 3292 3941 5812 3292
Size: 649 1871 6148
Fragments arranged by size:
6148 1871 649
SapI GCTCTTCn'nnn_
Cuts at: 3744 5432 3744
Size: 1688 6980
SbfI CC_TGCA'GG
Cuts at: 6020 6020
Size: 8668
ScaI AGT'ACT
Cuts at: 6916 8628 6916
Size: 1712 6956
SfcI C'TryA_G
Cuts at: 826 1045 2509 3080 3827 4320 5585 6016 7151
Size: 219 1464 571 747 493 1265 431 1135
Cuts at: 7151 7832 8023 826
Size: 681 191 1471
Fragments arranged by size:
1471 1464 1265 1135 747 681 571 493
431 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4469 5327 4469
Size: 858 7810
SfoI GGC'GCC
Cuts at: 4005 4005
Size: 8668
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8668
SmaI CCC'GGG
Cuts at: 1872 3130 3181 3791 4698 5012 5809 5825 5843
Size: 1258 51 610 907 314 797 16 18
Cuts at: 5843 5872 5995 1872
Size: 29 123 4545
Fragments arranged by size:
4545 1258 907 797 610 314 123 51
29 18 16
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2027 2189 2225 2246 3659 4676
Size: 197 926 84 162 36 21 1413 1017
Cuts at: 4676 6772 7673 7935 820
Size: 2096 901 262 1553
Fragments arranged by size:
2096 1553 1413 1017 926 901 262 197
162 84 36 21
SnaBI TAC'GTA
Cuts at: 493 6459 493
Size: 5966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8668
SphI G_CATG'C
Cuts at: 1132 3805 5497 6026 1132
Size: 2673 1692 529 3774
Fragments arranged by size:
3774 2673 1692 529
SrfI GCCC'GGGC
Cuts at: 5843 5843
Size: 8668
SspI AAT'ATT
Cuts at: 5 52 6592 5
Size: 47 6540 2081
Fragments arranged by size:
6540 2081 47
StuI AGG'CCT
Cuts at: 1517 2924 3467 1517
Size: 1407 543 6718
Fragments arranged by size:
6718 1407 543
StyI C'CwwG_G
Cuts at: 513 1065 2157 2487 4335 4467 5536 6107 513
Size: 552 1092 330 1848 132 1069 571 3074
Fragments arranged by size:
3074 1848 1092 1069 571 552 330 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3534 6695 6712 6865 7050 7495 325
Size: 1255 1954 3161 17 153 185 445 1498
Fragments arranged by size:
3161 1954 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2550 6914 8626
Size: 274 80 33 51 1443 573 4364 1712
Cuts at: 8626 96
Size: 138
Fragments arranged by size:
4364 1712 1443 573 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2258 2290 8628 8660 2258
Size: 32 6338 32 2266
Fragments arranged by size:
6338 2266 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8668
XbaI T'CTAG_A
Cuts at: 6004 6004
Size: 8668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 2401 2443 3019 3535 2179
Size: 222 42 576 516 7312
Fragments arranged by size:
7312 576 516 222 42
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8668
XmaI C'CCGG_G
Cuts at: 1870 3128 3179 3789 4696 5010 5807 5823 5841
Size: 1258 51 610 907 314 797 16 18
Cuts at: 5841 5870 5993 1870
Size: 29 123 4545
Fragments arranged by size:
4545 1258 907 797 610 314 123 51
29 18 16
XmnI GAAnn'nnTTC
Cuts at: 6797 6797
Size: 8668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3891 6476 276
Size: 53 83 186 936 2357 2585 2468
Fragments arranged by size:
2585 2468 2357 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SexAI SwaI