Restriction map for
pFN21AE3447
(Circular) (Six-base) MAPSORT of: pFN21AE3447.seq Check: 8843 from: 1 to: 8548
pFN21AE3447 8548 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3017 3100 4195 5538 3017
Size: 83 1095 1343 6027
Fragments arranged by size:
6027 1343 1095 83
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6358 278
Size: 53 83 186 936 4822 2468
Fragments arranged by size:
4822 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3635 5552 5891 1937
Size: 1698 1917 339 4594
Fragments arranged by size:
4594 1917 1698 339
Acc65I G'GTAC_C
Cuts at: 3516 5869 3516
Size: 2353 6195
AclI AA'CG_TT
Cuts at: 1563 2550 6675 7048 1563
Size: 987 4125 373 3063
Fragments arranged by size:
4125 3063 987 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3387 3564 3925 5018 5247 6611 7995 8263
Size: 1755 177 361 1093 229 1364 1384 268
Cuts at: 8263 1632
Size: 1917
Fragments arranged by size:
1917 1755 1384 1364 1093 361 268 229
177
AflII C'TTAA_G
Cuts at: 820 1017 3164 3533 820
Size: 197 2147 369 5835
Fragments arranged by size:
5835 2147 369 197
AflIII A'CryG_T
Cuts at: 1203 3657 3858 3901 7447 8424 1203
Size: 2454 201 43 3546 977 1327
Fragments arranged by size:
3546 2454 1327 977 201 43
AgeI A'CCGG_T
Cuts at: 7379 7379
Size: 8548
AhdI GACnn_n'nnGTC
Cuts at: 7277 7277
Size: 8548
AleI CACnn'nnGTG
Cuts at: 1166 2699 5672 1166
Size: 1533 2973 4042
Fragments arranged by size:
4042 2973 1533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4652 5690 1184
Size: 3468 1038 4042
Fragments arranged by size:
4042 3468 1038
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2876 2908 2876
Size: 32 8516
AlwNI CAG_nnn'CTG
Cuts at: 2064 2453 2483 2533 4007 4091 4690 5051 6291
Size: 389 30 50 1474 84 599 361 1240
Cuts at: 6291 7863 2064
Size: 1572 2749
Fragments arranged by size:
2749 1572 1474 1240 599 389 361 84
50 30
ApaI G_GGCC'C
Cuts at: 5703 5703
Size: 8548
ApaLI G'TGCA_C
Cuts at: 3962 5675 6605 7761 3962
Size: 1713 930 1156 4749
Fragments arranged by size:
4749 1713 1156 930
ApoI r'AATT_y
Cuts at: 1493 2407 3065 4482 5857 6083 6235 8344 1493
Size: 914 658 1417 1375 226 152 2109 1697
Fragments arranged by size:
2109 1697 1417 1375 914 658 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7102 160
Size: 872 6070 1606
Fragments arranged by size:
6070 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8548
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 2491 5781 5873 1870
Size: 73 195 353 3290 92 4545
Fragments arranged by size:
4545 3290 353 195 92 73
AvrII C'CTAG_G
Cuts at: 2803 2803
Size: 8548
BamHI G'GATC_C
Cuts at: 2022 5114 5878 6245 2022
Size: 3092 764 367 4325
Fragments arranged by size:
4325 3092 764 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 3516 4273 4390 5159
Size: 325 217 627 254 1475 757 117 769
Cuts at: 5159 5499 5869 7324 618
Size: 340 370 1455 1842
Fragments arranged by size:
1842 1475 1455 769 757 627 370 340
325 254 217 117
BanII G_rGCy'C
Cuts at: 1391 2301 3298 5317 5591 5703 5867 1391
Size: 910 997 2019 274 112 164 4072
Fragments arranged by size:
4072 2019 997 910 274 164 112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5769 928
Size: 971 3870 3707
Fragments arranged by size:
3870 3707 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6739 6773 1903
Size: 34 4802 34 3678
Fragments arranged by size:
4802 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5798 6440 7650 5798
Size: 642 1210 6696
Fragments arranged by size:
6696 1210 642
BclI T'GATC_A
Cuts at: 1548 3412 1548
Size: 1864 6684
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5726 7159 136
Size: 107 122 71 631 605 4054 1433 1525
Fragments arranged by size:
4054 1525 1433 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3837 8543 3837
Size: 4706 3842
BlpI GC'TnA_GC
Cuts at: 3366 4284 4683 5965 3366
Size: 918 399 1282 5949
Fragments arranged by size:
5949 1282 918 399
Bme1580I G_kGCm'C
Cuts at: 1790 3966 4395 5502 5679 5703 6609 7765 1790
Size: 2176 429 1107 177 24 906 1156 2573
Fragments arranged by size:
2573 2176 1156 1107 906 429 177 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8548
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3359 4977 5594
Size: 416 53 83 399 24 1942 1618 617
Cuts at: 5594 6279 7237 442
Size: 685 958 1753
Fragments arranged by size:
1942 1753 1618 958 685 617 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4589 1056
Size: 3533 5015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3578 3665 4113 4488 4615 4752 5028 7208
Size: 2339 87 448 375 127 137 276 2180
Cuts at: 7208 1239
Size: 2579
Fragments arranged by size:
2579 2339 2180 448 375 276 137 127
87
Bpu10I CC'TnA_GC
Cuts at: 2939 3621 5237 2939
Size: 682 1616 6250
Fragments arranged by size:
6250 1616 682
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2582 3068 3119 4344 5617 6673 7538 7836 2582
Size: 486 51 1225 1273 1056 865 298 3294
Fragments arranged by size:
3294 1273 1225 1056 865 486 298 51
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5237 7211 882
Size: 617 3738 1974 2219
Fragments arranged by size:
3738 2219 1974 617
BsaAI yAC'GTr
Cuts at: 493 1204 5556 6339 493
Size: 711 4352 783 2702
Fragments arranged by size:
4352 2702 783 711
BsaBI GATnn'nnATC
Cuts at: 4884 8542 4884
Size: 3658 4890
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6355 6737 7372 275
Size: 53 83 186 936 4822 382 635 1451
Fragments arranged by size:
4822 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6981 7379 7653 7800 8211 6981
Size: 398 274 147 411 7318
Fragments arranged by size:
7318 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2100 2130 2876 2906 1178
Size: 30 165 30 697 30 746 30 6820
Fragments arranged by size:
6820 746 697 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2380 2429 2432 2860 5699 1186
Size: 1194 49 3 428 2839 4035
Fragments arranged by size:
4035 2839 1194 428 49 3
BseYI C'CCAG_C
Cuts at: 2307 2365 2446 4101 4913 5410 5702 7751 8247
Size: 58 81 1655 812 497 292 2049 496
Cuts at: 8247 2307
Size: 2608
Fragments arranged by size:
2608 2049 1655 812 497 496 292 81
58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 2645 4065 5191 5355 5565 1163
Size: 573 450 459 1420 1126 164 210 4146
Fragments arranged by size:
4146 1420 1126 573 459 450 210 164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2179 4420 4424 5998 6759 7787
Size: 275 186 178 2241 4 1574 761 1028
Cuts at: 7787 1540
Size: 2301
Fragments arranged by size:
2301 2241 1574 1028 761 275 186 178
4
BsiHKAI G_wGCw'C
Cuts at: 2852 3266 3298 3966 5098 5317 5679 6609 6694
Size: 414 32 668 1132 219 362 930 85
Cuts at: 6694 7765 2852
Size: 1071 3635
Fragments arranged by size:
3635 1132 1071 930 668 414 362 219
85 32
BsmI GAATG_Cn'
Cuts at: 4222 4606 6059 6152 4222
Size: 384 1453 93 6618
Fragments arranged by size:
6618 1453 384 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2301 2852 3266 3298 3966 4395 5098
Size: 399 511 551 414 32 668 429 703
Cuts at: 5098 5317 5502 5591 5679 5703 5867 6609 6694
Size: 219 185 89 88 24 164 742 85
Cuts at: 6694 7765 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 703 668 551 511 429
414 399 219 185 164 89 88 85
32 24
BspEI T'CCGG_A
Cuts at: 8211 8211
Size: 8548
BspHI T'CATG_A
Cuts at: 6437 8167 6437
Size: 1730 6818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3017 3100 4195 5538 5903 844
Size: 2173 83 1095 1343 365 3489
Fragments arranged by size:
3489 2173 1343 1095 365 83
BsrBI CCG'CTC
Cuts at: 1394 1823 6435 1394
Size: 429 4612 3507
Fragments arranged by size:
4612 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4557 5601 7043 7217 65
Size: 1183 3309 1044 1442 174 1396
Fragments arranged by size:
3309 1442 1396 1183 1044 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4415 4419 4423 7192 7379 1700
Size: 110 2605 4 4 2769 187 2869
Fragments arranged by size:
2869 2769 2605 187 110 4 4
BsrGI T'GTAC_A
Cuts at: 96 4328 96
Size: 4232 4316
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8548
BssSI C'ACGA_G
Cuts at: 6608 7620 6608
Size: 1012 7536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2064 4007 4280 4510 794
Size: 1270 1943 273 230 4832
Fragments arranged by size:
4832 1943 1270 273 230
BstBI TT'CG_AA
Cuts at: 4745 7352 4745
Size: 2607 5941
BstEII G'GTnAC_C
Cuts at: 1184 3315 1184
Size: 2131 6417
BstXI CCAn_nnnn'nTGG
Cuts at: 4613 4613
Size: 8548
BstYI r'GATC_y
Cuts at: 1972 2022 2760 3502 3837 4153 5114 5878 6245
Size: 50 738 742 335 316 961 764 367
Cuts at: 6245 6630 6647 8099 8185 8197 8543 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 961 764 742 738 385 367
346 335 316 86 50 17 12
BstZ17I GTA'TAC
Cuts at: 5553 5553
Size: 8548
Bsu36I CC'TnA_GG
Cuts at: 5227 5227
Size: 8548
BtgI C'CryG_G
Cuts at: 513 1065 4566 513
Size: 552 3501 4495
Fragments arranged by size:
4495 3501 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7809
Fragments arranged by size:
7809 683 56
BtsI GCAGTG_nn'
Cuts at: 2074 2281 2463 2503 2950 3074 4677 6064 6858
Size: 207 182 40 447 124 1603 1387 794
Cuts at: 6858 6878 2074
Size: 20 3744
Fragments arranged by size:
3744 1603 1387 794 447 207 182 124
40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8513
DraI TTT'AAA
Cuts at: 2049 5852 2049
Size: 3803 4745
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7555 809
Size: 507 6239 1802
Fragments arranged by size:
6239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3114 4417 4421
Size: 54 1425 50 275 18 1284 1303 4
Cuts at: 4421 4674 5995 6884 8513 8
Size: 253 1321 889 1629 43
Fragments arranged by size:
1629 1425 1321 1303 1284 889 275 253
54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 4417 4421 5995 1537
Size: 275 2605 4 1574 4090
Fragments arranged by size:
4090 2605 1574 275 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2837 3676 4780 4956 5062 6478 1353
Size: 1484 839 1104 176 106 1416 3423
Fragments arranged by size:
3423 1484 1416 1104 839 176 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2198 7135 7509 7655 387
Size: 1064 138 609 4937 374 146 1280
Fragments arranged by size:
4937 1280 1064 609 374 146 138
EcoICRI GAG'CTC
Cuts at: 3296 5315 3296
Size: 2019 6529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3387 3564 3578 3665 3925 4113 4488
Size: 393 1755 177 14 87 260 188 375
Cuts at: 4488 4615 4752 5018 5028 5247 6611 7208 7995
Size: 127 137 266 10 219 1364 597 787
Cuts at: 7995 8263 1239
Size: 268 1524
Fragments arranged by size:
1755 1524 1364 787 597 393 375 268
266 260 219 188 177 137 127 87
14 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2138 2138
Size: 8548
EcoO109I rG'GnC_Cy
Cuts at: 2093 2434 5129 5683 2093
Size: 341 2695 554 4958
Fragments arranged by size:
4958 2695 554 341
EcoRI G'AATT_C
Cuts at: 5857 6235 5857
Size: 378 8170
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3907 3907
Size: 8548
FseI GG_CCGG'CC
Cuts at: 4423 4423
Size: 8548
FspI TGC'GCA
Cuts at: 4411 7054 4411
Size: 2643 5905
HaeII r_GCGC'y
Cuts at: 1678 4430 7695 1678
Size: 2752 3265 2531
Fragments arranged by size:
3265 2752 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2521 2553 3130 3162 3204
Size: 32 135 32 949 32 577 32 42
Cuts at: 3204 3236 3329 3361 3763 3795 3871 3903 4129
Size: 32 93 32 402 32 76 32 226
Cuts at: 4129 4161 5039 5071 5277 5309 7189 7221 7263
Size: 32 878 32 206 32 1880 32 42
Cuts at: 7263 7295 1373
Size: 32 2626
Fragments arranged by size:
2626 1880 949 878 577 402 226 206
135 93 76 42 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2608 5892 6138 669
Size: 988 281 670 3284 246 3079
Fragments arranged by size:
3284 3079 988 670 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8548
HpaI GTT'AAC
Cuts at: 6138 6138
Size: 8548
KpnI G_GTAC'C
Cuts at: 3520 5873 3520
Size: 2353 6195
MfeI C'AATT_G
Cuts at: 3717 4260 6147 3717
Size: 543 1887 6118
Fragments arranged by size:
6118 1887 543
MluI A'CGCG_T
Cuts at: 8424 8424
Size: 8548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2000 3009 4186 5408 7662 7846 1305
Size: 695 1009 1177 1222 2254 184 2007
Fragments arranged by size:
2254 2007 1222 1177 1009 695 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3116 4676 10
Size: 54 1425 343 1284 1560 3882
Fragments arranged by size:
3882 1560 1425 1284 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2699 2983 3861 4550 4611 4634 5284
Size: 648 1533 284 878 689 61 23 650
Cuts at: 5284 5672 6506 6865 7024 518
Size: 388 834 359 159 2042
Fragments arranged by size:
2042 1533 878 834 689 650 648 388
359 284 159 61 23
MspA1I CmG'CkG
Cuts at: 1606 2200 2230 2483 2727 2746 3452 4007 4280
Size: 594 30 253 244 19 706 555 273
Cuts at: 4280 4448 5812 5964 6641 7789 8034 1606
Size: 168 1364 152 677 1148 245 2120
Fragments arranged by size:
2120 1364 1148 706 677 594 555 273
253 245 244 168 152 30 19
NaeI GCC'GGC
Cuts at: 4417 4421 4425 4417
Size: 4 4 8540
Fragments arranged by size:
8540 4 4
NcoI C'CATG_G
Cuts at: 513 1065 4566 513
Size: 552 3501 4495
Fragments arranged by size:
4495 3501 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8548
NgoMIV G'CCGG_C
Cuts at: 4415 4419 4423 4415
Size: 4 4 8540
Fragments arranged by size:
8540 4 4
NheI G'CTAG_C
Cuts at: 1052 4585 1052
Size: 3533 5015
NotI GC'GGCC_GC
Cuts at: 5995 5995
Size: 8548
NspI r_CATG'y
Cuts at: 1132 1229 3661 3862 3905 5642 5906 7451 1132
Size: 97 2432 201 43 1737 264 1545 2229
Fragments arranged by size:
2432 2229 1737 1545 264 201 97 43
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8548
PciI A'CATG_T
Cuts at: 3657 3858 3901 7447 3657
Size: 201 43 3546 4758
Fragments arranged by size:
4758 3546 201 43
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4091 1108
Size: 2983 5565
PfoI T'CCnGG_A
Cuts at: 4090 4090
Size: 8548
PmeI GTTT'AAAC
Cuts at: 5852 5852
Size: 8548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2704 2736 2876 2908 6617 6649 8130 8162 2704
Size: 32 140 32 3709 32 1481 32 3090
Fragments arranged by size:
3709 3090 1481 140 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5683 5683
Size: 8548
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8548
PsiI TTA'TAA
Cuts at: 4857 6118 4857
Size: 1261 7287
PspOMI G'GGCC_C
Cuts at: 5699 5699
Size: 8548
PspXI vC'TCGA_Gb
Cuts at: 2138 2138
Size: 8548
PstI C_TGCA'G
Cuts at: 830 2069 2276 2458 4003 4458 5647 5900 830
Size: 1239 207 182 1545 455 1189 253 3478
Fragments arranged by size:
3478 1545 1239 1189 455 253 207 182
PvuI CG_AT'CG
Cuts at: 2001 2179 2001
Size: 178 8370
PvuII CAG'CTG
Cuts at: 2200 2230 2746 3452 4007 4280 2200
Size: 30 516 706 555 273 6468
Fragments arranged by size:
6468 706 555 516 273 30
SacI G_AGCT'C
Cuts at: 3298 5317 3298
Size: 2019 6529
SalI G'TCGA_C
Cuts at: 1936 5890 1936
Size: 3954 4594
SbfI CC_TGCA'GG
Cuts at: 5900 5900
Size: 8548
ScaI AGT'ACT
Cuts at: 2169 6796 8508 2169
Size: 4627 1712 2209
Fragments arranged by size:
4627 2209 1712
SexAI A'CCwGG_T
Cuts at: 2397 3485 5679 2397
Size: 1088 2194 5266
Fragments arranged by size:
5266 2194 1088
SfcI C'TryA_G
Cuts at: 826 1045 2065 2272 2454 3289 3636 3999 4292
Size: 219 1020 207 182 835 347 363 293
Cuts at: 4292 4454 5211 5643 5896 7031 7712 7903 826
Size: 162 757 432 253 1135 681 191 1471
Fragments arranged by size:
1471 1135 1020 835 757 681 432 363
347 293 253 219 207 191 182 162
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8548
SmaI CCC'GGG
Cuts at: 1872 5875 1872
Size: 4003 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2138 2561 3083 3134 3164 3533
Size: 197 926 195 423 522 51 30 369
Cuts at: 3533 4359 5632 6652 7553 7815 820
Size: 826 1273 1020 901 262 1553
Fragments arranged by size:
1553 1273 1020 926 901 826 522 423
369 262 197 195 51 30
SnaBI TAC'GTA
Cuts at: 493 5556 6339 493
Size: 5063 783 2702
Fragments arranged by size:
5063 2702 783
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8548
SphI G_CATG'C
Cuts at: 1132 5642 5906 1132
Size: 4510 264 3774
Fragments arranged by size:
4510 3774 264
SspI AAT'ATT
Cuts at: 5 52 2342 6472 5
Size: 47 2290 4130 2081
Fragments arranged by size:
4130 2290 2081 47
StuI AGG'CCT
Cuts at: 1517 5225 1517
Size: 3708 4840
StyI C'CwwG_G
Cuts at: 513 1065 2803 2853 4566 4863 5003 5987 513
Size: 552 1738 50 1713 297 140 984 3074
Fragments arranged by size:
3074 1738 1713 984 552 297 140 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2666 3693 4148 5517 5774 6575 6592
Size: 1255 1086 1027 455 1369 257 801 17
Cuts at: 6592 6745 6930 7375 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1369 1255 1086 1027 801 455 445
257 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2167 3087 3591
Size: 274 80 33 51 1443 190 920 504
Cuts at: 3591 4328 4450 4623 6794 8506 96
Size: 737 122 173 2171 1712 138
Fragments arranged by size:
2171 1712 1443 920 737 504 274 190
173 138 122 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2100 2132 4221 4253 8508 8540 2100
Size: 32 2089 32 4255 32 2108
Fragments arranged by size:
4255 2108 2089 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8548
XbaI T'CTAG_A
Cuts at: 4789 5884 4789
Size: 1095 7453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2860 5768 2860
Size: 2908 5640
XhoI C'TCGA_G
Cuts at: 1943 2138 1943
Size: 195 8353
XmaI C'CCGG_G
Cuts at: 1870 5873 1870
Size: 4003 4545
XmnI GAAnn'nnTTC
Cuts at: 3306 3459 6677 3306
Size: 153 3218 5177
Fragments arranged by size:
5177 3218 153
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6356 276
Size: 53 83 186 936 4822 2468
Fragments arranged by size:
4822 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI
BsmBI DraIII EcoRV FspAI KasI NarI NruI NsiI
PacI PmlI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI