Restriction map for
pFN21AE3441
(Circular) (Six-base) MAPSORT of: pFN21AE3441.seq Check: 2369 from: 1 to: 8587
pFN21AE3441 8587 bp
With 182 enzymes: SgfI *
March 28, 2012 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2959 4002 2959
Size: 1043 7544
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6397 278
Size: 53 83 186 936 4861 2468
Fragments arranged by size:
4861 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4281 5930 1937
Size: 2344 1649 4594
Fragments arranged by size:
4594 2344 1649
Acc65I G'GTAC_C
Cuts at: 5908 5908
Size: 8587
AclI AA'CG_TT
Cuts at: 1563 2076 6714 7087 1563
Size: 513 4638 373 3063
Fragments arranged by size:
4638 3063 513 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2598 2684 2961 3092 3150 4258 4901 5232
Size: 966 86 277 131 58 1108 643 331
Cuts at: 5232 6650 8034 8302 1632
Size: 1418 1384 268 1917
Fragments arranged by size:
1917 1418 1384 1108 966 643 331 277
268 131 86 58
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8390
AflIII A'CryG_T
Cuts at: 1203 4797 7486 8463 1203
Size: 3594 2689 977 1327
Fragments arranged by size:
3594 2689 1327 977
AgeI A'CCGG_T
Cuts at: 7418 7418
Size: 8587
AhdI GACnn_n'nnGTC
Cuts at: 7316 7316
Size: 8587
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4129 4671 1184
Size: 2945 542 5100
Fragments arranged by size:
5100 2945 542
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4378 4410 5209 5241 4378
Size: 32 799 32 7724
Fragments arranged by size:
7724 799 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2104 2396 4325 4696 6330 7902 2104
Size: 292 1929 371 1634 1572 2789
Fragments arranged by size:
2789 1929 1634 1572 371 292
ApaI G_GGCC'C
Cuts at: 4639 4639
Size: 8587
ApaLI G'TGCA_C
Cuts at: 3828 5696 6644 7800 3828
Size: 1868 948 1156 4615
Fragments arranged by size:
4615 1868 1156 948
ApoI r'AATT_y
Cuts at: 1493 2150 3050 3353 5028 5896 6122 6274 8383
Size: 657 900 303 1675 868 226 152 2109
Cuts at: 8383 1493
Size: 1697
Fragments arranged by size:
2109 1697 1675 900 868 657 303 226
152
AseI AT'TA_AT
Cuts at: 160 1032 3486 7141 160
Size: 872 2454 3655 1606
Fragments arranged by size:
3655 2454 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8587
AvaI C'yCGr_G
Cuts at: 1870 1943 3547 5912 1870
Size: 73 1604 2365 4545
Fragments arranged by size:
4545 2365 1604 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3703 3736 4030 4063 3703
Size: 33 294 33 8227
Fragments arranged by size:
8227 294 33 33
BamHI G'GATC_C
Cuts at: 5917 6284 5917
Size: 367 8220
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2680 4499 4609 5579 5602
Size: 325 217 627 893 1819 110 970 23
Cuts at: 5602 5908 7363 618
Size: 306 1455 1842
Fragments arranged by size:
1842 1819 1455 970 893 627 325 306
217 110 23
BanII G_rGCy'C
Cuts at: 1391 2415 2579 3479 3548 4263 4371 4639 5906
Size: 1024 164 900 69 715 108 268 1267
Cuts at: 5906 1391
Size: 4072
Fragments arranged by size:
4072 1267 1024 900 715 268 164 108
69
BbeI G_GCGC'C
Cuts at: 4613 4613
Size: 8587
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7616
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6778 6812 1903
Size: 34 50 34 4757 34 3678
Fragments arranged by size:
4757 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4662 6479 7689 4662
Size: 1817 1210 5560
Fragments arranged by size:
5560 1817 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8587
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4556 7198 136
Size: 107 122 71 631 605 2884 2642 1525
Fragments arranged by size:
2884 2642 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2755 8582 2755
Size: 5827 2760
BlpI GC'TnA_GC
Cuts at: 2817 4923 5091 6004 2817
Size: 2106 168 913 5400
Fragments arranged by size:
5400 2106 913 168
Bme1580I G_kGCm'C
Cuts at: 1790 3291 3832 4639 5700 6648 7804 1790
Size: 1501 541 807 1061 948 1156 2573
Fragments arranged by size:
2573 1501 1156 1061 948 807 541
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8587
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2310 2573 4684
Size: 416 53 83 399 24 893 263 2111
Cuts at: 4684 6318 7276 442
Size: 1634 958 1753
Fragments arranged by size:
2111 1753 1634 958 893 416 399 263
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8587
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3193 3193
Size: 8587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2373 2507 2676 3237 3559 4187 4281
Size: 887 247 134 169 561 322 628 94
Cuts at: 4281 5202 5404 7247 1239
Size: 921 202 1843 2579
Fragments arranged by size:
2579 1843 921 887 628 561 322 247
202 169 134 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2436 5446 5655 6712 7577 7875 2436
Size: 3010 209 1057 865 298 3148
Fragments arranged by size:
3148 3010 1057 865 298 209
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5254 7250 882
Size: 617 3755 1996 2219
Fragments arranged by size:
3755 2219 1996 617
BsaAI yAC'GTr
Cuts at: 493 1204 5434 6378 493
Size: 711 4230 944 2702
Fragments arranged by size:
4230 2702 944 711
BsaBI GATnn'nnATC
Cuts at: 8581 8581
Size: 8587
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4487 4610 6394 6776
Size: 53 83 186 936 2954 123 1784 382
Cuts at: 6776 7411 275
Size: 635 1451
Fragments arranged by size:
2954 1784 1451 936 635 382 186 123
83 53
BsaWI w'CCGG_w
Cuts at: 2049 4718 7020 7418 7692 7839 8250 2049
Size: 2669 2302 398 274 147 411 2386
Fragments arranged by size:
2669 2386 2302 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2321 2351 4305 4335 5567
Size: 30 165 30 918 30 1954 30 1232
Cuts at: 5567 5597 1178
Size: 30 4168
Fragments arranged by size:
4168 1954 1232 918 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2415 3136 3920 4271 4897 4973 5002 5484
Size: 1229 721 784 351 626 76 29 482
Cuts at: 5484 1186
Size: 4289
Fragments arranged by size:
4289 1229 784 721 626 482 351 76
29
BseYI C'CCAG_C
Cuts at: 2024 2396 4644 5039 7790 8286 2024
Size: 372 2248 395 2751 496 2325
Fragments arranged by size:
2751 2325 2248 496 395 372
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2449 2876 2987 3180 3449 3827 4153
Size: 573 713 427 111 193 269 378 326
Cuts at: 4153 5797 1163
Size: 1644 3953
Fragments arranged by size:
3953 1644 713 573 427 378 326 269
193 111
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6037 6798 7826 1540
Size: 275 186 4036 761 1028 2301
Fragments arranged by size:
4036 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3548 3832 4108 4173 5700 6648 6733 7804 3548
Size: 284 276 65 1527 948 85 1071 4331
Fragments arranged by size:
4331 1527 1071 948 284 276 85 65
BsmI GAATG_Cn'
Cuts at: 4777 5551 6098 6191 4777
Size: 774 547 93 7173
Fragments arranged by size:
7173 774 547 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2415 2579 3291 3479 3548 3832 4108
Size: 399 625 164 712 188 69 284 276
Cuts at: 4108 4173 4263 4371 4639 5700 5906 6648 6733
Size: 65 90 108 268 1061 206 742 85
Cuts at: 6733 7804 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1061 742 712 625 399 284
276 268 206 188 164 108 90 85
69 65
BspEI T'CCGG_A
Cuts at: 2049 8250 2049
Size: 6201 2386
BspHI T'CATG_A
Cuts at: 6476 8206 6476
Size: 1730 6857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2959 4002 5272 5942 844
Size: 2115 1043 1270 670 3489
Fragments arranged by size:
3489 2115 1270 1043 670
BsrBI CCG'CTC
Cuts at: 1394 1823 6474 1394
Size: 429 4651 3507
Fragments arranged by size:
4651 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4420 4960 7082 7256 65
Size: 1183 3172 540 2122 174 1396
Fragments arranged by size:
3172 2122 1396 1183 540 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7231 7418 1700
Size: 110 5421 187 2869
Fragments arranged by size:
5421 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5341 96
Size: 5245 3342
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8587
BssSI C'ACGA_G
Cuts at: 6647 7659 6647
Size: 1012 7575
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5146 5579 5784 794
Size: 4352 433 205 3597
Fragments arranged by size:
4352 3597 433 205
BstBI TT'CG_AA
Cuts at: 7391 7391
Size: 8587
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8587
BstYI r'GATC_y
Cuts at: 1972 2100 2239 2755 5185 5917 6284 6669 6686
Size: 128 139 516 2430 732 367 385 17
Cuts at: 6686 8138 8224 8236 8582 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2430 1977 1452 732 516 385 367 346
139 128 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2473 5169 2473
Size: 2696 5891
BtgI C'CryG_G
Cuts at: 513 1065 3649 5752 513
Size: 552 2584 2103 3348
Fragments arranged by size:
3348 2584 2103 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7848
Fragments arranged by size:
7848 683 56
BtsI GCAGTG_nn'
Cuts at: 3432 4186 6103 6897 6917 3432
Size: 754 1917 794 20 5102
Fragments arranged by size:
5102 1917 794 754 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8587
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2704 2739 4783 4818 529
Size: 35 2140 35 2044 35 4298
Fragments arranged by size:
4298 2140 2044 35 35 35
DraI TTT'AAA
Cuts at: 2710 4842 5891 2710
Size: 2132 1049 5406
Fragments arranged by size:
5406 2132 1049
DraIII CAC_nnn'GTG
Cuts at: 2549 2549
Size: 8587
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4277 4492 7594 809
Size: 507 2961 215 3102 1802
Fragments arranged by size:
3102 2961 1802 507 215
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6034 6923 8552
Size: 54 1425 50 275 18 4204 889 1629
Cuts at: 8552 8
Size: 43
Fragments arranged by size:
4204 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6034 1537
Size: 275 4222 4090
Fragments arranged by size:
4222 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4074 4218 4334 4350 6517 1353
Size: 2721 144 116 16 2167 3423
Fragments arranged by size:
3423 2721 2167 144 116 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4635 4797 7174 7548 7694 387
Size: 1064 138 3046 162 2377 374 146 1280
Fragments arranged by size:
3046 2377 1280 1064 374 162 146 138
EcoICRI GAG'CTC
Cuts at: 3546 3546
Size: 8587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2373 2507 2598 2676 2684 2961
Size: 393 494 247 134 91 78 8 277
Cuts at: 2961 3092 3150 3237 3559 4187 4258 4281 4901
Size: 131 58 87 322 628 71 23 620
Cuts at: 4901 5202 5232 5404 6650 7247 8034 8302 1239
Size: 301 30 172 1246 597 787 268 1524
Fragments arranged by size:
1524 1246 787 628 620 597 494 393
322 301 277 268 247 172 134 131
91 87 78 71 58 30 23 8
EcoO109I rG'GnC_Cy
Cuts at: 2955 4635 4636 5627 5836 2955
Size: 1680 1 991 209 5706
Fragments arranged by size:
5706 1680 991 209 1
EcoRI G'AATT_C
Cuts at: 5896 6274 5896
Size: 378 8209
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2281 2314 2800 3586 5696 2281
Size: 33 486 786 2110 5172
Fragments arranged by size:
5172 2110 786 486 33
FspI TGC'GCA
Cuts at: 7093 7093
Size: 8587
HaeII r_GCGC'y
Cuts at: 1678 4613 4900 7734 1678
Size: 2935 287 2834 2531
Fragments arranged by size:
2935 2834 2531 287
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2153 2185 3530 3562 4142
Size: 32 135 32 581 32 1345 32 580
Cuts at: 4142 4174 4504 4536 4976 5008 5149 5181 5224
Size: 32 330 32 440 32 141 32 43
Cuts at: 5224 5256 7228 7260 7302 7334 1373
Size: 32 1972 32 42 32 2626
Fragments arranged by size:
2626 1972 1345 581 580 440 330 141
135 43 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2890 5931 6177 669
Size: 988 281 952 3041 246 3079
Fragments arranged by size:
3079 3041 988 952 281 246
HindIII A'AGCT_T
Cuts at: 748 3132 3464 4817 748
Size: 2384 332 1353 4518
Fragments arranged by size:
4518 2384 1353 332
HpaI GTT'AAC
Cuts at: 6177 6177
Size: 8587
KasI G'GCGC_C
Cuts at: 4609 4609
Size: 8587
KpnI G_GTAC'C
Cuts at: 5912 5912
Size: 8587
MfeI C'AATT_G
Cuts at: 2560 4735 6186 2560
Size: 2175 1451 4961
Fragments arranged by size:
4961 2175 1451
MluI A'CGCG_T
Cuts at: 4797 8463 4797
Size: 3666 4921
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5491 7701 7885 1305
Size: 4186 2210 184 2007
Fragments arranged by size:
4186 2210 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6765
Fragments arranged by size:
6765 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3068 6545 6904 7063 518
Size: 648 1902 3477 359 159 2042
Fragments arranged by size:
3477 2042 1902 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2393 3045 3369 4113 4145 4616 5507 6003
Size: 787 652 324 744 32 471 891 496
Cuts at: 6003 6680 7828 8073 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 891 787 744 677 652 496
471 324 245 32
NarI GG'CG_CC
Cuts at: 4610 4610
Size: 8587
NcoI C'CATG_G
Cuts at: 513 1065 3649 5752 513
Size: 552 2584 2103 3348
Fragments arranged by size:
3348 2584 2103 552
NdeI CA'TA_TG
Cuts at: 387 5742 5783 387
Size: 5355 41 3191
Fragments arranged by size:
5355 3191 41
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8587
NotI GC'GGCC_GC
Cuts at: 6034 6034
Size: 8587
NruI TCG'CGA
Cuts at: 3874 3874
Size: 8587
NspI r_CATG'y
Cuts at: 1132 1229 2234 5945 7490 1132
Size: 97 1005 3711 1545 2229
Fragments arranged by size:
3711 2229 1545 1005 97
PasI CC'CwG_GG
Cuts at: 1348 5062 1348
Size: 3714 4873
PciI A'CATG_T
Cuts at: 7486 7486
Size: 8587
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2104 2396 5119 5327 1108
Size: 996 292 2723 208 4368
Fragments arranged by size:
4368 2723 996 292 208
PfoI T'CCnGG_A
Cuts at: 2103 5188 2103
Size: 3085 5502
PmeI GTTT'AAAC
Cuts at: 5891 5891
Size: 8587
PmlI CAC'GTG
Cuts at: 5434 5434
Size: 8587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6656 6688 8169 8201 6656
Size: 32 1481 32 7042
Fragments arranged by size:
7042 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2955 5627 2955
Size: 2672 5915
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8587
PsiI TTA'TAA
Cuts at: 6157 6157
Size: 8587
PspOMI G'GGCC_C
Cuts at: 4635 4635
Size: 8587
PspXI vC'TCGA_Gb
Cuts at: 3547 3547
Size: 8587
PstI C_TGCA'G
Cuts at: 830 2161 2200 2919 4605 5939 830
Size: 1331 39 719 1686 1334 3478
Fragments arranged by size:
3478 1686 1334 1331 719 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8587
PvuII CAG'CTG
Cuts at: 2393 3369 4113 4145 4616 5507 2393
Size: 976 744 32 471 891 5473
Fragments arranged by size:
5473 976 891 744 471 32
SacI G_AGCT'C
Cuts at: 3548 3548
Size: 8587
SalI G'TCGA_C
Cuts at: 1936 5929 1936
Size: 3993 4594
SbfI CC_TGCA'GG
Cuts at: 4605 5939 4605
Size: 1334 7253
ScaI AGT'ACT
Cuts at: 5179 5668 6835 8547 5179
Size: 489 1167 1712 5219
Fragments arranged by size:
5219 1712 1167 489
SfcI C'TryA_G
Cuts at: 826 1045 2112 2157 2196 2523 2915 4096 4209
Size: 219 1067 45 39 327 392 1181 113
Cuts at: 4209 4601 5244 5935 7070 7751 7942 826
Size: 392 643 691 1135 681 191 1471
Fragments arranged by size:
1471 1181 1135 1067 691 681 643 392
392 327 219 191 113 45 39
SfoI GGC'GCC
Cuts at: 4611 4611
Size: 8587
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8587
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8587
SmaI CCC'GGG
Cuts at: 1872 5914 1872
Size: 4042 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2451 3547 5425 5670 6691 7592
Size: 197 926 508 1096 1878 245 1021 901
Cuts at: 7592 7854 820
Size: 262 1553
Fragments arranged by size:
1878 1553 1096 1021 926 901 508 262
245 197
SnaBI TAC'GTA
Cuts at: 493 6378 493
Size: 5885 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8587
SphI G_CATG'C
Cuts at: 1132 5945 1132
Size: 4813 3774
SspI AAT'ATT
Cuts at: 5 52 6511 5
Size: 47 6459 2081
Fragments arranged by size:
6459 2081 47
StuI AGG'CCT
Cuts at: 1517 2938 4837 1517
Size: 1421 1899 5267
Fragments arranged by size:
5267 1899 1421
StyI C'CwwG_G
Cuts at: 513 1065 3022 3649 3775 4590 4677 5586 5752
Size: 552 1957 627 126 815 87 909 166
Cuts at: 5752 6026 513
Size: 274 3074
Fragments arranged by size:
3074 1957 909 815 627 552 274 166
126 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3132 4403 4478 4517 5289 6614 6631
Size: 1255 1552 1271 75 39 772 1325 17
Cuts at: 6631 6784 6969 7414 325
Size: 153 185 445 1498
Fragments arranged by size:
1552 1498 1325 1271 1255 772 445 185
153 75 39 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3925 5177 5253
Size: 274 80 33 51 1443 1948 1252 76
Cuts at: 5253 5341 5666 6833 8545 96
Size: 88 325 1167 1712 138
Fragments arranged by size:
1948 1712 1443 1252 1167 325 274 138
88 80 76 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8547 8579 8547
Size: 32 8555
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8587
XbaI T'CTAG_A
Cuts at: 3311 5533 5923 3311
Size: 2222 390 5975
Fragments arranged by size:
5975 2222 390
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5759 5759
Size: 8587
XhoI C'TCGA_G
Cuts at: 1943 3547 1943
Size: 1604 6983
XmaI C'CCGG_G
Cuts at: 1870 5912 1870
Size: 4042 4545
XmnI GAAnn'nnTTC
Cuts at: 3569 5197 6716 3569
Size: 1628 1519 5440
Fragments arranged by size:
5440 1628 1519
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6395 276
Size: 53 83 186 936 4861 2468
Fragments arranged by size:
4861 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI
BstXI BstZ17I EcoNI EcoRV FseI FspAI NaeI NgoMIV
NsiI PacI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI