Restriction map for
pFN21AE3437
(Circular) (Six-base) MAPSORT of: pFN21AE3437.seq Check: 3881 from: 1 to: 7594
pFN21AE3437 7594 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4005 5404 278
Size: 53 83 186 936 2469 1399 2468
Fragments arranged by size:
2469 2468 1399 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3780 4937 1937
Size: 1843 1157 4594
Fragments arranged by size:
4594 1843 1157
Acc65I G'GTAC_C
Cuts at: 4915 4915
Size: 7594
AclI AA'CG_TT
Cuts at: 1563 5721 6094 1563
Size: 4158 373 3063
Fragments arranged by size:
4158 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3625 4227 5657 7041 7309 1632
Size: 1993 602 1430 1384 268 1917
Fragments arranged by size:
1993 1917 1430 1384 602 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7397
AflIII A'CryG_T
Cuts at: 1203 2886 2952 3142 6493 7470 1203
Size: 1683 66 190 3351 977 1327
Fragments arranged by size:
3351 1683 1327 977 190 66
AgeI A'CCGG_T
Cuts at: 6425 6425
Size: 7594
AhdI GACnn_n'nnGTC
Cuts at: 6323 6323
Size: 7594
AleI CACnn'nnGTG
Cuts at: 1166 3401 4373 4535 1166
Size: 2235 972 162 4225
Fragments arranged by size:
4225 2235 972 162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7594
AlwNI CAG_nnn'CTG
Cuts at: 2162 2486 3214 5337 6909 2162
Size: 324 728 2123 1572 2847
Fragments arranged by size:
2847 2123 1572 728 324
ApaI G_GGCC'C
Cuts at: 4014 4062 4014
Size: 48 7546
ApaLI G'TGCA_C
Cuts at: 5651 6807 5651
Size: 1156 6438
ApoI r'AATT_y
Cuts at: 1493 4903 5129 5281 7390 1493
Size: 3410 226 152 2109 1697
Fragments arranged by size:
3410 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6148 160
Size: 872 5116 1606
Fragments arranged by size:
5116 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7594
AvaI C'yCGr_G
Cuts at: 1870 1943 2688 3870 4515 4919 1870
Size: 73 745 1182 645 404 4545
Fragments arranged by size:
4545 1182 745 645 404 73
BamHI G'GATC_C
Cuts at: 3514 4924 5291 3514
Size: 1410 367 5817
Fragments arranged by size:
5817 1410 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2250 2433 4083 4835 4915
Size: 325 217 627 463 183 1650 752 80
Cuts at: 4915 6370 618
Size: 1455 1842
Fragments arranged by size:
1842 1650 1455 752 627 463 325 217
183 80
BanII G_rGCy'C
Cuts at: 1391 2037 2712 3042 3755 4014 4062 4365 4913
Size: 646 675 330 713 259 48 303 548
Cuts at: 4913 1391
Size: 4072
Fragments arranged by size:
4072 713 675 646 548 330 303 259
48
BbeI G_GCGC'C
Cuts at: 2254 2437 4839 2254
Size: 183 2402 5009
Fragments arranged by size:
5009 2402 183
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2078 2724 3490 3631 928
Size: 971 179 646 766 141 4891
Fragments arranged by size:
4891 971 766 646 179 141
BbvCI CC'TCA_GC
Cuts at: 2984 4287 2984
Size: 1303 6291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5785 5819 1903
Size: 34 3848 34 3678
Fragments arranged by size:
3848 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5486 6696 5486
Size: 1210 6384
BclI T'GATC_A
Cuts at: 1548 2817 1548
Size: 1269 6325
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4132 6205 136
Size: 107 122 71 631 605 2460 2073 1525
Fragments arranged by size:
2460 2073 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3567 7589 3567
Size: 4022 3572
BlpI GC'TnA_GC
Cuts at: 5011 5011
Size: 7594
Bme1580I G_kGCm'C
Cuts at: 1790 3410 3870 4014 4062 4088 5655 6811 1790
Size: 1620 460 144 48 26 1567 1156 2573
Fragments arranged by size:
2573 1620 1567 1156 460 144 48 26
BmgBI CAC'GTC
Cuts at: 1318 4798 1318
Size: 3480 4114
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2585 2817 3068
Size: 416 53 83 399 24 1168 232 251
Cuts at: 3068 4029 4252 5325 6283 442
Size: 961 223 1073 958 1753
Fragments arranged by size:
1753 1168 1073 961 958 416 399 251
232 223 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7594
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2359 3053 4089 4406 6254 1239
Size: 1120 694 1036 317 1848 2579
Fragments arranged by size:
2579 1848 1120 1036 694 317
Bpu10I CC'TnA_GC
Cuts at: 2984 4041 4287 2984
Size: 1057 246 6291
Fragments arranged by size:
6291 1057 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2822 3739 5719 6584 6882 2423
Size: 399 917 1980 865 298 3135
Fragments arranged by size:
3135 1980 917 865 399 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2822 4029 4373 6257 882
Size: 617 1323 1207 344 1884 2219
Fragments arranged by size:
2219 1884 1323 1207 617 344
BsaAI yAC'GTr
Cuts at: 493 1204 2353 2955 3931 4297 4321 5385 493
Size: 711 1149 602 976 366 24 1064 2702
Fragments arranged by size:
2702 1149 1064 976 711 602 366 24
BsaBI GATnn'nnATC
Cuts at: 4475 7588 4475
Size: 3113 4481
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2083 2251 2434 4002
Size: 53 83 186 936 550 168 183 1568
Cuts at: 4002 4807 4836 5401 5783 6418 275
Size: 805 29 565 382 635 1451
Fragments arranged by size:
1568 1451 936 805 635 565 550 382
186 183 168 83 53 29
BsaWI w'CCGG_w
Cuts at: 2062 3304 4603 6027 6425 6699 6846 7257 2062
Size: 1242 1299 1424 398 274 147 411 2399
Fragments arranged by size:
2399 1424 1299 1242 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2357 2387 3113 3143 3203
Size: 30 165 30 954 30 726 30 60
Cuts at: 3203 3233 3803 3833 3837 3867 3881 3911 4715
Size: 30 570 30 4 30 14 30 804
Cuts at: 4715 4745 1178
Size: 30 4027
Fragments arranged by size:
4027 954 804 726 570 165 60 30
30 30 30 30 30 30 30 30
14 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 3050 3689 4001 1186
Size: 1051 813 639 312 4779
Fragments arranged by size:
4779 1051 813 639 312
BseYI C'CCAG_C
Cuts at: 2479 2855 3296 4055 4061 4221 4840 4868 6797
Size: 376 441 759 6 160 619 28 1929
Cuts at: 6797 7293 2479
Size: 496 2780
Fragments arranged by size:
2780 1929 759 619 496 441 376 160
28 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2375 1163
Size: 573 639 6382
Fragments arranged by size:
6382 639 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2145 3305 4192 4824 5044 5805
Size: 275 186 144 1160 887 632 220 761
Cuts at: 5805 6833 1540
Size: 1028 2301
Fragments arranged by size:
2301 1160 1028 887 761 632 275 220
186 144
BsiHKAI G_wGCw'C
Cuts at: 3042 4365 5655 5740 6811 3042
Size: 1323 1290 85 1071 3825
Fragments arranged by size:
3825 1323 1290 1071 85
BsmI GAATG_Cn'
Cuts at: 5105 5198 5105
Size: 93 7501
BsmBI CGTCTCn'nnnn_
Cuts at: 4735 4735
Size: 7594
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2712 3042 3410 3755 3870 4014
Size: 399 247 675 330 368 345 115 144
Cuts at: 4014 4062 4088 4365 4913 5655 5740 6811 1391
Size: 48 26 277 548 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 675 548 399 368 345
330 277 247 144 115 85 48 26
BspEI T'CCGG_A
Cuts at: 2062 4603 7257 2062
Size: 2541 2654 2399
Fragments arranged by size:
2654 2541 2399
BspHI T'CATG_A
Cuts at: 2874 2961 5483 7213 2874
Size: 87 2522 1730 3255
Fragments arranged by size:
3255 2522 1730 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3792 4949 844
Size: 2948 1157 3489
Fragments arranged by size:
3489 2948 1157
BsrBI CCG'CTC
Cuts at: 1394 1823 2695 3129 5481 1394
Size: 429 872 434 2352 3507
Fragments arranged by size:
3507 2352 872 434 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2995 3427 4230 6089 6263 65
Size: 1183 1747 432 803 1859 174 1396
Fragments arranged by size:
1859 1747 1396 1183 803 432 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2297 3540 3915 4823 6238 6425 1700
Size: 110 487 1243 375 908 1415 187 2869
Fragments arranged by size:
2869 1415 1243 908 487 375 187 110
BsrGI T'GTAC_A
Cuts at: 96 4611 96
Size: 4515 3079
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7594
BssSI C'ACGA_G
Cuts at: 2219 3545 4535 5654 6666 2219
Size: 1326 990 1119 1012 3147
Fragments arranged by size:
3147 1326 1119 1012 990
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2955 794
Size: 2161 5433
BstBI TT'CG_AA
Cuts at: 6398 6398
Size: 7594
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7594
BstXI CCAn_nnnn'nTGG
Cuts at: 4447 4447
Size: 7594
BstYI r'GATC_y
Cuts at: 1972 3514 3567 3574 4924 5291 5676 5693 7145
Size: 1542 53 7 1350 367 385 17 1452
Cuts at: 7145 7231 7243 7589 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1542 1452 1350 385 367 346 86
53 17 12 7
BtgI C'CryG_G
Cuts at: 513 1065 2004 3379 3510 513
Size: 552 939 1375 131 4597
Fragments arranged by size:
4597 1375 939 552 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2147 3303 4612 487
Size: 683 56 921 1156 1309 3469
Fragments arranged by size:
3469 1309 1156 921 683 56
BtsI GCAGTG_nn'
Cuts at: 2288 2345 2984 3464 5110 5904 5924 2288
Size: 57 639 480 1646 794 20 3958
Fragments arranged by size:
3958 1646 794 639 480 57 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7594
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7559
DraI TTT'AAA
Cuts at: 4898 4898
Size: 7594
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4727 6601 809
Size: 507 3411 1874 1802
Fragments arranged by size:
3411 1874 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2527 3118 3163
Size: 54 1425 50 275 18 697 591 45
Cuts at: 3163 4699 4821 5041 5930 7559 8
Size: 1536 122 220 889 1629 43
Fragments arranged by size:
1629 1536 1425 889 697 591 275 220
122 54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4821 5041 1537
Size: 275 3009 220 4090
Fragments arranged by size:
4090 3009 275 220
EarI CTCTTCn'nnn_
Cuts at: 1353 2113 2889 3392 4370 4784 5524 1353
Size: 760 776 503 978 414 740 3423
Fragments arranged by size:
3423 978 776 760 740 503 414
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3364 6181 6555 6701 387
Size: 1064 138 1775 2817 374 146 1280
Fragments arranged by size:
2817 1775 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3040 4363 3040
Size: 1323 6271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2359 3053 3625 4089 4227 4406 5657
Size: 393 727 694 572 464 138 179 1251
Cuts at: 5657 6254 7041 7309 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1251 787 727 694 597 572 464
393 268 179 138
EcoNI CCTnn'n_nnAGG
Cuts at: 2441 4388 2441
Size: 1947 5647
EcoO109I rG'GnC_Cy
Cuts at: 3247 3892 4011 4555 3247
Size: 645 119 544 6286
Fragments arranged by size:
6286 645 544 119
EcoRI G'AATT_C
Cuts at: 4903 5281 4903
Size: 378 7216
EcoRV GAT'ATC
Cuts at: 3709 3709
Size: 7594
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3505 3505
Size: 7594
FspI TGC'GCA
Cuts at: 2681 6100 2681
Size: 3419 4175
HaeII r_GCGC'y
Cuts at: 1678 2254 2437 4839 6741 1678
Size: 576 183 2402 1902 2531
Fragments arranged by size:
2531 2402 1902 576 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2357 2389 3203 3235 3559
Size: 32 135 32 785 32 814 32 324
Cuts at: 3559 3591 3750 3782 3837 3869 6235 6267 6309
Size: 32 159 32 55 32 2366 32 42
Cuts at: 6309 6341 1373
Size: 32 2626
Fragments arranged by size:
2626 2366 814 785 324 159 135 55
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4938 5184 669
Size: 988 281 3000 246 3079
Fragments arranged by size:
3079 3000 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7594
HpaI GTT'AAC
Cuts at: 5184 5184
Size: 7594
KasI G'GCGC_C
Cuts at: 2250 2433 4835 2250
Size: 183 2402 5009
Fragments arranged by size:
5009 2402 183
KpnI G_GTAC'C
Cuts at: 4919 4919
Size: 7594
MfeI C'AATT_G
Cuts at: 4102 5193 4102
Size: 1091 6503
MluI A'CGCG_T
Cuts at: 7470 7470
Size: 7594
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2871 4662 6708 6892 1305
Size: 1566 1791 2046 184 2007
Fragments arranged by size:
2046 2007 1791 1566 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2529 3120 3165 10
Size: 54 1425 343 697 591 45 4439
Fragments arranged by size:
4439 1425 697 591 343 54 45
MslI CAynn'nnrTG
Cuts at: 518 1166 3401 4120 4373 4535 5552 5911 6070
Size: 648 2235 719 253 162 1017 359 159
Cuts at: 6070 518
Size: 2042
Fragments arranged by size:
2235 2042 1017 719 648 359 253 162
159
MspA1I CmG'CkG
Cuts at: 1606 2103 2159 2189 2486 2508 2866 4150 4432
Size: 497 56 30 297 22 358 1284 282
Cuts at: 4432 4698 5010 5687 6835 7080 1606
Size: 266 312 677 1148 245 2120
Fragments arranged by size:
2120 1284 1148 677 497 358 312 297
282 266 245 56 30 22
NaeI GCC'GGC
Cuts at: 2299 4825 2299
Size: 2526 5068
NarI GG'CG_CC
Cuts at: 2251 2434 4836 2251
Size: 183 2402 5009
Fragments arranged by size:
5009 2402 183
NcoI C'CATG_G
Cuts at: 513 1065 2004 3510 513
Size: 552 939 1506 4597
Fragments arranged by size:
4597 1506 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7594
NgoMIV G'CCGG_C
Cuts at: 2297 4823 2297
Size: 2526 5068
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7594
NotI GC'GGCC_GC
Cuts at: 5041 5041
Size: 7594
NspI r_CATG'y
Cuts at: 1132 1229 2873 2890 3146 4657 4952 6497 1132
Size: 97 1644 17 256 1511 295 1545 2229
Fragments arranged by size:
2229 1644 1545 1511 295 256 97 17
PasI CC'CwG_GG
Cuts at: 1348 4007 1348
Size: 2659 4935
PciI A'CATG_T
Cuts at: 2886 3142 6493 2886
Size: 256 3351 3987
Fragments arranged by size:
3987 3351 256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3184 4798 1108
Size: 2076 1614 3904
Fragments arranged by size:
3904 2076 1614
PfoI T'CCnGG_A
Cuts at: 2049 3228 4095 4551 2049
Size: 1179 867 456 5092
Fragments arranged by size:
5092 1179 867 456
PmeI GTTT'AAAC
Cuts at: 4898 4898
Size: 7594
PmlI CAC'GTG
Cuts at: 2955 2955
Size: 7594
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5663 5695 7176 7208 5663
Size: 32 1481 32 6049
Fragments arranged by size:
6049 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3247 4555 3247
Size: 1308 6286
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7594
PsiI TTA'TAA
Cuts at: 5164 5164
Size: 7594
PspOMI G'GGCC_C
Cuts at: 4010 4058 4010
Size: 48 7546
PstI C_TGCA'G
Cuts at: 830 2979 4164 4350 4859 4946 830
Size: 2149 1185 186 509 87 3478
Fragments arranged by size:
3478 2149 1185 509 186 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7594
PvuII CAG'CTG
Cuts at: 2103 2159 2486 2508 2866 4150 4432 2103
Size: 56 327 22 358 1284 282 5265
Fragments arranged by size:
5265 1284 358 327 282 56 22
SacI G_AGCT'C
Cuts at: 3042 4365 3042
Size: 1323 6271
SalI G'TCGA_C
Cuts at: 1936 4936 1936
Size: 3000 4594
SapI GCTCTTCn'nnn_
Cuts at: 4370 4370
Size: 7594
SbfI CC_TGCA'GG
Cuts at: 4946 4946
Size: 7594
ScaI AGT'ACT
Cuts at: 3011 5842 7554 3011
Size: 2831 1712 3051
Fragments arranged by size:
3051 2831 1712
SexAI A'CCwGG_T
Cuts at: 2499 3096 2499
Size: 597 6997
SfcI C'TryA_G
Cuts at: 826 1045 2113 2975 4160 4346 4855 4942 6077
Size: 219 1068 862 1185 186 509 87 1135
Cuts at: 6077 6758 6949 826
Size: 681 191 1471
Fragments arranged by size:
1471 1185 1135 1068 862 681 509 219
191 186 87
SfoI GGC'GCC
Cuts at: 2252 2435 4837 2252
Size: 183 2402 5009
Fragments arranged by size:
5009 2402 183
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7594
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7594
SmaI CCC'GGG
Cuts at: 1872 3872 4921 1872
Size: 2000 1049 4545
Fragments arranged by size:
4545 2000 1049
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2438 2801 3754 5698 6599 6861
Size: 197 926 495 363 953 1944 901 262
Cuts at: 6861 820
Size: 1553
Fragments arranged by size:
1944 1553 953 926 901 495 363 262
197
SnaBI TAC'GTA
Cuts at: 493 5385 493
Size: 4892 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7594
SphI G_CATG'C
Cuts at: 1132 4657 4952 1132
Size: 3525 295 3774
Fragments arranged by size:
3774 3525 295
SspI AAT'ATT
Cuts at: 5 52 5518 5
Size: 47 5466 2081
Fragments arranged by size:
5466 2081 47
StuI AGG'CCT
Cuts at: 1517 2235 3086 1517
Size: 718 851 6025
Fragments arranged by size:
6025 851 718
StyI C'CwwG_G
Cuts at: 513 1065 2004 3510 4108 4130 4761 5033 513
Size: 552 939 1506 598 22 631 272 3074
Fragments arranged by size:
3074 1506 939 631 598 552 272 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2551 4882 5621 5638 5791 5976 6421
Size: 1255 971 2331 739 17 153 185 445
Cuts at: 6421 325
Size: 1498
Fragments arranged by size:
2331 1498 1255 971 739 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3009 4611 5840
Size: 274 80 33 51 1443 1032 1602 1229
Cuts at: 5840 7552 96
Size: 1712 138
Fragments arranged by size:
1712 1602 1443 1229 1032 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2231 2263 3113 3145 7554 7586 2231
Size: 32 850 32 4409 32 2239
Fragments arranged by size:
4409 2239 850 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4310 1316
Size: 2994 4600
XbaI T'CTAG_A
Cuts at: 2518 4930 2518
Size: 2412 5182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2487 2487
Size: 7594
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7594
XmaI C'CCGG_G
Cuts at: 1870 3870 4919 1870
Size: 2000 1049 4545
Fragments arranged by size:
4545 2000 1049
XmnI GAAnn'nnTTC
Cuts at: 5723 5723
Size: 7594
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4003 5402 276
Size: 53 83 186 936 2469 1399 2468
Fragments arranged by size:
2469 2468 1399 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII FseI FspAI NruI NsiI
PacI PspXI PsrI RsrII SacII SanDI SfiI SrfI
SwaI