Restriction map for
pFN21AE3436
(Circular) (Six-base) MAPSORT of: pFN21AE3436.seq Check: 6200 from: 1 to: 7492
pFN21AE3436 7492 bp
With 182 enzymes: SgfI *
March 28, 2012 14:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5302 278
Size: 53 83 186 936 3766 2468
Fragments arranged by size:
3766 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2110 3493 4835 1937
Size: 173 1383 1342 4594
Fragments arranged by size:
4594 1383 1342 173
Acc65I G'GTAC_C
Cuts at: 3160 4813 3160
Size: 1653 5839
AclI AA'CG_TT
Cuts at: 1563 5619 5992 1563
Size: 4056 373 3063
Fragments arranged by size:
4056 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2465 2511 3029 3120 4332 5555 6939 7207
Size: 833 46 518 91 1212 1223 1384 268
Cuts at: 7207 1632
Size: 1917
Fragments arranged by size:
1917 1384 1223 1212 833 518 268 91
46
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7295
AflIII A'CryG_T
Cuts at: 1203 2911 6391 7368 1203
Size: 1708 3480 977 1327
Fragments arranged by size:
3480 1708 1327 977
AgeI A'CCGG_T
Cuts at: 6323 6323
Size: 7492
AhdI GACnn_n'nnGTC
Cuts at: 6221 6221
Size: 7492
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7492
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7492
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3612 3644 3612
Size: 32 7460
AlwNI CAG_nnn'CTG
Cuts at: 4090 5235 6807 4090
Size: 1145 1572 4775
Fragments arranged by size:
4775 1572 1145
ApaLI G'TGCA_C
Cuts at: 4121 5549 6705 4121
Size: 1428 1156 4908
Fragments arranged by size:
4908 1428 1156
ApoI r'AATT_y
Cuts at: 1493 2096 2231 2639 2665 3117 3140 3523 3606
Size: 603 135 408 26 452 23 383 83
Cuts at: 3606 3639 4306 4801 5027 5179 7288 1493
Size: 33 667 495 226 152 2109 1697
Fragments arranged by size:
2109 1697 667 603 495 452 408 383
226 152 135 83 33 26 23
AseI AT'TA_AT
Cuts at: 160 1032 3227 6046 160
Size: 872 2195 2819 1606
Fragments arranged by size:
2819 2195 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7492
AvaI C'yCGr_G
Cuts at: 1870 1943 4817 1870
Size: 73 2874 4545
Fragments arranged by size:
4545 2874 73
BamHI G'GATC_C
Cuts at: 4822 5189 4822
Size: 367 7125
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3160 4813 6268 618
Size: 325 217 627 1373 1653 1455 1842
Fragments arranged by size:
1842 1653 1455 1373 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3405 4811 1391
Size: 2014 1406 4072
Fragments arranged by size:
4072 2014 1406
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2675 928
Size: 971 776 5745
Fragments arranged by size:
5745 971 776
BbvCI CC'TCA_GC
Cuts at: 3195 3195
Size: 7492
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5683 5717 1903
Size: 34 3746 34 3678
Fragments arranged by size:
3746 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5384 6594 5384
Size: 1210 6282
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7492
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6103 136
Size: 107 122 71 631 605 4431 1525
Fragments arranged by size:
4431 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4780 7487 4780
Size: 2707 4785
BlpI GC'TnA_GC
Cuts at: 4909 4909
Size: 7492
Bme1580I G_kGCm'C
Cuts at: 1790 4125 5553 6709 1790
Size: 2335 1428 1156 2573
Fragments arranged by size:
2573 2335 1428 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7492
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2410 5223 6181
Size: 416 53 83 399 24 993 2813 958
Cuts at: 6181 442
Size: 1753
Fragments arranged by size:
2813 1753 993 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2141 4767 1056
Size: 1085 2626 3781
Fragments arranged by size:
3781 2626 1085
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4488 4488
Size: 7492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2412 3854 6152 1239
Size: 1173 1442 2298 2579
Fragments arranged by size:
2579 2298 1442 1173
Bpu10I CC'TnA_GC
Cuts at: 3195 3538 3195
Size: 343 7149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4602 5617 6482 6780 4602
Size: 1015 865 298 5314
Fragments arranged by size:
5314 1015 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6155 882
Size: 617 4656 2219
Fragments arranged by size:
4656 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5283 493
Size: 711 4079 2702
Fragments arranged by size:
4079 2702 711
BsaBI GATnn'nnATC
Cuts at: 2958 7486 2958
Size: 4528 2964
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5299 5681 6316 275
Size: 53 83 186 936 3766 382 635 1451
Fragments arranged by size:
3766 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5925 6323 6597 6744 7155 5925
Size: 398 274 147 411 6262
Fragments arranged by size:
6262 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7267
Fragments arranged by size:
7267 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2283 3917 4605 1186
Size: 1097 1634 688 4073
Fragments arranged by size:
4073 1634 1097 688
BseYI C'CCAG_C
Cuts at: 6695 7191 6695
Size: 496 6996
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3018 3131 1163
Size: 573 1282 113 5524
Fragments arranged by size:
5524 1282 573 113
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4942 5703 6731 1540
Size: 275 186 2941 761 1028 2301
Fragments arranged by size:
2941 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3373 3405 4125 5553 5638 6709 3373
Size: 32 720 1428 85 1071 4156
Fragments arranged by size:
4156 1428 1071 720 85 32
BsmI GAATG_Cn'
Cuts at: 3206 5003 5096 3206
Size: 1797 93 5602
Fragments arranged by size:
5602 1797 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3373 3405 4125 4811 5553 5638 6709
Size: 399 1583 32 720 686 742 85 1071
Cuts at: 6709 1391
Size: 2174
Fragments arranged by size:
2174 1583 1071 742 720 686 399 85
32
BspEI T'CCGG_A
Cuts at: 7155 7155
Size: 7492
BspHI T'CATG_A
Cuts at: 5381 7111 5381
Size: 1730 5762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3631 4847 844
Size: 2787 1216 3489
Fragments arranged by size:
3489 2787 1216
BsrBI CCG'CTC
Cuts at: 1394 1823 5379 1394
Size: 429 3556 3507
Fragments arranged by size:
3556 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2115 2137 4774 5987 6161 65
Size: 1183 867 22 2637 1213 174 1396
Fragments arranged by size:
2637 1396 1213 1183 867 174 22
BsrFI r'CCGG_y
Cuts at: 1700 1810 6136 6323 1700
Size: 110 4326 187 2869
Fragments arranged by size:
4326 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2357 96
Size: 2261 5231
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7492
BssSI C'ACGA_G
Cuts at: 5552 6564 5552
Size: 1012 6480
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7492
BstBI TT'CG_AA
Cuts at: 6296 6296
Size: 7492
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7492
BstXI CCAn_nnnn'nTGG
Cuts at: 4593 4593
Size: 7492
BstYI r'GATC_y
Cuts at: 1972 2870 3393 4780 4822 5189 5574 5591 7043
Size: 898 523 1387 42 367 385 17 1452
Cuts at: 7043 7129 7141 7487 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1387 898 523 385 367 346
86 42 17 12
Bsu36I CC'TnA_GG
Cuts at: 2895 2895
Size: 7492
BtgI C'CryG_G
Cuts at: 513 1065 2004 3357 4257 513
Size: 552 939 1353 900 3748
Fragments arranged by size:
3748 1353 939 900 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6753
Fragments arranged by size:
6753 683 56
BtsI GCAGTG_nn'
Cuts at: 2521 3005 3988 5008 5802 5822 2521
Size: 484 983 1020 794 20 4191
Fragments arranged by size:
4191 1020 983 794 484 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7492
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7457
DraI TTT'AAA
Cuts at: 4776 4796 4776
Size: 20 7472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6499 809
Size: 507 5183 1802
Fragments arranged by size:
5183 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3702 4939 5828
Size: 54 1425 50 275 18 1872 1237 889
Cuts at: 5828 7457 8
Size: 1629 43
Fragments arranged by size:
1872 1629 1425 1237 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4939 1537
Size: 275 3127 4090
Fragments arranged by size:
4090 3127 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 5422 1353
Size: 671 3398 3423
Fragments arranged by size:
3423 3398 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6079 6453 6599 387
Size: 1064 138 4490 374 146 1280
Fragments arranged by size:
4490 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3403 3403
Size: 7492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2412 2465 2511 3029 3120 3854 4332
Size: 393 780 53 46 518 91 734 478
Cuts at: 4332 5555 6152 6939 7207 1239
Size: 1223 597 787 268 1524
Fragments arranged by size:
1524 1223 787 780 734 597 518 478
393 268 91 53 46
EcoNI CCTnn'n_nnAGG
Cuts at: 4617 4617
Size: 7492
EcoO109I rG'GnC_Cy
Cuts at: 2659 2659
Size: 7492
EcoRI G'AATT_C
Cuts at: 4801 5179 4801
Size: 378 7114
EcoRV GAT'ATC
Cuts at: 3586 3586
Size: 7492
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2995 3815 2995
Size: 820 6672
FspI TGC'GCA
Cuts at: 5998 5998
Size: 7492
HaeII r_GCGC'y
Cuts at: 1678 6639 1678
Size: 4961 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3434 3466 4169 4201 6133
Size: 32 135 32 1862 32 703 32 1932
Cuts at: 6133 6165 6207 6239 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1932 1862 703 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2111 2172 2283 2812 4836 5082
Size: 988 281 173 61 111 529 2024 246
Cuts at: 5082 669
Size: 3079
Fragments arranged by size:
3079 2024 988 529 281 246 173 111
61
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7492
HpaI GTT'AAC
Cuts at: 2283 5082 2283
Size: 2799 4693
KpnI G_GTAC'C
Cuts at: 3164 4817 3164
Size: 1653 5839
MfeI C'AATT_G
Cuts at: 5091 5091
Size: 7492
MluI A'CGCG_T
Cuts at: 7368 7368
Size: 7492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2299 2829 2963 3446 4387 4652 6606 6790
Size: 994 530 134 483 941 265 1954 184
Cuts at: 6790 1305
Size: 2007
Fragments arranged by size:
2007 1954 994 941 530 483 265 184
134
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5670
Fragments arranged by size:
5670 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2514 3532 4172 4268 4511 5450 5809
Size: 648 1348 1018 640 96 243 939 359
Cuts at: 5809 5968 518
Size: 159 2042
Fragments arranged by size:
2042 1348 1018 939 648 640 359 243
159 96
MspA1I CmG'CkG
Cuts at: 1606 2054 3700 4908 5585 6733 6978 1606
Size: 448 1646 1208 677 1148 245 2120
Fragments arranged by size:
2120 1646 1208 1148 677 448 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3357 4257 513
Size: 552 939 1353 900 3748
Fragments arranged by size:
3748 1353 939 900 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7492
NheI G'CTAG_C
Cuts at: 1052 2137 4763 1052
Size: 1085 2626 3781
Fragments arranged by size:
3781 2626 1085
NotI GC'GGCC_GC
Cuts at: 4939 4939
Size: 7492
NspI r_CATG'y
Cuts at: 1132 1229 2915 4363 4850 6395 1132
Size: 97 1686 1448 487 1545 2229
Fragments arranged by size:
2229 1686 1545 1448 487 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7492
PciI A'CATG_T
Cuts at: 2911 6391 2911
Size: 3480 4012
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7492
PfoI T'CCnGG_A
Cuts at: 3396 3396
Size: 7492
PmeI GTTT'AAAC
Cuts at: 4796 4796
Size: 7492
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5561 5593 7074 7106 5561
Size: 32 1481 32 5947
Fragments arranged by size:
5947 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2659 2659
Size: 7492
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7492
PsiI TTA'TAA
Cuts at: 2445 5062 2445
Size: 2617 4875
PstI C_TGCA'G
Cuts at: 830 4844 830
Size: 4014 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7492
PvuII CAG'CTG
Cuts at: 2054 3700 2054
Size: 1646 5846
SacI G_AGCT'C
Cuts at: 3405 3405
Size: 7492
SalI G'TCGA_C
Cuts at: 1936 2109 4834 1936
Size: 173 2725 4594
Fragments arranged by size:
4594 2725 173
SanDI GG'GwC_CC
Cuts at: 2659 2659
Size: 7492
SbfI CC_TGCA'GG
Cuts at: 4844 4844
Size: 7492
ScaI AGT'ACT
Cuts at: 2035 4237 5740 7452 2035
Size: 2202 1503 1712 2075
Fragments arranged by size:
2202 2075 1712 1503
SfcI C'TryA_G
Cuts at: 826 1045 4387 4840 5975 6656 6847 826
Size: 219 3342 453 1135 681 191 1471
Fragments arranged by size:
3342 1471 1135 681 453 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7492
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7492
SmaI CCC'GGG
Cuts at: 1872 4819 1872
Size: 2947 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4617 5596 6497 6759 820
Size: 197 926 2674 979 901 262 1553
Fragments arranged by size:
2674 1553 979 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5283 493
Size: 4790 2702
SpeI A'CTAG_T
Cuts at: 152 2063 152
Size: 1911 5581
SphI G_CATG'C
Cuts at: 1132 4850 1132
Size: 3718 3774
SspI AAT'ATT
Cuts at: 5 52 2699 3014 3922 5416 5
Size: 47 2647 315 908 1494 2081
Fragments arranged by size:
2647 2081 1494 908 315 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7492
StyI C'CwwG_G
Cuts at: 513 1065 2004 3357 3615 4257 4931 513
Size: 552 939 1353 258 642 674 3074
Fragments arranged by size:
3074 1353 939 674 642 552 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5519 5536 5689 5874 6319 325
Size: 1255 3939 17 153 185 445 1498
Fragments arranged by size:
3939 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2033 2357 3574
Size: 274 80 33 51 1443 56 324 1217
Cuts at: 3574 3659 4235 5738 7450 96
Size: 85 576 1503 1712 138
Fragments arranged by size:
1712 1503 1443 1217 576 324 274 138
85 80 56 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4234 4266 7452 7484 4234
Size: 32 3186 32 4242
Fragments arranged by size:
4242 3186 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7492
XbaI T'CTAG_A
Cuts at: 4828 4828
Size: 7492
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2344 3697 4423 2344
Size: 1353 726 5413
Fragments arranged by size:
5413 1353 726
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7492
XmaI C'CCGG_G
Cuts at: 1870 4817 1870
Size: 2947 4545
XmnI GAAnn'nnTTC
Cuts at: 5621 5621
Size: 7492
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5300 276
Size: 53 83 186 936 3766 2468
Fragments arranged by size:
3766 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI